AlfalfaGEDB | MS.gene34078

M. sativa cultivar XinJiangDaYe / MS.gene34078

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00514.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34078.t1	PNX61172.1	92.7	191	14	0	1	191	1	191	7.10E-68	266.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34078.t1	Q5VRH9	62.6	107	39	1	83	189	502	607	6.6e-30	132.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34078.t1	A0A2K3K4I0	92.7	191	14	0	1	191	1	191	5.1e-68	266.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene34078	0.822209	2.68E-53	-1.69E-46
MS.gene04920	MS.gene34078	0.814435	1.54E-51	-1.69E-46
MS.gene049283	MS.gene34078	0.883154	5.70E-71	-1.69E-46
MS.gene049499	MS.gene34078	0.829675	4.51E-55	-1.69E-46
MS.gene049680	MS.gene34078	0.811859	5.67E-51	-1.69E-46
MS.gene049983	MS.gene34078	0.847071	1.46E-59	-1.69E-46
MS.gene04998	MS.gene34078	0.800901	1.16E-48	-1.69E-46
MS.gene050571	MS.gene34078	0.802262	6.10E-49	-1.69E-46
MS.gene050646	MS.gene34078	0.818457	1.94E-52	-1.69E-46
MS.gene050654	MS.gene34078	0.80424	2.38E-49	-1.69E-46
MS.gene050945	MS.gene34078	0.833592	4.88E-56	-1.69E-46
MS.gene051234	MS.gene34078	0.804773	1.84E-49	-1.69E-46
MS.gene051343	MS.gene34078	0.8141	1.83E-51	-1.69E-46
MS.gene051597	MS.gene34078	0.813111	3.02E-51	-1.69E-46
MS.gene051676	MS.gene34078	0.817658	2.94E-52	-1.69E-46
MS.gene051699	MS.gene34078	0.8223	2.55E-53	-1.69E-46
MS.gene051902	MS.gene34078	0.833758	4.44E-56	-1.69E-46
MS.gene051975	MS.gene34078	0.838659	2.52E-57	-1.69E-46
MS.gene052016	MS.gene34078	0.810887	9.22E-51	-1.69E-46
MS.gene052118	MS.gene34078	0.812885	3.38E-51	-1.69E-46
MS.gene052463	MS.gene34078	0.806227	9.11E-50	-1.69E-46
MS.gene052533	MS.gene34078	0.834406	3.05E-56	-1.69E-46
MS.gene053298	MS.gene34078	0.840468	8.54E-58	-1.69E-46
MS.gene053879	MS.gene34078	0.801078	1.07E-48	-1.69E-46
MS.gene053931	MS.gene34078	0.808131	3.60E-50	-1.69E-46
MS.gene053963	MS.gene34078	0.844836	5.91E-59	-1.69E-46
MS.gene054222	MS.gene34078	0.834252	3.34E-56	-1.69E-46
MS.gene054413	MS.gene34078	0.809321	2.01E-50	-1.69E-46
MS.gene05458	MS.gene34078	0.810256	1.26E-50	-1.69E-46
MS.gene054892	MS.gene34078	0.859317	4.50E-63	-1.69E-46
MS.gene054893	MS.gene34078	0.856035	4.22E-62	-1.69E-46
MS.gene054957	MS.gene34078	0.865842	4.40E-65	-1.69E-46
MS.gene055229	MS.gene34078	0.802432	5.63E-49	-1.69E-46
MS.gene055513	MS.gene34078	0.819526	1.11E-52	-1.69E-46
MS.gene056315	MS.gene34078	0.803176	3.95E-49	-1.69E-46
MS.gene056403	MS.gene34078	0.815038	1.13E-51	-1.69E-46
MS.gene056699	MS.gene34078	0.837163	6.11E-57	-1.69E-46
MS.gene05686	MS.gene34078	0.804657	1.94E-49	-1.69E-46
