AlfalfaGEDB | MS.gene20757

M. sativa cultivar XinJiangDaYe / MS.gene20757

KEGG: K01179
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00759.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20757.t1	RHN81090.1	99.4	488	3	0	18	505	16	503	1.90E-290	1007.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20757.t1	O81416	80.2	470	93	0	36	505	47	516	2.0e-235	816.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20757.t1	A0A396JS05	99.4	488	3	0	18	505	16	503	1.4e-290	1007.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052308	MS.gene20757	0.817334	3.48E-52	-1.69E-46
MS.gene052367	MS.gene20757	0.811564	6.57E-51	-1.69E-46
MS.gene055308	MS.gene20757	0.801404	9.15E-49	-1.69E-46
MS.gene055578	MS.gene20757	0.816029	6.83E-52	-1.69E-46
MS.gene055957	MS.gene20757	0.826261	2.99E-54	-1.69E-46
MS.gene056041	MS.gene20757	0.835052	2.10E-56	-1.69E-46
MS.gene057458	MS.gene20757	0.811216	7.82E-51	-1.69E-46
MS.gene060473	MS.gene20757	0.826115	3.24E-54	-1.69E-46
MS.gene060565	MS.gene20757	0.814333	1.63E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene20757	MS.gene42272	PPI
MS.gene014436	MS.gene20757	PPI
MS.gene024647	MS.gene20757	PPI
MS.gene20757	MS.gene057924	PPI
MS.gene20757	MS.gene21635	PPI
MS.gene20757	MS.gene25810	PPI
MS.gene68770	MS.gene20757	PPI
MS.gene20757	MS.gene88560	PPI
MS.gene20757	MS.gene69412	PPI
MS.gene20757	MS.gene015691	PPI
MS.gene29940	MS.gene20757	PPI
MS.gene20757	MS.gene020482	PPI
MS.gene20757	MS.gene024650	PPI
MS.gene72272	MS.gene20757	PPI
MS.gene22342	MS.gene20757	PPI
MS.gene20757	MS.gene057880	PPI
MS.gene20757	MS.gene32754	PPI
MS.gene68175	MS.gene20757	PPI
MS.gene20757	MS.gene81918	PPI
MS.gene072765	MS.gene20757	PPI
MS.gene20757	MS.gene063122	PPI
MS.gene20757	MS.gene51810	PPI
MS.gene20757	MS.gene95919	PPI
MS.gene020485	MS.gene20757	PPI
MS.gene20757	MS.gene02701	PPI
MS.gene20757	MS.gene057923	PPI
MS.gene20757	MS.gene044195	PPI
MS.gene20757	MS.gene88559	PPI
MS.gene20757	MS.gene95918	PPI
MS.gene20757	MS.gene026233	PPI
MS.gene20757	MS.gene81610	PPI
MS.gene20757	MS.gene96428	PPI
MS.gene20757	MS.gene81916	PPI
MS.gene020483	MS.gene20757	PPI
MS.gene072763	MS.gene20757	PPI
MS.gene20757	MS.gene014234	PPI
MS.gene20757	MS.gene95200	PPI
MS.gene20757	MS.gene016750	PPI
MS.gene20757	MS.gene50877	PPI
MS.gene014960	MS.gene20757	PPI
MS.gene20757	MS.gene063734	PPI
MS.gene20757	MS.gene70730	PPI
MS.gene20757	MS.gene21633	PPI
MS.gene20757	MS.gene026232	PPI
MS.gene20757	MS.gene42640	PPI
MS.gene070498	MS.gene20757	PPI
MS.gene20757	MS.gene064746	PPI
MS.gene019323	MS.gene20757	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20757.t1	MTR_1g088190	94.466	506	6	3	1	505	1	485	0.0	978
MS.gene20757.t1	MTR_1g088190	91.813	342	6	3	1	341	1	321	0.0	641
MS.gene20757.t1	MTR_3g080810	63.578	464	168	1	37	499	31	494	0.0	624
MS.gene20757.t1	MTR_8g104820	62.366	465	172	2	38	499	27	491	0.0	607
MS.gene20757.t1	MTR_3g117500	59.740	462	183	2	40	498	23	484	0.0	580
MS.gene20757.t1	MTR_3g010330	59.870	461	179	3	41	499	25	481	0.0	563
MS.gene20757.t1	MTR_4g035930	56.332	458	195	2	41	498	15	467	0.0	536
MS.gene20757.t1	MTR_4g035955	56.332	458	195	2	41	498	14	466	0.0	534
MS.gene20757.t1	MTR_4g035975	57.143	469	186	6	40	498	26	489	0.0	527
MS.gene20757.t1	MTR_5g010000	52.903	465	206	6	38	498	37	492	5.04e-172	494
MS.gene20757.t1	MTR_8g099410	52.008	473	215	7	40	503	27	496	2.86e-169	488
MS.gene20757.t1	MTR_5g009970	51.404	463	212	6	40	498	40	493	5.86e-168	484
MS.gene20757.t1	MTR_8g099410	52.008	473	215	7	40	503	27	496	6.38e-168	488
MS.gene20757.t1	MTR_3g080190	49.080	489	227	7	29	496	16	503	2.29e-161	468
MS.gene20757.t1	MTR_5g085730	46.586	498	242	8	13	500	15	498	2.07e-160	465
MS.gene20757.t1	MTR_5g093590	45.759	507	249	7	2	498	10	500	2.48e-154	449
MS.gene20757.t1	MTR_1g037340	48.708	503	231	10	3	499	1	482	1.78e-150	439
MS.gene20757.t1	MTR_3g105230	46.563	451	227	6	41	488	30	469	6.19e-147	430
MS.gene20757.t1	MTR_4g074960	46.835	474	241	7	41	505	28	499	6.16e-145	429
MS.gene20757.t1	MTR_5g009970	50.000	422	196	7	82	498	8	419	7.82e-145	422
MS.gene20757.t1	MTR_3g023740	46.537	462	233	6	41	499	30	480	3.75e-142	417
MS.gene20757.t1	MTR_5g009970	48.507	402	192	7	102	498	1	392	3.54e-131	387
MS.gene20757.t1	MTR_3g113720	41.474	475	260	9	35	498	51	518	1.80e-119	361
MS.gene20757.t1	MTR_8g075990	43.697	476	250	9	35	499	57	525	5.25e-117	355
MS.gene20757.t1	MTR_5g009970	50.000	334	154	6	168	498	1	324	1.06e-105	319
MS.gene20757.t1	MTR_7g108925	86.503	163	22	0	343	505	43	205	1.83e-101	304
MS.gene20757.t1	MTR_2g028480	41.412	524	261	17	8	499	81	590	3.29e-99	311
MS.gene20757.t1	MTR_3g082440	38.015	534	279	19	6	503	79	596	2.74e-91	291
