AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0183590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0183590 MtrunA17_Chr1g0210821 96.226 53 2 0 1 53 1 53 2.26e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAACTTGCTTGGTGTTCC+AGG 0.299965 1_4:+72548472 None:intergenic
TTCTGAGCTGGTGCAGCAGC+TGG 0.324083 1_4:+72548511 None:intergenic
TGTTGTTGATTCATCGGATT+CGG 0.387615 1_4:+72548568 None:intergenic
GAATGCTGTTGTTGATTCAT+CGG 0.467905 1_4:+72548562 None:intergenic
ACTTGCTTGGTGTTCCAGGT+TGG 0.540376 1_4:+72548476 None:intergenic
GGTTTAGTGTATGTTTGTAT+CGG 0.541346 1_4:+72548307 None:intergenic
TTAGTGTATGTTTGTATCGG+TGG 0.565691 1_4:+72548310 None:intergenic
AAATAGTTGAGAAACTTGCT+TGG 0.585892 1_4:+72548463 None:intergenic
GGAGTTGCAGTTGATGAAGA+TGG 0.614276 1_4:+72548532 None:intergenic
GTTGCAGTTGATGAAGATGG+TGG 0.743712 1_4:+72548535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATAGTTGAGAAACTTGCT+TGG + chr1_4:72548469-72548488 None:intergenic 30.0%
! ACTTTTTGGATTCAGAGAAG+AGG - chr1_4:72548336-72548355 Msa0183590:CDS 35.0%
!! TGTTGTTGATTCATCGGATT+CGG + chr1_4:72548364-72548383 None:intergenic 35.0%
!! GAATGCTGTTGTTGATTCAT+CGG + chr1_4:72548370-72548389 None:intergenic 35.0%
! TTTCTCTTTTTCTTCTGAGC+TGG + chr1_4:72548433-72548452 None:intergenic 35.0%
AAGAAAAAGAGAAACCAACC+TGG - chr1_4:72548439-72548458 Msa0183590:intron 35.0%
! AGTTTTTGTCACAATCAAGG+TGG + chr1_4:72548563-72548582 None:intergenic 35.0%
! CACAGTTTTTGTCACAATCA+AGG + chr1_4:72548566-72548585 None:intergenic 35.0%
TTAGTGTATGTTTGTATCGG+TGG + chr1_4:72548622-72548641 None:intergenic 35.0%
!! TCTTCCTCAGCATCACTTTT+TGG - chr1_4:72548322-72548341 Msa0183590:CDS 40.0%
GAATCCAAAAAGTGATGCTG+AGG + chr1_4:72548329-72548348 None:intergenic 40.0%
!! GTTGCAGTTGATGAAGATGG+TGG + chr1_4:72548397-72548416 None:intergenic 45.0%
!! GGAGTTGCAGTTGATGAAGA+TGG + chr1_4:72548400-72548419 None:intergenic 45.0%
!! AGAAACTTGCTTGGTGTTCC+AGG + chr1_4:72548460-72548479 None:intergenic 45.0%
TGCATGATTGTGTTCGCAGT+GGG - chr1_4:72548517-72548536 Msa0183590:CDS 45.0%
!! ACTTGCTTGGTGTTCCAGGT+TGG + chr1_4:72548456-72548475 None:intergenic 50.0%
GTGCATGATTGTGTTCGCAG+TGG - chr1_4:72548516-72548535 Msa0183590:CDS 50.0%
TTCTGAGCTGGTGCAGCAGC+TGG + chr1_4:72548421-72548440 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 72548310 72548641 72548310 ID=Msa0183590;Name=Msa0183590
chr1_4 mRNA 72548310 72548641 72548310 ID=Msa0183590-mRNA-1;Parent=Msa0183590;Name=Msa0183590-mRNA-1;_AED=0.66;_eAED=0.66;_QI=0|0|0|0.5|1|1|2|0|89
chr1_4 exon 72548478 72548641 72548478 ID=Msa0183590-mRNA-1:exon:24559;Parent=Msa0183590-mRNA-1
chr1_4 exon 72548310 72548415 72548310 ID=Msa0183590-mRNA-1:exon:24558;Parent=Msa0183590-mRNA-1
chr1_4 CDS 72548478 72548641 72548478 ID=Msa0183590-mRNA-1:cds;Parent=Msa0183590-mRNA-1
chr1_4 CDS 72548310 72548415 72548310 ID=Msa0183590-mRNA-1:cds;Parent=Msa0183590-mRNA-1
Gene Sequence

>Msa0183590

ATGGCTGCATCATCTTCCTCAGCATCACTTTTTGGATTCAGAGAAGAGGATCCGAATCCGATGAATCAACAACAGCATTCTTTGCCACCATCTTCATCAACTGCAACTCCAGCTGCTGCACCAGCTCAGAAGAAAAAGAGAAACCAACCTGGAACACCAAGCAATGGGATTGACAGAATCACAGTGAACCACCTTGATTGTGACAAAAACTGTGCTTTGAAACTTCACTGTGATTCTGCTAAACTCACCACCGATACAAACATACACTAA

Protein sequence

>Msa0183590

MAASSSSASLFGFREEDPNPMNQQQHSLPPSSSTATPAAAPAQKKKRNQPGTPSNGIDRITVNHLDCDKNCALKLHCDSAKLTTDTNIH*