Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82358.t1 | XP_003607784.1 | 90.1 | 81 | 8 | 0 | 1 | 81 | 1 | 81 | 3.60E-13 | 84 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82358.t1 | G7JHG4 | 90.1 | 81 | 8 | 0 | 1 | 81 | 1 | 81 | 2.6e-13 | 84.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene82358 | 0.804378 | 2.22E-49 | -1.69E-46 |
| MS.gene049151 | MS.gene82358 | 0.816674 | 4.90E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene82358 | 0.808455 | 3.07E-50 | -1.69E-46 |
| MS.gene049346 | MS.gene82358 | 0.81106 | 8.46E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene82358 | 0.808623 | 2.83E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene82358 | 0.842858 | 2.00E-58 | -1.69E-46 |
| MS.gene049910 | MS.gene82358 | 0.825214 | 5.29E-54 | -1.69E-46 |
| MS.gene049915 | MS.gene82358 | 0.806233 | 9.09E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene82358 | 0.832086 | 1.15E-55 | -1.69E-46 |
| MS.gene050404 | MS.gene82358 | 0.811702 | 6.14E-51 | -1.69E-46 |
| MS.gene050406 | MS.gene82358 | 0.83221 | 1.08E-55 | -1.69E-46 |
| MS.gene050426 | MS.gene82358 | -0.824441 | 8.06E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene82358 | 0.815159 | 1.07E-51 | -1.69E-46 |
| MS.gene050529 | MS.gene82358 | 0.827641 | 1.40E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene82358 | -0.823208 | 1.57E-53 | -1.69E-46 |
| MS.gene050642 | MS.gene82358 | 0.842981 | 1.86E-58 | -1.69E-46 |
| MS.gene050971 | MS.gene82358 | 0.802574 | 5.26E-49 | -1.69E-46 |
| MS.gene051535 | MS.gene82358 | 0.801018 | 1.10E-48 | -1.69E-46 |
| MS.gene051860 | MS.gene82358 | 0.81592 | 7.22E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene82358 | 0.85518 | 7.50E-62 | -1.69E-46 |
| MS.gene052077 | MS.gene82358 | 0.893375 | 6.67E-75 | -1.69E-46 |
| MS.gene052135 | MS.gene82358 | 0.805229 | 1.48E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene82358 | 0.831345 | 1.76E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene82358 | 0.82728 | 1.71E-54 | -1.69E-46 |
| MS.gene052310 | MS.gene82358 | 0.815939 | 7.15E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene82358 | 0.870826 | 1.09E-66 | -1.69E-46 |
| MS.gene053073 | MS.gene82358 | 0.83199 | 1.22E-55 | -1.69E-46 |
| MS.gene05316 | MS.gene82358 | 0.801463 | 8.90E-49 | -1.69E-46 |
| MS.gene053494 | MS.gene82358 | 0.816011 | 6.89E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene82358 | 0.855176 | 7.52E-62 | -1.69E-46 |
| MS.gene053836 | MS.gene82358 | 0.835961 | 1.24E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene82358 | 0.855963 | 4.43E-62 | -1.69E-46 |
| MS.gene054379 | MS.gene82358 | 0.810094 | 1.37E-50 | -1.69E-46 |
| MS.gene054542 | MS.gene82358 | 0.828908 | 6.92E-55 | -1.69E-46 |
| MS.gene054930 | MS.gene82358 | 0.830911 | 2.25E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene82358 | 0.823295 | 1.50E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene82358 | 0.808256 | 3.39E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene82358 | 0.835459 | 1.66E-56 | -1.69E-46 |
