Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650730 | XP_003607784.1 | 91.358 | 81 | 7 | 0 | 1 | 81 | 1 | 81 | 9.78e-36 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000490 | Msa0650730 | -0.815450 | 9.187527e-52 | -8.615850e-47 |
| Msa0032610 | Msa0650730 | 0.810083 | 1.375195e-50 | -8.615850e-47 |
| Msa0609970 | Msa0650730 | 0.808167 | 3.538458e-50 | -8.615850e-47 |
| Msa0614990 | Msa0650730 | 0.874034 | 9.254919e-68 | -8.615850e-47 |
| Msa0650620 | Msa0650730 | 0.831485 | 1.625036e-55 | -8.615850e-47 |
| Msa0650640 | Msa0650730 | 0.804935 | 1.700793e-49 | -8.615850e-47 |
| Msa0650730 | Msa0650740 | 0.807590 | 4.693033e-50 | -8.615850e-47 |
| Msa0650730 | Msa0709670 | 0.819712 | 1.005429e-52 | -8.615850e-47 |
| Msa0650730 | Msa0755080 | 0.878384 | 2.933107e-69 | -8.615850e-47 |
| Msa0650730 | Msa0755090 | 0.803511 | 3.366092e-49 | -8.615850e-47 |
| Msa0650730 | Msa0796950 | 0.839147 | 1.885047e-57 | -8.615850e-47 |
| Msa0650730 | Msa0797000 | 0.814118 | 1.813209e-51 | -8.615850e-47 |
| Msa0650730 | Msa0834450 | 0.872910 | 2.212261e-67 | -8.615850e-47 |
| Msa0650730 | Msa0834460 | 0.835636 | 1.495080e-56 | -8.615850e-47 |
| Msa0650730 | Msa0834600 | 0.800714 | 1.266364e-48 | -8.615850e-47 |
| Msa0650730 | Msa0898860 | 0.804199 | 2.422559e-49 | -8.615850e-47 |
| Msa0650730 | Msa0928030 | 0.819589 | 1.072845e-52 | -8.615850e-47 |
| Msa0650730 | Msa1077670 | 0.819248 | 1.283412e-52 | -8.615850e-47 |
| Msa0650730 | Msa1174870 | 0.802912 | 4.478044e-49 | -8.615850e-47 |
| Msa0650730 | Msa1182640 | 0.823685 | 1.212731e-53 | -8.615850e-47 |
| Msa0650730 | Msa1207460 | -0.830509 | 2.821579e-55 | -8.615850e-47 |
| Msa0650730 | Msa1229850 | 0.820518 | 6.573389e-53 | -8.615850e-47 |
| Msa0650730 | Msa1234690 | 0.804628 | 1.971407e-49 | -8.615850e-47 |
| Msa0650730 | Msa1234730 | 0.806895 | 6.586486e-50 | -8.615850e-47 |
| Msa0650730 | Msa1272520 | 0.818027 | 2.428555e-52 | -8.615850e-47 |
| Msa0650730 | Msa1287360 | 0.807023 | 6.187978e-50 | -8.615850e-47 |
| Msa0650730 | Msa1315110 | 0.808222 | 3.443281e-50 | -8.615850e-47 |
| Msa0650730 | Msa1350690 | 0.823257 | 1.526622e-53 | -8.615850e-47 |
| Msa0650730 | Msa1360280 | 0.803892 | 2.806213e-49 | -8.615850e-47 |
| Msa0650730 | Msa1459760 | -0.821306 | 4.331758e-53 | -8.615850e-47 |
| Msa0650730 | Msa1465960 | 0.807264 | 5.502213e-50 | -8.615850e-47 |
| Msa0148120 | Msa0650730 | 0.808751 | 2.655414e-50 | -8.615850e-47 |
| Msa0197180 | Msa0650730 | 0.800618 | 1.324183e-48 | -8.615850e-47 |
| Msa0223100 | Msa0650730 | 0.808721 | 2.695274e-50 | -8.615850e-47 |
| Msa0223290 | Msa0650730 | 0.809583 | 1.761118e-50 | -8.615850e-47 |
| Msa0223380 | Msa0650730 | 0.818683 | 1.724643e-52 | -8.615850e-47 |
| Msa0379410 | Msa0650730 | 0.803214 | 3.878560e-49 | -8.615850e-47 |
