AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44654


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44654.t1 MTR_7g015010 97.436 351 9 0 1 351 1 351 0.0 698
MS.gene44654.t1 MTR_6g015350 42.973 370 154 15 1 351 2 333 7.44e-63 204
MS.gene44654.t1 MTR_4g109660 62.185 119 42 2 1 118 1 117 2.79e-45 157
MS.gene44654.t1 MTR_3g026050 44.783 230 101 9 1 209 1 225 1.31e-44 157
MS.gene44654.t1 MTR_8g463380 60.526 76 30 0 51 126 48 123 2.59e-24 103
MS.gene44654.t1 MTR_4g079580 58.442 77 32 0 51 127 16 92 6.67e-24 100
MS.gene44654.t1 MTR_2g078200 61.644 73 28 0 51 123 14 86 9.70e-24 99.8
MS.gene44654.t1 MTR_2g090960 60.811 74 29 0 51 124 18 91 2.95e-23 99.0
MS.gene44654.t1 MTR_4g111935 29.856 278 155 11 18 264 90 358 1.10e-15 77.8
MS.gene44654.t1 MTR_6g017055 39.623 106 58 3 58 158 127 231 9.28e-14 72.4
MS.gene44654.t1 MTR_7g018500 50.877 57 28 0 59 115 122 178 4.65e-13 70.1
MS.gene44654.t1 MTR_1g103380 48.333 60 31 0 56 115 200 259 1.07e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44654.t1 AT3G02150 75.000 100 25 0 30 129 47 146 1.54e-45 157
MS.gene44654.t1 AT3G02150 75.000 100 25 0 30 129 47 146 3.93e-45 158
MS.gene44654.t1 AT5G08070 71.717 99 24 2 25 123 4 98 2.85e-42 147
MS.gene44654.t1 AT5G08070 71.717 99 24 2 25 123 4 98 2.85e-42 147
MS.gene44654.t1 AT5G60970 83.750 80 12 1 43 122 49 127 5.71e-42 150
MS.gene44654.t1 AT1G30210 75.000 72 18 0 47 118 40 111 9.11e-30 116
MS.gene44654.t1 AT1G30210 75.000 72 18 0 47 118 40 111 9.11e-30 116
MS.gene44654.t1 AT4G18390 35.000 260 112 8 47 298 32 242 2.85e-27 110
MS.gene44654.t1 AT4G18390 35.000 260 112 8 47 298 32 242 2.85e-27 110
MS.gene44654.t1 AT1G53230 61.842 76 29 0 51 126 43 118 2.02e-25 105
MS.gene44654.t1 AT1G53230 61.842 76 29 0 51 126 43 118 2.02e-25 105
MS.gene44654.t1 AT3G15030 60.811 74 29 0 51 124 39 112 1.04e-23 101
MS.gene44654.t1 AT3G15030 60.811 74 29 0 51 124 39 112 1.04e-23 101
MS.gene44654.t1 AT3G15030 60.811 74 29 0 51 124 39 112 1.04e-23 101
MS.gene44654.t1 AT3G15030 60.811 74 29 0 51 124 39 112 1.04e-23 101
MS.gene44654.t1 AT2G31070 64.615 65 23 0 51 115 23 87 5.13e-21 93.2
MS.gene44654.t1 AT1G68800 47.761 67 35 0 49 115 104 170 5.29e-15 75.5
MS.gene44654.t1 AT1G68800 47.761 67 35 0 49 115 104 170 6.04e-15 75.5
MS.gene44654.t1 AT1G67260 42.105 76 44 0 46 121 76 151 6.22e-15 75.5
