AlfalfaGEDB | Msa0169410

M. sativa cultivar zhongmu-4 / Msa0169410

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0006091 GO:0006810 GO:0008150 GO:0008152 GO:0009506 GO:0009507 GO:0009536 GO:0009767 GO:0009773 GO:0009987 GO:0010496 GO:0010497 GO:0010598 GO:0015979 GO:0016020 GO:0016032 GO:0019684 GO:0022900 GO:0030054 GO:0032991 GO:0040011 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044000 GO:0044237 GO:0044403 GO:0044419 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0044766 GO:0046739 GO:0046794 GO:0051179 GO:0051234 GO:0051701 GO:0051704 GO:0051814 GO:0052126 GO:0052192 GO:0055044 GO:0055114 GO:0098796 GO:1902579
PFAM: PF01657.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0169410	AES60997.1	98.418	316	5	0	1	316	1	316	0.0	632

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0169410	sp\|Q6NM73\|PDLP2_ARATH	67.230	296	89	2	8	297	10	303	1.25e-134	387

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0169410	G7I3U3	98.418	316	5	0	1	316	1	316	0.0	632

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002420	Msa0169410	0.823349	1.453187e-53	-8.615850e-47
Msa0029380	Msa0169410	0.875422	3.119965e-68	-8.615850e-47
Msa0033220	Msa0169410	0.811616	6.405695e-51	-8.615850e-47
Msa0040790	Msa0169410	0.838921	2.157313e-57	-8.615850e-47
Msa0044160	Msa0169410	0.811708	6.115759e-51	-8.615850e-47
Msa0077340	Msa0169410	0.832744	7.935906e-56	-8.615850e-47
Msa0077560	Msa0169410	0.836666	8.184864e-57	-8.615850e-47
Msa0079880	Msa0169410	0.801322	9.510359e-49	-8.615850e-47
Msa0124910	Msa0169410	0.906759	1.052810e-80	-8.615850e-47
Msa0125360	Msa0169410	0.808165	3.541674e-50	-8.615850e-47
Msa0139500	Msa0169410	0.813041	3.128914e-51	-8.615850e-47
Msa0142430	Msa0169410	0.848065	7.771662e-60	-8.615850e-47
Msa0169410	Msa0183710	0.822608	2.162895e-53	-8.615850e-47
Msa0169410	Msa0184500	0.822351	2.481135e-53	-8.615850e-47
Msa0169410	Msa0189270	0.812545	4.017948e-51	-8.615850e-47
Msa0169410	Msa0195230	0.826037	3.379828e-54	-8.615850e-47
Msa0169410	Msa0195260	0.817696	2.883971e-52	-8.615850e-47
Msa0169410	Msa0203080	0.800575	1.351630e-48	-8.615850e-47
Msa0169410	Msa0204950	0.837481	5.067737e-57	-8.615850e-47
Msa0169410	Msa0209140	0.812811	3.513678e-51	-8.615850e-47
Msa0169410	Msa0225480	0.808701	2.721812e-50	-8.615850e-47
Msa0169410	Msa0249850	0.839010	2.046073e-57	-8.615850e-47
Msa0169410	Msa0269210	0.842682	2.227859e-58	-8.615850e-47
Msa0169410	Msa0276860	0.815656	8.265098e-52	-8.615850e-47
Msa0169410	Msa0281470	0.825314	5.015423e-54	-8.615850e-47
Msa0169410	Msa0288620	0.812383	4.358234e-51	-8.615850e-47
Msa0169410	Msa0307270	0.820047	8.431097e-53	-8.615850e-47
Msa0169410	Msa0309720	0.811092	8.320296e-51	-8.615850e-47
Msa0169410	Msa0320600	0.843618	1.254498e-58	-8.615850e-47
Msa0169410	Msa0326710	0.813504	2.474875e-51	-8.615850e-47
Msa0169410	Msa0330520	0.806692	7.271175e-50	-8.615850e-47
Msa0169410	Msa0354840	0.820053	8.402751e-53	-8.615850e-47
Msa0169410	Msa0361910	0.806039	9.981409e-50	-8.615850e-47
Msa0169410	Msa0389690	0.826096	3.271777e-54	-8.615850e-47
