AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40513


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40513.t1 MTR_5g096490 96.923 130 4 0 34 163 4 133 3.05e-88 254
MS.gene40513.t1 MTR_5g007240 86.755 151 18 2 14 163 17 166 6.93e-81 236
MS.gene40513.t1 MTR_5g096490 86.755 151 18 2 14 163 17 166 6.93e-81 236
MS.gene40513.t1 MTR_4g051390 42.975 121 67 1 44 162 1 121 4.38e-31 108
MS.gene40513.t1 MTR_1779s0020 42.975 121 67 1 44 162 1 121 6.70e-30 105
MS.gene40513.t1 MTR_2g087550 41.667 120 68 1 45 162 2 121 4.40e-28 103
MS.gene40513.t1 MTR_0002s0780 41.739 115 64 1 51 162 135 249 4.42e-24 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40513.t1 AT5G46160 78.030 132 22 1 39 163 41 172 6.31e-67 201
MS.gene40513.t1 AT5G46160 78.030 132 22 1 39 163 42 173 7.20e-67 201
MS.gene40513.t1 AT1G17560 60.131 153 32 3 39 162 43 195 4.06e-53 167
MS.gene40513.t1 AT1G17560 60.131 153 32 3 39 162 20 172 4.20e-53 166
MS.gene40513.t1 ATCG00780 44.628 121 65 1 44 162 1 121 1.40e-32 112

Find 41 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAGGTTGTGGACAATTC+TGG 0.240087 5.3:-2103631 MS.gene40513:CDS
GGACAATTCTGGAGCTAAAA+AGG 0.321429 5.3:-2103620 MS.gene40513:CDS
GACAAACAAGGGCAGCCTAT+TGG 0.362318 5.3:-2103397 MS.gene40513:CDS
ACAAACAAGGGCAGCCTATT+GGG 0.366691 5.3:-2103396 MS.gene40513:CDS
GTCAAGATCCTTACCTTAGC+TGG 0.374088 5.3:-2103325 MS.gene40513:CDS
GTGAAGGAAGCACATCCTAA+TGG 0.397241 5.3:-2103532 MS.gene40513:CDS
TGCCTCAACTCATGAGGAAC+TGG 0.420523 5.3:+2103358 None:intergenic
CCGAAAACTCTGGTCCCAAT+AGG 0.444490 5.3:+2103382 None:intergenic
CGCGCGTTGGCAGGGAAGAA+AGG 0.457182 5.3:-2103586 MS.gene40513:CDS
GGGAAGAAAGGAGCAAGATT+AGG 0.462937 5.3:-2103574 MS.gene40513:CDS
ATGTGCATACGCGCGTTGGC+AGG 0.463329 5.3:-2103595 MS.gene40513:CDS
CGTGTTTGACTTGTAAATAG+AGG 0.479650 5.3:+2104125 None:intergenic
TCCTAATGGAAAAGTGAAGA+AGG 0.493277 5.3:-2103518 MS.gene40513:CDS
CTGCCATCACACCTACCCTT+TGG 0.494271 5.3:+2103457 None:intergenic
TAGAGGGAGGGAGTGATTAG+TGG 0.520080 5.3:+2104142 None:intergenic
AACAGTCCTCATCTGTATGA+AGG 0.520727 5.3:+2103654 None:intergenic
ATGCCAAAGGGTAGGTGTGA+TGG 0.533908 5.3:-2103460 MS.gene40513:CDS
CAATATGCCCAGCTAAGGTA+AGG 0.539065 5.3:+2103317 None:intergenic
CATTGCTGCACGAACAACTA+CGG 0.550383 5.3:+2103480 None:intergenic
AGGAACTGGTCCGAAAACTC+TGG 0.550755 5.3:+2103372 None:intergenic
TCAAGATCCTTACCTTAGCT+GGG 0.562147 5.3:-2103324 MS.gene40513:CDS