MS.gene44654.t1 AT1G67260 42.105 76 44 0 46 121 76 151 6.22e-15 75.5
MS.gene44654.t1 AT1G67260 42.105 76 44 0 46 121 76 151 6.75e-15 75.5
MS.gene44654.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.22e-13 72.0
MS.gene44654.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.36e-13 72.0
MS.gene44654.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.36e-13 72.0
MS.gene44654.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.55e-13 71.6

Find 61 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAATCACCCTTCATTTAT+TGG 0.099357 7.3:-83202945 MS.gene44654:CDS
TGTAACATATTGTTATTATT+TGG 0.154767 7.3:+83202972 None:intergenic
CTTCAAGTCAAAATTCTTCT+TGG 0.156785 7.3:-83203127 MS.gene44654:CDS
AGTGAACTCTTGTTCTTTAT+TGG 0.160335 7.3:+83202572 None:intergenic
AATTCTCTTGAAACTTCTTT+AGG 0.204373 7.3:+83203095 None:intergenic
GTTCCTACAGGTGGAAAATT+AGG 0.209351 7.3:+83203242 None:intergenic
CTTGATACTCTGACAATTCT+TGG 0.239929 7.3:+83203461 None:intergenic
GTACTATTCATCAAGTAATT+TGG 0.245121 7.3:+83203059 None:intergenic
TGCTGAAAATTCTTCTCAAT+TGG 0.271973 7.3:-83202865 MS.gene44654:CDS
ATTCTCTTGAAACTTCTTTA+GGG 0.288069 7.3:+83203096 None:intergenic
CCAATAAATGAAGGGTGATT+TGG 0.289695 7.3:+83202945 None:intergenic
ATTCTTGGATCTTTCAATCT+TGG 0.296306 7.3:+83203476 None:intergenic
GTCAGAGTATCAAGAGCTTT+TGG 0.318336 7.3:-83203452 MS.gene44654:CDS
TAAAGAAGTTTCAAGAGAAT+TGG 0.320601 7.3:-83203093 MS.gene44654:CDS
AATTCTTCTCAAATGATGTT+AGG 0.334825 7.3:-83202789 MS.gene44654:CDS
TCAATAGGAACAATATTCAT+TGG 0.339202 7.3:-83203160 MS.gene44654:CDS
TATGATCTTCAAGATAGGTT+AGG 0.344793 7.3:-83203341 MS.gene44654:CDS
GATTGAGAGATAGAAGAATA+AGG 0.365377 7.3:-83203391 MS.gene44654:CDS
CCAATGTTGTCTTTATCAAC+TGG 0.374206 7.3:-83202813 MS.gene44654:CDS
TGATGAACCTCTTGTTTCAA+TGG 0.375029 7.3:+83203569 None:intergenic
AACAATTGTTCAATTTCAAT+AGG 0.377158 7.3:-83203175 MS.gene44654:CDS
AACCTAGCAAAGTTGTTGAT+TGG 0.387978 7.3:-83203310 MS.gene44654:CDS
GCTGAAAATTCTTCTCAATT+GGG 0.390841 7.3:-83202864 MS.gene44654:CDS
CAACAATCCATCCTTCAGTT+TGG 0.407175 7.3:-83202628 MS.gene44654:CDS
GTAGGAACAAATTTCAACTT+TGG 0.419771 7.3:-83203227 MS.gene44654:CDS
AACTTTATGATCTTCAAGAT+AGG 0.421843 7.3:-83203346 MS.gene44654:CDS
ATGATCTTCAAGATAGGTTA+GGG 0.433727 7.3:-83203340 MS.gene44654:CDS
