AlfalfaGEDB | MS.gene34310

M. sativa cultivar XinJiangDaYe / MS.gene34310

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34310.t1	XP_013454491.1	99	98	1	0	1	98	81	178	2.40E-48	201.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34310.t1	A0A072UFR3	99.0	98	1	0	1	98	81	178	1.8e-48	201.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene34310	0.81251	4.09E-51	-1.69E-46
MS.gene049171	MS.gene34310	0.819622	1.05E-52	-1.69E-46
MS.gene049173	MS.gene34310	0.804122	2.51E-49	-1.69E-46
MS.gene049222	MS.gene34310	0.826938	2.06E-54	-1.69E-46
MS.gene049427	MS.gene34310	0.827151	1.83E-54	-1.69E-46
MS.gene049609	MS.gene34310	0.820006	8.61E-53	-1.69E-46
MS.gene049633	MS.gene34310	0.813877	2.05E-51	-1.69E-46
MS.gene049805	MS.gene34310	0.811325	7.41E-51	-1.69E-46
MS.gene050538	MS.gene34310	0.807422	5.09E-50	-1.69E-46
MS.gene050747	MS.gene34310	0.83015	3.45E-55	-1.69E-46
MS.gene050772	MS.gene34310	0.801303	9.60E-49	-1.69E-46
MS.gene05080	MS.gene34310	0.814068	1.86E-51	-1.69E-46
MS.gene05081	MS.gene34310	0.812336	4.46E-51	-1.69E-46
MS.gene050950	MS.gene34310	0.827996	1.15E-54	-1.69E-46
MS.gene051617	MS.gene34310	0.803146	4.01E-49	-1.69E-46
MS.gene051666	MS.gene34310	0.847285	1.27E-59	-1.69E-46
MS.gene051838	MS.gene34310	0.843404	1.43E-58	-1.69E-46
MS.gene052481	MS.gene34310	0.800374	1.49E-48	-1.69E-46
MS.gene052484	MS.gene34310	0.801797	7.60E-49	-1.69E-46
MS.gene052501	MS.gene34310	0.827651	1.39E-54	-1.69E-46
MS.gene052503	MS.gene34310	0.82326	1.52E-53	-1.69E-46
MS.gene052505	MS.gene34310	0.810571	1.08E-50	-1.69E-46
MS.gene052823	MS.gene34310	0.80809	3.67E-50	-1.69E-46
MS.gene052825	MS.gene34310	0.81664	4.98E-52	-1.69E-46
MS.gene052870	MS.gene34310	0.806978	6.33E-50	-1.69E-46
MS.gene052872	MS.gene34310	0.81101	8.67E-51	-1.69E-46
MS.gene053103	MS.gene34310	0.810248	1.27E-50	-1.69E-46
MS.gene053104	MS.gene34310	0.809333	1.99E-50	-1.69E-46
MS.gene053107	MS.gene34310	0.805466	1.32E-49	-1.69E-46
MS.gene053203	MS.gene34310	0.803261	3.79E-49	-1.69E-46
MS.gene053207	MS.gene34310	0.846807	1.72E-59	-1.69E-46
MS.gene053213	MS.gene34310	0.809458	1.87E-50	-1.69E-46
MS.gene053286	MS.gene34310	0.808655	2.78E-50	-1.69E-46
MS.gene053480	MS.gene34310	0.805549	1.27E-49	-1.69E-46
MS.gene053632	MS.gene34310	0.837096	6.36E-57	-1.69E-46
MS.gene053824	MS.gene34310	0.812932	3.30E-51	-1.69E-46
MS.gene053916	MS.gene34310	0.80363	3.18E-49	-1.69E-46
MS.gene054600	MS.gene34310	0.845125	4.94E-59	-1.69E-46
MS.gene054642	MS.gene34310	0.811155	8.06E-51	-1.69E-46
MS.gene05470	MS.gene34310	0.811658	6.27E-51	-1.69E-46
MS.gene054927	MS.gene34310	0.823571	1.29E-53	-1.69E-46
MS.gene055206	MS.gene34310	0.834652	2.65E-56	-1.69E-46
MS.gene055289	MS.gene34310	0.83268	8.23E-56	-1.69E-46
MS.gene055502	MS.gene34310	0.80009	1.70E-48	-1.69E-46
MS.gene055577	MS.gene34310	0.832906	7.23E-56	-1.69E-46
MS.gene055748	MS.gene34310	0.803817	2.91E-49	-1.69E-46
MS.gene055941	MS.gene34310	0.813824	2.10E-51	-1.69E-46
MS.gene056897	MS.gene34310	0.8202	7.78E-53	-1.69E-46
MS.gene057124	MS.gene34310	0.811226	7.78E-51	-1.69E-46
