Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50072.t1 | XP_003606871.2 | 93.9 | 165 | 10 | 0 | 1 | 165 | 137 | 301 | 5.90E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50072.t1 | O65009 | 44.1 | 161 | 85 | 2 | 1 | 156 | 122 | 282 | 2.6e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50072.t1 | G7JG28 | 93.9 | 165 | 10 | 0 | 1 | 165 | 132 | 296 | 4.2e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene50072 | -0.83519 | 1.94E-56 | -1.69E-46 |
| MS.gene04921 | MS.gene50072 | 0.815292 | 9.96E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene50072 | -0.800327 | 1.52E-48 | -1.69E-46 |
| MS.gene04932 | MS.gene50072 | 0.825426 | 4.72E-54 | -1.69E-46 |
| MS.gene049549 | MS.gene50072 | 0.805289 | 1.43E-49 | -1.69E-46 |
| MS.gene049633 | MS.gene50072 | -0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene50072 | -0.837338 | 5.52E-57 | -1.69E-46 |
| MS.gene050323 | MS.gene50072 | -0.820289 | 7.42E-53 | -1.69E-46 |
| MS.gene050338 | MS.gene50072 | 0.849894 | 2.41E-60 | -1.69E-46 |
| MS.gene050444 | MS.gene50072 | -0.813989 | 1.94E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene50072 | -0.804205 | 2.42E-49 | -1.69E-46 |
| MS.gene050577 | MS.gene50072 | -0.839929 | 1.18E-57 | -1.69E-46 |
| MS.gene050612 | MS.gene50072 | -0.814 | 1.92E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene50072 | -0.820034 | 8.49E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene50072 | -0.818241 | 2.17E-52 | -1.69E-46 |
| MS.gene050904 | MS.gene50072 | -0.818155 | 2.27E-52 | -1.69E-46 |
| MS.gene051138 | MS.gene50072 | 0.806394 | 8.40E-50 | -1.69E-46 |
| MS.gene051317 | MS.gene50072 | -0.814852 | 1.25E-51 | -1.69E-46 |
| MS.gene051439 | MS.gene50072 | 0.825689 | 4.09E-54 | -1.69E-46 |
| MS.gene051651 | MS.gene50072 | 0.829619 | 4.65E-55 | -1.69E-46 |
| MS.gene051838 | MS.gene50072 | -0.802762 | 4.81E-49 | -1.69E-46 |
| MS.gene051874 | MS.gene50072 | -0.80067 | 1.29E-48 | -1.69E-46 |
| MS.gene052129 | MS.gene50072 | 0.809205 | 2.12E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene50072 | -0.817754 | 2.80E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene50072 | 0.844747 | 6.24E-59 | -1.69E-46 |
| MS.gene052823 | MS.gene50072 | -0.824725 | 6.91E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene50072 | -0.816085 | 6.63E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene50072 | -0.819216 | 1.30E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene50072 | -0.830343 | 3.10E-55 | -1.69E-46 |
| MS.gene053480 | MS.gene50072 | -0.806855 | 6.72E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene50072 | -0.806915 | 6.52E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene50072 | 0.816729 | 4.76E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene50072 | -0.84924 | 3.67E-60 | -1.69E-46 |
