AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027605.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027605.01.T01 MTR_5g062370 96.714 213 7 0 1 213 375 587 9.12e-147 421
MsG0580027605.01.T01 MTR_5g062370 96.714 213 7 0 1 213 375 587 1.91e-144 422
MsG0580027605.01.T01 MTR_5g062320 95.775 213 9 0 1 213 373 585 1.02e-142 417
MsG0580027605.01.T01 MTR_5g062430 92.958 213 15 0 1 213 375 587 1.97e-137 403
MsG0580027605.01.T01 MTR_5g062650 76.168 214 50 1 1 213 377 590 1.17e-110 334
MsG0580027605.01.T01 MTR_3g074060 70.423 213 62 1 2 213 369 581 2.23e-100 307
MsG0580027605.01.T01 MTR_7g109390 50.698 215 102 3 1 213 359 571 3.76e-63 209
MsG0580027605.01.T01 MTR_1g087240 49.302 215 105 3 1 213 354 566 1.23e-61 205
MsG0580027605.01.T01 MTR_2g008240 51.869 214 98 4 1 212 375 585 6.86e-61 203
MsG0580027605.01.T01 MTR_5g030860 49.074 216 104 4 1 213 370 582 2.21e-60 202
MsG0580027605.01.T01 MTR_4g094285 48.131 214 106 4 1 212 374 584 1.01e-58 197
MsG0580027605.01.T01 MTR_1g106860 51.628 215 99 4 2 213 361 573 3.39e-57 193
MsG0580027605.01.T01 MTR_2g034720 47.196 214 107 4 1 212 359 568 9.60e-55 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027605.01.T01 AT1G78060 60.000 215 82 3 1 213 363 575 1.92e-78 250
MsG0580027605.01.T01 AT5G09700 53.271 214 96 3 1 212 120 331 6.93e-69 219
MsG0580027605.01.T01 AT5G10560 52.558 215 99 2 1 213 380 593 9.17e-69 224
MsG0580027605.01.T01 AT5G09730 52.804 214 95 4 1 212 369 578 1.74e-63 210
MsG0580027605.01.T01 AT5G64570 51.643 213 99 4 1 212 379 588 7.88e-63 206
MsG0580027605.01.T01 AT5G64570 51.643 213 99 4 1 212 221 430 1.30e-62 205
MsG0580027605.01.T01 AT5G64570 51.643 213 99 4 1 212 379 588 9.80e-62 205
MsG0580027605.01.T01 AT1G02640 50.463 216 101 5 1 213 361 573 1.64e-61 204
MsG0580027605.01.T01 AT3G19620 50.926 216 99 6 1 212 362 574 3.15e-61 204
MsG0580027605.01.T01 AT5G49360 49.074 216 102 6 1 213 367 577 2.13e-54 185

Find 49 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAACTATTGATGATTTAT+TGG 0.145046 5:-64124132 None:intergenic
AATAAGTGATTATGTTATTT+TGG 0.156153 5:+64124304 MsG0580027605.01.T01:CDS
TTCTAAGAAGCCTGTTATTT+TGG 0.174390 5:+64124427 MsG0580027605.01.T01:CDS
GCTAAGAATAATGACAAAAT+TGG 0.212120 5:+64124485 MsG0580027605.01.T01:CDS
TTGATGATTTATTGGCATTA+GGG 0.250324 5:-64124124 None:intergenic
GTAAATACCCTTGGAGTTAT+AGG 0.279725 5:+64124101 MsG0580027605.01.T01:CDS
ACAAAATTGGAGGCATCATT+TGG 0.281644 5:+64124498 MsG0580027605.01.T01:CDS
ATTGATGATTTATTGGCATT+AGG 0.294469 5:-64124125 None:intergenic
TTGGACCAATTCTTCCATAT+TGG 0.298934 5:-64123977 None:intergenic
TCTTTAGAGCATACTTGATT+TGG 0.301730 5:-64123996 None:intergenic
AATTCTTCCATATTGGAGTT+TGG 0.331399 5:-64123970 None:intergenic
CACAAAATTACCAAAATAAC+AGG 0.354358 5:-64124437 None:intergenic
