Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82144.t1 | XP_003612553.1 | 98.1 | 470 | 9 | 0 | 6 | 475 | 104 | 573 | 3.10E-258 | 900.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82144.t1 | F4I9E1 | 55.5 | 465 | 173 | 5 | 6 | 469 | 140 | 571 | 3.2e-134 | 479.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82144.t1 | G7K5G1 | 98.1 | 470 | 9 | 0 | 6 | 475 | 104 | 573 | 2.3e-258 | 900.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene82144 | 0.811531 | 6.68E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene82144 | 0.804039 | 2.62E-49 | -1.69E-46 |
| MS.gene049232 | MS.gene82144 | 0.815006 | 1.15E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene82144 | 0.80643 | 8.26E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene82144 | 0.823415 | 1.40E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene82144 | 0.828638 | 8.04E-55 | -1.69E-46 |
| MS.gene049865 | MS.gene82144 | 0.805251 | 1.46E-49 | -1.69E-46 |
| MS.gene05010 | MS.gene82144 | 0.803444 | 3.48E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene82144 | 0.84145 | 4.72E-58 | -1.69E-46 |
| MS.gene050323 | MS.gene82144 | 0.852445 | 4.59E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene82144 | 0.82764 | 1.40E-54 | -1.69E-46 |
| MS.gene050593 | MS.gene82144 | 0.829534 | 4.88E-55 | -1.69E-46 |
| MS.gene050697 | MS.gene82144 | 0.812692 | 3.73E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene82144 | 0.803192 | 3.92E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene82144 | 0.837308 | 5.61E-57 | -1.69E-46 |
| MS.gene05081 | MS.gene82144 | 0.808815 | 2.57E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene82144 | 0.833598 | 4.86E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene82144 | 0.83338 | 5.51E-56 | -1.69E-46 |
| MS.gene051838 | MS.gene82144 | 0.826167 | 3.15E-54 | -1.69E-46 |
| MS.gene051858 | MS.gene82144 | 0.810286 | 1.24E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene82144 | 0.803458 | 3.45E-49 | -1.69E-46 |
| MS.gene052346 | MS.gene82144 | 0.817408 | 3.35E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene82144 | 0.806949 | 6.42E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene82144 | 0.828609 | 8.18E-55 | -1.69E-46 |
| MS.gene052504 | MS.gene82144 | 0.810331 | 1.22E-50 | -1.69E-46 |
| MS.gene052506 | MS.gene82144 | 0.839595 | 1.44E-57 | -1.69E-46 |
| MS.gene052820 | MS.gene82144 | 0.806848 | 6.74E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene82144 | 0.838273 | 3.17E-57 | -1.69E-46 |
| MS.gene052823 | MS.gene82144 | 0.826434 | 2.72E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene82144 | 0.824159 | 9.39E-54 | -1.69E-46 |
| MS.gene052966 | MS.gene82144 | 0.843196 | 1.63E-58 | -1.69E-46 |
| MS.gene053203 | MS.gene82144 | 0.818957 | 1.49E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene82144 | 0.800961 | 1.13E-48 | -1.69E-46 |
| MS.gene053449 | MS.gene82144 | 0.82423 | 9.03E-54 | -1.69E-46 |
| MS.gene053479 | MS.gene82144 | 0.833586 | 4.90E-56 | -1.69E-46 |
| MS.gene053480 | MS.gene82144 | 0.819603 | 1.06E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene82144 | 0.828055 | 1.11E-54 | -1.69E-46 |
| MS.gene053633 | MS.gene82144 | 0.806631 | 7.49E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene82144 | 0.876764 | 1.08E-68 | -1.69E-46 |
| MS.gene054595 | MS.gene82144 | 0.811067 | 8.43E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene82144 | 0.812429 | 4.26E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene82144 | 0.823678 | 1.22E-53 | -1.69E-46 |
