Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0183600 | PNY10494.1 | 77.358 | 53 | 11 | 1 | 1 | 53 | 1 | 52 | 1.03e-15 | 75.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0183600 | A0A2K3P5D6 | 77.358 | 53 | 11 | 1 | 1 | 53 | 1 | 52 | 4.91e-16 | 75.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000490 | Msa0183600 | -0.817018 | 4.098070e-52 | -8.615850e-47 |
| Msa0044210 | Msa0183600 | 0.881293 | 2.706437e-70 | -8.615850e-47 |
| Msa0050430 | Msa0183600 | 0.804624 | 1.975869e-49 | -8.615850e-47 |
| Msa0095380 | Msa0183600 | 0.814691 | 1.353953e-51 | -8.615850e-47 |
| Msa0105420 | Msa0183600 | 0.818250 | 2.162038e-52 | -8.615850e-47 |
| Msa0183590 | Msa0183600 | 0.936449 | 1.706854e-97 | -8.615850e-47 |
| Msa0183600 | Msa0192060 | 0.810917 | 9.079137e-51 | -8.615850e-47 |
| Msa0183600 | Msa0224730 | 0.812007 | 5.265450e-51 | -8.615850e-47 |
| Msa0183600 | Msa0226890 | 0.807888 | 4.055944e-50 | -8.615850e-47 |
| Msa0183600 | Msa0228480 | 0.802287 | 6.025279e-49 | -8.615850e-47 |
| Msa0183600 | Msa0265520 | 0.803567 | 3.277138e-49 | -8.615850e-47 |
| Msa0183600 | Msa0270270 | 0.806136 | 9.522961e-50 | -8.615850e-47 |
| Msa0183600 | Msa0296760 | 0.800036 | 1.740115e-48 | -8.615850e-47 |
| Msa0183600 | Msa0320100 | 0.804581 | 2.017142e-49 | -8.615850e-47 |
| Msa0183600 | Msa0328210 | 0.808558 | 2.920015e-50 | -8.615850e-47 |
| Msa0183600 | Msa0344830 | 0.816030 | 6.821902e-52 | -8.615850e-47 |
| Msa0183600 | Msa0369570 | 0.801856 | 7.390230e-49 | -8.615850e-47 |
| Msa0183600 | Msa0376590 | 0.800300 | 1.537574e-48 | -8.615850e-47 |
| Msa0183600 | Msa0377530 | 0.816096 | 6.595803e-52 | -8.615850e-47 |
| Msa0183600 | Msa0380040 | 0.803696 | 3.082085e-49 | -8.615850e-47 |
| Msa0183600 | Msa0392070 | 0.813251 | 2.813230e-51 | -8.615850e-47 |
| Msa0183600 | Msa0398640 | 0.802896 | 4.513366e-49 | -8.615850e-47 |
| Msa0183600 | Msa0421780 | -0.805148 | 1.535437e-49 | -8.615850e-47 |
| Msa0183600 | Msa0442850 | 0.811886 | 5.592922e-51 | -8.615850e-47 |
| Msa0183600 | Msa0467900 | -0.811864 | 5.655174e-51 | -8.615850e-47 |
| Msa0183600 | Msa0467960 | -0.801914 | 7.192062e-49 | -8.615850e-47 |
| Msa0183600 | Msa0473240 | 0.813330 | 2.703919e-51 | -8.615850e-47 |
| Msa0183600 | Msa0475580 | 0.805135 | 1.545282e-49 | -8.615850e-47 |
| Msa0183600 | Msa0480320 | 0.833518 | 5.094085e-56 | -8.615850e-47 |
| Msa0183600 | Msa0489100 | 0.806359 | 8.548820e-50 | -8.615850e-47 |
| Msa0183600 | Msa0490350 | 0.803640 | 3.165015e-49 | -8.615850e-47 |
| Msa0183600 | Msa0505060 | 0.803138 | 4.022047e-49 | -8.615850e-47 |
| Msa0183600 | Msa0508200 | 0.804648 | 1.953284e-49 | -8.615850e-47 |
| Msa0183600 | Msa0516370 | 0.801677 | 8.044406e-49 | -8.615850e-47 |
| Msa0183600 | Msa0517490 | 0.825696 | 4.072397e-54 | -8.615850e-47 |
| Msa0183600 | Msa0519220 | 0.804414 | 2.185251e-49 | -8.615850e-47 |
| Msa0183600 | Msa0521880 | 0.819377 | 1.199167e-52 | -8.615850e-47 |
| Msa0183600 | Msa0535030 | 0.826806 | 2.215798e-54 | -8.615850e-47 |
| Msa0183600 | Msa0535060 | 0.828091 | 1.089832e-54 | -8.615850e-47 |
| Msa0183600 | Msa0591060 | 0.812491 | 4.128138e-51 | -8.615850e-47 |
| Msa0183600 | Msa0591770 | 0.829273 | 5.644212e-55 | -8.615850e-47 |