MS.gene20757.t1	MTR_3g090540	35.038	528	287	18	9	498	74	583	4.37e-88	282
MS.gene20757.t1	MTR_0393s0050	61.429	140	53	1	343	481	20	159	9.66e-57	187
MS.gene20757.t1	MTR_1g037320	48.148	162	80	2	102	261	1	160	7.97e-35	132
MS.gene20757.t1	MTR_1g037320	45.714	140	71	3	361	496	151	289	5.13e-30	119
MS.gene20757.t1	MTR_7g108920	67.742	93	29	1	249	341	86	177	5.13e-32	121
MS.gene20757.t1	MTR_4g128810	36.842	152	90	3	350	499	187	334	1.85e-24	105

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20757.t1	AT4G02290	76.436	505	117	1	1	505	14	516	0.0	806
MS.gene20757.t1	AT1G02800	74.354	503	125	2	1	501	1	501	0.0	766
MS.gene20757.t1	AT4G02290	78.713	404	86	0	102	505	1	404	0.0	676
MS.gene20757.t1	AT1G70710	61.720	465	177	1	38	501	25	489	0.0	599
MS.gene20757.t1	AT1G23210	60.475	463	183	0	37	499	24	486	0.0	591
MS.gene20757.t1	AT1G22880	57.287	494	193	4	9	501	5	481	0.0	566
MS.gene20757.t1	AT1G71380	55.870	494	202	4	7	499	1	479	0.0	561
MS.gene20757.t1	AT4G39010	57.906	468	193	3	40	503	28	495	0.0	560
MS.gene20757.t1	AT4G39000	53.205	468	215	3	35	498	19	486	2.40e-179	513
MS.gene20757.t1	AT4G23560	53.548	465	204	5	42	503	24	479	9.00e-170	488
MS.gene20757.t1	AT1G64390	51.907	472	215	7	37	499	21	489	9.50e-170	493
MS.gene20757.t1	AT4G38990	51.163	473	222	5	35	498	17	489	7.48e-168	484
MS.gene20757.t1	AT4G09740	52.802	464	207	5	41	501	23	477	5.68e-167	481
MS.gene20757.t1	AT1G22880	56.858	401	168	2	102	501	1	397	1.22e-165	474
MS.gene20757.t1	AT4G11050	50.964	467	219	6	40	498	25	489	2.14e-165	479
MS.gene20757.t1	AT4G11050	50.964	467	219	6	40	498	25	489	6.32e-165	481
MS.gene20757.t1	AT4G11050	50.964	467	219	6	40	498	25	489	8.95e-165	481
MS.gene20757.t1	AT2G32990	49.095	497	237	7	9	496	13	502	2.56e-164	476
MS.gene20757.t1	AT2G44540	49.784	462	220	7	41	496	33	488	1.01e-150	440
MS.gene20757.t1	AT2G44570	49.680	469	223	8	38	499	30	492	9.81e-149	435
MS.gene20757.t1	AT2G44550	49.353	464	222	8	41	498	33	489	1.13e-148	434
MS.gene20757.t1	AT4G39010	56.944	360	151	3	40	395	28	387	1.49e-148	431
MS.gene20757.t1	AT3G43860	48.701	462	223	6	41	499	34	484	2.29e-147	431
MS.gene20757.t1	AT3G43860	48.701	462	223	6	41	499	34	484	2.29e-147	431
MS.gene20757.t1	AT2G44560	49.679	467	223	7	39	499	31	491	5.12e-146	428
MS.gene20757.t1	AT1G48930	46.908	469	231	7	40	496	29	491	2.03e-133	400
MS.gene20757.t1	AT1G19940	45.652	460	236	7	41	493	48	500	3.71e-130	388
MS.gene20757.t1	AT1G19940	45.652	460	236	7	41	493	48	500	3.71e-130	388
MS.gene20757.t1	AT1G75680	47.033	455	227	7	42	489	58	505	1.90e-129	387
MS.gene20757.t1	AT1G22880	56.647	346	132	4	9	353	5	333	1.17e-123	365
MS.gene20757.t1	AT2G44570	47.368	380	188	7	38	411	30	403	6.35e-105	320
MS.gene20757.t1	AT4G38990	49.118	340	148	3	35	372	17	333	3.99e-104	315
MS.gene20757.t1	AT5G49720	40.655	519	260	18	14	499	84	587	7.27e-93	295
MS.gene20757.t1	AT1G19940	44.628	363	191	6	41	396	48	407	9.43e-93	289
MS.gene20757.t1	AT4G24260	40.000	520	269	16	12	499	79	587	1.08e-90	290
MS.gene20757.t1	AT1G65610	37.148	533	279	20	6	504	86	596	8.49e-88	282
MS.gene20757.t1	AT4G24260	38.918	388	198	15	41	396	108	488	1.28e-53	188
MS.gene20757.t1	AT4G24260	40.058	347	174	12	12	329	79	420	4.81e-52	183

Find 147 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCCCTTCTCACTAAAATTT+TGG	0.161476	1.2:+65978605	None:intergenic
ACCAGAGTAATCACAGTAAA+AGG	0.170221	1.2:+65978422	None:intergenic
CATTAACTTGAATGTAATTT+AGG	0.180687	1.2:+65978243	None:intergenic
ATGTGATTTGGTCAAGTATT+TGG	0.246836	1.2:+65977739	None:intergenic
GCTGAACATTGGATCTTTCC+TGG	0.246957	1.2:+65977441	None:intergenic
CTGTGATTACTCTGGTTATC+AGG	0.254164	1.2:-65978415	MS.gene20757:intron
CTAAATTACATTCAAGTTAA+TGG	0.258488	1.2:-65978242	MS.gene20757:CDS
CTTGGTGTGTCCATGTCTTC+TGG	0.269068	1.2:+65978738	None:intergenic
TCAATCTTGAAAACACTTCT+TGG	0.276130	1.2:+65978720	None:intergenic
TTCGTTTGCTCTTTAATTCC+TGG	0.293478	1.2:-65977961	MS.gene20757:CDS
CTACTAGAATGTTGGGATTA+GGG	0.293793	1.2:+65977391	None:intergenic
GCTAAGGTTCTGAGTCTCTT+TGG	0.294218	1.2:+65977683	None:intergenic
GAGTCTCTTTGGTGTTACTA+TGG	0.297437	1.2:+65977694	None:intergenic
AAAACTAACCTGAACATAAA+TGG	0.298563	1.2:+65978883	None:intergenic
TTACTACCACTATGCTTTCA+TGG	0.301130	1.2:-65979012	MS.gene20757:CDS
TCATAATCTGACCGTTTATC+TGG	0.305225	1.2:+65977330	None:intergenic
AAGGTAGCTTCCCTGATCTT+TGG	0.305903	1.2:+65979246	None:intergenic
CCTACTAGAATGTTGGGATT+AGG	0.337619	1.2:+65977390	None:intergenic
TTCTTTGAAGGCCAAAGATC+AGG	0.342290	1.2:-65979257	MS.gene20757:CDS
TTCAAAGAAGAGAATGGATT+TGG	0.342834	1.2:+65979271	None:intergenic
AAATCCATTCTCTTCTTTGA+AGG	0.351496	1.2:-65979269	MS.gene20757:CDS
CACATCCACTAGCTTCTTAC+TGG	0.357156	1.2:-65977771	MS.gene20757:CDS
