Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55820.t1 | GAU11846.1 | 81.6 | 370 | 57 | 8 | 1 | 365 | 1 | 364 | 5.20E-120 | 441 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55820.t1 | Q9FLE8 | 39.2 | 166 | 88 | 2 | 212 | 364 | 223 | 388 | 1.2e-24 | 115.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55820.t1 | A0A072U5Y7 | 92.2 | 295 | 22 | 1 | 1 | 294 | 1 | 295 | 7.1e-119 | 436.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene55820 | 0.811953 | 5.41E-51 | -1.69E-46 |
| MS.gene049356 | MS.gene55820 | 0.853486 | 2.32E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene55820 | 0.87082 | 1.09E-66 | -1.69E-46 |
| MS.gene049396 | MS.gene55820 | 0.899401 | 2.06E-77 | -1.69E-46 |
| MS.gene049488 | MS.gene55820 | 0.814108 | 1.82E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene55820 | 0.800234 | 1.59E-48 | -1.69E-46 |
| MS.gene049757 | MS.gene55820 | 0.807733 | 4.38E-50 | -1.69E-46 |
| MS.gene050499 | MS.gene55820 | 0.846525 | 2.06E-59 | -1.69E-46 |
| MS.gene050502 | MS.gene55820 | 0.854477 | 1.20E-61 | -1.69E-46 |
| MS.gene050520 | MS.gene55820 | 0.813351 | 2.67E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene55820 | 0.803569 | 3.27E-49 | -1.69E-46 |
| MS.gene050662 | MS.gene55820 | 0.802476 | 5.51E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene55820 | 0.804183 | 2.44E-49 | -1.69E-46 |
| MS.gene050686 | MS.gene55820 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene050697 | MS.gene55820 | 0.805732 | 1.16E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene55820 | 0.812287 | 4.57E-51 | -1.69E-46 |
| MS.gene05080 | MS.gene55820 | 0.809282 | 2.04E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene55820 | 0.808262 | 3.38E-50 | -1.69E-46 |
| MS.gene051509 | MS.gene55820 | 0.886875 | 2.34E-72 | -1.69E-46 |
| MS.gene051874 | MS.gene55820 | 0.835798 | 1.36E-56 | -1.69E-46 |
| MS.gene051941 | MS.gene55820 | 0.825437 | 4.69E-54 | -1.69E-46 |
| MS.gene052346 | MS.gene55820 | 0.826879 | 2.13E-54 | -1.69E-46 |
| MS.gene052414 | MS.gene55820 | 0.834954 | 2.22E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene55820 | 0.803088 | 4.12E-49 | -1.69E-46 |
| MS.gene052999 | MS.gene55820 | 0.812338 | 4.46E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene55820 | 0.805854 | 1.09E-49 | -1.69E-46 |
| MS.gene053449 | MS.gene55820 | 0.851079 | 1.12E-60 | -1.69E-46 |
| MS.gene053505 | MS.gene55820 | 0.832454 | 9.37E-56 | -1.69E-46 |
| MS.gene053574 | MS.gene55820 | 0.84112 | 5.76E-58 | -1.69E-46 |
| MS.gene053626 | MS.gene55820 | 0.830972 | 2.17E-55 | -1.69E-46 |
| MS.gene053758 | MS.gene55820 | 0.818261 | 2.15E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene55820 | 0.820333 | 7.25E-53 | -1.69E-46 |
| MS.gene053889 | MS.gene55820 | 0.807677 | 4.50E-50 | -1.69E-46 |
| MS.gene053912 | MS.gene55820 | 0.905656 | 3.41E-80 | -1.69E-46 |
| MS.gene053916 | MS.gene55820 | 0.828945 | 6.78E-55 | -1.69E-46 |
| MS.gene054486 | MS.gene55820 | 0.826168 | 3.15E-54 | -1.69E-46 |
| MS.gene05494 | MS.gene55820 | 0.829714 | 4.41E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene55820 | 0.818479 | 1.92E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene55820 | 0.808987 | 2.36E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene55820 | 0.84861 | 5.49E-60 | -1.69E-46 |
| MS.gene055744 | MS.gene55820 | 0.847715 | 9.71E-60 | -1.69E-46 |
| MS.gene055909 | MS.gene55820 | 0.864108 | 1.54E-64 | -1.69E-46 |
| MS.gene055911 | MS.gene55820 | 0.862371 | 5.31E-64 | -1.69E-46 |
| MS.gene056166 | MS.gene55820 | 0.83425 | 3.34E-56 | -1.69E-46 |