| MS.gene055701 | MS.gene82358 | 0.809613 | 1.74E-50 | -1.69E-46 |
| MS.gene056447 | MS.gene82358 | 0.812617 | 3.87E-51 | -1.69E-46 |
| MS.gene056612 | MS.gene82358 | 0.824141 | 9.48E-54 | -1.69E-46 |
| MS.gene056659 | MS.gene82358 | 0.809552 | 1.79E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene82358 | 0.857016 | 2.17E-62 | -1.69E-46 |
| MS.gene05702 | MS.gene82358 | 0.841001 | 6.19E-58 | -1.69E-46 |
| MS.gene057396 | MS.gene82358 | 0.805371 | 1.38E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene82358 | 0.816859 | 4.45E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene82358 | 0.803232 | 3.85E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene82358 | 0.804827 | 1.79E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene82358 | 0.815073 | 1.11E-51 | -1.69E-46 |
| MS.gene058435 | MS.gene82358 | 0.806331 | 8.66E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene82358 | 0.829837 | 4.12E-55 | -1.69E-46 |
| MS.gene058845 | MS.gene82358 | 0.80828 | 3.35E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene82358 | 0.811944 | 5.43E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene82358 | 0.817031 | 4.07E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene82358 | 0.84817 | 7.27E-60 | -1.69E-46 |
| MS.gene059294 | MS.gene82358 | 0.811419 | 7.07E-51 | -1.69E-46 |
| MS.gene059511 | MS.gene82358 | 0.829966 | 3.83E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene82358 | 0.803247 | 3.82E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene82358 | 0.831024 | 2.11E-55 | -1.69E-46 |
| MS.gene059894 | MS.gene82358 | 0.870157 | 1.80E-66 | -1.69E-46 |
| MS.gene060084 | MS.gene82358 | 0.80422 | 2.40E-49 | -1.69E-46 |
| MS.gene060940 | MS.gene82358 | 0.833832 | 4.25E-56 | -1.69E-46 |
| MS.gene060942 | MS.gene82358 | 0.808177 | 3.52E-50 | -1.69E-46 |
| MS.gene060943 | MS.gene82358 | 0.811325 | 7.41E-51 | -1.69E-46 |
| MS.gene061002 | MS.gene82358 | 0.828355 | 9.42E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene82358 | 0.870475 | 1.42E-66 | -1.69E-46 |
| MS.gene061159 | MS.gene82358 | 0.8322 | 1.08E-55 | -1.69E-46 |
| MS.gene061265 | MS.gene82358 | 0.800775 | 1.23E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82358.t1 | MTR_4g082900 | 83.951 | 81 | 13 | 0 | 1 | 81 | 1 | 81 | 6.92e-36 | 116 |
| MS.gene82358.t1 | MTR_4g082883 | 58.108 | 74 | 31 | 0 | 8 | 81 | 26 | 99 | 8.39e-21 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGACCTAACGATCTTT+TGG | 0.317659 | 4.2:-26773818 | MS.gene82358:CDS |
| TTTGCCAATAGGATCTGGTT+TGG | 0.364938 | 4.2:+26773651 | None:intergenic |
| TTGCCAATAGGATCTGGTTT+GGG | 0.376061 | 4.2:+26773652 | None:intergenic |
| AGCATCATCAACCGCACCTT+TGG | 0.404914 | 4.2:+26773627 | None:intergenic |
| GTACTTGCCAAAAGATCGTT+AGG | 0.439536 | 4.2:+26773811 | None:intergenic |
| AAACCCAAACCAGATCCTAT+TGG | 0.473112 | 4.2:-26773655 | MS.gene82358:CDS |
| GATGATGCTACAAAGTCTGC+TGG | 0.547087 | 4.2:-26773613 | MS.gene82358:CDS |
