Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75472.t1 | XP_003601959.2 | 85.9 | 199 | 28 | 0 | 1 | 199 | 1 | 199 | 4.10E-95 | 357.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75472.t1 | G7J3S5 | 85.9 | 199 | 28 | 0 | 1 | 199 | 1 | 199 | 3.0e-95 | 357.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene75472 | 0.825892 | 3.66E-54 | -1.69E-46 |
| MS.gene049549 | MS.gene75472 | 0.881396 | 2.48E-70 | -1.69E-46 |
| MS.gene049813 | MS.gene75472 | 0.839333 | 1.69E-57 | -1.69E-46 |
| MS.gene050647 | MS.gene75472 | 0.808701 | 2.72E-50 | -1.69E-46 |
| MS.gene050720 | MS.gene75472 | 0.813521 | 2.45E-51 | -1.69E-46 |
| MS.gene051127 | MS.gene75472 | 0.825481 | 4.58E-54 | -1.69E-46 |
| MS.gene051131 | MS.gene75472 | 0.812456 | 4.20E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene75472 | 0.810745 | 9.89E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene75472 | 0.811164 | 8.03E-51 | -1.69E-46 |
| MS.gene051844 | MS.gene75472 | 0.847842 | 8.95E-60 | -1.69E-46 |
| MS.gene051973 | MS.gene75472 | 0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene75472 | 0.958707 | 1.21E-116 | -1.69E-46 |
| MS.gene052212 | MS.gene75472 | 0.883919 | 2.99E-71 | -1.69E-46 |
| MS.gene05240 | MS.gene75472 | 0.834686 | 2.60E-56 | -1.69E-46 |
| MS.gene05244 | MS.gene75472 | 0.806087 | 9.75E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene75472 | 0.845761 | 3.32E-59 | -1.69E-46 |
| MS.gene052705 | MS.gene75472 | 0.804994 | 1.65E-49 | -1.69E-46 |
| MS.gene053031 | MS.gene75472 | 0.80664 | 7.46E-50 | -1.69E-46 |
| MS.gene053403 | MS.gene75472 | 0.825875 | 3.69E-54 | -1.69E-46 |
| MS.gene053406 | MS.gene75472 | 0.869548 | 2.85E-66 | -1.69E-46 |
| MS.gene053420 | MS.gene75472 | 0.82377 | 1.16E-53 | -1.69E-46 |
| MS.gene053887 | MS.gene75472 | 0.836244 | 1.05E-56 | -1.69E-46 |
| MS.gene054146 | MS.gene75472 | 0.896655 | 2.99E-76 | -1.69E-46 |
| MS.gene054147 | MS.gene75472 | 0.812014 | 5.25E-51 | -1.69E-46 |
| MS.gene054181 | MS.gene75472 | 0.825166 | 5.44E-54 | -1.69E-46 |
| MS.gene054277 | MS.gene75472 | 0.816389 | 5.67E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene75472 | -0.808056 | 3.74E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene75472 | 0.839547 | 1.48E-57 | -1.69E-46 |
| MS.gene05483 | MS.gene75472 | 0.805737 | 1.16E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene75472 | 0.815006 | 1.15E-51 | -1.69E-46 |
| MS.gene055158 | MS.gene75472 | 0.811805 | 5.82E-51 | -1.69E-46 |
| MS.gene055240 | MS.gene75472 | 0.863713 | 2.05E-64 | -1.69E-46 |
| MS.gene05589 | MS.gene75472 | -0.813351 | 2.67E-51 | -1.69E-46 |
| MS.gene055977 | MS.gene75472 | 0.811321 | 7.42E-51 | -1.69E-46 |