MS.gene057196	MS.gene34310	0.810842	9.43E-51	-1.69E-46
MS.gene057332	MS.gene34310	0.806232	9.09E-50	-1.69E-46
MS.gene057416	MS.gene34310	0.813289	2.76E-51	-1.69E-46
MS.gene057658	MS.gene34310	0.852882	3.45E-61	-1.69E-46
MS.gene058419	MS.gene34310	0.815787	7.73E-52	-1.69E-46
MS.gene058544	MS.gene34310	0.827855	1.24E-54	-1.69E-46
MS.gene058838	MS.gene34310	0.823257	1.53E-53	-1.69E-46
MS.gene059023	MS.gene34310	0.835765	1.39E-56	-1.69E-46
MS.gene05914	MS.gene34310	0.82576	3.93E-54	-1.69E-46
MS.gene05934	MS.gene34310	0.812654	3.80E-51	-1.69E-46
MS.gene059677	MS.gene34310	0.808545	2.94E-50	-1.69E-46
MS.gene059718	MS.gene34310	0.817878	2.62E-52	-1.69E-46
MS.gene060762	MS.gene34310	0.824469	7.94E-54	-1.69E-46
MS.gene060835	MS.gene34310	0.812553	4.00E-51	-1.69E-46
MS.gene06092	MS.gene34310	0.858106	1.03E-62	-1.69E-46
MS.gene061150	MS.gene34310	0.825587	4.32E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTTGCTGTTCTTGACAATT+TGG	0.246299	4.3:+89141532	MS.gene34310:CDS
ATCTCATCCACTTGTATATC+AGG	0.333453	4.3:-89141067	None:intergenic
TGATGATTTCAGAAGGTTCA+TGG	0.389939	4.3:+89141009	None:intergenic
ATGAACTTGTATCTCGAATC+TGG	0.411354	4.3:+89141510	MS.gene34310:CDS
TATTTGTTGACTTATATGTA+AGG	0.446098	4.3:-89141032	None:intergenic
ATACTTCACGGAACCATGAT+TGG	0.450822	4.3:+89141157	MS.gene34310:CDS
TACAAGTGGATGAGATTGTT+GGG	0.457723	4.3:+89141074	MS.gene34310:CDS
TCCACCATGAGGAGGCATAA+TGG	0.492329	4.3:-89141134	None:intergenic
GCAGATCAGTTTATTGCCAA+TGG	0.496622	4.3:+89141467	MS.gene34310:intron
AGATTCGAGATACAAGTTCA+TGG	0.497424	4.3:-89141508	None:intergenic
CCTCATGGTGGAATACTTCA+CGG	0.510920	4.3:+89141145	MS.gene34310:CDS
TACGTCTCTTGACTTGCCAT+TGG	0.513346	4.3:-89141483	None:intergenic
CTTAAACGCCTTGCACATGA+AGG	0.518985	4.3:+89141581	MS.gene34310:CDS
AAGGCGTTTAAGTAAGCAAA+AGG	0.541095	4.3:-89141570	None:intergenic
GGGGCATGCCATACCAATCA+TGG	0.551161	4.3:-89141170	None:intergenic
CTTTCCATTATGCCTCCTCA+TGG	0.553623	4.3:+89141130	MS.gene34310:CDS
ATACAAGTGGATGAGATTGT+TGG	0.562800	4.3:+89141073	MS.gene34310:CDS
GATTCGAGATACAAGTTCAT+GGG	0.566517	4.3:-89141507	None:intergenic
TTACATCTCCTTCATGTGCA+AGG	0.614326	4.3:-89141589	None:intergenic
TCCATTATGCCTCCTCATGG+TGG	0.618370	4.3:+89141133	MS.gene34310:CDS
TTCTGATCCTGATATACAAG+TGG	0.625145	4.3:+89141060	MS.gene34310:CDS
TGAAGTATTCCACCATGAGG+AGG	0.648479	4.3:-89141142	None:intergenic
CCGTGAAGTATTCCACCATG+AGG	0.714100	4.3:-89141145	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCATTTCAATTAGAATCAT+AGG	-	chr4.3:89141340-89141359	None:intergenic	20.0%
!!!	TATTTGTTGACTTATATGTA+AGG	-	chr4.3:89141035-89141054	None:intergenic	20.0%
!	TGTAACAACAAAAGTAAAGA+AGG	-	chr4.3:89141197-89141216	None:intergenic	25.0%
!!	AATGAATTAGTTCTCTAAGT+TGG	+	chr4.3:89141354-89141373	MS.gene34310:intron	25.0%
!!	CTTATATCTTTATGTCCTTT+TGG	+	chr4.3:89141217-89141236	MS.gene34310:intron	25.0%
	AACAACAAAAGTAAAGAAGG+TGG	-	chr4.3:89141194-89141213	None:intergenic	30.0%
	ACAACAAAAGTAAAGAAGGT+GGG	-	chr4.3:89141193-89141212	None:intergenic	30.0%
	AGGATCAATTCAGTTTGATA+TGG	-	chr4.3:89141320-89141339	None:intergenic	30.0%
