Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44876.t1 | XP_003615604.1 | 100 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 3.90E-26 | 127.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44876.t1 | G7K6C4 | 100.0 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 2.8e-26 | 127.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene44876 | 0.803345 | 3.64E-49 | -1.69E-46 |
| MS.gene049151 | MS.gene44876 | 0.801272 | 9.74E-49 | -1.69E-46 |
| MS.gene049153 | MS.gene44876 | 0.806358 | 8.55E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene44876 | 0.857521 | 1.54E-62 | -1.69E-46 |
| MS.gene049369 | MS.gene44876 | 0.81843 | 1.97E-52 | -1.69E-46 |
| MS.gene049491 | MS.gene44876 | 0.820224 | 7.68E-53 | -1.69E-46 |
| MS.gene049758 | MS.gene44876 | 0.854079 | 1.56E-61 | -1.69E-46 |
| MS.gene049910 | MS.gene44876 | 0.813021 | 3.16E-51 | -1.69E-46 |
| MS.gene049915 | MS.gene44876 | 0.805245 | 1.47E-49 | -1.69E-46 |
| MS.gene050128 | MS.gene44876 | 0.857563 | 1.50E-62 | -1.69E-46 |
| MS.gene050301 | MS.gene44876 | 0.85951 | 3.94E-63 | -1.69E-46 |
| MS.gene050397 | MS.gene44876 | 0.810672 | 1.03E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene44876 | 0.8054 | 1.36E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene44876 | 0.830532 | 2.79E-55 | -1.69E-46 |
| MS.gene050971 | MS.gene44876 | 0.815733 | 7.95E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene44876 | 0.816089 | 6.62E-52 | -1.69E-46 |
| MS.gene051360 | MS.gene44876 | 0.836135 | 1.12E-56 | -1.69E-46 |
| MS.gene051605 | MS.gene44876 | 0.81312 | 3.01E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene44876 | 0.816917 | 4.32E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene44876 | 0.807456 | 5.01E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene44876 | 0.846532 | 2.05E-59 | -1.69E-46 |
| MS.gene052178 | MS.gene44876 | 0.801195 | 1.01E-48 | -1.69E-46 |
| MS.gene052188 | MS.gene44876 | 0.886191 | 4.24E-72 | -1.69E-46 |
| MS.gene052253 | MS.gene44876 | 0.842033 | 3.31E-58 | -1.69E-46 |
| MS.gene052310 | MS.gene44876 | 0.833387 | 5.49E-56 | -1.69E-46 |
| MS.gene052340 | MS.gene44876 | 0.894351 | 2.68E-75 | -1.69E-46 |
| MS.gene052485 | MS.gene44876 | 0.830372 | 3.05E-55 | -1.69E-46 |
| MS.gene053073 | MS.gene44876 | 0.82304 | 1.72E-53 | -1.69E-46 |
| MS.gene05316 | MS.gene44876 | 0.851928 | 6.45E-61 | -1.69E-46 |
| MS.gene053209 | MS.gene44876 | 0.83866 | 2.52E-57 | -1.69E-46 |
| MS.gene053557 | MS.gene44876 | 0.864029 | 1.63E-64 | -1.69E-46 |
| MS.gene053559 | MS.gene44876 | 0.815367 | 9.59E-52 | -1.69E-46 |
| MS.gene053577 | MS.gene44876 | 0.80803 | 3.78E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene44876 | 0.831734 | 1.41E-55 | -1.69E-46 |
| MS.gene054123 | MS.gene44876 | 0.820293 | 7.40E-53 | -1.69E-46 |
| MS.gene054349 | MS.gene44876 | 0.824156 | 9.40E-54 | -1.69E-46 |
| MS.gene054542 | MS.gene44876 | 0.818606 | 1.80E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene44876 | 0.826714 | 2.33E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene44876 | 0.85693 | 2.31E-62 | -1.69E-46 |
| MS.gene055277 | MS.gene44876 | 0.800218 | 1.60E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene44876 | 0.819457 | 1.15E-52 | -1.69E-46 |
