AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97613


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97613.t1 MTR_7g006040 98.684 228 3 0 1 228 167 394 1.20e-166 465
MS.gene97613.t1 MTR_8g064720 70.312 192 52 3 1 188 187 377 1.34e-86 262
MS.gene97613.t1 MTR_5g072900 38.824 170 89 5 33 189 219 386 8.90e-24 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97613.t1 AT1G18480 61.638 232 79 5 1 228 166 391 1.23e-91 275
MS.gene97613.t1 AT1G07010 36.471 170 95 4 33 190 204 372 4.45e-21 90.9
MS.gene97613.t1 AT1G07010 36.471 170 95 4 33 190 208 376 4.60e-21 90.9
MS.gene97613.t1 AT1G07010 36.471 170 95 4 33 190 219 387 5.97e-21 90.5

Find 70 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTAGGGTTGCGGCTTTA+AGG 0.182522 7.4:+93551264 MS.gene97613:CDS
TTGAAAGAACATGTTGATTA+TGG 0.215908 7.4:+93551374 MS.gene97613:CDS
TGAGGTTAGTGATTGGTATA+AGG 0.246230 7.4:+93551415 MS.gene97613:CDS
AAGAGTTTCATGATGGGTTT+AGG 0.248900 7.4:+93551243 MS.gene97613:CDS
TGGTGTTAAGAGGATGATTA+TGG 0.249368 7.4:+93551562 MS.gene97613:CDS
TGGTGGTTTGCCCGAGGTTT+TGG 0.263789 7.4:+93551670 MS.gene97613:CDS
ACGTGGCGTTTCGTGGTGTT+AGG 0.288942 7.4:+93551219 MS.gene97613:CDS
TTATTCATCTGATTATATAA+AGG 0.310568 7.4:-93551731 None:intergenic
TAATGGTGTCTGCGAAAATA+AGG 0.312538 7.4:+93551610 MS.gene97613:CDS
TACACAAAATGTTACTGTTT+TGG 0.322237 7.4:+93551316 MS.gene97613:CDS
TTTATATAATCAGATGAATA+AGG 0.349298 7.4:+93551733 MS.gene97613:CDS
GACAATAAGGCGGCGAAAAC+CGG 0.357356 7.4:-93551451 None:intergenic
GAGGTTAGTGATTGGTATAA+GGG 0.359259 7.4:+93551416 MS.gene97613:CDS
GATTGGTATAAGGGTTTGTT+TGG 0.360244 7.4:+93551425 MS.gene97613:CDS
GGGAAGGTGGAAGAAGGGTT+TGG 0.362537 7.4:+93551770 MS.gene97613:CDS
CGAATGGACCGATTTCAAAA+AGG 0.363226 7.4:+93551288 MS.gene97613:CDS
ATTCAGAAGGAAGGGATTAA+TGG 0.366442 7.4:+93551593 MS.gene97613:CDS
AGAGTTTCATGATGGGTTTA+GGG 0.373309 7.4:+93551244 MS.gene97613:CDS
GCATTCCTTCCCCGACAATA+AGG 0.375199 7.4:-93551464 None:intergenic
TTCATGATGGGTTTAGGGCT+AGG 0.377495 7.4:+93551249 MS.gene97613:CDS
GGGGAAGGAATGCACTTGTT+TGG 0.383176 7.4:+93551474 MS.gene97613:CDS
TATGGGTTGGAGAAGATTAA+TGG 0.389368 7.4:+93551392 MS.gene97613:CDS