| MS.gene055224 | MS.gene82144 | 0.826202 | 3.09E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene82144 | 0.801653 | 8.13E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene82144 | 0.824121 | 9.58E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene82144 | 0.819447 | 1.16E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene82144 | 0.822021 | 2.96E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene82144 | 0.81829 | 2.12E-52 | -1.69E-46 |
| MS.gene055941 | MS.gene82144 | 0.834513 | 2.87E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene82144 | 0.840197 | 1.01E-57 | -1.69E-46 |
| MS.gene056510 | MS.gene82144 | 0.814324 | 1.63E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene82144 | 0.800918 | 1.15E-48 | -1.69E-46 |
| MS.gene056878 | MS.gene82144 | 0.810474 | 1.13E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene82144 | 0.830649 | 2.61E-55 | -1.69E-46 |
| MS.gene057086 | MS.gene82144 | 0.834235 | 3.37E-56 | -1.69E-46 |
| MS.gene057856 | MS.gene82144 | 0.846603 | 1.96E-59 | -1.69E-46 |
| MS.gene057857 | MS.gene82144 | 0.808584 | 2.88E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene82144 | 0.801335 | 9.45E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene82144 | 0.818113 | 2.32E-52 | -1.69E-46 |
| MS.gene058338 | MS.gene82144 | 0.802859 | 4.59E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene82144 | 0.852091 | 5.79E-61 | -1.69E-46 |
| MS.gene058419 | MS.gene82144 | 0.812424 | 4.27E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene82144 | 0.812497 | 4.12E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene82144 | 0.834111 | 3.62E-56 | -1.69E-46 |
| MS.gene05913 | MS.gene82144 | 0.801228 | 9.94E-49 | -1.69E-46 |
| MS.gene059305 | MS.gene82144 | 0.815528 | 8.83E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene82144 | 0.804742 | 1.87E-49 | -1.69E-46 |
| MS.gene059801 | MS.gene82144 | 0.805227 | 1.48E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene82144 | 0.802046 | 6.76E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene82144 | 0.808091 | 3.67E-50 | -1.69E-46 |
| MS.gene060778 | MS.gene82144 | 0.823129 | 1.64E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene82144 | 0.823443 | 1.38E-53 | -1.69E-46 |
| MS.gene060835 | MS.gene82144 | 0.834491 | 2.91E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene82144 | 0.814074 | 1.85E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene82144 | 0.829191 | 5.91E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82144.t1 | MTR_5g026350 | 98.085 | 470 | 9 | 0 | 6 | 475 | 104 | 573 | 0.0 | 938 |
| MS.gene82144.t1 | MTR_8g071920 | 71.767 | 464 | 125 | 4 | 14 | 474 | 153 | 613 | 0.0 | 640 |
| MS.gene82144.t1 | MTR_7g083660 | 57.511 | 466 | 182 | 5 | 6 | 467 | 104 | 557 | 5.05e-169 | 488 |
| MS.gene82144.t1 | MTR_3g466040 | 33.913 | 460 | 251 | 12 | 5 | 461 | 104 | 513 | 3.35e-65 | 219 |
| MS.gene82144.t1 | MTR_3g466110 | 33.641 | 434 | 224 | 13 | 5 | 430 | 104 | 481 | 9.18e-59 | 201 |
| MS.gene82144.t1 | MTR_4g073690 | 29.114 | 474 | 273 | 12 | 2 | 461 | 110 | 534 | 7.10e-48 | 173 |
| MS.gene82144.t1 | MTR_5g022380 | 30.277 | 469 | 274 | 14 | 8 | 471 | 4 | 424 | 4.91e-41 | 152 |
| MS.gene82144.t1 | MTR_5g022380 | 30.105 | 475 | 279 | 14 | 2 | 471 | 108 | 534 | 2.40e-40 | 152 |
| MS.gene82144.t1 | MTR_4g116210 | 26.552 | 467 | 294 | 14 | 16 | 461 | 121 | 559 | 4.86e-29 | 120 |
| MS.gene82144.t1 | MTR_2g450710 | 25.105 | 478 | 302 | 14 | 6 | 461 | 110 | 553 | 5.89e-26 | 111 |
