AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072765

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072765.t1 MTR_5g010000 87.866 478 22 3 2 443 27 504 0.0 801
MS.gene072765.t1 MTR_5g009970 77.521 476 71 3 1 440 27 502 0.0 723
MS.gene072765.t1 MTR_5g009970 80.523 421 62 2 40 440 8 428 0.0 674
MS.gene072765.t1 MTR_5g009970 80.050 401 60 2 60 440 1 401 0.0 634
MS.gene072765.t1 MTR_5g009970 78.979 333 50 2 128 440 1 333 1.53e-180 507
MS.gene072765.t1 MTR_3g080810 48.504 468 193 8 15 437 35 499 9.93e-150 435
MS.gene072765.t1 MTR_1g088190 47.768 448 201 7 12 431 36 478 7.76e-141 412
MS.gene072765.t1 MTR_3g117500 46.170 470 205 7 6 429 15 482 9.74e-141 412
MS.gene072765.t1 MTR_8g104820 45.182 467 204 10 11 429 26 488 2.83e-138 405
MS.gene072765.t1 MTR_5g085730 43.633 479 215 9 6 432 22 497 4.02e-135 399
MS.gene072765.t1 MTR_3g080190 43.478 483 218 9 11 441 36 515 7.51e-133 393
MS.gene072765.t1 MTR_5g093590 43.830 470 210 9 14 431 33 500 2.07e-130 386
MS.gene072765.t1 MTR_4g035955 43.871 465 217 6 5 429 4 464 6.44e-130 384
MS.gene072765.t1 MTR_3g010330 45.085 468 210 9 9 433 19 482 1.79e-129 383
MS.gene072765.t1 MTR_3g105230 42.601 446 217 6 12 421 27 469 4.27e-129 382
MS.gene072765.t1 MTR_4g035930 43.297 455 214 6 15 429 15 465 2.31e-128 379
MS.gene072765.t1 MTR_8g099410 43.460 474 213 8 11 433 24 493 4.25e-127 378
MS.gene072765.t1 MTR_3g023740 43.612 454 217 6 14 431 29 479 4.83e-127 377
MS.gene072765.t1 MTR_4g074960 43.497 469 211 8 14 431 27 492 1.16e-126 380
MS.gene072765.t1 MTR_8g099410 43.460 474 213 8 11 433 24 493 6.38e-126 379
MS.gene072765.t1 MTR_4g035975 42.704 466 213 8 14 429 26 487 4.12e-125 372
MS.gene072765.t1 MTR_1g037340 42.826 460 217 7 15 431 25 481 1.18e-119 358
MS.gene072765.t1 MTR_3g113720 38.947 475 235 7 16 441 58 526 3.80e-109 332
MS.gene072765.t1 MTR_8g075990 39.400 467 230 6 16 433 64 526 4.03e-105 322
MS.gene072765.t1 MTR_2g028480 36.456 491 232 11 15 434 111 592 1.13e-83 269
MS.gene072765.t1 MTR_3g090540 34.012 491 246 15 15 436 107 588 1.08e-80 261
MS.gene072765.t1 MTR_3g082440 34.623 491 241 14 15 437 116 594 2.90e-76 249
MS.gene072765.t1 MTR_1g088190 45.890 292 126 6 12 275 36 323 1.38e-73 234
MS.gene072765.t1 MTR_7g108925 45.882 170 88 2 275 441 43 211 7.36e-51 172
MS.gene072765.t1 MTR_0393s0050 48.276 145 73 2 275 418 20 163 3.47e-39 140
MS.gene072765.t1 MTR_1g037320 51.639 122 57 2 309 429 169 289 3.51e-33 127
MS.gene072765.t1 MTR_1g037320 47.059 136 72 0 60 195 1 136 1.75e-32 125
MS.gene072765.t1 MTR_4g128810 42.675 157 84 2 284 440 189 339 1.81e-32 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072765.t1 AT4G23560 64.795 463 128 2 5 432 13 475 0.0 593
MS.gene072765.t1 AT4G09740 63.931 463 132 2 5 432 13 475 0.0 585
MS.gene072765.t1 AT1G02800 49.676 463 184 8 13 431 41 498 1.44e-150 437
MS.gene072765.t1 AT4G02290 46.753 462 199 7 12 429 49 507 5.67e-144 421
MS.gene072765.t1 AT1G23210 46.681 467 200 8 5 426 18 480 1.16e-143 419
MS.gene072765.t1 AT1G70710 46.739 460 198 7 11 426 24 480 1.97e-142 416
MS.gene072765.t1 AT4G39010 46.269 469 203 8 8 429 22 488 5.02e-141 413
MS.gene072765.t1 AT1G22880 45.572 463 206 8 9 429 18 476 7.16e-132 389
MS.gene072765.t1 AT1G71380 45.708 466 209 8 5 429 14 476 1.92e-131 388
MS.gene072765.t1 AT2G32990 42.275 466 218 8 14 429 38 502 1.67e-128 382
MS.gene072765.t1 AT3G43860 43.929 453 215 6 15 431 34 483 2.84e-127 377
MS.gene072765.t1 AT3G43860 43.929 453 215 6 15 431 34 483 2.84e-127 377
MS.gene072765.t1 AT4G11050 41.580 481 225 9 12 440 23 499 5.00e-124 371
