Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84712.t1 | XP_003601907.1 | 94.5 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 7.90E-168 | 599.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84712.t1 | Q8L936 | 49.0 | 345 | 149 | 8 | 6 | 327 | 9 | 349 | 2.2e-73 | 277.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84712.t1 | G7J365 | 94.5 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 5.7e-168 | 599.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene84712 | 0.823997 | 1.02E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene84712 | -0.801127 | 1.04E-48 | -1.69E-46 |
| MS.gene049285 | MS.gene84712 | -0.814936 | 1.20E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene84712 | -0.834178 | 3.48E-56 | -1.69E-46 |
| MS.gene049483 | MS.gene84712 | 0.81253 | 4.05E-51 | -1.69E-46 |
| MS.gene049634 | MS.gene84712 | 0.812868 | 3.41E-51 | -1.69E-46 |
| MS.gene04986 | MS.gene84712 | 0.843147 | 1.68E-58 | -1.69E-46 |
| MS.gene05010 | MS.gene84712 | -0.829618 | 4.65E-55 | -1.69E-46 |
| MS.gene050163 | MS.gene84712 | -0.806482 | 8.05E-50 | -1.69E-46 |
| MS.gene050165 | MS.gene84712 | -0.828037 | 1.12E-54 | -1.69E-46 |
| MS.gene050173 | MS.gene84712 | 0.828929 | 6.84E-55 | -1.69E-46 |
| MS.gene050216 | MS.gene84712 | -0.83964 | 1.40E-57 | -1.69E-46 |
| MS.gene050333 | MS.gene84712 | -0.826286 | 2.95E-54 | -1.69E-46 |
| MS.gene050381 | MS.gene84712 | 0.811764 | 5.95E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene84712 | -0.802206 | 6.26E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene84712 | -0.833055 | 6.64E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene84712 | -0.815704 | 8.07E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene84712 | -0.853087 | 3.01E-61 | -1.69E-46 |
| MS.gene050675 | MS.gene84712 | -0.802126 | 6.50E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene84712 | -0.803018 | 4.26E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene84712 | -0.801639 | 8.19E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene84712 | 0.802872 | 4.56E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene84712 | -0.820975 | 5.16E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene84712 | 0.804161 | 2.47E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene84712 | 0.80629 | 8.84E-50 | -1.69E-46 |
| MS.gene051364 | MS.gene84712 | 0.841979 | 3.42E-58 | -1.69E-46 |
| MS.gene051366 | MS.gene84712 | 0.853681 | 2.04E-61 | -1.69E-46 |
| MS.gene051367 | MS.gene84712 | 0.84741 | 1.18E-59 | -1.69E-46 |
| MS.gene051810 | MS.gene84712 | -0.81642 | 5.58E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene84712 | 0.829614 | 4.66E-55 | -1.69E-46 |
| MS.gene053561 | MS.gene84712 | 0.87038 | 1.52E-66 | -1.69E-46 |
| MS.gene054056 | MS.gene84712 | 0.84168 | 4.10E-58 | -1.69E-46 |
| MS.gene054057 | MS.gene84712 | 0.808578 | 2.89E-50 | -1.69E-46 |
| MS.gene054616 | MS.gene84712 | 0.844295 | 8.26E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene84712 | -0.821784 | 3.36E-53 | -1.69E-46 |
