AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58329


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58329.t1 MTR_8g032300 98.649 148 2 0 1 148 1 148 2.50e-107 302
MS.gene58329.t1 MTR_8g032320 83.108 148 25 0 1 148 1 148 1.16e-92 265
MS.gene58329.t1 MTR_1g008950 64.000 125 45 0 1 125 1 125 2.08e-57 175
MS.gene58329.t1 MTR_5g079880 40.645 155 77 5 1 144 1 151 4.64e-35 119
MS.gene58329.t1 MTR_5g079890 39.241 158 80 5 1 145 1 155 4.49e-32 112
MS.gene58329.t1 MTR_5g079890 39.130 138 71 4 1 125 1 138 8.96e-29 103
MS.gene58329.t1 MTR_7g075960 60.377 53 21 0 25 77 1 53 1.09e-20 80.1
MS.gene58329.t1 MTR_8g030870 28.571 147 93 2 1 135 1 147 2.41e-16 72.4
MS.gene58329.t1 MTR_3g106660 32.283 127 82 2 3 128 439 562 1.03e-13 67.4
MS.gene58329.t1 MTR_5g004800 27.119 118 84 2 6 122 15 131 2.29e-12 62.8
MS.gene58329.t1 MTR_4g115660 33.588 131 81 4 3 131 440 566 2.84e-12 63.5
MS.gene58329.t1 MTR_5g004870 29.412 119 82 2 6 123 11 128 4.72e-12 62.0
MS.gene58329.t1 MTR_2g027100 37.363 91 55 2 4 94 6 94 5.71e-12 60.5
MS.gene58329.t1 MTR_2g030230 37.363 91 55 2 4 94 173 261 3.60e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58329.t1 AT1G63220 57.432 148 62 1 1 148 1 147 9.25e-56 172
MS.gene58329.t1 AT1G63220 52.027 148 56 2 1 148 1 133 1.32e-45 145
MS.gene58329.t1 AT3G55470 34.177 158 90 5 1 147 1 155 1.55e-23 90.1
MS.gene58329.t1 AT3G55470 34.591 159 89 6 1 147 1 156 1.45e-22 87.8
MS.gene58329.t1 AT4G00467 30.282 142 88 3 1 131 1 142 4.67e-15 68.9
MS.gene58329.t1 AT4G00467 30.282 142 88 3 1 131 1 142 7.48e-15 68.9
MS.gene58329.t1 AT5G47710 39.785 93 54 2 2 94 4 94 1.02e-13 65.1
MS.gene58329.t1 AT5G47710 39.785 93 54 2 2 94 4 94 1.02e-13 65.1
MS.gene58329.t1 AT5G47710 39.785 93 54 2 2 94 4 94 1.02e-13 65.1
MS.gene58329.t1 AT5G47710 39.785 93 54 2 2 94 4 94 1.02e-13 65.1
MS.gene58329.t1 AT5G04220 32.278 158 92 5 2 147 37 191 4.51e-13 65.5
MS.gene58329.t1 AT5G04220 32.278 158 92 5 2 147 197 351 9.47e-13 64.7
MS.gene58329.t1 AT5G04220 32.278 158 92 5 2 147 259 413 1.39e-12 64.3
MS.gene58329.t1 AT4G34150 26.613 124 89 2 6 128 12 134 3.94e-12 62.0

Find 32 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATGTGATCCTCACTTAT+AGG 0.237814 8.2:+65810360 MS.gene58329:CDS
TGAGCTCAATCTAAAGATAA+TGG 0.346296 8.2:+65810571 MS.gene58329:CDS
ACAAGTTTGTAAGAATTCTC+TGG 0.370783 8.2:-65810962 None:intergenic
AGAGAAAATCATTGTCTTCA+AGG 0.372599 8.2:-65809630 None:intergenic
TTATGTGATCCTCACTTATA+GGG 0.386347 8.2:+65810361 MS.gene58329:CDS
AAAATATTGTGGAGAGGTTA+AGG 0.388006 8.2:+65810994 MS.gene58329:CDS
TATAGTGCTGATGATAATCT+TGG 0.411031 8.2:+65810605 MS.gene58329:CDS
GAAATGTCTCATTCCATTGA+GGG 0.473516 8.2:-65810523 None:intergenic