| MS.gene054884 | MS.gene50072 | 0.828567 | 8.37E-55 | -1.69E-46 |
| MS.gene054941 | MS.gene50072 | 0.800712 | 1.27E-48 | -1.69E-46 |
| MS.gene054954 | MS.gene50072 | -0.800762 | 1.24E-48 | -1.69E-46 |
| MS.gene055251 | MS.gene50072 | 0.805399 | 1.36E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene50072 | -0.816029 | 6.83E-52 | -1.69E-46 |
| MS.gene055577 | MS.gene50072 | -0.806738 | 7.11E-50 | -1.69E-46 |
| MS.gene055731 | MS.gene50072 | 0.810971 | 8.84E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene50072 | -0.829341 | 5.43E-55 | -1.69E-46 |
| MS.gene055751 | MS.gene50072 | -0.825612 | 4.26E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene50072 | -0.848499 | 5.90E-60 | -1.69E-46 |
| MS.gene056224 | MS.gene50072 | 0.800849 | 1.19E-48 | -1.69E-46 |
| MS.gene056266 | MS.gene50072 | -0.80048 | 1.41E-48 | -1.69E-46 |
| MS.gene056387 | MS.gene50072 | -0.822026 | 2.95E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene50072 | -0.833736 | 4.49E-56 | -1.69E-46 |
| MS.gene056590 | MS.gene50072 | 0.835456 | 1.66E-56 | -1.69E-46 |
| MS.gene056591 | MS.gene50072 | 0.833313 | 5.73E-56 | -1.69E-46 |
| MS.gene056618 | MS.gene50072 | 0.804513 | 2.08E-49 | -1.69E-46 |
| MS.gene056678 | MS.gene50072 | 0.801058 | 1.08E-48 | -1.69E-46 |
| MS.gene056688 | MS.gene50072 | 0.821298 | 4.35E-53 | -1.69E-46 |
| MS.gene056692 | MS.gene50072 | -0.825416 | 4.74E-54 | -1.69E-46 |
| MS.gene056694 | MS.gene50072 | 0.920334 | 1.46E-87 | -1.69E-46 |
| MS.gene056697 | MS.gene50072 | 0.901599 | 2.28E-78 | -1.69E-46 |
| MS.gene056856 | MS.gene50072 | 0.832449 | 9.39E-56 | -1.69E-46 |
| MS.gene057261 | MS.gene50072 | 0.808031 | 3.78E-50 | -1.69E-46 |
| MS.gene057658 | MS.gene50072 | -0.824376 | 8.35E-54 | -1.69E-46 |
| MS.gene057717 | MS.gene50072 | 0.832821 | 7.59E-56 | -1.69E-46 |
| MS.gene057768 | MS.gene50072 | 0.825197 | 5.34E-54 | -1.69E-46 |
| MS.gene057831 | MS.gene50072 | -0.819333 | 1.23E-52 | -1.69E-46 |
| MS.gene058334 | MS.gene50072 | 0.837069 | 6.46E-57 | -1.69E-46 |
| MS.gene05837 | MS.gene50072 | -0.800169 | 1.64E-48 | -1.69E-46 |
| MS.gene058382 | MS.gene50072 | 0.821316 | 4.31E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene50072 | -0.820986 | 5.13E-53 | -1.69E-46 |
| MS.gene058452 | MS.gene50072 | 0.805551 | 1.26E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene50072 | -0.817297 | 3.55E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene50072 | -0.842016 | 3.34E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene50072 | -0.841421 | 4.80E-58 | -1.69E-46 |
| MS.gene05914 | MS.gene50072 | -0.804279 | 2.33E-49 | -1.69E-46 |
| MS.gene05926 | MS.gene50072 | 0.843113 | 1.71E-58 | -1.69E-46 |
