AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053181


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053181.t1 MTR_4g007600 63.536 181 28 4 1 178 1 146 2.66e-32 114
MS.gene053181.t1 MTR_4g007620 66.234 77 24 2 1 76 13 88 1.18e-22 89.4
MS.gene053181.t1 MTR_4g007590 52.326 86 31 2 1 76 1 86 6.46e-19 79.0
MS.gene053181.t1 MTR_4g007570 55.263 76 28 2 1 76 1 70 9.33e-19 78.2
MS.gene053181.t1 MTR_4g007560 46.154 117 29 2 1 110 1 90 2.04e-14 67.8
MS.gene053181.t1 MTR_4g007580 52.000 75 25 4 1 74 1 65 2.22e-12 61.6
MS.gene053181.t1 MTR_4g007570 40.000 110 34 3 1 110 1 78 3.63e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAGGTGGAAGGGGAGG+AGG 0.294260 4.2:+92218160 MS.gene053181:CDS
ACGATAATAGGAAATTTATA+TGG 0.302449 4.2:+92218031 MS.gene053181:intron
GCAAGCGGAGGAGGTGGATT+TGG 0.303229 4.2:+92218199 MS.gene053181:CDS
GGAGGTGGAAGGGGAGGAGG+TGG 0.304793 4.2:+92218163 MS.gene053181:CDS
GGTGGAGGAAAGGGAGGAGG+TGG 0.314138 4.2:+92218268 MS.gene053181:CDS
GCCTTCATCTTCATTTGAAA+TGG 0.326206 4.2:-92217875 None:intergenic
AGGGGAGGAGGTGGATTTAG+TGG 0.341806 4.2:+92218172 MS.gene053181:CDS
GGTGGAGGAAAGGGAGGAGG+TGG 0.346499 4.2:+92218223 MS.gene053181:CDS
GGAAAGGGAGGAGGTGGAAT+GGG 0.349740 4.2:+92218274 MS.gene053181:CDS
GGAATAGGAGGTGGAGGAAA+GGG 0.351372 4.2:+92218259 MS.gene053181:CDS
GGATTTGGAGGAGGAGTAGC+AGG 0.351628 4.2:+92218310 MS.gene053181:CDS
AAGGGAGGAGGTGGAATGGG+AGG 0.356510 4.2:+92218277 MS.gene053181:CDS
GAACAAGCACATGAGGTTAA+TGG 0.363274 4.2:+92217919 MS.gene053181:CDS
ATAGGAGGTGGAGGAAAGGG+AGG 0.364127 4.2:+92218262 MS.gene053181:CDS
GGATTTGGAGGTGGAGGAAA+GGG 0.386379 4.2:+92218214 MS.gene053181:CDS
TGGAATAGGAGGTGGAGGAA+AGG 0.387806 4.2:+92218258 MS.gene053181:CDS
GGAGGTGGAGGAAAGGGAGG+AGG 0.395444 4.2:+92218265 MS.gene053181:CDS
TTTGGAGGTGGAGGAAAGGG+AGG 0.396785 4.2:+92218217 MS.gene053181:CDS
GGAGGAGGTGGAATGGGAGG+GGG 0.398655 4.2:+92218280 MS.gene053181:CDS
GGATCTCGAGGAGGTGGAAT+AGG 0.402755 4.2:+92218244 MS.gene053181:CDS
TCGACGGGAGGAGGTGGAAG+GGG 0.410147 4.2:+92218154 MS.gene053181:CDS
CATGTGCTTGTTCATTACTC+TGG 0.410300 4.2:-92217909 None:intergenic
ATAGGAAATTTATATGGAAA+TGG 0.411309 4.2:+92218037 MS.gene053181:intron
