AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66532


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66532.t1 MTR_0050s0180 93.220 118 7 1 1 118 57 173 2.70e-75 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66532.t1 AT5G51720 74.390 82 19 1 37 118 28 107 2.01e-41 133

Find 34 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCACTCTTCCTTATATC+TGG 0.212325 6.2:+63183943 None:intergenic
CTGAACCATACCTGCAATAA+GGG 0.297458 6.2:+63183872 None:intergenic
ACTGAACCATACCTGCAATA+AGG 0.297703 6.2:+63183871 None:intergenic
GAGAAAGAAAATGGAATTAT+AGG 0.305770 6.2:-63184060 MS.gene66532:CDS
CGTTTAGGTGAGAAAGAAAA+TGG 0.318655 6.2:-63184069 MS.gene66532:CDS
AAAGGAACCAGTGTTGAAAT+TGG 0.358391 6.2:+63184035 None:intergenic
AACACTGGTTCCTTTGGTAT+TGG 0.361195 6.2:-63184027 MS.gene66532:CDS
TCTCACCTAAACGTGGCTTT+TGG 0.382823 6.2:+63184079 None:intergenic
AATTTCAACACTGGTTCCTT+TGG 0.387674 6.2:-63184033 MS.gene66532:CDS
TGGCTTTGTTGTGCTTTACA+TGG 0.398604 6.2:+63182535 None:intergenic
TATAAGGAAGAGTGAAGAAA+AGG 0.402584 6.2:-63183938 MS.gene66532:CDS
AGGCTGTTACCCCTTATTGC+AGG 0.413134 6.2:-63183882 MS.gene66532:intron
GTAGCATAAATCCAGATATA+AGG 0.421707 6.2:-63183954 MS.gene66532:CDS
TGATACAGATGTTGGAGATC+AGG 0.432376 6.2:-63182582 MS.gene66532:intron
TTGTGCCAAAAGCCACGTTT+AGG 0.443126 6.2:-63184084 MS.gene66532:CDS
GGCACAAAACTACACCAACT+TGG 0.466620 6.2:+63184100 None:intergenic
GTGGTGGTGAAAGCAGAGAC+AGG 0.472527 6.2:-63183985 MS.gene66532:CDS
GCAATAAGGGGTAACAGCCT+TGG 0.514083 6.2:+63183885 None:intergenic
AAGCCACTGGAGATAATGTT+GGG 0.514803 6.2:-63182518 MS.gene66532:CDS
AAAGCCACTGGAGATAATGT+TGG 0.533290 6.2:-63182519 MS.gene66532:CDS
GAAAGCAAGGAGTGTGGTGG+TGG 0.540229 6.2:-63184001 MS.gene66532:CDS
TTGGTATTGGTAAGAAAGCA+AGG 0.551760 6.2:-63184014 MS.gene66532:CDS
ACATGGCTTCCATCACATAG+AGG 0.557693 6.2:+63182552 None:intergenic
ATAGGTACCAATTTCAACAC+TGG 0.571557 6.2:-63184042 MS.gene66532:CDS
GTAAAGCACAACAAAGCCAC+TGG 0.579483 6.2:-63182531 MS.gene66532:CDS
TGCTTTCTTACCAATACCAA+AGG 0.582424 6.2:+63184017 None:intergenic
TGTGCTCAGTGAAGTCTCCA+AGG 0.596449 6.2:-63183902 MS.gene66532:CDS
TGGTGGTGAAAGCAGAGACA+GGG 0.597940 6.2:-63183984 MS.gene66532:CDS
TGGTAAGAAAGCAAGGAGTG+TGG 0.610053 6.2:-63184007 MS.gene66532:CDS
AGGCCCAACATTATCTCCAG+TGG 0.629442 6.2:+63182515 None:intergenic