TGAAAGAACATGTTGATTAT+GGG 0.411575 7.4:+93551375 MS.gene97613:CDS
ATAAGGGTTTGTTTGGTAAC+CGG 0.411872 7.4:+93551432 MS.gene97613:CDS
TCATGATGGGTTTAGGGCTA+GGG 0.424497 7.4:+93551250 MS.gene97613:CDS
CGACGATTCCTGGTGTTAAG+AGG 0.425093 7.4:+93551552 MS.gene97613:CDS
ATGTAAAGGTTTGGAAGAAA+AGG 0.436989 7.4:+93551172 MS.gene97613:CDS
CGTGGCGTTTCGTGGTGTTA+GGG 0.450367 7.4:+93551220 MS.gene97613:CDS
CGCCGCCTTATTGTCGGGGA+AGG 0.455393 7.4:+93551459 MS.gene97613:CDS
AATGTTCACGTGGCGTTTCG+TGG 0.456234 7.4:+93551212 MS.gene97613:CDS
TCTGCGAAAATAAGGCGATT+AGG 0.459167 7.4:+93551618 MS.gene97613:CDS
GATGTTGGGAAGGTGGAAGA+AGG 0.466879 7.4:+93551764 MS.gene97613:CDS
AATAAGGAAAATGTGGATGT+TGG 0.471510 7.4:+93551749 MS.gene97613:CDS
TGGGTTTAGGGCTAGGGTTG+CGG 0.472943 7.4:+93551256 MS.gene97613:CDS
GGTGTTAAGAGGATGATTAT+GGG 0.476200 7.4:+93551563 MS.gene97613:CDS
AAATGTGGATGTTGGGAAGG+TGG 0.504316 7.4:+93551757 MS.gene97613:CDS
TCAGATGAATAAGGAAAATG+TGG 0.505988 7.4:+93551742 MS.gene97613:CDS
GTTAGGGAAGAGTTTCATGA+TGG 0.506126 7.4:+93551236 MS.gene97613:CDS
ATGTTGGGAAGGTGGAAGAA+GGG 0.512967 7.4:+93551765 MS.gene97613:CDS
AGATTGACCGTTATGGGGTG+AGG 0.524683 7.4:+93551693 MS.gene97613:CDS
GCCTTGACTTCCACTTGCCT+AGG 0.528320 7.4:-93551821 None:intergenic
GCAAGTGGAAGTCAAGGCTT+AGG 0.533119 7.4:+93551826 MS.gene97613:CDS
ATGTCGAAAGGATGCGGTGG+TGG 0.533822 7.4:+93551653 MS.gene97613:CDS
CGGTCAATCTCCAAAACCTC+GGG 0.534492 7.4:-93551680 None:intergenic
TTAATGGTGAGGTTAGTGAT+TGG 0.539710 7.4:+93551408 MS.gene97613:CDS
ATAAGGAAAATGTGGATGTT+GGG 0.548289 7.4:+93551750 MS.gene97613:CDS
ACCTAGGCAAGTGGAAGTCA+AGG 0.549753 7.4:+93551820 MS.gene97613:CDS
TTAGGGAAGAGTTTCATGAT+GGG 0.558605 7.4:+93551237 MS.gene97613:CDS
ATGCGGTGGTGGTTTGCCCG+AGG 0.563566 7.4:+93551664 MS.gene97613:CDS
TTGTTGCTTAATAATCAAGA+TGG 0.574078 7.4:+93551794 MS.gene97613:CDS
ATCGATGTTGGTATGTCGAA+AGG 0.575221 7.4:+93551641 MS.gene97613:CDS
GTTGGAGAAGATTAATGGTG+AGG 0.579243 7.4:+93551397 MS.gene97613:CDS
GGGCATACGATTCAGAAGGA+AGG 0.584494 7.4:+93551584 MS.gene97613:CDS
TCAATATCCTCACCCCATAA+CGG 0.588832 7.4:-93551700 None:intergenic
ACGGTCAATCTCCAAAACCT+CGG 0.600316 7.4:-93551681 None:intergenic
GGCATACGATTCAGAAGGAA+GGG 0.601131 7.4:+93551585 MS.gene97613:CDS