GAGCATTTGAGATGATCAAT+AGG 0.414061 4.2:-92217849 None:intergenic
AGGAAAGGGAGGAGGTGGAA+TGG 0.417507 4.2:+92218273 MS.gene053181:CDS
GGAGCGGCCGGTGGAATTAT+CGG 0.418438 4.2:+92218334 MS.gene053181:CDS
GGAGGTGGAGGAAAGGGAGG+AGG 0.423397 4.2:+92218220 MS.gene053181:CDS
ACGGGAGGAGGTGGAAGGGG+AGG 0.424441 4.2:+92218157 MS.gene053181:CDS
TGGATTTGGAGGTGGAGGAA+AGG 0.425116 4.2:+92218213 MS.gene053181:CDS
GGTGGGAAGGGATTTGGAGG+AGG 0.425225 4.2:+92218301 MS.gene053181:CDS
GGAGTAGCAGGTGGAGCGGC+CGG 0.432536 4.2:+92218322 MS.gene053181:CDS
GGGAGGAGGTGGAATGGGAG+GGG 0.432996 4.2:+92218279 MS.gene053181:CDS
ATTATCGGTGGTGGAATTGT+TGG 0.456986 4.2:+92218349 MS.gene053181:CDS
GGAGGTGGATCTCGAGGAGG+TGG 0.481043 4.2:+92218238 MS.gene053181:CDS
AGGGAGGAGGTGGAATGGGA+GGG 0.484959 4.2:+92218278 MS.gene053181:CDS
AGTGGAGGTGCAAGCGGAGG+AGG 0.488977 4.2:+92218190 MS.gene053181:CDS
GCCATTTCAAATGAAGATGA+AGG 0.496545 4.2:+92217874 MS.gene053181:CDS
GCCGGTGGAATTATCGGTGG+TGG 0.507892 4.2:+92218340 MS.gene053181:CDS
GGAGGTGCAAGCGGAGGAGG+TGG 0.508022 4.2:+92218193 MS.gene053181:CDS
GGAGGTGGAATAGGAGGTGG+AGG 0.508513 4.2:+92218253 MS.gene053181:CDS
ATCGGTGGTGGAATTGTTGG+CGG 0.516356 4.2:+92218352 MS.gene053181:CDS
TTTCGACGGGAGGAGGTGGA+AGG 0.517005 4.2:+92218152 MS.gene053181:CDS
AGCGGAGGAGGTGGATTTGG+AGG 0.527084 4.2:+92218202 MS.gene053181:CDS
CGAGGAGGTGGAATAGGAGG+TGG 0.535172 4.2:+92218250 MS.gene053181:CDS
GGAGGTGGATTTGGAGGTGG+AGG 0.536832 4.2:+92218208 MS.gene053181:CDS
GCAAATCGCGGTCACAATGG+TGG 0.542074 4.2:+92218382 MS.gene053181:CDS
TTCGACGGGAGGAGGTGGAA+GGG 0.547737 4.2:+92218153 MS.gene053181:CDS
TCTCGAGGAGGTGGAATAGG+AGG 0.549780 4.2:+92218247 MS.gene053181:CDS
AAGGGAGGAGGTGGATCTCG+AGG 0.551250 4.2:+92218232 MS.gene053181:CDS
GTAGCAGGTGGAGCGGCCGG+TGG 0.556374 4.2:+92218325 MS.gene053181:CDS
GGCGGCGCGGTTGCAAATCG+CGG 0.557081 4.2:+92218370 MS.gene053181:CDS
GGAGGAGGTGGATTTGGAGG+TGG 0.558096 4.2:+92218205 MS.gene053181:CDS
AGGAGGAGTAGCAGGTGGAG+CGG 0.582710 4.2:+92218318 MS.gene053181:CDS
CAAATCGCGGTCACAATGGT+GGG 0.586802 4.2:+92218383 MS.gene053181:CDS
TCCTCCCGTCGAAAACCTCA+TGG 0.608672 4.2:-92218143 None:intergenic
CAATGTTGAGACACAAAATG+AGG 0.609760 4.2:-92218448 None:intergenic
