AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35799


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35799.t1 MTR_2g088720 96.212 132 5 0 1 132 1 132 2.65e-89 255
MS.gene35799.t1 MTR_2g088720 96.212 132 5 0 1 132 12 143 3.32e-89 255
MS.gene35799.t1 MTR_7g106600 83.077 130 22 0 4 133 1 130 1.43e-76 223
MS.gene35799.t1 MTR_7g106580 78.947 133 28 0 1 133 1 133 1.97e-73 215
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35799.t1 AT5G62980 63.492 126 45 1 5 129 4 129 5.87e-52 160
MS.gene35799.t1 AT5G62980 63.492 126 45 1 5 129 4 129 5.87e-52 160
MS.gene35799.t1 AT3G11750 62.810 121 44 1 10 129 21 141 9.45e-51 158
MS.gene35799.t1 AT3G21730 52.500 120 56 1 11 129 40 159 5.10e-41 134
MS.gene35799.t1 AT3G21730 46.032 63 33 1 68 129 13 75 1.50e-13 62.0

Find 0 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATAGCTAAGAAAGTTATTGA+AGG + 15127:11017-11036 MS.gene35799:CDS 25.0%
TAAAATTGCAATCGCTACTT+TGG + 15127:11073-11092 MS.gene35799:CDS 30.0%
! AAGTAGCGATTGCAATTTTA+TGG - 15127:11073-11092 None:intergenic 30.0%
! AGTAGCGATTGCAATTTTAT+GGG - 15127:11072-11091 None:intergenic 30.0%
! TTGACTTTCTATGGTTTTCA+CGG + 15127:10867-10886 MS.gene35799:CDS 30.0%
!! AAGGTGTTGATGCATTAATA+GGG + 15127:10820-10839 MS.gene35799:CDS 30.0%
GGAGACAAACTTATTCTAAG+GGG + 15127:10843-10862 MS.gene35799:CDS 35.0%
GGGAGACAAACTTATTCTAA+GGG + 15127:10842-10861 MS.gene35799:CDS 35.0%
! AAGTTATCAGATTTTCCAGC+TGG - 15127:10963-10982 None:intergenic 35.0%
!! AGGTGTTGATGCATTAATAG+GGG + 15127:10821-10840 MS.gene35799:CDS 35.0%
!! GAAGGTGTTGATGCATTAAT+AGG + 15127:10819-10838 MS.gene35799:CDS 35.0%
GGGGAGACAAACTTATTCTA+AGG + 15127:10841-10860 MS.gene35799:CDS 40.0%
TCAACGCCTAAGTAATCAAC+CGG - 15127:11164-11183 None:intergenic 40.0%
! CTAAGGGGATTGACTTTCTA+TGG + 15127:10858-10877 MS.gene35799:CDS 40.0%
!! GGTGTTGATGCATTAATAGG+GGG + 15127:10822-10841 MS.gene35799:CDS 40.0%
ACAGAAGTTTGTGGTAGACG+TGG + 15127:10917-10936 MS.gene35799:CDS 45.0%
GATATCTGCTGTTCGTGTGA+AGG + 15127:11109-11128 MS.gene35799:CDS 45.0%
! TGGTTTTCACGGAGCAATAG+CGG + 15127:10878-10897 MS.gene35799:CDS 45.0%
!! TCAAAACCTTCTGGAGTCAG+TGG + 15127:11046-11065 MS.gene35799:CDS 45.0%
AAAGCCTCATGTGGCGATTC+AGG + 15127:11136-11155 MS.gene35799:CDS 50.0%
AAGCCTCATGTGGCGATTCA+GGG + 15127:11137-11156 MS.gene35799:CDS 50.0%
AGGACCAGCTCAAAACCTTC+TGG + 15127:11037-11056 MS.gene35799:CDS 50.0%
CAGGGTCCGGTTGATTACTT+AGG + 15127:11155-11174 MS.gene35799:CDS 50.0%
GAAGGTTGGAAAGCCTCATG+TGG + 15127:11127-11146 MS.gene35799:CDS 50.0%
GACGCTAGGACAGAAGTTTG+TGG + 15127:10908-10927 MS.gene35799:CDS 50.0%
TCTGCTGTTCGTGTGAAGGT+TGG + 15127:11113-11132 MS.gene35799:CDS 50.0%
TGGTTGGATCTCAAACCAGC+TGG + 15127:10945-10964 MS.gene35799:CDS 50.0%
TTGTGGTAGACGTGGATGCT+TGG + 15127:10925-10944 MS.gene35799:CDS 50.0%
! ACGGAGCAATAGCGGAAGAA+AGG + 15127:10886-10905 MS.gene35799:CDS 50.0%
! ATAGCGGAAGAAAGGACGCT+AGG + 15127:10894-10913 MS.gene35799:CDS 50.0%
!! GTTGAGATTCTGAGACGCAG+AGG + 15127:11179-11198 MS.gene35799:CDS 50.0%
!!! GACTCCAGAAGGTTTTGAGC+TGG - 15127:11044-11063 None:intergenic 50.0%
GGTAGACGTGGATGCTTGGT+TGG + 15127:10929-10948 MS.gene35799:CDS 55.0%
TCATGTGGCGATTCAGGGTC+CGG + 15127:11142-11161 MS.gene35799:CDS 55.0%
!! TATGGGCCACTGACTCCAGA+AGG - 15127:11055-11074 None:intergenic 55.0%
GGACCCTGAATCGCCACATG+AGG - 15127:11143-11162 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
15127 gene 10816 11217 10816 ID=MS.gene35799
15127 mRNA 10816 11217 10816 ID=MS.gene35799.t1;Parent=MS.gene35799
15127 exon 10816 11217 10816 ID=MS.gene35799.t1.exon1;Parent=MS.gene35799.t1
15127 CDS 10816 11217 10816 ID=cds.MS.gene35799.t1;Parent=MS.gene35799.t1
Gene Sequence

>MS.gene35799

ATGGAAGGTGTTGATGCATTAATAGGGGGAGACAAACTTATTCTAAGGGGATTGACTTTCTATGGTTTTCACGGAGCAATAGCGGAAGAAAGGACGCTAGGACAGAAGTTTGTGGTAGACGTGGATGCTTGGTTGGATCTCAAACCAGCTGGAAAATCTGATAACTTGTCTGATTCTTTTAGTTACGCTGAAATATATCTTATAGCTAAGAAAGTTATTGAAGGACCAGCTCAAAACCTTCTGGAGTCAGTGGCCCATAAAATTGCAATCGCTACTTTGGAAGTTCAGAAAAAGATATCTGCTGTTCGTGTGAAGGTTGGAAAGCCTCATGTGGCGATTCAGGGTCCGGTTGATTACTTAGGCGTTGAGATTCTGAGACGCAGAGGCGATTTGACAGACTAG

Protein sequence

>MS.gene35799.t1

MEGVDALIGGDKLILRGLTFYGFHGAIAEERTLGQKFVVDVDAWLDLKPAGKSDNLSDSFSYAEIYLIAKKVIEGPAQNLLESVAHKIAIATLEVQKKISAVRVKVGKPHVAIQGPVDYLGVEILRRRGDLTD