Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89369.t1 | XP_003621026.1 | 99.3 | 139 | 1 | 0 | 1 | 139 | 101 | 239 | 1.30E-57 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89369.t1 | Q67YI9 | 68.8 | 96 | 22 | 2 | 1 | 96 | 101 | 188 | 1.9e-28 | 127.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89369.t1 | A2Q4X4 | 99.3 | 139 | 1 | 0 | 1 | 139 | 101 | 239 | 9.6e-58 | 232.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene89369 | 0.815176 | 1.06E-51 | -1.69E-46 |
| MS.gene049193 | MS.gene89369 | 0.814496 | 1.50E-51 | -1.69E-46 |
| MS.gene049283 | MS.gene89369 | 0.817255 | 3.63E-52 | -1.69E-46 |
| MS.gene049368 | MS.gene89369 | 0.815892 | 7.32E-52 | -1.69E-46 |
| MS.gene049807 | MS.gene89369 | 0.826492 | 2.63E-54 | -1.69E-46 |
| MS.gene049983 | MS.gene89369 | 0.831553 | 1.56E-55 | -1.69E-46 |
| MS.gene050084 | MS.gene89369 | 0.816898 | 4.36E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene89369 | 0.829307 | 5.54E-55 | -1.69E-46 |
| MS.gene050213 | MS.gene89369 | 0.828558 | 8.41E-55 | -1.69E-46 |
| MS.gene050224 | MS.gene89369 | 0.831577 | 1.54E-55 | -1.69E-46 |
| MS.gene050359 | MS.gene89369 | 0.874442 | 6.73E-68 | -1.69E-46 |
| MS.gene050361 | MS.gene89369 | 0.803124 | 4.05E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene89369 | 0.864697 | 1.01E-64 | -1.69E-46 |
| MS.gene050551 | MS.gene89369 | 0.837539 | 4.90E-57 | -1.69E-46 |
| MS.gene050646 | MS.gene89369 | 0.888318 | 6.57E-73 | -1.69E-46 |
| MS.gene050654 | MS.gene89369 | 0.872295 | 3.55E-67 | -1.69E-46 |
| MS.gene050689 | MS.gene89369 | 0.806863 | 6.69E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene89369 | 0.823452 | 1.37E-53 | -1.69E-46 |
| MS.gene050931 | MS.gene89369 | 0.816415 | 5.60E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene89369 | 0.803697 | 3.08E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene89369 | 0.803459 | 3.45E-49 | -1.69E-46 |
| MS.gene051043 | MS.gene89369 | 0.824547 | 7.61E-54 | -1.69E-46 |
| MS.gene051102 | MS.gene89369 | 0.801165 | 1.02E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene89369 | 0.836474 | 9.16E-57 | -1.69E-46 |
| MS.gene051343 | MS.gene89369 | 0.837021 | 6.65E-57 | -1.69E-46 |
| MS.gene051381 | MS.gene89369 | 0.847274 | 1.28E-59 | -1.69E-46 |
| MS.gene051382 | MS.gene89369 | 0.809831 | 1.56E-50 | -1.69E-46 |
| MS.gene051642 | MS.gene89369 | 0.808972 | 2.38E-50 | -1.69E-46 |
| MS.gene051852 | MS.gene89369 | 0.802622 | 5.14E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene89369 | 0.85128 | 9.83E-61 | -1.69E-46 |
| MS.gene052242 | MS.gene89369 | -0.820538 | 6.51E-53 | -1.69E-46 |
| MS.gene052307 | MS.gene89369 | 0.873516 | 1.38E-67 | -1.69E-46 |
| MS.gene052335 | MS.gene89369 | 0.813513 | 2.46E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene89369 | 0.807565 | 4.75E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene89369 | 0.823936 | 1.06E-53 | -1.69E-46 |