| MS.gene05960 | MS.gene50072 | 0.807757 | 4.33E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene50072 | -0.809438 | 1.89E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene50072 | -0.81107 | 8.42E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene50072 | -0.837054 | 6.52E-57 | -1.69E-46 |
| MS.gene060087 | MS.gene50072 | 0.812256 | 4.65E-51 | -1.69E-46 |
| MS.gene06027 | MS.gene50072 | -0.813258 | 2.80E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene50072 | 0.819587 | 1.07E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene50072 | -0.819469 | 1.14E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene50072 | -0.81367 | 2.28E-51 | -1.69E-46 |
| MS.gene060823 | MS.gene50072 | 0.807119 | 5.91E-50 | -1.69E-46 |
| MS.gene06084 | MS.gene50072 | 0.819682 | 1.02E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene50072 | -0.835416 | 1.70E-56 | -1.69E-46 |
| MS.gene061044 | MS.gene50072 | 0.879761 | 9.57E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50072.t1 | MTR_4g069680 | 93.939 | 165 | 10 | 0 | 1 | 165 | 132 | 296 | 8.58e-118 | 335 |
| MS.gene50072.t1 | MTR_3g116410 | 75.758 | 165 | 40 | 0 | 1 | 165 | 121 | 285 | 1.09e-92 | 271 |
| MS.gene50072.t1 | MTR_3g107830 | 72.121 | 165 | 46 | 0 | 1 | 165 | 101 | 265 | 5.55e-88 | 258 |
| MS.gene50072.t1 | MTR_3g116380 | 53.548 | 155 | 71 | 1 | 1 | 155 | 149 | 302 | 2.70e-60 | 189 |
| MS.gene50072.t1 | MTR_3g116270 | 47.742 | 155 | 81 | 0 | 1 | 155 | 351 | 505 | 5.95e-50 | 167 |
| MS.gene50072.t1 | MTR_4g069570 | 45.223 | 157 | 84 | 2 | 1 | 155 | 179 | 335 | 1.81e-46 | 154 |
| MS.gene50072.t1 | MTR_3g116320 | 45.806 | 155 | 84 | 0 | 1 | 155 | 533 | 687 | 4.65e-46 | 159 |
| MS.gene50072.t1 | MTR_4g069550 | 46.497 | 157 | 82 | 2 | 1 | 155 | 306 | 462 | 1.11e-45 | 155 |
| MS.gene50072.t1 | MTR_4g069040 | 43.293 | 164 | 91 | 2 | 1 | 162 | 353 | 516 | 7.00e-45 | 154 |
| MS.gene50072.t1 | MTR_3g109490 | 45.912 | 159 | 80 | 4 | 1 | 156 | 167 | 322 | 4.02e-44 | 148 |
| MS.gene50072.t1 | MTR_1g008500 | 47.742 | 155 | 76 | 4 | 3 | 156 | 235 | 385 | 8.54e-44 | 149 |
| MS.gene50072.t1 | MTR_1g008530 | 46.104 | 154 | 78 | 4 | 4 | 156 | 75 | 224 | 1.36e-43 | 144 |
| MS.gene50072.t1 | MTR_6g086530 | 44.025 | 159 | 86 | 3 | 1 | 156 | 48 | 206 | 2.27e-42 | 140 |
| MS.gene50072.t1 | MTR_1g008580 | 45.395 | 152 | 78 | 4 | 4 | 154 | 23 | 170 | 3.42e-42 | 139 |
| MS.gene50072.t1 | MTR_4g069520 | 43.949 | 157 | 86 | 2 | 1 | 155 | 566 | 722 | 7.41e-42 | 148 |
| MS.gene50072.t1 | MTR_4g069520 | 43.949 | 157 | 86 | 2 | 1 | 155 | 552 | 708 | 8.35e-42 | 148 |
| MS.gene50072.t1 | MTR_0312s0030 | 45.806 | 155 | 78 | 3 | 3 | 156 | 176 | 325 | 2.13e-41 | 141 |
| MS.gene50072.t1 | MTR_1g007520 | 45.806 | 155 | 78 | 3 | 3 | 156 | 176 | 325 | 2.13e-41 | 141 |
| MS.gene50072.t1 | MTR_1g008590 | 45.806 | 155 | 78 | 3 | 3 | 156 | 176 | 325 | 2.37e-41 | 141 |