MS.gene056973	MS.gene34078	0.807738	4.37E-50	-1.69E-46
MS.gene056986	MS.gene34078	0.830469	2.89E-55	-1.69E-46
MS.gene057102	MS.gene34078	0.867286	1.53E-65	-1.69E-46
MS.gene057155	MS.gene34078	0.825531	4.46E-54	-1.69E-46
MS.gene057208	MS.gene34078	0.826069	3.32E-54	-1.69E-46
MS.gene057622	MS.gene34078	0.822462	2.34E-53	-1.69E-46
MS.gene057890	MS.gene34078	-0.808052	3.74E-50	-1.69E-46
MS.gene057919	MS.gene34078	0.821443	4.03E-53	-1.69E-46
MS.gene057999	MS.gene34078	0.825567	4.37E-54	-1.69E-46
MS.gene058008	MS.gene34078	0.803682	3.10E-49	-1.69E-46
MS.gene058159	MS.gene34078	0.832362	9.87E-56	-1.69E-46
MS.gene058279	MS.gene34078	0.841414	4.82E-58	-1.69E-46
MS.gene058592	MS.gene34078	0.854223	1.42E-61	-1.69E-46
MS.gene058781	MS.gene34078	0.807632	4.60E-50	-1.69E-46
MS.gene05878	MS.gene34078	0.802574	5.26E-49	-1.69E-46
MS.gene059146	MS.gene34078	0.821364	4.20E-53	-1.69E-46
MS.gene05952	MS.gene34078	0.839437	1.59E-57	-1.69E-46
MS.gene05985	MS.gene34078	-0.870767	1.14E-66	-1.69E-46
MS.gene059901	MS.gene34078	-0.84635	2.30E-59	-1.69E-46
MS.gene059965	MS.gene34078	0.817824	2.70E-52	-1.69E-46
MS.gene06011	MS.gene34078	0.818567	1.83E-52	-1.69E-46
MS.gene060244	MS.gene34078	0.808473	3.04E-50	-1.69E-46
MS.gene060259	MS.gene34078	0.800527	1.38E-48	-1.69E-46
MS.gene060338	MS.gene34078	0.80233	5.90E-49	-1.69E-46
MS.gene060365	MS.gene34078	0.822976	1.78E-53	-1.69E-46
MS.gene060412	MS.gene34078	0.80473	1.88E-49	-1.69E-46
MS.gene060435	MS.gene34078	0.804174	2.45E-49	-1.69E-46
MS.gene060527	MS.gene34078	0.810831	9.48E-51	-1.69E-46
MS.gene060763	MS.gene34078	0.82484	6.49E-54	-1.69E-46
MS.gene060933	MS.gene34078	0.830477	2.87E-55	-1.69E-46
MS.gene060935	MS.gene34078	0.841804	3.80E-58	-1.69E-46
MS.gene060937	MS.gene34078	0.858511	7.83E-63	-1.69E-46
MS.gene061100	MS.gene34078	0.807934	3.96E-50	-1.69E-46
MS.gene061188	MS.gene34078	0.816168	6.35E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34078.t1	MTR_1g094215	98.953	191	2	0	1	191	257	447	1.51e-133	381
MS.gene34078.t1	MTR_1g094215	35.000	120	78	0	19	138	193	312	8.34e-13	66.2
MS.gene34078.t1	MTR_1g094215	98.953	191	2	0	1	191	440	630	1.24e-131	383
MS.gene34078.t1	MTR_1g094215	35.000	120	78	0	19	138	376	495	4.91e-13	67.0
MS.gene34078.t1	MTR_4g063800	61.290	186	72	0	1	186	448	633	6.32e-72	229
MS.gene34078.t1	MTR_4g063800	34.167	120	79	0	19	138	384	503	4.98e-14	70.1
MS.gene34078.t1	MTR_4g485520	57.377	183	78	0	1	183	300	482	3.23e-68	216
MS.gene34078.t1	MTR_4g485520	31.655	139	94	1	1	138	217	355	5.35e-14	69.7
MS.gene34078.t1	MTR_4g485520	57.377	183	78	0	1	183	468	650	1.16e-66	215
