AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65822


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65822.t1 MTR_3g018925 92.784 97 7 0 4 100 13 109 1.02e-62 186
MS.gene65822.t1 MTR_3g018790 90.000 100 10 0 1 100 161 260 6.36e-61 186
MS.gene65822.t1 MTR_3g018790 90.000 100 10 0 1 100 215 314 1.42e-60 187
MS.gene65822.t1 MTR_8g038220 68.000 100 32 0 1 100 215 314 1.71e-44 146
MS.gene65822.t1 MTR_8g038220 68.000 100 32 0 1 100 214 313 1.78e-44 146
MS.gene65822.t1 MTR_8g038180 67.347 98 32 0 1 98 215 312 1.33e-42 141
MS.gene65822.t1 MTR_8g038210 69.000 100 31 0 1 100 214 313 3.74e-42 140
MS.gene65822.t1 MTR_8g038210 69.000 100 31 0 1 100 214 313 4.35e-42 140
MS.gene65822.t1 MTR_3g018780 66.000 100 34 0 1 100 215 314 7.06e-42 139
MS.gene65822.t1 MTR_0276s0050 52.273 88 42 0 13 100 228 315 6.82e-24 92.8
MS.gene65822.t1 MTR_2g031980 44.444 99 55 0 1 99 215 313 4.95e-22 87.8
MS.gene65822.t1 MTR_5g063670 39.785 93 56 0 5 97 220 312 3.76e-19 80.1
MS.gene65822.t1 MTR_1g033560 39.785 93 56 0 5 97 220 312 5.33e-19 79.7
MS.gene65822.t1 MTR_8g107640 41.000 100 59 0 1 100 216 315 1.56e-18 78.2
MS.gene65822.t1 MTR_1g112520 43.956 91 51 0 7 97 222 312 1.63e-17 75.5
MS.gene65822.t1 MTR_6g071605 42.857 91 52 0 7 97 222 312 1.93e-17 75.5
MS.gene65822.t1 MTR_6g071615 41.237 97 57 0 1 97 216 312 1.13e-16 73.2
MS.gene65822.t1 MTR_8g038150 41.111 90 53 0 9 98 227 316 7.34e-16 71.2
MS.gene65822.t1 MTR_6g071595 41.758 91 53 0 7 97 222 312 8.73e-15 68.2
MS.gene65822.t1 MTR_8g038090 50.000 72 31 2 30 98 22 91 3.06e-14 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65822.t1 AT5G12380 56.701 97 42 0 1 97 215 311 1.06e-33 118
MS.gene65822.t1 AT5G10220 49.474 95 48 0 5 99 222 316 1.82e-25 97.1
MS.gene65822.t1 AT5G10230 45.361 97 53 0 1 97 216 312 2.78e-23 91.3
MS.gene65822.t1 AT5G65020 43.878 98 54 1 1 97 201 298 1.49e-21 86.3
MS.gene65822.t1 AT5G65020 43.878 98 54 1 1 97 216 313 1.65e-21 86.3
MS.gene65822.t1 AT2G38760 42.222 90 52 0 8 97 229 318 2.23e-20 83.6
MS.gene65822.t1 AT1G35720 44.444 90 50 0 11 100 227 316 5.75e-20 82.4
MS.gene65822.t1 AT1G68090 35.052 97 63 0 1 97 217 313 6.86e-14 65.9

Find 24 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCAAGTCCTTCTCAGCCC+TGG 0.436107 3.2:-9314269 None:intergenic
GAAATTTCTCCTTACTCTGA+TGG 0.445732 3.2:+9314372 MS.gene65822:CDS
TTGCAGAAATTGTCCGATGA+AGG 0.446312 3.2:+9313609 MS.gene65822:intron
ATGGTCATTGATGCAGCTAA+TGG 0.457405 3.2:-9313664 None:intergenic
CTTCTCTCCCCATCAGAGTA+AGG 0.466949 3.2:-9314381 None:intergenic
AAATTTCTCCTTACTCTGAT+GGG 0.467181 3.2:+9314373 MS.gene65822:CDS
CACCAGGGCTGAGAAGGACT+TGG 0.474764 3.2:+9314267 MS.gene65822:CDS
CGCAATGCGATGGAAACTGT+TGG 0.526003 3.2:+9314211 MS.gene65822:CDS
CCATAATAAGTACTATGAAA+AGG 0.528583 3.2:+9313683 MS.gene65822:CDS
GATTGTCACCAGGGCTGAGA+AGG 0.535488 3.2:+9314261 MS.gene65822:CDS
TGGCCACTGCATGCTCAAGT+TGG 0.542716 3.2:-9314327 None:intergenic
CAGGGCTGAGAAGGACTTGG+AGG 0.543973 3.2:+9314270 MS.gene65822:CDS
GGACTTGGAGGACATAAGAA+AGG 0.590987 3.2:+9314282 MS.gene65822:CDS
GTAGGTTCTACGCAATGCGA+TGG 0.594107 3.2:+9314201 MS.gene65822:intron
TCACCCGTGTGATTGTCACC+AGG 0.598018 3.2:+9314251 MS.gene65822:CDS
AATTTCTCCTTACTCTGATG+GGG 0.599634 3.2:+9314374 MS.gene65822:CDS
CAGCCCTGGTGACAATCACA+CGG 0.607678 3.2:-9314255 None:intergenic
AAAGCTATCAGATCCTTCAT+CGG 0.630218 3.2:-9313622 None:intergenic
ACGGGTGAGTACATCCTCAT+CGG 0.635961 3.2:-9314236 None:intergenic
CACCCGTGTGATTGTCACCA+GGG 0.641846 3.2:+9314252 MS.gene65822:CDS
TGTCCAACTTGAGCATGCAG+TGG 0.682514 3.2:+9314324 MS.gene65822:CDS
AGGATCTGATAGCTTTCACA+AGG 0.686055 3.2:+9313629 MS.gene65822:CDS
AGCCCTGGTGACAATCACAC+GGG 0.718478 3.2:-9314254 None:intergenic
GGAAACTGTTGGAACCGATG+AGG 0.724042 3.2:+9314222 MS.gene65822:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAATTGATTAATCTTAAA+AGG + chr3.2:9313746-9313765 MS.gene65822:intron 15.0%
!!! ATTCTTTTTTTAATCTTAAC+TGG - chr3.2:9313567-9313586 None:intergenic 15.0%
!!! TTTGACTTCTTAAAAAAAAA+AGG + chr3.2:9314131-9314150 MS.gene65822:intron 15.0%
!!! TTTTTTTTAAGAAGTCAAAA+TGG - chr3.2:9314130-9314149 None:intergenic 15.0%
!! TTGAACTATTTATCCTTAAT+TGG - chr3.2:9313460-9313479 None:intergenic 20.0%
!!! GGTTTTTTTTTTATGTATGT+TGG + chr3.2:9313996-9314015 MS.gene65822:intron 20.0%
!!! TTTTTGGACTTAAATTTCAA+AGG + chr3.2:9313822-9313841 MS.gene65822:intron 20.0%
! AAAATTATCTCAATCAGACT+TGG - chr3.2:9313496-9313515 None:intergenic 25.0%
! ACTTTGAGTCTATTATATGT+AGG + chr3.2:9314183-9314202 MS.gene65822:intron 25.0%
! CCATAATAAGTACTATGAAA+AGG + chr3.2:9313683-9313702 MS.gene65822:CDS 25.0%
! TGAAATATGTCTTCCAATTA+AGG + chr3.2:9313444-9313463 MS.gene65822:intron 25.0%
!! CCTTTTCATAGTACTTATTA+TGG - chr3.2:9313686-9313705 None:intergenic 25.0%
!! TTTTTATGTATGTTGGAAGA+GGG + chr3.2:9314003-9314022 MS.gene65822:intron 25.0%
!!! CTTTTTAGTAATTTGGAGTT+TGG - chr3.2:9313544-9313563 None:intergenic 25.0%
!!! TAACTGGCTTTTTAGTAATT+TGG - chr3.2:9313551-9313570 None:intergenic 25.0%
!!! TTTTTTATGTATGTTGGAAG+AGG + chr3.2:9314002-9314021 MS.gene65822:intron 25.0%
AAATTTCTCCTTACTCTGAT+GGG + chr3.2:9314373-9314392 MS.gene65822:CDS 30.0%
ACTTAAATTTCAAAGGTGGT+TGG + chr3.2:9313829-9313848 MS.gene65822:intron 30.0%
TTGGACTTAAATTTCAAAGG+TGG + chr3.2:9313825-9313844 MS.gene65822:intron 30.0%
TTTACAAGATTGTGTTGTGT+TGG + chr3.2:9313881-9313900 MS.gene65822:intron 30.0%
!!! GTAATCTCCTGAAGTTTTTT+TGG - chr3.2:9314350-9314369 None:intergenic 30.0%
AAAGCTATCAGATCCTTCAT+CGG - chr3.2:9313625-9313644 None:intergenic 35.0%
AATTTCTCCTTACTCTGATG+GGG + chr3.2:9314374-9314393 MS.gene65822:CDS 35.0%
CGTTAAGTAGCAACATACAA+AGG - chr3.2:9314051-9314070 None:intergenic 35.0%
GAAATTTCTCCTTACTCTGA+TGG + chr3.2:9314372-9314391 MS.gene65822:CDS 35.0%
!!! GTTCTTGACGTTGCTATTTT+GGG + chr3.2:9313903-9313922 MS.gene65822:intron 35.0%
!!! TTCTTGACGTTGCTATTTTG+GGG + chr3.2:9313904-9313923 MS.gene65822:intron 35.0%
AGGATCTGATAGCTTTCACA+AGG + chr3.2:9313629-9313648 MS.gene65822:CDS 40.0%
ATGGTCATTGATGCAGCTAA+TGG - chr3.2:9313667-9313686 None:intergenic 40.0%
TAGTCTAGCATTTCTTGTGC+TGG + chr3.2:9314076-9314095 MS.gene65822:intron 40.0%
TTGCAGAAATTGTCCGATGA+AGG + chr3.2:9313609-9313628 MS.gene65822:intron 40.0%
TTTGTGGCAGATTGTGATGT+TGG + chr3.2:9313968-9313987 MS.gene65822:intron 40.0%
! CAGATTGTGATGTTGGTTGA+GGG + chr3.2:9313975-9313994 MS.gene65822:intron 40.0%
!! TAGCATTTCTTGTGCTGGTT+AGG + chr3.2:9314081-9314100 MS.gene65822:intron 40.0%
!!! GGTTCTTGACGTTGCTATTT+TGG + chr3.2:9313902-9313921 MS.gene65822:intron 40.0%
!!! GTGGTGGAAGTTGTTTTTTG+TGG + chr3.2:9313952-9313971 MS.gene65822:intron 40.0%
!!! TCTTGACGTTGCTATTTTGG+GGG + chr3.2:9313905-9313924 MS.gene65822:intron 40.0%
GCAGTGGCCAAAAAAACTTC+AGG + chr3.2:9314340-9314359 MS.gene65822:CDS 45.0%
GGACTTGGAGGACATAAGAA+AGG + chr3.2:9314282-9314301 MS.gene65822:CDS 45.0%
! AAGGTGGTTGGTTGTTGTGT+TGG + chr3.2:9313841-9313860 MS.gene65822:intron 45.0%
! GCAGATTGTGATGTTGGTTG+AGG + chr3.2:9313974-9313993 MS.gene65822:intron 45.0%
! TGTATGTTGGAAGAGGGTGT+TGG + chr3.2:9314009-9314028 MS.gene65822:intron 45.0%
!! GTATGTTGGAAGAGGGTGTT+GGG + chr3.2:9314010-9314029 MS.gene65822:intron 45.0%
!!! TTTCAAATATAAATAATTTT+TGG + chr3.2:9313806-9313825 MS.gene65822:intron 5.0%
ACGGGTGAGTACATCCTCAT+CGG - chr3.2:9314239-9314258 None:intergenic 50.0%
CGCAATGCGATGGAAACTGT+TGG + chr3.2:9314211-9314230 MS.gene65822:CDS 50.0%
CTTCTCTCCCCATCAGAGTA+AGG - chr3.2:9314384-9314403 None:intergenic 50.0%
GGAAACTGTTGGAACCGATG+AGG + chr3.2:9314222-9314241 MS.gene65822:CDS 50.0%
GTAGGTTCTACGCAATGCGA+TGG + chr3.2:9314201-9314220 MS.gene65822:intron 50.0%
TGTCCAACTTGAGCATGCAG+TGG + chr3.2:9314324-9314343 MS.gene65822:CDS 50.0%
AGCCCTGGTGACAATCACAC+GGG - chr3.2:9314257-9314276 None:intergenic 55.0%
CACCCGTGTGATTGTCACCA+GGG + chr3.2:9314252-9314271 MS.gene65822:CDS 55.0%
CAGCCCTGGTGACAATCACA+CGG - chr3.2:9314258-9314277 None:intergenic 55.0%
GATTGTCACCAGGGCTGAGA+AGG + chr3.2:9314261-9314280 MS.gene65822:CDS 55.0%
TCACCCGTGTGATTGTCACC+AGG + chr3.2:9314251-9314270 MS.gene65822:CDS 55.0%
TGGCCACTGCATGCTCAAGT+TGG - chr3.2:9314330-9314349 None:intergenic 55.0%
!! GAGCAACGGATTGTAGGTGG+TGG + chr3.2:9313936-9313955 MS.gene65822:intron 55.0%
!! GTGTGCGAGCAACGGATTGT+AGG + chr3.2:9313930-9313949 MS.gene65822:intron 55.0%
!! TGCGAGCAACGGATTGTAGG+TGG + chr3.2:9313933-9313952 MS.gene65822:intron 55.0%
CACCAGGGCTGAGAAGGACT+TGG + chr3.2:9314267-9314286 MS.gene65822:CDS 60.0%
CAGGGCTGAGAAGGACTTGG+AGG + chr3.2:9314270-9314289 MS.gene65822:CDS 60.0%
CTCCAAGTCCTTCTCAGCCC+TGG - chr3.2:9314272-9314291 None:intergenic 60.0%
TGGGGGTTGTGTGCGAGCAA+CGG + chr3.2:9313922-9313941 MS.gene65822:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 9313433 9314408 9313433 ID=MS.gene65822
chr3.2 mRNA 9313433 9314408 9313433 ID=MS.gene65822.t1;Parent=MS.gene65822
chr3.2 exon 9313433 9313441 9313433 ID=MS.gene65822.t1.exon1;Parent=MS.gene65822.t1
chr3.2 CDS 9313433 9313441 9313433 ID=cds.MS.gene65822.t1;Parent=MS.gene65822.t1
chr3.2 exon 9313615 9313704 9313615 ID=MS.gene65822.t1.exon2;Parent=MS.gene65822.t1
chr3.2 CDS 9313615 9313704 9313615 ID=cds.MS.gene65822.t1;Parent=MS.gene65822.t1
chr3.2 exon 9314205 9314408 9314205 ID=MS.gene65822.t1.exon3;Parent=MS.gene65822.t1
chr3.2 CDS 9314205 9314408 9314205 ID=cds.MS.gene65822.t1;Parent=MS.gene65822.t1
Gene Sequence

>MS.gene65822

ATGACAGAGGTTGAAATATGTCTTCCAATTAAGGATAAATAGTTCAAGAGAACTCAAATTCCAAGTCTGATTGAGATAATTTTTAGTGAGACTTTACTTTATCTCTTACCAAACTCCAAATTACTAAAAAGCCAGTTAAGATTAAAAAAAGAATTTCTTGCTTGTGTGTTATTTCTTTGCAGAAATTGTCCGATGAAGGATCTGATAGCTTTCACAAGGCAGTGAGAATAGCCATTAGCTGCATCAATGACCATAATAAGTACTATGAAAAGGTGAGATTAAGAAAGAAAAACAAACACATTCATTTTTTAAACTAAATTGATTAATCTTAAAAGGACTCATATAGTAAGAGATTAATAATTTTGATTACTTTTTTCAAATATAAATAATTTTTGGACTTAAATTTCAAAGGTGGTTGGTTGTTGTGTTGGTTATTATTATTATTTTTTTTACAAGATTGTGTTGTGTTGGTTCTTGACGTTGCTATTTTGGGGGTTGTGTGCGAGCAACGGATTGTAGGTGGTGGAAGTTGTTTTTTGTGGCAGATTGTGATGTTGGTTGAGGGTTTTTTTTTTATGTATGTTGGAAGAGGGTGTTGGGTACAAATTTTGTTTTCCTTTGTATGTTGCTACTTAACGACTCATAGTCTAGCATTTCTTGTGCTGGTTAGGACTCATTGTTATTTTAATAAATTCCATTTTGACTTCTTAAAAAAAAAAGGAACAAATTGATAATTGACTCATAATTCTTACTTTGAGTCTATTATATGTAGGTTCTACGCAATGCGATGGAAACTGTTGGAACCGATGAGGATGTACTCACCCGTGTGATTGTCACCAGGGCTGAGAAGGACTTGGAGGACATAAGAAAGGTCTATTACAAGAGAAATAGTGTCCAACTTGAGCATGCAGTGGCCAAAAAAACTTCAGGAGATTACAAGAAATTTCTCCTTACTCTGATGGGGAGAGAAGAATAA

Protein sequence

>MS.gene65822.t1

MTEKLSDEGSDSFHKAVRIAISCINDHNKYYEKVLRNAMETVGTDEDVLTRVIVTRAEKDLEDIRKVYYKRNSVQLEHAVAKKTSGDYKKFLLTLMGREE