AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041441.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041441.01.T01 MTR_7g111120 98.990 99 1 0 1 99 319 417 2.44e-64 200
MsG0780041441.01.T01 MTR_1g103840 36.559 93 57 2 1 93 562 652 3.93e-14 66.6
MsG0780041441.01.T01 MTR_4g086490 32.609 92 60 2 2 93 925 1014 1.49e-12 62.4
MsG0780041441.01.T01 MTR_2g101710 33.721 86 55 2 2 87 401 484 2.03e-12 62.0
MsG0780041441.01.T01 MTR_8g086560 32.609 92 60 2 2 93 650 739 2.21e-12 61.6
MsG0780041441.01.T01 MTR_4g064770 40.000 95 55 2 2 96 457 549 3.21e-12 61.2
MsG0780041441.01.T01 MTR_7g083820 35.955 89 55 2 2 90 453 539 5.26e-12 60.8
MsG0780041441.01.T01 MTR_5g091640 34.831 89 55 3 1 89 416 501 7.93e-12 60.1
MsG0780041441.01.T01 MTR_2g436470 34.783 92 55 1 2 93 287 373 2.08e-11 58.9
MsG0780041441.01.T01 MTR_5g006570 35.065 77 48 2 1 77 618 692 2.69e-11 58.5
MsG0780041441.01.T01 MTR_5g027980 32.258 93 61 1 1 93 226 316 3.80e-11 58.2
MsG0780041441.01.T01 MTR_2g094430 29.348 92 63 2 2 93 459 548 5.14e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041441.01.T01 AT2G22070 39.785 93 54 2 1 93 566 656 5.03e-15 69.3
MsG0780041441.01.T01 AT5G08510 36.957 92 56 2 2 93 369 458 2.34e-13 64.7
MsG0780041441.01.T01 AT1G17630 42.105 76 42 2 2 77 587 660 1.31e-12 62.4
MsG0780041441.01.T01 AT1G17630 42.105 76 42 2 2 77 587 660 1.31e-12 62.4
MsG0780041441.01.T01 AT4G18750 33.696 92 59 2 2 93 652 741 6.06e-12 60.5
MsG0780041441.01.T01 AT1G09410 31.522 92 61 2 2 93 485 574 8.40e-12 60.1
MsG0780041441.01.T01 AT5G50990 38.043 92 52 2 2 93 318 404 1.12e-11 59.7
MsG0780041441.01.T01 AT5G59200 35.065 77 48 1 2 78 408 482 6.94e-11 57.4