GAGTAATGAACAAGCACATG+AGG 0.616284 4.2:+92217912 MS.gene053181:CDS
GGAGGAGGTGGATTTAGTGG+AGG 0.619290 4.2:+92218175 MS.gene053181:CDS
GGATTTAGTGGAGGTGCAAG+CGG 0.621029 4.2:+92218184 MS.gene053181:CDS
TCCACCACCGATAATTCCAC+CGG 0.625861 4.2:-92218341 None:intergenic
TGGTGGAATTGTTGGCGGCG+CGG 0.634398 4.2:+92218357 MS.gene053181:CDS
GGTCACAATGGTGGGTACAA+TGG 0.639906 4.2:+92218391 MS.gene053181:CDS
TTTAGTGGAGGTGCAAGCGG+AGG 0.644874 4.2:+92218187 MS.gene053181:CDS
GTTGCAAATCGCGGTCACAA+TGG 0.657933 4.2:+92218379 MS.gene053181:CDS
TTTGGAGGAGGAGTAGCAGG+TGG 0.662305 4.2:+92218313 MS.gene053181:CDS
GGAGGAGGTGGATCTCGAGG+AGG 0.673141 4.2:+92218235 MS.gene053181:CDS
GCGGCCGGTGGAATTATCGG+TGG 0.683638 4.2:+92218337 MS.gene053181:CDS
GTAACATAGAAGTCTCCACA+AGG 0.724906 4.2:+92218059 MS.gene053181:CDS
ATGAATTGTCTGTTGCCATG+AGG 0.730410 4.2:+92218128 MS.gene053181:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACGATAATAGGAAATTTATA+TGG + chr4.2:92218031-92218050 MS.gene053181:intron 20.0%
!! ATAGGAAATTTATATGGAAA+TGG + chr4.2:92218037-92218056 MS.gene053181:intron 20.0%
!!! AAGTTTTTACTTGTTTGAAA+AGG - chr4.2:92217958-92217977 None:intergenic 20.0%
! TTTACTTGTTTGAAAAGGTA+AGG - chr4.2:92217953-92217972 None:intergenic 25.0%
!! TTGTGTTATTCAACGATAAT+AGG + chr4.2:92218019-92218038 MS.gene053181:intron 25.0%
GAACAACAAAAATGTGATTG+TGG + chr4.2:92218084-92218103 MS.gene053181:CDS 30.0%
!!! ATTTTTGTTGTTCAACCTTG+TGG - chr4.2:92218077-92218096 None:intergenic 30.0%
CAATGTTGAGACACAAAATG+AGG - chr4.2:92218451-92218470 None:intergenic 35.0%
GCCATTTCAAATGAAGATGA+AGG + chr4.2:92217874-92217893 MS.gene053181:CDS 35.0%
GCCTTCATCTTCATTTGAAA+TGG - chr4.2:92217878-92217897 None:intergenic 35.0%
! GAGCATTTGAGATGATCAAT+AGG - chr4.2:92217852-92217871 None:intergenic 35.0%
ATGAATTGTCTGTTGCCATG+AGG + chr4.2:92218128-92218147 MS.gene053181:CDS 40.0%
CATGTGCTTGTTCATTACTC+TGG - chr4.2:92217912-92217931 None:intergenic 40.0%
GAACAAGCACATGAGGTTAA+TGG + chr4.2:92217919-92217938 MS.gene053181:CDS 40.0%
GAGTAATGAACAAGCACATG+AGG + chr4.2:92217912-92217931 MS.gene053181:CDS 40.0%
GTAACATAGAAGTCTCCACA+AGG + chr4.2:92218059-92218078 MS.gene053181:CDS 40.0%
!! ATTATCGGTGGTGGAATTGT+TGG + chr4.2:92218349-92218368 MS.gene053181:CDS 40.0%