| MS.gene056387 | MS.gene55820 | 0.8151 | 1.10E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene55820 | 0.870497 | 1.40E-66 | -1.69E-46 |
| MS.gene056511 | MS.gene55820 | 0.856766 | 2.58E-62 | -1.69E-46 |
| MS.gene05664 | MS.gene55820 | 0.838592 | 2.62E-57 | -1.69E-46 |
| MS.gene05666 | MS.gene55820 | 0.847367 | 1.21E-59 | -1.69E-46 |
| MS.gene057019 | MS.gene55820 | 0.827018 | 1.97E-54 | -1.69E-46 |
| MS.gene057076 | MS.gene55820 | 0.852668 | 3.97E-61 | -1.69E-46 |
| MS.gene057086 | MS.gene55820 | 0.856267 | 3.61E-62 | -1.69E-46 |
| MS.gene057537 | MS.gene55820 | 0.844256 | 8.46E-59 | -1.69E-46 |
| MS.gene057734 | MS.gene55820 | 0.889318 | 2.70E-73 | -1.69E-46 |
| MS.gene057876 | MS.gene55820 | 0.816718 | 4.79E-52 | -1.69E-46 |
| MS.gene058075 | MS.gene55820 | 0.833089 | 6.52E-56 | -1.69E-46 |
| MS.gene058120 | MS.gene55820 | 0.852091 | 5.79E-61 | -1.69E-46 |
| MS.gene058130 | MS.gene55820 | 0.814767 | 1.30E-51 | -1.69E-46 |
| MS.gene058459 | MS.gene55820 | 0.867258 | 1.56E-65 | -1.69E-46 |
| MS.gene058928 | MS.gene55820 | 0.830386 | 3.02E-55 | -1.69E-46 |
| MS.gene058948 | MS.gene55820 | 0.821717 | 3.48E-53 | -1.69E-46 |
| MS.gene059129 | MS.gene55820 | 0.82301 | 1.74E-53 | -1.69E-46 |
| MS.gene059305 | MS.gene55820 | 0.805556 | 1.26E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene55820 | 0.820973 | 5.17E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene55820 | 0.843898 | 1.06E-58 | -1.69E-46 |
| MS.gene059368 | MS.gene55820 | 0.862799 | 3.92E-64 | -1.69E-46 |
| MS.gene059402 | MS.gene55820 | -0.809516 | 1.82E-50 | -1.69E-46 |
| MS.gene059455 | MS.gene55820 | 0.81112 | 8.21E-51 | -1.69E-46 |
| MS.gene059517 | MS.gene55820 | 0.80839 | 3.17E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene55820 | 0.862582 | 4.58E-64 | -1.69E-46 |
| MS.gene059680 | MS.gene55820 | 0.822573 | 2.20E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene55820 | 0.850812 | 1.33E-60 | -1.69E-46 |
| MS.gene059807 | MS.gene55820 | 0.86041 | 2.11E-63 | -1.69E-46 |
| MS.gene059865 | MS.gene55820 | 0.812482 | 4.15E-51 | -1.69E-46 |
| MS.gene060372 | MS.gene55820 | 0.876228 | 1.65E-68 | -1.69E-46 |
| MS.gene060482 | MS.gene55820 | 0.815003 | 1.15E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene55820 | 0.827951 | 1.18E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene55820 | 0.831161 | 1.95E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene55820 | 0.845009 | 5.30E-59 | -1.69E-46 |
| MS.gene06097 | MS.gene55820 | 0.895185 | 1.22E-75 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55820.t1 | MTR_8g099665 | 96.739 | 368 | 9 | 2 | 1 | 365 | 1 | 368 | 0.0 | 717 |
| MS.gene55820.t1 | MTR_1g090400 | 45.161 | 186 | 91 | 4 | 180 | 364 | 193 | 368 | 4.76e-46 | 161 |
| MS.gene55820.t1 | MTR_7g104720 | 43.373 | 166 | 83 | 3 | 201 | 364 | 248 | 404 | 3.39e-41 | 149 |
| MS.gene55820.t1 | MTR_7g106500 | 37.719 | 228 | 113 | 6 | 153 | 364 | 64 | 278 | 2.37e-40 | 144 |
| MS.gene55820.t1 | MTR_3g017490 | 44.937 | 158 | 76 | 4 | 210 | 364 | 111 | 260 | 4.47e-36 | 132 |
| MS.gene55820.t1 | MTR_5g083110 | 44.304 | 158 | 79 | 4 | 209 | 365 | 90 | 239 | 6.09e-36 | 131 |
| MS.gene55820.t1 | MTR_8g106900 | 45.181 | 166 | 81 | 3 | 204 | 364 | 113 | 273 | 5.97e-35 | 129 |
| MS.gene55820.t1 | MTR_7g090230 | 39.752 | 161 | 81 | 4 | 210 | 364 | 274 | 424 | 5.07e-32 | 125 |
| MS.gene55820.t1 | MTR_7g010090 | 30.476 | 315 | 156 | 10 | 88 | 364 | 142 | 431 | 9.69e-32 | 124 |
| MS.gene55820.t1 | MTR_8g038440 | 41.176 | 153 | 84 | 3 | 214 | 364 | 106 | 254 | 1.53e-30 | 117 |
| MS.gene55820.t1 | MTR_6g011640 | 37.129 | 202 | 105 | 6 | 180 | 364 | 200 | 396 | 3.49e-30 | 119 |