| MS.gene054708 | MS.gene84712 | 0.819636 | 1.05E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene84712 | -0.832161 | 1.11E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene84712 | 0.849455 | 3.20E-60 | -1.69E-46 |
| MS.gene055202 | MS.gene84712 | 0.81644 | 5.53E-52 | -1.69E-46 |
| MS.gene055204 | MS.gene84712 | 0.801066 | 1.07E-48 | -1.69E-46 |
| MS.gene055362 | MS.gene84712 | -0.827098 | 1.89E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene84712 | -0.807301 | 5.40E-50 | -1.69E-46 |
| MS.gene055634 | MS.gene84712 | -0.843649 | 1.23E-58 | -1.69E-46 |
| MS.gene055638 | MS.gene84712 | -0.808971 | 2.38E-50 | -1.69E-46 |
| MS.gene055776 | MS.gene84712 | -0.853296 | 2.63E-61 | -1.69E-46 |
| MS.gene055942 | MS.gene84712 | 0.857504 | 1.56E-62 | -1.69E-46 |
| MS.gene056185 | MS.gene84712 | 0.838804 | 2.31E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene84712 | -0.842286 | 2.84E-58 | -1.69E-46 |
| MS.gene056550 | MS.gene84712 | 0.823844 | 1.11E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene84712 | -0.824451 | 8.01E-54 | -1.69E-46 |
| MS.gene056761 | MS.gene84712 | 0.833288 | 5.81E-56 | -1.69E-46 |
| MS.gene057014 | MS.gene84712 | 0.809874 | 1.53E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene84712 | 0.820026 | 8.52E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene84712 | 0.815363 | 9.61E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene84712 | -0.808255 | 3.39E-50 | -1.69E-46 |
| MS.gene058493 | MS.gene84712 | 0.813713 | 2.23E-51 | -1.69E-46 |
| MS.gene058494 | MS.gene84712 | 0.805559 | 1.26E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene84712 | -0.832744 | 7.94E-56 | -1.69E-46 |
| MS.gene05884 | MS.gene84712 | 0.819921 | 9.01E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene84712 | -0.849636 | 2.85E-60 | -1.69E-46 |
| MS.gene059677 | MS.gene84712 | -0.833904 | 4.08E-56 | -1.69E-46 |
| MS.gene059690 | MS.gene84712 | 0.813552 | 2.42E-51 | -1.69E-46 |
| MS.gene059694 | MS.gene84712 | 0.810397 | 1.18E-50 | -1.69E-46 |
| MS.gene059697 | MS.gene84712 | 0.801609 | 8.30E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene84712 | -0.842598 | 2.34E-58 | -1.69E-46 |
| MS.gene060337 | MS.gene84712 | 0.837272 | 5.73E-57 | -1.69E-46 |
| MS.gene060703 | MS.gene84712 | -0.842935 | 1.91E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene84712 | -0.824787 | 6.68E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene84712 | -0.81114 | 8.12E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84712.t1 | MTR_3g086670 | 94.495 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 0.0 | 634 |
| MS.gene84712.t1 | MTR_8g089850 | 62.462 | 325 | 112 | 6 | 7 | 328 | 9 | 326 | 2.38e-131 | 377 |
| MS.gene84712.t1 | MTR_3g099670 | 36.237 | 287 | 172 | 4 | 2 | 279 | 5 | 289 | 1.26e-55 | 184 |
| MS.gene84712.t1 | MTR_4g115420 | 26.875 | 320 | 204 | 12 | 4 | 306 | 9 | 315 | 3.14e-17 | 82.8 |
| MS.gene84712.t1 | MTR_3g106900 | 34.014 | 147 | 92 | 3 | 4 | 147 | 6 | 150 | 2.65e-16 | 80.1 |
| MS.gene84712.t1 | MTR_2g037980 | 29.902 | 204 | 125 | 6 | 4 | 192 | 7 | 207 | 4.05e-16 | 79.3 |