TGAACCATACCTGCAATAAG+GGG 0.646965 6.2:+63183873 None:intergenic
GGTGGTGAAAGCAGAGACAG+GGG 0.666105 6.2:-63183983 MS.gene66532:CDS
GTGGTGAAAGCAGAGACAGG+GGG 0.706898 6.2:-63183982 MS.gene66532:CDS
TAAGAAAGCAAGGAGTGTGG+TGG 0.710692 6.2:-63184004 MS.gene66532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTGAAATTTGATTAT+TGG - chr6.2:63182858-63182877 MS.gene66532:intron 10.0%
!! TTGAGTAATTATAATAAAAA+GGG + chr6.2:63183855-63183874 None:intergenic 10.0%
!!! TTATGTTTTTCATTTATTTA+TGG - chr6.2:63182769-63182788 MS.gene66532:intron 10.0%
!!! TTGGAATTTTATTTTTATTA+GGG - chr6.2:63183658-63183677 MS.gene66532:intron 10.0%
!! CTTGAGTAATTATAATAAAA+AGG + chr6.2:63183856-63183875 None:intergenic 15.0%
!!! GTTGGAATTTTATTTTTATT+AGG - chr6.2:63183657-63183676 MS.gene66532:intron 15.0%
!! AAAACAAATCTTAATCATTC+TGG - chr6.2:63183997-63184016 MS.gene66532:CDS 20.0%
!! AAGGAAATTCTTACTATAAA+AGG + chr6.2:63183787-63183806 None:intergenic 20.0%
!! ATTTAGTTGAAAACAACTTA+TGG - chr6.2:63182937-63182956 MS.gene66532:intron 20.0%
!! TATCACAAATTAACAATGTT+TGG - chr6.2:63183266-63183285 MS.gene66532:intron 20.0%
!! TATGCTTCTATAATATATGT+TGG - chr6.2:63183639-63183658 MS.gene66532:intron 20.0%
!!! AGAATGATTAAGATTTGTTT+TGG + chr6.2:63183998-63184017 None:intergenic 20.0%
!!! TGATTATTGGTTAATTTATG+TGG - chr6.2:63182871-63182890 MS.gene66532:intron 20.0%
! AAGAATTTGGTACTTATTAG+TGG - chr6.2:63183601-63183620 MS.gene66532:intron 25.0%
! ACATAGACATGATTTATTTG+TGG + chr6.2:63183402-63183421 None:intergenic 25.0%
! CTAAATTCCATAAACTATCT+AGG + chr6.2:63182923-63182942 None:intergenic 25.0%
! GAGAAAGAAAATGGAATTAT+AGG - chr6.2:63182554-63182573 MS.gene66532:CDS 25.0%
! TAAGTACCAAATTCTTTATG+TGG + chr6.2:63183597-63183616 None:intergenic 25.0%
! TAGATTCCACATAAAGAATT+TGG - chr6.2:63183588-63183607 MS.gene66532:intron 25.0%
! TGATATTAGATGAATGATTC+TGG - chr6.2:63183113-63183132 MS.gene66532:intron 25.0%
!! ATGATAATCTGGAATAATGT+TGG - chr6.2:63183320-63183339 MS.gene66532:intron 25.0%
!! GCATTTTGTATATGATAATC+TGG - chr6.2:63183309-63183328 MS.gene66532:intron 25.0%
!! TGATAATCTGGAATAATGTT+GGG - chr6.2:63183321-63183340 MS.gene66532:intron 25.0%
!!! AATCTTAATCATTCTGGTTT+TGG - chr6.2:63184003-63184022 MS.gene66532:CDS 25.0%
!!! ATCTTAATCATTCTGGTTTT+GGG - chr6.2:63184004-63184023 MS.gene66532:CDS 25.0%
AAGCTATCCTAGATAGTTTA+TGG - chr6.2:63182913-63182932 MS.gene66532:intron 30.0%
