AlfalfaGEDB | MS.gene38256

M. sativa cultivar XinJiangDaYe / MS.gene38256

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38256.t1	XP_003617884.1	100	117	0	0	1	117	30	146	8.50E-56	226.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38256.t1	P51414	87.2	117	15	0	1	117	30	146	1.5e-53	209.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38256.t1	G7K4S3	100.0	117	0	0	1	117	30	146	6.1e-56	226.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene38256	0.835122	2.02E-56	-1.69E-46
MS.gene049341	MS.gene38256	0.821363	4.20E-53	-1.69E-46
MS.gene049821	MS.gene38256	0.828898	6.96E-55	-1.69E-46
MS.gene049887	MS.gene38256	0.819026	1.44E-52	-1.69E-46
MS.gene050277	MS.gene38256	0.816115	6.53E-52	-1.69E-46
MS.gene050689	MS.gene38256	0.834709	2.56E-56	-1.69E-46
MS.gene050752	MS.gene38256	0.812828	3.48E-51	-1.69E-46
MS.gene050838	MS.gene38256	0.889696	1.92E-73	-1.69E-46
MS.gene051003	MS.gene38256	0.85103	1.16E-60	-1.69E-46
MS.gene051233	MS.gene38256	0.810477	1.13E-50	-1.69E-46
MS.gene051234	MS.gene38256	0.84568	3.49E-59	-1.69E-46
MS.gene051328	MS.gene38256	0.874189	8.20E-68	-1.69E-46
MS.gene051343	MS.gene38256	0.816108	6.55E-52	-1.69E-46
MS.gene05134	MS.gene38256	0.80134	9.43E-49	-1.69E-46
MS.gene051700	MS.gene38256	0.838477	2.81E-57	-1.69E-46
MS.gene051746	MS.gene38256	0.837736	4.36E-57	-1.69E-46
MS.gene051748	MS.gene38256	0.831369	1.74E-55	-1.69E-46
MS.gene051791	MS.gene38256	0.816089	6.62E-52	-1.69E-46
MS.gene051850	MS.gene38256	0.867651	1.17E-65	-1.69E-46
MS.gene051885	MS.gene38256	0.808888	2.48E-50	-1.69E-46
MS.gene05188	MS.gene38256	0.844914	5.63E-59	-1.69E-46
MS.gene051903	MS.gene38256	0.825963	3.52E-54	-1.69E-46
MS.gene051975	MS.gene38256	0.82945	5.11E-55	-1.69E-46
MS.gene052133	MS.gene38256	0.867155	1.69E-65	-1.69E-46
MS.gene052335	MS.gene38256	0.841314	5.12E-58	-1.69E-46
MS.gene052342	MS.gene38256	0.808398	3.16E-50	-1.69E-46
MS.gene052397	MS.gene38256	0.802293	6.01E-49	-1.69E-46
MS.gene052516	MS.gene38256	0.812466	4.18E-51	-1.69E-46
MS.gene052947	MS.gene38256	0.876966	9.17E-69	-1.69E-46
MS.gene053058	MS.gene38256	0.837469	5.10E-57	-1.69E-46
MS.gene053122	MS.gene38256	0.838472	2.82E-57	-1.69E-46
MS.gene053581	MS.gene38256	0.846421	2.20E-59	-1.69E-46
MS.gene054403	MS.gene38256	0.812795	3.54E-51	-1.69E-46
MS.gene054584	MS.gene38256	0.803096	4.10E-49	-1.69E-46
MS.gene055424	MS.gene38256	0.821448	4.02E-53	-1.69E-46
MS.gene055465	MS.gene38256	0.836018	1.20E-56	-1.69E-46