| MS.gene052514 | MS.gene89369 | 0.819436 | 1.16E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene89369 | 0.80013 | 1.66E-48 | -1.69E-46 |
| MS.gene052609 | MS.gene89369 | 0.893464 | 6.13E-75 | -1.69E-46 |
| MS.gene052776 | MS.gene89369 | 0.828535 | 8.52E-55 | -1.69E-46 |
| MS.gene052932 | MS.gene89369 | 0.81307 | 3.08E-51 | -1.69E-46 |
| MS.gene052994 | MS.gene89369 | 0.808311 | 3.30E-50 | -1.69E-46 |
| MS.gene053058 | MS.gene89369 | 0.812927 | 3.31E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene89369 | 0.826814 | 2.21E-54 | -1.69E-46 |
| MS.gene053143 | MS.gene89369 | 0.884209 | 2.33E-71 | -1.69E-46 |
| MS.gene053486 | MS.gene89369 | 0.808998 | 2.35E-50 | -1.69E-46 |
| MS.gene05350 | MS.gene89369 | 0.807713 | 4.42E-50 | -1.69E-46 |
| MS.gene053618 | MS.gene89369 | 0.810433 | 1.16E-50 | -1.69E-46 |
| MS.gene053620 | MS.gene89369 | 0.856068 | 4.13E-62 | -1.69E-46 |
| MS.gene054178 | MS.gene89369 | 0.832041 | 1.19E-55 | -1.69E-46 |
| MS.gene054585 | MS.gene89369 | 0.821425 | 4.07E-53 | -1.69E-46 |
| MS.gene054824 | MS.gene89369 | 0.833782 | 4.38E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene89369 | 0.838504 | 2.77E-57 | -1.69E-46 |
| MS.gene054893 | MS.gene89369 | 0.841874 | 3.65E-58 | -1.69E-46 |
| MS.gene055469 | MS.gene89369 | 0.822933 | 1.82E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene89369 | 0.853495 | 2.30E-61 | -1.69E-46 |
| MS.gene055864 | MS.gene89369 | 0.812264 | 4.63E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene89369 | 0.834907 | 2.28E-56 | -1.69E-46 |
| MS.gene056097 | MS.gene89369 | 0.851774 | 7.13E-61 | -1.69E-46 |
| MS.gene05615 | MS.gene89369 | 0.806272 | 8.92E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene89369 | 0.833292 | 5.80E-56 | -1.69E-46 |
| MS.gene056308 | MS.gene89369 | 0.843352 | 1.48E-58 | -1.69E-46 |
| MS.gene056507 | MS.gene89369 | 0.846124 | 2.64E-59 | -1.69E-46 |
| MS.gene056578 | MS.gene89369 | 0.814049 | 1.88E-51 | -1.69E-46 |
| MS.gene056677 | MS.gene89369 | 0.8169 | 4.36E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene89369 | 0.810621 | 1.05E-50 | -1.69E-46 |
| MS.gene056831 | MS.gene89369 | 0.811454 | 6.95E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene89369 | 0.806733 | 7.13E-50 | -1.69E-46 |
| MS.gene057174 | MS.gene89369 | 0.816634 | 5.00E-52 | -1.69E-46 |
| MS.gene057919 | MS.gene89369 | 0.837545 | 4.88E-57 | -1.69E-46 |
| MS.gene058010 | MS.gene89369 | 0.801608 | 8.31E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene89369 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene058292 | MS.gene89369 | 0.817543 | 3.12E-52 | -1.69E-46 |
| MS.gene058330 | MS.gene89369 | 0.810489 | 1.12E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene89369 | 0.818111 | 2.32E-52 | -1.69E-46 |