| MS.gene82144.t1 | MTR_4g073690 | 27.358 | 318 | 178 | 8 | 2 | 307 | 110 | 386 | 7.46e-25 | 106 |
| MS.gene82144.t1 | MTR_5g090570 | 30.357 | 224 | 143 | 5 | 249 | 469 | 319 | 532 | 6.23e-22 | 99.0 |
| MS.gene82144.t1 | MTR_1g111530 | 24.784 | 464 | 303 | 13 | 21 | 471 | 135 | 565 | 3.93e-19 | 90.9 |
| MS.gene82144.t1 | MTR_1g111510 | 24.167 | 480 | 321 | 13 | 5 | 471 | 120 | 569 | 4.33e-19 | 90.5 |
| MS.gene82144.t1 | MTR_7g029015 | 23.326 | 463 | 322 | 12 | 19 | 460 | 126 | 576 | 1.66e-18 | 89.0 |
| MS.gene82144.t1 | MTR_1g111550 | 25.383 | 457 | 300 | 12 | 21 | 467 | 138 | 563 | 5.72e-18 | 87.0 |
| MS.gene82144.t1 | MTR_1g111540 | 24.066 | 482 | 319 | 14 | 5 | 471 | 120 | 569 | 1.35e-17 | 85.9 |
| MS.gene82144.t1 | MTR_3g099150 | 23.218 | 491 | 289 | 17 | 8 | 461 | 115 | 554 | 7.96e-17 | 83.6 |
| MS.gene82144.t1 | MTR_1g050550 | 23.554 | 484 | 320 | 16 | 9 | 471 | 122 | 576 | 4.42e-16 | 81.3 |
| MS.gene82144.t1 | MTR_1g059080 | 22.981 | 483 | 323 | 16 | 5 | 471 | 118 | 567 | 4.59e-16 | 81.3 |
| MS.gene82144.t1 | MTR_7g102880 | 28.992 | 238 | 155 | 9 | 240 | 467 | 344 | 577 | 1.86e-14 | 76.3 |
| MS.gene82144.t1 | MTR_2g100400 | 21.459 | 466 | 308 | 12 | 8 | 469 | 126 | 537 | 4.75e-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82144.t1 | AT5G45275 | 63.753 | 469 | 159 | 3 | 6 | 470 | 104 | 565 | 0.0 | 572 |
| MS.gene82144.t1 | AT4G19450 | 62.368 | 473 | 163 | 4 | 6 | 471 | 104 | 568 | 0.0 | 561 |
| MS.gene82144.t1 | AT1G31470 | 55.054 | 465 | 175 | 6 | 6 | 469 | 140 | 571 | 1.05e-159 | 465 |
| MS.gene82144.t1 | AT3G01630 | 48.941 | 472 | 192 | 12 | 6 | 468 | 120 | 551 | 8.25e-142 | 418 |
| MS.gene82144.t1 | AT3G01630 | 49.045 | 471 | 193 | 11 | 6 | 468 | 138 | 569 | 1.92e-141 | 418 |
| MS.gene82144.t1 | AT4G19450 | 60.317 | 315 | 113 | 3 | 6 | 313 | 104 | 413 | 2.74e-125 | 371 |
| MS.gene82144.t1 | AT2G30300 | 32.080 | 452 | 248 | 11 | 17 | 461 | 98 | 497 | 5.74e-57 | 197 |
| MS.gene82144.t1 | AT4G34950 | 29.437 | 462 | 301 | 12 | 2 | 460 | 111 | 550 | 2.43e-48 | 175 |
| MS.gene82144.t1 | AT2G16660 | 28.879 | 464 | 282 | 12 | 2 | 460 | 109 | 529 | 8.53e-43 | 159 |
| MS.gene82144.t1 | AT5G50520 | 26.477 | 457 | 284 | 14 | 19 | 469 | 128 | 538 | 2.71e-25 | 109 |
| MS.gene82144.t1 | AT5G50630 | 26.477 | 457 | 284 | 14 | 19 | 469 | 128 | 538 | 2.71e-25 | 109 |
| MS.gene82144.t1 | AT2G28120 | 24.236 | 458 | 312 | 10 | 21 | 471 | 130 | 559 | 7.30e-25 | 108 |
| MS.gene82144.t1 | AT1G80530 | 25.758 | 462 | 283 | 15 | 19 | 461 | 124 | 544 | 1.11e-24 | 107 |
| MS.gene82144.t1 | AT2G39210 | 24.845 | 483 | 310 | 15 | 5 | 467 | 115 | 564 | 4.75e-24 | 105 |
| MS.gene82144.t1 | AT2G16660 | 26.899 | 316 | 194 | 9 | 2 | 314 | 109 | 390 | 3.47e-20 | 92.8 |
| MS.gene82144.t1 | AT5G14120 | 25.268 | 467 | 305 | 13 | 19 | 462 | 127 | 572 | 3.53e-20 | 94.0 |
| MS.gene82144.t1 | AT3G01930 | 25.107 | 466 | 308 | 12 | 19 | 460 | 14 | 462 | 9.23e-19 | 89.0 |
| MS.gene82144.t1 | AT3G01930 | 25.107 | 466 | 308 | 12 | 19 | 460 | 127 | 575 | 2.26e-18 | 88.6 |
| MS.gene82144.t1 | AT1G74780 | 28.216 | 241 | 158 | 7 | 232 | 468 | 301 | 530 | 2.00e-16 | 82.0 |
| MS.gene82144.t1 | AT2G34355 | 25.847 | 236 | 163 | 5 | 234 | 467 | 145 | 370 | 5.41e-14 | 73.9 |
| MS.gene82144.t1 | AT1G18940 | 27.459 | 244 | 152 | 10 | 235 | 467 | 296 | 525 | 8.98e-14 | 73.9 |
| MS.gene82144.t1 | AT2G34355 | 25.847 | 236 | 163 | 5 | 234 | 467 | 295 | 520 | 1.76e-13 | 73.2 |