CAATCTAAAGATAATGGAAA+AGG 0.492449 8.2:+65810577 MS.gene58329:CDS
TCTTGAATCACTGTCTCTAA+AGG 0.495815 8.2:-65810929 None:intergenic
GTGCTGGATCCAACCCTCAA+TGG 0.516268 8.2:+65810510 MS.gene58329:CDS
AGAAATGTCTCATTCCATTG+AGG 0.522164 8.2:-65810524 None:intergenic
TGTCTCATTCCATTGAGGGT+TGG 0.547968 8.2:-65810519 None:intergenic
GTGGATGGAAACAATCGGCT+AGG 0.550623 8.2:+65811178 MS.gene58329:CDS
CTATAAGTGAGGATCACATA+AGG 0.556013 8.2:-65810359 None:intergenic
TACAATCAAGAGGAGGAGAC+TGG 0.557679 8.2:+65811156 MS.gene58329:CDS
AAGAGGAGGAGACTGGTGGA+TGG 0.563015 8.2:+65811163 MS.gene58329:CDS
GACTGGTGGATGGAAACAAT+CGG 0.565129 8.2:+65811173 MS.gene58329:CDS
GATCCTCACTTATAGGGCAC+AGG 0.584755 8.2:+65810367 MS.gene58329:CDS
CACAAGAGTACTGTGCAAGA+AGG 0.593988 8.2:+65810392 MS.gene58329:CDS
GAATTCTTACAAACTTGTGA+AGG 0.595780 8.2:+65810967 MS.gene58329:CDS
TGGATGGAAACAATCGGCTA+GGG 0.602214 8.2:+65811179 MS.gene58329:CDS
AATCAAGAGGAGGAGACTGG+TGG 0.628097 8.2:+65811159 MS.gene58329:CDS
TTTAGAGACAGTGATTCAAG+AGG 0.636421 8.2:+65810931 MS.gene58329:CDS
TGAACGGAGTTACAATCAAG+AGG 0.638924 8.2:+65811146 MS.gene58329:CDS
TTAGAGACAGTGATTCAAGA+GGG 0.640617 8.2:+65810932 MS.gene58329:CDS
GCTCCTGTGCCCTATAAGTG+AGG 0.645283 8.2:-65810370 None:intergenic
GGTTGCAGAGAAACTATGAA+CGG 0.677708 8.2:+65811130 MS.gene58329:intron
AATCAGAGAAAGATGCCTCG+TGG 0.689332 8.2:+65809574 None:intergenic
ATATTGTGGAGAGGTTAAGG+TGG 0.690996 8.2:+65810997 MS.gene58329:CDS
ACGGAGTTACAATCAAGAGG+AGG 0.705054 8.2:+65811149 MS.gene58329:CDS
ACAACTTCAAGCGTTCCACG+AGG 0.769497 8.2:-65809589 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTGAGAAAAA+GGG - chr8.2:65810231-65810250 None:intergenic 10.0%
!!! TTTTTTTTTTTTTGAGAAAA+AGG - chr8.2:65810232-65810251 None:intergenic 10.0%
!! AAGAAAAATATTAGAAAAAC+AGG - chr8.2:65811060-65811079 None:intergenic 15.0%
!!! AACAATTTTAATAGTCAAAT+TGG - chr8.2:65810282-65810301 None:intergenic 15.0%
!! ATAATATGATAAAACCAAAC+CGG - chr8.2:65810072-65810091 None:intergenic 20.0%
!! ATTGTATCATGAATAATATC+AGG + chr8.2:65810488-65810507 MS.gene58329:intron 20.0%
!!! AATGTTGTAATTTGTAATGT+TGG + chr8.2:65810883-65810902 MS.gene58329:intron 20.0%
!!! CATGAGTTTTTATTGAATAT+CGG - chr8.2:65810730-65810749 None:intergenic 20.0%
!!! GAAATTTTTTGATCTTAAGT+TGG + chr8.2:65809747-65809766 MS.gene58329:intron 20.0%
! AAAAAAGATCAAGAGTTTAG+AGG - chr8.2:65810316-65810335 None:intergenic 25.0%
! GAAATTTATGCATCAATCAA+TGG + chr8.2:65809685-65809704 MS.gene58329:intron 25.0%
! GGAACAACAAATATATAGAT+CGG - chr8.2:65809774-65809793 None:intergenic 25.0%
! GTGTTGTATGCTATTAAATT+GGG + chr8.2:65810105-65810124 MS.gene58329:intron 25.0%