| MS.gene058638 | MS.gene89369 | 0.826409 | 2.76E-54 | -1.69E-46 |
| MS.gene058639 | MS.gene89369 | 0.821975 | 3.03E-53 | -1.69E-46 |
| MS.gene058647 | MS.gene89369 | 0.802771 | 4.79E-49 | -1.69E-46 |
| MS.gene058649 | MS.gene89369 | 0.828841 | 7.18E-55 | -1.69E-46 |
| MS.gene058758 | MS.gene89369 | 0.823931 | 1.06E-53 | -1.69E-46 |
| MS.gene059007 | MS.gene89369 | 0.865431 | 5.93E-65 | -1.69E-46 |
| MS.gene059170 | MS.gene89369 | 0.839506 | 1.52E-57 | -1.69E-46 |
| MS.gene059439 | MS.gene89369 | 0.810718 | 1.00E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene89369 | 0.810144 | 1.33E-50 | -1.69E-46 |
| MS.gene059965 | MS.gene89369 | 0.826765 | 2.27E-54 | -1.69E-46 |
| MS.gene060259 | MS.gene89369 | 0.815361 | 9.62E-52 | -1.69E-46 |
| MS.gene060343 | MS.gene89369 | -0.808463 | 3.06E-50 | -1.69E-46 |
| MS.gene060563 | MS.gene89369 | 0.822542 | 2.24E-53 | -1.69E-46 |
| MS.gene060777 | MS.gene89369 | 0.802502 | 5.44E-49 | -1.69E-46 |
| MS.gene060785 | MS.gene89369 | 0.801283 | 9.68E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene89369 | 0.848656 | 5.33E-60 | -1.69E-46 |
| MS.gene060933 | MS.gene89369 | 0.844725 | 6.33E-59 | -1.69E-46 |
| MS.gene060937 | MS.gene89369 | 0.817327 | 3.49E-52 | -1.69E-46 |
| MS.gene061006 | MS.gene89369 | 0.862961 | 3.50E-64 | -1.69E-46 |
| MS.gene061100 | MS.gene89369 | 0.8833 | 5.04E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAATCTAAAATGATTTAA+AGG | + | 50655:12160-12179 | MS.gene89369:intron | 10.0% |
| !!! | GGTTACATTATAATATTTTA+CGG | - | 50655:11816-11835 | MS.gene89369:intergenic | 15.0% |
| !! | ACTAACAGCATATAAATATT+AGG | - | 50655:11757-11776 | MS.gene89369:intergenic | 20.0% |
| !! | AGAACATTCTTATCAAATAT+CGG | + | 50655:11707-11726 | MS.gene89369:CDS | 20.0% |
| !!! | AGGAATATTACATTTTTATC+AGG | - | 50655:11837-11856 | MS.gene89369:intergenic | 20.0% |
| !!! | ATTTTCTTAAACAATTTTCC+AGG | + | 50655:12361-12380 | MS.gene89369:intron | 20.0% |
| !!! | TGATTTTAGATTGAGTATAA+TGG | - | 50655:12328-12347 | MS.gene89369:intergenic | 20.0% |
| ! | ACTACAATTATGATGATACA+AGG | + | 50655:12646-12665 | MS.gene89369:CDS | 25.0% |
| ! | ATTATGGATTTACCAAAACT+GGG | - | 50655:12118-12137 | MS.gene89369:intergenic | 25.0% |
| ! | GGAATCAATATATTGAAAGT+CGG | - | 50655:11875-11894 | MS.gene89369:intergenic | 25.0% |
| ! | TTCTTTCTTTATCATTCACA+AGG | - | 50655:11951-11970 | MS.gene89369:intergenic | 25.0% |
| ! | TTTATAATCCAGAAATAGAG+AGG | - | 50655:12145-12164 | MS.gene89369:intergenic | 25.0% |
| !! | GCAAAATAAAGGATTTTCAT+TGG | - | 50655:12216-12235 | MS.gene89369:intergenic | 25.0% |
| !! | TTCTAGTTATATAAAGCATC+AGG | + | 50655:12058-12077 | MS.gene89369:intron | 25.0% |
| !!! | TTTCAATATATTGATTCCAG+TGG | + | 50655:11877-11896 | MS.gene89369:CDS | 25.0% |
| ATATCAGTAGAATTGTTGAG+AGG | - | 50655:12022-12041 | MS.gene89369:intergenic | 30.0% | |
| GATTATGGATTTACCAAAAC+TGG | - | 50655:12119-12138 | MS.gene89369:intergenic | 30.0% | |
| GTATGACATGGAAAAATTGT+GGG | + | 50655:12263-12282 | MS.gene89369:intron | 30.0% | |
| TATGGAAGAGAAAGAGAAAT+GGG | + | 50655:12507-12526 | MS.gene89369:CDS | 30.0% | |
| TGATAAAGAAAGAATCGTAG+AGG | + | 50655:11957-11976 | MS.gene89369:CDS | 30.0% | |
| TTATGGAAGAGAAAGAGAAA+TGG | + | 50655:12506-12525 | MS.gene89369:CDS | 30.0% | |
| TTATGGATTTACCAAAACTG+GGG | - | 50655:12117-12136 | MS.gene89369:intergenic | 30.0% | |
| !!! | TTTTTAATTTCCCCCAGTTT+TGG | + | 50655:12103-12122 | MS.gene89369:intron | 30.0% |
| AAGAAAGAATCGTAGAGGTT+AGG | + | 50655:11962-11981 | MS.gene89369:CDS | 35.0% | |
| AGAGATTATGAGGAACGTTA+CGG | + | 50655:12576-12595 | MS.gene89369:CDS | 35.0% | |
| ATTATGATGATACAAGGAGC+AGG | + | 50655:12652-12671 | MS.gene89369:CDS | 35.0% | |
| GAAATAGAGAGGACAGATTA+TGG | - | 50655:12134-12153 | MS.gene89369:intergenic | 35.0% | |
| GATAGAAATGGCTATGGTTA+TGG | + | 50655:12489-12508 | MS.gene89369:CDS | 35.0% | |
| GGTATGACATGGAAAAATTG+TGG | + | 50655:12262-12281 | MS.gene89369:intron | 35.0% | |
| TAATCTGTCCTCTCTATTTC+TGG | + | 50655:12134-12153 | MS.gene89369:intron | 35.0% | |
| TATGGATTTACCAAAACTGG+GGG | - | 50655:12116-12135 | MS.gene89369:intergenic | 35.0% | |
| TCGTTATGGTAGAGATTATG+AGG | + | 50655:12566-12585 | MS.gene89369:CDS | 35.0% | |
| ACGGTCATTTGATCGAAACT+AGG | - | 50655:11797-11816 | MS.gene89369:intergenic | 40.0% | |
| ACGGTTACAGAGATGATGAT+AGG | + | 50655:12604-12623 | MS.gene89369:CDS | 40.0% | |
| ATACCACCAGTTACAAGAGT+TGG | - | 50655:12247-12266 | MS.gene89369:intergenic | 40.0% | |
| ATTATGAGGAACGTTACGGT+AGG | + | 50655:12580-12599 | MS.gene89369:CDS | 40.0% | |
| CGGTTACAGAGATGATGATA+GGG | + | 50655:12605-12624 | MS.gene89369:CDS | 40.0% | |
| CTGTTAGTCAATTTCCACAC+AGG | + | 50655:11769-11788 | MS.gene89369:intron | 40.0% | |
| GAGATCAAGGAAACAATGTC+AGG | + | 50655:11902-11921 | MS.gene89369:CDS | 40.0% | |
| GAGGAAGATAGAAATGGCTA+TGG | + | 50655:12483-12502 | MS.gene89369:CDS | 40.0% | |
| GGTTACAGAGATGATGATAG+GGG | + | 50655:12606-12625 | MS.gene89369:CDS | 40.0% | |
| TACCACCAGTTACAAGAGTT+GGG | - | 50655:12246-12265 | MS.gene89369:intergenic | 40.0% | |
| TTATGAGGAACGTTACGGTA+GGG | + | 50655:12581-12600 | MS.gene89369:CDS | 40.0% | |
| TTGTAACTGGTGGTATGACA+TGG | + | 50655:12251-12270 | MS.gene89369:intron | 40.0% | |
| ! | TGGGTGTTGTGGCAAAATAA+AGG | - | 50655:12227-12246 | MS.gene89369:intergenic | 40.0% |
| AAGAAGATCGTTATGCGAGC+AGG | + | 50655:12460-12479 | MS.gene89369:CDS | 45.0% | |
| AATCGCGATGGAGATCGTTA+TGG | + | 50655:12552-12571 | MS.gene89369:CDS | 45.0% | |
| AATCGTAGAGGTTAGGCAGA+AGG | + | 50655:11969-11988 | MS.gene89369:CDS | 45.0% | |
| AGAAGATCGTTATGCGAGCA+GGG | + | 50655:12461-12480 | MS.gene89369:CDS | 45.0% | |