| MS.gene82144.t1 | AT2G34350 | 28.378 | 222 | 139 | 7 | 253 | 468 | 316 | 523 | 9.09e-13 | 70.9 |
| MS.gene82144.t1 | AT2G34350 | 28.378 | 222 | 139 | 7 | 253 | 468 | 316 | 523 | 9.09e-13 | 70.9 |
Find 91 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTCATCGTTCTCCTTCTT+TGG | 0.177256 | 5.2:+17009757 | MS.gene82144:CDS |
| CCCAATTGCATTCATCTTGC+TGG | 0.229813 | 5.2:+17009994 | MS.gene82144:CDS |
| ATCCCTTCTCTATGGTTTCA+TGG | 0.233016 | 5.2:+17010177 | MS.gene82144:CDS |
| GAAGAGGGACAAGGGCATTT+AGG | 0.272558 | 5.2:-17009087 | None:intergenic |
| TGGGACGGCAATGCTATTTC+TGG | 0.278696 | 5.2:+17010278 | MS.gene82144:CDS |
| CCAATTGGATCCCTTCTCTA+TGG | 0.305725 | 5.2:+17010169 | MS.gene82144:CDS |
| ATTATTATGTTTCCATTTCC+AGG | 0.322761 | 5.2:-17010235 | None:intergenic |
| AATGCTAAGGATCTGTTAAC+TGG | 0.327320 | 5.2:-17008971 | None:intergenic |
| CTTGTTTACAGCAATAATCT+TGG | 0.332047 | 5.2:+17009679 | MS.gene82144:CDS |
| GCCGTTACATCTGAGCTCTT+TGG | 0.350791 | 5.2:+17010100 | MS.gene82144:CDS |
| AAAATAATCATAAGCATGTT+TGG | 0.351008 | 5.2:-17010368 | None:intergenic |
| GGACAGATAGCTCAATCTTT+AGG | 0.355909 | 5.2:+17009700 | MS.gene82144:CDS |
| CTCATAACAAATATTCCAAT+TGG | 0.383465 | 5.2:+17010154 | MS.gene82144:CDS |
| CTGCTTGGTGAAAACGGATA+TGG | 0.383934 | 5.2:+17009499 | MS.gene82144:CDS |
| GCCAGCAAGATGAATGCAAT+TGG | 0.384601 | 5.2:-17009995 | None:intergenic |
| GCTATCTCAGCACCTGGAAA+TGG | 0.394685 | 5.2:+17010223 | MS.gene82144:CDS |
| TACTTCTGCGGAGGTACAAT+TGG | 0.409964 | 5.2:+17009655 | MS.gene82144:CDS |
| CAGCCGCGGTTCTTGTGAAA+AGG | 0.416501 | 5.2:+17009605 | MS.gene82144:CDS |
| AAGTTCTACTTTGCAAGGAC+AGG | 0.418566 | 5.2:+17009944 | MS.gene82144:CDS |
| GCTATTTCTGGACATTTGTA+TGG | 0.429375 | 5.2:+17010290 | MS.gene82144:CDS |
| GCAGGATCAATTGGAAATGC+TGG | 0.431793 | 5.2:+17009570 | MS.gene82144:CDS |
| CCAGCAAGATGAATGCAATT+GGG | 0.438820 | 5.2:-17009994 | None:intergenic |
| GCACTCACTGTGAGTTTCAA+TGG | 0.445754 | 5.2:+17008995 | MS.gene82144:CDS |
| GTGTTATATATGCTAGAGAT+TGG | 0.448496 | 5.2:+17009341 | MS.gene82144:CDS |
| TTTCTGGACATTTGTATGGT+GGG | 0.453355 | 5.2:+17010294 | MS.gene82144:CDS |
| AATGTAGGAACTGCACTAAT+CGG | 0.461447 | 5.2:+17010043 | MS.gene82144:CDS |
| CAAGGTCTGCTTGGTGAAAA+CGG | 0.464458 | 5.2:+17009493 | MS.gene82144:CDS |
| TGGGGATGTATATCTGTCAT+AGG | 0.465322 | 5.2:+17010313 | MS.gene82144:CDS |
| ACTTACTTTCGAATGTAGTC+AGG | 0.465887 | 5.2:-17009799 | None:intergenic |
| CAGTCGGTTGCGCTGAAATA+CGG | 0.476009 | 5.2:-17010399 | None:intergenic |
| AGATAGAGTTGATCTGATGA+AGG | 0.476307 | 5.2:-17009061 | None:intergenic |
| ACGTCGAGGCTTTACTTTGG+TGG | 0.478119 | 5.2:+17009280 | MS.gene82144:CDS |
| AAAGGCGGTTGGCGAAGAAT+CGG | 0.485100 | 5.2:-17009133 | None:intergenic |
| TGAAAGCAGGCGTCCAAAGA+AGG | 0.489358 | 5.2:-17009770 | None:intergenic |
| GGAGAATGAGTGTCTGATAA+AGG | 0.489819 | 5.2:-17009151 | None:intergenic |
| CCATAGAGAAGGGATCCAAT+TGG | 0.490493 | 5.2:-17010169 | None:intergenic |
| CAAAGCAGCGCTCGGAGCAA+TGG | 0.493468 | 5.2:+17009463 | MS.gene82144:CDS |
| TTCTGGACATTTGTATGGTG+GGG | 0.499270 | 5.2:+17010295 | MS.gene82144:CDS |
| CGCGGTTCTTGTGAAAAGGT+TGG | 0.500230 | 5.2:+17009609 | MS.gene82144:CDS |
| GTCTGATTCATTAGTGTGCA+TGG | 0.507089 | 5.2:+17010258 | MS.gene82144:CDS |
| GCACTAATCGGTCTGAGCTC+AGG | 0.513327 | 5.2:+17010055 | MS.gene82144:CDS |
| TGAGTGGTTGCAGTATCTGT+TGG | 0.528360 | 5.2:+17008909 | MS.gene82144:CDS |
| GAGCGCTGCTTTGACGCGTA+AGG | 0.529395 | 5.2:-17009453 | None:intergenic |
| GCAGGAAGTTCTACTTTGCA+AGG | 0.530474 | 5.2:+17009939 | MS.gene82144:intron |