MS.gene072765.t1 AT4G39000 43.011 465 212 9 14 429 24 484 1.34e-123 368
MS.gene072765.t1 AT4G11050 41.580 481 225 9 12 440 23 499 2.37e-123 372
MS.gene072765.t1 AT4G11050 41.580 481 225 9 12 440 23 499 2.54e-123 372
MS.gene072765.t1 AT1G48930 45.259 464 205 7 14 429 29 491 4.26e-121 366
MS.gene072765.t1 AT1G64390 41.401 471 221 8 12 431 22 488 5.62e-121 366
MS.gene072765.t1 AT4G38990 42.038 471 221 8 10 429 18 487 1.50e-120 360
MS.gene072765.t1 AT2G44570 43.226 465 218 8 11 431 29 491 1.82e-120 360
MS.gene072765.t1 AT2G44540 43.167 461 217 7 15 432 33 491 2.96e-118 354
MS.gene072765.t1 AT4G02290 44.975 398 188 6 60 429 1 395 1.18e-117 350
MS.gene072765.t1 AT2G44560 43.261 460 216 7 15 431 33 490 1.04e-116 350
MS.gene072765.t1 AT2G44550 41.597 476 224 10 1 432 25 490 2.49e-114 344
MS.gene072765.t1 AT1G19940 41.575 457 218 6 15 426 48 500 7.08e-114 344
MS.gene072765.t1 AT1G19940 41.575 457 218 6 15 426 48 500 7.08e-114 344
MS.gene072765.t1 AT1G75680 41.501 453 214 6 16 422 58 505 1.65e-109 333
MS.gene072765.t1 AT1G22880 44.949 396 188 7 60 429 1 392 2.14e-108 326
MS.gene072765.t1 AT4G39010 41.848 368 165 8 8 328 22 387 1.15e-91 283
MS.gene072765.t1 AT5G49720 36.122 490 235 11 15 434 108 589 7.10e-82 265
MS.gene072765.t1 AT4G24260 35.234 491 238 11 15 434 108 589 6.30e-77 251
MS.gene072765.t1 AT2G44570 38.462 377 187 7 11 344 29 403 1.97e-76 244
MS.gene072765.t1 AT1G19940 38.611 360 176 5 15 329 48 407 1.71e-74 239
MS.gene072765.t1 AT1G65610 32.165 485 251 13 15 431 116 590 3.96e-74 244
MS.gene072765.t1 AT1G22880 43.042 309 131 7 9 275 18 323 5.75e-70 225
MS.gene072765.t1 AT4G38990 38.554 332 179 7 5 313 13 342 4.58e-63 207
MS.gene072765.t1 AT4G24260 31.606 386 188 10 15 329 108 488 1.14e-39 149
MS.gene072765.t1 AT4G24260 35.439 285 118 7 15 236 108 389 8.81e-36 137
MS.gene072765.t1 AT3G42153 47.727 88 42 1 190 273 20 107 3.30e-21 89.0

Find 94 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCATACATGGTGGGATA+TGG 0.230083 5.3:+3730808 MS.gene072765:CDS
CCAGAGTACGAGCAGTAAAA+TGG 0.238707 5.3:-3729884 None:intergenic
TCAGCTGCAGCCTCAGTTCC+AGG 0.287596 5.3:-3729494 None:intergenic
TTGTATTGGTCATTAGAATT+TGG 0.289530 5.3:-3730982 None:intergenic
CCCACATGAATATTAGGATT+TGG 0.290939 5.3:-3730943 None:intergenic
TCAATCTTGTAAGTTGTTCT+TGG 0.295072 5.3:-3729461 None:intergenic
CCTCTGTGATGTAATTGTTT+TGG 0.305598 5.3:-3730841 None:intergenic
CTTGGTGTATCCATGTCTTC+TGG 0.334811 5.3:-3729443 None:intergenic
ACATAGGTGGACTACATATT+AGG 0.343969 5.3:+3730769 MS.gene072765:intron
ATCTGTAGAGAGCTACTTCC+TGG 0.344038 5.3:-3730371 None:intergenic
ATAGCACCCACATGAATATT+AGG 0.348295 5.3:-3730949 None:intergenic
GTGTCATTGGCAGGTGGATT+TGG 0.356090 5.3:+3729055 MS.gene072765:intron
CATTGGCAGGTGGATTTGGT+TGG 0.371328 5.3:+3729059 MS.gene072765:intron
TCTTCGCAGTGCTATTCGTT+GGG 0.375871 5.3:+3729205 MS.gene072765:CDS
AGCTGGGATAACAAATTTGT+TGG 0.384877 5.3:+3730125 MS.gene072765:CDS
CAGATCTTGCATCATTGTAT+TGG 0.398459 5.3:-3730996 None:intergenic
GGTTCTGTTGCAGCTTTGCT+TGG 0.409254 5.3:+3731066 MS.gene072765:CDS
CACCATGTATGACATCTTCA+TGG 0.417078 5.3:-3730800 None:intergenic
TCATTGCACCCCACTTTGGC+AGG 0.417272 5.3:-3730892 None:intergenic
TTCACCATCAGTCTTAATCT+TGG 0.417439 5.3:-3730327 None:intergenic
ATCTTCGCAGTGCTATTCGT+TGG 0.429802 5.3:+3729204 MS.gene072765:CDS
CACTTCACTCTTTGGTCCTT+AGG 0.430996 5.3:-3728859 None:intergenic
TGGCAGGTGGATTTGGTTGG+AGG 0.444755 5.3:+3729062 MS.gene072765:intron
TGGAACTGAGGCTGCAGCTG+AGG 0.459683 5.3:+3729496 MS.gene072765:CDS