CTATGCAATATGCCCAGCTA+AGG 0.565511 5.3:+2103312 None:intergenic
TGTTCGTGCAGCAATGCCAA+AGG 0.568662 5.3:-2103473 MS.gene40513:CDS
GTTAAAATGTCAACTTCCCC+AGG 0.577318 5.3:-2104410 MS.gene40513:intron
TGTGCATACGCGCGTTGGCA+GGG 0.580036 5.3:-2103594 MS.gene40513:CDS
GTGTTTGACTTGTAAATAGA+GGG 0.580442 5.3:+2104126 None:intergenic
GACCAGTTCCTCATGAGTTG+AGG 0.580661 5.3:-2103360 MS.gene40513:CDS
ACAGATGAGGACTGTTCTGA+AGG 0.580846 5.3:-2103647 MS.gene40513:CDS
TTGACTTGTAAATAGAGGGA+GGG 0.582627 5.3:+2104130 None:intergenic
GAGGACTGTTCTGAAGGTTG+TGG 0.589490 5.3:-2103641 MS.gene40513:CDS
GGTGATGTGCATACGCGCGT+TGG 0.592616 5.3:-2103599 MS.gene40513:CDS
GTTCGTGCAGCAATGCCAAA+GGG 0.599925 5.3:-2103472 MS.gene40513:CDS
CCTAATGGAAAAGTGAAGAA+GGG 0.626002 5.3:-2103517 MS.gene40513:CDS
TTCTTCTGCCTCAACTCATG+AGG 0.632900 5.3:+2103352 None:intergenic
TTTGACTTGTAAATAGAGGG+AGG 0.634315 5.3:+2104129 None:intergenic
GTGCAGCAATGCCAAAGGGT+AGG 0.641762 5.3:-2103468 MS.gene40513:CDS
GTTGTGCTTGTTGACAAACA+AGG 0.656177 5.3:-2103409 MS.gene40513:CDS
TATGATACAAGCATCAGTGA+AGG 0.662607 5.3:-2103548 MS.gene40513:CDS
AACGAACCTTCATACAGATG+AGG 0.663831 5.3:-2103660 MS.gene40513:CDS
TTGTGCTTGTTGACAAACAA+GGG 0.669343 5.3:-2103408 MS.gene40513:CDS
GGGTAGGTGTGATGGCAGTG+AGG 0.701393 5.3:-2103452 MS.gene40513:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAATATCTATTAAG+TGG + chr5.3:2103678-2103697 None:intergenic 10.0%
!! AGCATAAGAAAATAATGTTA+AGG + chr5.3:2103883-2103902 None:intergenic 20.0%
!! TAATATATGAAATTTCAGAG+TGG + chr5.3:2103541-2103560 None:intergenic 20.0%
!!! CAAGAATTTTTATGTTGATA+AGG - chr5.3:2103641-2103660 MS.gene40513:CDS 20.0%
!!! GTTTTAGTAAACTAACAATT+TGG + chr5.3:2103417-2103436 None:intergenic 20.0%
! GTAATAATGAAACTACAACA+TGG + chr5.3:2103439-2103458 None:intergenic 25.0%
! GTTGTAGTTTCATTATTACA+TGG - chr5.3:2103440-2103459 MS.gene40513:CDS 25.0%
!!! AAACATTGATTCTATCCTTT+TGG - chr5.3:2103382-2103401 MS.gene40513:CDS 25.0%
AAAACAGTTATTCTCTTACC+TGG + chr5.3:2103350-2103369 None:intergenic 30.0%
AAACAGTTATTCTCTTACCT+GGG + chr5.3:2103349-2103368 None:intergenic 30.0%
AAGATTACCAATACTGTGTT+TGG + chr5.3:2103908-2103927 None:intergenic 30.0%
TGTTGAAAAACCAAACTAGT+GGG - chr5.3:2103721-2103740 MS.gene40513:intron 30.0%
! TATTTTTCTCAGGTACATGT+AGG - chr5.3:2103820-2103839 MS.gene40513:intron 30.0%
!! GTGTTTGACTTGTAAATAGA+GGG + chr5.3:2103616-2103635 None:intergenic 30.0%
!!! ACTGTTGTTTTCTACAATCT+AGG + chr5.3:2103934-2103953 None:intergenic 30.0%