MS.gene34078.t1	MTR_4g485520	31.667	120	82	0	19	138	404	523	3.62e-14	70.5
MS.gene34078.t1	MTR_7g059405	53.631	179	81	1	1	179	456	632	3.01e-53	179
MS.gene34078.t1	MTR_7g078330	49.171	181	92	0	3	183	464	644	1.47e-46	162
MS.gene34078.t1	MTR_3g466220	55.429	175	77	1	2	176	649	822	2.20e-45	159
MS.gene34078.t1	MTR_3g466220	31.624	117	80	0	19	135	584	700	4.34e-12	64.3
MS.gene34078.t1	MTR_3g466220	55.429	175	77	1	2	176	628	801	4.14e-45	159
MS.gene34078.t1	MTR_3g466220	31.624	117	80	0	19	135	563	679	5.75e-12	63.9
MS.gene34078.t1	MTR_3g103050	45.251	179	98	0	3	181	152	330	4.32e-45	152
MS.gene34078.t1	MTR_1g018180	42.458	179	103	0	3	181	158	336	8.57e-43	146
MS.gene34078.t1	MTR_1g018180	29.752	121	85	0	19	139	92	212	3.53e-12	64.3
MS.gene34078.t1	MTR_5g083030	43.784	185	100	3	1	181	501	685	4.39e-41	147
MS.gene34078.t1	MTR_1g069845	47.701	174	90	1	3	176	420	592	4.32e-40	144
MS.gene34078.t1	MTR_1g069845	30.147	136	94	1	1	135	335	470	3.63e-12	64.7
MS.gene34078.t1	MTR_7g097020	48.276	174	89	1	3	176	579	751	8.52e-40	144
MS.gene34078.t1	MTR_7g097020	31.111	135	92	1	2	135	495	629	2.38e-13	68.2
MS.gene34078.t1	MTR_1g069845	47.701	174	90	1	3	176	537	709	1.17e-39	143
MS.gene34078.t1	MTR_1g069845	30.147	136	94	1	1	135	452	587	5.31e-12	63.9
MS.gene34078.t1	MTR_1g069845	47.701	174	90	1	3	176	541	713	1.25e-39	143
MS.gene34078.t1	MTR_1g069845	30.147	136	94	1	1	135	456	591	5.69e-12	63.9
MS.gene34078.t1	MTR_7g053260	42.391	184	102	3	1	182	271	452	1.42e-37	135
MS.gene34078.t1	MTR_1g093995	83.099	71	12	0	39	109	126	196	2.11e-36	125
MS.gene34078.t1	MTR_1g093965	83.099	71	12	0	39	109	126	196	2.21e-36	125
MS.gene34078.t1	MTR_2g026130	42.162	185	107	0	3	187	272	456	9.27e-36	130
MS.gene34078.t1	MTR_2g026130	33.058	121	81	0	19	139	206	326	1.41e-12	65.5
MS.gene34078.t1	MTR_1g094025	65.625	96	10	1	1	96	207	279	1.79e-29	110
MS.gene34078.t1	MTR_2g018010	41.711	187	103	4	2	184	494	678	2.24e-29	114
MS.gene34078.t1	MTR_7g116600	38.798	183	108	3	1	181	272	452	1.94e-28	110
MS.gene34078.t1	MTR_3g078340	38.462	182	107	2	2	180	307	486	3.30e-27	107
MS.gene34078.t1	MTR_4g091880	34.239	184	109	4	2	180	163	339	6.04e-21	88.6
MS.gene34078.t1	MTR_4g091880	34.239	184	109	4	2	180	191	367	6.82e-21	89.0
MS.gene34078.t1	MTR_1g079450	38.298	188	110	4	1	184	256	441	1.96e-20	88.2
MS.gene34078.t1	MTR_3g078160	25.773	194	138	4	1	188	472	665	8.38e-18	80.9
MS.gene34078.t1	MTR_3g078160	25.773	194	138	4	1	188	472	665	1.01e-17	80.5
MS.gene34078.t1	MTR_5g069340	33.679	193	122	4	4	191	133	324	1.93e-17	79.0
MS.gene34078.t1	MTR_4g107010	31.183	186	122	4	1	180	479	664	3.09e-17	79.3
MS.gene34078.t1	MTR_6g008170	31.461	178	117	2	2	176	334	509	9.86e-17	77.8