ATTCATGTGGGTGCTATTGT+TGG 0.466637 5.3:+3730955 MS.gene072765:CDS
AAGCAGTGTCTGAATTCAGC+TGG 0.468104 5.3:+3730108 MS.gene072765:CDS
GAAACGCAGATCATAATTGT+TGG 0.470658 5.3:+3729414 MS.gene072765:CDS
TAGATGAAGAGAAATAGTTC+TGG 0.473162 5.3:-3730918 None:intergenic
GCGGGTGATAATGTGAAGTT+TGG 0.476392 5.3:+3729095 MS.gene072765:CDS
GCCATCATTGCACCCCACTT+TGG 0.479110 5.3:-3730896 None:intergenic
TTGAGATATGATTGAAGGGA+TGG 0.481296 5.3:-3730869 None:intergenic
TTACGGTGAGCTTGTTGAGT+TGG 0.481846 5.3:+3729132 MS.gene072765:CDS
GCCAAAGTGGGGTGCAATGA+TGG 0.482616 5.3:+3730895 MS.gene072765:CDS
GCAGGTTGAGATATGATTGA+AGG 0.483094 5.3:-3730874 None:intergenic
TGCAAAGGCTCTAATTTCAG+AGG 0.483766 5.3:-3730662 None:intergenic
TGTCATACATGGTGGGATAT+GGG 0.486645 5.3:+3730809 MS.gene072765:CDS
CAGAGTACGAGCAGTAAAAT+GGG 0.494790 5.3:-3729883 None:intergenic
ATATGAAAGTGAGATTTCTT+CGG 0.496777 5.3:+3729166 MS.gene072765:CDS
AACAAAAGCAGCATTCATAT+AGG 0.500246 5.3:-3731043 None:intergenic
AAGATTGATTCGAATTCCCC+TGG 0.503739 5.3:+3729476 MS.gene072765:CDS
ACACTGCTTGACTCCAACCT+TGG 0.505167 5.3:-3730094 None:intergenic
GGAATTCTATGGCGGGAAGA+AGG 0.510525 5.3:+3730298 MS.gene072765:intron
TTTATATGTGCATTGATGCC+AGG 0.514115 5.3:+3730353 MS.gene072765:CDS
TAGCACCATGATTGAGGTTG+TGG 0.516452 5.3:-3730569 None:intergenic
GTGATAATGTGAAGTTTGGT+TGG 0.518804 5.3:+3729099 MS.gene072765:CDS
CAGCTGCAGCCTCAGTTCCA+GGG 0.523289 5.3:-3729493 None:intergenic
AAACGCAGATCATAATTGTT+GGG 0.523983 5.3:+3729415 MS.gene072765:CDS
CATGTGGGTGCTATTGTTGG+AGG 0.529821 5.3:+3730958 MS.gene072765:CDS
CAAATATAGAGGTTCTTACT+CGG 0.533103 5.3:+3729853 MS.gene072765:CDS
GTTGGAGGATACTACGACGC+GGG 0.534176 5.3:+3729077 MS.gene072765:CDS
TAGAGCCTTTGCAAAATCAC+AGG 0.537879 5.3:+3730672 MS.gene072765:CDS
CATATCTCAACCTGCCAAAG+TGG 0.541021 5.3:+3730882 MS.gene072765:CDS
ATATCTCAACCTGCCAAAGT+GGG 0.542805 5.3:+3730883 MS.gene072765:CDS
TCCAAATCCTAATATTCATG+TGG 0.544345 5.3:+3730942 MS.gene072765:CDS
TTCGAATTCCCCTGGAACTG+AGG 0.548903 5.3:+3729484 MS.gene072765:CDS
ATATACCACAACCTCAATCA+TGG 0.550365 5.3:+3730564 MS.gene072765:CDS
CTTCGCAGTGCTATTCGTTG+GGG 0.551133 5.3:+3729206 MS.gene072765:CDS
TCAAGAAAATTGAAGAGTCT+CGG 0.553231 5.3:-3731100 None:intergenic
AATGAATAGCACCATGATTG+AGG 0.554404 5.3:-3730575 None:intergenic
CACTGCGAAGATAATCAAGC+TGG 0.565610 5.3:-3729193 None:intergenic
AAATATAGAGGTTCTTACTC+GGG 0.568349 5.3:+3729854 MS.gene072765:CDS
GCAAAGGCTCTAATTTCAGA+GGG 0.569361 5.3:-3730661 None:intergenic
TGTGATTGCTAGGTGGGAGA+TGG 0.571605 5.3:+3729392 MS.gene072765:intron
TTCTCAAGGAATTCTATGGC+GGG 0.576719 5.3:+3730291 MS.gene072765:intron
ACATCACAAGTAACCAAGGT+TGG 0.578483 5.3:+3730081 MS.gene072765:CDS
TTTCTCAAGGAATTCTATGG+CGG 0.583307 5.3:+3730290 MS.gene072765:intron
AGCTGCAGCCTCAGTTCCAG+GGG 0.585982 5.3:-3729492 None:intergenic
TTGGGAGCGTCCAGAAGACA+TGG 0.591746 5.3:+3729433 MS.gene072765:CDS
ACCAACCACGCTCTTTACAC+AGG 0.592498 5.3:+3729259 MS.gene072765:CDS
CTACAGATTAGAACAACTCC+AGG 0.598543 5.3:+3730386 MS.gene072765:CDS
CCAAATCCTAATATTCATGT+GGG 0.601860 5.3:+3730943 MS.gene072765:CDS
CAGGTTGAGATATGATTGAA+GGG 0.604313 5.3:-3730873 None:intergenic
ACCATGTATGACATCTTCAT+GGG 0.604739 5.3:-3730799 None:intergenic
ACGCTCAGGGAAGTTGCCTA+AGG 0.607352 5.3:+3728843 MS.gene072765:CDS
TCCCATGAAGATGTCATACA+TGG 0.607679 5.3:+3730798 MS.gene072765:CDS
GCAGCATTCATATAGGTGGT+AGG 0.611057 5.3:-3731036 None:intergenic
AGCTCAAACATTACTAACAC+AGG 0.615406 5.3:+3730148 MS.gene072765:CDS