AGAACATGTTGATTATGGGT+TGG 0.606486 7.4:+93551379 MS.gene97613:CDS
AAGGCGATTAGGATCGATGT+TGG 0.608483 7.4:+93551629 MS.gene97613:CDS
GGAAAATGTGGATGTTGGGA+AGG 0.610345 7.4:+93551754 MS.gene97613:CDS
ATAATCATCCTCTTAACACC+AGG 0.619952 7.4:-93551560 None:intergenic
GTTGCGGCTTTAAGGCCGAA+TGG 0.620496 7.4:+93551272 MS.gene97613:CDS
TTTGGAGATTGACCGTTATG+GGG 0.621026 7.4:+93551688 MS.gene97613:CDS
TATGGGGCATACGATTCAGA+AGG 0.628721 7.4:+93551580 MS.gene97613:CDS
ATAATCAAGATGGTAGACCT+AGG 0.629740 7.4:+93551804 MS.gene97613:CDS
GTGTTAAGAGGATGATTATG+GGG 0.631883 7.4:+93551564 MS.gene97613:CDS
AGATGGTAGACCTAGGCAAG+TGG 0.641426 7.4:+93551811 MS.gene97613:CDS
GTTGGTATGTCGAAAGGATG+CGG 0.642403 7.4:+93551647 MS.gene97613:CDS
TTCCTTCCCCGACAATAAGG+CGG 0.649751 7.4:-93551461 None:intergenic
TCCATTAGAAAATGTTCACG+TGG 0.684935 7.4:+93551202 MS.gene97613:CDS
GGTATGTCGAAAGGATGCGG+TGG 0.743976 7.4:+93551650 MS.gene97613:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTCATCTGATTATATAA+AGG - chr7.4:93551734-93551753 None:intergenic 15.0%
!! TTTATATAATCAGATGAATA+AGG + chr7.4:93551733-93551752 MS.gene97613:CDS 15.0%
! GAAAAATCTATGTAAAGGTT+TGG + chr7.4:93551163-93551182 MS.gene97613:CDS 25.0%
!! TACACAAAATGTTACTGTTT+TGG + chr7.4:93551316-93551335 MS.gene97613:CDS 25.0%
!! TGAAAGAACATGTTGATTAT+GGG + chr7.4:93551375-93551394 MS.gene97613:CDS 25.0%
!! TTGAAAGAACATGTTGATTA+TGG + chr7.4:93551374-93551393 MS.gene97613:CDS 25.0%
!! TTGTTGCTTAATAATCAAGA+TGG + chr7.4:93551794-93551813 MS.gene97613:CDS 25.0%
!!! GTAAAGAACCTTTTTGAAAT+CGG - chr7.4:93551299-93551318 None:intergenic 25.0%
AATAAGGAAAATGTGGATGT+TGG + chr7.4:93551749-93551768 MS.gene97613:CDS 30.0%
ATAAGGAAAATGTGGATGTT+GGG + chr7.4:93551750-93551769 MS.gene97613:CDS 30.0%
ATGTAAAGGTTTGGAAGAAA+AGG + chr7.4:93551172-93551191 MS.gene97613:CDS 30.0%
TCAGATGAATAAGGAAAATG+TGG + chr7.4:93551742-93551761 MS.gene97613:CDS 30.0%
! GATTCAATTTTTGTTCATGG+TGG + chr7.4:93551347-93551366 MS.gene97613:CDS 30.0%
! GGCTTAGAAAATTTTCAGAT+GGG + chr7.4:93551495-93551514 MS.gene97613:CDS 30.0%
! GGTGATTCAATTTTTGTTCA+TGG + chr7.4:93551344-93551363 MS.gene97613:CDS 30.0%
! TGGCTTAGAAAATTTTCAGA+TGG + chr7.4:93551494-93551513 MS.gene97613:CDS 30.0%