| Msa0183600 | Msa0618010 | 0.801244 | 9.865806e-49 | -8.615850e-47 |
| Msa0183600 | Msa0620810 | 0.803860 | 2.849710e-49 | -8.615850e-47 |
| Msa0183600 | Msa0638280 | 0.833781 | 4.379708e-56 | -8.615850e-47 |
| Msa0183600 | Msa0638320 | 0.836083 | 1.152076e-56 | -8.615850e-47 |
| Msa0183600 | Msa0669850 | 0.825127 | 5.552211e-54 | -8.615850e-47 |
| Msa0183600 | Msa0677270 | 0.825895 | 3.652514e-54 | -8.615850e-47 |
| Msa0183600 | Msa0689010 | 0.812656 | 3.799541e-51 | -8.615850e-47 |
| Msa0183600 | Msa0715110 | 0.813661 | 2.286559e-51 | -8.615850e-47 |
| Msa0183600 | Msa0743680 | 0.808941 | 2.418518e-50 | -8.615850e-47 |
| Msa0183600 | Msa0754720 | -0.814463 | 1.521024e-51 | -8.615850e-47 |
| Msa0183600 | Msa0756630 | -0.808669 | 2.765026e-50 | -8.615850e-47 |
| Msa0183600 | Msa0789570 | 0.803208 | 3.890944e-49 | -8.615850e-47 |
| Msa0183600 | Msa0855430 | 0.810123 | 1.348045e-50 | -8.615850e-47 |
| Msa0183600 | Msa0863800 | 0.804136 | 2.496696e-49 | -8.615850e-47 |
| Msa0183600 | Msa0881030 | 0.806707 | 7.217560e-50 | -8.615850e-47 |
| Msa0183600 | Msa0891040 | 0.802590 | 5.218652e-49 | -8.615850e-47 |
| Msa0183600 | Msa0893810 | 0.801392 | 9.200439e-49 | -8.615850e-47 |
| Msa0183600 | Msa0908050 | 0.815893 | 7.318367e-52 | -8.615850e-47 |
| Msa0183600 | Msa0933440 | 0.815411 | 9.373173e-52 | -8.615850e-47 |
| Msa0183600 | Msa0937670 | 0.811478 | 6.861857e-51 | -8.615850e-47 |
| Msa0183600 | Msa0969570 | 0.824391 | 8.279305e-54 | -8.615850e-47 |
| Msa0183600 | Msa0990960 | 0.806997 | 6.268322e-50 | -8.615850e-47 |
| Msa0183600 | Msa0993530 | 0.842003 | 3.370994e-58 | -8.615850e-47 |
| Msa0183600 | Msa1000940 | 0.820168 | 7.909827e-53 | -8.615850e-47 |
| Msa0183600 | Msa1026760 | 0.821678 | 3.553699e-53 | -8.615850e-47 |
| Msa0183600 | Msa1026790 | 0.819332 | 1.227598e-52 | -8.615850e-47 |
| Msa0183600 | Msa1037700 | -0.821946 | 3.081171e-53 | -8.615850e-47 |
| Msa0183600 | Msa1046300 | 0.813874 | 2.052623e-51 | -8.615850e-47 |
| Msa0183600 | Msa1084260 | 0.806672 | 7.343250e-50 | -8.615850e-47 |
| Msa0183600 | Msa1130210 | -0.828917 | 6.884473e-55 | -8.615850e-47 |
| Msa0183600 | Msa1185540 | 0.809601 | 1.745789e-50 | -8.615850e-47 |
| Msa0183600 | Msa1206020 | 0.810395 | 1.177769e-50 | -8.615850e-47 |
| Msa0183600 | Msa1217830 | 0.801730 | 7.842576e-49 | -8.615850e-47 |
| Msa0183600 | Msa1217860 | 0.820998 | 5.098777e-53 | -8.615850e-47 |
| Msa0183600 | Msa1239530 | 0.819916 | 9.033320e-53 | -8.615850e-47 |
| Msa0183600 | Msa1265070 | 0.826082 | 3.296553e-54 | -8.615850e-47 |
| Msa0183600 | Msa1271210 | 0.811660 | 6.263541e-51 | -8.615850e-47 |
| Msa0183600 | Msa1328850 | 0.804233 | 2.382853e-49 | -8.615850e-47 |
| Msa0183600 | Msa1362510 | 0.840351 | 9.165103e-58 | -8.615850e-47 |
| Msa0183600 | Msa1369530 | 0.819087 | 1.396035e-52 | -8.615850e-47 |
| Msa0183600 | Msa1412140 | 0.800314 | 1.527638e-48 | -8.615850e-47 |
| Msa0183600 | Msa1425250 | 0.815778 | 7.765029e-52 | -8.615850e-47 |
| Msa0183600 | Msa1439960 | 0.817078 | 3.972824e-52 | -8.615850e-47 |