! TGTTGCCATGAGGTTTTCGA+CGG + chr4.2:92218138-92218157 MS.gene053181:CDS 45.0%
CAAATCGCGGTCACAATGGT+GGG + chr4.2:92218383-92218402 MS.gene053181:CDS 50.0%
GGAATAGGAGGTGGAGGAAA+GGG + chr4.2:92218259-92218278 MS.gene053181:CDS 50.0%
GGATTTGGAGGTGGAGGAAA+GGG + chr4.2:92218214-92218233 MS.gene053181:CDS 50.0%
GTTGCAAATCGCGGTCACAA+TGG + chr4.2:92218379-92218398 MS.gene053181:CDS 50.0%
TCCACCACCGATAATTCCAC+CGG - chr4.2:92218344-92218363 None:intergenic 50.0%
TGGAATAGGAGGTGGAGGAA+AGG + chr4.2:92218258-92218277 MS.gene053181:CDS 50.0%
TGGATTTGGAGGTGGAGGAA+AGG + chr4.2:92218213-92218232 MS.gene053181:CDS 50.0%
! GGATTTAGTGGAGGTGCAAG+CGG + chr4.2:92218184-92218203 MS.gene053181:CDS 50.0%
! GTTGCCATGAGGTTTTCGAC+GGG + chr4.2:92218139-92218158 MS.gene053181:CDS 50.0%
!! ATCGGTGGTGGAATTGTTGG+CGG + chr4.2:92218352-92218371 MS.gene053181:CDS 50.0%
!! GGTCACAATGGTGGGTACAA+TGG + chr4.2:92218391-92218410 MS.gene053181:CDS 50.0%
AGGAAAGGGAGGAGGTGGAA+TGG + chr4.2:92218273-92218292 MS.gene053181:CDS 55.0%
ATAGGAGGTGGAGGAAAGGG+AGG + chr4.2:92218262-92218281 MS.gene053181:CDS 55.0%
GCAAATCGCGGTCACAATGG+TGG + chr4.2:92218382-92218401 MS.gene053181:CDS 55.0%
GGAAAGGGAGGAGGTGGAAT+GGG + chr4.2:92218274-92218293 MS.gene053181:CDS 55.0%
GGATCTCGAGGAGGTGGAAT+AGG + chr4.2:92218244-92218263 MS.gene053181:CDS 55.0%
GGATTTGGAGGAGGAGTAGC+AGG + chr4.2:92218310-92218329 MS.gene053181:CDS 55.0%
TCCTCCCGTCGAAAACCTCA+TGG - chr4.2:92218146-92218165 None:intergenic 55.0%
TCTCGAGGAGGTGGAATAGG+AGG + chr4.2:92218247-92218266 MS.gene053181:CDS 55.0%
TTTGGAGGAGGAGTAGCAGG+TGG + chr4.2:92218313-92218332 MS.gene053181:CDS 55.0%
TTTGGAGGTGGAGGAAAGGG+AGG + chr4.2:92218217-92218236 MS.gene053181:CDS 55.0%
! AGGGGAGGAGGTGGATTTAG+TGG + chr4.2:92218172-92218191 MS.gene053181:CDS 55.0%
! ATGAGGTTTTCGACGGGAGG+AGG + chr4.2:92218145-92218164 MS.gene053181:CDS 55.0%
! GGAGGAGGTGGATTTAGTGG+AGG + chr4.2:92218175-92218194 MS.gene053181:CDS 55.0%
! TTTAGTGGAGGTGCAAGCGG+AGG + chr4.2:92218187-92218206 MS.gene053181:CDS 55.0%
AAGGGAGGAGGTGGAATGGG+AGG + chr4.2:92218277-92218296 MS.gene053181:CDS 60.0%
AAGGGAGGAGGTGGATCTCG+AGG + chr4.2:92218232-92218251 MS.gene053181:CDS 60.0%