AAAAGCAGCATTCATATAGG+TGG 0.619409 5.3:-3731040 None:intergenic
ACCTGTGTAAAGAGCGTGGT+TGG 0.623720 5.3:-3729260 None:intergenic
AATTGAAGCAGCAGAAAGAG+CGG 0.625859 5.3:-3729525 None:intergenic
TTAGCCAAGATTAAGACTGA+TGG 0.628467 5.3:+3730323 MS.gene072765:CDS
AGCAGTGTCTGAATTCAGCT+GGG 0.628902 5.3:+3730109 MS.gene072765:CDS
ATGAAGATGTCATACATGGT+GGG 0.629064 5.3:+3730802 MS.gene072765:CDS
AAAGGCTCTAATTTCAGAGG+GGG 0.637646 5.3:-3730659 None:intergenic
TGCATAGTCATAATTAGCTG+AGG 0.638379 5.3:-3728782 None:intergenic
CATGAAGATGTCATACATGG+TGG 0.650028 5.3:+3730801 MS.gene072765:CDS
TATCTCAACCTGCCAAAGTG+GGG 0.664840 5.3:+3730884 MS.gene072765:CDS
AAGCTCACCGTAAAAGACAT+CGG 0.669269 5.3:-3729122 None:intergenic
CATATTGATGGAATTCAGTG+TGG 0.674538 5.3:+3730622 MS.gene072765:CDS
TCACACCTGTGTAAAGAGCG+TGG 0.687393 5.3:-3729264 None:intergenic
ACGTACATCACAAGTAACCA+AGG 0.694288 5.3:+3730077 MS.gene072765:CDS
CAAAGGCTCTAATTTCAGAG+GGG 0.704501 5.3:-3730660 None:intergenic
CCAAAACAATTACATCACAG+AGG 0.744075 5.3:+3730841 MS.gene072765:CDS
GGTTGGAGGATACTACGACG+CGG 0.797840 5.3:+3729076 MS.gene072765:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATAAAAATCATATTGA+TGG + chr5.3:3730610-3730629 MS.gene072765:CDS 10.0%
!!! ATGATTTTTATTTAAGATTT+TGG - chr5.3:3730605-3730624 None:intergenic 10.0%
!! TAAAATGTATACATTATCAT+AGG - chr5.3:3729300-3729319 None:intergenic 15.0%
!!! AATGTTTTATTTTTTACAAG+AGG - chr5.3:3729928-3729947 None:intergenic 15.0%
!!! ATTTTAAATTGTGTATGAAA+AGG + chr5.3:3729995-3730014 MS.gene072765:intron 15.0%
!! TTTACTAAAAGAATACAGTT+TGG - chr5.3:3730214-3730233 None:intergenic 20.0%
!! TTTAGTAAACATTAGTTCAA+AGG + chr5.3:3730225-3730244 MS.gene072765:intron 20.0%
!! TTTCATAGATCATTCATTTA+TGG + chr5.3:3729967-3729986 MS.gene072765:intron 20.0%
!!! CAAAATTGTGGTTAAATTTT+AGG - chr5.3:3728948-3728967 None:intergenic 20.0%
!!! TATTATTTTGTTGTTAACTC+AGG + chr5.3:3730505-3730524 MS.gene072765:intron 20.0%
!!! TTCTAAAATATCAAACTCAT+TGG + chr5.3:3730718-3730737 MS.gene072765:intron 20.0%
! ATATGAAAGTGAGATTTCTT+CGG + chr5.3:3729166-3729185 MS.gene072765:CDS 25.0%
! ATGAAAAGGATGAACTATTA+TGG + chr5.3:3730009-3730028 MS.gene072765:intron 25.0%
! TGAAAAGGATGAACTATTAT+GGG + chr5.3:3730010-3730029 MS.gene072765:intron 25.0%
! TTTGGCTATATGGTAATTAA+TGG - chr5.3:3730196-3730215 None:intergenic 25.0%
!! AAATATTAGCTCTTGATCAA+TGG - chr5.3:3729674-3729693 None:intergenic 25.0%
!! TTGTATTGGTCATTAGAATT+TGG - chr5.3:3730985-3731004 None:intergenic 25.0%
!! TTTTCTTTCTTCTTTTCTCA+AGG + chr5.3:3730277-3730296 MS.gene072765:intron 25.0%
AAACGCAGATCATAATTGTT+GGG + chr5.3:3729415-3729434 MS.gene072765:CDS 30.0%
AAATATAGAGGTTCTTACTC+GGG + chr5.3:3729854-3729873 MS.gene072765:CDS 30.0%
AACAAAAGCAGCATTCATAT+AGG - chr5.3:3731046-3731065 None:intergenic 30.0%
AAGAATACAGTTTGGCTATA+TGG - chr5.3:3730206-3730225 None:intergenic 30.0%
ACTTGAATGTGACATTACAT+AGG + chr5.3:3730753-3730772 MS.gene072765:intron 30.0%
ATGTGTATTGTATGTGTCAT+TGG + chr5.3:3729042-3729061 MS.gene072765:intron 30.0%
CAAAATCTGTAAGCAATTCA+AGG + chr5.3:3729597-3729616 MS.gene072765:intron 30.0%
CAAATATAGAGGTTCTTACT+CGG + chr5.3:3729853-3729872 MS.gene072765:CDS 30.0%
CACAGGTATATATACTAATC+TGG + chr5.3:3730689-3730708 MS.gene072765:intron 30.0%
CATGAATCATGATGATCAAT+TGG + chr5.3:3729639-3729658 MS.gene072765:intron 30.0%
CCAAATCCTAATATTCATGT+GGG + chr5.3:3730943-3730962 MS.gene072765:CDS 30.0%
TAGATGAAGAGAAATAGTTC+TGG - chr5.3:3730921-3730940 None:intergenic 30.0%
TCAAGAAAATTGAAGAGTCT+CGG - chr5.3:3731103-3731122 None:intergenic 30.0%