| CACAGTGAGTGCCAATGCTA+AGG | 0.532501 | 5.2:-17008984 | None:intergenic |
| GCAACGTCGAGGCTTTACTT+TGG | 0.532929 | 5.2:+17009277 | MS.gene82144:CDS |
| TATTATATTGCATACTTCTG+CGG | 0.537599 | 5.2:+17009643 | MS.gene82144:CDS |
| GATAGAGTTGATCTGATGAA+GGG | 0.542870 | 5.2:-17009060 | None:intergenic |
| GGAGATGCTCAAGGTCTGCT+TGG | 0.546915 | 5.2:+17009484 | MS.gene82144:CDS |
| GGCTTCATCTGAAAGTGCTA+TGG | 0.552373 | 5.2:+17010015 | MS.gene82144:CDS |
| ATTTCTGGACATTTGTATGG+TGG | 0.553978 | 5.2:+17010293 | MS.gene82144:CDS |
| TTTCATATTTGCAGCTGCAG+TGG | 0.554888 | 5.2:+17010078 | MS.gene82144:CDS |
| TGATTGACACTCAAACTGTC+GGG | 0.557476 | 5.2:-17010124 | None:intergenic |
| CGAAGTGAGAAGAGGGACAA+GGG | 0.560371 | 5.2:-17009095 | None:intergenic |
| TTACGCGTCAAAGCAGCGCT+CGG | 0.560674 | 5.2:+17009455 | MS.gene82144:CDS |
| CAGGATCAATTGGAAATGCT+GGG | 0.562484 | 5.2:+17009571 | MS.gene82144:CDS |
| TCCAAAGAGCTCAGATGTAA+CGG | 0.563002 | 5.2:-17010101 | None:intergenic |
| AAGATGAATGCAATTGGGGT+TGG | 0.563489 | 5.2:-17009989 | None:intergenic |
| AGTGCTATGGCACTTAATGT+AGG | 0.563642 | 5.2:+17010028 | MS.gene82144:CDS |
| CTGCGAGCGAAGTGAGAAGA+GGG | 0.581355 | 5.2:-17009102 | None:intergenic |
| GCGAAGTGAGAAGAGGGACA+AGG | 0.583886 | 5.2:-17009096 | None:intergenic |
| ATAGCTCAATCTTTAGGCCA+CGG | 0.585336 | 5.2:+17009706 | MS.gene82144:CDS |
| CAGCCATGAAACCATAGAGA+AGG | 0.585536 | 5.2:-17010180 | None:intergenic |
| CAGCAAGATGAATGCAATTG+GGG | 0.585987 | 5.2:-17009993 | None:intergenic |
| AGTTCTACTTTGCAAGGACA+GGG | 0.588322 | 5.2:+17009945 | MS.gene82144:CDS |
| ACATGCCGCTCGTCAGAACT+CGG | 0.589916 | 5.2:+17009174 | MS.gene82144:CDS |
| AGTTAACAGATCCTTAGCAT+TGG | 0.591254 | 5.2:+17008973 | MS.gene82144:CDS |
| AGTCAGGCATAGCTGAAAGC+AGG | 0.592721 | 5.2:-17009783 | None:intergenic |
| TGGGGTTGGTAAAAGTGCAA+TGG | 0.599533 | 5.2:-17009975 | None:intergenic |
| GCTAATGCTATCTCAGCACC+TGG | 0.600408 | 5.2:+17010217 | MS.gene82144:CDS |
| AAGTTGATGATGCAAAGATA+AGG | 0.600414 | 5.2:-17009246 | None:intergenic |
| CGGAGCAATGGAGATGCTCA+AGG | 0.604999 | 5.2:+17009475 | MS.gene82144:CDS |
| GAGTTCTGACGAGCGGCATG+TGG | 0.606419 | 5.2:-17009172 | None:intergenic |
| ATTCATTAGTGTGCATGGGA+CGG | 0.608695 | 5.2:+17010263 | MS.gene82144:CDS |
| GTGATTGACACTCAAACTGT+CGG | 0.614996 | 5.2:-17010125 | None:intergenic |
| ATAACACCAGGAATGCATAA+CGG | 0.617967 | 5.2:-17009325 | None:intergenic |
| ATGCTAAGGATCTGTTAACT+GGG | 0.628103 | 5.2:-17008970 | None:intergenic |
| AGCCATGAAACCATAGAGAA+GGG | 0.633768 | 5.2:-17010179 | None:intergenic |
| TATATTGCATACTTCTGCGG+AGG | 0.634986 | 5.2:+17009646 | MS.gene82144:CDS |
| GCTGCGAGCGAAGTGAGAAG+AGG | 0.636097 | 5.2:-17009103 | None:intergenic |
| GAGTGTCTGATAAAGGCGGT+TGG | 0.638280 | 5.2:-17009144 | None:intergenic |
| TCTCTAGCATATATAACACC+AGG | 0.642863 | 5.2:-17009337 | None:intergenic |
| GAGTCGACGTTCTATAACCG+TGG | 0.643293 | 5.2:-17009723 | None:intergenic |
| GTCAGAAGTTAAGACACAGT+CGG | 0.643515 | 5.2:-17010415 | None:intergenic |
| TCTGATTCATTAGTGTGCAT+GGG | 0.646463 | 5.2:+17010259 | MS.gene82144:CDS |
| TCTACTTTGCAAGGACAGGG+TGG | 0.676179 | 5.2:+17009948 | MS.gene82144:CDS |
| CTTCTGATGAAGCAACGTCG+AGG | 0.688233 | 5.2:+17009266 | MS.gene82144:CDS |
| GGGAGAAGAACATTCAGCCG+CGG | 0.691345 | 5.2:+17009591 | MS.gene82144:CDS |
| TATAACCGAGTTCTGACGAG+CGG | 0.712307 | 5.2:-17009179 | None:intergenic |
| GAAGGCTCGGATGAACAGCA+CGG | 0.721953 | 5.2:-17009369 | None:intergenic |