| MS.gene055859 | MS.gene44876 | 0.809795 | 1.59E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene44876 | 0.801003 | 1.11E-48 | -1.69E-46 |
| MS.gene056314 | MS.gene44876 | 0.801311 | 9.56E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene44876 | 0.804206 | 2.41E-49 | -1.69E-46 |
| MS.gene056410 | MS.gene44876 | 0.804945 | 1.69E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene44876 | 0.809467 | 1.86E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene44876 | 0.825027 | 5.86E-54 | -1.69E-46 |
| MS.gene056612 | MS.gene44876 | 0.803801 | 2.93E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene44876 | 0.85177 | 7.15E-61 | -1.69E-46 |
| MS.gene056719 | MS.gene44876 | 0.827011 | 1.98E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene44876 | 0.820429 | 6.89E-53 | -1.69E-46 |
| MS.gene057396 | MS.gene44876 | 0.807859 | 4.11E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene44876 | 0.861729 | 8.36E-64 | -1.69E-46 |
| MS.gene057449 | MS.gene44876 | 0.845284 | 4.47E-59 | -1.69E-46 |
| MS.gene057450 | MS.gene44876 | 0.866737 | 2.29E-65 | -1.69E-46 |
| MS.gene057796 | MS.gene44876 | 0.846307 | 2.36E-59 | -1.69E-46 |
| MS.gene058002 | MS.gene44876 | 0.846266 | 2.42E-59 | -1.69E-46 |
| MS.gene058007 | MS.gene44876 | 0.812554 | 4.00E-51 | -1.69E-46 |
| MS.gene058082 | MS.gene44876 | 0.849942 | 2.34E-60 | -1.69E-46 |
| MS.gene05811 | MS.gene44876 | 0.849085 | 4.06E-60 | -1.69E-46 |
| MS.gene058142 | MS.gene44876 | 0.815556 | 8.70E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene44876 | 0.817966 | 2.51E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene44876 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene44876 | 0.822403 | 2.41E-53 | -1.69E-46 |
| MS.gene058787 | MS.gene44876 | 0.809055 | 2.29E-50 | -1.69E-46 |
| MS.gene058845 | MS.gene44876 | 0.805128 | 1.55E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene44876 | 0.843764 | 1.15E-58 | -1.69E-46 |
| MS.gene058949 | MS.gene44876 | 0.806504 | 7.97E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene44876 | 0.845846 | 3.15E-59 | -1.69E-46 |
| MS.gene059285 | MS.gene44876 | 0.854422 | 1.24E-61 | -1.69E-46 |
| MS.gene059294 | MS.gene44876 | 0.801039 | 1.09E-48 | -1.69E-46 |
| MS.gene059342 | MS.gene44876 | 0.813732 | 2.21E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene44876 | 0.844016 | 9.82E-59 | -1.69E-46 |
| MS.gene059518 | MS.gene44876 | 0.816686 | 4.87E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene44876 | 0.852892 | 3.43E-61 | -1.69E-46 |
| MS.gene059676 | MS.gene44876 | 0.829994 | 3.77E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene44876 | 0.856163 | 3.87E-62 | -1.69E-46 |
| MS.gene059692 | MS.gene44876 | 0.866472 | 2.78E-65 | -1.69E-46 |
| MS.gene059695 | MS.gene44876 | 0.863496 | 2.39E-64 | -1.69E-46 |
| MS.gene059708 | MS.gene44876 | 0.836348 | 9.86E-57 | -1.69E-46 |
| MS.gene059711 | MS.gene44876 | 0.805033 | 1.62E-49 | -1.69E-46 |
| MS.gene059788 | MS.gene44876 | 0.804223 | 2.40E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene44876 | 0.8512 | 1.04E-60 | -1.69E-46 |
| MS.gene05984 | MS.gene44876 | 0.862666 | 4.31E-64 | -1.69E-46 |
| MS.gene059894 | MS.gene44876 | 0.857834 | 1.25E-62 | -1.69E-46 |
| MS.gene059899 | MS.gene44876 | 0.826104 | 3.26E-54 | -1.69E-46 |
| MS.gene060247 | MS.gene44876 | 0.8184 | 2.00E-52 | -1.69E-46 |