Msa0169410	Msa0412790	0.840449	8.637734e-58	-8.615850e-47
Msa0169410	Msa0414880	0.819746	9.878881e-53	-8.615850e-47
Msa0169410	Msa0416020	0.813567	2.397567e-51	-8.615850e-47
Msa0169410	Msa0430230	0.832478	9.238340e-56	-8.615850e-47
Msa0169410	Msa0437480	0.802683	4.993330e-49	-8.615850e-47
Msa0169410	Msa0437520	0.804545	2.052375e-49	-8.615850e-47
Msa0169410	Msa0440350	0.832076	1.161577e-55	-8.615850e-47
Msa0169410	Msa0454560	0.838376	2.983919e-57	-8.615850e-47
Msa0169410	Msa0481890	0.806734	7.125013e-50	-8.615850e-47
Msa0169410	Msa0500650	0.804697	1.907317e-49	-8.615850e-47
Msa0169410	Msa0502510	0.861593	9.198466e-64	-8.615850e-47
Msa0169410	Msa0508180	0.832651	8.369121e-56	-8.615850e-47
Msa0169410	Msa0519760	0.812221	4.727340e-51	-8.615850e-47
Msa0169410	Msa0519810	0.813435	2.563089e-51	-8.615850e-47
Msa0169410	Msa0519840	0.814010	1.915000e-51	-8.615850e-47
Msa0169410	Msa0525880	0.807347	5.285059e-50	-8.615850e-47
Msa0169410	Msa0545920	0.805430	1.340187e-49	-8.615850e-47
Msa0169410	Msa0567610	0.839475	1.549560e-57	-8.615850e-47
Msa0169410	Msa0574900	0.833905	4.076378e-56	-8.615850e-47
Msa0169410	Msa0579160	0.803072	4.151085e-49	-8.615850e-47
Msa0169410	Msa0579220	0.804076	2.569082e-49	-8.615850e-47
Msa0169410	Msa0601720	0.802943	4.413301e-49	-8.615850e-47
Msa0169410	Msa0607800	0.811616	6.403965e-51	-8.615850e-47
Msa0169410	Msa0608700	0.834448	2.980461e-56	-8.615850e-47
Msa0169410	Msa0626460	0.800493	1.404510e-48	-8.615850e-47
Msa0169410	Msa0630440	0.817073	3.983420e-52	-8.615850e-47
Msa0169410	Msa0641990	0.828793	7.377969e-55	-8.615850e-47
Msa0169410	Msa0648040	0.800325	1.519834e-48	-8.615850e-47
Msa0169410	Msa0649200	0.823339	1.460914e-53	-8.615850e-47
Msa0169410	Msa0652770	0.821628	3.648616e-53	-8.615850e-47
Msa0169410	Msa0660370	0.806831	6.796335e-50	-8.615850e-47
Msa0169410	Msa0681050	0.800431	1.446045e-48	-8.615850e-47
Msa0169410	Msa0690800	0.801624	8.245293e-49	-8.615850e-47
Msa0169410	Msa0692790	0.815284	1.000440e-51	-8.615850e-47
Msa0169410	Msa0703950	0.875829	2.262751e-68	-8.615850e-47
Msa0169410	Msa0709440	0.806267	8.939027e-50	-8.615850e-47
Msa0169410	Msa0716890	0.805100	1.571576e-49	-8.615850e-47
Msa0169410	Msa0734250	0.808390	3.171157e-50	-8.615850e-47
Msa0169410	Msa0745140	0.831398	1.707994e-55	-8.615850e-47
Msa0169410	Msa0746650	0.801634	8.208351e-49	-8.615850e-47
Msa0169410	Msa0754430	0.829529	4.892207e-55	-8.615850e-47
Msa0169410	Msa0754450	0.831416	1.689793e-55	-8.615850e-47
Msa0169410	Msa0756610	0.803911	2.781176e-49	-8.615850e-47
Msa0169410	Msa0776640	0.831784	1.371403e-55	-8.615850e-47
Msa0169410	Msa0795750	0.810010	1.425775e-50	-8.615850e-47
Msa0169410	Msa0813160	0.828247	9.993983e-55	-8.615850e-47
Msa0169410	Msa0825920	0.825631	4.218336e-54	-8.615850e-47
Msa0169410	Msa0835930	0.806155	9.435463e-50	-8.615850e-47
Msa0169410	Msa0851850	0.834065	3.717813e-56	-8.615850e-47
Msa0169410	Msa0865420	0.839910	1.194183e-57	-8.615850e-47
Msa0169410	Msa0865450	0.818781	1.639014e-52	-8.615850e-47
Msa0169410	Msa0878280	0.824872	6.376902e-54	-8.615850e-47