| MS.gene55820.t1 | MTR_1g051180 | 37.870 | 169 | 85 | 4 | 208 | 364 | 203 | 363 | 2.62e-29 | 116 |
| MS.gene55820.t1 | MTR_5g072400 | 41.139 | 158 | 82 | 4 | 210 | 365 | 106 | 254 | 5.32e-28 | 110 |
| MS.gene55820.t1 | MTR_3g465550 | 39.735 | 151 | 83 | 4 | 210 | 359 | 84 | 227 | 3.78e-27 | 107 |
| MS.gene55820.t1 | MTR_3g071510 | 35.714 | 154 | 90 | 4 | 213 | 365 | 90 | 235 | 6.67e-25 | 102 |
| MS.gene55820.t1 | MTR_3g030520 | 37.267 | 161 | 80 | 5 | 221 | 364 | 142 | 298 | 4.46e-21 | 92.4 |
| MS.gene55820.t1 | MTR_7g079015 | 38.542 | 96 | 51 | 4 | 254 | 345 | 1 | 92 | 8.73e-13 | 64.3 |
| MS.gene55820.t1 | MTR_2g026830 | 38.043 | 92 | 53 | 3 | 254 | 345 | 1 | 88 | 4.59e-12 | 62.4 |
| MS.gene55820.t1 | MTR_1g098230 | 39.286 | 84 | 44 | 3 | 266 | 345 | 12 | 92 | 1.52e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55820.t1 | AT1G64500 | 43.117 | 385 | 180 | 13 | 1 | 365 | 1 | 366 | 8.90e-93 | 282 |
| MS.gene55820.t1 | AT5G06470 | 37.652 | 247 | 108 | 8 | 147 | 364 | 9 | 238 | 1.96e-41 | 145 |
| MS.gene55820.t1 | AT4G10630 | 42.424 | 198 | 101 | 5 | 179 | 364 | 137 | 333 | 3.37e-41 | 147 |
| MS.gene55820.t1 | AT5G03870 | 42.654 | 211 | 103 | 5 | 156 | 364 | 189 | 383 | 4.97e-40 | 145 |
| MS.gene55820.t1 | AT5G39865 | 33.333 | 291 | 156 | 7 | 88 | 364 | 122 | 388 | 1.59e-39 | 144 |
| MS.gene55820.t1 | AT5G01420 | 41.317 | 167 | 90 | 3 | 199 | 364 | 241 | 400 | 5.01e-37 | 138 |
| MS.gene55820.t1 | AT3G28850 | 41.011 | 178 | 80 | 3 | 212 | 364 | 249 | 426 | 7.21e-35 | 133 |
| MS.gene55820.t1 | AT3G57070 | 41.401 | 157 | 85 | 3 | 210 | 364 | 264 | 415 | 1.82e-34 | 131 |
| MS.gene55820.t1 | AT1G32760 | 46.784 | 171 | 84 | 3 | 199 | 364 | 145 | 313 | 3.91e-33 | 125 |
| MS.gene55820.t1 | AT5G13810 | 41.509 | 159 | 80 | 5 | 210 | 364 | 123 | 272 | 5.99e-32 | 121 |
| MS.gene55820.t1 | AT5G58530 | 39.752 | 161 | 87 | 4 | 210 | 364 | 109 | 265 | 1.01e-30 | 118 |
| MS.gene55820.t1 | AT2G41330 | 40.397 | 151 | 82 | 3 | 216 | 364 | 256 | 400 | 3.20e-30 | 119 |
| MS.gene55820.t1 | AT3G11773 | 42.000 | 150 | 77 | 3 | 216 | 364 | 9 | 149 | 1.16e-29 | 112 |
| MS.gene55820.t1 | AT3G11773 | 29.596 | 223 | 111 | 6 | 156 | 364 | 9 | 199 | 3.13e-17 | 79.7 |
Find 109 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTGTGAAGGGTAGGTATT+TGG | 0.146439 | 4.3:-4374173 | MS.gene55820:CDS |
| TCGAGAGAATAGAAAGATTA+AGG | 0.197504 | 4.3:-4374447 | MS.gene55820:CDS |
| CAACAAAGCAAGAGACTTAT+TGG | 0.220866 | 4.3:-4374317 | MS.gene55820:CDS |
| GGGTTAGATTTGTTCCTTGT+TGG | 0.297441 | 4.3:-4374039 | MS.gene55820:CDS |
| GGGTGGGTTGGATGAGTTGA+TGG | 0.303606 | 4.3:-4374152 | MS.gene55820:CDS |
| TTATTATCGGAGGAAGAGTT+AGG | 0.318401 | 4.3:-4374220 | MS.gene55820:CDS |
| GAGACTTATTGGAGAATCAT+AGG | 0.318410 | 4.3:-4374306 | MS.gene55820:CDS |
| TTTGTGAAGGGTAGGTATTT+GGG | 0.321727 | 4.3:-4374172 | MS.gene55820:CDS |
| AGTGAAGGAGTTGTTATTAT+CGG | 0.324310 | 4.3:-4374233 | MS.gene55820:CDS |
| CGTCGGTGGTGGCTGGTATA+CGG | 0.337782 | 4.3:+4374972 | None:intergenic |
| AGTTGTAAGTTGGTTACTGC+TGG | 0.359692 | 4.3:-4374004 | MS.gene55820:CDS |
| TTCTTGGGAGATGGTGTTGT+TGG | 0.375160 | 4.3:+4374888 | None:intergenic |
| ACTACGGCGTCGTTTCCATT+TGG | 0.388509 | 4.3:+4374388 | None:intergenic |
| GGTTAGATTTGTTCCTTGTT+GGG | 0.389547 | 4.3:-4374038 | MS.gene55820:CDS |
| AAGGGTGTGGTGGATGTGGT+GGG | 0.409163 | 4.3:-4374060 | MS.gene55820:CDS |
| CAAGGGTGTGGTGGATGTGG+TGG | 0.419400 | 4.3:-4374061 | MS.gene55820:CDS |
| GGGTTGGTGTGGTCTTGCCA+AGG | 0.424049 | 4.3:-4374198 | MS.gene55820:CDS |
| CTTAATGAAACGGGTCGACT+TGG | 0.425286 | 4.3:-4374127 | MS.gene55820:CDS |
| GAAATAAATTAAAGACAAGT+AGG | 0.431871 | 4.3:+4373932 | None:intergenic |