| AGCAGGGAGGAAGATAGAAA+TGG | + | 50655:12477-12496 | MS.gene89369:CDS | 45.0% | |
| AGGTTTCGCAATAACACACC+AGG | + | 50655:12381-12400 | MS.gene89369:intron | 45.0% | |
| ATCGAAACTAGGTACCTGTG+TGG | - | 50655:11786-11805 | MS.gene89369:intergenic | 45.0% | |
| CAACACCCAACTCTTGTAAC+TGG | + | 50655:12238-12257 | MS.gene89369:intron | 45.0% | |
| GATCGTGATTTCGATGACGA+TGG | + | 50655:12687-12706 | MS.gene89369:CDS | 45.0% | |
| GATTCCAGTGGAAGAGATCA+AGG | + | 50655:11889-11908 | MS.gene89369:CDS | 45.0% | |
| GCAGAAGGCTGCTGTTAATA+GGG | + | 50655:11984-12003 | MS.gene89369:CDS | 45.0% | |
| GGAAAAATTGTGGGTCTCGT+TGG | + | 50655:12272-12291 | MS.gene89369:intron | 45.0% | |
| GTCGGACAAAGTCTGTTCAT+AGG | - | 50655:11857-11876 | MS.gene89369:intergenic | 45.0% | |
| GTTTCCTTGATCTCTTCCAC+TGG | - | 50655:11896-11915 | MS.gene89369:intergenic | 45.0% | |
| TTTCGCAATAACACACCAGG+TGG | + | 50655:12384-12403 | MS.gene89369:CDS | 45.0% | |
| ! | GTTACAAGAGTTGGGTGTTG+TGG | - | 50655:12238-12257 | MS.gene89369:intergenic | 45.0% |
| ! | TGGTGTGTTATTGCGAAACC+TGG | - | 50655:12382-12401 | MS.gene89369:intergenic | 45.0% |
| !! | CACTCAAGCATTGGAAGCTA+TGG | + | 50655:12426-12445 | MS.gene89369:CDS | 45.0% |
| !! | TGGGCTCTAGAGATGATGAT+CGG | + | 50655:12526-12545 | MS.gene89369:CDS | 45.0% |
| CACCCAACTCTTGTAACTGG+TGG | + | 50655:12241-12260 | MS.gene89369:intron | 50.0% | |
| CCAATGCTTGAGTGTGAACC+AGG | - | 50655:12420-12439 | MS.gene89369:intergenic | 50.0% | |
| CCAGGTCTATACATTCCACC+TGG | - | 50655:12402-12421 | MS.gene89369:intergenic | 50.0% | |
| CCAGGTGGAATGTATAGACC+TGG | + | 50655:12399-12418 | MS.gene89369:CDS | 50.0% | |
| CCTGGTTCACACTCAAGCAT+TGG | + | 50655:12417-12436 | MS.gene89369:CDS | 50.0% | |
| GATGATCGGTACAATCGCGA+TGG | + | 50655:12540-12559 | MS.gene89369:CDS | 50.0% | |
| GGCAGAAGGCTGCTGTTAAT+AGG | + | 50655:11983-12002 | MS.gene89369:CDS | 50.0% | |
| AGATCGTTATGCGAGCAGGG+AGG | + | 50655:12464-12483 | MS.gene89369:CDS | 55.0% | |
| GAGGAACGTTACGGTAGGGA+CGG | + | 50655:12585-12604 | MS.gene89369:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50655 | gene | 11697 | 12724 | 11697 | ID=MS.gene89369 |
| 50655 | mRNA | 11697 | 12724 | 11697 | ID=MS.gene89369.t1;Parent=MS.gene89369 |
| 50655 | exon | 11697 | 11728 | 11697 | ID=MS.gene89369.t1.exon1;Parent=MS.gene89369.t1 |
| 50655 | CDS | 11697 | 11728 | 11697 | ID=cds.MS.gene89369.t1;Parent=MS.gene89369.t1 |
| 50655 | exon | 11865 | 12010 | 11865 | ID=MS.gene89369.t1.exon2;Parent=MS.gene89369.t1 |
| 50655 | CDS | 11865 | 12010 | 11865 | ID=cds.MS.gene89369.t1;Parent=MS.gene89369.t1 |
| 50655 | exon | 12383 | 12724 | 12383 | ID=MS.gene89369.t1.exon3;Parent=MS.gene89369.t1 |
| 50655 | CDS | 12383 | 12724 | 12383 | ID=cds.MS.gene89369.t1;Parent=MS.gene89369.t1 |
| Gene Sequence |
| Protein sequence |