AGCAAAGTATGCACAATAAG+AGG 0.437085 7.3:-83203413 MS.gene44654:CDS
TTTAATAAACCAATAAATGA+AGG 0.455410 7.3:+83202936 None:intergenic
CAATAGGAACAATATTCATT+GGG 0.458388 7.3:-83203159 MS.gene44654:CDS
TTGAAATTTGTTCCTACAGG+TGG 0.465228 7.3:+83203233 None:intergenic
TTGTTGAGAGAATTAGTCAT+TGG 0.470696 7.3:+83202645 None:intergenic
AAGTTGAAATTTGTTCCTAC+AGG 0.474911 7.3:+83203230 None:intergenic
TTTGATGATAATGATGGTTG+TGG 0.483862 7.3:+83202697 None:intergenic
ACTTGGTACTAATTACCAAT+GGG 0.492610 7.3:-83202904 MS.gene44654:CDS
TTCTTCAAATGTTAATGCAA+TGG 0.492790 7.3:-83202748 MS.gene44654:CDS
TAGAAATTTGGCCAAACTGA+AGG 0.496121 7.3:+83202617 None:intergenic
TTGTTGAGTTCATTAGAAGA+TGG 0.499012 7.3:+83203200 None:intergenic
TCTTCTCAATTGGGAAACAA+TGG 0.501500 7.3:-83202855 MS.gene44654:CDS
TTATTAAATCCTATGCCACT+TGG 0.521662 7.3:-83202921 MS.gene44654:CDS
AAATGTTAATGCAATGGAGA+TGG 0.523566 7.3:-83202742 MS.gene44654:CDS
ACAGGTGGAAAATTAGGTAA+TGG 0.536982 7.3:+83203248 None:intergenic
CCAGTTGATAAAGACAACAT+TGG 0.545201 7.3:+83202813 None:intergenic
TCAACATTTGATGATAATGA+TGG 0.545561 7.3:+83202691 None:intergenic
AACCAATCAACAACTTTGCT+AGG 0.547433 7.3:+83203308 None:intergenic
TAATTAGTACCAAGTGGCAT+AGG 0.547722 7.3:+83202912 None:intergenic
GATGAAAATATTAACAAGCA+AGG 0.549563 7.3:-83203029 MS.gene44654:CDS
CACTTGGTACTAATTACCAA+TGG 0.556420 7.3:-83202905 MS.gene44654:CDS
CATTGGAAGTACCTCCCCAT+TGG 0.558728 7.3:+83202889 None:intergenic
AATTTGGCCAAACTGAAGGA+TGG 0.562622 7.3:+83202621 None:intergenic
TGGCCATTGTGATGAAGCTA+AGG 0.563573 7.3:+83203496 None:intergenic
AGGAACAATATTCATTGGGA+AGG 0.563907 7.3:-83203155 MS.gene44654:CDS
GGTGGAAAATTAGGTAATGG+TGG 0.564196 7.3:+83203251 None:intergenic
GTTGATAAAGACAACATTGG+TGG 0.570845 7.3:+83202816 None:intergenic
TCATAAAGTTGAATAGCTGT+TGG 0.592296 7.3:+83203359 None:intergenic
TTAATAAACCAATAAATGAA+GGG 0.612685 7.3:+83202937 None:intergenic
GAAGCTAAGGAGCTTGATGA+TGG 0.616225 7.3:+83203509 None:intergenic
GCTCCTTAGCTTCATCACAA+TGG 0.647279 7.3:-83203499 MS.gene44654:CDS
GGTACTAATTACCAATGGGG+AGG 0.679474 7.3:-83202900 MS.gene44654:CDS
CATTGGTAATTAGTACCAAG+TGG 0.701178 7.3:+83202906 None:intergenic
CTTGGTACTAATTACCAATG+GGG 0.737610 7.3:-83202903 MS.gene44654:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAAACCAATAAATGAA+GGG + chr7.3:83203225-83203244 None:intergenic 15.0%