| GAACAGCGGGTTTGAGTTGT+TGG | 0.436431 | 4.3:+4374776 | None:intergenic |
| GGTGATGGTGGTGTTGGTTG+AGG | 0.438551 | 4.3:+4374745 | None:intergenic |
| AGACAAGGGTGTGGTGGATG+TGG | 0.440591 | 4.3:-4374064 | MS.gene55820:CDS |
| GAGCGTGACGTGGCACTTCA+TGG | 0.441197 | 4.3:-4374271 | MS.gene55820:CDS |
| CTAACTTCATCCCATGATTG+AGG | 0.443754 | 4.3:+4374808 | None:intergenic |
| TTGTGGTGGGAGTTGTAAGT+TGG | 0.448165 | 4.3:-4374014 | MS.gene55820:CDS |
| TTTCTGGATAGTTGCTTAGT+GGG | 0.451141 | 4.3:+4374419 | None:intergenic |
| GGGTAGGTATTTGGGTGGGT+TGG | 0.455353 | 4.3:-4374164 | MS.gene55820:CDS |
| AGAATAGGAGGAGGTAGAGC+AGG | 0.459393 | 4.3:+4374859 | None:intergenic |
| GACGGTGGTGATGGTGGTGT+TGG | 0.459996 | 4.3:+4374739 | None:intergenic |
| ACGGCGTCGTTTCCATTTGG+TGG | 0.460113 | 4.3:+4374391 | None:intergenic |
| GTTGTAAGTTGGTTACTGCT+GGG | 0.466872 | 4.3:-4374003 | MS.gene55820:CDS |
| TTTCAGTAGCTTCGAGGATG+TGG | 0.473934 | 4.3:+4374836 | None:intergenic |
| TTCCTTGTTGGGATTGTGGT+GGG | 0.474378 | 4.3:-4374027 | MS.gene55820:CDS |
| TGCCCCAAGTGTAATGAGAA+TGG | 0.478008 | 4.3:-4373968 | MS.gene55820:CDS |
| CGAAACTCGTCGGTGGTGGC+TGG | 0.479813 | 4.3:+4374965 | None:intergenic |
| GTTGATGGAGCTTAATGAAA+CGG | 0.485172 | 4.3:-4374137 | MS.gene55820:CDS |
| GTTGGTTGAGATTGTGGTTG+TGG | 0.490105 | 4.3:+4374598 | None:intergenic |
| GCTTCGAGGATGTGGAGAAT+AGG | 0.490320 | 4.3:+4374844 | None:intergenic |
| TATTATCGGAGGAAGAGTTA+GGG | 0.490482 | 4.3:-4374219 | MS.gene55820:CDS |
| TGGTGTTGTTGGTGTTGTTG+AGG | 0.493565 | 4.3:+4374899 | None:intergenic |
| GGTGGTGATGACGGTGGTGA+TGG | 0.493874 | 4.3:+4374730 | None:intergenic |
| GCAACCTTACGTGGTGGTTG+TGG | 0.496711 | 4.3:+4374697 | None:intergenic |
| GGTTGGTGTGGTCTTGCCAA+GGG | 0.497158 | 4.3:-4374197 | MS.gene55820:CDS |
| GCCCCAAGTGTAATGAGAAT+GGG | 0.500482 | 4.3:-4373967 | MS.gene55820:CDS |
| GGTTGTGGTTGTTGCGATGG+TGG | 0.504135 | 4.3:+4374712 | None:intergenic |
| TTGATGGAGCTTAATGAAAC+GGG | 0.505824 | 4.3:-4374136 | MS.gene55820:CDS |
| TGAGGAAGTTGTTGAACAGC+GGG | 0.508452 | 4.3:+4374763 | None:intergenic |
| TTCTTCGTTGGTTGAGATTG+TGG | 0.509177 | 4.3:+4374592 | None:intergenic |
| TAACCCATTCTCATTACACT+TGG | 0.509664 | 4.3:+4373964 | None:intergenic |
| TTGCCAAGGGTGTTTGTGAA+GGG | 0.511271 | 4.3:-4374184 | MS.gene55820:CDS |
| TGGCTGGTATACGGCGGGTG+CGG | 0.511794 | 4.3:+4374981 | None:intergenic |
| AACCCATTCTCATTACACTT+GGG | 0.515602 | 4.3:+4373965 | None:intergenic |
| GGTGATGACGGTGGTGATGG+TGG | 0.516672 | 4.3:+4374733 | None:intergenic |
| CTTGCCAAGGGTGTTTGTGA+AGG | 0.518157 | 4.3:-4374185 | MS.gene55820:CDS |
| GTGAAGGGTAGGTATTTGGG+TGG | 0.520089 | 4.3:-4374169 | MS.gene55820:CDS |
| ACCGCGAAACTCGTCGGTGG+TGG | 0.521658 | 4.3:+4374961 | None:intergenic |
| TCGGAGGAAGAGTTAGGGGT+TGG | 0.522640 | 4.3:-4374214 | MS.gene55820:CDS |
| GGTGGTTGTGGTTGTTGCGA+TGG | 0.524805 | 4.3:+4374709 | None:intergenic |
| TGAAGGGTAGGTATTTGGGT+GGG | 0.529660 | 4.3:-4374168 | MS.gene55820:CDS |
| AGGGTGTGGTGGATGTGGTG+GGG | 0.535711 | 4.3:-4374059 | MS.gene55820:CDS |
| AGGATGTGGAGAATAGGAGG+AGG | 0.536843 | 4.3:+4374850 | None:intergenic |
| GCCACCACCGACGAGTTTCG+CGG | 0.538659 | 4.3:-4374962 | MS.gene55820:CDS |
| ACGAAGAACGTTGTTGATGT+TGG | 0.540254 | 4.3:-4374577 | MS.gene55820:CDS |
| AGACATACTAGAGAAACAAA+AGG | 0.541873 | 4.3:-4374488 | MS.gene55820:CDS |
| TTGTAAGTTGGTTACTGCTG+GGG | 0.542030 | 4.3:-4374002 | MS.gene55820:CDS |
| GTTGGAAGACAAGGGTGTGG+TGG | 0.542555 | 4.3:-4374070 | MS.gene55820:CDS |
| GGTGGTGGCTGGTATACGGC+GGG | 0.546279 | 4.3:+4374976 | None:intergenic |
| ACCCATTCTCATTACACTTG+GGG | 0.546633 | 4.3:+4373966 | None:intergenic |