AGGAGGAGTAGCAGGTGGAG+CGG + chr4.2:92218318-92218337 MS.gene053181:CDS 60.0%
AGGGAGGAGGTGGAATGGGA+GGG + chr4.2:92218278-92218297 MS.gene053181:CDS 60.0%
CGAGGAGGTGGAATAGGAGG+TGG + chr4.2:92218250-92218269 MS.gene053181:CDS 60.0%
GCCGGTGGAATTATCGGTGG+TGG + chr4.2:92218340-92218359 MS.gene053181:CDS 60.0%
GGAGCGGCCGGTGGAATTAT+CGG + chr4.2:92218334-92218353 MS.gene053181:CDS 60.0%
GGAGGTGGAATAGGAGGTGG+AGG + chr4.2:92218253-92218272 MS.gene053181:CDS 60.0%
GGTGGGAAGGGATTTGGAGG+AGG + chr4.2:92218301-92218320 MS.gene053181:CDS 60.0%
TGGTGGAATTGTTGGCGGCG+CGG + chr4.2:92218357-92218376 MS.gene053181:CDS 60.0%
TTCGACGGGAGGAGGTGGAA+GGG + chr4.2:92218153-92218172 MS.gene053181:CDS 60.0%
TTTCGACGGGAGGAGGTGGA+AGG + chr4.2:92218152-92218171 MS.gene053181:CDS 60.0%
! AGCGGAGGAGGTGGATTTGG+AGG + chr4.2:92218202-92218221 MS.gene053181:CDS 60.0%
! AGGTTTTCGACGGGAGGAGG+TGG + chr4.2:92218148-92218167 MS.gene053181:CDS 60.0%
! GCAAGCGGAGGAGGTGGATT+TGG + chr4.2:92218199-92218218 MS.gene053181:CDS 60.0%
! GCCATGAGGTTTTCGACGGG+AGG + chr4.2:92218142-92218161 MS.gene053181:CDS 60.0%
! GGAGGAGGTGGATTTGGAGG+TGG + chr4.2:92218205-92218224 MS.gene053181:CDS 60.0%
! GGAGGTGGATTTGGAGGTGG+AGG + chr4.2:92218208-92218227 MS.gene053181:CDS 60.0%
GAGGTGGAATGGGAGGGGGT+GGG + chr4.2:92218284-92218303 MS.gene053181:CDS 65.0%
GCGGCCGGTGGAATTATCGG+TGG + chr4.2:92218337-92218356 MS.gene053181:CDS 65.0%
GGAATGGGAGGGGGTGGGAA+GGG + chr4.2:92218289-92218308 MS.gene053181:CDS 65.0%
GGAGGAGGTGGAATGGGAGG+GGG + chr4.2:92218280-92218299 MS.gene053181:CDS 65.0%
GGAGGAGGTGGATCTCGAGG+AGG + chr4.2:92218235-92218254 MS.gene053181:CDS 65.0%
GGAGGGGGTGGGAAGGGATT+TGG + chr4.2:92218295-92218314 MS.gene053181:CDS 65.0%
GGAGGTGGAGGAAAGGGAGG+AGG + chr4.2:92218220-92218239 MS.gene053181:CDS 65.0%
GGAGGTGGATCTCGAGGAGG+TGG + chr4.2:92218238-92218257 MS.gene053181:CDS 65.0%
GGGAGGAGGTGGAATGGGAG+GGG + chr4.2:92218279-92218298 MS.gene053181:CDS 65.0%
GGGGGTGGGAAGGGATTTGG+AGG + chr4.2:92218298-92218317 MS.gene053181:CDS 65.0%
GGTGGAGGAAAGGGAGGAGG+TGG + chr4.2:92218223-92218242 MS.gene053181:CDS 65.0%
TCGACGGGAGGAGGTGGAAG+GGG + chr4.2:92218154-92218173 MS.gene053181:CDS 65.0%