! TAAAAACTCATGATATTGCA+AGG + chr8.2:65810738-65810757 MS.gene58329:intron 25.0%
! TAAAACGCACTATCAATTAT+TGG + chr8.2:65811109-65811128 MS.gene58329:intron 25.0%
! TAACTGAAAAAGTTGAATGT+TGG + chr8.2:65810790-65810809 MS.gene58329:intron 25.0%
! TGTGTTGTATGCTATTAAAT+TGG + chr8.2:65810104-65810123 MS.gene58329:intron 25.0%
! TTAAACTCAAGATGAATCAA+AGG - chr8.2:65810673-65810692 None:intergenic 25.0%
! TTAAATTGTAGTGAGAAAGT+TGG - chr8.2:65810768-65810787 None:intergenic 25.0%
! TTGATTCATCTTGAGTTTAA+TGG + chr8.2:65810673-65810692 MS.gene58329:intron 25.0%
!! AACTAGTTTACACTTAAAAG+AGG + chr8.2:65810856-65810875 MS.gene58329:intron 25.0%
!!! AATTGATAGTGCGTTTTAAT+AGG - chr8.2:65811107-65811126 None:intergenic 25.0%
!!! ATTGATAGTGCGTTTTAATA+GGG - chr8.2:65811106-65811125 None:intergenic 25.0%
!!! CAATCTAAAGATAATGGAAA+AGG + chr8.2:65810577-65810596 MS.gene58329:CDS 25.0%
AAAATATTGTGGAGAGGTTA+AGG + chr8.2:65810994-65811013 MS.gene58329:CDS 30.0%
AAACGAACACTATAACAAGA+CGG - chr8.2:65809961-65809980 None:intergenic 30.0%
AATGTTGACAGCTAAATCAT+TGG - chr8.2:65810026-65810045 None:intergenic 30.0%
ACAAGTTTGTAAGAATTCTC+TGG - chr8.2:65810965-65810984 None:intergenic 30.0%
AGACTCAATTAACAAGCTTT+AGG - chr8.2:65809795-65809814 None:intergenic 30.0%
AGAGAAAATCATTGTCTTCA+AGG - chr8.2:65809633-65809652 None:intergenic 30.0%
CATCGCTAAAAACGAAAAAA+AGG - chr8.2:65810157-65810176 None:intergenic 30.0%
CTGATTTAGTGAATCAATTC+AGG + chr8.2:65809826-65809845 MS.gene58329:intron 30.0%
CTTGTCATAAGATTCAGAAA+TGG - chr8.2:65810419-65810438 None:intergenic 30.0%
GAATTCTTACAAACTTGTGA+AGG + chr8.2:65810967-65810986 MS.gene58329:CDS 30.0%
GTGAAGGATGAAAAATATTG+TGG + chr8.2:65810983-65811002 MS.gene58329:CDS 30.0%
TGAGCTCAATCTAAAGATAA+TGG + chr8.2:65810571-65810590 MS.gene58329:CDS 30.0%
TTATGTGATCCTCACTTATA+GGG + chr8.2:65810361-65810380 MS.gene58329:CDS 30.0%
! ATCACAAGATTTCACACTTT+TGG - chr8.2:65809713-65809732 None:intergenic 30.0%
! CTGAATTGATTCACTAAATC+AGG - chr8.2:65809828-65809847 None:intergenic 30.0%
!! TATAGTGCTGATGATAATCT+TGG + chr8.2:65810605-65810624 MS.gene58329:CDS 30.0%
AGAAATGTCTCATTCCATTG+AGG - chr8.2:65810527-65810546 None:intergenic 35.0%
CTATAAGTGAGGATCACATA+AGG - chr8.2:65810362-65810381 None:intergenic 35.0%
CTTATGTGATCCTCACTTAT+AGG + chr8.2:65810360-65810379 MS.gene58329:CDS 35.0%
GAAATGTCTCATTCCATTGA+GGG - chr8.2:65810526-65810545 None:intergenic 35.0%
GAGTACATTGAATACAGTCT+CGG - chr8.2:65810186-65810205 None:intergenic 35.0%
GGATGAAAAATATTGTGGAG+AGG + chr8.2:65810988-65811007 MS.gene58329:CDS 35.0%
TCATGAATAATATCAGGTGC+TGG + chr8.2:65810494-65810513 MS.gene58329:intron 35.0%
TCTTGAATCACTGTCTCTAA+AGG - chr8.2:65810932-65810951 None:intergenic 35.0%