MS.gene055466	MS.gene38256	0.852986	3.22E-61	-1.69E-46
MS.gene055468	MS.gene38256	0.866828	2.14E-65	-1.69E-46
MS.gene055469	MS.gene38256	0.817965	2.51E-52	-1.69E-46
MS.gene055513	MS.gene38256	0.818671	1.74E-52	-1.69E-46
MS.gene055580	MS.gene38256	-0.867856	1.00E-65	-1.69E-46
MS.gene055808	MS.gene38256	0.824032	1.01E-53	-1.69E-46
MS.gene055979	MS.gene38256	0.830625	2.64E-55	-1.69E-46
MS.gene056043	MS.gene38256	-0.872124	4.05E-67	-1.69E-46
MS.gene056075	MS.gene38256	0.839209	1.82E-57	-1.69E-46
MS.gene056091	MS.gene38256	0.812167	4.86E-51	-1.69E-46
MS.gene056215	MS.gene38256	0.863586	2.24E-64	-1.69E-46
MS.gene056432	MS.gene38256	0.806083	9.77E-50	-1.69E-46
MS.gene056580	MS.gene38256	0.816287	5.98E-52	-1.69E-46
MS.gene056631	MS.gene38256	0.838103	3.51E-57	-1.69E-46
MS.gene056986	MS.gene38256	0.806585	7.66E-50	-1.69E-46
MS.gene057057	MS.gene38256	0.803032	4.23E-49	-1.69E-46
MS.gene057094	MS.gene38256	0.859179	4.95E-63	-1.69E-46
MS.gene057102	MS.gene38256	0.804203	2.42E-49	-1.69E-46
MS.gene057705	MS.gene38256	0.861776	8.09E-64	-1.69E-46
MS.gene058084	MS.gene38256	0.813984	1.94E-51	-1.69E-46
MS.gene058906	MS.gene38256	0.831972	1.23E-55	-1.69E-46
MS.gene059146	MS.gene38256	0.824534	7.66E-54	-1.69E-46
MS.gene059227	MS.gene38256	0.816783	4.63E-52	-1.69E-46
MS.gene05954	MS.gene38256	0.811973	5.35E-51	-1.69E-46
MS.gene05978	MS.gene38256	0.807123	5.89E-50	-1.69E-46
MS.gene059842	MS.gene38256	0.806469	8.10E-50	-1.69E-46
MS.gene06001	MS.gene38256	0.874701	5.50E-68	-1.69E-46
MS.gene06011	MS.gene38256	0.828393	9.22E-55	-1.69E-46
MS.gene060194	MS.gene38256	0.817893	2.60E-52	-1.69E-46
MS.gene060429	MS.gene38256	0.884327	2.11E-71	-1.69E-46
MS.gene060587	MS.gene38256	0.842579	2.37E-58	-1.69E-46
MS.gene060763	MS.gene38256	0.815705	8.06E-52	-1.69E-46
MS.gene060917	MS.gene38256	0.824234	9.01E-54	-1.69E-46
MS.gene061006	MS.gene38256	0.822272	2.59E-53	-1.69E-46
MS.gene061100	MS.gene38256	0.825883	3.68E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38256.t1	MTR_5g096540	100.000	117	0	0	1	117	30	146	4.34e-81	234
MS.gene38256.t1	MTR_5g015570	91.453	117	10	0	1	117	30	146	4.57e-75	219
MS.gene38256.t1	MTR_2g012450	90.598	117	11	0	1	117	30	146	1.72e-74	218
MS.gene38256.t1	MTR_4g126220	86.275	51	7	0	1	51	120	170	2.15e-26	97.1
MS.gene38256.t1	MTR_8g073140	54.651	86	12	1	1	86	1	59	6.27e-22	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene38256.t1	AT3G49910	87.179	117	15	0	1	117	30	146	1.27e-74	218