TCAATCTTGTAAGTTGTTCT+TGG - chr5.3:3729464-3729483 None:intergenic 30.0%
TCCAAATCCTAATATTCATG+TGG + chr5.3:3730942-3730961 MS.gene072765:CDS 30.0%
TTCAATCAACGTACATATGA+TGG + chr5.3:3730465-3730484 MS.gene072765:intron 30.0%
TTGTCCTTCAAAAAACATGA+TGG - chr5.3:3728824-3728843 None:intergenic 30.0%
! ATATACCTGTGATTTTGCAA+AGG - chr5.3:3730680-3730699 None:intergenic 30.0%
! GATTTTGCTGACAAATATAG+AGG + chr5.3:3729842-3729861 MS.gene072765:CDS 30.0%
! TCTTTTCTCAAGGAATTCTA+TGG + chr5.3:3730287-3730306 MS.gene072765:intron 30.0%
! TTTAACCACAATTTTGCACT+AGG + chr5.3:3728952-3728971 MS.gene072765:intron 30.0%
!! AATTGCTTACAGATTTTGAC+TGG - chr5.3:3729595-3729614 None:intergenic 30.0%
!!! AAAGCCATCATGTTTTTTGA+AGG + chr5.3:3728817-3728836 MS.gene072765:CDS 30.0%
!!! ATTTTGTTGTTAACTCAGGT+GGG + chr5.3:3730509-3730528 MS.gene072765:intron 30.0%
!!! TATATGAATGCTGCTTTTGT+TGG + chr5.3:3731045-3731064 MS.gene072765:CDS 30.0%
!!! TATTTTGTTGTTAACTCAGG+TGG + chr5.3:3730508-3730527 MS.gene072765:intron 30.0%
AAAAGCAGCATTCATATAGG+TGG - chr5.3:3731043-3731062 None:intergenic 35.0%
AATGAATAGCACCATGATTG+AGG - chr5.3:3730578-3730597 None:intergenic 35.0%
ACCATGTATGACATCTTCAT+GGG - chr5.3:3730802-3730821 None:intergenic 35.0%
AGCTCAAACATTACTAACAC+AGG + chr5.3:3730148-3730167 MS.gene072765:CDS 35.0%
AGCTGGGATAACAAATTTGT+TGG + chr5.3:3730125-3730144 MS.gene072765:CDS 35.0%
ATAGCACCCACATGAATATT+AGG - chr5.3:3730952-3730971 None:intergenic 35.0%
ATATACCACAACCTCAATCA+TGG + chr5.3:3730564-3730583 MS.gene072765:CDS 35.0%
ATGAAGATGTCATACATGGT+GGG + chr5.3:3730802-3730821 MS.gene072765:CDS 35.0%
CAAATATCGTTGTGATTGCT+AGG + chr5.3:3729382-3729401 MS.gene072765:intron 35.0%
CAGATCTTGCATCATTGTAT+TGG - chr5.3:3730999-3731018 None:intergenic 35.0%
CATATTGATGGAATTCAGTG+TGG + chr5.3:3730622-3730641 MS.gene072765:CDS 35.0%
CCAAAACAATTACATCACAG+AGG + chr5.3:3730841-3730860 MS.gene072765:CDS 35.0%
CCCACATGAATATTAGGATT+TGG - chr5.3:3730946-3730965 None:intergenic 35.0%
GAAACGCAGATCATAATTGT+TGG + chr5.3:3729414-3729433 MS.gene072765:CDS 35.0%
GCAATCATTACATATGTACC+TGG - chr5.3:3730407-3730426 None:intergenic 35.0%
TGAATGTGACATTACATAGG+TGG + chr5.3:3730756-3730775 MS.gene072765:intron 35.0%
TGCATAGTCATAATTAGCTG+AGG - chr5.3:3728785-3728804 None:intergenic 35.0%
TTAGCCAAGATTAAGACTGA+TGG + chr5.3:3730323-3730342 MS.gene072765:CDS 35.0%
TTCACCATCAGTCTTAATCT+TGG - chr5.3:3730330-3730349 None:intergenic 35.0%
TTGAGATATGATTGAAGGGA+TGG - chr5.3:3730872-3730891 None:intergenic 35.0%
TTTCTCAAGGAATTCTATGG+CGG + chr5.3:3730290-3730309 MS.gene072765:intron 35.0%
! CAGGTTGAGATATGATTGAA+GGG - chr5.3:3730876-3730895 None:intergenic 35.0%
! CCTCTGTGATGTAATTGTTT+TGG - chr5.3:3730844-3730863 None:intergenic 35.0%
! GTGATAATGTGAAGTTTGGT+TGG + chr5.3:3729099-3729118 MS.gene072765:CDS 35.0%
! TTTATATGTGCATTGATGCC+AGG + chr5.3:3730353-3730372 MS.gene072765:CDS 35.0%
!! ACATAGGTGGACTACATATT+AGG + chr5.3:3730769-3730788 MS.gene072765:intron 35.0%
!!! TTTTGACTGGCTTAATAGCT+TGG - chr5.3:3729582-3729601 None:intergenic 35.0%
AAGCTCACCGTAAAAGACAT+CGG - chr5.3:3729125-3729144 None:intergenic 40.0%
AATTGAAGCAGCAGAAAGAG+CGG - chr5.3:3729528-3729547 None:intergenic 40.0%
AATTTCAGAGGGGGAGAAAT+GGG - chr5.3:3730653-3730672 None:intergenic 40.0%
ACATCACAAGTAACCAAGGT+TGG + chr5.3:3730081-3730100 MS.gene072765:CDS 40.0%
ACGTACATCACAAGTAACCA+AGG + chr5.3:3730077-3730096 MS.gene072765:CDS 40.0%
ATATCGTTGTGATTGCTAGG+TGG + chr5.3:3729385-3729404 MS.gene072765:intron 40.0%
