AlfalfaGEDB | MS.gene74072

M. sativa cultivar XinJiangDaYe / MS.gene74072

KEGG: K22588
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0008168 GO:0008171 GO:0016740 GO:0016741 GO:0046983
PFAM: PF00891.21 PF08100.14

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74072.t1	XP_003621878.2	92.3	350	27	0	1	350	1	350	3.10E-186	661

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74072.t1	O22308	55.4	350	149	3	3	348	4	350	3.8e-108	392.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74072.t1	G7KWV7	92.3	350	27	0	1	350	1	350	2.2e-186	661.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049281	MS.gene74072	0.815295	9.95E-52	-1.69E-46
MS.gene049611	MS.gene74072	0.812421	4.28E-51	-1.69E-46
MS.gene050216	MS.gene74072	-0.813976	1.95E-51	-1.69E-46
MS.gene050381	MS.gene74072	0.839289	1.73E-57	-1.69E-46
MS.gene050496	MS.gene74072	-0.824382	8.32E-54	-1.69E-46
MS.gene050504	MS.gene74072	-0.80519	1.50E-49	-1.69E-46
MS.gene050612	MS.gene74072	-0.810409	1.17E-50	-1.69E-46
MS.gene050868	MS.gene74072	-0.804971	1.67E-49	-1.69E-46
MS.gene050871	MS.gene74072	-0.805814	1.11E-49	-1.69E-46
MS.gene050883	MS.gene74072	0.824957	6.09E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74072.t1	MTR_7g024500	92.286	350	27	0	1	350	1	350	0.0	677
MS.gene74072.t1	MTR_7g024520	86.286	350	48	0	1	350	1	350	0.0	632
MS.gene74072.t1	MTR_4g088345	64.407	354	120	2	1	348	1	354	3.61e-172	483
MS.gene74072.t1	MTR_4g088340	64.350	331	113	1	23	348	1	331	1.60e-159	450
MS.gene74072.t1	MTR_7g014570	55.714	350	148	3	3	348	4	350	3.55e-139	399
MS.gene74072.t1	MTR_1g076940	54.494	356	150	5	3	347	4	358	1.46e-138	398
MS.gene74072.t1	MTR_7g014510	52.825	354	159	3	2	348	3	355	9.94e-133	383
MS.gene74072.t1	MTR_4g088200	46.893	354	178	4	5	348	6	359	4.64e-113	333
MS.gene74072.t1	MTR_4g033150	48.603	358	172	4	3	348	4	361	3.87e-112	331
MS.gene74072.t1	MTR_5g074680	46.264	348	179	4	9	348	11	358	2.41e-111	328

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74072.t1	AT4G35150	35.735	347	171	10	14	346	12	320	1.42e-57	190
MS.gene74072.t1	AT4G35160	36.490	359	195	13	14	346	26	377	4.80e-53	179
MS.gene74072.t1	AT5G54160	32.530	332	207	9	29	348	33	359	7.57e-49	168
MS.gene74072.t1	AT1G51990	32.857	280	162	8	85	348	91	360	9.38e-41	147
MS.gene74072.t1	AT1G51990	32.857	280	162	8	85	348	91	360	1.09e-40	147
MS.gene74072.t1	AT3G53140	30.233	344	204	11	29	348	26	357	4.42e-35	131
MS.gene74072.t1	AT1G33030	29.430	316	206	8	29	331	20	331	5.01e-35	131
MS.gene74072.t1	AT1G77520	25.581	344	225	10	29	348	43	379	2.50e-31	122
MS.gene74072.t1	AT1G63140	28.125	352	218	11	23	348	37	379	1.02e-28	115
MS.gene74072.t1	AT1G63140	28.125	352	218	11	23	348	57	399	1.54e-28	114

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Find 54 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGTGGTAGAAGGATTAA+CGG	0.461848	7.4:+76833674	MS.gene74072:CDS
AACAGCCCACTTGATCGACG+CGG	0.727407	7.4:-76833106	None:intergenic
AAGAAAATCATAACAGAAGC+AGG	0.478707	7.4:+76834445	MS.gene74072:CDS
AATATTATCAATTGAGCAAA+TGG	0.394667	7.4:+76833400	MS.gene74072:CDS
AATATTTATCAACCATGGTA+GGG	0.522593	7.4:-76833383	None:intergenic
AATCTTCGCAATTGTCAAGA+TGG	0.505388	7.4:+76833263	MS.gene74072:CDS
AATCTTTGAGACTACATTAT+GGG	0.349412	7.4:+76833443	MS.gene74072:CDS
AATGTGGTAGAAGGATTAAC+GGG	0.469980	7.4:+76833675	MS.gene74072:CDS
AATTGCATCAGCTTGAGGGA+TGG	0.574477	7.4:-76833739	None:intergenic
ACAAGCTCAGGAAGAGTAAT+AGG	0.291026	7.4:-76833168	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGTGGTAGAAGGATTAA+CGG	+	chr7.4:76833674-76833693	MS.gene74072:CDS	30.0%
AAGAAAATCATAACAGAAGC+AGG	+	chr7.4:76834445-76834464	MS.gene74072:CDS	30.0%
AGAGTGATTCTAATATGATG+AGG	+	chr7.4:76833517-76833536	MS.gene74072:CDS	30.0%
AGTGACCTAAAACCAAATAT+AGG	-	chr7.4:76834493-76834512	None:intergenic	30.0%
ATAGATTCGCAATTAGGTAA+AGG	-	chr7.4:76833815-76833834	None:intergenic	30.0%
CAAGGTACGTATTAATTCAA+AGG	+	chr7.4:76833767-76833786	MS.gene74072:intron	30.0%
CACGGTTAAAATTATTTGTG+AGG	+	chr7.4:76833614-76833633	MS.gene74072:CDS	30.0%
CCAGATATAATCCAAAATCA+TGG	+	chr7.4:76833141-76833160	MS.gene74072:CDS	30.0%
CTAATTGCGAATCTATATGT+CGG	+	chr7.4:76833819-76833838	MS.gene74072:intron	30.0%
CTGCTTTGATATAACTATGA+TGG	+	chr7.4:76834384-76834403	MS.gene74072:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	76833021	76834531	76833021	ID=MS.gene74072
chr7.4	mRNA	76833021	76834531	76833021	ID=MS.gene74072.t1;Parent=MS.gene74072
chr7.4	exon	76833021	76833770	76833021	ID=MS.gene74072.t1.exon1;Parent=MS.gene74072.t1
chr7.4	CDS	76833021	76833770	76833021	ID=cds.MS.gene74072.t1;Parent=MS.gene74072.t1
chr7.4	exon	76834229	76834531	76834229	ID=MS.gene74072.t1.exon2;Parent=MS.gene74072.t1
chr7.4	CDS	76834229	76834531	76834229	ID=cds.MS.gene74072.t1;Parent=MS.gene74072.t1

Gene Sequence

>MS.gene74072

ATGGGTTCTGAGAATGATTGCAAAGCAAGTGAGCTCTTTGAAGCTCAAGCTCACTTGTACAATCAGTTATTCAGTTTCATGAGACCCGCGTCGATCAAGTGGGCTGTTGAACTTGGCATACCAGATATAATCCAAAATCATGGCGAACCTATTACTCTTCCTGAGCTTGTCTCGGCTCTTCGAATTCCAGAAGCAAAAGCCGGTTGTGTTCACAGTGTCATGCGTTTATTGGCGCACAATAAAATCTTCGCAATTGTCAAGATGGACGACAACAAGGAAGCATATGCTCTTTCTCCAACATCAAAGCTTCTTGTGAAAGGAACTGATCATTGTTTAACTTCAATGGTTAAGATGGTAACAAACCCTACCATGGTTGATAAATATTATCAATTGAGCAAATGGACATCTACGGAGGATCTCACAATCTTTGAGACTACATTATGGGATTTCATGCAACAAAATTTGGCTTATGCGAAGCTATTCAATGATGCTATGGAGAGTGATTCTAATATGATGAGGTTTGCAATGAGTGATTGCAAAACAGTTTTTGAGGGCTTAACTTCTTTGGTTGATGTTGGAGGTGGAACCGGAAACACGGTTAAAATTATTTGTGAGGCGTTTCCTACGTTAAAATGTATAGTGTTCGACCTTCCAAATGTGGTAGAAGGATTAACGGGAAATAACTATCTAAGTTTTGTCGGCGGAGATATGTTTGAATCCATCCCTCAAGCTGATGCAATTTTACTCAAGGTACGTATTAATTCAAAGGGAAATGAATGGTTTATTAATTTCCTTTACCTAATTGCGAATCTATATGTCGGAAAAAACCTGAAGTAAAAGTGTAAAACATAGATAAGAAGGGTACCCGTGAACTTAACTTAGGAAGAAAGGTCACCCCACGTATTCATCTTAAAAAGGTGAATTTCTAGCCGTTAGGCTACTTAAAAAGCAAAAAAAAAAACATAGATAAGAAGGGAAAATAGTTATAAAAAGTTGTATTATTCACAATGAATTAGATTCAATCCAAGATAGTATCATAGTTTTTTTTATTTTGTTTTTTTTGAAGGGAAGATAGTATCATAGTTGAAATGACATTTTTCATCTTACAAATTAATTTTGTAAAAATAAGCTAATGTCAATCCATACATAACAAAGTATTGTTAATCCATACATTATTAATTAGCTTAATTTCCTTTTCATTTTTATAGTGGGTTTTGCATAACTGGAATGATGATGAATGTGTAAAGATACTTAAAAATTGTAAAGAAGCTGCTTCAAGGCAAAACAAAGGAGGAAAAGTGATCATAATAGACATTGTGATAAATGAAAAGGTAGATGAGCATGAAGTAACAGAAGTCAAACTCTGCTTTGATATAACTATGATGGCTAATCACAATTCAAGAGAAAGAGATGAAAAAACATGGAAGAAAATCATAACAGAAGCAGGATTCATGTGTTACAAAATATTTCCTATATTTGGTTTTAGGTCACTTATTGAACTTTCTGTTTAA

Protein sequence

>MS.gene74072.t1

MGSENDCKASELFEAQAHLYNQLFSFMRPASIKWAVELGIPDIIQNHGEPITLPELVSALRIPEAKAGCVHSVMRLLAHNKIFAIVKMDDNKEAYALSPTSKLLVKGTDHCLTSMVKMVTNPTMVDKYYQLSKWTSTEDLTIFETTLWDFMQQNLAYAKLFNDAMESDSNMMRFAMSDCKTVFEGLTSLVDVGGGTGNTVKIICEAFPTLKCIVFDLPNVVEGLTGNNYLSFVGGDMFESIPQADAILLKWVLHNWNDDECVKILKNCKEAASRQNKGGKVIIIDIVINEKVDEHEVTEVKLCFDITMMANHNSRERDEKTWKKIITEAGFMCYKIFPIFGFRSLIELSV