MS.gene38256.t1	AT5G67510	84.615	117	18	0	1	117	30	146	3.41e-71	209

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGGAACGGACGTTGTATT+TGG	0.245673	5.3:+77964874	None:intergenic
ATTACTCGTGAGAAAGTTAA+TGG	0.246639	5.3:-77964743	MS.gene38256:CDS
AAGGAGAAGGGTACTAAGTT+TGG	0.353717	5.3:-77964605	MS.gene38256:CDS
GATGAAGTTCAAGTTGTTAG+AGG	0.371888	5.3:-77964836	MS.gene38256:CDS
TGTTGTTACTAAGCTGAGAT+TGG	0.397665	5.3:-77964684	MS.gene38256:CDS
AGGACGTGCTGCTGCGGATA+AGG	0.407566	5.3:-77964624	MS.gene38256:CDS
ACTTCATCGTCTTTGCGAAC+TGG	0.423245	5.3:+77964851	None:intergenic
ATCGTCTTTGCGAACTGGTA+TGG	0.437342	5.3:+77964856	None:intergenic
TACTAAGCTGAGATTGGATA+AGG	0.440322	5.3:-77964678	MS.gene38256:CDS
CAGCTTAGTAACAACAACTT+TGG	0.443241	5.3:+77964691	None:intergenic
AATGGTTCTACTGTTAATGT+TGG	0.493059	5.3:-77964725	MS.gene38256:CDS
GTTGTATTTGGATCGGAGAT+CGG	0.508778	5.3:+77964886	None:intergenic
TTAGTAACAACAACTTTGGA+AGG	0.516252	5.3:+77964695	None:intergenic
GAAGTCGTTGCTTGAGAGGA+AGG	0.531193	5.3:-77964651	MS.gene38256:CDS
GTTGTTAGAGGAACATTCAA+AGG	0.548391	5.3:-77964824	MS.gene38256:CDS
ATAGGAAGTCGTTGCTTGAG+AGG	0.550235	5.3:-77964655	MS.gene38256:CDS
AGCTGAGATTGGATAAGGAT+AGG	0.551181	5.3:-77964673	MS.gene38256:CDS
TGCTGCTGCGGATAAGGAGA+AGG	0.551652	5.3:-77964618	MS.gene38256:CDS
GGCGAAAGGACGTGCTGCTG+CGG	0.551879	5.3:-77964630	MS.gene38256:CDS
AACGGACGTTGTATTTGGAT+CGG	0.584986	5.3:+77964879	None:intergenic
GGAACATTCAAAGGTCGTGA+AGG	0.623758	5.3:-77964815	MS.gene38256:CDS
TTGCTTGAGAGGAAGGCGAA+AGG	0.623866	5.3:-77964644	MS.gene38256:CDS
GTATTTGGATCGGAGATCGG+TGG	0.651925	5.3:+77964889	None:intergenic
GCTGCTGCGGATAAGGAGAA+GGG	0.652585	5.3:-77964617	MS.gene38256:CDS
GATCGGAGATCGGTGGACAG+TGG	0.656057	5.3:+77964896	None:intergenic
CTTTGCGAACTGGTATGGAA+CGG	0.671113	5.3:+77964861	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAGTTTATCGCAAAAAAT+GGG	-	chr5.3:77964696-77964715	MS.gene38256:CDS	25.0%
!!	TTCAAGTTTATCGCAAAAAA+TGG	-	chr5.3:77964695-77964714	MS.gene38256:CDS	25.0%
	ATTACTCGTGAGAAAGTTAA+TGG	-	chr5.3:77964736-77964755	MS.gene38256:CDS	30.0%
	TTAGTAACAACAACTTTGGA+AGG	+	chr5.3:77964787-77964806	None:intergenic	30.0%
!	AATGGTTCTACTGTTAATGT+TGG	-	chr5.3:77964754-77964773	MS.gene38256:CDS	30.0%
	CAGCTTAGTAACAACAACTT+TGG	+	chr5.3:77964791-77964810	None:intergenic	35.0%
	GTTGTTAGAGGAACATTCAA+AGG	-	chr5.3:77964655-77964674	MS.gene38256:CDS	35.0%
	TACTAAGCTGAGATTGGATA+AGG	-	chr5.3:77964801-77964820	MS.gene38256:CDS	35.0%