ATATCTCAACCTGCCAAAGT+GGG + chr5.3:3730883-3730902 MS.gene072765:CDS 40.0%
CACCATGTATGACATCTTCA+TGG - chr5.3:3730803-3730822 None:intergenic 40.0%
CAGAGTACGAGCAGTAAAAT+GGG - chr5.3:3729886-3729905 None:intergenic 40.0%
CATGAAGATGTCATACATGG+TGG + chr5.3:3730801-3730820 MS.gene072765:CDS 40.0%
CTACAGATTAGAACAACTCC+AGG + chr5.3:3730386-3730405 MS.gene072765:CDS 40.0%
TAATTTCAGAGGGGGAGAAA+TGG - chr5.3:3730654-3730673 None:intergenic 40.0%
TAGAGCCTTTGCAAAATCAC+AGG + chr5.3:3730672-3730691 MS.gene072765:CDS 40.0%
TATCGTTGTGATTGCTAGGT+GGG + chr5.3:3729386-3729405 MS.gene072765:intron 40.0%
TCCCATGAAGATGTCATACA+TGG + chr5.3:3730798-3730817 MS.gene072765:CDS 40.0%
TTCTCAAGGAATTCTATGGC+GGG + chr5.3:3730291-3730310 MS.gene072765:intron 40.0%
TTGAGCCTAGTGCAAAATTG+TGG - chr5.3:3728960-3728979 None:intergenic 40.0%
! AAAGGCTCTAATTTCAGAGG+GGG - chr5.3:3730662-3730681 None:intergenic 40.0%
! AACTATTATGGGCTGCTTCT+TGG + chr5.3:3730021-3730040 MS.gene072765:intron 40.0%
! ATGTCATACATGGTGGGATA+TGG + chr5.3:3730808-3730827 MS.gene072765:CDS 40.0%
! CAAAGGCTCTAATTTCAGAG+GGG - chr5.3:3730663-3730682 None:intergenic 40.0%
! GCAAAGGCTCTAATTTCAGA+GGG - chr5.3:3730664-3730683 None:intergenic 40.0%
! GCAGGTTGAGATATGATTGA+AGG - chr5.3:3730877-3730896 None:intergenic 40.0%
! GTATTGTATGTGTCATTGGC+AGG + chr5.3:3729046-3729065 MS.gene072765:intron 40.0%
! TGCAAAGGCTCTAATTTCAG+AGG - chr5.3:3730665-3730684 None:intergenic 40.0%
! TGTCATACATGGTGGGATAT+GGG + chr5.3:3730809-3730828 MS.gene072765:CDS 40.0%
!! AAGATTGATTCGAATTCCCC+TGG + chr5.3:3729476-3729495 MS.gene072765:CDS 40.0%
!! ATTCATGTGGGTGCTATTGT+TGG + chr5.3:3730955-3730974 MS.gene072765:CDS 40.0%
!! TTTTTGAAGGACAACGCTCA+GGG + chr5.3:3728830-3728849 MS.gene072765:CDS 40.0%
!!! TTTTTTGAAGGACAACGCTC+AGG + chr5.3:3728829-3728848 MS.gene072765:CDS 40.0%
AAGCAGTGTCTGAATTCAGC+TGG + chr5.3:3730108-3730127 MS.gene072765:CDS 45.0%
AGCAGTGTCTGAATTCAGCT+GGG + chr5.3:3730109-3730128 MS.gene072765:CDS 45.0%
ATCTGTAGAGAGCTACTTCC+TGG - chr5.3:3730374-3730393 None:intergenic 45.0%
CACTGCGAAGATAATCAAGC+TGG - chr5.3:3729196-3729215 None:intergenic 45.0%
CACTTCACTCTTTGGTCCTT+AGG - chr5.3:3728862-3728881 None:intergenic 45.0%
CATATCTCAACCTGCCAAAG+TGG + chr5.3:3730882-3730901 MS.gene072765:CDS 45.0%
CCAGAGTACGAGCAGTAAAA+TGG - chr5.3:3729887-3729906 None:intergenic 45.0%
CTAAGGACCAAAGAGTGAAG+TGG + chr5.3:3728860-3728879 MS.gene072765:intron 45.0%
CTGCTCGTACTCTGGTTATT+AGG + chr5.3:3729892-3729911 MS.gene072765:intron 45.0%
CTTGGCTATACAAGGCTAGT+GGG + chr5.3:3730039-3730058 MS.gene072765:intron 45.0%
GCAGCATTCATATAGGTGGT+AGG - chr5.3:3731039-3731058 None:intergenic 45.0%
TAGCACCATGATTGAGGTTG+TGG - chr5.3:3730572-3730591 None:intergenic 45.0%
TATCTCAACCTGCCAAAGTG+GGG + chr5.3:3730884-3730903 MS.gene072765:CDS 45.0%
TCTTGGCTATACAAGGCTAG+TGG + chr5.3:3730038-3730057 MS.gene072765:intron 45.0%
TTGGCTATACAAGGCTAGTG+GGG + chr5.3:3730040-3730059 MS.gene072765:intron 45.0%
! ATCTTCGCAGTGCTATTCGT+TGG + chr5.3:3729204-3729223 MS.gene072765:CDS 45.0%
! CCATTTTACTGCTCGTACTC+TGG + chr5.3:3729884-3729903 MS.gene072765:intron 45.0%
! CTTGGTGTATCCATGTCTTC+TGG - chr5.3:3729446-3729465 None:intergenic 45.0%
! GCGGGTGATAATGTGAAGTT+TGG + chr5.3:3729095-3729114 MS.gene072765:CDS 45.0%
! TCTTCGCAGTGCTATTCGTT+GGG + chr5.3:3729205-3729224 MS.gene072765:CDS 45.0%
! TTGTATGTGTCATTGGCAGG+TGG + chr5.3:3729049-3729068 MS.gene072765:intron 45.0%
!! TGGTTGGCCGATGTCTTTTA+CGG + chr5.3:3729115-3729134 MS.gene072765:CDS 45.0%
!! TTACGGTGAGCTTGTTGAGT+TGG + chr5.3:3729132-3729151 MS.gene072765:CDS 45.0%