!!! AATGTTACTGTTTTGGTAGT+TGG + chr7.4:93551323-93551342 MS.gene97613:CDS 30.0%
ATAATCAAGATGGTAGACCT+AGG + chr7.4:93551804-93551823 MS.gene97613:CDS 35.0%
ATAATCATCCTCTTAACACC+AGG - chr7.4:93551563-93551582 None:intergenic 35.0%
ATTCAGAAGGAAGGGATTAA+TGG + chr7.4:93551593-93551612 MS.gene97613:CDS 35.0%
GAGGTTAGTGATTGGTATAA+GGG + chr7.4:93551416-93551435 MS.gene97613:CDS 35.0%
GGTGTTAAGAGGATGATTAT+GGG + chr7.4:93551563-93551582 MS.gene97613:CDS 35.0%
GTGTTAAGAGGATGATTATG+GGG + chr7.4:93551564-93551583 MS.gene97613:CDS 35.0%
TAATGGTGTCTGCGAAAATA+AGG + chr7.4:93551610-93551629 MS.gene97613:CDS 35.0%
TCCATTAGAAAATGTTCACG+TGG + chr7.4:93551202-93551221 MS.gene97613:CDS 35.0%
TGAGGTTAGTGATTGGTATA+AGG + chr7.4:93551415-93551434 MS.gene97613:CDS 35.0%
TTAATGGTGAGGTTAGTGAT+TGG + chr7.4:93551408-93551427 MS.gene97613:CDS 35.0%
TTAGGGAAGAGTTTCATGAT+GGG + chr7.4:93551237-93551256 MS.gene97613:CDS 35.0%
! AGAACATGTTGATTATGGGT+TGG + chr7.4:93551379-93551398 MS.gene97613:CDS 35.0%
! ATAAGGGTTTGTTTGGTAAC+CGG + chr7.4:93551432-93551451 MS.gene97613:CDS 35.0%
! GATTGGTATAAGGGTTTGTT+TGG + chr7.4:93551425-93551444 MS.gene97613:CDS 35.0%
! TGGTGTTAAGAGGATGATTA+TGG + chr7.4:93551562-93551581 MS.gene97613:CDS 35.0%
!! AAGAGTTTCATGATGGGTTT+AGG + chr7.4:93551243-93551262 MS.gene97613:CDS 35.0%
!! AGAGTTTCATGATGGGTTTA+GGG + chr7.4:93551244-93551263 MS.gene97613:CDS 35.0%
!! TATGGGTTGGAGAAGATTAA+TGG + chr7.4:93551392-93551411 MS.gene97613:CDS 35.0%
!! TTTTGGAGATTGACCGTTAT+GGG + chr7.4:93551687-93551706 MS.gene97613:CDS 35.0%
!!! CTTTTTGAAATCGGTCCATT+CGG - chr7.4:93551290-93551309 None:intergenic 35.0%
ATCGATGTTGGTATGTCGAA+AGG + chr7.4:93551641-93551660 MS.gene97613:CDS 40.0%
GTTAGGGAAGAGTTTCATGA+TGG + chr7.4:93551236-93551255 MS.gene97613:CDS 40.0%
GTTGGAGAAGATTAATGGTG+AGG + chr7.4:93551397-93551416 MS.gene97613:CDS 40.0%
TCAATATCCTCACCCCATAA+CGG - chr7.4:93551703-93551722 None:intergenic 40.0%
TCTGCGAAAATAAGGCGATT+AGG + chr7.4:93551618-93551637 MS.gene97613:CDS 40.0%
TTTGGAGATTGACCGTTATG+GGG + chr7.4:93551688-93551707 MS.gene97613:CDS 40.0%
! CGAATGGACCGATTTCAAAA+AGG + chr7.4:93551288-93551307 MS.gene97613:CDS 40.0%