!! TTTAATAAACCAATAAATGA+AGG + chr7.3:83203226-83203245 None:intergenic 15.0%
!!! TGTAACATATTGTTATTATT+TGG + chr7.3:83203190-83203209 None:intergenic 15.0%
!! AACAATTGTTCAATTTCAAT+AGG - chr7.3:83202984-83203003 MS.gene44654:CDS 20.0%
! AAACGATCATGAAAAAGAAA+AGG - chr7.3:83202619-83202638 MS.gene44654:CDS 25.0%
! AACTTTATGATCTTCAAGAT+AGG - chr7.3:83202813-83202832 MS.gene44654:CDS 25.0%
! AATTCTCTTGAAACTTCTTT+AGG + chr7.3:83203067-83203086 None:intergenic 25.0%
! AATTCTTCTCAAATGATGTT+AGG - chr7.3:83203370-83203389 MS.gene44654:CDS 25.0%
! ATTCTCTTGAAACTTCTTTA+GGG + chr7.3:83203066-83203085 None:intergenic 25.0%
! CAATAGGAACAATATTCATT+GGG - chr7.3:83203000-83203019 MS.gene44654:CDS 25.0%
! GATGAAAATATTAACAAGCA+AGG - chr7.3:83203130-83203149 MS.gene44654:CDS 25.0%
! GTACTATTCATCAAGTAATT+TGG + chr7.3:83203103-83203122 None:intergenic 25.0%
! TAAAGAAGTTTCAAGAGAAT+TGG - chr7.3:83203066-83203085 MS.gene44654:CDS 25.0%
! TCAATAGGAACAATATTCAT+TGG - chr7.3:83202999-83203018 MS.gene44654:CDS 25.0%
! TTCTTCAAATGTTAATGCAA+TGG - chr7.3:83203411-83203430 MS.gene44654:CDS 25.0%
!! TCAACATTTGATGATAATGA+TGG + chr7.3:83203471-83203490 None:intergenic 25.0%
AAATGTTAATGCAATGGAGA+TGG - chr7.3:83203417-83203436 MS.gene44654:CDS 30.0%
ACTTGGTACTAATTACCAAT+GGG - chr7.3:83203255-83203274 MS.gene44654:CDS 30.0%
AGTGAACTCTTGTTCTTTAT+TGG + chr7.3:83203590-83203609 None:intergenic 30.0%
ATGATCTTCAAGATAGGTTA+GGG - chr7.3:83202819-83202838 MS.gene44654:CDS 30.0%
ATTCTTGGATCTTTCAATCT+TGG + chr7.3:83202686-83202705 None:intergenic 30.0%
CTTCAAGTCAAAATTCTTCT+TGG - chr7.3:83203032-83203051 MS.gene44654:CDS 30.0%
GATTGAGAGATAGAAGAATA+AGG - chr7.3:83202768-83202787 MS.gene44654:CDS 30.0%
GCTGAAAATTCTTCTCAATT+GGG - chr7.3:83203295-83203314 MS.gene44654:CDS 30.0%
GTAGGAACAAATTTCAACTT+TGG - chr7.3:83202932-83202951 MS.gene44654:CDS 30.0%
TATGATCTTCAAGATAGGTT+AGG - chr7.3:83202818-83202837 MS.gene44654:CDS 30.0%
TCATAAAGTTGAATAGCTGT+TGG + chr7.3:83202803-83202822 None:intergenic 30.0%
TGCTGAAAATTCTTCTCAAT+TGG - chr7.3:83203294-83203313 MS.gene44654:CDS 30.0%
TTATTAAATCCTATGCCACT+TGG - chr7.3:83203238-83203257 MS.gene44654:CDS 30.0%
TTGTTGAGAGAATTAGTCAT+TGG + chr7.3:83203517-83203536 None:intergenic 30.0%
TTGTTGAGTTCATTAGAAGA+TGG + chr7.3:83202962-83202981 None:intergenic 30.0%