ACTGGTTAAGGATACTAAAA+AGG - chr6.2:63183706-63183725 MS.gene66532:intron 30.0%
ACTTCAAAACATGAAGAACA+AGG + chr6.2:63183882-63183901 None:intergenic 30.0%
CTGGTTAAGGATACTAAAAA+GGG - chr6.2:63183707-63183726 MS.gene66532:intron 30.0%
GTAGCATAAATCCAGATATA+AGG - chr6.2:63182660-63182679 MS.gene66532:intron 30.0%
TATAAGGAAGAGTGAAGAAA+AGG - chr6.2:63182676-63182695 MS.gene66532:intron 30.0%
TATGGTTAAGTGTATCATTG+TGG - chr6.2:63182787-63182806 MS.gene66532:intron 30.0%
TGAGAAATTACGATGTTGAT+AGG - chr6.2:63183374-63183393 MS.gene66532:intron 30.0%
TGATTGATTACATCTGTTTC+TGG - chr6.2:63183192-63183211 MS.gene66532:intron 30.0%
TTTATTAGGGAAATGCTAAC+CGG - chr6.2:63183671-63183690 MS.gene66532:intron 30.0%
! GATAATCTGGAATAATGTTG+GGG - chr6.2:63183322-63183341 MS.gene66532:intron 30.0%
!! AATTCGGCAAAGTTTTATGT+TGG + chr6.2:63183500-63183519 None:intergenic 30.0%
!!! CCTTTTTTTGTATGCTTAAC+CGG - chr6.2:63183803-63183822 MS.gene66532:intron 30.0%
ATAAAAAGGGTCATGCTAAC+CGG + chr6.2:63183842-63183861 None:intergenic 35.0%
ATAGGTACCAATTTCAACAC+TGG - chr6.2:63182572-63182591 MS.gene66532:CDS 35.0%
CCGGTTAAGCATACAAAAAA+AGG + chr6.2:63183806-63183825 None:intergenic 35.0%
CGTTTAGGTGAGAAAGAAAA+TGG - chr6.2:63182545-63182564 MS.gene66532:CDS 35.0%
CTCTCAAATAGTTTCACAAG+TGG - chr6.2:63182986-63183005 MS.gene66532:intron 35.0%
TCTTCACTCTTCCTTATATC+TGG + chr6.2:63182674-63182693 None:intergenic 35.0%
TGCTTTCTTACCAATACCAA+AGG + chr6.2:63182600-63182619 None:intergenic 35.0%
TGGTCCATGTCATAGAAATT+CGG + chr6.2:63183516-63183535 None:intergenic 35.0%
TTTGCCGAATTTCTATGACA+TGG - chr6.2:63183509-63183528 MS.gene66532:intron 35.0%
! AAAGGAACCAGTGTTGAAAT+TGG + chr6.2:63182582-63182601 None:intergenic 35.0%
! AGAGGCTTGATTGTTATTGT+TGG - chr6.2:63183565-63183584 MS.gene66532:intron 35.0%
! TTTTCTTTCTCACCTAAACG+TGG + chr6.2:63182545-63182564 None:intergenic 35.0%
!! AATTTCAACACTGGTTCCTT+TGG - chr6.2:63182581-63182600 MS.gene66532:intron 35.0%
!! GAATGATTCTGGTTTCTTTC+AGG - chr6.2:63183124-63183143 MS.gene66532:intron 35.0%
!! TTAGCCTTGTTATACATTGC+CGG - chr6.2:63183421-63183440 MS.gene66532:intron 35.0%
!! TTGGTATTGGTAAGAAAGCA+AGG - chr6.2:63182600-63182619 MS.gene66532:intron 35.0%
AAAGCCACTGGAGATAATGT+TGG - chr6.2:63184095-63184114 MS.gene66532:CDS 40.0%