MS.gene34078.t1	MTR_3g067685	29.518	166	111	4	20	180	154	318	1.57e-14	71.2
MS.gene34078.t1	MTR_3g106140	30.159	189	122	4	5	185	213	399	2.54e-14	70.9
MS.gene34078.t1	MTR_5g079550	27.363	201	129	6	4	190	117	314	1.40e-13	68.2
MS.gene34078.t1	MTR_1g014420	29.630	189	123	4	5	185	232	418	1.76e-13	68.2
MS.gene34078.t1	MTR_5g034440	29.947	187	124	4	1	180	479	665	4.59e-13	67.0
MS.gene34078.t1	MTR_3g085610	29.189	185	124	4	7	186	531	713	1.40e-12	65.9
MS.gene34078.t1	MTR_2g007630	47.826	69	35	1	1	68	126	194	5.43e-12	63.2
MS.gene34078.t1	MTR_2g011140	30.387	181	112	5	7	180	206	379	6.50e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34078.t1	AT3G54850	75.543	184	45	0	1	184	442	625	3.61e-85	263
MS.gene34078.t1	AT3G54850	33.333	120	80	0	19	138	378	497	2.13e-12	65.1
MS.gene34078.t1	AT3G46510	63.187	182	67	0	1	182	449	630	2.02e-71	228
MS.gene34078.t1	AT3G46510	33.333	120	80	0	19	138	385	504	1.16e-12	65.9
MS.gene34078.t1	AT2G28830	56.757	185	80	0	1	185	382	566	5.03e-68	218
MS.gene34078.t1	AT2G28830	34.959	123	78	2	18	138	315	437	1.09e-12	66.2
MS.gene34078.t1	AT1G71020	57.692	182	76	1	1	181	291	472	2.88e-62	200
MS.gene34078.t1	AT1G71020	32.857	140	92	2	1	138	207	346	1.40e-13	68.6
MS.gene34078.t1	AT1G23030	57.143	182	77	1	1	181	428	609	1.16e-61	201
MS.gene34078.t1	AT1G23030	32.609	138	92	1	2	138	346	483	6.74e-16	75.5
MS.gene34078.t1	AT1G71020	57.692	182	76	1	1	181	439	620	7.77e-61	199
MS.gene34078.t1	AT1G71020	32.857	140	92	2	1	138	355	494	1.44e-13	68.6
MS.gene34078.t1	AT2G23140	52.514	179	84	1	2	180	450	627	5.47e-48	165
MS.gene34078.t1	AT2G23140	31.624	117	80	0	19	135	385	501	1.43e-12	65.9
MS.gene34078.t1	AT2G23140	52.514	179	84	1	2	180	450	627	5.47e-48	165
MS.gene34078.t1	AT2G23140	31.624	117	80	0	19	135	385	501	1.43e-12	65.9
MS.gene34078.t1	AT2G23140	52.514	179	84	1	2	180	642	819	1.78e-47	166
MS.gene34078.t1	AT2G23140	31.624	117	80	0	19	135	577	693	9.44e-13	66.2
MS.gene34078.t1	AT2G23140	52.514	179	84	1	2	180	639	816	1.80e-47	166
MS.gene34078.t1	AT2G23140	31.624	117	80	0	19	135	574	690	9.25e-13	66.2
MS.gene34078.t1	AT2G23140	52.514	179	84	1	2	180	639	816	1.80e-47	166
MS.gene34078.t1	AT2G23140	31.624	117	80	0	19	135	574	690	9.25e-13	66.2
MS.gene34078.t1	AT5G42340	51.099	182	89	0	2	183	477	658	1.41e-44	156
MS.gene34078.t1	AT3G01400	43.017	179	102	0	3	181	162	340	9.02e-44	149
MS.gene34078.t1	AT3G01400	31.148	122	84	0	19	140	96	217	2.62e-13	67.4
MS.gene34078.t1	AT5G67340	45.251	179	97	1	2	180	520	697	3.91e-38	139
MS.gene34078.t1	AT5G67340	33.058	121	74	2	19	135	454	571	8.07e-11	60.8