| GAATGAGTGTCTGATAAAGG+CGG | 0.762249 | 5.2:-17009148 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAATCATAAGCATGTT+TGG | - | chr5.2:17010371-17010390 | None:intergenic | 20.0% |
| ! | ATTATTATGTTTCCATTTCC+AGG | - | chr5.2:17010238-17010257 | None:intergenic | 25.0% |
| ! | CTCATAACAAATATTCCAAT+TGG | + | chr5.2:17010154-17010173 | MS.gene82144:CDS | 25.0% |
| ! | CTGATATAAACGAAAAAAAC+TGG | + | chr5.2:17009840-17009859 | MS.gene82144:intron | 25.0% |
| ! | TATTATATTGCATACTTCTG+CGG | + | chr5.2:17009643-17009662 | MS.gene82144:CDS | 25.0% |
| !! | GAGACTAAGAGTAAATTTTT+CGG | + | chr5.2:17009911-17009930 | MS.gene82144:intron | 25.0% |
| !! | TCTAACATTGAACATTTTAG+CGG | + | chr5.2:17009204-17009223 | MS.gene82144:CDS | 25.0% |
| !!! | AGCTTTGAATTTTATGTTTC+AGG | + | chr5.2:17008868-17008887 | MS.gene82144:intron | 25.0% |
| !!! | TGAATTGTGACAAGTTTTTT+GGG | + | chr5.2:17009548-17009567 | MS.gene82144:CDS | 25.0% |
| CTTGTTTACAGCAATAATCT+TGG | + | chr5.2:17009679-17009698 | MS.gene82144:CDS | 30.0% | |
| GTGTTATATATGCTAGAGAT+TGG | + | chr5.2:17009341-17009360 | MS.gene82144:CDS | 30.0% | |
| ! | TTGTGAAAAGGTTGGATTTT+TGG | + | chr5.2:17009617-17009636 | MS.gene82144:CDS | 30.0% |
| !! | AACATTTTAGCGGTTTTTAC+AGG | + | chr5.2:17009214-17009233 | MS.gene82144:CDS | 30.0% |
| !! | AAGTTGATGATGCAAAGATA+AGG | - | chr5.2:17009249-17009268 | None:intergenic | 30.0% |
| !! | GTAAATTTTTCGGTATTTGC+AGG | + | chr5.2:17009921-17009940 | MS.gene82144:intron | 30.0% |
| !!! | GTGAATTGTGACAAGTTTTT+TGG | + | chr5.2:17009547-17009566 | MS.gene82144:CDS | 30.0% |
| AATGCTAAGGATCTGTTAAC+TGG | - | chr5.2:17008974-17008993 | None:intergenic | 35.0% | |
| AATGTAGGAACTGCACTAAT+CGG | + | chr5.2:17010043-17010062 | MS.gene82144:CDS | 35.0% | |
| ACTTACTTTCGAATGTAGTC+AGG | - | chr5.2:17009802-17009821 | None:intergenic | 35.0% | |
| AGTTAACAGATCCTTAGCAT+TGG | + | chr5.2:17008973-17008992 | MS.gene82144:CDS | 35.0% | |
| ATAACACCAGGAATGCATAA+CGG | - | chr5.2:17009328-17009347 | None:intergenic | 35.0% | |
| ATGCTAAGGATCTGTTAACT+GGG | - | chr5.2:17008973-17008992 | None:intergenic | 35.0% | |
| TATTCATCGTTCTCCTTCTT+TGG | + | chr5.2:17009757-17009776 | MS.gene82144:CDS | 35.0% | |
| TCTCTAGCATATATAACACC+AGG | - | chr5.2:17009340-17009359 | None:intergenic | 35.0% | |
| TCTGATTCATTAGTGTGCAT+GGG | + | chr5.2:17010259-17010278 | MS.gene82144:CDS | 35.0% | |
| TTTCTGGACATTTGTATGGT+GGG | + | chr5.2:17010294-17010313 | MS.gene82144:CDS | 35.0% | |
| ! | AGATAGAGTTGATCTGATGA+AGG | - | chr5.2:17009064-17009083 | None:intergenic | 35.0% |
| ! | ATTTCTGGACATTTGTATGG+TGG | + | chr5.2:17010293-17010312 | MS.gene82144:CDS | 35.0% |
| ! | GATAGAGTTGATCTGATGAA+GGG | - | chr5.2:17009063-17009082 | None:intergenic | 35.0% |
| !! | GCTATTTCTGGACATTTGTA+TGG | + | chr5.2:17010290-17010309 | MS.gene82144:CDS | 35.0% |
| !!! | TTTTTTCTGTGTCTGTTGAG+TGG | + | chr5.2:17008893-17008912 | MS.gene82144:CDS | 35.0% |
| AAGATGAATGCAATTGGGGT+TGG | - | chr5.2:17009992-17010011 | None:intergenic | 40.0% | |
| AAGTTCTACTTTGCAAGGAC+AGG | + | chr5.2:17009944-17009963 | MS.gene82144:CDS | 40.0% | |
| AGCCATGAAACCATAGAGAA+GGG | - | chr5.2:17010182-17010201 | None:intergenic | 40.0% | |
| AGTGCTATGGCACTTAATGT+AGG | + | chr5.2:17010028-17010047 | MS.gene82144:CDS | 40.0% | |
| AGTTCTACTTTGCAAGGACA+GGG | + | chr5.2:17009945-17009964 | MS.gene82144:CDS | 40.0% | |
| ATAGCTCAATCTTTAGGCCA+CGG | + | chr5.2:17009706-17009725 | MS.gene82144:CDS | 40.0% | |