TGCTCTTTAATTCCTGGAGC+TGG	0.363000	1.2:-65977955	MS.gene20757:CDS
TCATGGAGTGTTATTGAGTT+TGG	0.368140	1.2:-65978995	MS.gene20757:CDS
CAAGACATTCTCTGGTTAGA+AGG	0.391243	1.2:+65979227	None:intergenic
TCCAAACACCATTTATGTTC+AGG	0.394784	1.2:-65978891	MS.gene20757:intron
GGAGAAGGAAGAGGAAGGAA+AGG	0.397205	1.2:+65979393	None:intergenic
TTGTACCTGTTTCTTGGCTA+AGG	0.398642	1.2:+65977667	None:intergenic
GAAGGAAAGGGAAAGGGAAA+GGG	0.402545	1.2:+65979406	None:intergenic
GTAGGTTGGAGATGCTAAAA+AGG	0.402930	1.2:-65978781	MS.gene20757:intron
GCAGAGTTTGACAACACATT+TGG	0.404186	1.2:-65978203	MS.gene20757:CDS
ATGTACAATGTAGATTGATT+TGG	0.410845	1.2:-65979089	MS.gene20757:intron
GAAGAGGAAGGAAAGGGAAA+GGG	0.412680	1.2:+65979400	None:intergenic
TTGTTAGGATGAGTTGTTGT+GGG	0.416277	1.2:-65978306	MS.gene20757:intron
ACCTGAACATAAATGGTGTT+TGG	0.417646	1.2:+65978890	None:intergenic
CTAGTAGGTGCTGTTGTTGG+AGG	0.421002	1.2:-65977375	MS.gene20757:CDS
GGAAGGAAAGGGAAAGGGAA+AGG	0.423510	1.2:+65979405	None:intergenic
GCTTATCTTGCACACTCATT+CGG	0.425169	1.2:-65977258	MS.gene20757:CDS
GAGGACCAGATCAGCATGAT+AGG	0.431517	1.2:-65977356	MS.gene20757:CDS
GCTGGTGACAATGTCAAGTT+TGG	0.431994	1.2:-65979049	MS.gene20757:CDS
GGCGATGGAGTGGGAAGGCA+TGG	0.435448	1.2:+65979336	None:intergenic
CCTGGGTGTACAGCCATTGA+AGG	0.436838	1.2:+65977459	None:intergenic
TTGGCCTTCAAAGAAGAGAA+TGG	0.439709	1.2:+65979265	None:intergenic
GGAAGAGGAAGGAAAGGGAA+AGG	0.441118	1.2:+65979399	None:intergenic
CCCCTGTGGTGTATCCTTTG+TGG	0.441573	1.2:+65977492	None:intergenic
ATTCTAGTAGGTGCTGTTGT+TGG	0.445902	1.2:-65977378	MS.gene20757:CDS
CAAGTTAATGGTCAAATCCT+AGG	0.446620	1.2:-65978230	MS.gene20757:CDS
TGTTAGGTGGATTACTTGCT+AGG	0.447232	1.2:-65977564	MS.gene20757:intron
GTTTGACAACACATTTGGTT+GGG	0.449029	1.2:-65978198	MS.gene20757:CDS
GTTGGAGGGTATTATGATGC+TGG	0.454607	1.2:-65979067	MS.gene20757:CDS
CTGAACATTGGATCTTTCCT+GGG	0.455368	1.2:+65977442	None:intergenic
CAAAGTGAGGGTGGTGGCGA+TGG	0.456403	1.2:+65979321	None:intergenic
ACGCACAACAGAATGTGATT+TGG	0.456608	1.2:+65977727	None:intergenic
GGAAACCTATCATGCTGATC+TGG	0.457186	1.2:+65977351	None:intergenic
GAGAAAGAAGGAGAAGGAAG+AGG	0.457200	1.2:+65979384	None:intergenic
TATGTTAATGCACCACTTGT+GGG	0.460697	1.2:-65977288	MS.gene20757:CDS
TTTAGGAATTTCTAGTTCAA+AGG	0.464717	1.2:-65978023	MS.gene20757:intron
TTAGGAATTTCTAGTTCAAA+GGG	0.464908	1.2:-65978022	MS.gene20757:intron
CAGAACCTTAGCCAAGAAAC+AGG	0.467748	1.2:-65977672	MS.gene20757:intron
AGTTGTTGTGGGGTGCTGCT+TGG	0.467899	1.2:-65978295	MS.gene20757:CDS
ATGATAGGTTTCCAGATAAA+CGG	0.470251	1.2:-65977341	MS.gene20757:CDS
ATGTCATACATGGTGGGGTA+TGG	0.473593	1.2:-65977525	MS.gene20757:CDS
GGGATCATCACTTCCTTCAA+TGG	0.474143	1.2:-65977472	MS.gene20757:CDS
GCAGAAGAGGATCCAGCTCC+AGG	0.475179	1.2:+65977943	None:intergenic
ACCATGTATGACATCTTCAA+TGG	0.476433	1.2:+65977534	None:intergenic
CGAATAGCTTCTCTAGCATT+AGG	0.477541	1.2:+65978950	None:intergenic
GGAAAGATCCAATGTTCAGC+AGG	0.479066	1.2:-65977438	MS.gene20757:CDS
GGTGCATTAACATAAGTTGC+TGG	0.482529	1.2:+65977297	None:intergenic
ATGTAGATTGATTTGGTTGG+AGG	0.482748	1.2:-65979082	MS.gene20757:intron
TGAGGGTGGTGGCGATGGAG+TGG	0.482898	1.2:+65979326	None:intergenic
ACAGCACCTACTAGAATGTT+GGG	0.484006	1.2:+65977384	None:intergenic
AGCAAACGAAATTGTCTGAA+TGG	0.484341	1.2:+65977974	None:intergenic
AAGAAGGAGAAGGAAGAGGA+AGG	0.486021	1.2:+65979388	None:intergenic
TGAATGTAATTTAGGTACAT+TGG	0.486981	1.2:+65978251	None:intergenic
CCTAATCCCAACATTCTAGT+AGG	0.487578	1.2:-65977390	MS.gene20757:CDS
GAGAAGGAAGAGGAAGGAAA+GGG	0.487609	1.2:+65979394	None:intergenic
TGCTAGAGAAGCTATTCGTT+GGG	0.488469	1.2:-65978945	MS.gene20757:CDS
CACTGAACCCTGCTGAACAT+TGG	0.488575	1.2:+65977430	None:intergenic
AACAGCACCTACTAGAATGT+TGG	0.493607	1.2:+65977383	None:intergenic
TGGAGTGTTATTGAGTTTGG+TGG	0.494692	1.2:-65978992	MS.gene20757:CDS
GGAGTGGGAAGGCATGGGTA+TGG	0.495769	1.2:+65979342	None:intergenic
AGTTTGACAACACATTTGGT+TGG	0.497629	1.2:-65978199	MS.gene20757:CDS
TGTCAAACTCTGCAGCTCCT+AGG	0.498646	1.2:+65978213	None:intergenic
GAGGGTGGTGGCGATGGAGT+GGG	0.499968	1.2:+65979327	None:intergenic
TTATGTTAATGCACCACTTG+TGG	0.503544	1.2:-65977289	MS.gene20757:CDS
AAGATTGATGCAAACGCACC+TGG	0.504835	1.2:-65978705	MS.gene20757:CDS
AAAATCCCTCCATGACTACA+AGG	0.507819	1.2:-65977998	MS.gene20757:CDS
ATGCTAGAGAAGCTATTCGT+TGG	0.512170	1.2:-65978946	MS.gene20757:CDS
GGTTTCAAGACATTGCTGTA+AGG	0.515521	1.2:+65978456	None:intergenic
AGCAAGGATACTTCTTTCAA+AGG	0.517132	1.2:-65978159	MS.gene20757:intron
TACTAGAATGTTGGGATTAG+GGG	0.517279	1.2:+65977392	None:intergenic
CTGAATGGCCCTTGTAGTCA+TGG	0.518588	1.2:+65977989	None:intergenic