MS.gene34078.t1	AT5G67340	45.251	179	97	1	2	180	520	697	3.91e-38	139
MS.gene34078.t1	AT5G67340	33.058	121	74	2	19	135	454	571	8.07e-11	60.8
MS.gene34078.t1	AT5G58680	38.547	179	109	1	4	181	161	339	1.80e-36	130
MS.gene34078.t1	AT5G58680	26.829	123	90	0	19	141	94	216	6.60e-11	60.5
MS.gene34078.t1	AT3G54790	48.000	175	89	2	3	176	535	708	2.32e-36	134
MS.gene34078.t1	AT3G54790	48.000	175	89	2	3	176	571	744	7.58e-36	132
MS.gene34078.t1	AT3G54790	48.000	175	89	2	3	176	571	744	7.58e-36	132
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	139	317	2.69e-33	121
MS.gene34078.t1	AT3G07360	40.884	181	103	3	3	181	274	452	2.57e-32	120
MS.gene34078.t1	AT4G16490	42.246	187	106	1	3	187	282	468	1.10e-30	116
MS.gene34078.t1	AT4G16490	33.884	121	80	0	19	139	216	336	6.80e-11	60.8
MS.gene34078.t1	AT5G62560	37.778	180	105	3	2	176	332	509	2.47e-24	99.8
MS.gene34078.t1	AT1G29340	39.572	187	107	4	1	183	505	689	5.99e-23	95.9
MS.gene34078.t1	AT5G65200	35.714	196	113	3	2	186	322	515	1.12e-22	94.7
MS.gene34078.t1	AT5G18320	34.574	188	111	5	2	182	267	449	2.73e-21	90.5
MS.gene34078.t1	AT5G40140	33.846	195	122	4	1	190	325	517	1.56e-19	85.9
MS.gene34078.t1	AT4G12710	33.684	190	121	4	4	188	149	338	3.12e-19	84.7
MS.gene34078.t1	AT4G31890	32.653	196	124	4	2	191	246	439	8.62e-18	80.9
MS.gene34078.t1	AT4G31890	32.653	196	124	4	2	191	246	439	8.62e-18	80.9
MS.gene34078.t1	AT4G31890	32.653	196	124	4	2	191	246	439	8.62e-18	80.9
MS.gene34078.t1	AT3G47820	34.759	187	112	5	2	180	303	487	1.26e-16	77.4
MS.gene34078.t1	AT1G67530	29.670	182	125	3	2	180	525	706	4.12e-16	76.3
MS.gene34078.t1	AT1G67530	29.670	182	125	3	2	180	525	706	4.12e-16	76.3
MS.gene34078.t1	AT1G67530	29.670	182	125	3	2	180	525	706	4.12e-16	76.3
MS.gene34078.t1	AT2G25130	33.155	187	113	5	5	183	216	398	4.01e-15	73.2
MS.gene34078.t1	AT3G03440	29.474	190	127	4	4	187	164	352	2.21e-13	67.8
MS.gene34078.t1	AT1G24330	26.923	182	131	2	7	186	530	711	3.30e-13	67.8
MS.gene34078.t1	AT1G24330	26.923	182	131	2	7	186	530	711	3.30e-13	67.8
MS.gene34078.t1	AT5G01830	32.487	197	117	6	2	191	467	654	2.49e-11	62.0

Find 47 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTATAACCTCAACTAAAAT+AGG	0.132718	1.2:-74277636	None:intergenic
CTTCAGGGATTATCAGAAAA+TGG	0.310426	1.2:+74277768	MS.gene34078:CDS
CCTTTGCTAGTTTCAATTGC+AGG	0.330384	1.2:-74277725	None:intergenic
TGGAGCAGAAGAAGCACTTC+AGG	0.351787	1.2:+74277752	MS.gene34078:CDS
GCTATAATGACAATCCTTGC+AGG	0.409994	1.2:+74277570	MS.gene34078:CDS
CTCACGGTTGCGTGGTGATC+CGG	0.413042	1.2:-74277661	None:intergenic
CAAAGTAACTTTATAAGAGC+CGG	0.431128	1.2:-74277390	None:intergenic
TCCTTTGATACGATTTATGA+AGG	0.431714	1.2:+74277515	MS.gene34078:CDS