! GCCACGTGAACATTTTCTAA+TGG - chr7.4:93551206-93551225 None:intergenic 40.0%
!!! GTTTTGGAGATTGACCGTTA+TGG + chr7.4:93551686-93551705 MS.gene97613:CDS 40.0%
AAATGTGGATGTTGGGAAGG+TGG + chr7.4:93551757-93551776 MS.gene97613:CDS 45.0%
AAGGCGATTAGGATCGATGT+TGG + chr7.4:93551629-93551648 MS.gene97613:CDS 45.0%
ACGGTCAATCTCCAAAACCT+CGG - chr7.4:93551684-93551703 None:intergenic 45.0%
ATGTTGGGAAGGTGGAAGAA+GGG + chr7.4:93551765-93551784 MS.gene97613:CDS 45.0%
GGAAAATGTGGATGTTGGGA+AGG + chr7.4:93551754-93551773 MS.gene97613:CDS 45.0%
GGCATACGATTCAGAAGGAA+GGG + chr7.4:93551585-93551604 MS.gene97613:CDS 45.0%
GTTGGTATGTCGAAAGGATG+CGG + chr7.4:93551647-93551666 MS.gene97613:CDS 45.0%
TATGGGGCATACGATTCAGA+AGG + chr7.4:93551580-93551599 MS.gene97613:CDS 45.0%
! CATGTTTTGTCGACGATTCC+TGG + chr7.4:93551542-93551561 MS.gene97613:CDS 45.0%
!! TCATGATGGGTTTAGGGCTA+GGG + chr7.4:93551250-93551269 MS.gene97613:CDS 45.0%
!! TTCATGATGGGTTTAGGGCT+AGG + chr7.4:93551249-93551268 MS.gene97613:CDS 45.0%
AATGTTCACGTGGCGTTTCG+TGG + chr7.4:93551212-93551231 MS.gene97613:CDS 50.0%
ACCTAGGCAAGTGGAAGTCA+AGG + chr7.4:93551820-93551839 MS.gene97613:CDS 50.0%
AGATGGTAGACCTAGGCAAG+TGG + chr7.4:93551811-93551830 MS.gene97613:CDS 50.0%
AGATTGACCGTTATGGGGTG+AGG + chr7.4:93551693-93551712 MS.gene97613:CDS 50.0%
CGGTCAATCTCCAAAACCTC+GGG - chr7.4:93551683-93551702 None:intergenic 50.0%
GACAATAAGGCGGCGAAAAC+CGG - chr7.4:93551454-93551473 None:intergenic 50.0%
GATGTTGGGAAGGTGGAAGA+AGG + chr7.4:93551764-93551783 MS.gene97613:CDS 50.0%
GCATTCCTTCCCCGACAATA+AGG - chr7.4:93551467-93551486 None:intergenic 50.0%
GGGCATACGATTCAGAAGGA+AGG + chr7.4:93551584-93551603 MS.gene97613:CDS 50.0%
GGGGAAGGAATGCACTTGTT+TGG + chr7.4:93551474-93551493 MS.gene97613:CDS 50.0%
TTCCTTCCCCGACAATAAGG+CGG - chr7.4:93551464-93551483 None:intergenic 50.0%
! CGACGATTCCTGGTGTTAAG+AGG + chr7.4:93551552-93551571 MS.gene97613:CDS 50.0%
!! GGTTTTCGCCGCCTTATTGT+CGG + chr7.4:93551453-93551472 MS.gene97613:CDS 50.0%
!! GTTTTCGCCGCCTTATTGTC+GGG + chr7.4:93551454-93551473 MS.gene97613:CDS 50.0%
!! TTTTCGCCGCCTTATTGTCG+GGG + chr7.4:93551455-93551474 MS.gene97613:CDS 50.0%
ACGTGGCGTTTCGTGGTGTT+AGG + chr7.4:93551219-93551238 MS.gene97613:CDS 55.0%
