AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080047791.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080047791.01.T01 MTR_4g134900 81.818 44 8 0 93 136 20 63 1.10e-17 72.4
MsG0080047791.01.T01 MTR_4g134900 75.000 44 7 1 1 44 1 40 4.99e-15 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080047791.01.T01 AT1G09794 63.636 44 12 1 1 44 1 40 9.25e-12 57.0

Find 16 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGCAATTCAATAACTTTA+AGG 0.154340 contig103end:-18209 None:intergenic
TATGTTAGTGTTAATATAAT+TGG 0.164452 contig103end:+17477 MsG0080047791.01.T01:intron
CCTTAAAGTTATTGAATTGC+TGG 0.305156 contig103end:+18209 MsG0080047791.01.T01:CDS
TTGCGGGAGAAAGTTGTTCT+TGG 0.356092 contig103end:-17276 None:intergenic
ATAGCTTGGTTGGTGAGTTA+AGG 0.376035 contig103end:-18112 None:intergenic
TAGCTTGGTTGGTGAGTTAA+GGG 0.388206 contig103end:-18111 None:intergenic
ACTCACTGTGCTTCGCTCTC+TGG 0.391788 contig103end:+18264 MsG0080047791.01.T01:CDS
ATGTTAGTGTTAATATAATT+GGG 0.403039 contig103end:+17478 MsG0080047791.01.T01:intron
AGTATAATTCATCACTTACT+TGG 0.421958 contig103end:-18304 None:intergenic
CATTGTTGAAACAAAACTGT+TGG 0.465328 contig103end:+18145 MsG0080047791.01.T01:CDS
CAAAGAGCCTTGCAAGAAAG+AGG 0.485000 contig103end:+17229 MsG0080047791.01.T01:CDS
TTACAAGCCTCTTTCTTGCA+AGG 0.522587 contig103end:-17236 None:intergenic
AAGGTTTGAGAAAATGGGGC+AGG 0.531461 contig103end:+17202 None:intergenic
AAGTTTCTTATAATACTGCT+AGG 0.583345 contig103end:+18184 MsG0080047791.01.T01:CDS
GGAGAAAGTTGTTCTTGGAG+AGG 0.604134 contig103end:-17271 None:intergenic
ACAGTCTGACAGGAATGAAG+AGG 0.637824 contig103end:-17516 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATAAGCAAATAT+TGG + contig103end:18017-18036 MsG0080047791.01.T01:intron 10.0%
!! AAAATCCCTAATAATATAAT+TGG + contig103end:17401-17420 MsG0080047791.01.T01:intron 15.0%
!! AATTATATTAACACTAACAT+AGG - contig103end:17478-17497 None:intergenic 15.0%
!! ATGTTAGTGTTAATATAATT+GGG + contig103end:17478-17497 MsG0080047791.01.T01:intron 15.0%
!! TATGTTAGTGTTAATATAAT+TGG + contig103end:17477-17496 MsG0080047791.01.T01:intron 15.0%
!! TCAATCCAATTATATTATTA+GGG - contig103end:17409-17428 None:intergenic 15.0%
!! TTCAATCCAATTATATTATT+AGG - contig103end:17410-17429 None:intergenic 15.0%
!!! TTAATGGTTATGTTAATTTT+TGG + contig103end:18044-18063 MsG0080047791.01.T01:intron 15.0%
!! AAAATTCCAGTTTCTAATAT+TGG - contig103end:18002-18021 None:intergenic 20.0%
!! CAAAACAAATCAAATGAAAT+AGG - contig103end:17444-17463 None:intergenic 20.0%
!! TGACATAGTTAATTATAAGT+TGG + contig103end:17551-17570 MsG0080047791.01.T01:intron 20.0%
!!! GTTTTTAATTAAAACAACCA+CGG + contig103end:17743-17762 MsG0080047791.01.T01:intron 20.0%
!!! TTAATTTTTGGAATTGAACT+CGG + contig103end:18056-18075 MsG0080047791.01.T01:intron 20.0%
! AAGTTTCTTATAATACTGCT+AGG + contig103end:18184-18203 MsG0080047791.01.T01:CDS 25.0%
! AGCAAATATTGGTAAGTTAA+TGG + contig103end:18028-18047 MsG0080047791.01.T01:intron 25.0%
! TAAAGAATACTTGAACACTA+CGG + contig103end:17821-17840 MsG0080047791.01.T01:intron 25.0%
! TATATCACAATCAAAATCAG+TGG - contig103end:17377-17396 None:intergenic 25.0%
! TATTTAATTAACAGTCTGAC+AGG - contig103end:17529-17548 None:intergenic 25.0%
!! AAACAAAAACATACTTTTGC+GGG - contig103end:17295-17314 None:intergenic 25.0%
!! AATGTTTTGTAAAGATAGCT+TGG - contig103end:18129-18148 None:intergenic 25.0%
!! CAAACAAAAACATACTTTTG+CGG - contig103end:17296-17315 None:intergenic 25.0%
!!! GTTTTCTAACTTTTCTAAGA+AGG - contig103end:17687-17706 None:intergenic 25.0%
AGTTAGAAAACTTTCTTCTG+CGG + contig103end:17696-17715 MsG0080047791.01.T01:intron 30.0%
CATTGTTGAAACAAAACTGT+TGG + contig103end:18145-18164 MsG0080047791.01.T01:CDS 30.0%
CCAGCAATTCAATAACTTTA+AGG - contig103end:18212-18231 None:intergenic 30.0%
CCTTAAAGTTATTGAATTGC+TGG + contig103end:18209-18228 MsG0080047791.01.T01:CDS 30.0%
! TTTTGTAAAGATAGCTTGGT+TGG - contig103end:18125-18144 None:intergenic 30.0%