| MS.gene84712.t1 | MTR_4g127440 | 28.571 | 196 | 122 | 5 | 5 | 185 | 8 | 200 | 5.62e-16 | 79.0 |
| MS.gene84712.t1 | MTR_8g008860 | 35.135 | 148 | 90 | 3 | 4 | 147 | 6 | 151 | 9.86e-15 | 75.1 |
| MS.gene84712.t1 | MTR_2g008170 | 33.108 | 148 | 93 | 3 | 4 | 147 | 6 | 151 | 3.06e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84712.t1 | AT4G40080 | 48.286 | 350 | 154 | 8 | 1 | 327 | 4 | 349 | 3.14e-100 | 300 |
| MS.gene84712.t1 | AT5G10410 | 31.875 | 320 | 182 | 9 | 7 | 301 | 8 | 316 | 1.36e-34 | 129 |
| MS.gene84712.t1 | AT5G65370 | 31.046 | 306 | 173 | 9 | 1 | 280 | 1 | 294 | 1.23e-31 | 120 |
| MS.gene84712.t1 | AT5G57200 | 30.049 | 203 | 125 | 6 | 4 | 192 | 7 | 206 | 9.80e-17 | 81.3 |
| MS.gene84712.t1 | AT4G25940 | 28.641 | 206 | 123 | 6 | 1 | 185 | 4 | 206 | 1.05e-16 | 81.3 |
| MS.gene84712.t1 | AT2G01600 | 30.667 | 225 | 137 | 8 | 1 | 210 | 4 | 224 | 8.50e-16 | 78.6 |
| MS.gene84712.t1 | AT4G02650 | 34.000 | 150 | 92 | 3 | 3 | 147 | 5 | 152 | 1.90e-14 | 74.3 |
| MS.gene84712.t1 | AT2G25430 | 31.973 | 147 | 95 | 3 | 4 | 147 | 5 | 149 | 5.23e-14 | 73.2 |
| MS.gene84712.t1 | AT1G14910 | 27.675 | 271 | 166 | 11 | 31 | 284 | 36 | 293 | 6.22e-14 | 73.2 |
| MS.gene84712.t1 | AT5G35200 | 29.947 | 187 | 121 | 6 | 4 | 185 | 11 | 192 | 1.09e-13 | 72.0 |
| MS.gene84712.t1 | AT5G35200 | 29.947 | 187 | 121 | 6 | 4 | 185 | 11 | 192 | 1.09e-13 | 72.0 |
| MS.gene84712.t1 | AT4G32285 | 31.293 | 147 | 96 | 3 | 4 | 147 | 5 | 149 | 1.33e-13 | 72.0 |
| MS.gene84712.t1 | AT4G32285 | 31.293 | 147 | 96 | 3 | 4 | 147 | 5 | 149 | 1.33e-13 | 72.0 |
| MS.gene84712.t1 | AT1G03050 | 32.667 | 150 | 94 | 3 | 3 | 147 | 5 | 152 | 6.72e-13 | 69.7 |
Find 95 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACGGCGATTAAACATTTA+AGG | 0.163434 | 3.4:+80750640 | None:intergenic |
| TTGGTTAGAGTTAAAGAATT+TGG | 0.219230 | 3.4:-80750222 | MS.gene84712:CDS |
| TGGTTAGAGTTAAAGAATTT+GGG | 0.246190 | 3.4:-80750221 | MS.gene84712:CDS |
| GTCAAGAGGTGTTTGTGTTT+TGG | 0.268343 | 3.4:+80750759 | None:intergenic |
| AAACTTGGTTGTCTTGGTTT+TGG | 0.268735 | 3.4:-80750189 | MS.gene84712:CDS |
| TGATTCTTTGTTGGCCTTGA+TGG | 0.273576 | 3.4:-80750352 | MS.gene84712:CDS |
| CAAGGTGATTGTTGAGATAA+TGG | 0.276764 | 3.4:-80750286 | MS.gene84712:CDS |
| GGAGAAGAAATTCTGGGATT+TGG | 0.296571 | 3.4:-80750085 | MS.gene84712:CDS |
| CTGTGTGCTTCTAGAACTCT+TGG | 0.303930 | 3.4:-80750447 | MS.gene84712:CDS |
| TGGTTGAGGAGAAGAAATTC+TGG | 0.306064 | 3.4:-80750092 | MS.gene84712:CDS |
| TATCTTGGGAACTCTCTTCT+TGG | 0.310691 | 3.4:-80750503 | MS.gene84712:CDS |
| AGAACTCTTGGTTTCTTCCT+TGG | 0.315271 | 3.4:-80750435 | MS.gene84712:CDS |
| CTCTTGACTCTTCTTTCCTC+TGG | 0.318672 | 3.4:-80750744 | MS.gene84712:CDS |
| CCATTAGGTTTCCTTCTTCA+AGG | 0.324011 | 3.4:-80749930 | MS.gene84712:CDS |
| TGTTCACTCATTGGAGTATT+AGG | 0.324515 | 3.4:+80750312 | None:intergenic |
| TGGGAGGATGAAGGTGTATA+GGG | 0.331362 | 3.4:-80750040 | MS.gene84712:CDS |
| ACAAAACAAGTTCAATATCT+TGG | 0.345547 | 3.4:-80750518 | MS.gene84712:CDS |
| TTCTCTTAACAGATCACTAT+TGG | 0.348060 | 3.4:+80750377 | None:intergenic |
| TATGGTGATGAATGAAGTTT+TGG | 0.357854 | 3.4:-80750241 | MS.gene84712:CDS |