!!! ATGCAACAGTTATTTTTCTC+AGG - chr5.3:2103810-2103829 MS.gene40513:intron 30.0%
!!! CATTTTAACTTCCGATGAAA+AGG + chr5.3:2103318-2103337 None:intergenic 30.0%
AACAGTTATTCTCTTACCTG+GGG + chr5.3:2103348-2103367 None:intergenic 35.0%
CCTAATGGAAAAGTGAAGAA+GGG - chr5.3:2104222-2104241 MS.gene40513:intron 35.0%
GTGTTGAAAAACCAAACTAG+TGG - chr5.3:2103720-2103739 MS.gene40513:intron 35.0%
TATGATACAAGCATCAGTGA+AGG - chr5.3:2104191-2104210 MS.gene40513:intron 35.0%
TCCTAATGGAAAAGTGAAGA+AGG - chr5.3:2104221-2104240 MS.gene40513:intron 35.0%
TGTGTGTAAGAAACTAAGCA+TGG + chr5.3:2104009-2104028 None:intergenic 35.0%
! AGACATTGTTTTCTTCAGGA+AGG + chr5.3:2104043-2104062 None:intergenic 35.0%
! AGGAAGACATTGTTTTCTTC+AGG + chr5.3:2104047-2104066 None:intergenic 35.0%
! TTGCCAATGCTCATGTTTTA+GGG + chr5.3:2103476-2103495 None:intergenic 35.0%
! TTGTGCTTGTTGACAAACAA+GGG - chr5.3:2104331-2104350 MS.gene40513:intron 35.0%
! TTTGCCAATGCTCATGTTTT+AGG + chr5.3:2103477-2103496 None:intergenic 35.0%
!! CGTGTTTGACTTGTAAATAG+AGG + chr5.3:2103617-2103636 None:intergenic 35.0%
!! TTGACTTGTAAATAGAGGGA+GGG + chr5.3:2103612-2103631 None:intergenic 35.0%
!! TTTGACTTGTAAATAGAGGG+AGG + chr5.3:2103613-2103632 None:intergenic 35.0%
!!! CATTGATTCTATCCTTTTGG+TGG - chr5.3:2103385-2103404 MS.gene40513:CDS 35.0%
AACAGTCCTCATCTGTATGA+AGG + chr5.3:2104088-2104107 None:intergenic 40.0%
AACGAACCTTCATACAGATG+AGG - chr5.3:2104079-2104098 MS.gene40513:intron 40.0%
ACATGTATCTTTGCACTGCA+TGG + chr5.3:2103964-2103983 None:intergenic 40.0%
AGAGTGGTTGCAAAATGTAG+TGG + chr5.3:2103525-2103544 None:intergenic 40.0%
ATGCTTGCCAAACACAGTAT+TGG - chr5.3:2103898-2103917 MS.gene40513:intron 40.0%
ATTTGGACATCACCACCAAA+AGG + chr5.3:2103400-2103419 None:intergenic 40.0%
GTTAAAATGTCAACTTCCCC+AGG - chr5.3:2103329-2103348 MS.gene40513:CDS 40.0%
TACATGTAGGAATCAGTTGC+AGG - chr5.3:2103833-2103852 MS.gene40513:intron 40.0%
TCAAGATCCTTACCTTAGCT+GGG - chr5.3:2104415-2104434 MS.gene40513:CDS 40.0%
TTAATCCACCGAACAGAGAA+CGG + chr5.3:2103747-2103766 None:intergenic 40.0%
! GTTGTGCTTGTTGACAAACA+AGG - chr5.3:2104330-2104349 MS.gene40513:intron 40.0%
!! CCCTTCTTCACTTTTCCATT+AGG + chr5.3:2104225-2104244 None:intergenic 40.0%
!! GGACAATTCTGGAGCTAAAA+AGG - chr5.3:2104119-2104138 MS.gene40513:CDS 40.0%
ACAAACAAGGGCAGCCTATT+GGG - chr5.3:2104343-2104362 MS.gene40513:intron 45.0%
ACAGATGAGGACTGTTCTGA+AGG - chr5.3:2104092-2104111 MS.gene40513:intron 45.0%