	TTGGTTTATCTTGTTCTTGT+TGG	+	chr4.3:89141373-89141392	MS.gene34310:intron	30.0%
	ACTCGACATAAGAGTTGTTA+CGG	-	chr4.3:89141249-89141268	None:intergenic	35.0%
	AGATTCGAGATACAAGTTCA+TGG	-	chr4.3:89141511-89141530	None:intergenic	35.0%
	ATACAAGTGGATGAGATTGT+TGG	+	chr4.3:89141073-89141092	MS.gene34310:CDS	35.0%
	ATCTCATCCACTTGTATATC+AGG	-	chr4.3:89141070-89141089	None:intergenic	35.0%
	ATGAACTTGTATCTCGAATC+TGG	+	chr4.3:89141510-89141529	MS.gene34310:CDS	35.0%
	CAACAAAAGTAAAGAAGGTG+GGG	-	chr4.3:89141192-89141211	None:intergenic	35.0%
	GATTCGAGATACAAGTTCAT+GGG	-	chr4.3:89141510-89141529	None:intergenic	35.0%
	TACAAGTGGATGAGATTGTT+GGG	+	chr4.3:89141074-89141093	MS.gene34310:CDS	35.0%
	TTCTGATCCTGATATACAAG+TGG	+	chr4.3:89141060-89141079	MS.gene34310:CDS	35.0%
	TTGTAGGAGAGAGGTTAATA+TGG	+	chr4.3:89141270-89141289	MS.gene34310:intron	35.0%
	TTGTTACGGGATAAACCAAA+AGG	-	chr4.3:89141235-89141254	None:intergenic	35.0%
!	AAGGCGTTTAAGTAAGCAAA+AGG	-	chr4.3:89141573-89141592	None:intergenic	35.0%
!!	ACTCTTATGTCGAGTTTTGT+AGG	+	chr4.3:89141254-89141273	MS.gene34310:intron	35.0%
	ATACTTCACGGAACCATGAT+TGG	+	chr4.3:89141157-89141176	MS.gene34310:CDS	40.0%
	CTCGACATAAGAGTTGTTAC+GGG	-	chr4.3:89141248-89141267	None:intergenic	40.0%
	GCAGATCAGTTTATTGCCAA+TGG	+	chr4.3:89141467-89141486	MS.gene34310:intron	40.0%
!!	GCTTGCTGTTCTTGACAATT+TGG	+	chr4.3:89141532-89141551	MS.gene34310:CDS	40.0%
	CTTAAACGCCTTGCACATGA+AGG	+	chr4.3:89141581-89141600	MS.gene34310:CDS	45.0%
	CTTTCCATTATGCCTCCTCA+TGG	+	chr4.3:89141130-89141149	MS.gene34310:CDS	45.0%
	TCACGGAACCATGATTGGTA+TGG	+	chr4.3:89141162-89141181	MS.gene34310:intron	45.0%
!	CCTCATGGTGGAATACTTCA+CGG	+	chr4.3:89141145-89141164	MS.gene34310:CDS	45.0%
!	TGAAGTATTCCACCATGAGG+AGG	-	chr4.3:89141145-89141164	None:intergenic	45.0%
!!	TACGTCTCTTGACTTGCCAT+TGG	-	chr4.3:89141486-89141505	None:intergenic	45.0%
!!	TGTCGAGTTTTGTAGGAGAG+AGG	+	chr4.3:89141261-89141280	MS.gene34310:intron	45.0%
	CGCCCATAATCTAGCAAAGG+CGG	+	chr4.3:89141294-89141313	MS.gene34310:intron	50.0%
	GGCCGCCTTTGCTAGATTAT+GGG	-	chr4.3:89141299-89141318	None:intergenic	50.0%
	GGTCGCCCATAATCTAGCAA+AGG	+	chr4.3:89141291-89141310	MS.gene34310:intron	50.0%
	TCCATTATGCCTCCTCATGG+TGG	+	chr4.3:89141133-89141152	MS.gene34310:CDS	50.0%
	TGGCCGCCTTTGCTAGATTA+TGG	-	chr4.3:89141300-89141319	None:intergenic	50.0%
!	CCGTGAAGTATTCCACCATG+AGG	-	chr4.3:89141148-89141167	None:intergenic	50.0%
!	TCCACCATGAGGAGGCATAA+TGG	-	chr4.3:89141137-89141156	None:intergenic	50.0%
	GGGGCATGCCATACCAATCA+TGG	-	chr4.3:89141173-89141192	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	89141021	89141607	89141021	ID=MS.gene34310
chr4.3	mRNA	89141021	89141607	89141021	ID=MS.gene34310.t1;Parent=MS.gene34310
chr4.3	exon	89141021	89141178	89141021	ID=MS.gene34310.t1.exon1;Parent=MS.gene34310.t1
chr4.3	CDS	89141021	89141178	89141021	ID=cds.MS.gene34310.t1;Parent=MS.gene34310.t1
chr4.3	exon	89141471	89141607	89141471	ID=MS.gene34310.t1.exon2;Parent=MS.gene34310.t1
chr4.3	CDS	89141471	89141607	89141471	ID=cds.MS.gene34310.t1;Parent=MS.gene34310.t1