| MS.gene06037 | MS.gene44876 | 0.813122 | 3.00E-51 | -1.69E-46 |
| MS.gene060495 | MS.gene44876 | 0.850264 | 1.90E-60 | -1.69E-46 |
| MS.gene060986 | MS.gene44876 | 0.806112 | 9.63E-50 | -1.69E-46 |
| MS.gene061041 | MS.gene44876 | 0.801265 | 9.77E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene44876 | 0.866837 | 2.13E-65 | -1.69E-46 |
| MS.gene061051 | MS.gene44876 | -0.833514 | 5.10E-56 | -1.69E-46 |
| MS.gene061154 | MS.gene44876 | 0.803133 | 4.03E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44876.t1 | MTR_5g069990 | 93.750 | 112 | 1 | 1 | 1 | 112 | 1 | 106 | 6.10e-69 | 202 |
| MS.gene44876.t1 | MTR_3g462990 | 52.941 | 102 | 26 | 5 | 1 | 100 | 1 | 82 | 8.41e-22 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44876.t1 | AT3G47070 | 50.000 | 64 | 29 | 2 | 1 | 64 | 1 | 61 | 1.04e-13 | 62.0 |
Find 27 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAAGAAGAAGGGTGGTTT+TGG | 0.266622 | 5.2:-59254746 | MS.gene44876:CDS |
| CTTCCTGTTGTTGGAACCAT+TGG | 0.379541 | 5.2:+59255019 | None:intergenic |
| GCAAACACTCTTCCTGTTGT+TGG | 0.394308 | 5.2:+59255010 | None:intergenic |
| TCTTCAACCTTCTTGAGAAT+AGG | 0.417605 | 5.2:+59254875 | None:intergenic |
| TTGCCAATGGTTCCAACAAC+AGG | 0.422646 | 5.2:-59255022 | MS.gene44876:CDS |
| TTCTATCACCATGTTGCCAA+TGG | 0.469167 | 5.2:-59255035 | MS.gene44876:CDS |
| GGAAGAGTGTTTGCAGCTAC+AGG | 0.472522 | 5.2:-59255001 | MS.gene44876:CDS |
| GGCAGTAGCAAGCAAGAGAA+AGG | 0.509106 | 5.2:-59254956 | MS.gene44876:CDS |
| AGAGGTACTACTAGTGGTAA+AGG | 0.534650 | 5.2:-59254806 | MS.gene44876:CDS |
| AGAGGTACTACTAGTGGTAG+AGG | 0.535016 | 5.2:-59254824 | MS.gene44876:CDS |
| GGTAGTTCCACAAAAGAAGA+AGG | 0.536969 | 5.2:-59254756 | MS.gene44876:CDS |
| ACAGGTGCAAAGGGTACTAC+AGG | 0.544200 | 5.2:-59254983 | MS.gene44876:CDS |
| AACTGATCCTATTCTCAAGA+AGG | 0.548104 | 5.2:-59254882 | MS.gene44876:CDS |
| TTTGCAGCTACAGGTGCAAA+GGG | 0.555094 | 5.2:-59254992 | MS.gene44876:CDS |
| GTTTGCAGCTACAGGTGCAA+AGG | 0.565663 | 5.2:-59254993 | MS.gene44876:CDS |
| GCAAAGGGTACTACAGGTGG+TGG | 0.567835 | 5.2:-59254977 | MS.gene44876:CDS |
| GAAGAGAAGAATAATAGTAG+AGG | 0.604921 | 5.2:-59254857 | MS.gene44876:CDS |
| TGTTGGAACCATTGGCAACA+TGG | 0.606092 | 5.2:+59255027 | None:intergenic |
| AGTGGTAAAGGCACTGCTAG+TGG | 0.607971 | 5.2:-59254794 | MS.gene44876:CDS |
| AGTAGAGGCACTACTAGTAG+AGG | 0.624915 | 5.2:-59254842 | MS.gene44876:CDS |
| GGTAAAGGCACTGCTAGTGG+TGG | 0.640988 | 5.2:-59254791 | MS.gene44876:CDS |
| GTTCCACAAAAGAAGAAGGG+TGG | 0.647995 | 5.2:-59254752 | MS.gene44876:CDS |
| ACTAGTAGAGGTACTACTAG+TGG | 0.652621 | 5.2:-59254830 | MS.gene44876:CDS |
| GTAGTTCCACAAAAGAAGAA+GGG | 0.659042 | 5.2:-59254755 | MS.gene44876:CDS |
| AGTGGTAGAGGTACTACTAG+TGG | 0.660105 | 5.2:-59254812 | MS.gene44876:CDS |
| GGTGCAAAGGGTACTACAGG+TGG | 0.681783 | 5.2:-59254980 | MS.gene44876:CDS |
| TAGTGGTGGCAAGAACTCTG+TGG | 0.703573 | 5.2:-59254777 | MS.gene44876:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGGAGGATTTTTTAACA+AGG | - | chr5.2:59255035-59255054 | MS.gene44876:CDS | 25.0% |
| GAAGAGAAGAATAATAGTAG+AGG | - | chr5.2:59254907-59254926 | MS.gene44876:CDS | 30.0% | |
| AACTGATCCTATTCTCAAGA+AGG | - | chr5.2:59254882-59254901 | MS.gene44876:CDS | 35.0% | |
| GTAGTTCCACAAAAGAAGAA+GGG | - | chr5.2:59255009-59255028 | MS.gene44876:CDS | 35.0% | |