| ATCTTGGGAACTCTCTTCTT+GGG | 0.358410 | 3.4:-80750502 | MS.gene84712:CDS |
| TTAGGTCTGTTGATTCCATT+AGG | 0.360896 | 3.4:-80749945 | MS.gene84712:CDS |
| ACCTTGTTCTGTTCACTCAT+TGG | 0.361918 | 3.4:+80750303 | None:intergenic |
| GGTGGAAGATGATGGGGTTA+TGG | 0.363211 | 3.4:-80750259 | MS.gene84712:CDS |
| TTGGGAGGATGAAGGTGTAT+AGG | 0.379157 | 3.4:-80750041 | MS.gene84712:CDS |
| GATTGTTGAGATAATGGATT+TGG | 0.380186 | 3.4:-80750280 | MS.gene84712:CDS |
| AAGAGAAACTGATTCTTTGT+TGG | 0.385719 | 3.4:-80750361 | MS.gene84712:CDS |
| AGTTAAAGAATTTGGGGAGA+GGG | 0.391874 | 3.4:-80750214 | MS.gene84712:CDS |
| ATGAAGGTGTATAGGGAAGA+AGG | 0.403447 | 3.4:-80750033 | MS.gene84712:CDS |
| TTGGCCTTGATGGAAGGAAT+TGG | 0.410057 | 3.4:-80750342 | MS.gene84712:CDS |
| AGGGAGAAACTTGGTTGTCT+TGG | 0.426496 | 3.4:-80750195 | MS.gene84712:CDS |
| CTCTGAAGTTTGAGAGGTTG+AGG | 0.434153 | 3.4:+80750541 | None:intergenic |
| GGTTTGATGACAGAGTTCTT+AGG | 0.440749 | 3.4:-80749963 | MS.gene84712:CDS |
| CTGCTGAGTTGTGAGTGGTT+TGG | 0.441669 | 3.4:+80750667 | None:intergenic |
| ACCACCAGTGTAAGGGTAAA+CGG | 0.442204 | 3.4:+80750572 | None:intergenic |
| TGGATTTGGTGGAAGATGAT+GGG | 0.446048 | 3.4:-80750266 | MS.gene84712:CDS |
| CAAAACAAGTTCAATATCTT+GGG | 0.446855 | 3.4:-80750517 | MS.gene84712:CDS |
| TAGTTTCTACCACCAGTGTA+AGG | 0.447417 | 3.4:+80750564 | None:intergenic |
| AACTCTCTTCTTGGGTTCGA+TGG | 0.450996 | 3.4:-80750494 | MS.gene84712:CDS |
| AAGAAACTGAAAGAGGTGAT+AGG | 0.455772 | 3.4:-80750882 | MS.gene84712:CDS |
| AACGGCGATTAAACATTTAA+GGG | 0.462073 | 3.4:+80750641 | None:intergenic |
| CCTTGAAGAAGGAAACCTAA+TGG | 0.462290 | 3.4:+80749930 | None:intergenic |
| AGAGAGAATGATGATGATGA+TGG | 0.463274 | 3.4:-80750118 | MS.gene84712:CDS |
| TTTGGGGAGAGGGAGAAACT+TGG | 0.467834 | 3.4:-80750204 | MS.gene84712:CDS |
| AGAGAATTGAAGGAAAAGGT+TGG | 0.470401 | 3.4:-80750060 | MS.gene84712:CDS |
| GATGATGATGGAGGTGGTTG+AGG | 0.470627 | 3.4:-80750106 | MS.gene84712:CDS |
| CTCTGCGGTGGAGCTTCTCA+TGG | 0.472704 | 3.4:-80750697 | MS.gene84712:CDS |
| GGATTTGGTGAGAGAATTGA+AGG | 0.473257 | 3.4:-80750070 | MS.gene84712:CDS |
| ATGGATTTGGTGGAAGATGA+TGG | 0.474899 | 3.4:-80750267 | MS.gene84712:CDS |
| GAGTTAAAGAATTTGGGGAG+AGG | 0.480788 | 3.4:-80750215 | MS.gene84712:CDS |
| TGGCCTTGATGGAAGGAATT+GGG | 0.482108 | 3.4:-80750341 | MS.gene84712:CDS |
| TGAAGGTGTATAGGGAAGAA+GGG | 0.482642 | 3.4:-80750032 | MS.gene84712:CDS |
| GGTGAGAGAATTGAAGGAAA+AGG | 0.483133 | 3.4:-80750064 | MS.gene84712:CDS |
| AAGCTCCACCGCAGAGGATG+CGG | 0.484245 | 3.4:+80750704 | None:intergenic |
| ACTCTTCTTTCCTCTGGAGA+CGG | 0.488107 | 3.4:-80750738 | MS.gene84712:CDS |
| GAGAATTGAAGGAAAAGGTT+GGG | 0.493649 | 3.4:-80750059 | MS.gene84712:CDS |
| TTTCCCAATTCCTTCCATCA+AGG | 0.501907 | 3.4:+80750338 | None:intergenic |
| GGTTGAGGAGAAGAAATTCT+GGG | 0.518899 | 3.4:-80750091 | MS.gene84712:CDS |
| ATTTACAAGAACCTTGAAGA+AGG | 0.539232 | 3.4:+80749919 | None:intergenic |
| AGAGGTGATAGGATTAATGA+AGG | 0.539540 | 3.4:-80750871 | MS.gene84712:CDS |
| TGTTGAGATAATGGATTTGG+TGG | 0.546687 | 3.4:-80750277 | MS.gene84712:CDS |