| MS.gene50072.t1 | MTR_1g008560 | 45.455 | 154 | 79 | 4 | 4 | 156 | 181 | 330 | 7.70e-41 | 140 |
| MS.gene50072.t1 | MTR_1g008510 | 45.455 | 154 | 79 | 4 | 4 | 156 | 177 | 326 | 8.02e-41 | 139 |
| MS.gene50072.t1 | MTR_1g008450 | 42.857 | 154 | 83 | 3 | 3 | 156 | 165 | 313 | 1.04e-37 | 131 |
| MS.gene50072.t1 | MTR_1g008470 | 40.909 | 154 | 86 | 3 | 3 | 156 | 152 | 300 | 5.31e-37 | 129 |
| MS.gene50072.t1 | MTR_1g008460 | 41.611 | 149 | 82 | 3 | 8 | 156 | 154 | 297 | 3.92e-34 | 122 |
| MS.gene50072.t1 | MTR_4g068420 | 42.446 | 139 | 59 | 3 | 19 | 155 | 1 | 120 | 3.56e-33 | 114 |
| MS.gene50072.t1 | MTR_8g064500 | 33.962 | 159 | 93 | 5 | 1 | 154 | 220 | 371 | 2.93e-25 | 99.8 |
| MS.gene50072.t1 | MTR_3g078090 | 33.548 | 155 | 97 | 3 | 1 | 153 | 474 | 624 | 1.55e-22 | 93.6 |
| MS.gene50072.t1 | MTR_8g064560 | 33.333 | 159 | 94 | 6 | 1 | 154 | 473 | 624 | 1.74e-22 | 93.2 |
| MS.gene50072.t1 | MTR_8g064530 | 32.911 | 158 | 94 | 5 | 1 | 153 | 490 | 640 | 2.57e-22 | 92.8 |
| MS.gene50072.t1 | MTR_5g034320 | 32.704 | 159 | 95 | 5 | 1 | 154 | 476 | 627 | 3.20e-22 | 92.4 |
| MS.gene50072.t1 | MTR_4g133840 | 31.818 | 154 | 100 | 3 | 3 | 154 | 37 | 187 | 4.11e-22 | 88.2 |
| MS.gene50072.t1 | MTR_2g081610 | 33.333 | 144 | 86 | 5 | 14 | 157 | 128 | 261 | 4.42e-18 | 79.0 |
| MS.gene50072.t1 | MTR_8g046000 | 35.135 | 148 | 86 | 6 | 8 | 155 | 85 | 222 | 1.47e-17 | 77.0 |
| MS.gene50072.t1 | MTR_2g081590 | 32.215 | 149 | 91 | 5 | 9 | 157 | 123 | 261 | 7.16e-17 | 75.9 |
| MS.gene50072.t1 | MTR_8g445140 | 34.043 | 141 | 83 | 5 | 15 | 155 | 172 | 302 | 2.23e-16 | 74.7 |
| MS.gene50072.t1 | MTR_8g445170 | 34.043 | 141 | 83 | 5 | 15 | 155 | 253 | 383 | 3.22e-16 | 75.1 |
| MS.gene50072.t1 | MTR_5g033350 | 29.221 | 154 | 104 | 4 | 1 | 153 | 49 | 198 | 3.51e-16 | 72.8 |
| MS.gene50072.t1 | MTR_8g045890 | 35.172 | 145 | 84 | 6 | 15 | 159 | 301 | 435 | 1.75e-15 | 73.2 |
| MS.gene50072.t1 | MTR_5g033340 | 29.221 | 154 | 104 | 4 | 1 | 153 | 228 | 377 | 1.77e-15 | 72.8 |
| MS.gene50072.t1 | MTR_5g033340 | 28.986 | 138 | 93 | 4 | 1 | 137 | 20 | 153 | 8.00e-11 | 59.7 |
| MS.gene50072.t1 | MTR_8g045890 | 35.172 | 145 | 84 | 6 | 15 | 159 | 379 | 513 | 1.88e-15 | 73.2 |
| MS.gene50072.t1 | MTR_8g045890 | 35.172 | 145 | 84 | 6 | 15 | 159 | 342 | 476 | 2.11e-15 | 72.8 |
| MS.gene50072.t1 | MTR_1g045860 | 31.126 | 151 | 89 | 5 | 8 | 155 | 121 | 259 | 2.58e-14 | 68.9 |
| MS.gene50072.t1 | MTR_8g045880 | 35.461 | 141 | 81 | 5 | 15 | 155 | 184 | 314 | 2.99e-14 | 69.3 |
| MS.gene50072.t1 | MTR_8g045880 | 35.461 | 141 | 81 | 5 | 15 | 155 | 219 | 349 | 4.28e-14 | 68.9 |
| MS.gene50072.t1 | MTR_8g045880 | 35.461 | 141 | 81 | 5 | 15 | 155 | 221 | 351 | 4.66e-14 | 68.9 |
| MS.gene50072.t1 | MTR_5g033330 | 28.571 | 154 | 105 | 4 | 1 | 153 | 467 | 616 | 8.58e-14 | 68.6 |