| ACTGTAGGAAATCTTGCAGC+TGG | 0.550456 | 4.2:-26773760 | MS.gene82358:CDS |
| GCTGGTGTTCATAAGACTGT+AGG | 0.573068 | 4.2:-26773709 | MS.gene82358:CDS |
| GCATCAGCACAGAAAACTGT+AGG | 0.588939 | 4.2:-26773775 | MS.gene82358:CDS |
| GTAGGAAATCTTGCAGCTGG+TGG | 0.593110 | 4.2:-26773757 | MS.gene82358:CDS |
| GATCCTATTGGCAAAACCAA+AGG | 0.604323 | 4.2:-26773643 | MS.gene82358:CDS |
| AATGTGGGAAATGCTGCAGC+TGG | 0.619200 | 4.2:-26773727 | MS.gene82358:CDS |
| TATTGGCAAAACCAAAGGTG+CGG | 0.679241 | 4.2:-26773638 | MS.gene82358:CDS |
| GATGCTACAAAGTCTGCTGG+AGG | 0.702868 | 4.2:-26773610 | MS.gene82358:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTACAAAATCAGCAGCGT+TGG | + | chr4.2:26773745-26773764 | None:intergenic | 35.0% |
| AAACCCAAACCAGATCCTAT+TGG | - | chr4.2:26773762-26773781 | MS.gene82358:CDS | 40.0% | |
| GATCCTATTGGCAAAACCAA+AGG | - | chr4.2:26773774-26773793 | MS.gene82358:CDS | 40.0% | |
| GTACTTGCCAAAAGATCGTT+AGG | + | chr4.2:26773609-26773628 | None:intergenic | 40.0% | |
| TATTGGCAAAACCAAAGGTG+CGG | - | chr4.2:26773779-26773798 | MS.gene82358:CDS | 40.0% | |
| TTTGCCAATAGGATCTGGTT+TGG | + | chr4.2:26773769-26773788 | None:intergenic | 40.0% | |
| ! | CAGCATTTCCCACATTTTTC+TGG | + | chr4.2:26773686-26773705 | None:intergenic | 40.0% |
| ! | CGATCTTTTGGCAAGTACAA+AGG | - | chr4.2:26773611-26773630 | MS.gene82358:CDS | 40.0% |
| ! | TTGCCAATAGGATCTGGTTT+GGG | + | chr4.2:26773768-26773787 | None:intergenic | 40.0% |
| !!! | TTGGTTTTGCCAATAGGATC+TGG | + | chr4.2:26773774-26773793 | None:intergenic | 40.0% |
| ACTGTAGGAAATCTTGCAGC+TGG | - | chr4.2:26773657-26773676 | MS.gene82358:CDS | 45.0% | |
| GATGATGCTACAAAGTCTGC+TGG | - | chr4.2:26773804-26773823 | MS.gene82358:CDS | 45.0% | |
| GCATCAGCACAGAAAACTGT+AGG | - | chr4.2:26773642-26773661 | MS.gene82358:CDS | 45.0% | |
| GCTGGTGTTCATAAGACTGT+AGG | - | chr4.2:26773708-26773727 | MS.gene82358:CDS | 45.0% | |
| !!! | GCACCTTTGGTTTTGCCAAT+AGG | + | chr4.2:26773780-26773799 | None:intergenic | 45.0% |
| AATGTGGGAAATGCTGCAGC+TGG | - | chr4.2:26773690-26773709 | MS.gene82358:CDS | 50.0% | |
| AGCATCATCAACCGCACCTT+TGG | + | chr4.2:26773793-26773812 | None:intergenic | 50.0% | |
| AGCTGGTGGCCAGAAAAATG+TGG | - | chr4.2:26773674-26773693 | MS.gene82358:CDS | 50.0% | |
| GATGCTACAAAGTCTGCTGG+AGG | - | chr4.2:26773807-26773826 | MS.gene82358:CDS | 50.0% | |
| GCTGGTGGCCAGAAAAATGT+GGG | - | chr4.2:26773675-26773694 | MS.gene82358:CDS | 50.0% | |
| GTAGGAAATCTTGCAGCTGG+TGG | - | chr4.2:26773660-26773679 | MS.gene82358:CDS | 50.0% | |
| ! | GGCTGGACCTAACGATCTTT+TGG | - | chr4.2:26773599-26773618 | MS.gene82358:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26773597 | 26773842 | 26773597 | ID=MS.gene82358 |
| chr4.2 | mRNA | 26773597 | 26773842 | 26773597 | ID=MS.gene82358.t1;Parent=MS.gene82358 |
| chr4.2 | exon | 26773597 | 26773842 | 26773597 | ID=MS.gene82358.t1.exon1;Parent=MS.gene82358.t1 |
| chr4.2 | CDS | 26773597 | 26773842 | 26773597 | ID=cds.MS.gene82358.t1;Parent=MS.gene82358.t1 |
| Gene Sequence |
| Protein sequence |