| ATCCCTTCTCTATGGTTTCA+TGG | + | chr5.2:17010177-17010196 | MS.gene82144:CDS | 40.0% | |
| ATTCATTAGTGTGCATGGGA+CGG | + | chr5.2:17010263-17010282 | MS.gene82144:CDS | 40.0% | |
| CAGCAAGATGAATGCAATTG+GGG | - | chr5.2:17009996-17010015 | None:intergenic | 40.0% | |
| CAGGATCAATTGGAAATGCT+GGG | + | chr5.2:17009571-17009590 | MS.gene82144:CDS | 40.0% | |
| CCAGCAAGATGAATGCAATT+GGG | - | chr5.2:17009997-17010016 | None:intergenic | 40.0% | |
| GAATGAGTGTCTGATAAAGG+CGG | - | chr5.2:17009151-17009170 | None:intergenic | 40.0% | |
| GGACAGATAGCTCAATCTTT+AGG | + | chr5.2:17009700-17009719 | MS.gene82144:CDS | 40.0% | |
| GGAGAATGAGTGTCTGATAA+AGG | - | chr5.2:17009154-17009173 | None:intergenic | 40.0% | |
| GTCTGATTCATTAGTGTGCA+TGG | + | chr5.2:17010258-17010277 | MS.gene82144:CDS | 40.0% | |
| GTGATTGACACTCAAACTGT+CGG | - | chr5.2:17010128-17010147 | None:intergenic | 40.0% | |
| TATATTGCATACTTCTGCGG+AGG | + | chr5.2:17009646-17009665 | MS.gene82144:CDS | 40.0% | |
| TCCAAAGAGCTCAGATGTAA+CGG | - | chr5.2:17010104-17010123 | None:intergenic | 40.0% | |
| TGATTGACACTCAAACTGTC+GGG | - | chr5.2:17010127-17010146 | None:intergenic | 40.0% | |
| TGGGGATGTATATCTGTCAT+AGG | + | chr5.2:17010313-17010332 | MS.gene82144:CDS | 40.0% | |
| TTCTGGACATTTGTATGGTG+GGG | + | chr5.2:17010295-17010314 | MS.gene82144:CDS | 40.0% | |
| TTTCATATTTGCAGCTGCAG+TGG | + | chr5.2:17010078-17010097 | MS.gene82144:CDS | 40.0% | |
| ! | ATTTTGCCGTTATGCATTCC+TGG | + | chr5.2:17009319-17009338 | MS.gene82144:CDS | 40.0% |
| !!! | GTTTTTTGGGCAGGATCAAT+TGG | + | chr5.2:17009561-17009580 | MS.gene82144:CDS | 40.0% |
| !!! | TTGTGACAAGTTTTTTGGGC+AGG | + | chr5.2:17009552-17009571 | MS.gene82144:CDS | 40.0% |
| CAGCCATGAAACCATAGAGA+AGG | - | chr5.2:17010183-17010202 | None:intergenic | 45.0% | |
| CCAATTGGATCCCTTCTCTA+TGG | + | chr5.2:17010169-17010188 | MS.gene82144:CDS | 45.0% | |
| CCATAGAGAAGGGATCCAAT+TGG | - | chr5.2:17010172-17010191 | None:intergenic | 45.0% | |
| CCCAATTGCATTCATCTTGC+TGG | + | chr5.2:17009994-17010013 | MS.gene82144:CDS | 45.0% | |
| GCACTCACTGTGAGTTTCAA+TGG | + | chr5.2:17008995-17009014 | MS.gene82144:CDS | 45.0% | |
| GCAGGAAGTTCTACTTTGCA+AGG | + | chr5.2:17009939-17009958 | MS.gene82144:intron | 45.0% | |
| GCAGGATCAATTGGAAATGC+TGG | + | chr5.2:17009570-17009589 | MS.gene82144:CDS | 45.0% | |
| GCCAGCAAGATGAATGCAAT+TGG | - | chr5.2:17009998-17010017 | None:intergenic | 45.0% | |
| TGAGTGGTTGCAGTATCTGT+TGG | + | chr5.2:17008909-17008928 | MS.gene82144:CDS | 45.0% | |
| ! | CAACCTTTTCACAAGAACCG+CGG | - | chr5.2:17009611-17009630 | None:intergenic | 45.0% |
| ! | CAAGGTCTGCTTGGTGAAAA+CGG | + | chr5.2:17009493-17009512 | MS.gene82144:CDS | 45.0% |
| ! | CTGCTTGGTGAAAACGGATA+TGG | + | chr5.2:17009499-17009518 | MS.gene82144:CDS | 45.0% |
| ! | TACTTCTGCGGAGGTACAAT+TGG | + | chr5.2:17009655-17009674 | MS.gene82144:CDS | 45.0% |
| ! | TATAACCGAGTTCTGACGAG+CGG | - | chr5.2:17009182-17009201 | None:intergenic | 45.0% |
| !! | AGCTAGAGTTTTCGACTCGA+AGG | - | chr5.2:17009390-17009409 | None:intergenic | 45.0% |
| !! | GGCTTCATCTGAAAGTGCTA+TGG | + | chr5.2:17010015-17010034 | MS.gene82144:CDS | 45.0% |
| !! | TGGGGTTGGTAAAAGTGCAA+TGG | - | chr5.2:17009978-17009997 | None:intergenic | 45.0% |
| AAAGGCGGTTGGCGAAGAAT+CGG | - | chr5.2:17009136-17009155 | None:intergenic | 50.0% | |
| ACGTCGAGGCTTTACTTTGG+TGG | + | chr5.2:17009280-17009299 | MS.gene82144:CDS | 50.0% | |
| AGTCAGGCATAGCTGAAAGC+AGG | - | chr5.2:17009786-17009805 | None:intergenic | 50.0% | |
