AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48285


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTCTCAGTATATTAAT+TGG 0.148489 8.1:+20316810 MS.gene48285:CDS
TTTCCTGAATTCTTGTAATT+TGG 0.171860 8.1:-20316606 None:intergenic
GGTGTTAACTTTGCTGTTGC+TGG 0.263157 8.1:+20315966 MS.gene48285:CDS
CAGTGTCTCAGGAGCTTTAC+AGG 0.269526 8.1:+20316238 MS.gene48285:CDS
GCTGAAGGTCCAAGTGGTTT+TGG 0.306391 8.1:+20315789 MS.gene48285:CDS
AATGATTATGCTTACACTCT+TGG 0.315837 8.1:+20316173 MS.gene48285:CDS
TAAACCATGAATTCTTTGTT+AGG 0.329985 8.1:+20316000 MS.gene48285:CDS
AAAAGATCAAATGATACTTT+TGG 0.334241 8.1:+20315945 MS.gene48285:CDS
TCTTACCGAAGCTATGTATA+AGG 0.339713 8.1:+20316844 MS.gene48285:CDS
TATTCCTAACAAAGAATTCA+TGG 0.348303 8.1:-20316004 None:intergenic
CAGTCTCAGAAGATACTATA+AGG 0.400671 8.1:+20316201 MS.gene48285:CDS
CCCTTCTCAGTATATTAATT+GGG 0.408026 8.1:+20316811 MS.gene48285:CDS
GTGCAAGGTCATCCACCAAC+AGG 0.409203 8.1:+20316476 MS.gene48285:CDS
TTCAAGAAAATTTACGCCTT+CGG 0.409856 8.1:+20315732 MS.gene48285:CDS
ATGAAGAATCCAAGCAAGTA+TGG 0.412451 8.1:+20316686 MS.gene48285:CDS
TAAGGTTGTGATTGTTGCTT+AGG 0.412894 8.1:-20316574 None:intergenic
GTTTCTAATATGTTTCTTCA+AGG 0.421614 8.1:+20316869 MS.gene48285:CDS
CTTGCAATGTCCAGTGTCTC+AGG 0.422400 8.1:+20316227 MS.gene48285:CDS
CCTAAGTCGTCTCTGTCATC+TGG 0.426751 8.1:-20316530 None:intergenic
AAGCCAAATTACAAGAATTC+AGG 0.428938 8.1:+20316603 MS.gene48285:CDS
GTAATTTGGCTTGCAGCATA+AGG 0.433254 8.1:-20316592 None:intergenic
TTCAGTGTCTGCTGTGGATC+AGG 0.434569 8.1:+20316722 MS.gene48285:CDS
TCTTTGAATCCATACTTGCT+TGG 0.457241 8.1:-20316695 None:intergenic
TCCATCAGAATATCTGTTTG+TGG 0.461336 8.1:-20315856 None:intergenic
GCAAACACAGTAAAGTTGTA+AGG 0.470362 8.1:-20316752 None:intergenic
CTAAATACATTGTCAGTGTA+AGG 0.474491 8.1:-20316502 None:intergenic
CTGTAAAGCTCCTGAGACAC+TGG 0.475224 8.1:-20316237 None:intergenic
CTAGTGCAGACAGTAGCATT+AGG 0.483410 8.1:-20316788 None:intergenic
TTTACTGTGTTTGCTACTTG+TGG 0.486375 8.1:+20316761 MS.gene48285:CDS
CCCAATTAATATACTGAGAA+GGG 0.489096 8.1:-20316811 None:intergenic
ATTGTGGTTTAATAAGTACT+TGG 0.491945 8.1:+20316070 MS.gene48285:CDS
AACAACCTTATACATAGCTT+CGG 0.507341 8.1:-20316849 None:intergenic
ACCTTGCACAACTAGGTACT+TGG 0.509796 8.1:-20316462 None:intergenic
CTATTCAAACTCAGATATTG+TGG 0.510682 8.1:+20316054 MS.gene48285:CDS
AATCAACTCCTTGACAACCT+TGG 0.512025 8.1:-20316097 None:intergenic
TTGAAGAAAGTTGTTCCATA+TGG 0.515073 8.1:-20315828 None:intergenic
GATACATGGCCAAAACCACT+TGG 0.515353 8.1:-20315798 None:intergenic
TGACGATAAGGAGGAAGATA+AGG 0.521221 8.1:-20315918 None:intergenic
AGTGTAAGGCACCCTGTTGG+TGG 0.525307 8.1:-20316488 None:intergenic
