AlfalfaGEDB | MsG0680035464.01

M. sativa cultivar ZhongmuNo.1 / MsG0680035464.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035464.01.T01	XP_003620441.1	95.918	245	7	1	1	245	1	242	1.45E-165	467

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035464.01.T01	G7KN00	95.918	245	7	1	1	245	1	242	6.94e-166	467

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048633.01	MsG0680035464.01	0.803781	2.959742e-49	1.500495e-46
MsG0080048652.01	MsG0680035464.01	0.818057	2.390715e-52	1.770794e-49
MsG0180000367.01	MsG0680035464.01	0.820400	6.999690e-53	5.532894e-50
MsG0180001079.01	MsG0680035464.01	0.805454	1.324475e-49	7.010192e-47
MsG0180002808.01	MsG0680035464.01	0.849691	2.747762e-60	5.252440e-57
MsG0180003375.01	MsG0680035464.01	0.821525	3.854608e-53	3.143458e-50
MsG0180004705.01	MsG0680035464.01	0.802247	6.142623e-49	2.994406e-46
MsG0180005353.01	MsG0680035464.01	0.800286	1.547754e-48	7.179151e-46
MsG0180005369.01	MsG0680035464.01	-0.801197	1.008893e-48	4.788856e-46
MsG0180005757.01	MsG0680035464.01	0.823866	1.100004e-53	9.586914e-51
MsG0280006316.01	MsG0680035464.01	0.804318	2.288305e-49	1.176198e-46
MsG0280010617.01	MsG0680035464.01	0.840586	7.952532e-58	1.137968e-54
MsG0280011016.01	MsG0680035464.01	0.827270	1.716682e-54	1.649276e-51
MsG0380013041.01	MsG0680035464.01	0.800722	1.261144e-48	5.914578e-46
MsG0380013362.01	MsG0680035464.01	0.807230	5.593988e-50	3.099012e-47
MsG0380013415.01	MsG0680035464.01	0.822017	2.966006e-53	2.452736e-50
MsG0380014231.01	MsG0680035464.01	-0.803201	3.902291e-49	1.949093e-46
MsG0380014734.01	MsG0680035464.01	0.801349	9.390293e-49	4.474429e-46
MsG0380014735.01	MsG0680035464.01	-0.806569	7.717922e-50	4.203150e-47
MsG0680031464.01	MsG0680035464.01	0.810124	1.347184e-50	8.053985e-48
MsG0680033467.01	MsG0680035464.01	0.818188	2.233594e-52	1.660432e-49
MsG0680035464.01	MsG0780038307.01	0.812193	4.795714e-51	3.029228e-48
MsG0680035464.01	MsG0780038810.01	0.818272	2.136939e-52	1.592194e-49
MsG0680035464.01	MsG0780039916.01	0.807047	6.115555e-50	3.371829e-47
MsG0680035464.01	MsG0780039923.01	0.807235	5.581403e-50	3.092391e-47
MsG0680035464.01	MsG0780040034.01	0.803914	2.776802e-49	1.412690e-46
MsG0680035464.01	MsG0780040977.01	0.821805	3.320301e-53	2.729471e-50
MsG0680035464.01	MsG0780040991.01	0.802462	5.546000e-49	2.718398e-46
MsG0680035464.01	MsG0780041100.01	0.827804	1.277554e-54	1.246459e-51
MsG0680035464.01	MsG0780041180.01	0.831303	1.801457e-55	1.947021e-52
MsG0680035464.01	MsG0880042122.01	0.827354	1.638310e-54	1.577989e-51
MsG0680035464.01	MsG0880042635.01	0.801143	1.034743e-48	4.905047e-46
MsG0680035464.01	MsG0880043550.01	0.842080	3.216679e-58	4.822543e-55
MsG0680035464.01	MsG0880044545.01	-0.806659	7.388149e-50	4.032555e-47
MsG0680035464.01	MsG0880044688.01	0.823388	1.423097e-53	1.223466e-50
MsG0680035464.01	MsG0880045703.01	-0.815834	7.546822e-52	5.259678e-49
MsG0680035464.01	MsG0880045802.01	0.806205	9.209632e-50	4.969451e-47
MsG0680035464.01	MsG0880045856.01	0.803969	2.704196e-49	1.377650e-46
MsG0680035464.01	MsG0880045991.01	0.801786	7.640363e-49	3.680984e-46