ACAACGCCAATATTAGAAAC+TGG + contig103end:17993-18012 MsG0080047791.01.T01:intron 35.0%
CAAATGATGCAAATGTCAGA+AGG + contig103end:17929-17948 MsG0080047791.01.T01:intron 35.0%
CTTCTGACATTTGCATCATT+TGG - contig103end:17931-17950 None:intergenic 35.0%
TATGCCGGAATAGAGTTTAA+TGG - contig103end:17637-17656 None:intergenic 35.0%
TCCACCATTAAACTCTATTC+CGG + contig103end:17630-17649 MsG0080047791.01.T01:intron 35.0%
!!! TTTTGGAATTGAACTCGGAA+GGG + contig103end:18061-18080 MsG0080047791.01.T01:intron 35.0%
!!! TTTTTGGAATTGAACTCGGA+AGG + contig103end:18060-18079 MsG0080047791.01.T01:intron 35.0%
GCACAGTGGTATCATCTAAA+TGG + contig103end:17953-17972 MsG0080047791.01.T01:intron 40.0%
TCTAAATGGCTATGCCTATG+CGG + contig103end:17967-17986 MsG0080047791.01.T01:intron 40.0%
TTACAAGCCTCTTTCTTGCA+AGG - contig103end:17239-17258 None:intergenic 40.0%
!! ATAGCTTGGTTGGTGAGTTA+AGG - contig103end:18115-18134 None:intergenic 40.0%
!! TAGCTTGGTTGGTGAGTTAA+GGG - contig103end:18114-18133 None:intergenic 40.0%
AAAACTCTCTTCCTGGTGAC+TGG + contig103end:17784-17803 MsG0080047791.01.T01:intron 45.0%
ACAGTCTGACAGGAATGAAG+AGG - contig103end:17519-17538 None:intergenic 45.0%
CAAAGAGCCTTGCAAGAAAG+AGG + contig103end:17229-17248 MsG0080047791.01.T01:CDS 45.0%
CCTACCAAACACCTTTCATG+AGG - contig103end:17724-17743 None:intergenic 45.0%
GCCGGAATAGAGTTTAATGG+TGG - contig103end:17634-17653 None:intergenic 45.0%
GGAGAAAGTTGTTCTTGGAG+AGG - contig103end:17274-17293 None:intergenic 45.0%
GTGGATATGCAGAAGCCATT+GGG - contig103end:17615-17634 None:intergenic 45.0%
TTGCGGGAGAAAGTTGTTCT+TGG - contig103end:17279-17298 None:intergenic 45.0%
! AAATGTCAGAAGGTGCACAG+TGG + contig103end:17939-17958 MsG0080047791.01.T01:intron 45.0%
! AAGGAGTGCGAAAACAGTCT+CGG - contig103end:17668-17687 None:intergenic 45.0%
!! CCTCATGAAAGGTGTTTGGT+AGG + contig103end:17721-17740 MsG0080047791.01.T01:intron 45.0%
ATTGGCGTTGTGTTCCGCAT+AGG - contig103end:17984-18003 None:intergenic 50.0%
GCGCTGAAAAACTCTCTTCC+TGG + contig103end:17777-17796 MsG0080047791.01.T01:intron 50.0%
GGTGGATATGCAGAAGCCAT+TGG - contig103end:17616-17635 None:intergenic 50.0%
ACTCACTGTGCTTCGCTCTC+TGG + contig103end:18264-18283 MsG0080047791.01.T01:CDS 55.0%
CTCTGATCTCACCAGTCACC+AGG - contig103end:17798-17817 None:intergenic 55.0%
GAGTGCGAAAACAGTCTCGG+AGG - contig103end:17665-17684 None:intergenic 55.0%
GCAGAAGCCATTGGGACAGA+GGG - contig103end:17607-17626 None:intergenic 55.0%
GCGAAAACAGTCTCGGAGGT+TGG - contig103end:17661-17680 None:intergenic 55.0%
TGCAGAAGCCATTGGGACAG+AGG - contig103end:17608-17627 None:intergenic 55.0%
TGTAACGCCCTCTGTCCCAA+TGG + contig103end:17597-17616 MsG0080047791.01.T01:intron 55.0%
! GTCTCGGAGGTTGGTTATGC+CGG - contig103end:17652-17671 None:intergenic 55.0%
!! GGCGCCTCATGAAAGGTGTT+TGG + contig103end:17717-17736 MsG0080047791.01.T01:intron 55.0%
CAGCGCAACTGTGATGTCCG+TGG - contig103end:17763-17782 None:intergenic 60.0%
CTTCTGCGGCGCCTCATGAA+AGG + contig103end:17710-17729 MsG0080047791.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
contig103end gene 17215 18311 17215 ID=MsG0080047791.01;Name=MsG0080047791.01
contig103end mRNA 17215 18311 17215 ID=MsG0080047791.01.T01;Parent=MsG0080047791.01;Name=MsG0080047791.01.T01;_AED=0.39;_eAED=0.61;_QI=0|0|0|1|0|0|3|0|136
contig103end exon 17215 17351 17215 ID=MsG0080047791.01.T01:exon:2952;Parent=MsG0080047791.01.T01
contig103end exon 17485 17528 17485 ID=MsG0080047791.01.T01:exon:2951;Parent=MsG0080047791.01.T01
contig103end exon 18082 18311 18082 ID=MsG0080047791.01.T01:exon:2950;Parent=MsG0080047791.01.T01
contig103end CDS 17215 17351 17215 ID=MsG0080047791.01.T01:cds;Parent=MsG0080047791.01.T01
contig103end CDS 17485 17528 17485 ID=MsG0080047791.01.T01:cds;Parent=MsG0080047791.01.T01
contig103end CDS 18082 18311 18082 ID=MsG0080047791.01.T01:cds;Parent=MsG0080047791.01.T01
Gene Sequence