| GGTGAAACGACGTCAGAGAA+AGG | 0.547020 | 3.4:-80750414 | MS.gene84712:CDS |
| GTCATCAAACCGATCAGACT+CGG | 0.547520 | 3.4:+80749975 | None:intergenic |
| GGATTTGGTGGAAGATGATG+GGG | 0.552285 | 3.4:-80750265 | MS.gene84712:CDS |
| GGAAAAGGTTGGGAGGATGA+AGG | 0.554877 | 3.4:-80750049 | MS.gene84712:CDS |
| GAGCTGATCACGGAGGATGA+AGG | 0.556396 | 3.4:+80750596 | None:intergenic |
| AGAAGGGAGGCTTAGCAGTA+CGG | 0.556866 | 3.4:-80750016 | MS.gene84712:CDS |
| AGTTGTGAGTGGTTTGGAGA+CGG | 0.568196 | 3.4:+80750673 | None:intergenic |
| TCTTTGTTGGCCTTGATGGA+AGG | 0.570838 | 3.4:-80750348 | MS.gene84712:CDS |
| ACAGAAGAACCGAGTCTGAT+CGG | 0.577525 | 3.4:-80749984 | MS.gene84712:CDS |
| TTCAATGAAGAAACTGAAAG+AGG | 0.579832 | 3.4:-80750889 | None:intergenic |
| TCCAATGAGTGAACAGAACA+AGG | 0.581482 | 3.4:-80750304 | MS.gene84712:CDS |
| TGCCACCGCATCCTCTGCGG+TGG | 0.581913 | 3.4:-80750709 | MS.gene84712:CDS |
| TCCGTTTACCCTTACACTGG+TGG | 0.585451 | 3.4:-80750573 | MS.gene84712:CDS |
| GAGTTCTAGAAGCACACAGA+AGG | 0.587402 | 3.4:+80750451 | None:intergenic |
| CTCCACCGCAGAGGATGCGG+TGG | 0.587882 | 3.4:+80750707 | None:intergenic |
| GGCTACTGCTGAGTTGTGAG+TGG | 0.589218 | 3.4:+80750662 | None:intergenic |
| GGTTAGAGTTAAAGAATTTG+GGG | 0.590495 | 3.4:-80750220 | MS.gene84712:CDS |
| CATGTGTTGTGGCACGTAGG+AGG | 0.596737 | 3.4:+80750796 | None:intergenic |
| AATTGAAGGAAAAGGTTGGG+AGG | 0.600206 | 3.4:-80750056 | MS.gene84712:CDS |
| CTCTCCGTTTACCCTTACAC+TGG | 0.601794 | 3.4:-80750576 | MS.gene84712:CDS |
| TTCTTGGGTTCGATGGTACG+CGG | 0.608953 | 3.4:-80750487 | MS.gene84712:CDS |
| AGTTTCTACCACCAGTGTAA+GGG | 0.622840 | 3.4:+80750565 | None:intergenic |
| AGGTACCGTGTTTGATGATG+TGG | 0.633958 | 3.4:+80750616 | None:intergenic |
| GGCACGTGAACCGTCTCCAG+AGG | 0.642214 | 3.4:+80750728 | None:intergenic |
| AGGTGTATAGGGAAGAAGGG+AGG | 0.646656 | 3.4:-80750029 | MS.gene84712:CDS |
| CATGAGAAGCTCCACCGCAG+AGG | 0.658489 | 3.4:+80750698 | None:intergenic |
| CAGAGGAAAGAAGAGTCAAG+AGG | 0.661118 | 3.4:+80750745 | None:intergenic |
| GTTGAAAGAGTCATGTGTTG+TGG | 0.662998 | 3.4:+80750785 | None:intergenic |
| AGTCATGTGTTGTGGCACGT+AGG | 0.663095 | 3.4:+80750793 | None:intergenic |
| GGTAAACGGAGAGCTGATCA+CGG | 0.664071 | 3.4:+80750586 | None:intergenic |
| GTGTTTGATGATGTGGTGAA+CGG | 0.664912 | 3.4:+80750623 | None:intergenic |
| ACGTGCCACCGCATCCTCTG+CGG | 0.680106 | 3.4:-80750712 | MS.gene84712:CDS |
| TCTCTGACGTCGTTTCACCA+AGG | 0.687518 | 3.4:+80750418 | None:intergenic |
| GTTCACCACATCATCAAACA+CGG | 0.734907 | 3.4:-80750621 | MS.gene84712:CDS |
| AAACGGAGAGCTGATCACGG+AGG | 0.785713 | 3.4:+80750589 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAACAAGTTCAATATCT+TGG | - | chr3.4:80750288-80750307 | MS.gene84712:CDS | 25.0% |
| ! | CAAAACAAGTTCAATATCTT+GGG | - | chr3.4:80750289-80750308 | MS.gene84712:CDS | 25.0% |
| ! | TGGTTAGAGTTAAAGAATTT+GGG | - | chr3.4:80750585-80750604 | MS.gene84712:CDS | 25.0% |
| ! | TTGGTTAGAGTTAAAGAATT+TGG | - | chr3.4:80750584-80750603 | MS.gene84712:CDS | 25.0% |
