AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0044210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0044210 MtrunA17_Chr1g0210821 94.444 54 3 0 1 54 1 54 2.31e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACTTGCTTGGTGTTCC+AGG 0.299965 1_1:+75703922 None:intergenic
TTCTGAGCTGGTGCAGCAGC+TGG 0.324083 1_1:+75703961 None:intergenic
TGTTGTTGATTCATCGGATT+CGG 0.387615 1_1:+75704018 None:intergenic
GAATGCTGTTGTTGATTCAT+CGG 0.467905 1_1:+75704012 None:intergenic
TGTGATTCTGTCAATCCCGC+TGG 0.500685 1_1:+75703847 None:intergenic
TGCATGATTGTGTTCCCAGC+GGG 0.524781 1_1:-75703862 Msa0044210:CDS
ACTTGCTTGGTGTTCCAGGT+TGG 0.540376 1_1:+75703926 None:intergenic
AAATAGTTGAGAAACTTGCT+TGG 0.585892 1_1:+75703913 None:intergenic
GTGATTCTGTCAATCCCGCT+GGG 0.586420 1_1:+75703848 None:intergenic
GGAGTTGCAGTTGATGAAGA+TGG 0.614276 1_1:+75703982 None:intergenic
GTGCATGATTGTGTTCCCAG+CGG 0.727375 1_1:-75703863 Msa0044210:CDS
GTTGCAGTTGATGAAGATGG+TGG 0.743712 1_1:+75703985 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATAGTTGAGAAACTTGCT+TGG + chr1_1:75704002-75704021 None:intergenic 30.0%
! ACTTTTTGGATTCAGAGAAG+AGG - chr1_1:75703869-75703888 Msa0044210:CDS 35.0%
!! TGTTGTTGATTCATCGGATT+CGG + chr1_1:75703897-75703916 None:intergenic 35.0%
!! GAATGCTGTTGTTGATTCAT+CGG + chr1_1:75703903-75703922 None:intergenic 35.0%
! TTTCTCTTTTTCTTCTGAGC+TGG + chr1_1:75703966-75703985 None:intergenic 35.0%
AAGAAAAAGAGAAACCAACC+TGG - chr1_1:75703972-75703991 Msa0044210:CDS 35.0%
!! TCTTCCTCAGCATCACTTTT+TGG - chr1_1:75703855-75703874 Msa0044210:CDS 40.0%
GAATCCAAAAAGTGATGCTG+AGG + chr1_1:75703862-75703881 None:intergenic 40.0%
!! GTTGCAGTTGATGAAGATGG+TGG + chr1_1:75703930-75703949 None:intergenic 45.0%
!! GGAGTTGCAGTTGATGAAGA+TGG + chr1_1:75703933-75703952 None:intergenic 45.0%
!! AGAAACTTGCTTGGTGTTCC+AGG + chr1_1:75703993-75704012 None:intergenic 45.0%
!! ACTTGCTTGGTGTTCCAGGT+TGG + chr1_1:75703989-75704008 None:intergenic 50.0%
GTGCATGATTGTGTTCCCAG+CGG - chr1_1:75704049-75704068 Msa0044210:CDS 50.0%
TGCATGATTGTGTTCCCAGC+GGG - chr1_1:75704050-75704069 Msa0044210:CDS 50.0%
GTGATTCTGTCAATCCCGCT+GGG + chr1_1:75704067-75704086 None:intergenic 50.0%
TGTGATTCTGTCAATCCCGC+TGG + chr1_1:75704068-75704087 None:intergenic 50.0%
TTCTGAGCTGGTGCAGCAGC+TGG + chr1_1:75703954-75703973 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 75703843 75704091 75703843 ID=Msa0044210;Name=Msa0044210
chr1_1 mRNA 75703843 75704091 75703843 ID=Msa0044210-mRNA-1;Parent=Msa0044210;Name=Msa0044210-mRNA-1;_AED=0.57;_eAED=0.57;_QI=0|-1|0|1|-1|1|1|0|82
chr1_1 exon 75703843 75704091 75703843 ID=Msa0044210-mRNA-1:exon:25653;Parent=Msa0044210-mRNA-1
chr1_1 CDS 75703843 75704091 75703843 ID=Msa0044210-mRNA-1:cds;Parent=Msa0044210-mRNA-1
Gene Sequence

>Msa0044210

ATGGCTGCATCATCTTCCTCAGCATCACTTTTTGGATTCAGAGAAGAGGATCCGAATCCGATGAATCAACAACAGCATTCTTTGCCACCATCTTCATCAACTGCAACTCCAGCTGCTGCACCAGCTCAGAAGAAAAAGAGAAACCAACCTGGAACACCAAGCAAGTTTCTCAACTATTTTATTGCAAATTTATGTTTAATCACTAAGTGCATGATTGTGTTCCCAGCGGGATTGACAGAATCACAGTGA

Protein sequence

>Msa0044210

MAASSSSASLFGFREEDPNPMNQQQHSLPPSSSTATPAAAPAQKKKRNQPGTPSKFLNYFIANLCLITKCMIVFPAGLTESQ*