>MsG0080047791.01.T01

ATGGGGCAGGAAAGCAAAGAGCCTTGCAAGAAAGAGGCTTGTAACATTCAAGCTTGCCTCTCCAAGAACAACTTTCTCCCGCAAAAGTATGTTTTTGTTTGTTTCGATTTTATTTATTTAATTTCTGTTTGCATATATGTTAATATAATTGGGTTAGAAGATCAATTACCTCTTCATTCCTGGATCTCGTGCTTAAAACTTCTTGAGTGTCCCTTAACTCACCAACCAAGCTATCTTTACAAAACATTGTTGAAACAAAACTGTTGGACTGAGTTGCTGTCTGAAGTTTCTTATAATACTGCTAGGTGCCTTAAAGTTATTGAATTGCTGGACTCTTGCTGCTCAAGATGCGATTATAATTCAACTCACTGTGCTTCGCTCTCTGGACTATTGAAGCAAAATTCCAAGTAA

Protein sequence

>MsG0080047791.01.T01

MGQESKEPCKKEACNIQACLSKNNFLPQKYVFVCFDFIYLISVCIYVNIIGLEDQLPLHSWISCLKLLECPLTHQPSYLYKTLLKQNCWTELLSEVSYNTARCLKVIELLDSCCSRCDYNSTHCASLSGLLKQNSK*