AAGCCACTGGAGATAATGTT+GGG - chr6.2:63184096-63184115 MS.gene66532:CDS 40.0%
ACTGAACCATACCTGCAATA+AGG + chr6.2:63182746-63182765 None:intergenic 40.0%
CAAACCCAAGTAAAAGGAGA+GGG - chr6.2:63183447-63183466 MS.gene66532:intron 40.0%
CTGAACCATACCTGCAATAA+GGG + chr6.2:63182745-63182764 None:intergenic 40.0%
TCAAACCCAAGTAAAAGGAG+AGG - chr6.2:63183446-63183465 MS.gene66532:intron 40.0%
TGAACCATACCTGCAATAAG+GGG + chr6.2:63182744-63182763 None:intergenic 40.0%
TGATACAGATGTTGGAGATC+AGG - chr6.2:63184032-63184051 MS.gene66532:CDS 40.0%
! ACAACCCTCTCCTTTTACTT+GGG + chr6.2:63183454-63183473 None:intergenic 40.0%
! GGAACTTTTCCTCTATGTGA+TGG - chr6.2:63184053-63184072 MS.gene66532:CDS 40.0%
!! AACACTGGTTCCTTTGGTAT+TGG - chr6.2:63182587-63182606 MS.gene66532:intron 40.0%
!! CTTTTACTTGGGTTTGAGAC+CGG + chr6.2:63183443-63183462 None:intergenic 40.0%
!! GCATCTGTTTTAAGCCAAGT+TGG - chr6.2:63182500-63182519 MS.gene66532:CDS 40.0%
!! GCTAGCGTTGATGTCATTTA+TGG - chr6.2:63183043-63183062 MS.gene66532:intron 40.0%
!! TGGCTTTGTTGTGCTTTACA+TGG + chr6.2:63184082-63184101 None:intergenic 40.0%
ACATGGCTTCCATCACATAG+AGG + chr6.2:63184065-63184084 None:intergenic 45.0%
CGGTCTCAAACCCAAGTAAA+AGG - chr6.2:63183441-63183460 MS.gene66532:intron 45.0%
GAGACCGGCAATGTATAACA+AGG + chr6.2:63183428-63183447 None:intergenic 45.0%
GGCACAAAACTACACCAACT+TGG + chr6.2:63182517-63182536 None:intergenic 45.0%
GGGTGCTTTGATACAGATGT+TGG - chr6.2:63184024-63184043 MS.gene66532:CDS 45.0%
GTAAAAGGAGAGGGTTGTGT+TGG - chr6.2:63183456-63183475 MS.gene66532:intron 45.0%
GTAAAGCACAACAAAGCCAC+TGG - chr6.2:63184083-63184102 MS.gene66532:CDS 45.0%
GTTACCCCTTATTGCAGGTA+TGG - chr6.2:63182737-63182756 MS.gene66532:intron 45.0%
TGGTAAGAAAGCAAGGAGTG+TGG - chr6.2:63182607-63182626 MS.gene66532:intron 45.0%
TTGTGCCAAAAGCCACGTTT+AGG - chr6.2:63182530-63182549 MS.gene66532:CDS 45.0%
! CACAACCCTCTCCTTTTACT+TGG + chr6.2:63183455-63183474 None:intergenic 45.0%
! TAAGAAAGCAAGGAGTGTGG+TGG - chr6.2:63182610-63182629 MS.gene66532:intron 45.0%
! TCTCACCTAAACGTGGCTTT+TGG + chr6.2:63182538-63182557 None:intergenic 45.0%
!! AGGGTTGTGTTGGTTGTGTT+AGG - chr6.2:63183466-63183485 MS.gene66532:intron 45.0%
ACCCTGCTTAGTGGGATAAG+AGG - chr6.2:63183547-63183566 MS.gene66532:intron 50.0%
AGCCTCTTATCCCACTAAGC+AGG + chr6.2:63183552-63183571 None:intergenic 50.0%