| MS.gene50072.t1 | MTR_5g033330 | 28.986 | 138 | 93 | 4 | 1 | 137 | 51 | 184 | 1.80e-11 | 61.6 |
| MS.gene50072.t1 | MTR_5g033330 | 28.986 | 138 | 93 | 4 | 1 | 137 | 259 | 392 | 4.65e-11 | 60.5 |
| MS.gene50072.t1 | MTR_8g075930 | 38.667 | 75 | 43 | 2 | 72 | 144 | 14 | 87 | 1.82e-13 | 62.8 |
| MS.gene50072.t1 | MTR_8g044290 | 36.975 | 119 | 64 | 6 | 8 | 126 | 229 | 336 | 9.52e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50072.t1 | AT1G49320 | 44.654 | 159 | 83 | 2 | 3 | 156 | 122 | 280 | 9.27e-45 | 149 |
| MS.gene50072.t1 | AT5G25610 | 42.581 | 155 | 83 | 4 | 1 | 153 | 239 | 389 | 1.38e-35 | 127 |
| MS.gene50072.t1 | AT1G70370 | 35.443 | 158 | 90 | 4 | 1 | 153 | 472 | 622 | 1.87e-24 | 99.0 |
| MS.gene50072.t1 | AT1G70370 | 35.443 | 158 | 90 | 4 | 1 | 153 | 472 | 622 | 1.87e-24 | 99.0 |
| MS.gene50072.t1 | AT1G23760 | 33.548 | 155 | 97 | 3 | 1 | 153 | 468 | 618 | 8.13e-23 | 94.4 |
| MS.gene50072.t1 | AT1G60390 | 31.169 | 154 | 94 | 5 | 5 | 153 | 474 | 620 | 6.93e-18 | 80.1 |
Find 41 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATTTGGGTTCCAAAAT+TGG | 0.164811 | 4.2:+37075451 | MS.gene50072:CDS |
| CATGTTTCATTTCAGGTTCT+TGG | 0.168453 | 4.2:+37075380 | MS.gene50072:CDS |
| ACACCCTTAACTTCCAATTT+TGG | 0.169578 | 4.2:-37075464 | None:intergenic |
| ACATGGTCATGTTTCATTTC+AGG | 0.187970 | 4.2:+37075373 | MS.gene50072:CDS |
| GATTTCACTCAAACCATTCT+TGG | 0.227601 | 4.2:+37075086 | MS.gene50072:CDS |
| CAATTTCACTTCTCCTTTGA+TGG | 0.228547 | 4.2:-37075036 | None:intergenic |
| GTTTCACTTGTTGGTGATAA+TGG | 0.231201 | 4.2:+37075299 | MS.gene50072:CDS |
| TTCCAGCTGATAATTTCATT+TGG | 0.279557 | 4.2:+37075438 | MS.gene50072:CDS |
| TTTCACTTGTTGGTGATAAT+GGG | 0.318089 | 4.2:+37075300 | MS.gene50072:CDS |
| CTGAAAAGTGACACTTGATT+TGG | 0.319135 | 4.2:-37075150 | None:intergenic |
| TGTTGGTGATAATGGGGATT+TGG | 0.320872 | 4.2:+37075307 | MS.gene50072:CDS |
| TCCAGCTGATAATTTCATTT+GGG | 0.338146 | 4.2:+37075439 | MS.gene50072:CDS |
| GTGTGACATGCCACCATCTT+TGG | 0.354478 | 4.2:-37075212 | None:intergenic |
| TCATATGGATACTTCTCATT+GGG | 0.365983 | 4.2:+37075349 | MS.gene50072:CDS |
| GGTTCCAAAATTGGAAGTTA+AGG | 0.373261 | 4.2:+37075460 | MS.gene50072:CDS |
| GTTCCAAAATTGGAAGTTAA+GGG | 0.383479 | 4.2:+37075461 | MS.gene50072:CDS |
| CAGGTTCTTGGTGTTGTGCC+AGG | 0.389898 | 4.2:+37075392 | MS.gene50072:CDS |
| TGACAATAGAAAACAGCATA+AGG | 0.411222 | 4.2:-37075245 | None:intergenic |
| ATATATAGAGTTTCACTTGT+TGG | 0.415560 | 4.2:+37075290 | MS.gene50072:CDS |
| GTCATATGGATACTTCTCAT+TGG | 0.421442 | 4.2:+37075348 | MS.gene50072:CDS |
| AGCTATGGTTGTTTGTCATA+TGG | 0.433565 | 4.2:+37075334 | MS.gene50072:CDS |
| GTTTGCTCTCACATTCTGTA+AGG | 0.443520 | 4.2:-37075013 | None:intergenic |