TTAGAGACAGTGATTCAAGA+GGG + chr8.2:65810932-65810951 MS.gene58329:CDS 35.0%
TTTAGAGACAGTGATTCAAG+AGG + chr8.2:65810931-65810950 MS.gene58329:CDS 35.0%
!!! GTTGTTTTGATCAGTGCTAA+AGG + chr8.2:65809607-65809626 MS.gene58329:CDS 35.0%
ATATTGTGGAGAGGTTAAGG+TGG + chr8.2:65810997-65811016 MS.gene58329:CDS 40.0%
GGTTGCAGAGAAACTATGAA+CGG + chr8.2:65811130-65811149 MS.gene58329:intron 40.0%
TAATAGCCCAATTCACGAGT+CGG + chr8.2:65809895-65809914 MS.gene58329:intron 40.0%
TGAACGGAGTTACAATCAAG+AGG + chr8.2:65811146-65811165 MS.gene58329:CDS 40.0%
TGTCAACATTGCATAACCCT+AGG + chr8.2:65810036-65810055 MS.gene58329:intron 40.0%
TTCTATAGCCTCATTACCTC+AGG - chr8.2:65811037-65811056 None:intergenic 40.0%
! CTTTTACACCTGAGGTAATG+AGG + chr8.2:65811026-65811045 MS.gene58329:intron 40.0%
AAACCGGAATACTCATGCCT+AGG - chr8.2:65810056-65810075 None:intergenic 45.0%
AACCCTAGGCATGAGTATTC+CGG + chr8.2:65810050-65810069 MS.gene58329:intron 45.0%
AACCGGAATACTCATGCCTA+GGG - chr8.2:65810055-65810074 None:intergenic 45.0%
ACGGAGTTACAATCAAGAGG+AGG + chr8.2:65811149-65811168 MS.gene58329:CDS 45.0%
CACCACGACGATGAACAATA+AGG - chr8.2:65809921-65809940 None:intergenic 45.0%
TACAATCAAGAGGAGGAGAC+TGG + chr8.2:65811156-65811175 MS.gene58329:CDS 45.0%
TAGGCATGAGTATTCCGGTT+TGG + chr8.2:65810055-65810074 MS.gene58329:intron 45.0%
TGGATGGAAACAATCGGCTA+GGG + chr8.2:65811179-65811198 MS.gene58329:CDS 45.0%
TGTCTCATTCCATTGAGGGT+TGG - chr8.2:65810522-65810541 None:intergenic 45.0%
! GACTGGTGGATGGAAACAAT+CGG + chr8.2:65811173-65811192 MS.gene58329:CDS 45.0%
!! AATAAGGCCGACTCGTGAAT+TGG - chr8.2:65809905-65809924 None:intergenic 45.0%
!! ATAAGGCCGACTCGTGAATT+GGG - chr8.2:65809904-65809923 None:intergenic 45.0%
!! CACAAGAGTACTGTGCAAGA+AGG + chr8.2:65810392-65810411 MS.gene58329:CDS 45.0%
AATCAAGAGGAGGAGACTGG+TGG + chr8.2:65811159-65811178 MS.gene58329:CDS 50.0%
ACAACTTCAAGCGTTCCACG+AGG - chr8.2:65809592-65809611 None:intergenic 50.0%
GATCCTCACTTATAGGGCAC+AGG + chr8.2:65810367-65810386 MS.gene58329:CDS 50.0%
GTGGATGGAAACAATCGGCT+AGG + chr8.2:65811178-65811197 MS.gene58329:CDS 50.0%
!! GGCCTTATTGTTCATCGTCG+TGG + chr8.2:65809916-65809935 MS.gene58329:intron 50.0%
!! GGCTCTCACTTTTACACCTG+AGG + chr8.2:65811018-65811037 MS.gene58329:CDS 50.0%
AAGAGGAGGAGACTGGTGGA+TGG + chr8.2:65811163-65811182 MS.gene58329:CDS 55.0%
GCTCCTGTGCCCTATAAGTG+AGG - chr8.2:65810373-65810392 None:intergenic 55.0%
GTGCTGGATCCAACCCTCAA+TGG + chr8.2:65810510-65810529 MS.gene58329:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 65809586 65811209 65809586 ID=MS.gene58329
chr8.2 mRNA 65809586 65811209 65809586 ID=MS.gene58329.t1;Parent=MS.gene58329
chr8.2 exon 65809586 65809652 65809586 ID=MS.gene58329.t1.exon1;Parent=MS.gene58329.t1