AGGCCCAACATTATCTCCAG+TGG + chr6.2:63184102-63184121 None:intergenic 50.0%
AGGCTGTTACCCCTTATTGC+AGG - chr6.2:63182732-63182751 MS.gene66532:intron 50.0%
GCAATAAGGGGTAACAGCCT+TGG + chr6.2:63182732-63182751 None:intergenic 50.0%
GCCTCTTATCCCACTAAGCA+GGG + chr6.2:63183551-63183570 None:intergenic 50.0%
TATCCTTAACCAGTGCCCCA+GGG + chr6.2:63183700-63183719 None:intergenic 50.0%
TGTGCTCAGTGAAGTCTCCA+AGG - chr6.2:63182712-63182731 MS.gene66532:intron 50.0%
! TGGTGGTGAAAGCAGAGACA+GGG - chr6.2:63182630-63182649 MS.gene66532:intron 50.0%
AAATGCTAACCGGTGCCCCT+GGG - chr6.2:63183681-63183700 MS.gene66532:intron 55.0%
AAGCAGGGTCAGCTCGTATG+TGG + chr6.2:63183536-63183555 None:intergenic 55.0%
ACGAGCTGACCCTGCTTAGT+GGG - chr6.2:63183539-63183558 MS.gene66532:intron 55.0%
ATCCTTAACCAGTGCCCCAG+GGG + chr6.2:63183699-63183718 None:intergenic 55.0%
GGTGGTGAAAGCAGAGACAG+GGG - chr6.2:63182631-63182650 MS.gene66532:intron 55.0%
GTATCCTTAACCAGTGCCCC+AGG + chr6.2:63183701-63183720 None:intergenic 55.0%
GTGGTGAAAGCAGAGACAGG+GGG - chr6.2:63182632-63182651 MS.gene66532:intron 55.0%
TACGAGCTGACCCTGCTTAG+TGG - chr6.2:63183538-63183557 MS.gene66532:intron 55.0%
TGTATGCTTAACCGGTGCCC+CGG - chr6.2:63183811-63183830 MS.gene66532:intron 55.0%
! GAAAGCAAGGAGTGTGGTGG+TGG - chr6.2:63182613-63182632 MS.gene66532:intron 55.0%
! GTGGTGGTGAAAGCAGAGAC+AGG - chr6.2:63182629-63182648 MS.gene66532:intron 55.0%
AATGCTAACCGGTGCCCCTG+GGG - chr6.2:63183682-63183701 MS.gene66532:intron 60.0%
GAAATGCTAACCGGTGCCCC+TGG - chr6.2:63183680-63183699 MS.gene66532:intron 60.0%
GTATGCTTAACCGGTGCCCC+GGG - chr6.2:63183812-63183831 MS.gene66532:intron 60.0%
GTCATGCTAACCGGTGTCCC+CGG + chr6.2:63183833-63183852 None:intergenic 60.0%
TATGCTTAACCGGTGCCCCG+GGG - chr6.2:63183813-63183832 MS.gene66532:intron 60.0%
TCATGCTAACCGGTGTCCCC+GGG + chr6.2:63183832-63183851 None:intergenic 60.0%
CATGCTAACCGGTGTCCCCG+GGG + chr6.2:63183831-63183850 None:intergenic 65.0%
!! TGCCCCTGGGGCACTGGTTA+AGG - chr6.2:63183694-63183713 MS.gene66532:intron 65.0%
! AACCAGTGCCCCAGGGGCAC+CGG + chr6.2:63183693-63183712 None:intergenic 70.0%
AACCGGTGCCCCGGGGACAC+CGG - chr6.2:63183820-63183839 MS.gene66532:intron 75.0%
AACCGGTGCCCCTGGGGCAC+TGG - chr6.2:63183688-63183707 MS.gene66532:intron 75.0%
AACCGGTGTCCCCGGGGCAC+CGG + chr6.2:63183825-63183844 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 63182497 63184139 63182497 ID=MS.gene66532