CTTCTAACCAGAGAATGTCT+TGG	0.519924	1.2:-65979226	MS.gene20757:CDS
AGAAGAGAGAAAGAAGGAGA+AGG	0.523733	1.2:+65979378	None:intergenic
CCAGAGTAATCACAGTAAAA+GGG	0.524276	1.2:+65978423	None:intergenic
CCCATTGAAGATGTCATACA+TGG	0.526434	1.2:-65977535	MS.gene20757:CDS
GGTTGGGATAACAAACATGT+TGG	0.527280	1.2:-65978182	MS.gene20757:CDS
TGTATCCTTTGTGGATACCT+TGG	0.528138	1.2:+65977501	None:intergenic
AAGAAGAGAAGAGAGAAAGA+AGG	0.528528	1.2:+65979372	None:intergenic
AGCAGCTGCTAATGCTGCAG+CGG	0.530570	1.2:+65978662	None:intergenic
TTTAGTGAGAAGGGCTATCA+GGG	0.533210	1.2:-65978598	MS.gene20757:intron
CCATGTATGACATCTTCAAT+GGG	0.535756	1.2:+65977535	None:intergenic
CTGGTCTCTCCCAACAAGCA+TGG	0.538685	1.2:+65978757	None:intergenic
TCTTTGAAGGCCAAAGATCA+GGG	0.542600	1.2:-65979256	MS.gene20757:CDS
TGTAGATTGATTTGGTTGGA+GGG	0.545437	1.2:-65979081	MS.gene20757:intron
CAGTAAAAGGGACAGACAAA+GGG	0.548513	1.2:+65978435	None:intergenic
GGAGTGTTATTGAGTTTGGT+GGG	0.550172	1.2:-65978991	MS.gene20757:CDS
GAGTTTGGTGGGTTGATGAA+AGG	0.552870	1.2:-65978980	MS.gene20757:CDS
ACAGTAAAAGGGACAGACAA+AGG	0.562752	1.2:+65978434	None:intergenic
CTTGTTAGGATGAGTTGTTG+TGG	0.568257	1.2:-65978307	MS.gene20757:intron
ACAATGTAGATTGATTTGGT+TGG	0.568750	1.2:-65979085	MS.gene20757:intron
AAAGACTCTGGTCTCTCTGA+TGG	0.569077	1.2:-65979200	MS.gene20757:CDS
GCGATGGAGTGGGAAGGCAT+GGG	0.569614	1.2:+65979337	None:intergenic
ATGTCTTGGAGAAAAGACTC+TGG	0.573843	1.2:-65979212	MS.gene20757:CDS
ATCCACAAAGGATACACCAC+AGG	0.581594	1.2:-65977494	MS.gene20757:CDS
TTGAAGATGTCATACATGGT+GGG	0.582564	1.2:-65977531	MS.gene20757:CDS
TAAGCAAGTGTTCCCACAAG+TGG	0.582903	1.2:+65977276	None:intergenic
TGGGGTATATTGTGCAGAAG+AGG	0.588731	1.2:+65977930	None:intergenic
TCTGCAGCAACTTCAGAACC+AGG	0.590338	1.2:+65978687	None:intergenic
TTGGGAGAGACCAGAAGACA+TGG	0.597392	1.2:-65978748	MS.gene20757:CDS
TCACATTCTGTTGTGCGTTG+TGG	0.597476	1.2:-65977722	MS.gene20757:CDS
CATTCTGTTGTGCGTTGTGG+TGG	0.598006	1.2:-65977719	MS.gene20757:CDS
TGTGAGTGGCAAAGTGAGGG+TGG	0.602326	1.2:+65979312	None:intergenic
TGAAGATGTCATACATGGTG+GGG	0.604622	1.2:-65977530	MS.gene20757:CDS
AACACTCCATGAAAGCATAG+TGG	0.607639	1.2:+65979006	None:intergenic
ATAACAAACATGTTGGAGCA+AGG	0.610200	1.2:-65978175	MS.gene20757:CDS
CCTTCAATGGCTGTACACCC+AGG	0.614449	1.2:-65977459	MS.gene20757:CDS
AGCTACCAGTAAGAAGCTAG+TGG	0.617412	1.2:+65977766	None:intergenic
TCTTCTGCACAATATACCCC+AGG	0.617991	1.2:-65977928	MS.gene20757:intron
GTGGTGGCGATGGAGTGGGA+AGG	0.619272	1.2:+65979331	None:intergenic
ATAGTGGTAGTAAAAGCCAT+AGG	0.628059	1.2:+65979022	None:intergenic
ATGGCCCTTGTAGTCATGGA+GGG	0.631433	1.2:+65977993	None:intergenic
TGTTAGGATGAGTTGTTGTG+GGG	0.636308	1.2:-65978305	MS.gene20757:intron
ATTGAAGATGTCATACATGG+TGG	0.647567	1.2:-65977532	MS.gene20757:CDS
ACATGGTGGGGTATGGACCA+AGG	0.656858	1.2:-65977518	MS.gene20757:CDS
TAGTTGTGAGTGGCAAAGTG+AGG	0.663280	1.2:+65979308	None:intergenic
AATGGCCCTTGTAGTCATGG+AGG	0.663344	1.2:+65977992	None:intergenic
GAAAGATCCAATGTTCAGCA+GGG	0.663959	1.2:-65977437	MS.gene20757:CDS
AGTTGTGAGTGGCAAAGTGA+GGG	0.678935	1.2:+65979309	None:intergenic
GAGTGGCAAAGTGAGGGTGG+TGG	0.679747	1.2:+65979315	None:intergenic
AGCATCTCTGTAGTTGTGAG+TGG	0.685689	1.2:+65979298	None:intergenic
ATGGACCAAGGTATCCACAA+AGG	0.706125	1.2:-65977506	MS.gene20757:CDS
TCCACAAAGGATACACCACA+GGG	0.706214	1.2:-65977493	MS.gene20757:CDS
CAGTTTGCTGATCAACACAG+AGG	0.707543	1.2:-65978480	MS.gene20757:CDS
AAATCCCTCCATGACTACAA+GGG	0.708720	1.2:-65977997	MS.gene20757:CDS
AAGGAAGTGATGATCCCCTG+TGG	0.807933	1.2:+65977478	None:intergenic
CCACAAAGGATACACCACAG+GGG	0.846463	1.2:-65977492	MS.gene20757:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATTTCATTATTTCAATT+AGG	+	chr1.2:65979022-65979041	None:intergenic	10.0%
!!!	TGATTTTGATTAATTTTTGT+TGG	-	chr1.2:65979064-65979083	MS.gene20757:CDS	15.0%
!!	GATTTATGCAACAAAAAAAA+AGG	+	chr1.2:65978124-65978143	None:intergenic	20.0%
!!!	ATATCTTTGTTGCTTATTTT+TGG	-	chr1.2:65977541-65977560	MS.gene20757:CDS	20.0%
!!!	ATTTTGTTAAGTAGAAAACA+TGG	-	chr1.2:65977823-65977842	MS.gene20757:intron	20.0%
!!!	CATTAACTTGAATGTAATTT+AGG	+	chr1.2:65978418-65978437	None:intergenic	20.0%
!!!	CTAAATTACATTCAAGTTAA+TGG	-	chr1.2:65978416-65978435	MS.gene20757:CDS	20.0%
!!!	TTTTGTTGGTATTTTTTGTT+AGG	-	chr1.2:65979078-65979097	MS.gene20757:CDS	20.0%
!	AAAACTAACCTGAACATAAA+TGG	+	chr1.2:65977778-65977797	None:intergenic	25.0%
!	AATGTTTGTTTGATACTATG+TGG	+	chr1.2:65978537-65978556	None:intergenic	25.0%
!	ATGTACAATGTAGATTGATT+TGG	-	chr1.2:65977569-65977588	MS.gene20757:intron	25.0%
!	TGAATGTAATTTAGGTACAT+TGG	+	chr1.2:65978410-65978429	None:intergenic	25.0%
!!!	AACATTGTTTTGTTTGTTTG+TGG	-	chr1.2:65978603-65978622	MS.gene20757:CDS	25.0%
!!!	ACTAAAATTTTGGAGTATGT+TGG	+	chr1.2:65978046-65978065	None:intergenic	25.0%
!!!	ATTATAGTACTTTTGACTGA+TGG	-	chr1.2:65978317-65978336	MS.gene20757:intron	25.0%
!!!	GGTAAATGAGTTTGATTTTT+GGG	-	chr1.2:65977844-65977863	MS.gene20757:intron	25.0%
!!!	TATCTCAGTTTTAACTCTAT+TGG	-	chr1.2:65978771-65978790	MS.gene20757:CDS	25.0%
!!!	TGGTAAATGAGTTTGATTTT+TGG	-	chr1.2:65977843-65977862	MS.gene20757:intron	25.0%
!!!	TTAGGAATTTCTAGTTCAAA+GGG	-	chr1.2:65978636-65978655	MS.gene20757:CDS	25.0%
!!!	TTATAGTACTTTTGACTGAT+GGG	-	chr1.2:65978318-65978337	MS.gene20757:intron	25.0%
!!!	TTTAGGAATTTCTAGTTCAA+AGG	-	chr1.2:65978635-65978654	MS.gene20757:CDS	25.0%
	AAAACTGAGATAGAAGAAAG+AGG	+	chr1.2:65978763-65978782	None:intergenic	30.0%
	AAACTGAGATAGAAGAAAGA+GGG	+	chr1.2:65978762-65978781	None:intergenic	30.0%
	AAATCCATTCTCTTCTTTGA+AGG	-	chr1.2:65977389-65977408	MS.gene20757:CDS	30.0%
	AGTATGACTCAAGTAAAAAC+TGG	+	chr1.2:65978293-65978312	None:intergenic	30.0%
	ATGATAGGTTTCCAGATAAA+CGG	-	chr1.2:65979317-65979336	MS.gene20757:CDS	30.0%
	TCAATCTTGAAAACACTTCT+TGG	+	chr1.2:65977941-65977960	None:intergenic	30.0%
	TTTGCAGAAATGTTTGTTTG+TGG	+	chr1.2:65978563-65978582	None:intergenic	30.0%
!	AGTTTTTCTTCTTCTGTTCA+TGG	-	chr1.2:65977792-65977811	MS.gene20757:CDS	30.0%
!	TTCAAAGAAGAGAATGGATT+TGG	+	chr1.2:65977390-65977409	None:intergenic	30.0%
!!	ACAATGTAGATTGATTTGGT+TGG	-	chr1.2:65977573-65977592	MS.gene20757:intron	30.0%
!!	ACTCCAAAATTTTAGTGAGA+AGG	-	chr1.2:65978050-65978069	MS.gene20757:intron	30.0%
!!	ATGTGATTTGGTCAAGTATT+TGG	+	chr1.2:65978922-65978941	None:intergenic	30.0%
!!	CTCCAAAATTTTAGTGAGAA+GGG	-	chr1.2:65978051-65978070	MS.gene20757:intron	30.0%
!!	CTGAGATTGTGTTTGTTTTT+GGG	-	chr1.2:65978143-65978162	MS.gene20757:intron	30.0%
!!	GTATTTTTGTACCTGTTTCT+TGG	+	chr1.2:65979000-65979019	None:intergenic	30.0%
!!	TCTGAGATTGTGTTTGTTTT+TGG	-	chr1.2:65978142-65978161	MS.gene20757:intron	30.0%
!!	TTAACTCTATTGGTTTCATG+TGG	-	chr1.2:65978781-65978800	MS.gene20757:intron	30.0%
!!!	GTTTGTGGTTTTGTGATTTT+AGG	-	chr1.2:65978618-65978637	MS.gene20757:CDS	30.0%
!!!	TGAGATTGTGTTTGTTTTTG+GGG	-	chr1.2:65978144-65978163	MS.gene20757:intron	30.0%
!!!	TGTTGGTATTTTTTGTTAGG+TGG	-	chr1.2:65979081-65979100	MS.gene20757:intron	30.0%
!!!	TTTTTGGGTTTTTTGTTCGT+AGG	-	chr1.2:65977859-65977878	MS.gene20757:intron	30.0%
	AAGAAGAGAAGAGAGAAAGA+AGG	+	chr1.2:65977289-65977308	None:intergenic	35.0%
	ACCAGAGTAATCACAGTAAA+AGG	+	chr1.2:65978239-65978258	None:intergenic	35.0%
	ACCATGTATGACATCTTCAA+TGG	+	chr1.2:65979127-65979146	None:intergenic	35.0%
	ACCTGAACATAAATGGTGTT+TGG	+	chr1.2:65977771-65977790	None:intergenic	35.0%
	AGCAAACGAAATTGTCTGAA+TGG	+	chr1.2:65978687-65978706	None:intergenic	35.0%
	AGCAAGGATACTTCTTTCAA+AGG	-	chr1.2:65978499-65978518	MS.gene20757:intron	35.0%
	AGTTTGACAACACATTTGGT+TGG	-	chr1.2:65978459-65978478	MS.gene20757:CDS	35.0%
	ATAACAAACATGTTGGAGCA+AGG	-	chr1.2:65978483-65978502	MS.gene20757:CDS	35.0%
	ATAGTGGTAGTAAAAGCCAT+AGG	+	chr1.2:65977639-65977658	None:intergenic	35.0%
	ATTGAAGATGTCATACATGG+TGG	-	chr1.2:65979126-65979145	MS.gene20757:intron	35.0%
	CAAGTTAATGGTCAAATCCT+AGG	-	chr1.2:65978428-65978447	MS.gene20757:CDS	35.0%
	CCAGAGTAATCACAGTAAAA+GGG	+	chr1.2:65978238-65978257	None:intergenic	35.0%
	CCATGTATGACATCTTCAAT+GGG	+	chr1.2:65979126-65979145	None:intergenic	35.0%
	CTACTAGAATGTTGGGATTA+GGG	+	chr1.2:65979270-65979289	None:intergenic	35.0%
	GATACTATGTGGTGACATTT+AGG	+	chr1.2:65978526-65978545	None:intergenic	35.0%
	GCTCAAAAACAGTTACAACT+TGG	+	chr1.2:65978808-65978827	None:intergenic	35.0%
	GTTTGACAACACATTTGGTT+GGG	-	chr1.2:65978460-65978479	MS.gene20757:CDS	35.0%
	TAAAAAGGACCATGCTTGTT+GGG	-	chr1.2:65977892-65977911	MS.gene20757:intron	35.0%
	TACTAGAATGTTGGGATTAG+GGG	+	chr1.2:65979269-65979288	None:intergenic	35.0%
	TATGTTAATGCACCACTTGT+GGG	-	chr1.2:65979370-65979389	MS.gene20757:CDS	35.0%
	TCAAGTAAAAACTGGCACAA+TGG	+	chr1.2:65978285-65978304	None:intergenic	35.0%
	TCATAATCTGACCGTTTATC+TGG	+	chr1.2:65979331-65979350	None:intergenic	35.0%
	TCCAAACACCATTTATGTTC+AGG	-	chr1.2:65977767-65977786	MS.gene20757:CDS	35.0%
	TTACTACCACTATGCTTTCA+TGG	-	chr1.2:65977646-65977665	MS.gene20757:intron	35.0%
	TTATGTTAATGCACCACTTG+TGG	-	chr1.2:65979369-65979388	MS.gene20757:CDS	35.0%
	TTCGTTTGCTCTTTAATTCC+TGG	-	chr1.2:65978697-65978716	MS.gene20757:CDS	35.0%
	TTGAAGATGTCATACATGGT+GGG	-	chr1.2:65979127-65979146	MS.gene20757:intron	35.0%
	TTGAGCTTGTGAAACTTTAC+AGG	-	chr1.2:65978822-65978841	MS.gene20757:intron	35.0%
	TTGTTAGGATGAGTTGTTGT+GGG	-	chr1.2:65978352-65978371	MS.gene20757:intron	35.0%
!	AGCCCTTCTCACTAAAATTT+TGG	+	chr1.2:65978056-65978075	None:intergenic	35.0%
!	TCATGGAGTGTTATTGAGTT+TGG	-	chr1.2:65977663-65977682	MS.gene20757:intron	35.0%
!!	ATGTAGATTGATTTGGTTGG+AGG	-	chr1.2:65977576-65977595	MS.gene20757:intron	35.0%
!!	TGGGTTTGTAATGACTTGTT+AGG	-	chr1.2:65978337-65978356	MS.gene20757:intron	35.0%
!!	TGTAGATTGATTTGGTTGGA+GGG	-	chr1.2:65977577-65977596	MS.gene20757:intron	35.0%
!!!	TGTCAAGTTTGGTTTTCCTA+TGG	-	chr1.2:65977620-65977639	MS.gene20757:intron	35.0%
!!!	TGTGTTTGTTTTTGGGGTTT+AGG	-	chr1.2:65978150-65978169	MS.gene20757:intron	35.0%
	AAAATCCCTCCATGACTACA+AGG	-	chr1.2:65978660-65978679	MS.gene20757:CDS	40.0%
	AAATCCCTCCATGACTACAA+GGG	-	chr1.2:65978661-65978680	MS.gene20757:CDS	40.0%
	AACAGCACCTACTAGAATGT+TGG	+	chr1.2:65979278-65979297	None:intergenic	40.0%
	ACAGCACCTACTAGAATGTT+GGG	+	chr1.2:65979277-65979296	None:intergenic	40.0%
	ACAGTAAAAGGGACAGACAA+AGG	+	chr1.2:65978227-65978246	None:intergenic	40.0%
	ACGCACAACAGAATGTGATT+TGG	+	chr1.2:65978934-65978953	None:intergenic	40.0%
	AGAAGAGAGAAAGAAGGAGA+AGG	+	chr1.2:65977283-65977302	None:intergenic	40.0%
	AGCTTGTGAAACTTTACAGG+TGG	-	chr1.2:65978825-65978844	MS.gene20757:intron	40.0%
	ATGCTAGAGAAGCTATTCGT+TGG	-	chr1.2:65977712-65977731	MS.gene20757:CDS	40.0%
	ATGTCTTGGAGAAAAGACTC+TGG	-	chr1.2:65977446-65977465	MS.gene20757:CDS	40.0%
	CAAGACATTCTCTGGTTAGA+AGG	+	chr1.2:65977434-65977453	None:intergenic	40.0%
	CAGTAAAAGGGACAGACAAA+GGG	+	chr1.2:65978226-65978245	None:intergenic	40.0%
	CCCATTGAAGATGTCATACA+TGG	-	chr1.2:65979123-65979142	MS.gene20757:intron	40.0%
	CCTAATCCCAACATTCTAGT+AGG	-	chr1.2:65979268-65979287	MS.gene20757:CDS	40.0%
	CCTACTAGAATGTTGGGATT+AGG	+	chr1.2:65979271-65979290	None:intergenic	40.0%
	CGAATAGCTTCTCTAGCATT+AGG	+	chr1.2:65977711-65977730	None:intergenic	40.0%
	CTAAAAAGGACCATGCTTGT+TGG	-	chr1.2:65977891-65977910	MS.gene20757:intron	40.0%
	CTGAACATTGGATCTTTCCT+GGG	+	chr1.2:65979219-65979238	None:intergenic	40.0%
	CTGTGATTACTCTGGTTATC+AGG	-	chr1.2:65978243-65978262	MS.gene20757:CDS	40.0%
	CTTCTAACCAGAGAATGTCT+TGG	-	chr1.2:65977432-65977451	MS.gene20757:CDS	40.0%
	CTTGTTAGGATGAGTTGTTG+TGG	-	chr1.2:65978351-65978370	MS.gene20757:intron	40.0%
	GAAAGATCCAATGTTCAGCA+GGG	-	chr1.2:65979221-65979240	MS.gene20757:CDS	40.0%
	GCAGAGTTTGACAACACATT+TGG	-	chr1.2:65978455-65978474	MS.gene20757:CDS	40.0%
	GCTTATCTTGCACACTCATT+CGG	-	chr1.2:65979400-65979419	MS.gene20757:CDS	40.0%
	GCTTGTGAAACTTTACAGGT+GGG	-	chr1.2:65978826-65978845	MS.gene20757:intron	40.0%
	GGTGCATTAACATAAGTTGC+TGG	+	chr1.2:65979364-65979383	None:intergenic	40.0%
	GGTTGGGATAACAAACATGT+TGG	-	chr1.2:65978476-65978495	MS.gene20757:CDS	40.0%
	GGTTTCAAGACATTGCTGTA+AGG	+	chr1.2:65978205-65978224	None:intergenic	40.0%
	TGAAGATGTCATACATGGTG+GGG	-	chr1.2:65979128-65979147	MS.gene20757:intron	40.0%
	TGCTAGAGAAGCTATTCGTT+GGG	-	chr1.2:65977713-65977732	MS.gene20757:CDS	40.0%
	TGTATCCTTTGTGGATACCT+TGG	+	chr1.2:65979160-65979179	None:intergenic	40.0%
	TGTTAGGATGAGTTGTTGTG+GGG	-	chr1.2:65978353-65978372	MS.gene20757:intron	40.0%
	TTTAGTGAGAAGGGCTATCA+GGG	-	chr1.2:65978060-65978079	MS.gene20757:intron	40.0%
!	AACACTCCATGAAAGCATAG+TGG	+	chr1.2:65977655-65977674	None:intergenic	40.0%
!	CCCTTTTACTGTGATTACTC+TGG	-	chr1.2:65978235-65978254	MS.gene20757:CDS	40.0%
!	CTTTTCTCCAAGACATTCTC+TGG	+	chr1.2:65977442-65977461	None:intergenic	40.0%
!	GGAGTGTTATTGAGTTTGGT+GGG	-	chr1.2:65977667-65977686	MS.gene20757:intron	40.0%
!	GTAGGTTGGAGATGCTAAAA+AGG	-	chr1.2:65977877-65977896	MS.gene20757:intron	40.0%
!	TCTTTGAAGGCCAAAGATCA+GGG	-	chr1.2:65977402-65977421	MS.gene20757:CDS	40.0%
!	TGGAGTGTTATTGAGTTTGG+TGG	-	chr1.2:65977666-65977685	MS.gene20757:intron	40.0%
!	TTCTTTGAAGGCCAAAGATC+AGG	-	chr1.2:65977401-65977420	MS.gene20757:CDS	40.0%
!	TTGGCCTTCAAAGAAGAGAA+TGG	+	chr1.2:65977396-65977415	None:intergenic	40.0%
!	TTGTACCTGTTTCTTGGCTA+AGG	+	chr1.2:65978994-65979013	None:intergenic	40.0%
!	TTTTAGTGAGAAGGGCTATC+AGG	-	chr1.2:65978059-65978078	MS.gene20757:intron	40.0%
!!	ATTCTAGTAGGTGCTGTTGT+TGG	-	chr1.2:65979280-65979299	MS.gene20757:CDS	40.0%
!!	GAGTCTCTTTGGTGTTACTA+TGG	+	chr1.2:65978967-65978986	None:intergenic	40.0%
!!	TGTTAGGTGGATTACTTGCT+AGG	-	chr1.2:65979094-65979113	MS.gene20757:intron	40.0%
!!!	TGGGTTTTTTGTTCGTAGGT+TGG	-	chr1.2:65977863-65977882	MS.gene20757:intron	40.0%
	AAAGACTCTGGTCTCTCTGA+TGG	-	chr1.2:65977458-65977477	MS.gene20757:CDS	45.0%
	AAGAAAGAGGGACCTTACCT+GGG	+	chr1.2:65978750-65978769	None:intergenic	45.0%
	AAGAAGGAGAAGGAAGAGGA+AGG	+	chr1.2:65977273-65977292	None:intergenic	45.0%
	AAGATTGATGCAAACGCACC+TGG	-	chr1.2:65977953-65977972	MS.gene20757:CDS	45.0%
	AAGGTAGCTTCCCTGATCTT+TGG	+	chr1.2:65977415-65977434	None:intergenic	45.0%