MsG0680035464.01	MsG0880046402.01	0.821335	4.265620e-53	3.460393e-50
MsG0680035464.01	MsG0880046932.01	0.813083	3.062923e-51	1.980885e-48
MsG0680035464.01	MsG0880047450.01	0.806239	9.059077e-50	4.892371e-47
MsG0680035464.01	MsG0880047664.01	0.811661	6.262135e-51	3.898944e-48
MsG0680035464.01	MsG0880047714.01	0.820970	5.176917e-53	4.157632e-50
MsG0480020722.01	MsG0680035464.01	0.805481	1.307654e-49	6.925802e-47
MsG0480020814.01	MsG0680035464.01	0.813095	3.044643e-51	1.969658e-48
MsG0480021129.01	MsG0680035464.01	0.806413	8.324831e-50	4.515559e-47
MsG0480021433.01	MsG0680035464.01	0.807118	5.909616e-50	3.264145e-47
MsG0480022242.01	MsG0680035464.01	-0.815698	8.092853e-52	5.619558e-49
MsG0480022244.01	MsG0680035464.01	-0.814755	1.310780e-51	8.870542e-49
MsG0480022428.01	MsG0680035464.01	0.802953	4.392546e-49	2.180037e-46
MsG0480023322.01	MsG0680035464.01	0.825331	4.969664e-54	4.516150e-51
MsG0480023429.01	MsG0680035464.01	0.801731	7.839748e-49	3.771699e-46
MsG0480023851.01	MsG0680035464.01	-0.805994	1.020085e-49	5.474693e-47
MsG0580025023.01	MsG0680035464.01	0.839359	1.660827e-57	2.288144e-54
MsG0580025117.01	MsG0680035464.01	0.813481	2.504689e-51	1.637647e-48
MsG0580025486.01	MsG0680035464.01	-0.808644	2.799272e-50	1.609101e-47
MsG0380015828.01	MsG0680035464.01	-0.809070	2.269569e-50	1.319492e-47
MsG0380016819.01	MsG0680035464.01	0.803747	3.007103e-49	1.523197e-46
MsG0480018362.01	MsG0680035464.01	0.818784	1.635833e-52	1.236233e-49
MsG0480018439.01	MsG0680035464.01	0.802607	5.176472e-49	2.546626e-46
MsG0580026162.01	MsG0680035464.01	0.818827	1.599341e-52	1.210112e-49
MsG0580027276.01	MsG0680035464.01	-0.803684	3.099572e-49	1.567453e-46
MsG0580028302.01	MsG0680035464.01	0.825462	4.627084e-54	4.220087e-51
MsG0580028565.01	MsG0680035464.01	0.817730	2.833949e-52	2.080472e-49
MsG0580029751.01	MsG0680035464.01	-0.821104	4.821534e-53	3.886467e-50
MsG0680030635.01	MsG0680035464.01	-0.800070	1.712311e-48	7.898971e-46
MsG0280007163.01	MsG0680035464.01	0.807161	5.785039e-50	3.199045e-47
MsG0280007786.01	MsG0680035464.01	0.818300	2.106543e-52	1.570810e-49
MsG0280008226.01	MsG0680035464.01	0.833315	5.724308e-56	6.568297e-53
MsG0280009742.01	MsG0680035464.01	-0.801432	9.028246e-49	4.310944e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035464.01.T01	MTR_6g083930	95.918	245	7	1	1	245	1	242	1.76e-169	467
MsG0680035464.01.T01	MTR_8g104290	51.029	243	96	7	1	242	1	221	1.02e-53	173
MsG0680035464.01.T01	MTR_1g110410	38.433	268	117	8	1	235	5	257	1.43e-28	109
MsG0680035464.01.T01	MTR_6g018690	36.232	276	120	12	1	245	1	251	4.59e-24	97.1
MsG0680035464.01.T01	MTR_7g082180	37.603	242	117	12	1	217	5	237	3.79e-15	73.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035464.01.T01	AT1G48780	34.177	237	124	7	1	218	2	225	9.96e-21	88.2
MsG0680035464.01.T01	AT3G18300	32.168	286	139	10	1	244	2	274	2.23e-19	85.1
MsG0680035464.01.T01	AT3G18300	32.168	286	139	10	1	244	2	274	2.23e-19	85.1
MsG0680035464.01.T01	AT1G48780	41.346	104	58	1	115	218	55	155	8.04e-12	62.8
MsG0680035464.01.T01	AT1G67050	35.075	268	125	10	9	245	14	263	1.45e-11	63.2