| AACGGCGATTAAACATTTAA+GGG | + | chr3.4:80750168-80750187 | None:intergenic | 30.0% | |
| GGTTAGAGTTAAAGAATTTG+GGG | - | chr3.4:80750586-80750605 | MS.gene84712:CDS | 30.0% | |
| TTCTCTTAACAGATCACTAT+TGG | + | chr3.4:80750432-80750451 | None:intergenic | 30.0% | |
| ! | AAGAGAAACTGATTCTTTGT+TGG | - | chr3.4:80750445-80750464 | MS.gene84712:CDS | 30.0% |
| !! | GATTGTTGAGATAATGGATT+TGG | - | chr3.4:80750526-80750545 | MS.gene84712:CDS | 30.0% |
| !! | TATGGTGATGAATGAAGTTT+TGG | - | chr3.4:80750565-80750584 | MS.gene84712:CDS | 30.0% |
| AAGAAACTGAAAGAGGTGAT+AGG | - | chr3.4:80749924-80749943 | MS.gene84712:CDS | 35.0% | |
| AGAGAATTGAAGGAAAAGGT+TGG | - | chr3.4:80750746-80750765 | MS.gene84712:CDS | 35.0% | |
| AGAGGTGATAGGATTAATGA+AGG | - | chr3.4:80749935-80749954 | MS.gene84712:CDS | 35.0% | |
| AGTTAAAGAATTTGGGGAGA+GGG | - | chr3.4:80750592-80750611 | MS.gene84712:CDS | 35.0% | |
| CAAGGTGATTGTTGAGATAA+TGG | - | chr3.4:80750520-80750539 | MS.gene84712:CDS | 35.0% | |
| GAACGGCGATTAAACATTTA+AGG | + | chr3.4:80750169-80750188 | None:intergenic | 35.0% | |
| GAGAATTGAAGGAAAAGGTT+GGG | - | chr3.4:80750747-80750766 | MS.gene84712:CDS | 35.0% | |
| ! | AAACTTGGTTGTCTTGGTTT+TGG | - | chr3.4:80750617-80750636 | MS.gene84712:CDS | 35.0% |
| ! | AGAGAGAATGATGATGATGA+TGG | - | chr3.4:80750688-80750707 | MS.gene84712:CDS | 35.0% |
| ! | TGTTCACTCATTGGAGTATT+AGG | + | chr3.4:80750497-80750516 | None:intergenic | 35.0% |
| !! | TGTTGAGATAATGGATTTGG+TGG | - | chr3.4:80750529-80750548 | MS.gene84712:CDS | 35.0% |
| !! | TTAGGTCTGTTGATTCCATT+AGG | - | chr3.4:80750861-80750880 | MS.gene84712:CDS | 35.0% |
| !!! | GGTTTGTGTTTTGAAGAGAT+TGG | - | chr3.4:80750655-80750674 | MS.gene84712:CDS | 35.0% |
| !!! | TTTTGTCTCTGAAGTTTGAG+AGG | + | chr3.4:80750274-80750293 | None:intergenic | 35.0% |
| AATTGAAGGAAAAGGTTGGG+AGG | - | chr3.4:80750750-80750769 | MS.gene84712:CDS | 40.0% | |
| ACCTTGTTCTGTTCACTCAT+TGG | + | chr3.4:80750506-80750525 | None:intergenic | 40.0% | |
| AGAACTCTTGGTTTCTTCCT+TGG | - | chr3.4:80750371-80750390 | MS.gene84712:CDS | 40.0% | |
| AGTTTCTACCACCAGTGTAA+GGG | + | chr3.4:80750244-80750263 | None:intergenic | 40.0% | |
| ATCTTGGGAACTCTCTTCTT+GGG | - | chr3.4:80750304-80750323 | MS.gene84712:CDS | 40.0% | |
| CCATTAGGTTTCCTTCTTCA+AGG | - | chr3.4:80750876-80750895 | MS.gene84712:CDS | 40.0% | |
| CCTTGAAGAAGGAAACCTAA+TGG | + | chr3.4:80750879-80750898 | None:intergenic | 40.0% | |
| GAGTTAAAGAATTTGGGGAG+AGG | - | chr3.4:80750591-80750610 | MS.gene84712:CDS | 40.0% | |
| GGTGAGAGAATTGAAGGAAA+AGG | - | chr3.4:80750742-80750761 | MS.gene84712:CDS | 40.0% | |
| GGTTGAGGAGAAGAAATTCT+GGG | - | chr3.4:80750715-80750734 | MS.gene84712:CDS | 40.0% | |
| GTTCACCACATCATCAAACA+CGG | - | chr3.4:80750185-80750204 | MS.gene84712:CDS | 40.0% | |
| GTTGAAAGAGTCATGTGTTG+TGG | + | chr3.4:80750024-80750043 | None:intergenic | 40.0% | |
| TAGTTTCTACCACCAGTGTA+AGG | + | chr3.4:80750245-80750264 | None:intergenic | 40.0% | |
| TATCTTGGGAACTCTCTTCT+TGG | - | chr3.4:80750303-80750322 | MS.gene84712:CDS | 40.0% | |
| TCCAATGAGTGAACAGAACA+AGG | - | chr3.4:80750502-80750521 | MS.gene84712:CDS | 40.0% | |