	AGCATCTCTGTAGTTGTGAG+TGG	+	chr1.2:65977363-65977382	None:intergenic	45.0%
	AGCTACCAGTAAGAAGCTAG+TGG	+	chr1.2:65978895-65978914	None:intergenic	45.0%
	AGTTGTGAGTGGCAAAGTGA+GGG	+	chr1.2:65977352-65977371	None:intergenic	45.0%
	ATCCACAAAGGATACACCAC+AGG	-	chr1.2:65979164-65979183	MS.gene20757:intron	45.0%
	CAGAACCTTAGCCAAGAAAC+AGG	-	chr1.2:65978986-65979005	MS.gene20757:CDS	45.0%
	GAAGAGGAAGGAAAGGGAAA+GGG	+	chr1.2:65977261-65977280	None:intergenic	45.0%
	GAAGGAAAGGGAAAGGGAAA+GGG	+	chr1.2:65977255-65977274	None:intergenic	45.0%
	GAGAAAGAAGGAGAAGGAAG+AGG	+	chr1.2:65977277-65977296	None:intergenic	45.0%
	GAGAAGGAAGAGGAAGGAAA+GGG	+	chr1.2:65977267-65977286	None:intergenic	45.0%
	GCTGAACATTGGATCTTTCC+TGG	+	chr1.2:65979220-65979239	None:intergenic	45.0%
	GCTGGTGACAATGTCAAGTT+TGG	-	chr1.2:65977609-65977628	MS.gene20757:intron	45.0%
	GGAAACCTATCATGCTGATC+TGG	+	chr1.2:65979310-65979329	None:intergenic	45.0%
	GGAAAGATCCAATGTTCAGC+AGG	-	chr1.2:65979220-65979239	MS.gene20757:CDS	45.0%
	GGGATCATCACTTCCTTCAA+TGG	-	chr1.2:65979186-65979205	MS.gene20757:intron	45.0%
	TAAGCAAGTGTTCCCACAAG+TGG	+	chr1.2:65979385-65979404	None:intergenic	45.0%
	TAGTTGTGAGTGGCAAAGTG+AGG	+	chr1.2:65977353-65977372	None:intergenic	45.0%
	TCACATTCTGTTGTGCGTTG+TGG	-	chr1.2:65978936-65978955	MS.gene20757:CDS	45.0%
	TCCACAAAGGATACACCACA+GGG	-	chr1.2:65979165-65979184	MS.gene20757:intron	45.0%
	TCTTCTGCACAATATACCCC+AGG	-	chr1.2:65978730-65978749	MS.gene20757:CDS	45.0%
	TGCACAATATACCCCAGGTA+AGG	-	chr1.2:65978735-65978754	MS.gene20757:CDS	45.0%
	TGCTCTTTAATTCCTGGAGC+TGG	-	chr1.2:65978703-65978722	MS.gene20757:CDS	45.0%
	TGGGGTATATTGTGCAGAAG+AGG	+	chr1.2:65978731-65978750	None:intergenic	45.0%
!	ATGGACCAAGGTATCCACAA+AGG	-	chr1.2:65979152-65979171	MS.gene20757:intron	45.0%
!	ATGTCATACATGGTGGGGTA+TGG	-	chr1.2:65979133-65979152	MS.gene20757:intron	45.0%
!	CACATCCACTAGCTTCTTAC+TGG	-	chr1.2:65978887-65978906	MS.gene20757:intron	45.0%
!!	CAGTTTGCTGATCAACACAG+AGG	-	chr1.2:65978178-65978197	MS.gene20757:CDS	45.0%
!!	GAGTTTGGTGGGTTGATGAA+AGG	-	chr1.2:65977678-65977697	MS.gene20757:CDS	45.0%
!!	GCTAAGGTTCTGAGTCTCTT+TGG	+	chr1.2:65978978-65978997	None:intergenic	45.0%
!!	GTTGGAGGGTATTATGATGC+TGG	-	chr1.2:65977591-65977610	MS.gene20757:intron	45.0%
	AAGGAAGTGATGATCCCCTG+TGG	+	chr1.2:65979183-65979202	None:intergenic	50.0%
	AATGGCCCTTGTAGTCATGG+AGG	+	chr1.2:65978669-65978688	None:intergenic	50.0%
	AGAAAGAGGGACCTTACCTG+GGG	+	chr1.2:65978749-65978768	None:intergenic	50.0%
	ATGGCCCTTGTAGTCATGGA+GGG	+	chr1.2:65978668-65978687	None:intergenic	50.0%
	CACTGAACCCTGCTGAACAT+TGG	+	chr1.2:65979231-65979250	None:intergenic	50.0%
	CATTCTGTTGTGCGTTGTGG+TGG	-	chr1.2:65978939-65978958	MS.gene20757:CDS	50.0%
	CCACAAAGGATACACCACAG+GGG	-	chr1.2:65979166-65979185	MS.gene20757:intron	50.0%
	CTGAATGGCCCTTGTAGTCA+TGG	+	chr1.2:65978672-65978691	None:intergenic	50.0%
	GAAGAAAGAGGGACCTTACC+TGG	+	chr1.2:65978751-65978770	None:intergenic	50.0%
	GAGGACCAGATCAGCATGAT+AGG	-	chr1.2:65979302-65979321	MS.gene20757:CDS	50.0%
	GGAAGAGGAAGGAAAGGGAA+AGG	+	chr1.2:65977262-65977281	None:intergenic	50.0%
	GGAAGGAAAGGGAAAGGGAA+AGG	+	chr1.2:65977256-65977275	None:intergenic	50.0%
	GGAGAAGGAAGAGGAAGGAA+AGG	+	chr1.2:65977268-65977287	None:intergenic	50.0%
	TCTGCAGCAACTTCAGAACC+AGG	+	chr1.2:65977974-65977993	None:intergenic	50.0%
	TGTCAAACTCTGCAGCTCCT+AGG	+	chr1.2:65978448-65978467	None:intergenic	50.0%
	TTGGGAGAGACCAGAAGACA+TGG	-	chr1.2:65977910-65977929	MS.gene20757:intron	50.0%
!	CTTGGTGTGTCCATGTCTTC+TGG	+	chr1.2:65977923-65977942	None:intergenic	50.0%
!!	CTAGTAGGTGCTGTTGTTGG+AGG	-	chr1.2:65979283-65979302	MS.gene20757:CDS	50.0%
	AGCAGCTGCTAATGCTGCAG+CGG	+	chr1.2:65977999-65978018	None:intergenic	55.0%
	CCCCTGTGGTGTATCCTTTG+TGG	+	chr1.2:65979169-65979188	None:intergenic	55.0%
	CCTGGGTGTACAGCCATTGA+AGG	+	chr1.2:65979202-65979221	None:intergenic	55.0%
	CCTTCAATGGCTGTACACCC+AGG	-	chr1.2:65979199-65979218	MS.gene20757:CDS	55.0%
	CTGGTCTCTCCCAACAAGCA+TGG	+	chr1.2:65977904-65977923	None:intergenic	55.0%
	TGTGAGTGGCAAAGTGAGGG+TGG	+	chr1.2:65977349-65977368	None:intergenic	55.0%
!	ACATGGTGGGGTATGGACCA+AGG	-	chr1.2:65979140-65979159	MS.gene20757:intron	55.0%
!!	AGTTGTTGTGGGGTGCTGCT+TGG	-	chr1.2:65978363-65978382	MS.gene20757:intron	55.0%
	GAGTGGCAAAGTGAGGGTGG+TGG	+	chr1.2:65977346-65977365	None:intergenic	60.0%
	GCAGAAGAGGATCCAGCTCC+AGG	+	chr1.2:65978718-65978737	None:intergenic	60.0%
!	CAAAGTGAGGGTGGTGGCGA+TGG	+	chr1.2:65977340-65977359	None:intergenic	60.0%
!	GCGATGGAGTGGGAAGGCAT+GGG	+	chr1.2:65977324-65977343	None:intergenic	60.0%
!	GGAGTGGGAAGGCATGGGTA+TGG	+	chr1.2:65977319-65977338	None:intergenic	60.0%