Find 32 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTCTAACGGTTTCTGTTC+CGG	0.244916	6:+106209184	None:intergenic
AGGGAAGTGCAAATTTCTTT+AGG	0.276717	6:-106208827	MsG0680035464.01.T01:CDS
AAGAGTTATGGAGGATCTTA+TGG	0.313936	6:-106208903	MsG0680035464.01.T01:CDS
TTCACAAAAGGGCTGTGTTT+TGG	0.323412	6:+106209299	None:intergenic
TCTCTAACGGTTTCTGTTCC+GGG	0.327197	6:+106209185	None:intergenic
GATCTTATGGAAATGGTCAT+TGG	0.382081	6:-106208890	MsG0680035464.01.T01:CDS
AGTGCAAATTTCTTTAGGTT+TGG	0.385049	6:-106208822	MsG0680035464.01.T01:CDS
GTATGATTAGATTTCACAAA+AGG	0.414724	6:+106209287	None:intergenic
TCTAATTCAGGTAAGAGTTA+TGG	0.433779	6:-106208915	MsG0680035464.01.T01:CDS
TATGGAGGATCTTATGGAAA+TGG	0.449951	6:-106208897	MsG0680035464.01.T01:CDS
GGAGTTGGTACATTTAGAAC+AGG	0.467025	6:+106208861	None:intergenic
TTGTATAGAAGTAACTCAAC+TGG	0.473513	6:-106209041	MsG0680035464.01.T01:CDS
AAGTAATCCGAGGTGGATCT+TGG	0.474132	6:-106209213	MsG0680035464.01.T01:CDS
TATGATTAGATTTCACAAAA+GGG	0.504885	6:+106209288	None:intergenic
TAGAAGTAACTCAACTGGTT+CGG	0.505731	6:-106209036	MsG0680035464.01.T01:CDS
ACCAACTCCTTGTGTTTCAA+AGG	0.506237	6:-106208847	MsG0680035464.01.T01:CDS
AAAATTTCCTCCGCGTCCAT+CGG	0.507770	6:-106209264	MsG0680035464.01.T01:CDS
CCCTTTGAAACACAAGGAGT+TGG	0.518874	6:+106208846	None:intergenic
TTGTCAACATTTGAAGCCGA+TGG	0.519230	6:+106209248	None:intergenic
CCAACTCCTTGTGTTTCAAA+GGG	0.523892	6:-106208846	MsG0680035464.01.T01:CDS
AGGTGGATCTTGGCTCAGCC+CGG	0.535116	6:-106209203	MsG0680035464.01.T01:CDS
ACATTTGAAGCCGATGGACG+CGG	0.546498	6:+106209254	None:intergenic
GGCTGAGCCAAGATCCACCT+CGG	0.602931	6:+106209206	None:intergenic
GCACTTCCCTTTGAAACACA+AGG	0.612790	6:+106208840	None:intergenic
AATTCAGGTAAGAGTTATGG+AGG	0.623451	6:-106208912	MsG0680035464.01.T01:CDS
GTTGAGTTACTTCTATACAA+AGG	0.624764	6:+106209044	None:intergenic
GAGTTACTTCTATACAAAGG+AGG	0.663470	6:+106209047	None:intergenic
AGAAAGAAAGTAATCCGAGG+TGG	0.667008	6:-106209220	MsG0680035464.01.T01:CDS
TTTGAAGCCGATGGACGCGG+AGG	0.682178	6:+106209257	None:intergenic
TGAGAGAAAGAGAGACGCGG+CGG	0.683763	6:+106209473	None:intergenic
TGAAGAAAGAAAGTAATCCG+AGG	0.693640	6:-106209223	MsG0680035464.01.T01:CDS
TTATGAGAGAAAGAGAGACG+CGG	0.761843	6:+106209470	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGATTAGATTTCACAAAA+GGG	+	Chr6:106208988-106209007	None:intergenic	20.0%
!!!	TTCATTTTTAAGTATTGGAA+AGG	-	Chr6:106209474-106209493	MsG0680035464.01.T01:CDS	20.0%
!!!	TTTGGTTCATTTTTAAGTAT+TGG	-	Chr6:106209469-106209488	MsG0680035464.01.T01:CDS	20.0%
!	GTATGATTAGATTTCACAAA+AGG	+	Chr6:106208989-106209008	None:intergenic	25.0%
	CCTATGCAAAAATCTAATTC+AGG	-	Chr6:106209346-106209365	MsG0680035464.01.T01:CDS	30.0%
	CGCATTTAAAAACTCTCTAA+CGG	+	Chr6:106209104-106209123	None:intergenic	30.0%
	GTTGAGTTACTTCTATACAA+AGG	+	Chr6:106209232-106209251	None:intergenic	30.0%
	TCTAATTCAGGTAAGAGTTA+TGG	-	Chr6:106209358-106209377	MsG0680035464.01.T01:CDS	30.0%
	TTGTATAGAAGTAACTCAAC+TGG	-	Chr6:106209232-106209251	MsG0680035464.01.T01:CDS	30.0%
!	AGTGCAAATTTCTTTAGGTT+TGG	-	Chr6:106209451-106209470	MsG0680035464.01.T01:CDS	30.0%
!	CCTGAATTAGATTTTTGCAT+AGG	+	Chr6:106209349-106209368	None:intergenic	30.0%