TCTCAGTATATTAATTGGGA+TGG 0.526757 8.1:+20316815 MS.gene48285:CDS
TTTGCAGTCATTGCTTGAGA+AGG 0.530230 8.1:+20316436 MS.gene48285:intron
CCAGATGACAGAGACGACTT+AGG 0.531180 8.1:+20316530 MS.gene48285:CDS
TTGCAGTCATTGCTTGAGAA+GGG 0.533529 8.1:+20316437 MS.gene48285:intron
TCCCAATTAATATACTGAGA+AGG 0.543751 8.1:-20316812 None:intergenic
CATGTATCAAACTCACCATA+TGG 0.543972 8.1:+20315813 MS.gene48285:CDS
GCATAAACTATGACTGCATG+TGG 0.551677 8.1:-20316635 None:intergenic
CAGAATATCTGTTTGTGGAA+TGG 0.551682 8.1:-20315851 None:intergenic
AATAAGTACTTGGAGAGCCA+AGG 0.559798 8.1:+20316080 MS.gene48285:CDS
TTGGAGAGCCAAGGTTGTCA+AGG 0.574397 8.1:+20316089 MS.gene48285:CDS
GTGGATGACCTTGCACAACT+AGG 0.575276 8.1:-20316469 None:intergenic
TTGGATTCTTCATCACAGTA+CGG 0.581211 8.1:-20316676 None:intergenic
CATATGGTGAGTTTGATACA+TGG 0.583710 8.1:-20315812 None:intergenic
TCCACAAACAGATATTCTGA+TGG 0.587630 8.1:+20315855 MS.gene48285:CDS
GGAGACTCTTTCACAGACAC+AGG 0.589716 8.1:+20315753 MS.gene48285:CDS
GGCAACACACACAACGCTGA+AGG 0.600278 8.1:+20315774 MS.gene48285:CDS
GTCTGTGAAAGAGTCTCCGA+AGG 0.622353 8.1:-20315748 None:intergenic
AAGTCGTCTCTGTCATCTGG+AGG 0.624671 8.1:-20316527 None:intergenic
GCCAAGTACCTAGTTGTGCA+AGG 0.625395 8.1:+20316461 MS.gene48285:CDS
GTCAGTGTAAGGCACCCTGT+TGG 0.629703 8.1:-20316491 None:intergenic
TGCAAGGTCATCCACCAACA+GGG 0.656181 8.1:+20316477 MS.gene48285:CDS
TTGGAAAACAAAGAGAGACA+TGG 0.662787 8.1:+20316923 MS.gene48285:CDS
AACACAGTAAAGTTGTAAGG+AGG 0.674046 8.1:-20316749 None:intergenic
GATATGTTCAGTGTCTGCTG+TGG 0.678417 8.1:+20316716 MS.gene48285:CDS
CACAACGCTGAAGGTCCAAG+TGG 0.686660 8.1:+20315783 MS.gene48285:CDS
ATCTGAGTTTGAATAGACTG+AGG 0.688837 8.1:-20316047 None:intergenic
CATAAACTATGACTGCATGT+GGG 0.692524 8.1:-20316634 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATGGTTTATTTGT+TGG + chr8.1:20316312-20316331 MS.gene48285:intron 15.0%
!! AAAAATGGGAGTTATATATA+TGG + chr8.1:20316300-20316319 MS.gene48285:intron 20.0%
!!! AAAAGATCAAATGATACTTT+TGG + chr8.1:20315945-20315964 MS.gene48285:CDS 20.0%
!!! TTTTCCAACAAAAAATCAAA+TGG - chr8.1:20316911-20316930 None:intergenic 20.0%
! AAGATTAGTGCACATAAAAA+TGG + chr8.1:20316285-20316304 MS.gene48285:intron 25.0%
! AGATTAGTGCACATAAAAAT+GGG + chr8.1:20316286-20316305 MS.gene48285:intron 25.0%
! ATTGTGGTTTAATAAGTACT+TGG + chr8.1:20316070-20316089 MS.gene48285:CDS 25.0%
! GTTTCTAATATGTTTCTTCA+AGG + chr8.1:20316869-20316888 MS.gene48285:CDS 25.0%
! TAAACCATGAATTCTTTGTT+AGG + chr8.1:20316000-20316019 MS.gene48285:CDS 25.0%
! TATTCCTAACAAAGAATTCA+TGG - chr8.1:20316007-20316026 None:intergenic 25.0%