TTTGGAAAAACATTTGCAGT+AGG + chr7.3:83203172-83203191 None:intergenic 30.0%
! AAGTTGAAATTTGTTCCTAC+AGG + chr7.3:83202932-83202951 None:intergenic 30.0%
! AGAAGAATTTTCAGCATCAT+TGG + chr7.3:83203290-83203309 None:intergenic 30.0%
! AGATTTTCCATTGAAACAAG+AGG - chr7.3:83202583-83202602 MS.gene44654:CDS 30.0%
! TTTGATGATAATGATGGTTG+TGG + chr7.3:83203465-83203484 None:intergenic 30.0%
!!! TCTGCTGTTTTGTAGAAATT+TGG + chr7.3:83203557-83203576 None:intergenic 30.0%
AACCAATCAACAACTTTGCT+AGG + chr7.3:83202854-83202873 None:intergenic 35.0%
AACCTAGCAAAGTTGTTGAT+TGG - chr7.3:83202849-83202868 MS.gene44654:CDS 35.0%
ACAGGTGGAAAATTAGGTAA+TGG + chr7.3:83202914-83202933 None:intergenic 35.0%
AGCAAAGTATGCACAATAAG+AGG - chr7.3:83202746-83202765 MS.gene44654:CDS 35.0%
AGGAACAATATTCATTGGGA+AGG - chr7.3:83203004-83203023 MS.gene44654:CDS 35.0%
CACTTGGTACTAATTACCAA+TGG - chr7.3:83203254-83203273 MS.gene44654:CDS 35.0%
CCAAATCACCCTTCATTTAT+TGG - chr7.3:83203214-83203233 MS.gene44654:CDS 35.0%
CCAATAAATGAAGGGTGATT+TGG + chr7.3:83203217-83203236 None:intergenic 35.0%
CCAATGTTGTCTTTATCAAC+TGG - chr7.3:83203346-83203365 MS.gene44654:CDS 35.0%
CTTGATACTCTGACAATTCT+TGG + chr7.3:83202701-83202720 None:intergenic 35.0%
CTTGGTACTAATTACCAATG+GGG - chr7.3:83203256-83203275 MS.gene44654:CDS 35.0%
TAATTAGTACCAAGTGGCAT+AGG + chr7.3:83203250-83203269 None:intergenic 35.0%
TAGAAATTTGGCCAAACTGA+AGG + chr7.3:83203545-83203564 None:intergenic 35.0%
TCTTCTCAATTGGGAAACAA+TGG - chr7.3:83203304-83203323 MS.gene44654:CDS 35.0%
! CATTGGTAATTAGTACCAAG+TGG + chr7.3:83203256-83203275 None:intergenic 35.0%
! GTTGATAAAGACAACATTGG+TGG + chr7.3:83203346-83203365 None:intergenic 35.0%
! TGATGAACCTCTTGTTTCAA+TGG + chr7.3:83202593-83202612 None:intergenic 35.0%
! TTACCTAATTTTCCACCTGT+AGG - chr7.3:83202914-83202933 MS.gene44654:CDS 35.0%
! TTGAAATTTGTTCCTACAGG+TGG + chr7.3:83202929-83202948 None:intergenic 35.0%
!! CCAGTTGATAAAGACAACAT+TGG + chr7.3:83203349-83203368 None:intergenic 35.0%
AATTTGGCCAAACTGAAGGA+TGG + chr7.3:83203541-83203560 None:intergenic 40.0%
CAACAATCCATCCTTCAGTT+TGG - chr7.3:83203531-83203550 MS.gene44654:CDS 40.0%
GTTCCTACAGGTGGAAAATT+AGG + chr7.3:83202920-83202939 None:intergenic 40.0%
! GGTGGAAAATTAGGTAATGG+TGG + chr7.3:83202911-83202930 None:intergenic 40.0%
!! AGAGTATCAAGAGCTTTTGG+TGG - chr7.3:83202710-83202729 MS.gene44654:CDS 40.0%