| AGACTTATTGGAGAATCATA+GGG | 0.547504 | 4.3:-4374305 | MS.gene55820:CDS |
| ACATCAACAACGTTCTTCGT+TGG | 0.551192 | 4.3:+4374580 | None:intergenic |
| TTTGTTCCTTGTTGGGATTG+TGG | 0.552057 | 4.3:-4374031 | MS.gene55820:CDS |
| CTACCCTTCACAAACACCCT+TGG | 0.555761 | 4.3:+4374181 | None:intergenic |
| ACAACCACAACCACCACGTA+AGG | 0.559602 | 4.3:-4374701 | MS.gene55820:CDS |
| TAACTCTTCGAGTGTGTGAA+AGG | 0.560614 | 4.3:+4374672 | None:intergenic |
| ATTAAAGACAAGTAGGGCAA+TGG | 0.560759 | 4.3:+4373939 | None:intergenic |
| TCGAGGATGTGGAGAATAGG+AGG | 0.560778 | 4.3:+4374847 | None:intergenic |
| GAGAGAGGTGTTGGAAGACA+AGG | 0.562632 | 4.3:-4374079 | MS.gene55820:CDS |
| ACACGTGTGGAGAGAGGTGT+TGG | 0.566087 | 4.3:-4374088 | MS.gene55820:CDS |
| CAAGGGTGTTTGTGAAGGGT+AGG | 0.566480 | 4.3:-4374180 | MS.gene55820:CDS |
| TTAATGAAACGGGTCGACTT+GGG | 0.568465 | 4.3:-4374126 | MS.gene55820:CDS |
| TTTGTCATCATCAAAACTGA+AGG | 0.573178 | 4.3:-4374530 | MS.gene55820:CDS |
| TGTTGCGATGGTGGTGATGA+CGG | 0.575646 | 4.3:+4374721 | None:intergenic |
| AAATAAATTAAAGACAAGTA+GGG | 0.576677 | 4.3:+4373933 | None:intergenic |
| ATTATCGGAGGAAGAGTTAG+GGG | 0.578446 | 4.3:-4374218 | MS.gene55820:CDS |
| CGGTGGTGGCTGGTATACGG+CGG | 0.578466 | 4.3:+4374975 | None:intergenic |
| GTTCCTTGTTGGGATTGTGG+TGG | 0.579622 | 4.3:-4374028 | MS.gene55820:CDS |
| CGATGTTGTATATATTACTA+CGG | 0.583173 | 4.3:+4374372 | None:intergenic |
| GAGAATAGAAAGATTAAGGA+GGG | 0.583465 | 4.3:-4374443 | MS.gene55820:CDS |
| GGTTGAGATTGTGGTTGTGG+TGG | 0.587118 | 4.3:+4374601 | None:intergenic |
| GAAGGAGTTGTTATTATCGG+AGG | 0.597320 | 4.3:-4374230 | MS.gene55820:CDS |
| TGTAAGTTGGTTACTGCTGG+GGG | 0.598859 | 4.3:-4374001 | MS.gene55820:CDS |
| GGTGTTGGAAGACAAGGGTG+TGG | 0.601057 | 4.3:-4374073 | MS.gene55820:CDS |
| AGAGAGGTGTTGGAAGACAA+GGG | 0.609103 | 4.3:-4374078 | MS.gene55820:CDS |
| GGAAGAGTTAGGGGTTGGTG+TGG | 0.612906 | 4.3:-4374209 | MS.gene55820:CDS |
| AGAGAATAGAAAGATTAAGG+AGG | 0.613300 | 4.3:-4374444 | MS.gene55820:CDS |
| ATGAAACGGGTCGACTTGGG+AGG | 0.616823 | 4.3:-4374123 | MS.gene55820:CDS |
| GATATTGAATGCGACACGTG+TGG | 0.626654 | 4.3:-4374101 | MS.gene55820:CDS |
| CTCCCACCACAATCCCAACA+AGG | 0.638521 | 4.3:+4374025 | None:intergenic |
| TAAACGCCATCGAAGAGCCA+TGG | 0.640163 | 4.3:-4374930 | MS.gene55820:CDS |
| TTGAGGAAGTTGTTGAACAG+CGG | 0.650382 | 4.3:+4374762 | None:intergenic |
| TGTGTTTGATGAGCGTGACG+TGG | 0.654924 | 4.3:-4374281 | MS.gene55820:CDS |
| TCGAACACCGCGAAACTCGT+CGG | 0.663637 | 4.3:+4374955 | None:intergenic |
| ATATATACAACATCGTTGAG+AGG | 0.672095 | 4.3:-4374364 | MS.gene55820:CDS |
| AATGCGACACGTGTGGAGAG+AGG | 0.678434 | 4.3:-4374094 | MS.gene55820:CDS |
| AACACCGCGAAACTCGTCGG+TGG | 0.682439 | 4.3:+4374958 | None:intergenic |
| TGAAAGGAAGCAACCTTACG+TGG | 0.693400 | 4.3:+4374688 | None:intergenic |
| AAGGAAGCAACCTTACGTGG+TGG | 0.725083 | 4.3:+4374691 | None:intergenic |
| TTGTTGATGTTGGTTCAGTG+AGG | 0.729785 | 4.3:-4374567 | MS.gene55820:CDS |
| TGCGATGGTGGTGATGACGG+TGG | 0.737769 | 4.3:+4374724 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CGATGTTGTATATATTACTA+CGG | + | chr4.3:4374586-4374605 | None:intergenic | 25.0% |
| AGACATACTAGAGAAACAAA+AGG | - | chr4.3:4374467-4374486 | MS.gene55820:CDS | 30.0% | |
| AGACTTATTGGAGAATCATA+GGG | - | chr4.3:4374650-4374669 | MS.gene55820:CDS | 30.0% | |
| AGAGAATAGAAAGATTAAGG+AGG | - | chr4.3:4374511-4374530 | MS.gene55820:CDS | 30.0% | |
| ATATATACAACATCGTTGAG+AGG | - | chr4.3:4374591-4374610 | MS.gene55820:CDS | 30.0% | |
| GAGAATAGAAAGATTAAGGA+GGG | - | chr4.3:4374512-4374531 | MS.gene55820:CDS | 30.0% | |