AAACTCCAATATGGAAGAAT+TGG 0.357083 5:+64123972 MsG0580027605.01.T01:CDS
TATCGTTCTGGATGTCTTGA+TGG 0.362209 5:+64124233 MsG0580027605.01.T01:CDS
TGGTGGAAGGGCTCTTGCTC+AGG 0.371596 5:+64124541 MsG0580027605.01.T01:CDS
AGCCAAACACATTATCGTTC+TGG 0.371977 5:+64124221 MsG0580027605.01.T01:CDS
GCTCTTGCTCAGGTTGTGTT+TGG 0.377174 5:+64124551 MsG0580027605.01.T01:CDS
ATCATTTGGGCTGGTTATCC+TGG 0.383440 5:+64124512 MsG0580027605.01.T01:CDS
AATCCAACCAAACTCCAATA+TGG 0.383933 5:+64123963 MsG0580027605.01.T01:CDS
GCTGGTTATCCTGGTGAACT+TGG 0.414067 5:+64124521 MsG0580027605.01.T01:CDS
ACTCCAAGGGTATTTACTCT+TGG 0.414446 5:-64124095 None:intergenic
ATAAGTTCCTGTTGCTTACC+TGG 0.420423 5:-64124386 None:intergenic
GTGTTTGGTGACTATAACCC+AGG 0.425278 5:+64124566 MsG0580027605.01.T01:CDS
ATAAGACTAGGCTTGTTTGA+TGG 0.425928 5:+64123939 None:intergenic
CAGCTTCAAGAGCAAGGTCT+AGG 0.436293 5:-64124022 None:intergenic
ATCCAGAACGATAATGTGTT+TGG 0.439275 5:-64124223 None:intergenic
CAAAATTGGAGGCATCATTT+GGG 0.439628 5:+64124499 MsG0580027605.01.T01:CDS
TAAGACTAGGCTTGTTTGAT+GGG 0.462964 5:+64123940 None:intergenic
TTAGCAAAAGTAATATCAAC+AGG 0.467973 5:-64124467 None:intergenic
CTTCCATATTGGAGTTTGGT+TGG 0.474146 5:-64123966 None:intergenic
AGAGTTACCAGGTAAGCAAC+AGG 0.474539 5:+64124379 MsG0580027605.01.T01:CDS
AGCATAGAAGAAAACTAACC+TGG 0.479258 5:-64124584 None:intergenic
AGGGTATTTACTCTTGGAAG+TGG 0.494590 5:-64124089 None:intergenic
GGTTATCCTGGTGAACTTGG+TGG 0.497246 5:+64124524 MsG0580027605.01.T01:CDS
ATCCTGGTGAACTTGGTGGA+AGG 0.508629 5:+64124528 MsG0580027605.01.T01:CDS
TTCTTGCAGCTTCAAGAGCA+AGG 0.526483 5:-64124028 None:intergenic
AAGAATAATGACAAAATTGG+AGG 0.530513 5:+64124488 MsG0580027605.01.T01:CDS
ATTGGAGGCATCATTTGGGC+TGG 0.536363 5:+64124503 MsG0580027605.01.T01:CDS
TCCTGGTGAACTTGGTGGAA+GGG 0.537602 5:+64124529 MsG0580027605.01.T01:CDS
TCTTGGAAGTGGGAGAATTG+AGG 0.537861 5:-64124078 None:intergenic
CTTCCAAGAGTAAATACCCT+TGG 0.550798 5:+64124092 MsG0580027605.01.T01:CDS
GCCCTTCCACCAAGTTCACC+AGG 0.555057 5:-64124530 None:intergenic
GCTCTTGAAGCTGCAAGAAG+TGG 0.571675 5:+64124032 MsG0580027605.01.T01:CDS
AGAGATCATTTAGAGTTACC+AGG 0.586212 5:+64124368 MsG0580027605.01.T01:CDS
CATTAGGGCCTATAACTCCA+AGG 0.594911 5:-64124109 None:intergenic
GGGTATTTACTCTTGGAAGT+GGG 0.605290 5:-64124088 None:intergenic
ATTGGATCAAAGTCAAGAGA+CGG 0.630240 5:+64124334 MsG0580027605.01.T01:CDS
GCATAGAAGAAAACTAACCT+GGG 0.643449 5:-64124583 None:intergenic
ATTAGGGCCTATAACTCCAA+GGG 0.662705 5:-64124108 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGTATCAATCTTAAA+AGG + Chr5:64124182-64124201 MsG0580027605.01.T01:CDS 15.0%
!!! AATAAGTGATTATGTTATTT+TGG + Chr5:64124304-64124323 MsG0580027605.01.T01:CDS 15.0%
!!! AAATCATCAATAGTTCTTTT+AGG + Chr5:64124137-64124156 MsG0580027605.01.T01:CDS 20.0%