!! GTCAGAGTATCAAGAGCTTT+TGG - chr7.3:83202707-83202726 MS.gene44654:CDS 40.0%
GAAGCTAAGGAGCTTGATGA+TGG + chr7.3:83202653-83202672 None:intergenic 45.0%
GCTCCTTAGCTTCATCACAA+TGG - chr7.3:83202660-83202679 MS.gene44654:CDS 45.0%
GGTACTAATTACCAATGGGG+AGG - chr7.3:83203259-83203278 MS.gene44654:CDS 45.0%
TGGCCATTGTGATGAAGCTA+AGG + chr7.3:83202666-83202685 None:intergenic 45.0%
!! CATTGGAAGTACCTCCCCAT+TGG + chr7.3:83203273-83203292 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 83202563 83203618 83202563 ID=MS.gene44654
chr7.3 mRNA 83202563 83203618 83202563 ID=MS.gene44654.t1;Parent=MS.gene44654
chr7.3 exon 83202563 83203618 83202563 ID=MS.gene44654.t1.exon1;Parent=MS.gene44654.t1
chr7.3 CDS 83202563 83203618 83202563 ID=cds.MS.gene44654.t1;Parent=MS.gene44654.t1
Gene Sequence

>MS.gene44654

ATGAATAAGAATTCAAAAGAAGATTTTCCATTGAAACAAGAGGTTCATCAAAATTCAAACGATCATGAAAAAGAAAAGGCTAATTCACCATCATCAAGCTCCTTAGCTTCATCACAATGGCCAAGATTGAAAGATCCAAGAATTGTCAGAGTATCAAGAGCTTTTGGTGGAAAAGATAGACATAGCAAAGTATGCACAATAAGAGGATTGAGAGATAGAAGAATAAGGCTTTCAGTACCAACAGCTATTCAACTTTATGATCTTCAAGATAGGTTAGGGCTTAATCAACCTAGCAAAGTTGTTGATTGGTTGCTCAATGCAGCTAAACATGACATTGATCAACTTCCACCATTACCTAATTTTCCACCTGTAGGAACAAATTTCAACTTTGGTTTTCCATCTTCTAATGAACTCAACAATCAACAATTGTTCAATTTCAATAGGAACAATATTCATTGGGAAGGTTCAACTTCAAGTCAAAATTCTTCTTGGAAATCAAACCCTAAAGAAGTTTCAAGAGAATTGGTTAATGATAAACCAAATTACTTGATGAATAGTACTCAAGAAGATGAAAATATTAACAAGCAAGGAAGCAATATTGAAGTTCCTACTGCAAATGTTTTTCCAAATAATAACAATATGTTACAAAGACCAAATCACCCTTCATTTATTGGTTTATTAAATCCTATGCCACTTGGTACTAATTACCAATGGGGAGGTACTTCCAATGATGCTGAAAATTCTTCTCAATTGGGAAACAATGGCTTTATTACATCTTTGCCACCAATGTTGTCTTTATCAACTGGAAATTCTTCTCAAATGATGTTAGGAACAACACAATCATATTTTTCTTCAAATGTTAATGCAATGGAGATGGATCATCATCATCAAAGAGAAATCCACAACCATCATTATCATCAAATGTTGAGTTCATCAAGTTCTTCTCAGAATCCAATGACTAATTCTCTCAACAATCCATCCTTCAGTTTGGCCAAATTTCTACAAAACAGCAGAGAAAATTCATCCAATAAAGAACAAGAGTTCACTACAAAGTGA

Protein sequence

>MS.gene44654.t1

MNKNSKEDFPLKQEVHQNSNDHEKEKANSPSSSSLASSQWPRLKDPRIVRVSRAFGGKDRHSKVCTIRGLRDRRIRLSVPTAIQLYDLQDRLGLNQPSKVVDWLLNAAKHDIDQLPPLPNFPPVGTNFNFGFPSSNELNNQQLFNFNRNNIHWEGSTSSQNSSWKSNPKEVSRELVNDKPNYLMNSTQEDENINKQGSNIEVPTANVFPNNNNMLQRPNHPSFIGLLNPMPLGTNYQWGGTSNDAENSSQLGNNGFITSLPPMLSLSTGNSSQMMLGTTQSYFSSNVNAMEMDHHHQREIHNHHYHQMLSSSSSSQNPMTNSLNNPSFSLAKFLQNSRENSSNKEQEFTTK