| TCGAGAGAATAGAAAGATTA+AGG | - | chr4.3:4374508-4374527 | MS.gene55820:CDS | 30.0% | |
| TGAAAAATCTCCTCAATCAT+GGG | - | chr4.3:4374137-4374156 | MS.gene55820:CDS | 30.0% | |
| TTTGTCATCATCAAAACTGA+AGG | - | chr4.3:4374425-4374444 | MS.gene55820:CDS | 30.0% | |
| ! | ACTTCATGGAGAGTTTTTAA+AGG | - | chr4.3:4374698-4374717 | MS.gene55820:CDS | 30.0% |
| ! | AGTGAAGGAGTTGTTATTAT+CGG | - | chr4.3:4374722-4374741 | MS.gene55820:CDS | 30.0% |
| !! | AGAGTTTTTAAAGGAAGTGA+AGG | - | chr4.3:4374707-4374726 | MS.gene55820:CDS | 30.0% |
| !!! | AGTTTTGATGATGACAAAGA+TGG | + | chr4.3:4374423-4374442 | None:intergenic | 30.0% |
| AACCCATTCTCATTACACTT+GGG | + | chr4.3:4374993-4375012 | None:intergenic | 35.0% | |
| CAACAAAGCAAGAGACTTAT+TGG | - | chr4.3:4374638-4374657 | MS.gene55820:CDS | 35.0% | |
| CTGAAAAATCTCCTCAATCA+TGG | - | chr4.3:4374136-4374155 | MS.gene55820:CDS | 35.0% | |
| GAGACTTATTGGAGAATCAT+AGG | - | chr4.3:4374649-4374668 | MS.gene55820:CDS | 35.0% | |
| TAACCCATTCTCATTACACT+TGG | + | chr4.3:4374994-4375013 | None:intergenic | 35.0% | |
| TATTATCGGAGGAAGAGTTA+GGG | - | chr4.3:4374736-4374755 | MS.gene55820:CDS | 35.0% | |
| TTATTATCGGAGGAAGAGTT+AGG | - | chr4.3:4374735-4374754 | MS.gene55820:CDS | 35.0% | |
| TTGATGGAGCTTAATGAAAC+GGG | - | chr4.3:4374819-4374838 | MS.gene55820:CDS | 35.0% | |
| TTTCTGGATAGTTGCTTAGT+GGG | + | chr4.3:4374539-4374558 | None:intergenic | 35.0% | |
| TTTGTGAAGGGTAGGTATTT+GGG | - | chr4.3:4374783-4374802 | MS.gene55820:CDS | 35.0% | |
| ! | GGTTAGATTTGTTCCTTGTT+GGG | - | chr4.3:4374917-4374936 | MS.gene55820:CDS | 35.0% |
| ! | GTTGATGGAGCTTAATGAAA+CGG | - | chr4.3:4374818-4374837 | MS.gene55820:CDS | 35.0% |
| ! | TAGAGCAGGTTTTTCTTCTT+GGG | + | chr4.3:4374085-4374104 | None:intergenic | 35.0% |
| ! | TTTTCTGGATAGTTGCTTAG+TGG | + | chr4.3:4374540-4374559 | None:intergenic | 35.0% |
| ACATCAACAACGTTCTTCGT+TGG | + | chr4.3:4374378-4374397 | None:intergenic | 40.0% | |
| ACCCATTCTCATTACACTTG+GGG | + | chr4.3:4374992-4375011 | None:intergenic | 40.0% | |
| ACGAAGAACGTTGTTGATGT+TGG | - | chr4.3:4374378-4374397 | MS.gene55820:CDS | 40.0% | |
| ATTATCGGAGGAAGAGTTAG+GGG | - | chr4.3:4374737-4374756 | MS.gene55820:CDS | 40.0% | |
| CCAGAAAAATGTCCACCAAA+TGG | - | chr4.3:4374552-4374571 | MS.gene55820:CDS | 40.0% | |
| CTAACTTCATCCCATGATTG+AGG | + | chr4.3:4374150-4374169 | None:intergenic | 40.0% | |
| GTTTGTGAAGGGTAGGTATT+TGG | - | chr4.3:4374782-4374801 | MS.gene55820:CDS | 40.0% | |
| TAACTCTTCGAGTGTGTGAA+AGG | + | chr4.3:4374286-4374305 | None:intergenic | 40.0% | |
| TTGAGGAAGTTGTTGAACAG+CGG | + | chr4.3:4374196-4374215 | None:intergenic | 40.0% | |
| TTTGTTCCTTGTTGGGATTG+TGG | - | chr4.3:4374924-4374943 | MS.gene55820:CDS | 40.0% | |
| ! | AGGTTTTTCTTCTTGGGAGA+TGG | + | chr4.3:4374079-4374098 | None:intergenic | 40.0% |
| ! | GAAGGAGTTGTTATTATCGG+AGG | - | chr4.3:4374725-4374744 | MS.gene55820:CDS | 40.0% |
| ! | GGGTTAGATTTGTTCCTTGT+TGG | - | chr4.3:4374916-4374935 | MS.gene55820:CDS | 40.0% |
| ! | GTAGAGCAGGTTTTTCTTCT+TGG | + | chr4.3:4374086-4374105 | None:intergenic | 40.0% |
| ! | TTAATGAAACGGGTCGACTT+GGG | - | chr4.3:4374829-4374848 | MS.gene55820:CDS | 40.0% |
| ! | TTCTTCGTTGGTTGAGATTG+TGG | + | chr4.3:4374366-4374385 | None:intergenic | 40.0% |
| !! | AGTTGTAAGTTGGTTACTGC+TGG | - | chr4.3:4374951-4374970 | MS.gene55820:CDS | 40.0% |
| !! | CCATTTGGTGGACATTTTTC+TGG | + | chr4.3:4374555-4374574 | None:intergenic | 40.0% |
| !! | GAGATTTTTCAGTAGCTTCG+AGG | + | chr4.3:4374128-4374147 | None:intergenic | 40.0% |
| !! | GGGACTCGTTTTTCTTCTTT+GGG | + | chr4.3:4374323-4374342 | None:intergenic | 40.0% |
| !! | GTTGTAAGTTGGTTACTGCT+GGG | - | chr4.3:4374952-4374971 | MS.gene55820:CDS | 40.0% |