!!! AAGAACTATTGATGATTTAT+TGG - Chr5:64124135-64124154 None:intergenic 20.0%
!!! AAGATTGATACATTTTTACA+AGG - Chr5:64124179-64124198 None:intergenic 20.0%
!!! GATTATGTTATTTTGGTTAT+GGG + Chr5:64124311-64124330 MsG0580027605.01.T01:CDS 20.0%
!!! TGATTATGTTATTTTGGTTA+TGG + Chr5:64124310-64124329 MsG0580027605.01.T01:CDS 20.0%
! AAGAATAATGACAAAATTGG+AGG + Chr5:64124488-64124507 MsG0580027605.01.T01:CDS 25.0%
! CACAAAATTACCAAAATAAC+AGG - Chr5:64124440-64124459 None:intergenic 25.0%
! GCTAAGAATAATGACAAAAT+TGG + Chr5:64124485-64124504 MsG0580027605.01.T01:CDS 25.0%
! TTAGCAAAAGTAATATCAAC+AGG - Chr5:64124470-64124489 None:intergenic 25.0%
!! ATTGATACATTTTTACAAGG+AGG - Chr5:64124176-64124195 None:intergenic 25.0%
!! ATTGATGATTTATTGGCATT+AGG - Chr5:64124128-64124147 None:intergenic 25.0%
!! TTGATGATTTATTGGCATTA+GGG - Chr5:64124127-64124146 None:intergenic 25.0%
!!! GTTATTTTGGTAATTTTGTG+TGG + Chr5:64124440-64124459 MsG0580027605.01.T01:CDS 25.0%
!!! GTTCTTTTAGGAAACTATAT+TGG + Chr5:64124149-64124168 MsG0580027605.01.T01:CDS 25.0%
AAACTCCAATATGGAAGAAT+TGG + Chr5:64123972-64123991 MsG0580027605.01.T01:CDS 30.0%
AATTCTTCCATATTGGAGTT+TGG - Chr5:64123973-64123992 None:intergenic 30.0%
!! ATTTTGGTAATTTTGTGTGG+TGG + Chr5:64124443-64124462 MsG0580027605.01.T01:CDS 30.0%
!! TCTTTAGAGCATACTTGATT+TGG - Chr5:64123999-64124018 None:intergenic 30.0%
!! TTCTAAGAAGCCTGTTATTT+TGG + Chr5:64124427-64124446 MsG0580027605.01.T01:CDS 30.0%
!!! TGTTATTTTGGTTATGGGAT+TGG + Chr5:64124316-64124335 MsG0580027605.01.T01:CDS 30.0%
AATCCAACCAAACTCCAATA+TGG + Chr5:64123963-64123982 MsG0580027605.01.T01:CDS 35.0%
ATCCAGAACGATAATGTGTT+TGG - Chr5:64124226-64124245 None:intergenic 35.0%
ATTGGATCAAAGTCAAGAGA+CGG + Chr5:64124334-64124353 MsG0580027605.01.T01:CDS 35.0%
GTAAATACCCTTGGAGTTAT+AGG + Chr5:64124101-64124120 MsG0580027605.01.T01:CDS 35.0%
TTGGACCAATTCTTCCATAT+TGG - Chr5:64123980-64123999 None:intergenic 35.0%
! AGAGATCATTTAGAGTTACC+AGG + Chr5:64124368-64124387 MsG0580027605.01.T01:CDS 35.0%
! TTTTAGCAACTTCAACTGCT+TGG - Chr5:64124286-64124305 None:intergenic 35.0%
!! ACAAAATTGGAGGCATCATT+TGG + Chr5:64124498-64124517 MsG0580027605.01.T01:CDS 35.0%
!! CAAAATTGGAGGCATCATTT+GGG + Chr5:64124499-64124518 MsG0580027605.01.T01:CDS 35.0%
AGCCAAACACATTATCGTTC+TGG + Chr5:64124221-64124240 MsG0580027605.01.T01:CDS 40.0%
ATTAGGGCCTATAACTCCAA+GGG - Chr5:64124111-64124130 None:intergenic 40.0%
CTTCCAAGAGTAAATACCCT+TGG + Chr5:64124092-64124111 MsG0580027605.01.T01:CDS 40.0%
CTTCCATATTGGAGTTTGGT+TGG - Chr5:64123969-64123988 None:intergenic 40.0%
GGGTATTTACTCTTGGAAGT+GGG - Chr5:64124091-64124110 None:intergenic 40.0%
! AGGGTATTTACTCTTGGAAG+TGG - Chr5:64124092-64124111 None:intergenic 40.0%
! ATAAGTTCCTGTTGCTTACC+TGG - Chr5:64124389-64124408 None:intergenic 40.0%
! TATCGTTCTGGATGTCTTGA+TGG + Chr5:64124233-64124252 MsG0580027605.01.T01:CDS 40.0%