| Msa0183600 | Msa1441190 | 0.821305 | 4.332442e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0183600 | MtrunA17_Chr1g0210821 | 96.226 | 53 | 2 | 0 | 1 | 53 | 1 | 53 | 2.26e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAACTTGCTTGGTGTTCC+AGG | 0.299965 | 1_4:+72550045 | None:intergenic |
| TTCTGAGCTGGTGCAGCAGC+TGG | 0.324083 | 1_4:+72550084 | None:intergenic |
| TGTTGTTGATTCATCGGATT+CGG | 0.387615 | 1_4:+72550141 | None:intergenic |
| GAATGCTGTTGTTGATTCAT+CGG | 0.467905 | 1_4:+72550135 | None:intergenic |
| ACTTGCTTGGTGTTCCAGGT+TGG | 0.540376 | 1_4:+72550049 | None:intergenic |
| GGTTTAGTGTATGTTTGTAT+CGG | 0.541346 | 1_4:+72549881 | None:intergenic |
| TTAGTGTATGTTTGTATCGG+TGG | 0.565691 | 1_4:+72549884 | None:intergenic |
| AAATAGTTGAGAAACTTGCT+TGG | 0.585892 | 1_4:+72550036 | None:intergenic |
| GGAGTTGCAGTTGATGAAGA+TGG | 0.614276 | 1_4:+72550105 | None:intergenic |
| GTTGCAGTTGATGAAGATGG+TGG | 0.743712 | 1_4:+72550108 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATAGTTGAGAAACTTGCT+TGG | + | chr1_4:72550043-72550062 | None:intergenic | 30.0% |
| ! | ACTTTTTGGATTCAGAGAAG+AGG | - | chr1_4:72549910-72549929 | Msa0183600:CDS | 35.0% |
| !! | TGTTGTTGATTCATCGGATT+CGG | + | chr1_4:72549938-72549957 | None:intergenic | 35.0% |
| !! | GAATGCTGTTGTTGATTCAT+CGG | + | chr1_4:72549944-72549963 | None:intergenic | 35.0% |
| ! | TTTCTCTTTTTCTTCTGAGC+TGG | + | chr1_4:72550007-72550026 | None:intergenic | 35.0% |
| AAGAAAAAGAGAAACCAACC+TGG | - | chr1_4:72550013-72550032 | Msa0183600:intron | 35.0% | |
| ! | AGTTTTTGTCACAATCAAGG+TGG | + | chr1_4:72550136-72550155 | None:intergenic | 35.0% |
| ! | CACAGTTTTTGTCACAATCA+AGG | + | chr1_4:72550139-72550158 | None:intergenic | 35.0% |
| TTAGTGTATGTTTGTATCGG+TGG | + | chr1_4:72550195-72550214 | None:intergenic | 35.0% | |
| !! | TCTTCCTCAGCATCACTTTT+TGG | - | chr1_4:72549896-72549915 | Msa0183600:CDS | 40.0% |
| GAATCCAAAAAGTGATGCTG+AGG | + | chr1_4:72549903-72549922 | None:intergenic | 40.0% | |
| !! | GTTGCAGTTGATGAAGATGG+TGG | + | chr1_4:72549971-72549990 | None:intergenic | 45.0% |
| !! | GGAGTTGCAGTTGATGAAGA+TGG | + | chr1_4:72549974-72549993 | None:intergenic | 45.0% |
| !! | AGAAACTTGCTTGGTGTTCC+AGG | + | chr1_4:72550034-72550053 | None:intergenic | 45.0% |
| TGCATGATTGTGTTCGCAGT+GGG | - | chr1_4:72550090-72550109 | Msa0183600:CDS | 45.0% | |
| !! | ACTTGCTTGGTGTTCCAGGT+TGG | + | chr1_4:72550030-72550049 | None:intergenic | 50.0% |
| GTGCATGATTGTGTTCGCAG+TGG | - | chr1_4:72550089-72550108 | Msa0183600:CDS | 50.0% | |
| TTCTGAGCTGGTGCAGCAGC+TGG | + | chr1_4:72549995-72550014 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 72549884 | 72550214 | 72549884 | ID=Msa0183600;Name=Msa0183600 |
| chr1_4 | mRNA | 72549884 | 72550214 | 72549884 | ID=Msa0183600-mRNA-1;Parent=Msa0183600;Name=Msa0183600-mRNA-1;_AED=0.68;_eAED=0.68;_QI=0|0|0|0.5|1|1|2|0|89 |
| chr1_4 | exon | 72550051 | 72550214 | 72550051 | ID=Msa0183600-mRNA-1:exon:24561;Parent=Msa0183600-mRNA-1 |
| chr1_4 | exon | 72549884 | 72549989 | 72549884 | ID=Msa0183600-mRNA-1:exon:24560;Parent=Msa0183600-mRNA-1 |
| chr1_4 | CDS | 72550051 | 72550214 | 72550051 | ID=Msa0183600-mRNA-1:cds;Parent=Msa0183600-mRNA-1 |
| chr1_4 | CDS | 72549884 | 72549989 | 72549884 | ID=Msa0183600-mRNA-1:cds;Parent=Msa0183600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