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAGAAGTTGATTATTA+AGG 0.294543 7:-90942252 MsG0780041441.01.T01:CDS
GGAACCAGATGTTGTTGTTT+TGG 0.308448 7:-90942435 MsG0780041441.01.T01:CDS
TTCCTTCTTGTCAGCTTGCA+AGG 0.383847 7:-90942411 MsG0780041441.01.T01:CDS
TGACATACAAATCAGCAATA+AGG 0.392252 7:+90942303 None:intergenic
GCAAAGCCAGAAAATTATAG+AGG 0.398083 7:-90942335 MsG0780041441.01.T01:CDS
TATTAAGGAACATTTGAAAC+AGG 0.402540 7:-90942237 MsG0780041441.01.T01:CDS
ATTGCTGATTTGTATGTCAT+AGG 0.404345 7:-90942299 MsG0780041441.01.T01:CDS
TCTGTCGAAATTTAACCCCT+TGG 0.459067 7:+90942214 None:intergenic
GAACCCAAAACAACAACATC+TGG 0.475079 7:+90942431 None:intergenic
GGGTTAAATTTCGACAGAAA+CGG 0.480655 7:-90942209 MsG0780041441.01.T01:CDS
GATTATCAAAATGATGAAAG+TGG 0.512650 7:-90942456 MsG0780041441.01.T01:CDS
CTCCTTGCAAGCTGACAAGA+AGG 0.547042 7:+90942409 None:intergenic
GCAATTTGAGATTGCTGAGG+GGG 0.562458 7:-90942381 MsG0780041441.01.T01:CDS
GGAACATTTGAAACAGGCCA+AGG 0.567291 7:-90942231 MsG0780041441.01.T01:CDS
GAACATTTGAAACAGGCCAA+GGG 0.576485 7:-90942230 MsG0780041441.01.T01:CDS
GATTGAGCAGGTTCTGAGGA+TGG 0.580842 7:-90942357 MsG0780041441.01.T01:CDS
AAGCAATTTGAGATTGCTGA+GGG 0.589492 7:-90942383 MsG0780041441.01.T01:CDS
ATAAATCAGAAGAGGCTGAA+AGG 0.595466 7:-90942274 MsG0780041441.01.T01:CDS
TGGGAAAAGTGGGATGCTAG+AGG 0.604207 7:-90942489 None:intergenic
GGGTGATTGAGCAGGTTCTG+AGG 0.605555 7:-90942361 MsG0780041441.01.T01:CDS
CATAGGTGATAAATCAGAAG+AGG 0.606377 7:-90942282 MsG0780041441.01.T01:CDS
CAAAGCCAGAAAATTATAGA+GGG 0.615887 7:-90942334 MsG0780041441.01.T01:CDS
AGCAATTTGAGATTGCTGAG+GGG 0.621245 7:-90942382 MsG0780041441.01.T01:CDS
CAAGCAATTTGAGATTGCTG+AGG 0.621882 7:-90942384 MsG0780041441.01.T01:CDS
AACATTTGAAACAGGCCAAG+GGG 0.642858 7:-90942229 MsG0780041441.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTATCAAAATGATGAAAG+TGG - Chr7:90942219-90942238 MsG0780041441.01.T01:CDS 25.0%
!! GATGAAGAAGTTGATTATTA+AGG - Chr7:90942423-90942442 MsG0780041441.01.T01:CDS 25.0%
!! TATTAAGGAACATTTGAAAC+AGG - Chr7:90942438-90942457 MsG0780041441.01.T01:CDS 25.0%
CAAAGCCAGAAAATTATAGA+GGG - Chr7:90942341-90942360 MsG0780041441.01.T01:CDS 30.0%
TGACATACAAATCAGCAATA+AGG + Chr7:90942375-90942394 None:intergenic 30.0%
! TAAAGCCCTCTATAATTTTC+TGG + Chr7:90942349-90942368 None:intergenic 30.0%
!! ATTGCTGATTTGTATGTCAT+AGG - Chr7:90942376-90942395 MsG0780041441.01.T01:CDS 30.0%
ATAAATCAGAAGAGGCTGAA+AGG - Chr7:90942401-90942420 MsG0780041441.01.T01:CDS 35.0%
CATAGGTGATAAATCAGAAG+AGG - Chr7:90942393-90942412 MsG0780041441.01.T01:CDS 35.0%
GCAAAGCCAGAAAATTATAG+AGG - Chr7:90942340-90942359 MsG0780041441.01.T01:CDS 35.0%
GGGTTAAATTTCGACAGAAA+CGG - Chr7:90942466-90942485 MsG0780041441.01.T01:CDS 35.0%
! AAGCAATTTGAGATTGCTGA+GGG - Chr7:90942292-90942311 MsG0780041441.01.T01:CDS 35.0%
!!! GAACCAGATGTTGTTGTTTT+GGG - Chr7:90942241-90942260 MsG0780041441.01.T01:CDS 35.0%
GAACCCAAAACAACAACATC+TGG + Chr7:90942247-90942266 None:intergenic 40.0%
TCTGTCGAAATTTAACCCCT+TGG + Chr7:90942464-90942483 None:intergenic 40.0%
! AACATTTGAAACAGGCCAAG+GGG - Chr7:90942446-90942465 MsG0780041441.01.T01:CDS 40.0%
! AGCAATTTGAGATTGCTGAG+GGG - Chr7:90942293-90942312 MsG0780041441.01.T01:CDS 40.0%
! CAAGCAATTTGAGATTGCTG+AGG - Chr7:90942291-90942310 MsG0780041441.01.T01:CDS 40.0%
! GAACATTTGAAACAGGCCAA+GGG - Chr7:90942445-90942464 MsG0780041441.01.T01:CDS 40.0%
!!! GGAACCAGATGTTGTTGTTT+TGG - Chr7:90942240-90942259 MsG0780041441.01.T01:CDS 40.0%
TTCCTTCTTGTCAGCTTGCA+AGG - Chr7:90942264-90942283 MsG0780041441.01.T01:CDS 45.0%
! GCAATTTGAGATTGCTGAGG+GGG - Chr7:90942294-90942313 MsG0780041441.01.T01:CDS 45.0%
! GGAACATTTGAAACAGGCCA+AGG - Chr7:90942444-90942463 MsG0780041441.01.T01:CDS 45.0%
CTCCTTGCAAGCTGACAAGA+AGG + Chr7:90942269-90942288 None:intergenic 50.0%
!! GATTGAGCAGGTTCTGAGGA+TGG - Chr7:90942318-90942337 MsG0780041441.01.T01:CDS 50.0%
GGGTGATTGAGCAGGTTCTG+AGG - Chr7:90942314-90942333 MsG0780041441.01.T01:CDS 55.0%
TGCTGAGGGGGTGATTGAGC+AGG - Chr7:90942306-90942325 MsG0780041441.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 90942199 90942498 90942199 ID=MsG0780041441.01;Name=MsG0780041441.01
Chr7 mRNA 90942199 90942498 90942199 ID=MsG0780041441.01.T01;Parent=MsG0780041441.01;Name=MsG0780041441.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|99
Chr7 exon 90942199 90942498 90942199 ID=MsG0780041441.01.T01:exon:3901;Parent=MsG0780041441.01.T01
Chr7 CDS 90942199 90942498 90942199 ID=MsG0780041441.01.T01:cds;Parent=MsG0780041441.01.T01
Gene Sequence

>MsG0780041441.01.T01

ATGCTAGAGGAAGCTTATGAGATTATCAAAATGATGAAAGTGGAACCAGATGTTGTTGTTTTGGGTTCCTTCTTGTCAGCTTGCAAGGAGCACAAGCAATTTGAGATTGCTGAGGGGGTGATTGAGCAGGTTCTGAGGATGGCAAAGCCAGAAAATTATAGAGGGCTTTATAGCCTTATTGCTGATTTGTATGTCATAGGTGATAAATCAGAAGAGGCTGAAAGGATGAAGAAGTTGATTATTAAGGAACATTTGAAACAGGCCAAGGGGTTAAATTTCGACAGAAACGGATTAAGATAA

Protein sequence

>MsG0780041441.01.T01

MLEEAYEIIKMMKVEPDVVVLGSFLSACKEHKQFEIAEGVIEQVLRMAKPENYRGLYSLIADLYVIGDKSEEAERMKKLIIKEHLKQAKGLNFDRNGLR*