AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92668


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92668.t1 MTR_3g033420 89.513 267 21 1 1 260 137 403 3.97e-167 468
MS.gene92668.t1 MTR_3g033150 92.857 154 11 0 107 260 1 154 4.13e-93 271
MS.gene92668.t1 MTR_7g101840 37.247 247 155 0 13 259 307 553 1.38e-47 166
MS.gene92668.t1 MTR_3g118290 35.865 237 152 0 24 260 335 571 4.22e-45 159
MS.gene92668.t1 MTR_1g063850 34.034 238 157 0 22 259 320 557 4.31e-40 145
MS.gene92668.t1 MTR_0172s0030 39.076 238 137 4 17 248 255 490 1.96e-39 143
MS.gene92668.t1 MTR_1g007370 32.258 248 161 2 13 259 306 547 8.52e-36 134
MS.gene92668.t1 MTR_3g090900 27.519 258 180 4 7 260 260 514 1.12e-16 79.7
MS.gene92668.t1 MTR_1g007370 38.202 89 55 0 13 101 306 394 1.91e-15 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92668.t1 AT5G13730 58.300 247 103 0 12 258 168 414 2.25e-100 299
MS.gene92668.t1 AT2G36990 35.146 239 155 0 21 259 305 543 1.92e-45 160
MS.gene92668.t1 AT1G08540 35.021 237 154 0 24 260 333 569 2.12e-43 155
MS.gene92668.t1 AT1G64860 38.776 245 136 5 13 248 249 488 1.38e-39 144
MS.gene92668.t1 AT3G53920 31.933 238 161 1 23 259 332 569 4.18e-34 129
MS.gene92668.t1 AT1G64860 38.117 223 124 5 13 226 249 466 8.00e-33 125
MS.gene92668.t1 AT5G24120 25.688 218 154 3 46 260 305 517 5.93e-15 74.7
MS.gene92668.t1 AT5G24120 25.688 218 154 3 46 260 305 517 5.93e-15 74.7
MS.gene92668.t1 AT5G24120 25.688 218 154 3 46 260 305 517 5.93e-15 74.7
MS.gene92668.t1 AT3G53920 40.506 79 47 0 23 101 332 410 2.00e-14 72.8

Find 46 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTCTGTCCAATGAAAT+TGG 0.295581 3.1:+23814038 None:intergenic
ACAAGGCATGATTAAAGCTT+TGG 0.334814 3.1:-23814828 MS.gene92668:CDS
TATTTATAGGGAGAAAAGTA+TGG 0.343959 3.1:-23814197 MS.gene92668:intron
GGAACCATTGGGCTTCTTCA+TGG 0.361844 3.1:-23814914 MS.gene92668:CDS
ATATTATACTTTGGACTCAA+TGG 0.405310 3.1:-23813751 MS.gene92668:CDS
TTCTCCGGAATCATTTCAAC+TGG 0.414074 3.1:+23813853 None:intergenic
GAGCAGACAACCAATTTCAT+TGG 0.418328 3.1:-23814048 MS.gene92668:CDS
AAGCATTCAAGATCTCATTC+AGG 0.419494 3.1:-23815013 MS.gene92668:intron
AGCTGTCTACATATGTTTAC+TGG 0.421708 3.1:-23814859 MS.gene92668:CDS
GGAGAAAATGTTTGAGAGAC+AGG 0.428727 3.1:-23813836 MS.gene92668:CDS
CACCGGTAATCTGACTAATC+TGG 0.431896 3.1:+23814796 None:intergenic
GAGATTGTAATATTATACTT+TGG 0.445378 3.1:-23813760 MS.gene92668:CDS
GTCCAGATTAGTCAGATTAC+CGG 0.449365 3.1:-23814798 MS.gene92668:intron
GGGACCAGTTGAAATGATTC+CGG 0.449561 3.1:-23813857 MS.gene92668:CDS
CTATCTCCTCAAAAGACAAA+GGG 0.452640 3.1:+23813722 None:intergenic
GAGAAATTTGATCCGGATAG+AGG 0.468549 3.1:-23814887 MS.gene92668:CDS
GAAAGCTGTTGAAACTATCA+AGG 0.471546 3.1:-23813699 MS.gene92668:CDS
GAGAGACAGGTAATGAAGGA+AGG 0.490016 3.1:-23813823 MS.gene92668:CDS
GTTTGAGAGACAGGTAATGA+AGG 0.499603 3.1:-23813827 MS.gene92668:CDS
AGAGACAGGTAATGAAGGAA+GGG 0.501313 3.1:-23813822 MS.gene92668:CDS
TTTAACCAGGAAGGAACCAT+TGG 0.506733 3.1:-23814926 MS.gene92668:intron
TCAATCGCAACACGTTATCA+AGG 0.511860 3.1:-23815045 MS.gene92668:CDS
GAGAGGATCAGACAGATTCA+TGG 0.512718 3.1:-23813676 MS.gene92668:CDS
GACAGCTTACATCCTCTATC+CGG 0.518858 3.1:+23814875 None:intergenic
TGGAGCAGAGAAATTTGATC+CGG 0.520546 3.1:-23814894 MS.gene92668:CDS
GCAACACGTTATCAAGGAAA+AGG 0.522492 3.1:-23815039 MS.gene92668:CDS
TATAATGCAGGAGATTATAC+CGG 0.529279 3.1:-23813878 MS.gene92668:intron
CTGTAAAACTTGTTTCTGAA+AGG 0.534187 3.1:-23814070 MS.gene92668:CDS
CCTATCTCCTCAAAAGACAA+AGG 0.538706 3.1:+23813721 None:intergenic
TTAACCAGGAAGGAACCATT+GGG 0.543281 3.1:-23814925 MS.gene92668:intron
GGAATCATTTCAACTGGTCC+CGG 0.544959 3.1:+23813859 None:intergenic
TCAGCAATTTCATTATATGT+CGG 0.554776 3.1:+23814116 None:intergenic
AAAGAATGACAGAACATGAA+AGG 0.564645 3.1:-23815158 None:intergenic
ATAGGGAGAAAAGTATGGAA+TGG 0.569556 3.1:-23814192 MS.gene92668:intron
TGTTGAAACTATCAAGGGAG+AGG 0.571469 3.1:-23813693 MS.gene92668:CDS
GAGACAGGTAATGAAGGAAG+GGG 0.571902 3.1:-23813821 MS.gene92668:CDS
TGCTCCATGAAGAAGCCCAA+TGG 0.593800 3.1:+23814910 None:intergenic
TGTCTACATATGTTTACTGG+TGG 0.613609 3.1:-23814856 MS.gene92668:CDS
GTTTACTGGTGGATTAAACA+AGG 0.614224 3.1:-23814845 MS.gene92668:CDS
TCAGAGCAACTTAATACTGA+AGG 0.620679 3.1:-23814009 MS.gene92668:intron
ATAATGCAGGAGATTATACC+GGG 0.633370 3.1:-23813877 MS.gene92668:intron
AAAGCTGTTGAAACTATCAA+GGG 0.636294 3.1:-23813698 MS.gene92668:CDS
GACATATAATGAAATTGCTG+AGG 0.644481 3.1:-23814114 MS.gene92668:CDS
TATCTCCTCAAAAGACAAAG+GGG 0.686203 3.1:+23813723 None:intergenic
AGATTAGCTCGTGATTATCG+CGG 0.696504 3.1:-23815078 MS.gene92668:CDS
ATGTCTTAAGACGAAGACTG+AGG 0.725315 3.1:-23814145 MS.gene92668:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTATAACATTTTTTG+GGG - chr3.1:23814000-23814019 MS.gene92668:intron 10.0%
!!! TTTTTTATAACATTTTTTGG+GGG - chr3.1:23814001-23814020 MS.gene92668:intron 15.0%
!! AACTGTTTATTATGAATGTT+TGG - chr3.1:23814536-23814555 MS.gene92668:intron 20.0%
!! ATAAAAAAAATTTGTCTCAC+CGG + chr3.1:23813989-23814008 None:intergenic 20.0%
!! ATAAACCGGAAAAATAAAAT+TGG - chr3.1:23814431-23814450 MS.gene92668:intron 20.0%
!! GAGATTGTAATATTATACTT+TGG - chr3.1:23815005-23815024 MS.gene92668:intron 20.0%
!!! ACTATTTTACAACTATTCTA+AGG - chr3.1:23814065-23814084 MS.gene92668:CDS 20.0%
!!! AGTAACCAATTTTATTTTTC+CGG + chr3.1:23814439-23814458 None:intergenic 20.0%
!!! ATTTGTTAAAGTTTCTCATT+AGG - chr3.1:23813804-23813823 MS.gene92668:CDS 20.0%
!!! TACAATTTCATTCGTATTTT+AGG - chr3.1:23814782-23814801 MS.gene92668:intron 20.0%
!!! TGGTTAGTTTGTTATTTATA+GGG - chr3.1:23814556-23814575 MS.gene92668:intron 20.0%
!!! TTGGTTAGTTTGTTATTTAT+AGG - chr3.1:23814555-23814574 MS.gene92668:intron 20.0%
!!! TTTATTTTTCCGGTTTATAA+CGG + chr3.1:23814429-23814448 None:intergenic 20.0%
! AACCAATCATATGCTAAATT+TGG - chr3.1:23814217-23814236 MS.gene92668:intron 25.0%
! ATATTATACTTTGGACTCAA+TGG - chr3.1:23815014-23815033 MS.gene92668:CDS 25.0%
! TATTTATAGGGAGAAAAGTA+TGG - chr3.1:23814568-23814587 MS.gene92668:intron 25.0%
! TCAGCAATTTCATTATATGT+CGG + chr3.1:23814652-23814671 None:intergenic 25.0%
! TGTTTACTATGATATAATGC+AGG - chr3.1:23814875-23814894 MS.gene92668:CDS 25.0%
! TTTAATTCATTTAACCAGGA+AGG - chr3.1:23813830-23813849 MS.gene92668:CDS 25.0%
!!! GCATTTTAATTCATTTAACC+AGG - chr3.1:23813826-23813845 MS.gene92668:CDS 25.0%
AAAGCTGTTGAAACTATCAA+GGG - chr3.1:23815067-23815086 MS.gene92668:CDS 30.0%
AGTGATATACCGTTATAAAC+CGG - chr3.1:23814417-23814436 MS.gene92668:intron 30.0%
CACCAAATTTAGCATATGAT+TGG + chr3.1:23814222-23814241 None:intergenic 30.0%
CGTGAATTGTGAAAACTTAA+CGG + chr3.1:23814257-23814276 None:intergenic 30.0%
GACATATAATGAAATTGCTG+AGG - chr3.1:23814651-23814670 MS.gene92668:intron 30.0%
TATAATGCAGGAGATTATAC+CGG - chr3.1:23814887-23814906 MS.gene92668:CDS 30.0%
TTGATTAGACGAAGTTAGTT+AGG - chr3.1:23814292-23814311 MS.gene92668:intron 30.0%
! ACTTTTACTGACGTATGTTT+TGG + chr3.1:23814362-23814381 None:intergenic 30.0%
! CACTATTTTCATAGTCTCTT+TGG - chr3.1:23814462-23814481 MS.gene92668:intron 30.0%
!! CTGTAAAACTTGTTTCTGAA+AGG - chr3.1:23814695-23814714 MS.gene92668:intron 30.0%
AAAAACAACCATGTTGCTCA+AGG + chr3.1:23814186-23814205 None:intergenic 35.0%
AAGCATTCAAGATCTCATTC+AGG - chr3.1:23813752-23813771 MS.gene92668:CDS 35.0%
ACAAGGCATGATTAAAGCTT+TGG - chr3.1:23813937-23813956 MS.gene92668:intron 35.0%
AGCTGTCTACATATGTTTAC+TGG - chr3.1:23813906-23813925 MS.gene92668:intron 35.0%
ATAATGCAGGAGATTATACC+GGG - chr3.1:23814888-23814907 MS.gene92668:CDS 35.0%
CTATCTCCTCAAAAGACAAA+GGG + chr3.1:23815046-23815065 None:intergenic 35.0%
GAAAGCTGTTGAAACTATCA+AGG - chr3.1:23815066-23815085 MS.gene92668:CDS 35.0%
TAGACGAAGTTAGTTAGGTA+TGG - chr3.1:23814297-23814316 MS.gene92668:intron 35.0%
TATCTCCTCAAAAGACAAAG+GGG + chr3.1:23815045-23815064 None:intergenic 35.0%
TCAGAGCAACTTAATACTGA+AGG - chr3.1:23814756-23814775 MS.gene92668:intron 35.0%
TGTCTACATATGTTTACTGG+TGG - chr3.1:23813909-23813928 MS.gene92668:intron 35.0%
! ATAGCTCTGTCCAATGAAAT+TGG + chr3.1:23814730-23814749 None:intergenic 35.0%
! ATAGGGAGAAAAGTATGGAA+TGG - chr3.1:23814573-23814592 MS.gene92668:intron 35.0%
! TGTCTCTCAAACATTTTCTC+CGG + chr3.1:23814930-23814949 None:intergenic 35.0%
!! GATTTTAACCTTGAGCAACA+TGG - chr3.1:23814175-23814194 MS.gene92668:CDS 35.0%
!! GTTTACTGGTGGATTAAACA+AGG - chr3.1:23813920-23813939 MS.gene92668:intron 35.0%
AATGGTTGCAAAAATCGCAG+AGG - chr3.1:23814591-23814610 MS.gene92668:intron 40.0%
AGAGACAGGTAATGAAGGAA+GGG - chr3.1:23814943-23814962 MS.gene92668:intron 40.0%
ATGTCTTAAGACGAAGACTG+AGG - chr3.1:23814620-23814639 MS.gene92668:intron 40.0%
CCTATCTCCTCAAAAGACAA+AGG + chr3.1:23815047-23815066 None:intergenic 40.0%
GAGAAATTTGATCCGGATAG+AGG - chr3.1:23813878-23813897 MS.gene92668:intron 40.0%
GAGCAGACAACCAATTTCAT+TGG - chr3.1:23814717-23814736 MS.gene92668:intron 40.0%
GCAACACGTTATCAAGGAAA+AGG - chr3.1:23813726-23813745 MS.gene92668:CDS 40.0%
GGAGAAAATGTTTGAGAGAC+AGG - chr3.1:23814929-23814948 MS.gene92668:intron 40.0%
GTTTGAGAGACAGGTAATGA+AGG - chr3.1:23814938-23814957 MS.gene92668:intron 40.0%
TCAATCGCAACACGTTATCA+AGG - chr3.1:23813720-23813739 MS.gene92668:CDS 40.0%
TGGAGCAGAGAAATTTGATC+CGG - chr3.1:23813871-23813890 MS.gene92668:CDS 40.0%
TGTTGAAACTATCAAGGGAG+AGG - chr3.1:23815072-23815091 MS.gene92668:CDS 40.0%
TTAACCAGGAAGGAACCATT+GGG - chr3.1:23813840-23813859 MS.gene92668:CDS 40.0%
TTAGTTAGGTATGGTGCATG+CGG - chr3.1:23814306-23814325 MS.gene92668:intron 40.0%
TTCTCCGGAATCATTTCAAC+TGG + chr3.1:23814915-23814934 None:intergenic 40.0%
TTTAACCAGGAAGGAACCAT+TGG - chr3.1:23813839-23813858 MS.gene92668:CDS 40.0%
! AGATTAGCTCGTGATTATCG+CGG - chr3.1:23813687-23813706 MS.gene92668:CDS 40.0%
! GTCCAGATTAGTCAGATTAC+CGG - chr3.1:23813967-23813986 MS.gene92668:intron 40.0%
!! CCTTTGTCTTTTGAGGAGAT+AGG - chr3.1:23815044-23815063 MS.gene92668:CDS 40.0%
!!! ATAACATTTTTTGGGGGCGA+GGG - chr3.1:23814007-23814026 MS.gene92668:intron 40.0%
!!! TATAACATTTTTTGGGGGCG+AGG - chr3.1:23814006-23814025 MS.gene92668:intron 40.0%
CACCGGTAATCTGACTAATC+TGG + chr3.1:23813972-23813991 None:intergenic 45.0%
GACAGCTTACATCCTCTATC+CGG + chr3.1:23813893-23813912 None:intergenic 45.0%
GAGACAGGTAATGAAGGAAG+GGG - chr3.1:23814944-23814963 MS.gene92668:intron 45.0%
GAGAGACAGGTAATGAAGGA+AGG - chr3.1:23814942-23814961 MS.gene92668:intron 45.0%
GAGAGGATCAGACAGATTCA+TGG - chr3.1:23815089-23815108 MS.gene92668:CDS 45.0%
GGAATCATTTCAACTGGTCC+CGG + chr3.1:23814909-23814928 None:intergenic 45.0%
GGGACCAGTTGAAATGATTC+CGG - chr3.1:23814908-23814927 MS.gene92668:CDS 45.0%
TATTTGAACCGCGTCTCTTG+AGG - chr3.1:23814089-23814108 MS.gene92668:CDS 45.0%
! AGAGACCCCTTTGTCTTTTG+AGG - chr3.1:23815037-23815056 MS.gene92668:CDS 45.0%
!!! TAACATTTTTTGGGGGCGAG+GGG - chr3.1:23814008-23814027 MS.gene92668:intron 45.0%
!!! ATTTTTTTTATAACATTTTT+TGG - chr3.1:23813998-23814017 MS.gene92668:intron 5.0%
!!! TTTTTTTTATAACATTTTTT+GGG - chr3.1:23813999-23814018 MS.gene92668:intron 5.0%
CCTTGTAACCTCAAGAGACG+CGG + chr3.1:23814100-23814119 None:intergenic 50.0%
GGAACCATTGGGCTTCTTCA+TGG - chr3.1:23813851-23813870 MS.gene92668:CDS 50.0%
TGCTCCATGAAGAAGCCCAA+TGG + chr3.1:23813858-23813877 None:intergenic 50.0%
CCGCGTCTCTTGAGGTTACA+AGG - chr3.1:23814097-23814116 MS.gene92668:CDS 55.0%
GAAGCCCAATGGTTCCTTCC+TGG + chr3.1:23813847-23813866 None:intergenic 55.0%
GTTAGGTATGGTGCATGCGG+AGG - chr3.1:23814309-23814328 MS.gene92668:intron 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 23813612 23815175 23813612 ID=MS.gene92668
chr3.1 mRNA 23813612 23815175 23813612 ID=MS.gene92668.t1;Parent=MS.gene92668
chr3.1 exon 23815014 23815175 23815014 ID=MS.gene92668.t1.exon1;Parent=MS.gene92668.t1
chr3.1 CDS 23815014 23815175 23815014 ID=cds.MS.gene92668.t1;Parent=MS.gene92668.t1
chr3.1 exon 23814799 23814939 23814799 ID=MS.gene92668.t1.exon2;Parent=MS.gene92668.t1
chr3.1 CDS 23814799 23814939 23814799 ID=cds.MS.gene92668.t1;Parent=MS.gene92668.t1
chr3.1 exon 23814010 23814210 23814010 ID=MS.gene92668.t1.exon3;Parent=MS.gene92668.t1
chr3.1 CDS 23814010 23814210 23814010 ID=cds.MS.gene92668.t1;Parent=MS.gene92668.t1
chr3.1 exon 23813612 23813890 23813612 ID=MS.gene92668.t1.exon4;Parent=MS.gene92668.t1
chr3.1 CDS 23813612 23813890 23813612 ID=cds.MS.gene92668.t1;Parent=MS.gene92668.t1
Gene Sequence

>MS.gene92668

ATGACAGAACATGAAAGGAATTATGCTATTACAAGTCTAGATAAAGTGCTGATTAATACAAGACAATCAAAAGAAAGATTAGCTCGTGATTATCGCGGTTTATTTGCATCAATCGCAACACGTTATCAAGGAAAAGGCTTAAGCATTCAAGATCTCATTCAGGTGCATACTAACACATTTCAGTATTTTGATATTTGTTAAAGTTTCTCATTAGGCATTTTAATTCATTTAACCAGGAAGGAACCATTGGGCTTCTTCATGGAGCAGAGAAATTTGATCCGGATAGAGGATGTAAGCTGTCTACATATGTTTACTGGTGGATTAAACAAGGCATGATTAAAGCTTTGGCGAAGAAGTCCAGATTAGTCAGATTACCGGTGAGACAAATTTTTTTTATAACATTTTTTGGGGGCGAGGGGATGAAAATTATCTTTAGTTAAAGTGATATTAGTGACTATTTTACAACTATTCTAAGGATATTTGAACCGCGTCTCTTGAGGTTACAAGGTGAAGCTCTTACAAATGAGACGCTTATTCAATTGTTTACTAATAATGCTAAAAATGATTTTAACCTTGAGCAACATGGTTGTTTTTAGTTAGCTTTGAACCAATCATATGCTAAATTTGGTGTAAGTTCTTTAACCGTTAAGTTTTCACAATTCACGACTAATTTTTGACTTTTGATTAGACGAAGTTAGTTAGGTATGGTGCATGCGGAGGTTACTAGTGATTCTAGTTTTTAAAAAACCAAAACATACGTCAGTAAAAGTGATAAGTAAGATAGTTTAAAAAACTATAGTAAAATAGTGATATACCGTTATAAACCGGAAAAATAAAATTGGTTACTTATCACTATTTTCATAGTCTCTTTGGATTTACTATTATTGAAAGTGTTTTGATCAATAAACTATCTTACTATCAATAAACTGTTTATTATGAATGTTTGGTTAGTTTGTTATTTATAGGGAGAAAAGTATGGAATGGTTGCAAAAATCGCAGAGGCTAACAATGTCTTAAGACGAAGACTGAGGCAGAAGCCGACATATAATGAAATTGCTGAGGTTCTCAATGTGAATGTTTCAACTGTAAAACTTGTTTCTGAAAGGAGCAGACAACCAATTTCATTGGACAGAGCTATAACTGATCAGAGCAACTTAATACTGAAGGTTCTACAATTTCATTCGTATTTTAGGCGATTAACTACACATTCTTATTCTTATTATTATATATGAAAAGCTACAAACTCTGAGCTATTAGTCATAATGTTTACTATGATATAATGCAGGAGATTATACCGGGACCAGTTGAAATGATTCCGGAGAAAATGTTTGAGAGACAGGTAATGAAGGAAGGGGTTGTGAAGCTTCTTAACACACTTAACAAGAGAGAAGAAGAGATTGTAATATTATACTTTGGACTCAATGGAGAGACCCCTTTGTCTTTTGAGGAGATAGGAAAGCTGTTGAAACTATCAAGGGAGAGGATCAGACAGATTCATGGTATTGCATTGTCAACATTGCAACAAAACTCACTTGTAGATAGTCTAAAGTTTTATGTTGTATAG

Protein sequence

>MS.gene92668.t1

MTEHERNYAITSLDKVLINTRQSKERLARDYRGLFASIATRYQGKGLSIQDLIQEGTIGLLHGAEKFDPDRGCKLSTYVYWWIKQGMIKALAKKSRLVRLPGEKYGMVAKIAEANNVLRRRLRQKPTYNEIAEVLNVNVSTVKLVSERSRQPISLDRAITDQSNLILKEIIPGPVEMIPEKMFERQVMKEGVVKLLNTLNKREEEIVILYFGLNGETPLSFEEIGKLLKLSRERIRQIHGIALSTLQQNSLVDSLKFYVV