GATAGAGCTAAGAGAAAAGC+TGG	0.432820	1.2:+74277795	MS.gene34078:CDS
TTTAATCTATGTATTTATCA+AGG	0.434426	1.2:+74277456	MS.gene34078:CDS
GATGAGAACAAAGTTGCAAT+AGG	0.435327	1.2:+74277351	MS.gene34078:CDS
TTAGTTGAGGTTATACGAAC+CGG	0.456126	1.2:+74277642	MS.gene34078:CDS
TTGCTGTCCTTCCTTCATGA+TGG	0.458588	1.2:-74277593	None:intergenic
GTATAACCTCAACTAAAATA+GGG	0.461180	1.2:-74277635	None:intergenic
TTCCTTCATGATGGCCTGCA+AGG	0.462779	1.2:-74277584	None:intergenic
ACCTCAACTAAAATAGGGAT+TGG	0.470796	1.2:-74277630	None:intergenic
GAAGGATGCGGGCGGTGGGA+TGG	0.485190	1.2:+74277533	MS.gene34078:CDS
CATGAAGGAAGGACAGCAAT+TGG	0.491237	1.2:+74277597	MS.gene34078:CDS
AAAGTTGCAATAGGTGCAGC+AGG	0.503040	1.2:+74277360	MS.gene34078:CDS
TTTATGAAGGATGCGGGCGG+TGG	0.504174	1.2:+74277528	MS.gene34078:CDS
GATACGATTTATGAAGGATG+CGG	0.516210	1.2:+74277521	MS.gene34078:CDS
AAAGCTAGAGCTGTTAAAGC+CGG	0.526299	1.2:+74277483	MS.gene34078:CDS
CTTATAAAGTTACTTTGTGA+AGG	0.532116	1.2:+74277396	MS.gene34078:CDS
AGGAAGGACAGCAATTGGTC+AGG	0.536014	1.2:+74277602	MS.gene34078:CDS
AGGTGCAGCAGGAGCCATCC+CGG	0.539324	1.2:+74277371	MS.gene34078:CDS
GAACTGTTGCAGCGAATCGA+AGG	0.545611	1.2:+74277828	MS.gene34078:CDS
ATACGATTTATGAAGGATGC+GGG	0.548943	1.2:+74277522	MS.gene34078:CDS
AAAGTAACTTTATAAGAGCC+GGG	0.549044	1.2:-74277389	None:intergenic
GGAGCAGAAGAAGCACTTCA+GGG	0.556560	1.2:+74277753	MS.gene34078:CDS
AAGCTAGAGCTGTTAAAGCC+GGG	0.558896	1.2:+74277484	MS.gene34078:CDS
TTATGAAGGATGCGGGCGGT+GGG	0.567221	1.2:+74277529	MS.gene34078:CDS
AACTGTTGCAGCGAATCGAA+GGG	0.569767	1.2:+74277829	MS.gene34078:CDS
ATCCTTGCAGGCCATCATGA+AGG	0.571335	1.2:+74277582	MS.gene34078:CDS
TCCTTCATAAATCGTATCAA+AGG	0.578982	1.2:-74277516	None:intergenic
TATCAAAGGAGCAACTATCC+CGG	0.586650	1.2:-74277502	None:intergenic
TTGAAACTAGCAAAGGAACA+TGG	0.590002	1.2:+74277732	MS.gene34078:CDS
AACTAAAATAGGGATTGGCT+CGG	0.594552	1.2:-74277625	None:intergenic
TAACTTTATAAGAGCCGGGA+TGG	0.597754	1.2:-74277385	None:intergenic
GCAGCATTCTCACGGTTGCG+TGG	0.609043	1.2:-74277669	None:intergenic
TTGCAGGCCATCATGAAGGA+AGG	0.615941	1.2:+74277586	MS.gene34078:CDS
CCTGCAATTGAAACTAGCAA+AGG	0.625998	1.2:+74277725	MS.gene34078:CDS
AAACAGCAGCAGCATTCTCA+CGG	0.633676	1.2:-74277677	None:intergenic
CTTTGTGAAGGTACTCCAAG+AGG	0.641917	1.2:+74277408	MS.gene34078:CDS
CATGGAGGCGAGAGAAAACG+CGG	0.648697	1.2:+74277299	None:intergenic
GTTGCAGCGAATCGAAGGGG+AGG	0.677232	1.2:+74277833	MS.gene34078:CDS
ACTGTTGCAGCGAATCGAAG+GGG	0.696214	1.2:+74277830	MS.gene34078:CDS
CGATTTATGAAGGATGCGGG+CGG	0.720329	1.2:+74277525	MS.gene34078:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTAATCTATGTATTTATCA+AGG	+	chr1.2:74277456-74277475	MS.gene34078:CDS	15.0%
!	GTATAACCTCAACTAAAATA+GGG	-	chr1.2:74277638-74277657	None:intergenic	25.0%
!!	CTTATAAAGTTACTTTGTGA+AGG	+	chr1.2:74277396-74277415	MS.gene34078:CDS	25.0%
	AAAGTAACTTTATAAGAGCC+GGG	-	chr1.2:74277392-74277411	None:intergenic	30.0%
	CAAAGTAACTTTATAAGAGC+CGG	-	chr1.2:74277393-74277412	None:intergenic	30.0%
	CGTATAACCTCAACTAAAAT+AGG	-	chr1.2:74277639-74277658	None:intergenic	30.0%
	TCCTTCATAAATCGTATCAA+AGG	-	chr1.2:74277519-74277538	None:intergenic	30.0%
	TCCTTTGATACGATTTATGA+AGG	+	chr1.2:74277515-74277534	MS.gene34078:CDS	30.0%
	ACCTCAACTAAAATAGGGAT+TGG	-	chr1.2:74277633-74277652	None:intergenic	35.0%
	ATACGATTTATGAAGGATGC+GGG	+	chr1.2:74277522-74277541	MS.gene34078:CDS	35.0%
	CTTCAGGGATTATCAGAAAA+TGG	+	chr1.2:74277768-74277787	MS.gene34078:CDS	35.0%
	GATACGATTTATGAAGGATG+CGG	+	chr1.2:74277521-74277540	MS.gene34078:CDS	35.0%
	GATGAGAACAAAGTTGCAAT+AGG	+	chr1.2:74277351-74277370	MS.gene34078:CDS	35.0%
	TTAGTTGAGGTTATACGAAC+CGG	+	chr1.2:74277642-74277661	MS.gene34078:CDS	35.0%
	TTGAAACTAGCAAAGGAACA+TGG	+	chr1.2:74277732-74277751	MS.gene34078:CDS	35.0%
!!	AACTAAAATAGGGATTGGCT+CGG	-	chr1.2:74277628-74277647	None:intergenic	35.0%
	AAAGCTAGAGCTGTTAAAGC+CGG	+	chr1.2:74277483-74277502	MS.gene34078:CDS	40.0%
	CCTGCAATTGAAACTAGCAA+AGG	+	chr1.2:74277725-74277744	MS.gene34078:CDS	40.0%
	GATAGAGCTAAGAGAAAAGC+TGG	+	chr1.2:74277795-74277814	MS.gene34078:CDS	40.0%
	GCTATAATGACAATCCTTGC+AGG	+	chr1.2:74277570-74277589	MS.gene34078:CDS	40.0%
	TAACTTTATAAGAGCCGGGA+TGG	-	chr1.2:74277388-74277407	None:intergenic	40.0%
	TATCAAAGGAGCAACTATCC+CGG	-	chr1.2:74277505-74277524	None:intergenic	40.0%
!	CCTTTGCTAGTTTCAATTGC+AGG	-	chr1.2:74277728-74277747	None:intergenic	40.0%
!!	TGTTTTGTGGTTGGTATGCA+CGG	+	chr1.2:74277695-74277714	MS.gene34078:CDS	40.0%
!!!	GCCAATCCCTATTTTAGTTG+AGG	+	chr1.2:74277629-74277648	MS.gene34078:CDS	40.0%
	AAACAGCAGCAGCATTCTCA+CGG	-	chr1.2:74277680-74277699	None:intergenic	45.0%
	AAAGTTGCAATAGGTGCAGC+AGG	+	chr1.2:74277360-74277379	MS.gene34078:CDS	45.0%
	AACTGTTGCAGCGAATCGAA+GGG	+	chr1.2:74277829-74277848	MS.gene34078:CDS	45.0%
	AAGCTAGAGCTGTTAAAGCC+GGG	+	chr1.2:74277484-74277503	MS.gene34078:CDS	45.0%
	CATGAAGGAAGGACAGCAAT+TGG	+	chr1.2:74277597-74277616	MS.gene34078:CDS	45.0%
	TTGCTGTCCTTCCTTCATGA+TGG	-	chr1.2:74277596-74277615	None:intergenic	45.0%
!!	CTTTGTGAAGGTACTCCAAG+AGG	+	chr1.2:74277408-74277427	MS.gene34078:CDS	45.0%
!!	GTTTTGTGGTTGGTATGCAC+GGG	+	chr1.2:74277696-74277715	MS.gene34078:CDS	45.0%
!!!	AGAATGCTGCTGCTGTTTTG+TGG	+	chr1.2:74277682-74277701	MS.gene34078:CDS	45.0%