CAATATGCCCAGCTAAGGTA+AGG + chr5.3:2104425-2104444 None:intergenic 45.0%
CATTGCTGCACGAACAACTA+CGG + chr5.3:2104262-2104281 None:intergenic 45.0%
CTATGCAATATGCCCAGCTA+AGG + chr5.3:2104430-2104449 None:intergenic 45.0%
CTGAAGGTTGTGGACAATTC+TGG - chr5.3:2104108-2104127 MS.gene40513:CDS 45.0%
GCTCCCTAAAACATGAGCAT+TGG - chr5.3:2103470-2103489 MS.gene40513:CDS 45.0%
GGGAAGAAAGGAGCAAGATT+AGG - chr5.3:2104165-2104184 MS.gene40513:intron 45.0%
GGTTCGTTGTTGCTGCAAAA+AGG + chr5.3:2104067-2104086 None:intergenic 45.0%
GTCAAGATCCTTACCTTAGC+TGG - chr5.3:2104414-2104433 MS.gene40513:CDS 45.0%
GTGACGTGGTAAATAGACCA+GGG + chr5.3:2103780-2103799 None:intergenic 45.0%
TGACGTGGTAAATAGACCAG+GGG + chr5.3:2103779-2103798 None:intergenic 45.0%
TGTGACGTGGTAAATAGACC+AGG + chr5.3:2103781-2103800 None:intergenic 45.0%
TTAGTGGCACAAGTGCAAAC+TGG + chr5.3:2103584-2103603 None:intergenic 45.0%
TTCTTCTGCCTCAACTCATG+AGG + chr5.3:2104390-2104409 None:intergenic 45.0%
! CCTATTGGGACCAGAGTTTT+CGG - chr5.3:2104357-2104376 MS.gene40513:intron 45.0%
! GTGAAGGAAGCACATCCTAA+TGG - chr5.3:2104207-2104226 MS.gene40513:intron 45.0%
AGGAACTGGTCCGAAAACTC+TGG + chr5.3:2104370-2104389 None:intergenic 50.0%
ATGCCAAAGGGTAGGTGTGA+TGG - chr5.3:2104279-2104298 MS.gene40513:intron 50.0%
CCGAAAACTCTGGTCCCAAT+AGG + chr5.3:2104360-2104379 None:intergenic 50.0%
GACAAACAAGGGCAGCCTAT+TGG - chr5.3:2104342-2104361 MS.gene40513:intron 50.0%
GACCAGTTCCTCATGAGTTG+AGG - chr5.3:2104379-2104398 MS.gene40513:intron 50.0%
GCATGTCATCTCATGTGACG+TGG + chr5.3:2103794-2103813 None:intergenic 50.0%
GGATTAAGCAACAGTTCCCC+TGG - chr5.3:2103760-2103779 MS.gene40513:intron 50.0%
GTTCGTGCAGCAATGCCAAA+GGG - chr5.3:2104267-2104286 MS.gene40513:intron 50.0%
TAGAGGGAGGGAGTGATTAG+TGG + chr5.3:2103600-2103619 None:intergenic 50.0%
TGCCTCAACTCATGAGGAAC+TGG + chr5.3:2104384-2104403 None:intergenic 50.0%
TGTTCGTGCAGCAATGCCAA+AGG - chr5.3:2104266-2104285 MS.gene40513:intron 50.0%
!! GAGGACTGTTCTGAAGGTTG+TGG - chr5.3:2104098-2104117 MS.gene40513:intron 50.0%
CAGAGAACGGCCCACTAGTT+TGG + chr5.3:2103734-2103753 None:intergenic 55.0%
CTGCCATCACACCTACCCTT+TGG + chr5.3:2104285-2104304 None:intergenic 55.0%
GTGCAGCAATGCCAAAGGGT+AGG - chr5.3:2104271-2104290 MS.gene40513:intron 55.0%
! CTAGTGGGCCGTTCTCTGTT+CGG - chr5.3:2103736-2103755 MS.gene40513:intron 55.0%
GGGTAGGTGTGATGGCAGTG+AGG - chr5.3:2104287-2104306 MS.gene40513:intron 60.0%