| CTATTTCAGACAAGATTATG+AGG | 0.450606 | 4.2:+37075180 | MS.gene50072:CDS |
| TGGGGATTTGGTGGAAGCTA+TGG | 0.459502 | 4.2:+37075319 | MS.gene50072:CDS |
| TGACACACATTAGAACTTCC+TGG | 0.463418 | 4.2:-37075410 | None:intergenic |
| TGGTGATAATGGGGATTTGG+TGG | 0.472374 | 4.2:+37075310 | MS.gene50072:CDS |
| AATTTCACTTCTCCTTTGAT+GGG | 0.484291 | 4.2:-37075035 | None:intergenic |
| GACAATAGAAAACAGCATAA+GGG | 0.499892 | 4.2:-37075244 | None:intergenic |
| ATATCCTATTCTCACTGTCT+TGG | 0.512574 | 4.2:-37075271 | None:intergenic |
| AAGCATAGATTCTAAAGAAG+TGG | 0.527429 | 4.2:-37075063 | None:intergenic |
| ACCCAAATGAAATTATCAGC+TGG | 0.527506 | 4.2:-37075440 | None:intergenic |
| GAGGATTTCAGCTCCAAAGA+TGG | 0.529926 | 4.2:+37075199 | MS.gene50072:CDS |
| ATAGCCAAGACAGTGAGAAT+AGG | 0.536729 | 4.2:+37075267 | MS.gene50072:CDS |
| TTTGCTCTCACATTCTGTAA+GGG | 0.540182 | 4.2:-37075012 | None:intergenic |
| TAGAAAACAGCATAAGGGTA+AGG | 0.540843 | 4.2:-37075239 | None:intergenic |
| TTCACTTGTTGGTGATAATG+GGG | 0.549004 | 4.2:+37075301 | MS.gene50072:CDS |
| GATACTTCTCATTGGGGACA+TGG | 0.551200 | 4.2:+37075356 | MS.gene50072:CDS |
| TGTGAGAGCAAACCCATCAA+AGG | 0.590120 | 4.2:+37075023 | MS.gene50072:CDS |
| CATATGGATACTTCTCATTG+GGG | 0.635326 | 4.2:+37075350 | MS.gene50072:CDS |
| TTCTGTAAGGGTATCTACCA+TGG | 0.635870 | 4.2:-37075000 | None:intergenic |
| GATTTCAGCTCCAAAGATGG+TGG | 0.658692 | 4.2:+37075202 | MS.gene50072:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATATAGAGTTTCACTTGT+TGG | + | chr4.2:37075290-37075309 | MS.gene50072:CDS | 25.0% |
| ! | ATCATTTGAAAAACCAAGAA+TGG | - | chr4.2:37075102-37075121 | None:intergenic | 25.0% |
| AATTTCACTTCTCCTTTGAT+GGG | - | chr4.2:37075038-37075057 | None:intergenic | 30.0% | |
| CTATTTCAGACAAGATTATG+AGG | + | chr4.2:37075180-37075199 | MS.gene50072:CDS | 30.0% | |
| GACAATAGAAAACAGCATAA+GGG | - | chr4.2:37075247-37075266 | None:intergenic | 30.0% | |
| GTTCCAAAATTGGAAGTTAA+GGG | + | chr4.2:37075461-37075480 | MS.gene50072:CDS | 30.0% | |
| TCATATGGATACTTCTCATT+GGG | + | chr4.2:37075349-37075368 | MS.gene50072:CDS | 30.0% | |
| TCCAGCTGATAATTTCATTT+GGG | + | chr4.2:37075439-37075458 | MS.gene50072:CDS | 30.0% | |
| TGACAATAGAAAACAGCATA+AGG | - | chr4.2:37075248-37075267 | None:intergenic | 30.0% | |
| TTCCAGCTGATAATTTCATT+TGG | + | chr4.2:37075438-37075457 | MS.gene50072:CDS | 30.0% | |
| ! | AAGCATAGATTCTAAAGAAG+TGG | - | chr4.2:37075066-37075085 | None:intergenic | 30.0% |
| ! | TTTCATTTGGGTTCCAAAAT+TGG | + | chr4.2:37075451-37075470 | MS.gene50072:CDS | 30.0% |
| !! | TTTCACTTGTTGGTGATAAT+GGG | + | chr4.2:37075300-37075319 | MS.gene50072:CDS | 30.0% |
| ACATGGTCATGTTTCATTTC+AGG | + | chr4.2:37075373-37075392 | MS.gene50072:CDS | 35.0% | |
| ACCCAAATGAAATTATCAGC+TGG | - | chr4.2:37075443-37075462 | None:intergenic | 35.0% | |