!!!	GCTGCATCTTTTTTGCCTCT+TGG	-	chr1.2:74277426-74277445	None:intergenic	45.0%
	ACTGTTGCAGCGAATCGAAG+GGG	+	chr1.2:74277830-74277849	MS.gene34078:CDS	50.0%
	AGGAAGGACAGCAATTGGTC+AGG	+	chr1.2:74277602-74277621	MS.gene34078:CDS	50.0%
	ATCCTTGCAGGCCATCATGA+AGG	+	chr1.2:74277582-74277601	MS.gene34078:CDS	50.0%
	CGATTTATGAAGGATGCGGG+CGG	+	chr1.2:74277525-74277544	MS.gene34078:CDS	50.0%
	GAACTGTTGCAGCGAATCGA+AGG	+	chr1.2:74277828-74277847	MS.gene34078:CDS	50.0%
	TTCCTTCATGATGGCCTGCA+AGG	-	chr1.2:74277587-74277606	None:intergenic	50.0%
	TTGCAGGCCATCATGAAGGA+AGG	+	chr1.2:74277586-74277605	MS.gene34078:CDS	50.0%
!!	GGAGCAGAAGAAGCACTTCA+GGG	+	chr1.2:74277753-74277772	MS.gene34078:CDS	50.0%
!!	TGGAGCAGAAGAAGCACTTC+AGG	+	chr1.2:74277752-74277771	MS.gene34078:CDS	50.0%
!!!	TGCTGCTGCTGTTTTGTGGT+TGG	+	chr1.2:74277686-74277705	MS.gene34078:CDS	50.0%
	TTATGAAGGATGCGGGCGGT+GGG	+	chr1.2:74277529-74277548	MS.gene34078:CDS	55.0%
	TTTATGAAGGATGCGGGCGG+TGG	+	chr1.2:74277528-74277547	MS.gene34078:CDS	55.0%
	CTCACGGTTGCGTGGTGATC+CGG	-	chr1.2:74277664-74277683	None:intergenic	60.0%
	GCAGCATTCTCACGGTTGCG+TGG	-	chr1.2:74277672-74277691	None:intergenic	60.0%
	GTTGCAGCGAATCGAAGGGG+AGG	+	chr1.2:74277833-74277852	MS.gene34078:CDS	60.0%
	AGGTGCAGCAGGAGCCATCC+CGG	+	chr1.2:74277371-74277390	MS.gene34078:CDS	65.0%
	GAAGGATGCGGGCGGTGGGA+TGG	+	chr1.2:74277533-74277552	MS.gene34078:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	74277300	74277875	74277300	ID=MS.gene34078
chr1.2	mRNA	74277300	74277875	74277300	ID=MS.gene34078.t1;Parent=MS.gene34078
chr1.2	exon	74277300	74277875	74277300	ID=MS.gene34078.t1.exon1;Parent=MS.gene34078.t1
chr1.2	CDS	74277300	74277875	74277300	ID=cds.MS.gene34078.t1;Parent=MS.gene34078.t1

Gene Sequence

>MS.gene34078

ATGGAGGCGAGAGAAAACGCGGCTGCGACTCTCTTTAGTTTATCAGTTCTTGATGAGAACAAAGTTGCAATAGGTGCAGCAGGAGCCATCCCGGCTCTTATAAAGTTACTTTGTGAAGGTACTCCAAGAGGCAAAAAAGATGCAGCTACTGCTATATTTAATCTATGTATTTATCAAGGAAACAAAGCTAGAGCTGTTAAAGCCGGGATAGTTGCTCCTTTGATACGATTTATGAAGGATGCGGGCGGTGGGATGGTTGACGAAGCACTAGCTATAATGACAATCCTTGCAGGCCATCATGAAGGAAGGACAGCAATTGGTCAGGCCGAGCCAATCCCTATTTTAGTTGAGGTTATACGAACCGGATCACCACGCAACCGTGAGAATGCTGCTGCTGTTTTGTGGTTGGTATGCACGGGAGATCTCCTGCAATTGAAACTAGCAAAGGAACATGGAGCAGAAGAAGCACTTCAGGGATTATCAGAAAATGGAACTGATAGAGCTAAGAGAAAAGCTGGAAGTATGTTAGAACTGTTGCAGCGAATCGAAGGGGAGGATAGTTTGCAAAATACCTAG

Protein sequence

>MS.gene34078.t1

MEARENAAATLFSLSVLDENKVAIGAAGAIPALIKLLCEGTPRGKKDAATAIFNLCIYQGNKARAVKAGIVAPLIRFMKDAGGGMVDEALAIMTILAGHHEGRTAIGQAEPIPILVEVIRTGSPRNRENAAAVLWLVCTGDLLQLKLAKEHGAEEALQGLSENGTDRAKRKAGSMLELLQRIEGEDSLQNT