| TGGTTGAGGAGAAGAAATTC+TGG | - | chr3.4:80750714-80750733 | MS.gene84712:CDS | 40.0% | |
| TTTCCCAATTCCTTCCATCA+AGG | + | chr3.4:80750471-80750490 | None:intergenic | 40.0% | |
| ! | ATGAAGGTGTATAGGGAAGA+AGG | - | chr3.4:80750773-80750792 | MS.gene84712:CDS | 40.0% |
| ! | GGTTTGATGACAGAGTTCTT+AGG | - | chr3.4:80750843-80750862 | MS.gene84712:CDS | 40.0% |
| ! | TGAAGGTGTATAGGGAAGAA+GGG | - | chr3.4:80750774-80750793 | MS.gene84712:CDS | 40.0% |
| !! | AATGATGATGATGATGGAGG+TGG | - | chr3.4:80750694-80750713 | MS.gene84712:CDS | 40.0% |
| !! | ATGGATTTGGTGGAAGATGA+TGG | - | chr3.4:80750539-80750558 | MS.gene84712:CDS | 40.0% |
| !! | GAGAATGATGATGATGATGG+AGG | - | chr3.4:80750691-80750710 | MS.gene84712:CDS | 40.0% |
| !! | GGAGAAGAAATTCTGGGATT+TGG | - | chr3.4:80750721-80750740 | MS.gene84712:CDS | 40.0% |
| !! | GGATTTGGTGAGAGAATTGA+AGG | - | chr3.4:80750736-80750755 | MS.gene84712:CDS | 40.0% |
| !! | GTGTTTGATGATGTGGTGAA+CGG | + | chr3.4:80750186-80750205 | None:intergenic | 40.0% |
| !! | TGATTCTTTGTTGGCCTTGA+TGG | - | chr3.4:80750454-80750473 | MS.gene84712:CDS | 40.0% |
| !! | TGGATTTGGTGGAAGATGAT+GGG | - | chr3.4:80750540-80750559 | MS.gene84712:CDS | 40.0% |
| !! | TTTTGGAGAAGTGGTGGAAT+TGG | - | chr3.4:80750634-80750653 | MS.gene84712:CDS | 40.0% |
| !!! | AAGAGGTGTTTGTGTTTTGG+AGG | + | chr3.4:80750047-80750066 | None:intergenic | 40.0% |
| !!! | AGAGGTGTTTGTGTTTTGGA+GGG | + | chr3.4:80750046-80750065 | None:intergenic | 40.0% |
| !!! | GTCAAGAGGTGTTTGTGTTT+TGG | + | chr3.4:80750050-80750069 | None:intergenic | 40.0% |
| !!! | TTGTCTTGGTTTTGGAGAAG+TGG | - | chr3.4:80750625-80750644 | MS.gene84712:CDS | 40.0% |
| AACTCTCTTCTTGGGTTCGA+TGG | - | chr3.4:80750312-80750331 | MS.gene84712:CDS | 45.0% | |
| ACAGAAGAACCGAGTCTGAT+CGG | - | chr3.4:80750822-80750841 | MS.gene84712:CDS | 45.0% | |
| ACCACCAGTGTAAGGGTAAA+CGG | + | chr3.4:80750237-80750256 | None:intergenic | 45.0% | |
| AGGGAGAAACTTGGTTGTCT+TGG | - | chr3.4:80750611-80750630 | MS.gene84712:CDS | 45.0% | |
| CAGAGGAAAGAAGAGTCAAG+AGG | + | chr3.4:80750064-80750083 | None:intergenic | 45.0% | |
| CTCTTGACTCTTCTTTCCTC+TGG | - | chr3.4:80750062-80750081 | MS.gene84712:CDS | 45.0% | |
| GTCATCAAACCGATCAGACT+CGG | + | chr3.4:80750834-80750853 | None:intergenic | 45.0% | |
| ! | ACTCTTCTTTCCTCTGGAGA+CGG | - | chr3.4:80750068-80750087 | MS.gene84712:CDS | 45.0% |
| ! | AGTTGTGAGTGGTTTGGAGA+CGG | + | chr3.4:80750136-80750155 | None:intergenic | 45.0% |
| ! | TGGCCTTGATGGAAGGAATT+GGG | - | chr3.4:80750465-80750484 | MS.gene84712:CDS | 45.0% |
| ! | TGGGAGGATGAAGGTGTATA+GGG | - | chr3.4:80750766-80750785 | MS.gene84712:CDS | 45.0% |
| ! | TTGGGAGGATGAAGGTGTAT+AGG | - | chr3.4:80750765-80750784 | MS.gene84712:CDS | 45.0% |
| !! | AGGTACCGTGTTTGATGATG+TGG | + | chr3.4:80750193-80750212 | None:intergenic | 45.0% |
| !! | CTCTGAAGTTTGAGAGGTTG+AGG | + | chr3.4:80750268-80750287 | None:intergenic | 45.0% |
| !! | CTGTGTGCTTCTAGAACTCT+TGG | - | chr3.4:80750359-80750378 | MS.gene84712:CDS | 45.0% |