chr6.2 mRNA 63182497 63184139 63182497 ID=MS.gene66532.t1;Parent=MS.gene66532
chr6.2 exon 63183883 63184139 63183883 ID=MS.gene66532.t1.exon1;Parent=MS.gene66532.t1
chr6.2 CDS 63183883 63184139 63183883 ID=cds.MS.gene66532.t1;Parent=MS.gene66532.t1
chr6.2 exon 63182497 63182596 63182497 ID=MS.gene66532.t1.exon2;Parent=MS.gene66532.t1
chr6.2 CDS 63182497 63182596 63182497 ID=cds.MS.gene66532.t1;Parent=MS.gene66532.t1
Gene Sequence

>MS.gene66532

ATGGCATCTGTTTTAAGCCAAGTTGGTGTAGTTTTGTGCCAAAAGCCACGTTTAGGTGAGAAAGAAAATGGAATTATAGGTACCAATTTCAACACTGGTTCCTTTGGTATTGGTAAGAAAGCAAGGAGTGTGGTGGTGGTGAAAGCAGAGACAGGGGGTGTGAGTAGCATAAATCCAGATATAAGGAAGAGTGAAGAAAAGGTTGTTGATGCTGTTGTGCTCAGTGAAGTCTCCAAGGCTGTTACCCCTTATTGCAGGTATGGTTCAGTTTTTTATGTTTTTCATTTATTTATGGTTAAGTGTATCATTGTGGCTCTTACTTTATGTGATTTTAGTATGTTAAATTTTTTAAATCTTGATCTATTATTGAAATTTGATTATTGGTTAATTTATGTGGTTTTAGTATCTTATTAGTGAAGCTATCCTAGATAGTTTATGGAATTTAGTTGAAAACAACTTATGGACAAGTCGTAGCTGTTTTCATAAGTTCTCTCAAATAGTTTCACAAGTGGCTATGTCGCACAAAGATCGAATCGAAAATCAATAGCTAGCGTTGATGTCATTTATGGTTAAGATCAAAATTATGAAACTCTAGTTTGTTATATTAGCATTTGCTTGATATTAGATGAATGATTCTGGTTTCTTTCAGGCTTGAAAACTAGTTAAAGAATATACATTTCGTATCAAACTATGATTGATTGATTACATCTGTTTCTGGAAGTGATTAAAAAAAATGTCATTTGTGATGTTAATTTCTTTTCAGCTATTTTATCACAAATTAACAATGTTTGGTTAAATTGTATTAGTTGAATGCATTTTGTATATGATAATCTGGAATAATGTTGGGGTAGCATAAAAAAGAGAGTGAATCTCTTGTTGAGAAATTACGATGTTGATAGGTTCCACAAATAAATCATGTCTATGTTAGCCTTGTTATACATTGCCGGTCTCAAACCCAAGTAAAAGGAGAGGGTTGTGTTGGTTGTGTTAGGCTCGACAGCCAACATAAAACTTTGCCGAATTTCTATGACATGGACCACATACGAGCTGACCCTGCTTAGTGGGATAAGAGGCTTGATTGTTATTGTTGGTAGATTCCACATAAAGAATTTGGTACTTATTAGTGGAAAGTTTCTTTATCATATGCTTCTATAATATATGTTGGAATTTTATTTTTATTAGGGAAATGCTAACCGGTGCCCCTGGGGCACTGGTTAAGGATACTAAAAAGGGAAATAATATAGTAATTATTGCATTGAAAAATGTGTAATTAATTCAAAAAAGTTTCCTTTTATAGTAAGAATTTCCTTTTTTTGTATGCTTAACCGGTGCCCCGGGGACACCGGTTAGCATGACCCTTTTTATTATAATTACTCAAGAAACCTTGTTCTTCATGTTTTGAAGTTATGATCTTAGCAACAGTTAACGATGACATGAAAAGCAACATTTACATATTCTTTCTGCAATCTTCTTGAATTTTAGTTTTCATGTTTAAATTCCAAAACAAATCTTAATCATTCTGGTTTTGGGTGCTTTGATACAGATGTTGGAGATCAGGAACTTTTCCTCTATGTGATGGAAGCCATGTAAAGCACAACAAAGCCACTGGAGATAATGTTGGGCCTCTTCTTTTGAAAAAATAG

Protein sequence

>MS.gene66532.t1

MASVLSQVGVVLCQKPRLGEKENGIIGTNFNTGSFGIGKKARSVVVVKAETGGVSSINPDIRKSEEKVVDAVVLSEVSKAVTPYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKK