Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74989.t1 | RHN82494.1 | 96.1 | 77 | 3 | 0 | 1 | 77 | 57 | 133 | 5.80E-29 | 136.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74989.t1 | G7ZYS9 | 96.1 | 77 | 3 | 0 | 1 | 77 | 1 | 77 | 4.2e-29 | 136.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene74989 | 0.800838 | 1.19E-48 | -1.69E-46 |
| MS.gene049680 | MS.gene74989 | 0.800157 | 1.64E-48 | -1.69E-46 |
| MS.gene049825 | MS.gene74989 | 0.8074 | 5.15E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene74989 | 0.81035 | 1.20E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene74989 | 0.850403 | 1.74E-60 | -1.69E-46 |
| MS.gene050838 | MS.gene74989 | 0.817849 | 2.66E-52 | -1.69E-46 |
| MS.gene050955 | MS.gene74989 | 0.844176 | 8.89E-59 | -1.69E-46 |
| MS.gene051003 | MS.gene74989 | 0.83771 | 4.43E-57 | -1.69E-46 |
| MS.gene051234 | MS.gene74989 | 0.819101 | 1.39E-52 | -1.69E-46 |
| MS.gene051328 | MS.gene74989 | 0.800919 | 1.15E-48 | -1.69E-46 |
| MS.gene051343 | MS.gene74989 | 0.817943 | 2.54E-52 | -1.69E-46 |
| MS.gene05134 | MS.gene74989 | 0.831727 | 1.42E-55 | -1.69E-46 |
| MS.gene051676 | MS.gene74989 | 0.81349 | 2.49E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene74989 | 0.832436 | 9.46E-56 | -1.69E-46 |
| MS.gene051746 | MS.gene74989 | 0.812652 | 3.81E-51 | -1.69E-46 |
| MS.gene051748 | MS.gene74989 | 0.814667 | 1.37E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene74989 | 0.832773 | 7.81E-56 | -1.69E-46 |
| MS.gene051902 | MS.gene74989 | 0.823513 | 1.33E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene74989 | 0.84241 | 2.63E-58 | -1.69E-46 |
| MS.gene051975 | MS.gene74989 | 0.862906 | 3.64E-64 | -1.69E-46 |
| MS.gene052072 | MS.gene74989 | 0.830269 | 3.23E-55 | -1.69E-46 |
| MS.gene052479 | MS.gene74989 | 0.82173 | 3.46E-53 | -1.69E-46 |
| MS.gene052947 | MS.gene74989 | 0.882477 | 1.01E-70 | -1.69E-46 |
| MS.gene053058 | MS.gene74989 | 0.810685 | 1.02E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene74989 | 0.802145 | 6.45E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene74989 | 0.812115 | 4.99E-51 | -1.69E-46 |
| MS.gene053952 | MS.gene74989 | 0.808671 | 2.76E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene74989 | 0.854105 | 1.54E-61 | -1.69E-46 |
| MS.gene05469 | MS.gene74989 | 0.821201 | 4.58E-53 | -1.69E-46 |
| MS.gene05517 | MS.gene74989 | 0.810968 | 8.85E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene74989 | 0.800309 | 1.53E-48 | -1.69E-46 |
| MS.gene055555 | MS.gene74989 | 0.863059 | 3.26E-64 | -1.69E-46 |
| MS.gene055808 | MS.gene74989 | 0.804441 | 2.16E-49 | -1.69E-46 |
| MS.gene056075 | MS.gene74989 | 0.807633 | 4.59E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene74989 | 0.830314 | 3.15E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene74989 | 0.814626 | 1.40E-51 | -1.69E-46 |
| MS.gene056631 | MS.gene74989 | 0.83377 | 4.41E-56 | -1.69E-46 |
| MS.gene05689 | MS.gene74989 | 0.833668 | 4.67E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene74989 | 0.865037 | 7.89E-65 | -1.69E-46 |
| MS.gene057102 | MS.gene74989 | 0.852446 | 4.59E-61 | -1.69E-46 |
| MS.gene057234 | MS.gene74989 | 0.806323 | 8.70E-50 | -1.69E-46 |
| MS.gene057694 | MS.gene74989 | 0.84204 | 3.30E-58 | -1.69E-46 |
| MS.gene057705 | MS.gene74989 | 0.88233 | 1.14E-70 | -1.69E-46 |
| MS.gene05781 | MS.gene74989 | 0.82069 | 6.00E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene74989 | 0.821248 | 4.47E-53 | -1.69E-46 |
| MS.gene058074 | MS.gene74989 | 0.820963 | 5.19E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene74989 | 0.800124 | 1.67E-48 | -1.69E-46 |
| MS.gene059146 | MS.gene74989 | 0.856658 | 2.77E-62 | -1.69E-46 |
| MS.gene059227 | MS.gene74989 | 0.846314 | 2.35E-59 | -1.69E-46 |
| MS.gene05954 | MS.gene74989 | 0.800484 | 1.41E-48 | -1.69E-46 |
| MS.gene05985 | MS.gene74989 | -0.820726 | 5.89E-53 | -1.69E-46 |
| MS.gene059901 | MS.gene74989 | -0.805098 | 1.57E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene74989 | 0.831864 | 1.31E-55 | -1.69E-46 |
| MS.gene06011 | MS.gene74989 | 0.825112 | 5.60E-54 | -1.69E-46 |
| MS.gene060365 | MS.gene74989 | 0.831569 | 1.55E-55 | -1.69E-46 |
| MS.gene060429 | MS.gene74989 | 0.854982 | 8.56E-62 | -1.69E-46 |
| MS.gene060586 | MS.gene74989 | 0.807441 | 5.05E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene74989 | 0.840745 | 7.23E-58 | -1.69E-46 |
| MS.gene060830 | MS.gene74989 | 0.813092 | 3.05E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene74989 | 0.829564 | 4.80E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74989.t1 | MTR_1g111840 | 96.104 | 77 | 3 | 0 | 1 | 77 | 1 | 77 | 4.87e-50 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74989.t1 | AT5G04750 | 58.730 | 63 | 23 | 3 | 13 | 73 | 25 | 86 | 2.49e-13 | 59.7 |
Find 20 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTTGGTACCTTGTTAT+CGG | 0.335217 | 1.3:+75892045 | None:intergenic |
| ATCTGTAGGTTTCTCCGCCT+TGG | 0.365908 | 1.3:+75892072 | None:intergenic |
| CAAAGAAAACGTCTACATTC+AGG | 0.413338 | 1.3:-75892222 | MS.gene74989:intron |
| GAAATGGGAGAGGGAAAGGT+TGG | 0.423182 | 1.3:-75892122 | MS.gene74989:CDS |
| ACAGAAGCTAGCGGCTGATA+AGG | 0.427012 | 1.3:-75892095 | MS.gene74989:CDS |
| AGGGATCGTGACTCGTTCAA+TGG | 0.456515 | 1.3:-75892318 | MS.gene74989:CDS |
| TTGTTATCGGCATTATCTGT+AGG | 0.488471 | 1.3:+75892058 | None:intergenic |
| TCTCGCTTCTTCAGCGGCAA+AGG | 0.525127 | 1.3:-75892272 | MS.gene74989:CDS |
| TTTGTGTGTAGAAATGGGAG+AGG | 0.537501 | 1.3:-75892132 | MS.gene74989:intron |
| TTGTGTGTAGAAATGGGAGA+GGG | 0.546268 | 1.3:-75892131 | MS.gene74989:intron |
| TGTAGAAATGGGAGAGGGAA+AGG | 0.549281 | 1.3:-75892126 | MS.gene74989:intron |
| TACAGATAATGCCGATAACA+AGG | 0.591322 | 1.3:-75892056 | None:intergenic |
| TCAATGGATTCAACATCTCG+TGG | 0.600776 | 1.3:-75892302 | MS.gene74989:CDS |
| TGAGATCAGCACTGAGTCGA+GGG | 0.604557 | 1.3:-75892337 | MS.gene74989:CDS |
| ATGAGATCAGCACTGAGTCG+AGG | 0.608842 | 1.3:-75892338 | MS.gene74989:CDS |
| GCTTCTTCTCGCTTCTTCAG+CGG | 0.618308 | 1.3:-75892278 | MS.gene74989:CDS |
| GCTAGCGGCTGATAAGGCCA+AGG | 0.640541 | 1.3:-75892089 | MS.gene74989:CDS |
| CAAAGGTAAGATTCTTAGCG+AGG | 0.644514 | 1.3:-75892255 | MS.gene74989:CDS |
| AGCGGCTGATAAGGCCAAGG+CGG | 0.664107 | 1.3:-75892086 | MS.gene74989:CDS |
| GTTGGAGAAACAGAAGCTAG+CGG | 0.726434 | 1.3:-75892104 | MS.gene74989:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAGGATGAAGAAAAA+GGG | + | chr1.3:75892217-75892236 | None:intergenic | 20.0% |
| ! | AAAAAAGGATGAAGAAAAAG+GGG | + | chr1.3:75892216-75892235 | None:intergenic | 25.0% |
| ! | CAAGAAATTGGATCTAAAAA+AGG | + | chr1.3:75892231-75892250 | None:intergenic | 25.0% |
| ! | CTAAAAAAGGATGAAGAAAA+AGG | + | chr1.3:75892218-75892237 | None:intergenic | 25.0% |
| !! | TGATTTTTGTGTGTAGAAAT+GGG | - | chr1.3:75892264-75892283 | MS.gene74989:CDS | 25.0% |
| !! | TTGATTTTTGTGTGTAGAAA+TGG | - | chr1.3:75892263-75892282 | MS.gene74989:CDS | 25.0% |
| AAAAAGGATGAAGAAAAAGG+GGG | + | chr1.3:75892215-75892234 | None:intergenic | 30.0% | |
| CAAAGAAAACGTCTACATTC+AGG | - | chr1.3:75892179-75892198 | MS.gene74989:intron | 35.0% | |
| TTGTTATCGGCATTATCTGT+AGG | + | chr1.3:75892346-75892365 | None:intergenic | 35.0% | |
| ! | CCAATTTCTTGCTTCTGAAA+CGG | - | chr1.3:75892240-75892259 | MS.gene74989:CDS | 35.0% |
| ! | CTGAATGTAGACGTTTTCTT+TGG | + | chr1.3:75892181-75892200 | None:intergenic | 35.0% |
| CAAAGGTAAGATTCTTAGCG+AGG | - | chr1.3:75892146-75892165 | MS.gene74989:intron | 40.0% | |
| CCGTTTCAGAAGCAAGAAAT+TGG | + | chr1.3:75892243-75892262 | None:intergenic | 40.0% | |
| TTGTGTGTAGAAATGGGAGA+GGG | - | chr1.3:75892270-75892289 | MS.gene74989:CDS | 40.0% | |
| TTTGTGTGTAGAAATGGGAG+AGG | - | chr1.3:75892269-75892288 | MS.gene74989:CDS | 40.0% | |
| ! | TCAATGGATTCAACATCTCG+TGG | - | chr1.3:75892099-75892118 | MS.gene74989:CDS | 40.0% |
| GTTGGAGAAACAGAAGCTAG+CGG | - | chr1.3:75892297-75892316 | MS.gene74989:CDS | 45.0% | |
| TGTAGAAATGGGAGAGGGAA+AGG | - | chr1.3:75892275-75892294 | MS.gene74989:CDS | 45.0% | |
| ACAGAAGCTAGCGGCTGATA+AGG | - | chr1.3:75892306-75892325 | MS.gene74989:CDS | 50.0% | |
| AGGGATCGTGACTCGTTCAA+TGG | - | chr1.3:75892083-75892102 | MS.gene74989:CDS | 50.0% | |
| ATCTGTAGGTTTCTCCGCCT+TGG | + | chr1.3:75892332-75892351 | None:intergenic | 50.0% | |
| GAAATGGGAGAGGGAAAGGT+TGG | - | chr1.3:75892279-75892298 | MS.gene74989:CDS | 50.0% | |
| GCTTCTTCTCGCTTCTTCAG+CGG | - | chr1.3:75892123-75892142 | MS.gene74989:CDS | 50.0% | |
| ! | ATGAGATCAGCACTGAGTCG+AGG | - | chr1.3:75892063-75892082 | MS.gene74989:CDS | 50.0% |
| ! | TGAGATCAGCACTGAGTCGA+GGG | - | chr1.3:75892064-75892083 | MS.gene74989:CDS | 50.0% |
| TCTCGCTTCTTCAGCGGCAA+AGG | - | chr1.3:75892129-75892148 | MS.gene74989:intron | 55.0% | |
| AGCGGCTGATAAGGCCAAGG+CGG | - | chr1.3:75892315-75892334 | MS.gene74989:CDS | 60.0% | |
| GCTAGCGGCTGATAAGGCCA+AGG | - | chr1.3:75892312-75892331 | MS.gene74989:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 75892057 | 75892366 | 75892057 | ID=MS.gene74989 |
| chr1.3 | mRNA | 75892057 | 75892366 | 75892057 | ID=MS.gene74989.t1;Parent=MS.gene74989 |
| chr1.3 | exon | 75892223 | 75892366 | 75892223 | ID=MS.gene74989.t1.exon1;Parent=MS.gene74989.t1 |
| chr1.3 | CDS | 75892223 | 75892366 | 75892223 | ID=cds.MS.gene74989.t1;Parent=MS.gene74989.t1 |
| chr1.3 | exon | 75892057 | 75892143 | 75892057 | ID=MS.gene74989.t1.exon2;Parent=MS.gene74989.t1 |
| chr1.3 | CDS | 75892057 | 75892143 | 75892057 | ID=cds.MS.gene74989.t1;Parent=MS.gene74989.t1 |
| Gene Sequence |
| Protein sequence |