| CACAGTGAGTGCCAATGCTA+AGG | - | chr5.2:17008987-17009006 | None:intergenic | 50.0% | |
| CAGTCGGTTGCGCTGAAATA+CGG | - | chr5.2:17010402-17010421 | None:intergenic | 50.0% | |
| CGAAGTGAGAAGAGGGACAA+GGG | - | chr5.2:17009098-17009117 | None:intergenic | 50.0% | |
| CGCGGTTCTTGTGAAAAGGT+TGG | + | chr5.2:17009609-17009628 | MS.gene82144:CDS | 50.0% | |
| CTTCTGATGAAGCAACGTCG+AGG | + | chr5.2:17009266-17009285 | MS.gene82144:CDS | 50.0% | |
| GAGTCGACGTTCTATAACCG+TGG | - | chr5.2:17009726-17009745 | None:intergenic | 50.0% | |
| GAGTGTCTGATAAAGGCGGT+TGG | - | chr5.2:17009147-17009166 | None:intergenic | 50.0% | |
| GCAACGTCGAGGCTTTACTT+TGG | + | chr5.2:17009277-17009296 | MS.gene82144:CDS | 50.0% | |
| GCCGTTACATCTGAGCTCTT+TGG | + | chr5.2:17010100-17010119 | MS.gene82144:CDS | 50.0% | |
| GCTAATGCTATCTCAGCACC+TGG | + | chr5.2:17010217-17010236 | MS.gene82144:CDS | 50.0% | |
| GCTATCTCAGCACCTGGAAA+TGG | + | chr5.2:17010223-17010242 | MS.gene82144:CDS | 50.0% | |
| TCTACTTTGCAAGGACAGGG+TGG | + | chr5.2:17009948-17009967 | MS.gene82144:CDS | 50.0% | |
| TGAAAGCAGGCGTCCAAAGA+AGG | - | chr5.2:17009773-17009792 | None:intergenic | 50.0% | |
| !! | GAAGAGGGACAAGGGCATTT+AGG | - | chr5.2:17009090-17009109 | None:intergenic | 50.0% |
| !! | GAGTTTTCGACTCGAAGGCT+CGG | - | chr5.2:17009385-17009404 | None:intergenic | 50.0% |
| !! | TGGGACGGCAATGCTATTTC+TGG | + | chr5.2:17010278-17010297 | MS.gene82144:CDS | 50.0% |
| ACATGCCGCTCGTCAGAACT+CGG | + | chr5.2:17009174-17009193 | MS.gene82144:CDS | 55.0% | |
| CAGCCGCGGTTCTTGTGAAA+AGG | + | chr5.2:17009605-17009624 | MS.gene82144:CDS | 55.0% | |
| CGGAGCAATGGAGATGCTCA+AGG | + | chr5.2:17009475-17009494 | MS.gene82144:CDS | 55.0% | |
| CTGCGAGCGAAGTGAGAAGA+GGG | - | chr5.2:17009105-17009124 | None:intergenic | 55.0% | |
| GAAGGCTCGGATGAACAGCA+CGG | - | chr5.2:17009372-17009391 | None:intergenic | 55.0% | |
| GCACTAATCGGTCTGAGCTC+AGG | + | chr5.2:17010055-17010074 | MS.gene82144:CDS | 55.0% | |
| GCGAAGTGAGAAGAGGGACA+AGG | - | chr5.2:17009099-17009118 | None:intergenic | 55.0% | |
| GGAGATGCTCAAGGTCTGCT+TGG | + | chr5.2:17009484-17009503 | MS.gene82144:CDS | 55.0% | |
| GGGAGAAGAACATTCAGCCG+CGG | + | chr5.2:17009591-17009610 | MS.gene82144:CDS | 55.0% | |
| TTACGCGTCAAAGCAGCGCT+CGG | + | chr5.2:17009455-17009474 | MS.gene82144:CDS | 55.0% | |
| CAAAGCAGCGCTCGGAGCAA+TGG | + | chr5.2:17009463-17009482 | MS.gene82144:CDS | 60.0% | |
| GCTGCGAGCGAAGTGAGAAG+AGG | - | chr5.2:17009106-17009125 | None:intergenic | 60.0% | |
| ! | GAGCGCTGCTTTGACGCGTA+AGG | - | chr5.2:17009456-17009475 | None:intergenic | 60.0% |
| ! | GAGTTCTGACGAGCGGCATG+TGG | - | chr5.2:17009175-17009194 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 17008787 | 17010429 | 17008787 | ID=MS.gene82144 |
| chr5.2 | mRNA | 17008787 | 17010429 | 17008787 | ID=MS.gene82144.t1;Parent=MS.gene82144 |
| chr5.2 | exon | 17008787 | 17008801 | 17008787 | ID=MS.gene82144.t1.exon1;Parent=MS.gene82144.t1 |
| chr5.2 | CDS | 17008787 | 17008801 | 17008787 | ID=cds.MS.gene82144.t1;Parent=MS.gene82144.t1 |
| chr5.2 | exon | 17008890 | 17009815 | 17008890 | ID=MS.gene82144.t1.exon2;Parent=MS.gene82144.t1 |
| chr5.2 | CDS | 17008890 | 17009815 | 17008890 | ID=cds.MS.gene82144.t1;Parent=MS.gene82144.t1 |
| chr5.2 | exon | 17009943 | 17010429 | 17009943 | ID=MS.gene82144.t1.exon3;Parent=MS.gene82144.t1 |
| chr5.2 | CDS | 17009943 | 17010429 | 17009943 | ID=cds.MS.gene82144.t1;Parent=MS.gene82144.t1 |
| Gene Sequence |
| Protein sequence |