| MS.gene056076 | MS.gene75472 | 0.808579 | 2.89E-50 | -1.69E-46 |
| MS.gene056218 | MS.gene75472 | 0.846136 | 2.63E-59 | -1.69E-46 |
| MS.gene056224 | MS.gene75472 | 0.812602 | 3.90E-51 | -1.69E-46 |
| MS.gene056298 | MS.gene75472 | 0.801954 | 7.05E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene75472 | 0.833578 | 4.92E-56 | -1.69E-46 |
| MS.gene056591 | MS.gene75472 | 0.831916 | 1.27E-55 | -1.69E-46 |
| MS.gene056715 | MS.gene75472 | 0.801328 | 9.48E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene75472 | 0.863219 | 2.91E-64 | -1.69E-46 |
| MS.gene056856 | MS.gene75472 | 0.804683 | 1.92E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene75472 | 0.83158 | 1.54E-55 | -1.69E-46 |
| MS.gene056922 | MS.gene75472 | 0.830505 | 2.83E-55 | -1.69E-46 |
| MS.gene056988 | MS.gene75472 | 0.806832 | 6.79E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene75472 | 0.820019 | 8.55E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene75472 | 0.818377 | 2.02E-52 | -1.69E-46 |
| MS.gene057550 | MS.gene75472 | 0.824084 | 9.77E-54 | -1.69E-46 |
| MS.gene057768 | MS.gene75472 | 0.810857 | 9.36E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene75472 | 0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene75472 | 0.801427 | 9.05E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene75472 | 0.802302 | 5.98E-49 | -1.69E-46 |
| MS.gene058381 | MS.gene75472 | 0.814106 | 1.82E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene75472 | 0.830437 | 2.94E-55 | -1.69E-46 |
| MS.gene059328 | MS.gene75472 | 0.826027 | 3.40E-54 | -1.69E-46 |
| MS.gene059402 | MS.gene75472 | 0.801272 | 9.74E-49 | -1.69E-46 |
| MS.gene059953 | MS.gene75472 | 0.841725 | 3.99E-58 | -1.69E-46 |
| MS.gene059955 | MS.gene75472 | 0.878761 | 2.16E-69 | -1.69E-46 |
| MS.gene060087 | MS.gene75472 | 0.839306 | 1.71E-57 | -1.69E-46 |
| MS.gene060358 | MS.gene75472 | 0.820969 | 5.18E-53 | -1.69E-46 |
| MS.gene060593 | MS.gene75472 | 0.831351 | 1.75E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene75472 | -0.804541 | 2.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75472.t1 | MTR_3g087270 | 93.970 | 199 | 12 | 0 | 1 | 199 | 1 | 199 | 9.20e-141 | 390 |
| MS.gene75472.t1 | MTR_3g087280 | 88.945 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 9.71e-133 | 370 |
| MS.gene75472.t1 | MTR_3g087278 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 1.12e-131 | 368 |
| MS.gene75472.t1 | MTR_3g087330 | 84.925 | 199 | 30 | 0 | 1 | 199 | 1 | 199 | 1.26e-127 | 357 |
| MS.gene75472.t1 | MTR_3g087325 | 86.935 | 199 | 26 | 0 | 1 | 199 | 1 | 199 | 5.58e-127 | 356 |