| AGCTATGGTTGTTTGTCATA+TGG | + | chr4.2:37075334-37075353 | MS.gene50072:CDS | 35.0% | |
| ATATCCTATTCTCACTGTCT+TGG | - | chr4.2:37075274-37075293 | None:intergenic | 35.0% | |
| CAATTTCACTTCTCCTTTGA+TGG | - | chr4.2:37075039-37075058 | None:intergenic | 35.0% | |
| CATATGGATACTTCTCATTG+GGG | + | chr4.2:37075350-37075369 | MS.gene50072:CDS | 35.0% | |
| CTGAAAAGTGACACTTGATT+TGG | - | chr4.2:37075153-37075172 | None:intergenic | 35.0% | |
| GATTTCACTCAAACCATTCT+TGG | + | chr4.2:37075086-37075105 | MS.gene50072:CDS | 35.0% | |
| GGTTCCAAAATTGGAAGTTA+AGG | + | chr4.2:37075460-37075479 | MS.gene50072:CDS | 35.0% | |
| GTCATATGGATACTTCTCAT+TGG | + | chr4.2:37075348-37075367 | MS.gene50072:CDS | 35.0% | |
| TAGAAAACAGCATAAGGGTA+AGG | - | chr4.2:37075242-37075261 | None:intergenic | 35.0% | |
| TTTGCTCTCACATTCTGTAA+GGG | - | chr4.2:37075015-37075034 | None:intergenic | 35.0% | |
| ! | ACACCCTTAACTTCCAATTT+TGG | - | chr4.2:37075467-37075486 | None:intergenic | 35.0% |
| !! | CATGTTTCATTTCAGGTTCT+TGG | + | chr4.2:37075380-37075399 | MS.gene50072:CDS | 35.0% |
| !! | GTTTCACTTGTTGGTGATAA+TGG | + | chr4.2:37075299-37075318 | MS.gene50072:CDS | 35.0% |
| !! | TTCACTTGTTGGTGATAATG+GGG | + | chr4.2:37075301-37075320 | MS.gene50072:CDS | 35.0% |
| ATAGCCAAGACAGTGAGAAT+AGG | + | chr4.2:37075267-37075286 | MS.gene50072:CDS | 40.0% | |
| GTTTGCTCTCACATTCTGTA+AGG | - | chr4.2:37075016-37075035 | None:intergenic | 40.0% | |
| TGACACACATTAGAACTTCC+TGG | - | chr4.2:37075413-37075432 | None:intergenic | 40.0% | |
| !! | TGTTGGTGATAATGGGGATT+TGG | + | chr4.2:37075307-37075326 | MS.gene50072:CDS | 40.0% |
| GAGGATTTCAGCTCCAAAGA+TGG | + | chr4.2:37075199-37075218 | MS.gene50072:CDS | 45.0% | |
| GATACTTCTCATTGGGGACA+TGG | + | chr4.2:37075356-37075375 | MS.gene50072:CDS | 45.0% | |
| GATTTCAGCTCCAAAGATGG+TGG | + | chr4.2:37075202-37075221 | MS.gene50072:CDS | 45.0% | |
| TGTGAGAGCAAACCCATCAA+AGG | + | chr4.2:37075023-37075042 | MS.gene50072:CDS | 45.0% | |
| !! | TGGTGATAATGGGGATTTGG+TGG | + | chr4.2:37075310-37075329 | MS.gene50072:CDS | 45.0% |
| GTGTGACATGCCACCATCTT+TGG | - | chr4.2:37075215-37075234 | None:intergenic | 50.0% | |
| !! | TGGGGATTTGGTGGAAGCTA+TGG | + | chr4.2:37075319-37075338 | MS.gene50072:CDS | 50.0% |
| !! | CAGGTTCTTGGTGTTGTGCC+AGG | + | chr4.2:37075392-37075411 | MS.gene50072:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 37075002 | 37075499 | 37075002 | ID=MS.gene50072 |
| chr4.2 | mRNA | 37075002 | 37075499 | 37075002 | ID=MS.gene50072.t1;Parent=MS.gene50072 |
| chr4.2 | exon | 37075002 | 37075499 | 37075002 | ID=MS.gene50072.t1.exon1;Parent=MS.gene50072.t1 |
| chr4.2 | CDS | 37075002 | 37075499 | 37075002 | ID=cds.MS.gene50072.t1;Parent=MS.gene50072.t1 |
| Gene Sequence |
| Protein sequence |