TGGAATGGGAGGGGGTGGGA+AGG + chr4.2:92218288-92218307 MS.gene053181:CDS 65.0%
! AGTGGAGGTGCAAGCGGAGG+AGG + chr4.2:92218190-92218209 MS.gene053181:CDS 65.0%
ACGGGAGGAGGTGGAAGGGG+AGG + chr4.2:92218157-92218176 MS.gene053181:CDS 70.0%
GGAGGAGGTGGAAGGGGAGG+AGG + chr4.2:92218160-92218179 MS.gene053181:CDS 70.0%
GGAGGTGGAAGGGGAGGAGG+TGG + chr4.2:92218163-92218182 MS.gene053181:CDS 70.0%
GGAGGTGGAATGGGAGGGGG+TGG + chr4.2:92218283-92218302 MS.gene053181:CDS 70.0%
GGAGTAGCAGGTGGAGCGGC+CGG + chr4.2:92218322-92218341 MS.gene053181:CDS 70.0%
GGCGGCGCGGTTGCAAATCG+CGG + chr4.2:92218370-92218389 MS.gene053181:CDS 70.0%
! GGAGGTGCAAGCGGAGGAGG+TGG + chr4.2:92218193-92218212 MS.gene053181:CDS 70.0%
GTAGCAGGTGGAGCGGCCGG+TGG + chr4.2:92218325-92218344 MS.gene053181:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 92217823 92218471 92217823 ID=MS.gene053181
chr4.2 mRNA 92217823 92218471 92217823 ID=MS.gene053181.t1;Parent=MS.gene053181
chr4.2 exon 92217823 92217940 92217823 ID=MS.gene053181.t1.exon1;Parent=MS.gene053181.t1
chr4.2 CDS 92217823 92217940 92217823 ID=cds.MS.gene053181.t1;Parent=MS.gene053181.t1
chr4.2 exon 92218041 92218471 92218041 ID=MS.gene053181.t1.exon2;Parent=MS.gene053181.t1
chr4.2 CDS 92218041 92218471 92218041 ID=cds.MS.gene053181.t1;Parent=MS.gene053181.t1
Gene Sequence

>MS.gene053181

ATGAAGGTAATATTTTTTGTATTGTTCCTATTGATCATCTCAAATGCTCAAGCCATTTCAAATGAAGATGAAGGCAAAAACATAATCCAGAGTAATGAACAAGCACATGAGGTTAATGGTACAATTTCCTTACCTTTTCAAACAAGTAAAAACTTAGTTAACGTTTCACACATCTGTGAGCATACGTGTTTTATAATTGTGTTATTCAACGATAATAGGAAATTTATATGGAAATGGTAACATAGAAGTCTCCACAAGGTTGAACAACAAAAATGTGATTGTGGCAAGTAAAAATGAAGATAAACATGAATTGTCTGTTGCCATGAGGTTTTCGACGGGAGGAGGTGGAAGGGGAGGAGGTGGATTTAGTGGAGGTGCAAGCGGAGGAGGTGGATTTGGAGGTGGAGGAAAGGGAGGAGGTGGATCTCGAGGAGGTGGAATAGGAGGTGGAGGAAAGGGAGGAGGTGGAATGGGAGGGGGTGGGAAGGGATTTGGAGGAGGAGTAGCAGGTGGAGCGGCCGGTGGAATTATCGGTGGTGGAATTGTTGGCGGCGCGGTTGCAAATCGCGGTCACAATGGTGGGTACAATGGCACTCATAATTCTGCAACAACATTATCTGCAGTGCCTCATTTTGTGTCTCAACATTGA

Protein sequence

>MS.gene053181.t1

MKVIFFVLFLLIISNAQAISNEDEGKNIIQSNEQAHEVNGNLYGNGNIEVSTRLNNKNVIVASKNEDKHELSVAMRFSTGGGGRGGGGFSGGASGGGGFGGGGKGGGGSRGGGIGGGGKGGGGMGGGGKGFGGGVAGGAAGGIIGGGIVGGAVANRGHNGGYNGTHNSATTLSAVPHFVSQH