!! ACTCCAAGGGTATTTACTCT+TGG - Chr5:64124098-64124117 None:intergenic 40.0%
AGAGTTACCAGGTAAGCAAC+AGG + Chr5:64124379-64124398 MsG0580027605.01.T01:CDS 45.0%
CATTAGGGCCTATAACTCCA+AGG - Chr5:64124112-64124131 None:intergenic 45.0%
TCTTGGAAGTGGGAGAATTG+AGG - Chr5:64124081-64124100 None:intergenic 45.0%
TTCTTGCAGCTTCAAGAGCA+AGG - Chr5:64124031-64124050 None:intergenic 45.0%
! GTGTTTGGTGACTATAACCC+AGG + Chr5:64124566-64124585 MsG0580027605.01.T01:CDS 45.0%
!! ATCATTTGGGCTGGTTATCC+TGG + Chr5:64124512-64124531 MsG0580027605.01.T01:CDS 45.0%
CAGCTTCAAGAGCAAGGTCT+AGG - Chr5:64124025-64124044 None:intergenic 50.0%
GCTCTTGAAGCTGCAAGAAG+TGG + Chr5:64124032-64124051 MsG0580027605.01.T01:CDS 50.0%
GCTGGTTATCCTGGTGAACT+TGG + Chr5:64124521-64124540 MsG0580027605.01.T01:CDS 50.0%
GGTTATCCTGGTGAACTTGG+TGG + Chr5:64124524-64124543 MsG0580027605.01.T01:CDS 50.0%
! ATCCTGGTGAACTTGGTGGA+AGG + Chr5:64124528-64124547 MsG0580027605.01.T01:CDS 50.0%
! GCTCTTGCTCAGGTTGTGTT+TGG + Chr5:64124551-64124570 MsG0580027605.01.T01:CDS 50.0%
! TCCTGGTGAACTTGGTGGAA+GGG + Chr5:64124529-64124548 MsG0580027605.01.T01:CDS 50.0%
!! ATTGGAGGCATCATTTGGGC+TGG + Chr5:64124503-64124522 MsG0580027605.01.T01:CDS 50.0%
GCCCTTCCACCAAGTTCACC+AGG - Chr5:64124533-64124552 None:intergenic 60.0%
TGGTGGAAGGGCTCTTGCTC+AGG + Chr5:64124541-64124560 MsG0580027605.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 64123951 64124592 64123951 ID=MsG0580027605.01;Name=MsG0580027605.01
Chr5 mRNA 64123951 64124592 64123951 ID=MsG0580027605.01.T01;Parent=MsG0580027605.01;Name=MsG0580027605.01.T01;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|213
Chr5 exon 64123951 64124592 64123951 ID=MsG0580027605.01.T01:exon:4510;Parent=MsG0580027605.01.T01
Chr5 CDS 64123951 64124592 64123951 ID=MsG0580027605.01.T01:cds;Parent=MsG0580027605.01.T01
Gene Sequence

>MsG0580027605.01.T01

TTGTTTGATGGGAATCCAACCAAACTCCAATATGGAAGAATTGGTCCAAATCAAGTATGCTCTAAAGAAAACCTAGACCTTGCTCTTGAAGCTGCAAGAAGTGGCATTGTACTTTTAAAAAACACTGCCTCAATTCTCCCACTTCCAAGAGTAAATACCCTTGGAGTTATAGGCCCTAATGCCAATAAATCATCAATAGTTCTTTTAGGAAACTATATTGGTCCTCCTTGTAAAAATGTATCAATCTTAAAAGGCTTTTACACTTATGCTAGCCAAACACATTATCGTTCTGGATGTCTTGATGGAACAAAGTGTGTTTCAGCTGAAATTGACCAAGCAGTTGAAGTTGCTAAAATAAGTGATTATGTTATTTTGGTTATGGGATTGGATCAAAGTCAAGAGACGGAAACACTTGACAGAGATCATTTAGAGTTACCAGGTAAGCAACAGGAACTTATTAAAAGTGTTGCTAAAGCTTCTAAGAAGCCTGTTATTTTGGTAATTTTGTGTGGTGGTCCTGTTGATATTACTTTTGCTAAGAATAATGACAAAATTGGAGGCATCATTTGGGCTGGTTATCCTGGTGAACTTGGTGGAAGGGCTCTTGCTCAGGTTGTGTTTGGTGACTATAACCCAGGTTAG

Protein sequence

>MsG0580027605.01.T01

LFDGNPTKLQYGRIGPNQVCSKENLDLALEAARSGIVLLKNTASILPLPRVNTLGVIGPNANKSSIVLLGNYIGPPCKNVSILKGFYTYASQTHYRSGCLDGTKCVSAEIDQAVEVAKISDYVILVMGLDQSQETETLDRDHLELPGKQQELIKSVAKASKKPVILVILCGGPVDITFAKNNDKIGGIIWAGYPGELGGRALAQVVFGDYNPG*