	GGCGATGGAGTGGGAAGGCA+TGG	+	chr1.2:65977325-65977344	None:intergenic	65.0%
!	GAGGGTGGTGGCGATGGAGT+GGG	+	chr1.2:65977334-65977353	None:intergenic	65.0%
!	GTGGTGGCGATGGAGTGGGA+AGG	+	chr1.2:65977330-65977349	None:intergenic	65.0%
!	TGAGGGTGGTGGCGATGGAG+TGG	+	chr1.2:65977335-65977354	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	65977248	65979432	65977248	ID=MS.gene20757
chr1.2	mRNA	65977248	65979432	65977248	ID=MS.gene20757.t1;Parent=MS.gene20757
chr1.2	exon	65979187	65979432	65979187	ID=MS.gene20757.t1.exon1;Parent=MS.gene20757.t1
chr1.2	CDS	65979187	65979432	65979187	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65978892	65979098	65978892	ID=MS.gene20757.t1.exon2;Parent=MS.gene20757.t1
chr1.2	CDS	65978892	65979098	65978892	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65978599	65978799	65978599	ID=MS.gene20757.t1.exon3;Parent=MS.gene20757.t1
chr1.2	CDS	65978599	65978799	65978599	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65978416	65978508	65978416	ID=MS.gene20757.t1.exon4;Parent=MS.gene20757.t1
chr1.2	CDS	65978416	65978508	65978416	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65978160	65978321	65978160	ID=MS.gene20757.t1.exon5;Parent=MS.gene20757.t1
chr1.2	CDS	65978160	65978321	65978160	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65977929	65978040	65977929	ID=MS.gene20757.t1.exon6;Parent=MS.gene20757.t1
chr1.2	CDS	65977929	65978040	65977929	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65977673	65977836	65977673	ID=MS.gene20757.t1.exon7;Parent=MS.gene20757.t1
chr1.2	CDS	65977673	65977836	65977673	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1
chr1.2	exon	65977248	65977580	65977248	ID=MS.gene20757.t1.exon8;Parent=MS.gene20757.t1
chr1.2	CDS	65977248	65977580	65977248	ID=cds.MS.gene20757.t1;Parent=MS.gene20757.t1

Gene Sequence

>MS.gene20757

ATGACCCTTTCCCTTTCCCTTTCCTTCCTCTTCCTTCTCCTTCTTTCTCTCTTCTCTTCTTTCATTCTCCATACCCATGCCTTCCCACTCCATCGCCACCACCCTCACTTTGCCACTCACAACTACAGAGATGCTCTCACCAAATCCATTCTCTTCTTTGAAGGCCAAAGATCAGGGAAGCTACCTTCTAACCAGAGAATGTCTTGGAGAAAAGACTCTGGTCTCTCTGATGGCTCAGCAATGCATGTATGTTAACAAATCATATCATAAATCTCACATTATCATGTATTTCTATATCTTTGTTGCTTATTTTTGGCTTAAATGTACAATGTAGATTGATTTGGTTGGAGGGTATTATGATGCTGGTGACAATGTCAAGTTTGGTTTTCCTATGGCTTTTACTACCACTATGCTTTCATGGAGTGTTATTGAGTTTGGTGGGTTGATGAAAGGTGAGTTGCCTAATGCTAGAGAAGCTATTCGTTGGGCTACTGATTATCTTCTAAAAGCTACTGCACATCCAAACACCATTTATGTTCAGGTTAGTTTTTCTTCTTCTGTTCATGGTTTTTGAGATTTTGTTAAGTAGAAAACATGGTAAATGAGTTTGATTTTTGGGTTTTTTGTTCGTAGGTTGGAGATGCTAAAAAGGACCATGCTTGTTGGGAGAGACCAGAAGACATGGACACACCAAGAAGTGTTTTCAAGATTGATGCAAACGCACCTGGTTCTGAAGTTGCTGCAGAAACCGCTGCAGCATTAGCAGCTGCTTCTCTTGTTTTTAGAAGAACTGATCCAACATACTCCAAAATTTTAGTGAGAAGGGCTATCAGGGTAAGCTTGATTCATGATAAAAAAAATTAAAAAATGTTTCCTTTTTTTTTGTTGCATAAATCTGAGATTGTGTTTGTTTTTGGGGTTTAGGTTTTTCAGTTTGCTGATCAACACAGAGGACCTTACAGCAATGTCTTGAAACCCTTTGTCTGTCCCTTTTACTGTGATTACTCTGGTTATCAGGTAAAGTTTTGATTTTTCCATTGTGCCAGTTTTTACTTGAGTCATACTCATAATTATAGTACTTTTGACTGATGGGTTTGTAATGACTTGTTAGGATGAGTTGTTGTGGGGTGCTGCTTGGTTACACAAAGCTACAAAGAATCCAATGTACCTAAATTACATTCAAGTTAATGGTCAAATCCTAGGAGCTGCAGAGTTTGACAACACATTTGGTTGGGATAACAAACATGTTGGAGCAAGGATACTTCTTTCAAAGGTCCTAAATGTCACCACATAGTATCAAACAAACATTTCACCACAAACAAACATTTCTGCAAACGAAATAATGTTTTAAAACTAACATTGTTTTGTTTGTTTGTGGTTTTGTGATTTTAGGAATTTCTAGTTCAAAGGGTAAAATCCCTCCATGACTACAAGGGCCATTCAGACAATTTCGTTTGCTCTTTAATTCCTGGAGCTGGATCCTCTTCTGCACAATATACCCCAGGTAAGGTCCCTCTTTCTTCTATCTCAGTTTTAACTCTATTGGTTTCATGTGGTCCAAGTTGTAACTGTTTTTGAGCTTGTGAAACTTTACAGGTGGGCTTTTGTTCAAGATGAGTGATAGTAACATGCAGTATGTCACATCCACTAGCTTCTTACTGGTAGCTTATGCCAAATACTTGACCAAATCACATTCTGTTGTGCGTTGTGGTGGTACCATAGTAACACCAAAGAGACTCAGAACCTTAGCCAAGAAACAGGTACAAAAATACCTAATTGAAATAATGAAATTTATTTGATTCAAGTAGTATCATTTTGATTTTGATTAATTTTTGTTGGTATTTTTTGTTAGGTGGATTACTTGCTAGGAGATAACCCATTGAAGATGTCATACATGGTGGGGTATGGACCAAGGTATCCACAAAGGATACACCACAGGGGATCATCACTTCCTTCAATGGCTGTACACCCAGGAAAGATCCAATGTTCAGCAGGGTTCAGTGTGATGAGTTCACAATCCCCTAATCCCAACATTCTAGTAGGTGCTGTTGTTGGAGGACCAGATCAGCATGATAGGTTTCCAGATAAACGGTCAGATTATGAGCAATCAGAGCCAGCAACTTATGTTAATGCACCACTTGTGGGAACACTTGCTTATCTTGCACACTCATTCGGTCAACTTTAG

Protein sequence

>MS.gene20757.t1

MTLSLSLSFLFLLLLSLFSSFILHTHAFPLHRHHPHFATHNYRDALTKSILFFEGQRSGKLPSNQRMSWRKDSGLSDGSAMHIDLVGGYYDAGDNVKFGFPMAFTTTMLSWSVIEFGGLMKGELPNAREAIRWATDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKIDANAPGSEVAAETAAALAAASLVFRRTDPTYSKILVRRAIRVFQFADQHRGPYSNVLKPFVCPFYCDYSGYQDELLWGAAWLHKATKNPMYLNYIQVNGQILGAAEFDNTFGWDNKHVGARILLSKEFLVQRVKSLHDYKGHSDNFVCSLIPGAGSSSAQYTPGGLLFKMSDSNMQYVTSTSFLLVAYAKYLTKSHSVVRCGGTIVTPKRLRTLAKKQVDYLLGDNPLKMSYMVGYGPRYPQRIHHRGSSLPSMAVHPGKIQCSAGFSVMSSQSPNPNILVGAVVGGPDQHDRFPDKRSDYEQSEPATYVNAPLVGTLAYLAHSFGQL