| MS.gene75472.t1 | MTR_3g087275 | 85.930 | 199 | 27 | 1 | 1 | 199 | 1 | 198 | 2.62e-123 | 347 |
| MS.gene75472.t1 | MTR_3g087300 | 66.497 | 197 | 22 | 3 | 1 | 197 | 1 | 153 | 1.77e-86 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75472.t1 | AT5G65030 | 30.964 | 197 | 124 | 3 | 11 | 199 | 10 | 202 | 1.94e-26 | 100 |
| MS.gene75472.t1 | AT5G10210 | 32.124 | 193 | 109 | 7 | 15 | 196 | 13 | 194 | 1.97e-24 | 95.5 |
Find 37 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTGCCCCAAAGAATTCT+TGG | 0.233772 | 3.3:-72767605 | MS.gene75472:CDS |
| CTACCAAATCAGTACCCTTT+TGG | 0.251571 | 3.3:-72767651 | MS.gene75472:CDS |
| ATAAGAGTAGGTTGAATAAT+TGG | 0.258714 | 3.3:-72767336 | MS.gene75472:CDS |
| TGCGCTTGGCGCAGCTTTGG+AGG | 0.349292 | 3.3:-72767245 | MS.gene75472:CDS |
| TTAATCTCCACTTCTACTTC+CGG | 0.353807 | 3.3:+72767397 | None:intergenic |
| AGCTAAGGCAAAGGAAGATA+TGG | 0.356544 | 3.3:-72767552 | MS.gene75472:CDS |
| ATTTGCGCTTGGCGCAGCTT+TGG | 0.402289 | 3.3:-72767248 | MS.gene75472:CDS |
| GCTCCAACACTAGGCTTTGT+TGG | 0.406615 | 3.3:+72767572 | None:intergenic |
| AGTGGCTTCGGTTCATGAAA+TGG | 0.417821 | 3.3:-72767368 | MS.gene75472:CDS |
| GTCCCAAAAGGGTACTGATT+TGG | 0.428896 | 3.3:+72767648 | None:intergenic |
| GATTCAAGAATCACTTTCCA+TGG | 0.437928 | 3.3:+72767499 | None:intergenic |
| GCAAGGCTGTATCTTCCAAC+AGG | 0.447411 | 3.3:-72767712 | MS.gene75472:CDS |
| GATTAAAATAAGAGTGGCTT+CGG | 0.448073 | 3.3:-72767380 | MS.gene75472:CDS |
| TGCACCAAGCGCGATGATCA+TGG | 0.461361 | 3.3:+72767290 | None:intergenic |
| GCTAAGGCAAAGGAAGATAT+GGG | 0.463318 | 3.3:-72767551 | MS.gene75472:CDS |
| ATTAAATGACTCGTCCCAAA+AGG | 0.469911 | 3.3:+72767636 | None:intergenic |
| TTGGAAAAGGTGAGATTCCA+TGG | 0.470388 | 3.3:-72767516 | MS.gene75472:CDS |
| AAAGCCTAGTGTTGGAGCTA+AGG | 0.499139 | 3.3:-72767567 | MS.gene75472:CDS |
| TTTGCCTTAGCTCCAACACT+AGG | 0.507447 | 3.3:+72767563 | None:intergenic |
| CAACTGAAGTTTGCATATGC+AGG | 0.509989 | 3.3:+72767796 | None:intergenic |
| GGGTCACAACTTATTGGAAA+AGG | 0.523568 | 3.3:-72767529 | MS.gene75472:CDS |
| TTAAATGACTCGTCCCAAAA+GGG | 0.547621 | 3.3:+72767637 | None:intergenic |
| AGTGGAGATTAAAATAAGAG+TGG | 0.548596 | 3.3:-72767386 | MS.gene75472:CDS |
| AATGAGTGTGGATGCAAGAA+TGG | 0.549203 | 3.3:-72767313 | MS.gene75472:CDS |
| ATGGCCATGATCATCGCGCT+TGG | 0.550412 | 3.3:-72767294 | MS.gene75472:CDS |
| GATTGTGATATATTTGCGCT+TGG | 0.560902 | 3.3:-72767259 | MS.gene75472:CDS |
| TAACCAACAAAGCCTAGTGT+TGG | 0.564525 | 3.3:-72767575 | MS.gene75472:CDS |
| TTGAATAATTGGAATGAGTG+TGG | 0.579695 | 3.3:-72767325 | MS.gene75472:CDS |
| GATTCTTGAATCACAAAACA+TGG | 0.583125 | 3.3:-72767488 | MS.gene75472:CDS |