! TTTCCTGAATTCTTGTAATT+TGG - chr8.1:20316609-20316628 None:intergenic 25.0%
!!! ATTTTGATGAGACTCTTTTT+TGG + chr8.1:20316132-20316151 MS.gene48285:CDS 25.0%
AACAACCTTATACATAGCTT+CGG - chr8.1:20316852-20316871 None:intergenic 30.0%
AAGCCAAATTACAAGAATTC+AGG + chr8.1:20316603-20316622 MS.gene48285:CDS 30.0%
AATGATTATGCTTACACTCT+TGG + chr8.1:20316173-20316192 MS.gene48285:CDS 30.0%
ACAAAAAATCAAATGGAGGT+TGG - chr8.1:20316904-20316923 None:intergenic 30.0%
CCCAATTAATATACTGAGAA+GGG - chr8.1:20316814-20316833 None:intergenic 30.0%
CCCTTCTCAGTATATTAATT+GGG + chr8.1:20316811-20316830 MS.gene48285:CDS 30.0%
CTAAATACATTGTCAGTGTA+AGG - chr8.1:20316505-20316524 None:intergenic 30.0%
CTATTCAAACTCAGATATTG+TGG + chr8.1:20316054-20316073 MS.gene48285:CDS 30.0%
GAAAAAAAATCTCTAGACCA+AGG - chr8.1:20316345-20316364 None:intergenic 30.0%
TCCAACAAAAAATCAAATGG+AGG - chr8.1:20316908-20316927 None:intergenic 30.0%
TCCCAATTAATATACTGAGA+AGG - chr8.1:20316815-20316834 None:intergenic 30.0%
TCTCAGTATATTAATTGGGA+TGG + chr8.1:20316815-20316834 MS.gene48285:CDS 30.0%
TTCAAGAAAATTTACGCCTT+CGG + chr8.1:20315732-20315751 MS.gene48285:CDS 30.0%
TTGAAGAAAGTTGTTCCATA+TGG - chr8.1:20315831-20315850 None:intergenic 30.0%
! AAGGCGTAAATTTTCTTGAA+AGG - chr8.1:20315732-20315751 None:intergenic 30.0%
! TATGGTTTATTTGTTGGTCT+TGG + chr8.1:20316318-20316337 MS.gene48285:intron 30.0%
!!! ACCTCCATTTGATTTTTTGT+TGG + chr8.1:20316904-20316923 MS.gene48285:CDS 30.0%
!!! CTTTTTTGGTTTGGTGAAAT+TGG + chr8.1:20316146-20316165 MS.gene48285:CDS 30.0%
AACACAGTAAAGTTGTAAGG+AGG - chr8.1:20316752-20316771 None:intergenic 35.0%
ATCTGAGTTTGAATAGACTG+AGG - chr8.1:20316050-20316069 None:intergenic 35.0%
ATGAAGAATCCAAGCAAGTA+TGG + chr8.1:20316686-20316705 MS.gene48285:CDS 35.0%
CAGAATATCTGTTTGTGGAA+TGG - chr8.1:20315854-20315873 None:intergenic 35.0%
CAGTCTCAGAAGATACTATA+AGG + chr8.1:20316201-20316220 MS.gene48285:CDS 35.0%
CATAAACTATGACTGCATGT+GGG - chr8.1:20316637-20316656 None:intergenic 35.0%
CATATGGTGAGTTTGATACA+TGG - chr8.1:20315815-20315834 None:intergenic 35.0%
CATGTATCAAACTCACCATA+TGG + chr8.1:20315813-20315832 MS.gene48285:CDS 35.0%
GCAAACACAGTAAAGTTGTA+AGG - chr8.1:20316755-20316774 None:intergenic 35.0%
GCCCTTCTCAGTATATTAAT+TGG + chr8.1:20316810-20316829 MS.gene48285:CDS 35.0%
TCCACAAACAGATATTCTGA+TGG + chr8.1:20315855-20315874 MS.gene48285:CDS 35.0%
TCCATCAGAATATCTGTTTG+TGG - chr8.1:20315859-20315878 None:intergenic 35.0%
TCTTACCGAAGCTATGTATA+AGG + chr8.1:20316844-20316863 MS.gene48285:CDS 35.0%
TCTTTGAATCCATACTTGCT+TGG - chr8.1:20316698-20316717 None:intergenic 35.0%
TTGGAAAACAAAGAGAGACA+TGG + chr8.1:20316923-20316942 MS.gene48285:CDS 35.0%