Gene Sequence

>MS.gene34310

AAGGTTCATGGCCTTACATATAAGTCAACAAATATTTGCTTCTGATCCTGATATACAAGTGGATGAGATTGTTGGGCATAGTTATCTGTTTTTGAAAGAGCAGCTTCAACTTTCCATTATGCCTCCTCATGGTGGAATACTTCACGGAACCATGATTGGTATGGCATGCCCCACCTTCTTTACTTTTGTTGTTACACTTATATCTTTATGTCCTTTTGGTTTATCCCGTAACAACTCTTATGTCGAGTTTTGTAGGAGAGAGGTTAATATGGTCGCCCATAATCTAGCAAAGGCGGCCATATCAAACTGAATTGATCCTATGATTCTAATTGAAATGAATTAGTTCTCTAAGTTGGTTTATCTTGTTCTTGTTGGATTATATTATTATTGTTAATATGTATATCAATAGTTATTAGAACTTTGATCATTCATTTTATTTTTATTTAGCAGATCAGTTTATTGCCAATGGCAAGTCAAGAGACGTAGCCCATGAACTTGTATCTCGAATCTGGCTTGCTGTTCTTGACAATTTGGAAGAAAATCAGCATACCTTTTGCTTACTTAAACGCCTTGCACATGAAGGAGAT

Protein sequence

>MS.gene34310.t1

KVHGLTYKSTNICFUSUYTSGUDCWAULSVFERAASTFHYASSWWNTSRNHDUSVYCQWQVKRRSPUTCISNLACCSUQFGRKSAYLLLTUTPCTURR