chr8.2 CDS 65809586 65809652 65809586 ID=cds.MS.gene58329.t1;Parent=MS.gene58329.t1
chr8.2 exon 65810348 65810413 65810348 ID=MS.gene58329.t1.exon2;Parent=MS.gene58329.t1
chr8.2 CDS 65810348 65810413 65810348 ID=cds.MS.gene58329.t1;Parent=MS.gene58329.t1
chr8.2 exon 65810510 65810636 65810510 ID=MS.gene58329.t1.exon3;Parent=MS.gene58329.t1
chr8.2 CDS 65810510 65810636 65810510 ID=cds.MS.gene58329.t1;Parent=MS.gene58329.t1
chr8.2 exon 65810925 65811039 65810925 ID=MS.gene58329.t1.exon4;Parent=MS.gene58329.t1
chr8.2 CDS 65810925 65811039 65810925 ID=cds.MS.gene58329.t1;Parent=MS.gene58329.t1
chr8.2 exon 65811138 65811209 65811138 ID=MS.gene58329.t1.exon5;Parent=MS.gene58329.t1
chr8.2 CDS 65811138 65811209 65811138 ID=cds.MS.gene58329.t1;Parent=MS.gene58329.t1
Gene Sequence

>MS.gene58329

ATGCCTCGTGGAACGCTTGAAGTTGTTTTGATCAGTGCTAAAGGCCTTGAAGACAATGATTTTCTCTGTAAGTAAAGTAATATTATGTACATTCTCATTGAAATTTATGCATCAATCAATGGATCCAAAAGTGTGAAATCTTGTGATCAAATTAATCTATAGAAATTTTTTGATCTTAAGTTGGTCCGATCTATATATTTGTTGTTCCTAAAGCTTGTTAATTGAGTCTATTTTTCATTCCTGATTTAGTGAATCAATTCAGGTGACTTTTTCTTTGTTAATTTCTAATTAGTTGTAACTATAATCATATAATAGCCCAATTCACGAGTCGGCCTTATTGTTCATCGTCGTGGTGCGATGTTGTTTGATTTTCCGTCTTGTTATAGTGTTCGTTTGATTAATATTGAAAGATCAGCTTTAATCAATTATTGATTCAACCAATGATTTAGCTGTCAACATTGCATAACCCTAGGCATGAGTATTCCGGTTTGGTTTTATCATATTATTTGTAGATATTTTGTGTTGTATGCTATTAAATTGGGTGTTGCGAGTTTGTTTGCAGATTCATCCTTTTTTTCGTTTTTAGCGATGTTGAATCCGAGACTGTATTCAATGTACTCTATCAATATATATGAACGAATACCCTTTTTCTCAAAAAAAAAAAAACTAAAATCATATAATAATGTTGAATTACCAATTTGACTATTAAAATTGTTCTAGATATGATCCTCTAAACTCTTGATCTTTTTTCTGAATGAGCAGCTAGCATAGATCCTTATGTGATCCTCACTTATAGGGCACAGGAGCACAAGAGTACTGTGCAAGAAGGTCCATTTCTGAATCTTATGACAAGTATTAATCTGCTTATTTGTTAGTTTTTTTTTCTCTCTAGTTGCTTGATTATTGTATCATGAATAATATCAGGTGCTGGATCCAACCCTCAATGGAATGAGACATTTCTTTTCACTGTTTCTGACACTGCTTCTGAGCTCAATCTAAAGATAATGGAAAAGGACAACTATAGTGCTGATGATAATCTTGGAGAAGTAATGTGAGTTAAATTCTTCTTATACTTGCAAATTTTCCTTTGATTCATCTTGAGTTTAATGGAGATACATTGTCAGTGTAAAATAGTATTACACCGATATTCAATAAAAACTCATGATATTGCAAGGTAAACCAACTTTCTCACTACAATTTAAAATAACTGAAAAAGTTGAATGTTGGTGTAAAACTAGTTTATATTGATAGTGTACACTTCTTTTCTTAAAACTAGTTTACACTTAAAAGAGGTCAGAATGTTGTAATTTGTAATGTTGGCATTCATTTCTGTCTATAGAATTCCTTTAGAGACAGTGATTCAAGAGGGTAGCGTTCCAGAGAATTCTTACAAACTTGTGAAGGATGAAAAATATTGTGGAGAGGTTAAGGTGGCTCTCACTTTTACACCTGAGGTAATGAGGCTATAGAACCTGTTTTTCTAATATTTTTCTTCATACTTTTCTGCATAATTTTCTCCCTATTAAAACGCACTATCAATTATTGGTTGCAGAGAAACTATGAACGGAGTTACAATCAAGAGGAGGAGACTGGTGGATGGAAACAATCGGCTAGGGACTATTGA

Protein sequence

>MS.gene58329.t1

MPRGTLEVVLISAKGLEDNDFLSSIDPYVILTYRAQEHKSTVQEGAGSNPQWNETFLFTVSDTASELNLKIMEKDNYSADDNLGEVIIPLETVIQEGSVPENSYKLVKDEKYCGEVKVALTFTPERNYERSYNQEEETGGWKQSARDY