| Msa0384540 | Msa0650730 | 0.805602 | 1.233261e-49 | -8.615850e-47 |
| Msa0404700 | Msa0650730 | 0.802663 | 5.041769e-49 | -8.615850e-47 |
| Msa0426810 | Msa0650730 | 0.812064 | 5.116702e-51 | -8.615850e-47 |
| Msa0462540 | Msa0650730 | 0.806197 | 9.247944e-50 | -8.615850e-47 |
| Msa0468130 | Msa0650730 | 0.806041 | 9.973887e-50 | -8.615850e-47 |
| Msa0479040 | Msa0650730 | 0.811196 | 7.901266e-51 | -8.615850e-47 |
| Msa0479090 | Msa0650730 | 0.807293 | 5.425255e-50 | -8.615850e-47 |
| Msa0266210 | Msa0650730 | 0.823363 | 1.442115e-53 | -8.615850e-47 |
| Msa0279210 | Msa0650730 | -0.833436 | 5.338620e-56 | -8.615850e-47 |
| Msa0287670 | Msa0650730 | 0.823335 | 1.463730e-53 | -8.615850e-47 |
| Msa0308770 | Msa0650730 | 0.805077 | 1.588452e-49 | -8.615850e-47 |
| Msa0318970 | Msa0650730 | -0.826790 | 2.235448e-54 | -8.615850e-47 |
| Msa0362060 | Msa0650730 | 0.838203 | 3.305891e-57 | -8.615850e-47 |
| Msa0363100 | Msa0650730 | 0.802166 | 6.381174e-49 | -8.615850e-47 |
| Msa0363170 | Msa0650730 | 0.806404 | 8.361108e-50 | -8.615850e-47 |
| Msa0579340 | Msa0650730 | 0.822824 | 1.926784e-53 | -8.615850e-47 |
| Msa0579350 | Msa0650730 | 0.892093 | 2.180034e-74 | -8.615850e-47 |
| Msa0579370 | Msa0650730 | 0.834617 | 2.702275e-56 | -8.615850e-47 |
| Msa0581920 | Msa0650730 | 0.818501 | 1.896353e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650730 | MtrunA17_Chr4g0042971 | 47.500 | 80 | 36 | 2 | 4 | 81 | 81 | 156 | 3.19e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGACCTAACGATATTT+TGG | 0.245207 | 4_3:+57991219 | Msa0650730:CDS |
| AAAACCAAAGGTGCGGTTAA+TGG | 0.283737 | 4_3:+57991406 | Msa0650730:CDS |
| TTTGCCAATAGGATCTGGTT+TGG | 0.364938 | 4_3:-57991386 | None:intergenic |
| TTGCCAATAGGATCTGGTTT+GGG | 0.376061 | 4_3:-57991385 | None:intergenic |
| AGCACCATTAACCGCACCTT+TGG | 0.426753 | 4_3:-57991410 | None:intergenic |
| GCACTTGCCAAAATATCGTT+AGG | 0.455143 | 4_3:-57991226 | None:intergenic |
| AAACCCAAACCAGATCCTAT+TGG | 0.473112 | 4_3:+57991382 | Msa0650730:CDS |
| TAAACAACAAAATCGATGGC+TGG | 0.494913 | 4_3:+57991202 | None:intergenic |
| GGTGCTACAAAGTCTGTTGG+AGG | 0.564564 | 4_3:+57991427 | Msa0650730:CDS |
| ACTGTAGGAAATGCTGCAGC+TGG | 0.565617 | 4_3:+57991277 | Msa0650730:CDS |
| GCTGGTGTTCATAAGACTGT+AGG | 0.573068 | 4_3:+57991328 | Msa0650730:CDS |
| AATGGTGCTACAAAGTCTGT+TGG | 0.575777 | 4_3:+57991424 | Msa0650730:CDS |
| ACTGTGGGAAATGCTGCAGC+TGG | 0.580044 | 4_3:+57991310 | Msa0650730:CDS |
| GCTTCAGCACAGAAAACTGT+AGG | 0.594928 | 4_3:+57991262 | Msa0650730:CDS |
| GATCCTATTGGCAAAACCAA+AGG | 0.604323 | 4_3:+57991394 | Msa0650730:CDS |
| AGCTGGTGCCCAGAAAACTG+TGG | 0.654418 | 4_3:+57991294 | Msa0650730:CDS |
| TGTAGGAGACTATGCTACCA+TGG | 0.655972 | 4_3:+57991345 | Msa0650730:CDS |
| TATTGGCAAAACCAAAGGTG+CGG | 0.681681 | 4_3:+57991399 | Msa0650730:CDS |