	TGTTGTTACTAAGCTGAGAT+TGG	-	chr5.3:77964795-77964814	MS.gene38256:CDS	35.0%
!!	GATGAAGTTCAAGTTGTTAG+AGG	-	chr5.3:77964643-77964662	MS.gene38256:CDS	35.0%
	AACGGACGTTGTATTTGGAT+CGG	+	chr5.3:77964603-77964622	None:intergenic	40.0%
	AGCTGAGATTGGATAAGGAT+AGG	-	chr5.3:77964806-77964825	MS.gene38256:CDS	40.0%
	GTTGTATTTGGATCGGAGAT+CGG	+	chr5.3:77964596-77964615	None:intergenic	40.0%
	TATGGAACGGACGTTGTATT+TGG	+	chr5.3:77964608-77964627	None:intergenic	40.0%
!!	AAGGAGAAGGGTACTAAGTT+TGG	-	chr5.3:77964874-77964893	MS.gene38256:CDS	40.0%
	ACTTCATCGTCTTTGCGAAC+TGG	+	chr5.3:77964631-77964650	None:intergenic	45.0%
	ATAGGAAGTCGTTGCTTGAG+AGG	-	chr5.3:77964824-77964843	MS.gene38256:CDS	45.0%
	ATCGTCTTTGCGAACTGGTA+TGG	+	chr5.3:77964626-77964645	None:intergenic	45.0%
	CTTTGCGAACTGGTATGGAA+CGG	+	chr5.3:77964621-77964640	None:intergenic	45.0%
	GGAACATTCAAAGGTCGTGA+AGG	-	chr5.3:77964664-77964683	MS.gene38256:CDS	45.0%
	GAAGTCGTTGCTTGAGAGGA+AGG	-	chr5.3:77964828-77964847	MS.gene38256:CDS	50.0%
	GTATTTGGATCGGAGATCGG+TGG	+	chr5.3:77964593-77964612	None:intergenic	50.0%
	TTGCTTGAGAGGAAGGCGAA+AGG	-	chr5.3:77964835-77964854	MS.gene38256:CDS	50.0%
	GCTGCTGCGGATAAGGAGAA+GGG	-	chr5.3:77964862-77964881	MS.gene38256:CDS	55.0%
	TGCTGCTGCGGATAAGGAGA+AGG	-	chr5.3:77964861-77964880	MS.gene38256:CDS	55.0%
	GATCGGAGATCGGTGGACAG+TGG	+	chr5.3:77964586-77964605	None:intergenic	60.0%
!	AGGACGTGCTGCTGCGGATA+AGG	-	chr5.3:77964855-77964874	MS.gene38256:CDS	60.0%
!	GGCGAAAGGACGTGCTGCTG+CGG	-	chr5.3:77964849-77964868	MS.gene38256:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	77964574	77964927	77964574	ID=MS.gene38256
chr5.3	mRNA	77964574	77964927	77964574	ID=MS.gene38256.t1;Parent=MS.gene38256
chr5.3	exon	77964574	77964927	77964574	ID=MS.gene38256.t1.exon1;Parent=MS.gene38256.t1
chr5.3	CDS	77964574	77964927	77964574	ID=cds.MS.gene38256.t1;Parent=MS.gene38256.t1

Gene Sequence	>MS.gene38256 ATGAGCGCACCACTGTCCACCGATCTCCGATCCAAATACAACGTCCGTTCCATACCAGTTCGCAAAGACGATGAAGTTCAAGTTGTTAGAGGAACATTCAAAGGTCGTGAAGGTAAAATCGTTCAAGTTTATCGCAAAAAATGGGTTATTCATATTGAACGTATTACTCGTGAGAAAGTTAATGGTTCTACTGTTAATGTTGGCGTTGATCCTTCCAAAGTTGTTGTTACTAAGCTGAGATTGGATAAGGATAGGAAGTCGTTGCTTGAGAGGAAGGCGAAAGGACGTGCTGCTGCGGATAAGGAGAAGGGTACTAAGTTTGGTGCTGAAGATATTATGCAGAATGTTGATTGA
Protein sequence	>MS.gene38256.t1 MSAPLSTDLRSKYNVRSIPVRKDDEVQVVRGTFKGREGKIVQVYRKKWVIHIERITREKVNGSTVNVGVDPSKVVVTKLRLDKDRKSLLERKAKGRAAADKEKGTKFGAEDIMQNVD