ATGTCGAAAGGATGCGGTGG+TGG + chr7.4:93551653-93551672 MS.gene97613:CDS 55.0%
GCCTTGACTTCCACTTGCCT+AGG - chr7.4:93551824-93551843 None:intergenic 55.0%
GGGAAGGTGGAAGAAGGGTT+TGG + chr7.4:93551770-93551789 MS.gene97613:CDS 55.0%
GGTATGTCGAAAGGATGCGG+TGG + chr7.4:93551650-93551669 MS.gene97613:CDS 55.0%
! CGTGGCGTTTCGTGGTGTTA+GGG + chr7.4:93551220-93551239 MS.gene97613:CDS 55.0%
! TGGGTTTAGGGCTAGGGTTG+CGG + chr7.4:93551256-93551275 MS.gene97613:CDS 55.0%
!! GTTGCGGCTTTAAGGCCGAA+TGG + chr7.4:93551272-93551291 MS.gene97613:CDS 55.0%
!! TGGTGGTTTGCCCGAGGTTT+TGG + chr7.4:93551670-93551689 MS.gene97613:CDS 55.0%
GGGCTAGGGTTGCGGCTTTA+AGG + chr7.4:93551264-93551283 MS.gene97613:CDS 60.0%
! ATGCGGTGGTGGTTTGCCCG+AGG + chr7.4:93551664-93551683 MS.gene97613:CDS 65.0%
! CGCCGCCTTATTGTCGGGGA+AGG + chr7.4:93551459-93551478 MS.gene97613:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 93551161 93551847 93551161 ID=MS.gene97613
chr7.4 mRNA 93551161 93551847 93551161 ID=MS.gene97613.t1;Parent=MS.gene97613
chr7.4 exon 93551161 93551847 93551161 ID=MS.gene97613.t1.exon1;Parent=MS.gene97613.t1
chr7.4 CDS 93551161 93551847 93551161 ID=cds.MS.gene97613.t1;Parent=MS.gene97613.t1
Gene Sequence

>MS.gene97613

ATGAAAAATCTATGTAAAGGTTTGGAAGAAAAGGTTGTAGATCCATTAGAAAATGTTCACGTGGCGTTTCGTGGTGTTAGGGAAGAGTTTCATGATGGGTTTAGGGCTAGGGTTGCGGCTTTAAGGCCGAATGGACCGATTTCAAAAAGGTTCTTTACACAAAATGTTACTGTTTTGGTAGTTGGTGATTCAATTTTTGTTCATGGTGGTTTGTTGAAAGAACATGTTGATTATGGGTTGGAGAAGATTAATGGTGAGGTTAGTGATTGGTATAAGGGTTTGTTTGGTAACCGGTTTTCGCCGCCTTATTGTCGGGGAAGGAATGCACTTGTTTGGCTTAGAAAATTTTCAGATGGGAGTTGTGATTGTTCGAGTCTTGAGCATGTTTTGTCGACGATTCCTGGTGTTAAGAGGATGATTATGGGGCATACGATTCAGAAGGAAGGGATTAATGGTGTCTGCGAAAATAAGGCGATTAGGATCGATGTTGGTATGTCGAAAGGATGCGGTGGTGGTTTGCCCGAGGTTTTGGAGATTGACCGTTATGGGGTGAGGATATTGACATCAAATCCTTTATATAATCAGATGAATAAGGAAAATGTGGATGTTGGGAAGGTGGAAGAAGGGTTTGGATTGTTGCTTAATAATCAAGATGGTAGACCTAGGCAAGTGGAAGTCAAGGCTTAG

Protein sequence

>MS.gene97613.t1

MKNLCKGLEEKVVDPLENVHVAFRGVREEFHDGFRARVAALRPNGPISKRFFTQNVTVLVVGDSIFVHGGLLKEHVDYGLEKINGEVSDWYKGLFGNRFSPPYCRGRNALVWLRKFSDGSCDCSSLEHVLSTIPGVKRMIMGHTIQKEGINGVCENKAIRIDVGMSKGCGGGLPEVLEIDRYGVRILTSNPLYNQMNKENVDVGKVEEGFGLLLNNQDGRPRQVEVKA