| GCTGGTGCCCAGAAAACTGT+GGG | 0.683027 | 4_3:+57991295 | Msa0650730:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTACATAATCAGCAGCCA+TGG | - | chr4_3:57991365-57991384 | None:intergenic | 35.0% |
| GCACTTGCCAAAATATCGTT+AGG | - | chr4_3:57991229-57991248 | None:intergenic | 40.0% | |
| !!! | CGATATTTTGGCAAGTGCAA+AGG | + | chr4_3:57991231-57991250 | Msa0650730:CDS | 40.0% |
| ! | AGCATTTCCCACAGTTTTCT+GGG | - | chr4_3:57991305-57991324 | None:intergenic | 40.0% |
| AAACCCAAACCAGATCCTAT+TGG | + | chr4_3:57991382-57991401 | Msa0650730:CDS | 40.0% | |
| ! | TTGCCAATAGGATCTGGTTT+GGG | - | chr4_3:57991388-57991407 | None:intergenic | 40.0% |
| TTTGCCAATAGGATCTGGTT+TGG | - | chr4_3:57991389-57991408 | None:intergenic | 40.0% | |
| !!! | TTGGTTTTGCCAATAGGATC+TGG | - | chr4_3:57991394-57991413 | None:intergenic | 40.0% |
| GATCCTATTGGCAAAACCAA+AGG | + | chr4_3:57991394-57991413 | Msa0650730:CDS | 40.0% | |
| TATTGGCAAAACCAAAGGTG+CGG | + | chr4_3:57991399-57991418 | Msa0650730:CDS | 40.0% | |
| ! | AAAACCAAAGGTGCGGTTAA+TGG | + | chr4_3:57991406-57991425 | Msa0650730:CDS | 40.0% |
| !! | AATGGTGCTACAAAGTCTGT+TGG | + | chr4_3:57991424-57991443 | Msa0650730:CDS | 40.0% |
| ! | GGCTGGACCTAACGATATTT+TGG | + | chr4_3:57991219-57991238 | Msa0650730:CDS | 45.0% |
| GCTTCAGCACAGAAAACTGT+AGG | + | chr4_3:57991262-57991281 | Msa0650730:CDS | 45.0% | |
| ! | CAGCATTTCCCACAGTTTTC+TGG | - | chr4_3:57991306-57991325 | None:intergenic | 45.0% |
| GCTGGTGTTCATAAGACTGT+AGG | + | chr4_3:57991328-57991347 | Msa0650730:CDS | 45.0% | |
| TGTAGGAGACTATGCTACCA+TGG | + | chr4_3:57991345-57991364 | Msa0650730:CDS | 45.0% | |
| !!! | GCACCTTTGGTTTTGCCAAT+AGG | - | chr4_3:57991400-57991419 | None:intergenic | 45.0% |
| ACTGTAGGAAATGCTGCAGC+TGG | + | chr4_3:57991277-57991296 | Msa0650730:CDS | 50.0% | |
| AGCACCATTAACCGCACCTT+TGG | - | chr4_3:57991413-57991432 | None:intergenic | 50.0% | |
| ! | GGTGCTACAAAGTCTGTTGG+AGG | + | chr4_3:57991427-57991446 | Msa0650730:CDS | 50.0% |
| AGCTGGTGCCCAGAAAACTG+TGG | + | chr4_3:57991294-57991313 | Msa0650730:CDS | 55.0% | |
| GCTGGTGCCCAGAAAACTGT+GGG | + | chr4_3:57991295-57991314 | Msa0650730:CDS | 55.0% | |
| ACTGTGGGAAATGCTGCAGC+TGG | + | chr4_3:57991310-57991329 | Msa0650730:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 57991217 | 57991462 | 57991217 | ID=Msa0650730;Name=Msa0650730 |
| chr4_3 | mRNA | 57991217 | 57991462 | 57991217 | ID=Msa0650730-mRNA-1;Parent=Msa0650730;Name=Msa0650730-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|81 |
| chr4_3 | exon | 57991217 | 57991462 | 57991217 | ID=Msa0650730-mRNA-1:exon:15271;Parent=Msa0650730-mRNA-1 |
| chr4_3 | CDS | 57991217 | 57991462 | 57991217 | ID=Msa0650730-mRNA-1:cds;Parent=Msa0650730-mRNA-1 |
| Gene Sequence |
| Protein sequence |