| !! | GAGTTCTAGAAGCACACAGA+AGG | + | chr3.4:80750358-80750377 | None:intergenic | 45.0% |
| !! | GGATTTGGTGGAAGATGATG+GGG | - | chr3.4:80750541-80750560 | MS.gene84712:CDS | 45.0% |
| !! | TCTTTGTTGGCCTTGATGGA+AGG | - | chr3.4:80750458-80750477 | MS.gene84712:CDS | 45.0% |
| !! | TTGGCCTTGATGGAAGGAAT+TGG | - | chr3.4:80750464-80750483 | MS.gene84712:CDS | 45.0% |
| !!! | TCTTGGTTTTGGAGAAGTGG+TGG | - | chr3.4:80750628-80750647 | MS.gene84712:CDS | 45.0% |
| AGAAGGGAGGCTTAGCAGTA+CGG | - | chr3.4:80750790-80750809 | MS.gene84712:CDS | 50.0% | |
| AGGTGTATAGGGAAGAAGGG+AGG | - | chr3.4:80750777-80750796 | MS.gene84712:CDS | 50.0% | |
| AGTCATGTGTTGTGGCACGT+AGG | + | chr3.4:80750016-80750035 | None:intergenic | 50.0% | |
| CTCTCCGTTTACCCTTACAC+TGG | - | chr3.4:80750230-80750249 | MS.gene84712:CDS | 50.0% | |
| CTGCTGAGTTGTGAGTGGTT+TGG | + | chr3.4:80750142-80750161 | None:intergenic | 50.0% | |
| GGAAAAGGTTGGGAGGATGA+AGG | - | chr3.4:80750757-80750776 | MS.gene84712:CDS | 50.0% | |
| GGTAAACGGAGAGCTGATCA+CGG | + | chr3.4:80750223-80750242 | None:intergenic | 50.0% | |
| GGTGAAACGACGTCAGAGAA+AGG | - | chr3.4:80750392-80750411 | MS.gene84712:CDS | 50.0% | |
| TCCGTTTACCCTTACACTGG+TGG | - | chr3.4:80750233-80750252 | MS.gene84712:CDS | 50.0% | |
| TCTCTGACGTCGTTTCACCA+AGG | + | chr3.4:80750391-80750410 | None:intergenic | 50.0% | |
| TTCTTGGGTTCGATGGTACG+CGG | - | chr3.4:80750319-80750338 | MS.gene84712:CDS | 50.0% | |
| TTTGGGGAGAGGGAGAAACT+TGG | - | chr3.4:80750602-80750621 | MS.gene84712:CDS | 50.0% | |
| !! | GATGATGATGGAGGTGGTTG+AGG | - | chr3.4:80750700-80750719 | MS.gene84712:CDS | 50.0% |
| !! | GGTGGAAGATGATGGGGTTA+TGG | - | chr3.4:80750547-80750566 | MS.gene84712:CDS | 50.0% |
| AAACGGAGAGCTGATCACGG+AGG | + | chr3.4:80750220-80750239 | None:intergenic | 55.0% | |
| CATGTGTTGTGGCACGTAGG+AGG | + | chr3.4:80750013-80750032 | None:intergenic | 55.0% | |
| GAGCTGATCACGGAGGATGA+AGG | + | chr3.4:80750213-80750232 | None:intergenic | 55.0% | |
| GGCTACTGCTGAGTTGTGAG+TGG | + | chr3.4:80750147-80750166 | None:intergenic | 55.0% | |
| AAGCTCCACCGCAGAGGATG+CGG | + | chr3.4:80750105-80750124 | None:intergenic | 60.0% | |
| CATGAGAAGCTCCACCGCAG+AGG | + | chr3.4:80750111-80750130 | None:intergenic | 60.0% | |
| CTCTGCGGTGGAGCTTCTCA+TGG | - | chr3.4:80750109-80750128 | MS.gene84712:CDS | 60.0% | |
| ACGTGCCACCGCATCCTCTG+CGG | - | chr3.4:80750094-80750113 | MS.gene84712:CDS | 65.0% | |
| GGCACGTGAACCGTCTCCAG+AGG | + | chr3.4:80750081-80750100 | None:intergenic | 65.0% | |
| CTCCACCGCAGAGGATGCGG+TGG | + | chr3.4:80750102-80750121 | None:intergenic | 70.0% | |
| TGCCACCGCATCCTCTGCGG+TGG | - | chr3.4:80750097-80750116 | MS.gene84712:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 80749921 | 80750907 | 80749921 | ID=MS.gene84712 |
| chr3.4 | mRNA | 80749921 | 80750907 | 80749921 | ID=MS.gene84712.t1;Parent=MS.gene84712 |
| chr3.4 | exon | 80749921 | 80750907 | 80749921 | ID=MS.gene84712.t1.exon1;Parent=MS.gene84712.t1 |
| chr3.4 | CDS | 80749921 | 80750907 | 80749921 | ID=cds.MS.gene84712.t1;Parent=MS.gene84712.t1 |
| Gene Sequence |
| Protein sequence |