| TAGTGTTGGAGCTAAGGCAA+AGG | 0.590191 | 3.3:-72767561 | MS.gene75472:CDS |
| CTCAACACCGGAAGTAGAAG+TGG | 0.592858 | 3.3:-72767404 | MS.gene75472:CDS |
| AGAGGAAATGTTCTCAACAC+CGG | 0.624098 | 3.3:-72767416 | MS.gene75472:CDS |
| TTGAAGATGTATTTAGTGAG+TGG | 0.629374 | 3.3:-72767451 | MS.gene75472:CDS |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.644927 | 3.3:-72767434 | MS.gene75472:CDS |
| AAACGTTGAATTCATCAAGT+CGG | 0.660973 | 3.3:-72767749 | MS.gene75472:CDS |
| CTAAGGCAAAGGAAGATATG+GGG | 0.679009 | 3.3:-72767550 | MS.gene75472:CDS |
| TAAGGCAAAGGAAGATATGG+GGG | 0.682962 | 3.3:-72767549 | MS.gene75472:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACATGGAGTTGAAAAAA+TGG | - | chr3.3:72767577-72767596 | MS.gene75472:CDS | 25.0% |
| ! | TGGAAAAGAAAAATAAGAGT+AGG | - | chr3.3:72767703-72767722 | MS.gene75472:CDS | 25.0% |
| !! | AAGATTTTCCAAGAATTCTT+TGG | + | chr3.3:72767457-72767476 | None:intergenic | 25.0% |
| !! | AGATTTTCCAAGAATTCTTT+GGG | + | chr3.3:72767456-72767475 | None:intergenic | 25.0% |
| !! | ATAAGAGTAGGTTGAATAAT+TGG | - | chr3.3:72767715-72767734 | MS.gene75472:CDS | 25.0% |
| AAACGTTGAATTCATCAAGT+CGG | - | chr3.3:72767302-72767321 | MS.gene75472:CDS | 30.0% | |
| AGTGGAGATTAAAATAAGAG+TGG | - | chr3.3:72767665-72767684 | MS.gene75472:CDS | 30.0% | |
| GATTAAAATAAGAGTGGCTT+CGG | - | chr3.3:72767671-72767690 | MS.gene75472:CDS | 30.0% | |
| GATTCTTGAATCACAAAACA+TGG | - | chr3.3:72767563-72767582 | MS.gene75472:CDS | 30.0% | |
| TTGAAGATGTATTTAGTGAG+TGG | - | chr3.3:72767600-72767619 | MS.gene75472:CDS | 30.0% | |
| TTGAATAATTGGAATGAGTG+TGG | - | chr3.3:72767726-72767745 | MS.gene75472:CDS | 30.0% | |
| ! | GATTTTCCAAGAATTCTTTG+GGG | + | chr3.3:72767455-72767474 | None:intergenic | 30.0% |
| !!! | ATACTTTTGTTGTTTCCTGT+TGG | + | chr3.3:72767357-72767376 | None:intergenic | 30.0% |
| ATTAAATGACTCGTCCCAAA+AGG | + | chr3.3:72767418-72767437 | None:intergenic | 35.0% | |
| GATTCAAGAATCACTTTCCA+TGG | + | chr3.3:72767555-72767574 | None:intergenic | 35.0% | |
| GATTGTGATATATTTGCGCT+TGG | - | chr3.3:72767792-72767811 | MS.gene75472:CDS | 35.0% | |
| TTAAATGACTCGTCCCAAAA+GGG | + | chr3.3:72767417-72767436 | None:intergenic | 35.0% | |
| TTAATCTCCACTTCTACTTC+CGG | + | chr3.3:72767657-72767676 | None:intergenic | 35.0% | |
| ! | TACCAAATCAGTACCCTTTT+GGG | - | chr3.3:72767401-72767420 | MS.gene75472:CDS | 35.0% |
| AATGAGTGTGGATGCAAGAA+TGG | - | chr3.3:72767738-72767757 | MS.gene75472:CDS | 40.0% | |
| AGAGGAAATGTTCTCAACAC+CGG | - | chr3.3:72767635-72767654 | MS.gene75472:CDS | 40.0% | |
| AGCTAAGGCAAAGGAAGATA+TGG | - | chr3.3:72767499-72767518 | MS.gene75472:CDS | 40.0% | |