	AAGAGTTATGGAGGATCTTA+TGG	-	Chr6:106209370-106209389	MsG0680035464.01.T01:CDS	35.0%
	AATTCAGGTAAGAGTTATGG+AGG	-	Chr6:106209361-106209380	MsG0680035464.01.T01:CDS	35.0%
	ACTAAATCCCCAAAAAGACT+TGG	+	Chr6:106209155-106209174	None:intergenic	35.0%
	GAGTTACTTCTATACAAAGG+AGG	+	Chr6:106209229-106209248	None:intergenic	35.0%
	GATCTTATGGAAATGGTCAT+TGG	-	Chr6:106209383-106209402	MsG0680035464.01.T01:CDS	35.0%
	TAGAAGTAACTCAACTGGTT+CGG	-	Chr6:106209237-106209256	MsG0680035464.01.T01:CDS	35.0%
	TATGGAGGATCTTATGGAAA+TGG	-	Chr6:106209376-106209395	MsG0680035464.01.T01:CDS	35.0%
	TGAAGAAAGAAAGTAATCCG+AGG	-	Chr6:106209050-106209069	MsG0680035464.01.T01:CDS	35.0%
!	AACACCATTCCAAGTCTTTT+TGG	-	Chr6:106209143-106209162	MsG0680035464.01.T01:CDS	35.0%
!	ACACCATTCCAAGTCTTTTT+GGG	-	Chr6:106209144-106209163	MsG0680035464.01.T01:CDS	35.0%
!	AGGGAAGTGCAAATTTCTTT+AGG	-	Chr6:106209446-106209465	MsG0680035464.01.T01:CDS	35.0%
!	GCTGATGAACTTTTCTCTAA+TGG	-	Chr6:106208941-106208960	MsG0680035464.01.T01:CDS	35.0%
	ACCAACTCCTTGTGTTTCAA+AGG	-	Chr6:106209426-106209445	MsG0680035464.01.T01:CDS	40.0%
	AGAAAGAAAGTAATCCGAGG+TGG	-	Chr6:106209053-106209072	MsG0680035464.01.T01:CDS	40.0%
	ATCCCCAAAAAGACTTGGAA+TGG	+	Chr6:106209150-106209169	None:intergenic	40.0%
	CCAACTCCTTGTGTTTCAAA+GGG	-	Chr6:106209427-106209446	MsG0680035464.01.T01:CDS	40.0%
	TTATGAGAGAAAGAGAGACG+CGG	+	Chr6:106208806-106208825	None:intergenic	40.0%
!	CACCATTCCAAGTCTTTTTG+GGG	-	Chr6:106209145-106209164	MsG0680035464.01.T01:CDS	40.0%
!	TTCACAAAAGGGCTGTGTTT+TGG	+	Chr6:106208977-106208996	None:intergenic	40.0%
!	TTGTCAACATTTGAAGCCGA+TGG	+	Chr6:106209028-106209047	None:intergenic	40.0%
!!	GGAGTTGGTACATTTAGAAC+AGG	+	Chr6:106209415-106209434	None:intergenic	40.0%
	AAAATTTCCTCCGCGTCCAT+CGG	-	Chr6:106209009-106209028	MsG0680035464.01.T01:CDS	45.0%
	AAGTAATCCGAGGTGGATCT+TGG	-	Chr6:106209060-106209079	MsG0680035464.01.T01:CDS	45.0%
	CTCTCTAACGGTTTCTGTTC+CGG	+	Chr6:106209092-106209111	None:intergenic	45.0%
	TCTCTAACGGTTTCTGTTCC+GGG	+	Chr6:106209091-106209110	None:intergenic	45.0%
!!	CAAAAGGGCTGTGTTTTGGA+AGG	+	Chr6:106208973-106208992	None:intergenic	45.0%
!!	CCCTTTGAAACACAAGGAGT+TGG	+	Chr6:106209430-106209449	None:intergenic	45.0%
!!	GCACTTCCCTTTGAAACACA+AGG	+	Chr6:106209436-106209455	None:intergenic	45.0%
!	ACATTTGAAGCCGATGGACG+CGG	+	Chr6:106209022-106209041	None:intergenic	50.0%
	TGAGAGAAAGAGAGACGCGG+CGG	+	Chr6:106208803-106208822	None:intergenic	55.0%
	AGGTGGATCTTGGCTCAGCC+CGG	-	Chr6:106209070-106209089	MsG0680035464.01.T01:CDS	60.0%
	GGCTGAGCCAAGATCCACCT+CGG	+	Chr6:106209070-106209089	None:intergenic	60.0%
!	TTTGAAGCCGATGGACGCGG+AGG	+	Chr6:106209019-106209038	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	106208779	106209516	106208779	ID=MsG0680035464.01;Name=MsG0680035464.01
Chr6	mRNA	106208779	106209516	106208779	ID=MsG0680035464.01.T01;Parent=MsG0680035464.01;Name=MsG0680035464.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|245
Chr6	exon	106208779	106209516	106208779	ID=MsG0680035464.01.T01:exon:11396;Parent=MsG0680035464.01.T01
Chr6	CDS	106208779	106209516	106208779	ID=MsG0680035464.01.T01:cds;Parent=MsG0680035464.01.T01