GGTGATGTGCATACGCGCGT+TGG - chr5.3:2104140-2104159 MS.gene40513:CDS 60.0%
! ATGTGCATACGCGCGTTGGC+AGG - chr5.3:2104144-2104163 MS.gene40513:CDS 60.0%
! TGTGCATACGCGCGTTGGCA+GGG - chr5.3:2104145-2104164 MS.gene40513:CDS 60.0%
GTGGGCCGTTCTCTGTTCGG+TGG - chr5.3:2103739-2103758 MS.gene40513:intron 65.0%
! CGCGCGTTGGCAGGGAAGAA+AGG - chr5.3:2104153-2104172 MS.gene40513:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 2103312 2104449 2103312 ID=MS.gene40513
chr5.3 mRNA 2103312 2104449 2103312 ID=MS.gene40513.t1;Parent=MS.gene40513
chr5.3 exon 2104411 2104449 2104411 ID=MS.gene40513.t1.exon1;Parent=MS.gene40513.t1
chr5.3 CDS 2104411 2104449 2104411 ID=cds.MS.gene40513.t1;Parent=MS.gene40513.t1
chr5.3 exon 2104099 2104176 2104099 ID=MS.gene40513.t1.exon2;Parent=MS.gene40513.t1
chr5.3 CDS 2104099 2104176 2104099 ID=cds.MS.gene40513.t1;Parent=MS.gene40513.t1
chr5.3 exon 2103312 2103686 2103312 ID=MS.gene40513.t1.exon3;Parent=MS.gene40513.t1
chr5.3 CDS 2103312 2103686 2103312 ID=cds.MS.gene40513.t1;Parent=MS.gene40513.t1
Gene Sequence

>MS.gene40513

ATGCCTTTTCATCGGAAGTTAAAATGTCAACTTCCCCAGGTAAGAGAATAACTGTTTTCTATGTCTTGCAAAACATTGATTCTATCCTTTTGGTGGTGATGTCCAAATTGTTAGTTTACTAAAACCATGTTGTAGTTTCATTATTACATGGTCATTTTGCTCCCTAAAACATGAGCATTGGCAAACTGTTGCTTTTGCAGTAAATTGAAACCACTACATTTTGCAACCACTCTGAAATTTCATATATTAAGCTGCATGACTCTATCTTGCCAGTTTGCACTTGTGCCACTAATCACTCCCTCCCTCTATTTACAAGTCAAACACGAGCTCAAGAATTTTTATGTTGATAAGGTGATACTTTATCCACTTAATAGATATTTTTATTAACTTATTAGTTTTTTGTTTCATGTGTTGAAAAACCAAACTAGTGGGCCGTTCTCTGTTCGGTGGATTAAGCAACAGTTCCCCTGGTCTATTTACCACGTCACATGAGATGACATGCAACAGTTATTTTTCTCAGGTACATGTAGGAATCAGTTGCAGGATCATGTTTCAATTATTATAATTTCCTTAACATTATTTTCTTATGCTTGCCAAACACAGTATTGGTAATCTTAAACCTAGATTGTAGAAAACAACAGTAATTTTTCCATGCAGTGCAAAGATACATGTGTTCTTAAAATGAGTCACAAATCCATGCTTAGTTTCTTACACACATTGTTATGATTCCTTCCTGAAGAAAACAATGTCTTCCTTTTTGCAGCAACAACGAACCTTCATACAGATGAGGACTGTTCTGAAGGTTGTGGACAATTCTGGAGCTAAAAAGGTGATGTGCATACGCGCGTTGGCAGGGAAGAAAGGAGCAAGATTAGGTGATATGATACAAGCATCAGTGAAGGAAGCACATCCTAATGGAAAAGTGAAGAAGGGAGCTGTTGTATTTGCCGTAGTTGTTCGTGCAGCAATGCCAAAGGGTAGGTGTGATGGCAGTGAGGTCAAGTTTGACGACAATGCTGTTGTGCTTGTTGACAAACAAGGGCAGCCTATTGGGACCAGAGTTTTCGGACCAGTTCCTCATGAGTTGAGGCAGAAGAAGCATGTCAAGATCCTTACCTTAGCTGGGCATATTGCATAG

Protein sequence

>MS.gene40513.t1

MPFHRKLKCQLPQFALVPLITPSLYLQVKHELKNFYVDKQQRTFIQMRTVLKVVDNSGAKKVMCIRALAGKKGARLGDMIQASVKEAHPNGKVKKGAVVFAVVVRAAMPKGRCDGSEVKFDDNAVVLVDKQGQPIGTRVFGPVPHELRQKKHVKILTLAGHIA