| ATATGGGGGTCACAACTTAT+TGG | - | chr3.3:72767516-72767535 | MS.gene75472:CDS | 40.0% | |
| CAACTGAAGTTTGCATATGC+AGG | + | chr3.3:72767258-72767277 | None:intergenic | 40.0% | |
| CGGCAAAAAACATGTTTGCA+AGG | - | chr3.3:72767322-72767341 | MS.gene75472:CDS | 40.0% | |
| CTAAGGCAAAGGAAGATATG+GGG | - | chr3.3:72767501-72767520 | MS.gene75472:CDS | 40.0% | |
| GCTAAGGCAAAGGAAGATAT+GGG | - | chr3.3:72767500-72767519 | MS.gene75472:CDS | 40.0% | |
| GGGTCACAACTTATTGGAAA+AGG | - | chr3.3:72767522-72767541 | MS.gene75472:CDS | 40.0% | |
| TAACCAACAAAGCCTAGTGT+TGG | - | chr3.3:72767476-72767495 | MS.gene75472:CDS | 40.0% | |
| TAAGGCAAAGGAAGATATGG+GGG | - | chr3.3:72767502-72767521 | MS.gene75472:CDS | 40.0% | |
| TTCTTGCCCCAAAGAATTCT+TGG | - | chr3.3:72767446-72767465 | MS.gene75472:CDS | 40.0% | |
| TTGGAAAAGGTGAGATTCCA+TGG | - | chr3.3:72767535-72767554 | MS.gene75472:CDS | 40.0% | |
| ! | CTACCAAATCAGTACCCTTT+TGG | - | chr3.3:72767400-72767419 | MS.gene75472:CDS | 40.0% |
| AGTGGCTTCGGTTCATGAAA+TGG | - | chr3.3:72767683-72767702 | MS.gene75472:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | chr3.3:72767617-72767636 | MS.gene75472:CDS | 45.0% | |
| TTTGCCTTAGCTCCAACACT+AGG | + | chr3.3:72767491-72767510 | None:intergenic | 45.0% | |
| ! | AAAGCCTAGTGTTGGAGCTA+AGG | - | chr3.3:72767484-72767503 | MS.gene75472:CDS | 45.0% |
| ! | GCATATGCAGGTGTTTTCGA+TGG | + | chr3.3:72767246-72767265 | None:intergenic | 45.0% |
| ! | TAGTGTTGGAGCTAAGGCAA+AGG | - | chr3.3:72767490-72767509 | MS.gene75472:CDS | 45.0% |
| !! | GTCCCAAAAGGGTACTGATT+TGG | + | chr3.3:72767406-72767425 | None:intergenic | 45.0% |
| CTCAACACCGGAAGTAGAAG+TGG | - | chr3.3:72767647-72767666 | MS.gene75472:CDS | 50.0% | |
| GCAAGGCTGTATCTTCCAAC+AGG | - | chr3.3:72767339-72767358 | MS.gene75472:CDS | 50.0% | |
| ! | GCTCCAACACTAGGCTTTGT+TGG | + | chr3.3:72767482-72767501 | None:intergenic | 50.0% |
| ATGGCCATGATCATCGCGCT+TGG | - | chr3.3:72767757-72767776 | MS.gene75472:CDS | 55.0% | |
| ATTTGCGCTTGGCGCAGCTT+TGG | - | chr3.3:72767803-72767822 | MS.gene75472:CDS | 55.0% | |
| TGCACCAAGCGCGATGATCA+TGG | + | chr3.3:72767764-72767783 | None:intergenic | 55.0% | |
| ! | TGCGCTTGGCGCAGCTTTGG+AGG | - | chr3.3:72767806-72767825 | MS.gene75472:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 72767237 | 72767836 | 72767237 | ID=MS.gene75472 |
| chr3.3 | mRNA | 72767237 | 72767836 | 72767237 | ID=MS.gene75472.t1;Parent=MS.gene75472 |
| chr3.3 | exon | 72767237 | 72767836 | 72767237 | ID=MS.gene75472.t1.exon1;Parent=MS.gene75472.t1 |
| chr3.3 | CDS | 72767237 | 72767836 | 72767237 | ID=cds.MS.gene75472.t1;Parent=MS.gene75472.t1 |
| Gene Sequence |
| Protein sequence |