| !! | TGGGACTCGTTTTTCTTCTT+TGG | + | chr4.3:4374324-4374343 | None:intergenic | 40.0% |
| !! | TTGTAAGTTGGTTACTGCTG+GGG | - | chr4.3:4374953-4374972 | MS.gene55820:CDS | 40.0% |
| !! | TTGTTGATGTTGGTTCAGTG+AGG | - | chr4.3:4374388-4374407 | MS.gene55820:CDS | 40.0% |
| !!! | TTGTGGTGGTTTTAGTGACT+GGG | + | chr4.3:4374343-4374362 | None:intergenic | 40.0% |
| !!! | TTGTTGAGGTGTTTTAGCCA+TGG | + | chr4.3:4374045-4374064 | None:intergenic | 40.0% |
| CTTAATGAAACGGGTCGACT+TGG | - | chr4.3:4374828-4374847 | MS.gene55820:CDS | 45.0% | |
| GATATTGAATGCGACACGTG+TGG | - | chr4.3:4374854-4374873 | MS.gene55820:CDS | 45.0% | |
| GCCCCAAGTGTAATGAGAAT+GGG | - | chr4.3:4374988-4375007 | MS.gene55820:CDS | 45.0% | |
| GTTGGTTGAGATTGTGGTTG+TGG | + | chr4.3:4374360-4374379 | None:intergenic | 45.0% | |
| TGAAAGGAAGCAACCTTACG+TGG | + | chr4.3:4374270-4374289 | None:intergenic | 45.0% | |
| TGAAGGGTAGGTATTTGGGT+GGG | - | chr4.3:4374787-4374806 | MS.gene55820:CDS | 45.0% | |
| TGAGGAAGTTGTTGAACAGC+GGG | + | chr4.3:4374195-4374214 | None:intergenic | 45.0% | |
| TGCCCCAAGTGTAATGAGAA+TGG | - | chr4.3:4374987-4375006 | MS.gene55820:CDS | 45.0% | |
| TTACTGCTGGGGGTGAAATA+AGG | - | chr4.3:4374964-4374983 | MS.gene55820:CDS | 45.0% | |
| TTCCTTGTTGGGATTGTGGT+GGG | - | chr4.3:4374928-4374947 | MS.gene55820:CDS | 45.0% | |
| TTTCAGTAGCTTCGAGGATG+TGG | + | chr4.3:4374122-4374141 | None:intergenic | 45.0% | |
| ! | TTGTGGTGGGAGTTGTAAGT+TGG | - | chr4.3:4374941-4374960 | MS.gene55820:CDS | 45.0% |
| ! | TTTTAGCCATGGCTCTTCGA+TGG | + | chr4.3:4374034-4374053 | None:intergenic | 45.0% |
| !! | AGAGAGGTGTTGGAAGACAA+GGG | - | chr4.3:4374877-4374896 | MS.gene55820:CDS | 45.0% |
| !! | TGGTGTTGTTGGTGTTGTTG+AGG | + | chr4.3:4374059-4374078 | None:intergenic | 45.0% |
| !! | TGTAAGTTGGTTACTGCTGG+GGG | - | chr4.3:4374954-4374973 | MS.gene55820:CDS | 45.0% |
| !! | TTCTTGGGAGATGGTGTTGT+TGG | + | chr4.3:4374070-4374089 | None:intergenic | 45.0% |
| !! | TTGCCAAGGGTGTTTGTGAA+GGG | - | chr4.3:4374771-4374790 | MS.gene55820:CDS | 45.0% |
| !!! | GTTGTGGTGGTTTTAGTGAC+TGG | + | chr4.3:4374344-4374363 | None:intergenic | 45.0% |
| AAGGAAGCAACCTTACGTGG+TGG | + | chr4.3:4374267-4374286 | None:intergenic | 50.0% | |
| ACAACCACAACCACCACGTA+AGG | - | chr4.3:4374254-4374273 | MS.gene55820:CDS | 50.0% | |
| ACTACGGCGTCGTTTCCATT+TGG | + | chr4.3:4374570-4374589 | None:intergenic | 50.0% | |
| AGAATAGGAGGAGGTAGAGC+AGG | + | chr4.3:4374099-4374118 | None:intergenic | 50.0% | |
| AGGATGTGGAGAATAGGAGG+AGG | + | chr4.3:4374108-4374127 | None:intergenic | 50.0% | |
| CTACCCTTCACAAACACCCT+TGG | + | chr4.3:4374777-4374796 | None:intergenic | 50.0% | |
| GCTTCGAGGATGTGGAGAAT+AGG | + | chr4.3:4374114-4374133 | None:intergenic | 50.0% | |
| GGTTGAGATTGTGGTTGTGG+TGG | + | chr4.3:4374357-4374376 | None:intergenic | 50.0% | |
| GTGAAGGGTAGGTATTTGGG+TGG | - | chr4.3:4374786-4374805 | MS.gene55820:CDS | 50.0% | |
| GTTCCTTGTTGGGATTGTGG+TGG | - | chr4.3:4374927-4374946 | MS.gene55820:CDS | 50.0% | |
| TAAACGCCATCGAAGAGCCA+TGG | - | chr4.3:4374025-4374044 | MS.gene55820:CDS | 50.0% | |
| TCGAGGATGTGGAGAATAGG+AGG | + | chr4.3:4374111-4374130 | None:intergenic | 50.0% | |
| TGTGTTTGATGAGCGTGACG+TGG | - | chr4.3:4374674-4374693 | MS.gene55820:CDS | 50.0% | |
| ! | TGTTGCGATGGTGGTGATGA+CGG | + | chr4.3:4374237-4374256 | None:intergenic | 50.0% |
| !! | CAAGGGTGTTTGTGAAGGGT+AGG | - | chr4.3:4374775-4374794 | MS.gene55820:CDS | 50.0% |
| !! | CTTGCCAAGGGTGTTTGTGA+AGG | - | chr4.3:4374770-4374789 | MS.gene55820:CDS | 50.0% |
| !! | GAACAGCGGGTTTGAGTTGT+TGG | + | chr4.3:4374182-4374201 | None:intergenic | 50.0% |