Gene Sequence

>MsG0680035464.01.T01

ATGTGCTCAGAAACAAGTACTCCGCCGCGTCTCTCTTTCTCTCATAATCTATCTGAGTTTCAACTTCAAAAAGATGTTACTTGCATAGAAACATTGTTGTTAGACTCAAATTCTGACTTTGAGTTCAACACAAGTAACATTCTTGAGTTTGAATCATCTTCAGCTGATGAACTTTTCTCTAATGGTAAGATCCTTCCAAAACACAGCCCTTTTGTGAAATCTAATCATACAAAATTTCCTCCGCGTCCATCGGCTTCAAATGTTGACAAAATGAAGAAAGAAAGTAATCCGAGGTGGATCTTGGCTCAGCCCGGAACAGAAACCGTTAGAGAGTTTTTAAATGCGAGTACTAATAACGAGAAAAAACACCATTCCAAGTCTTTTTGGGGATTTAGTAGAAGCAAAAGTCTCAATTGTGATACAAAGAAGAATTTGAGTTTTTCTTTACCTCCTTTGTATAGAAGTAACTCAACTGGTTCGGCGAAAAAAATGAGTTCAAACAAGCAGACATCATCAACAACAACAAAATCATCGTCGTCGTATTCATGCTCTAACATAAATTTGTATCCTATGCAAAAATCTAATTCAGGTAAGAGTTATGGAGGATCTTATGGAAATGGTCATTGGATTAGTCCTGTTCTAAATGTACCAACTCCTTGTGTTTCAAAGGGAAGTGCAAATTTCTTTAGGTTTGGTTCATTTTTAAGTATTGGAAAGGTTAAGAAGAGTAAGAAGTGA

Protein sequence

>MsG0680035464.01.T01

MCSETSTPPRLSFSHNLSEFQLQKDVTCIETLLLDSNSDFEFNTSNILEFESSSADELFSNGKILPKHSPFVKSNHTKFPPRPSASNVDKMKKESNPRWILAQPGTETVREFLNASTNNEKKHHSKSFWGFSRSKSLNCDTKKNLSFSLPPLYRSNSTGSAKKMSSNKQTSSTTTKSSSSYSCSNINLYPMQKSNSGKSYGGSYGNGHWISPVLNVPTPCVSKGSANFFRFGSFLSIGKVKKSKK*