| TCTTCAACCTTCTTGAGAAT+AGG | + | chr5.2:59254892-59254911 | None:intergenic | 35.0% | |
| ! | CAAGCAAGAGAAAGGTTTTT+GGG | - | chr5.2:59254816-59254835 | MS.gene44876:CDS | 35.0% |
| !!! | AAGAGAAAGGTTTTTGGGAT+TGG | - | chr5.2:59254821-59254840 | MS.gene44876:CDS | 35.0% |
| GGTAGTTCCACAAAAGAAGA+AGG | - | chr5.2:59255008-59255027 | MS.gene44876:CDS | 40.0% | |
| TTCTATCACCATGTTGCCAA+TGG | - | chr5.2:59254729-59254748 | MS.gene44876:CDS | 40.0% | |
| ! | AAACCACCCTTCTTCTTTTG+TGG | + | chr5.2:59255018-59255037 | None:intergenic | 40.0% |
| ! | CAAAAGAAGAAGGGTGGTTT+TGG | - | chr5.2:59255018-59255037 | MS.gene44876:CDS | 40.0% |
| ! | GCAAGCAAGAGAAAGGTTTT+TGG | - | chr5.2:59254815-59254834 | MS.gene44876:CDS | 40.0% |
| !! | ACTAGTAGAGGTACTACTAG+TGG | - | chr5.2:59254934-59254953 | MS.gene44876:CDS | 40.0% |
| !! | AGAGGTACTACTAGTGGTAA+AGG | - | chr5.2:59254958-59254977 | MS.gene44876:CDS | 40.0% |
| !!! | GGTTTTTGGGATTGGATTGT+TGG | - | chr5.2:59254829-59254848 | MS.gene44876:CDS | 40.0% |
| !!! | TTTTGGGATTGGATTGTTGG+TGG | - | chr5.2:59254832-59254851 | MS.gene44876:CDS | 40.0% |
| CTTCCTGTTGTTGGAACCAT+TGG | + | chr5.2:59254748-59254767 | None:intergenic | 45.0% | |
| GCAAACACTCTTCCTGTTGT+TGG | + | chr5.2:59254757-59254776 | None:intergenic | 45.0% | |
| GTTCCACAAAAGAAGAAGGG+TGG | - | chr5.2:59255012-59255031 | MS.gene44876:CDS | 45.0% | |
| TGTTGGAACCATTGGCAACA+TGG | + | chr5.2:59254740-59254759 | None:intergenic | 45.0% | |
| TTGCCAATGGTTCCAACAAC+AGG | - | chr5.2:59254742-59254761 | MS.gene44876:CDS | 45.0% | |
| TTTGCAGCTACAGGTGCAAA+GGG | - | chr5.2:59254772-59254791 | MS.gene44876:CDS | 45.0% | |
| !! | AGAGGTACTACTAGTGGTAG+AGG | - | chr5.2:59254940-59254959 | MS.gene44876:CDS | 45.0% |
| !! | AGTAGAGGCACTACTAGTAG+AGG | - | chr5.2:59254922-59254941 | MS.gene44876:CDS | 45.0% |
| !! | AGTGGTAGAGGTACTACTAG+TGG | - | chr5.2:59254952-59254971 | MS.gene44876:CDS | 45.0% |
| !!! | AAGAAGAAGGGTGGTTTTGG+AGG | - | chr5.2:59255021-59255040 | MS.gene44876:CDS | 45.0% |
| GGCAGTAGCAAGCAAGAGAA+AGG | - | chr5.2:59254808-59254827 | MS.gene44876:CDS | 50.0% | |
| GTTTGCAGCTACAGGTGCAA+AGG | - | chr5.2:59254771-59254790 | MS.gene44876:CDS | 50.0% | |
| TAGTGGTGGCAAGAACTCTG+TGG | - | chr5.2:59254987-59255006 | MS.gene44876:CDS | 50.0% | |
| !! | ACAGGTGCAAAGGGTACTAC+AGG | - | chr5.2:59254781-59254800 | MS.gene44876:CDS | 50.0% |
| !! | AGTGGTAAAGGCACTGCTAG+TGG | - | chr5.2:59254970-59254989 | MS.gene44876:CDS | 50.0% |
| !! | GGAAGAGTGTTTGCAGCTAC+AGG | - | chr5.2:59254763-59254782 | MS.gene44876:CDS | 50.0% |
| !! | GCAAAGGGTACTACAGGTGG+TGG | - | chr5.2:59254787-59254806 | MS.gene44876:CDS | 55.0% |
| !! | GGTAAAGGCACTGCTAGTGG+TGG | - | chr5.2:59254973-59254992 | MS.gene44876:CDS | 55.0% |
| !! | GGTGCAAAGGGTACTACAGG+TGG | - | chr5.2:59254784-59254803 | MS.gene44876:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 59254724 | 59255062 | 59254724 | ID=MS.gene44876 |
| chr5.2 | mRNA | 59254724 | 59255062 | 59254724 | ID=MS.gene44876.t1;Parent=MS.gene44876 |
| chr5.2 | exon | 59254724 | 59255062 | 59254724 | ID=MS.gene44876.t1.exon1;Parent=MS.gene44876.t1 |
| chr5.2 | CDS | 59254724 | 59255062 | 59254724 | ID=cds.MS.gene44876.t1;Parent=MS.gene44876.t1 |
| Gene Sequence |
| Protein sequence |