!! TTAATTATATAAAATCAAAA+TGG - chr5.3:3729697-3729716 None:intergenic 5.0%
!!! ATTTTATATAATTAAACTTA+AGG + chr5.3:3729702-3729721 MS.gene072765:intron 5.0%
!!! TTATATTTTTTTTTTTTGAA+TGG - chr5.3:3729728-3729747 None:intergenic 5.0%
ACACTGCTTGACTCCAACCT+TGG - chr5.3:3730097-3730116 None:intergenic 50.0%
ACCAACCACGCTCTTTACAC+AGG + chr5.3:3729259-3729278 MS.gene072765:CDS 50.0%
AGGACCAAAGAGTGAAGTGG+AGG + chr5.3:3728863-3728882 MS.gene072765:intron 50.0%
GACCAAAGAGTGAAGTGGAG+GGG + chr5.3:3728865-3728884 MS.gene072765:intron 50.0%
GGACCAAAGAGTGAAGTGGA+GGG + chr5.3:3728864-3728883 MS.gene072765:intron 50.0%
GGCTGCTTCTTGGCTATACA+AGG + chr5.3:3730031-3730050 MS.gene072765:intron 50.0%
GGTTCTGTTGCAGCTTTGCT+TGG + chr5.3:3731066-3731085 MS.gene072765:CDS 50.0%
TCACACCTGTGTAAAGAGCG+TGG - chr5.3:3729267-3729286 None:intergenic 50.0%
TGTGATTGCTAGGTGGGAGA+TGG + chr5.3:3729392-3729411 MS.gene072765:intron 50.0%
TTCGAATTCCCCTGGAACTG+AGG + chr5.3:3729484-3729503 MS.gene072765:CDS 50.0%
! ACCTGTGTAAAGAGCGTGGT+TGG - chr5.3:3729263-3729282 None:intergenic 50.0%
! CATTGGCAGGTGGATTTGGT+TGG + chr5.3:3729059-3729078 MS.gene072765:intron 50.0%
! CTTCGCAGTGCTATTCGTTG+GGG + chr5.3:3729206-3729225 MS.gene072765:CDS 50.0%
! GGAATTCTATGGCGGGAAGA+AGG + chr5.3:3730298-3730317 MS.gene072765:intron 50.0%
! GTGTCATTGGCAGGTGGATT+TGG + chr5.3:3729055-3729074 MS.gene072765:intron 50.0%
!! CATGTGGGTGCTATTGTTGG+AGG + chr5.3:3730958-3730977 MS.gene072765:CDS 50.0%
ACGCTCAGGGAAGTTGCCTA+AGG + chr5.3:3728843-3728862 MS.gene072765:CDS 55.0%
AGTGAAGTGGAGGGGAGACT+CGG + chr5.3:3728873-3728892 MS.gene072765:intron 55.0%
CTCCCCTCCACTTCACTCTT+TGG - chr5.3:3728870-3728889 None:intergenic 55.0%
GCCAAAGTGGGGTGCAATGA+TGG + chr5.3:3730895-3730914 MS.gene072765:CDS 55.0%
GCCATCATTGCACCCCACTT+TGG - chr5.3:3730899-3730918 None:intergenic 55.0%
GGTTGGAGGATACTACGACG+CGG + chr5.3:3729076-3729095 MS.gene072765:CDS 55.0%
GTTGGAGGATACTACGACGC+GGG + chr5.3:3729077-3729096 MS.gene072765:CDS 55.0%
TCATTGCACCCCACTTTGGC+AGG - chr5.3:3730895-3730914 None:intergenic 55.0%
! TGGCAGGTGGATTTGGTTGG+AGG + chr5.3:3729062-3729081 MS.gene072765:intron 55.0%
!! TTGGGAGCGTCCAGAAGACA+TGG + chr5.3:3729433-3729452 MS.gene072765:CDS 55.0%
AGCTGCAGCCTCAGTTCCAG+GGG - chr5.3:3729495-3729514 None:intergenic 60.0%
CAGCTGCAGCCTCAGTTCCA+GGG - chr5.3:3729496-3729515 None:intergenic 60.0%
GGAGACTCGGCTCTCTCTGA+TGG + chr5.3:3728886-3728905 MS.gene072765:intron 60.0%
TCAGCTGCAGCCTCAGTTCC+AGG - chr5.3:3729497-3729516 None:intergenic 60.0%
TGGAACTGAGGCTGCAGCTG+AGG + chr5.3:3729496-3729515 MS.gene072765:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 3728754 3731122 3728754 ID=MS.gene072765
chr5.3 mRNA 3728754 3731122 3728754 ID=MS.gene072765.t1;Parent=MS.gene072765
chr5.3 exon 3728754 3728873 3728754 ID=MS.gene072765.t1.exon1;Parent=MS.gene072765.t1
chr5.3 CDS 3728754 3728873 3728754 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3729068 3729280 3729068 ID=MS.gene072765.t1.exon2;Parent=MS.gene072765.t1
chr5.3 CDS 3729068 3729280 3729068 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3729404 3729604 3729404 ID=MS.gene072765.t1.exon3;Parent=MS.gene072765.t1
chr5.3 CDS 3729404 3729604 3729404 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3729836 3729897 3729836 ID=MS.gene072765.t1.exon4;Parent=MS.gene072765.t1
chr5.3 CDS 3729836 3729897 3729836 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3730058 3730169 3730058 ID=MS.gene072765.t1.exon5;Parent=MS.gene072765.t1