TTGGATTCTTCATCACAGTA+CGG - chr8.1:20316679-20316698 None:intergenic 35.0%
! GATCTTTTGATGTGACGATA+AGG - chr8.1:20315933-20315952 None:intergenic 35.0%
! TAAGGTTGTGATTGTTGCTT+AGG - chr8.1:20316577-20316596 None:intergenic 35.0%
! TTTACTGTGTTTGCTACTTG+TGG + chr8.1:20316761-20316780 MS.gene48285:CDS 35.0%
!!! GATGAGACTCTTTTTTGGTT+TGG + chr8.1:20316137-20316156 MS.gene48285:CDS 35.0%
AATAAGTACTTGGAGAGCCA+AGG + chr8.1:20316080-20316099 MS.gene48285:CDS 40.0%
AATCAACTCCTTGACAACCT+TGG - chr8.1:20316100-20316119 None:intergenic 40.0%
GCATAAACTATGACTGCATG+TGG - chr8.1:20316638-20316657 None:intergenic 40.0%
TGACGATAAGGAGGAAGATA+AGG - chr8.1:20315921-20315940 None:intergenic 40.0%
TTGCAGTCATTGCTTGAGAA+GGG + chr8.1:20316437-20316456 MS.gene48285:intron 40.0%
TTTGCAGTCATTGCTTGAGA+AGG + chr8.1:20316436-20316455 MS.gene48285:intron 40.0%
! CATTGATAATTCGCGAGGAT+CGG - chr8.1:20316378-20316397 None:intergenic 40.0%
! CTTTTGATGTGACGATAAGG+AGG - chr8.1:20315930-20315949 None:intergenic 40.0%
!! GAAAGCATTGATAATTCGCG+AGG - chr8.1:20316383-20316402 None:intergenic 40.0%
!! GTAATTTGGCTTGCAGCATA+AGG - chr8.1:20316595-20316614 None:intergenic 40.0%
!! TATTTGTTGGTCTTGGACCT+TGG + chr8.1:20316325-20316344 MS.gene48285:intron 40.0%
ACCTTGCACAACTAGGTACT+TGG - chr8.1:20316465-20316484 None:intergenic 45.0%
CTAGTGCAGACAGTAGCATT+AGG - chr8.1:20316791-20316810 None:intergenic 45.0%
GATACATGGCCAAAACCACT+TGG - chr8.1:20315801-20315820 None:intergenic 45.0%
GATATGTTCAGTGTCTGCTG+TGG + chr8.1:20316716-20316735 MS.gene48285:CDS 45.0%
!! GGTGTTAACTTTGCTGTTGC+TGG + chr8.1:20315966-20315985 MS.gene48285:CDS 45.0%
AAGTCGTCTCTGTCATCTGG+AGG - chr8.1:20316530-20316549 None:intergenic 50.0%
CAGTGTCTCAGGAGCTTTAC+AGG + chr8.1:20316238-20316257 MS.gene48285:CDS 50.0%
CCAGATGACAGAGACGACTT+AGG + chr8.1:20316530-20316549 MS.gene48285:CDS 50.0%
CCTAAGTCGTCTCTGTCATC+TGG - chr8.1:20316533-20316552 None:intergenic 50.0%
CTGTAAAGCTCCTGAGACAC+TGG - chr8.1:20316240-20316259 None:intergenic 50.0%
CTTGCAATGTCCAGTGTCTC+AGG + chr8.1:20316227-20316246 MS.gene48285:CDS 50.0%
GCCAAGTACCTAGTTGTGCA+AGG + chr8.1:20316461-20316480 MS.gene48285:CDS 50.0%
GGAGACTCTTTCACAGACAC+AGG + chr8.1:20315753-20315772 MS.gene48285:CDS 50.0%
GTCTGTGAAAGAGTCTCCGA+AGG - chr8.1:20315751-20315770 None:intergenic 50.0%
GTGGATGACCTTGCACAACT+AGG - chr8.1:20316472-20316491 None:intergenic 50.0%
TGCAAGGTCATCCACCAACA+GGG + chr8.1:20316477-20316496 MS.gene48285:CDS 50.0%
TTCAGTGTCTGCTGTGGATC+AGG + chr8.1:20316722-20316741 MS.gene48285:CDS 50.0%
TTGGAGAGCCAAGGTTGTCA+AGG + chr8.1:20316089-20316108 MS.gene48285:CDS 50.0%
! GCTGAAGGTCCAAGTGGTTT+TGG + chr8.1:20315789-20315808 MS.gene48285:CDS 50.0%
CACAACGCTGAAGGTCCAAG+TGG + chr8.1:20315783-20315802 MS.gene48285:CDS 55.0%
GGCAACACACACAACGCTGA+AGG + chr8.1:20315774-20315793 MS.gene48285:CDS 55.0%
GTGCAAGGTCATCCACCAAC+AGG + chr8.1:20316476-20316495 MS.gene48285:CDS 55.0%
! AGTGTAAGGCACCCTGTTGG+TGG - chr8.1:20316491-20316510 None:intergenic 55.0%
! GTCAGTGTAAGGCACCCTGT+TGG - chr8.1:20316494-20316513 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 20315691 20316949 20315691 ID=MS.gene48285
chr8.1 mRNA 20315691 20316949 20315691 ID=MS.gene48285.t1;Parent=MS.gene48285
chr8.1 exon 20315691 20316259 20315691 ID=MS.gene48285.t1.exon1;Parent=MS.gene48285.t1
chr8.1 CDS 20315691 20316259 20315691 ID=cds.MS.gene48285.t1;Parent=MS.gene48285.t1
chr8.1 exon 20316443 20316949 20316443 ID=MS.gene48285.t1.exon2;Parent=MS.gene48285.t1
chr8.1 CDS 20316443 20316949 20316443 ID=cds.MS.gene48285.t1;Parent=MS.gene48285.t1
Gene Sequence

>MS.gene48285

CTTCTTCAGCTACATCAACTACAGAACAACTTTCAAGACCTTTCAAGAAAATTTACGCCTTCGGAGACTCTTTCACAGACACAGGCAACACACACAACGCTGAAGGTCCAAGTGGTTTTGGCCATGTATCAAACTCACCATATGGAACAACTTTCTTCAACCATTCCACAAACAGATATTCTGATGGAAGACTTGTTATTGACTTTGTCACAGAATCACTTTCTTTGCCTTATCTTCCTCCTTATCGTCACATCAAAAGATCAAATGATACTTTTGGTGTTAACTTTGCTGTTGCTGGTTCAACAGCTATAAACCATGAATTCTTTGTTAGGAATAATCTTTCTCTTGACATCACTCCTCAGTCTATTCAAACTCAGATATTGTGGTTTAATAAGTACTTGGAGAGCCAAGGTTGTCAAGGAGTTGATTCAAAGTGTAAAGATTTTGATGAGACTCTTTTTTGGTTTGGTGAAATTGGTGTTAATGATTATGCTTACACTCTTGGATCTACAGTCTCAGAAGATACTATAAGGAAGCTTGCAATGTCCAGTGTCTCAGGAGCTTTACAGGTAATATACTATTTGATTCATGTTTAAGATTAGTGCACATAAAAATGGGAGTTATATATATGGTTTATTTGTTGGTCTTGGACCTTGGTCTAGAGATTTTTTTTCATTCTTACAACCGATCCTCGCGAATTATCAATGCTTTCATATATTAACTGTTTGTTATATTCTTTGTTAATTTTGCAGTCATTGCTTGAGAAGGGTGCCAAGTACCTAGTTGTGCAAGGTCATCCACCAACAGGGTGCCTTACACTGACAATGTATTTAGCTCCTCCAGATGACAGAGACGACTTAGGATGTGTGAAAAGTGCTAACGACCTAAGCAACAATCACAACCTTATGCTGCAAGCCAAATTACAAGAATTCAGGAAACAGTACCCACATGCAGTCATAGTTTATGCTGATTACTTCAATGCATACCGTACTGTGATGAAGAATCCAAGCAAGTATGGATTCAAAGATATGTTCAGTGTCTGCTGTGGATCAGGAGAACCTCCTTACAACTTTACTGTGTTTGCTACTTGTGGTACACCTAATGCTACTGTCTGCACTAGCCCTTCTCAGTATATTAATTGGGATGGTGTTCATCTTACCGAAGCTATGTATAAGGTTGTTTCTAATATGTTTCTTCAAGGAAATTTCAGCCAACCTCCATTTGATTTTTTGTTGGAAAACAAAGAGAGACATGGCTGA

Protein sequence

>MS.gene48285.t1

LLQLHQLQNNFQDLSRKFTPSETLSQTQATHTTLKVQVVLAMYQTHHMEQLSSTIPQTDILMEDLLLTLSQNHFLCLIFLLIVTSKDQMILLVLTLLLLVQQLUTMNSLLGIIFLLTSLLSLFKLRYCGLISTWRAKVVKELIQSVKILMRLFFGLVKLVLMIMLTLLDLQSQKILUGSLQCPVSQELYSHCLRRVPSTULCKVIHQQGALHUQCIULLQMTETTUDVUKVLTTUATITTLCCKPNYKNSGNSTHMQSUFMLITSMHTVLUURIQASMDSKICSVSAVDQENLLTTLLCLLLVVHLMLLSALALLSILIGMVFILPKLCIRLFLICFFKEISANLHLIFCWKTKRDMA