| !! | GAGAGAGGTGTTGGAAGACA+AGG | - | chr4.3:4374876-4374895 | MS.gene55820:CDS | 50.0% |
| AATGCGACACGTGTGGAGAG+AGG | - | chr4.3:4374861-4374880 | MS.gene55820:CDS | 55.0% | |
| ACGGCGTCGTTTCCATTTGG+TGG | + | chr4.3:4374567-4374586 | None:intergenic | 55.0% | |
| CTCCCACCACAATCCCAACA+AGG | + | chr4.3:4374933-4374952 | None:intergenic | 55.0% | |
| GCAACCTTACGTGGTGGTTG+TGG | + | chr4.3:4374261-4374280 | None:intergenic | 55.0% | |
| GGGTAGGTATTTGGGTGGGT+TGG | - | chr4.3:4374791-4374810 | MS.gene55820:CDS | 55.0% | |
| GGTGGTTGTGGTTGTTGCGA+TGG | + | chr4.3:4374249-4374268 | None:intergenic | 55.0% | |
| GGTTGTGGTTGTTGCGATGG+TGG | + | chr4.3:4374246-4374265 | None:intergenic | 55.0% | |
| TCGAACACCGCGAAACTCGT+CGG | + | chr4.3:4374003-4374022 | None:intergenic | 55.0% | |
| TCGGAGGAAGAGTTAGGGGT+TGG | - | chr4.3:4374741-4374760 | MS.gene55820:CDS | 55.0% | |
| ! | ACACGTGTGGAGAGAGGTGT+TGG | - | chr4.3:4374867-4374886 | MS.gene55820:CDS | 55.0% |
| ! | GGTGTTGGAAGACAAGGGTG+TGG | - | chr4.3:4374882-4374901 | MS.gene55820:CDS | 55.0% |
| ! | GGTTGGTGTGGTCTTGCCAA+GGG | - | chr4.3:4374758-4374777 | MS.gene55820:CDS | 55.0% |
| ! | GTTGGAAGACAAGGGTGTGG+TGG | - | chr4.3:4374885-4374904 | MS.gene55820:CDS | 55.0% |
| !! | AAGGGTGTGGTGGATGTGGT+GGG | - | chr4.3:4374895-4374914 | MS.gene55820:CDS | 55.0% |
| !! | AGACAAGGGTGTGGTGGATG+TGG | - | chr4.3:4374891-4374910 | MS.gene55820:CDS | 55.0% |
| !! | ATGAAACGGGTCGACTTGGG+AGG | - | chr4.3:4374832-4374851 | MS.gene55820:CDS | 55.0% |
| !! | GGAAGAGTTAGGGGTTGGTG+TGG | - | chr4.3:4374746-4374765 | MS.gene55820:CDS | 55.0% |
| !! | GGGTGGGTTGGATGAGTTGA+TGG | - | chr4.3:4374803-4374822 | MS.gene55820:CDS | 55.0% |
| !! | GGTGATGGTGGTGTTGGTTG+AGG | + | chr4.3:4374213-4374232 | None:intergenic | 55.0% |
| AACACCGCGAAACTCGTCGG+TGG | + | chr4.3:4374000-4374019 | None:intergenic | 60.0% | |
| GAGCGTGACGTGGCACTTCA+TGG | - | chr4.3:4374684-4374703 | MS.gene55820:CDS | 60.0% | |
| ! | CGTCGGTGGTGGCTGGTATA+CGG | + | chr4.3:4373986-4374005 | None:intergenic | 60.0% |
| !! | AGGGTGTGGTGGATGTGGTG+GGG | - | chr4.3:4374896-4374915 | MS.gene55820:CDS | 60.0% |
| !! | CAAGGGTGTGGTGGATGTGG+TGG | - | chr4.3:4374894-4374913 | MS.gene55820:CDS | 60.0% |
| !! | GACGGTGGTGATGGTGGTGT+TGG | + | chr4.3:4374219-4374238 | None:intergenic | 60.0% |
| !! | GGGTTGGTGTGGTCTTGCCA+AGG | - | chr4.3:4374757-4374776 | MS.gene55820:CDS | 60.0% |
| !! | GGTGATGACGGTGGTGATGG+TGG | + | chr4.3:4374225-4374244 | None:intergenic | 60.0% |
| !! | GGTGGTGATGACGGTGGTGA+TGG | + | chr4.3:4374228-4374247 | None:intergenic | 60.0% |
| !! | TGCGATGGTGGTGATGACGG+TGG | + | chr4.3:4374234-4374253 | None:intergenic | 60.0% |
| ACCGCGAAACTCGTCGGTGG+TGG | + | chr4.3:4373997-4374016 | None:intergenic | 65.0% | |
| GCCACCACCGACGAGTTTCG+CGG | - | chr4.3:4373993-4374012 | MS.gene55820:CDS | 65.0% | |
| TGGCTGGTATACGGCGGGTG+CGG | + | chr4.3:4373977-4373996 | None:intergenic | 65.0% | |
| ! | CGAAACTCGTCGGTGGTGGC+TGG | + | chr4.3:4373993-4374012 | None:intergenic | 65.0% |
| ! | CGGTGGTGGCTGGTATACGG+CGG | + | chr4.3:4373983-4374002 | None:intergenic | 65.0% |
| ! | GGTGGTGGCTGGTATACGGC+GGG | + | chr4.3:4373982-4374001 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 4373940 | 4375037 | 4373940 | ID=MS.gene55820 |
| chr4.3 | mRNA | 4373940 | 4375037 | 4373940 | ID=MS.gene55820.t1;Parent=MS.gene55820 |
| chr4.3 | exon | 4373940 | 4375037 | 4373940 | ID=MS.gene55820.t1.exon1;Parent=MS.gene55820.t1 |
| chr4.3 | CDS | 4373940 | 4375037 | 4373940 | ID=cds.MS.gene55820.t1;Parent=MS.gene55820.t1 |
| Gene Sequence |
| Protein sequence |