chr5.3 CDS 3730058 3730169 3730058 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3730299 3730407 3730299 ID=MS.gene072765.t1.exon6;Parent=MS.gene072765.t1
chr5.3 CDS 3730299 3730407 3730299 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3730527 3730693 3730527 ID=MS.gene072765.t1.exon7;Parent=MS.gene072765.t1
chr5.3 CDS 3730527 3730693 3730527 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
chr5.3 exon 3730775 3731122 3730775 ID=MS.gene072765.t1.exon8;Parent=MS.gene072765.t1
chr5.3 CDS 3730775 3731122 3730775 ID=cds.MS.gene072765.t1;Parent=MS.gene072765.t1
Gene Sequence

>MS.gene072765

ATGGATGATGAATTCATTAATACAAATTCCTCAGCTAATTATGACTATGCAGATGCTCTTGACAAAGCCATCATGTTTTTTGAAGGACAACGCTCAGGGAAGTTGCCTAAGGACCAAAGAGTGAAGTGGAGGGGAGACTCGGCTCTCTCTGATGGCAAGACTCAAAATGTATACAAATATACACGACATGACCTAAAATTTAACCACAATTTTGCACTAGGCTCAATATAACTTTATACAATGCTCTATATATGTCTGTTTTTTGATATGAATTCTTTATGAACAATCATGTGTATTGTATGTGTCATTGGCAGGTGGATTTGGTTGGAGGATACTACGACGCGGGTGATAATGTGAAGTTTGGTTGGCCGATGTCTTTTACGGTGAGCTTGTTGAGTTGGACTGCTGTTGAATATGAAAGTGAGATTTCTTCGGTGAACCAGCTTGATTATCTTCGCAGTGCTATTCGTTGGGGTGCAGATTTCATACTTAAAGCTCACACTTCACCAACCACGCTCTTTACACAGGTGTGATTCTATATATCCTATGATAATGTATACATTTTAGATAATATAATGTACACATAATGTGTGTGTGATTTGTGTTGCATGTTGATTAGTTCTATAATCAAATATCGTTGTGATTGCTAGGTGGGAGATGGAAACGCAGATCATAATTGTTGGGAGCGTCCAGAAGACATGGATACACCAAGAACAACTTACAAGATTGATTCGAATTCCCCTGGAACTGAGGCTGCAGCTGAGGCTGCTGCCGCTCTTTCTGCTGCTTCAATTGTCTTTAAGAAAACAGATATCAATTATTCATCCAAGCTATTAAGCCAGTCAAAATCTGTAAGCAATTCAAGGAACATGTTTTATTCTCATTCATGAATCATGATGATCAATTGGCTCTATAATCCATTGATCAAGAGCTAATATTTCCATTTTGATTTTATATAATTAAACTTAAGGCCATTCAAAAAAAAAAAATATAATTAAACTTAAGAAACAAGTGAAATTTGCATTAGAGTGTTTATGTATTTAATTTAACAAAATTTACTAATAATTAAATATGTTTTGCAGTTGTTTGATTTTGCTGACAAATATAGAGGTTCTTACTCGGGTTCTTGCCCATTTTACTGCTCGTACTCTGGTTATTAGGTAAGAAATTACCTCTTGTAAAAAATAAAACATTTAAAGACTAATAATTATTTTTTCATAGATCATTCATTTATGGTTGTTATTTTAAATTGTGTATGAAAAGGATGAACTATTATGGGCTGCTTCTTGGCTATACAAGGCTAGTGGGGAAAGCAAGTACTTGACGTACATCACAAGTAACCAAGGTTGGAGTCAAGCAGTGTCTGAATTCAGCTGGGATAACAAATTTGTTGGAGCTCAAACATTACTAACACAGGTATTTACATTTATAGCTAATTACCATTAATTACCATATAGCCAAACTGTATTCTTTTAGTAAACATTAGTTCAAAGGAAAAAACTTAACTCATTAATTCTAATTGTTTTTCTTTCTTCTTTTCTCAAGGAATTCTATGGCGGGAAGAAGGAGTTAGCCAAGATTAAGACTGATGGTGAATCATTTATATGTGCATTGATGCCAGGAAGTAGCTCTCTACAGATTAGAACAACTCCAGGTACATATGTAATGATTGCAAAGTAATATTACATATAACACAAATAGTCAATCTTATTTCAATCAACGTACATATGATGGAATGAGCTCAGTTAGTGTATTATTTTGTTGTTAACTCAGGTGGGCTTCTGTATATTAGAGATAGTAGTAATTTTCAATATACCACAACCTCAATCATGGTGCTATTCATTTTCTCCAAAATCTTAAATAAAAATCATATTGATGGAATTCAGTGTGGCTCTGCCCATTTCTCCCCCTCTGAAATTAGAGCCTTTGCAAAATCACAGGTATATATACTAATCTGGTTTTGCTTCTAAAATATCAAACTCATTGGTGCATAACTAAAACTTGAATGTGACATTACATAGGTGGACTACATATTAGGAAACAATCCCATGAAGATGTCATACATGGTGGGATATGGGAGTAAATATCCAAAACAATTACATCACAGAGGTTCTTCCATCCCTTCAATCATATCTCAACCTGCCAAAGTGGGGTGCAATGATGGCCAGAACTATTTCTCTTCATCTAATCCAAATCCTAATATTCATGTGGGTGCTATTGTTGGAGGACCAAATTCTAATGACCAATACAATGATGCAAGATCTGATTACTCTCATGCAGAACCTACCACCTATATGAATGCTGCTTTTGTTGGTTCTGTTGCAGCTTTGCTTGGTGAAACTAACACCGAGACTCTTCAATTTTCTTGA
Protein sequence

>MS.gene072765.t1

MDDEFINTNSSANYDYADALDKAIMFFEGQRSGKLPKDQRVDLVGGYYDAGDNVKFGWPMSFTVSLLSWTAVEYESEISSVNQLDYLRSAIRWGADFILKAHTSPTTLFTQVGDGNADHNCWERPEDMDTPRTTYKIDSNSPGTEAAAEAAAALSAASIVFKKTDINYSSKLLSQSKSLFDFADKYRGSYSGSCPFYCSGESKYLTYITSNQGWSQAVSEFSWDNKFVGAQTLLTQEFYGGKKELAKIKTDGESFICALMPGSSSLQIRTTPGGLLYIRDSSNFQYTTTSIMVLFIFSKILNKNHIDGIQCGSAHFSPSEIRAFAKSQVDYILGNNPMKMSYMVGYGSKYPKQLHHRGSSIPSIISQPAKVGCNDGQNYFSSSNPNPNIHVGAIVGGPNSNDQYNDARSDYSHAEPTTYMNAAFVGSVAALLGETNTETLQFS