Msa0169410	Msa0902360	0.808816	2.571557e-50	-8.615850e-47
Msa0169410	Msa0930770	0.805650	1.204729e-49	-8.615850e-47
Msa0169410	Msa0944010	0.810262	1.258227e-50	-8.615850e-47
Msa0169410	Msa0945190	0.814429	1.547506e-51	-8.615850e-47
Msa0169410	Msa0980290	0.812328	4.481109e-51	-8.615850e-47
Msa0169410	Msa1002350	0.865363	6.233543e-65	-8.615850e-47
Msa0169410	Msa1044250	0.802130	6.492641e-49	-8.615850e-47
Msa0169410	Msa1072940	0.802385	5.754234e-49	-8.615850e-47
Msa0169410	Msa1074900	0.823870	1.097303e-53	-8.615850e-47
Msa0169410	Msa1078830	0.835780	1.375147e-56	-8.615850e-47
Msa0169410	Msa1078890	0.834820	2.402796e-56	-8.615850e-47
Msa0169410	Msa1082410	0.829885	4.007134e-55	-8.615850e-47
Msa0169410	Msa1083700	0.809861	1.535031e-50	-8.615850e-47
Msa0169410	Msa1106590	0.804218	2.400948e-49	-8.615850e-47
Msa0169410	Msa1125990	0.801652	8.139829e-49	-8.615850e-47
Msa0169410	Msa1145300	0.820298	7.384570e-53	-8.615850e-47
Msa0169410	Msa1147700	0.815791	7.714313e-52	-8.615850e-47
Msa0169410	Msa1160920	0.808971	2.382715e-50	-8.615850e-47
Msa0169410	Msa1165090	0.823763	1.162728e-53	-8.615850e-47
Msa0169410	Msa1169660	0.840198	1.004303e-57	-8.615850e-47
Msa0169410	Msa1171290	0.814155	1.779605e-51	-8.615850e-47
Msa0169410	Msa1201170	0.800043	1.734577e-48	-8.615850e-47
Msa0169410	Msa1201780	0.806563	7.739803e-50	-8.615850e-47
Msa0169410	Msa1207190	0.809799	1.583000e-50	-8.615850e-47
Msa0169410	Msa1207330	0.824470	7.934695e-54	-8.615850e-47
Msa0169410	Msa1257440	0.823114	1.648474e-53	-8.615850e-47
Msa0169410	Msa1257570	0.825045	5.807505e-54	-8.615850e-47
Msa0169410	Msa1305250	0.823374	1.433496e-53	-8.615850e-47
Msa0169410	Msa1331300	0.816434	5.540653e-52	-8.615850e-47
Msa0169410	Msa1347390	0.804868	1.757093e-49	-8.615850e-47
Msa0169410	Msa1365820	0.827562	1.461093e-54	-8.615850e-47
Msa0169410	Msa1366150	0.838405	2.932195e-57	-8.615850e-47
Msa0169410	Msa1368390	0.812555	3.997485e-51	-8.615850e-47
Msa0169410	Msa1375280	0.807527	4.838710e-50	-8.615850e-47
Msa0169410	Msa1381260	0.811810	5.811354e-51	-8.615850e-47
Msa0169410	Msa1387440	0.820790	5.692816e-53	-8.615850e-47
Msa0169410	Msa1391500	0.855321	6.821923e-62	-8.615850e-47
Msa0169410	Msa1398810	0.871369	7.203423e-67	-8.615850e-47
Msa0169410	Msa1407840	0.855200	7.398181e-62	-8.615850e-47
Msa0169410	Msa1412000	0.824176	9.301001e-54	-8.615850e-47
Msa0169410	Msa1427110	0.814293	1.658658e-51	-8.615850e-47
Msa0169410	Msa1429070	0.822401	2.416076e-53	-8.615850e-47
Msa0169410	Msa1437810	0.854154	1.486411e-61	-8.615850e-47
Msa0169410	Msa1441960	0.806556	7.767740e-50	-8.615850e-47
Msa0169410	Msa1447100	0.820723	5.898525e-53	-8.615850e-47
Msa0169410	Msa1454500	0.844038	9.681919e-59	-8.615850e-47
Msa0169410	Msa1457770	0.801569	8.462820e-49	-8.615850e-47
Msa0169410	Msa1457890	0.824196	9.202670e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0169410	MtrunA17_Chr1g0186151	98.418	316	5	0	1	316	1	316	0.0	632
Msa0169410	MtrunA17_Chr1g0168311	69.868	302	75	3	1	298	1	290	1.90e-146	414
Msa0169410	MtrunA17_Chr7g0262011	48.789	289	131	4	11	298	83	355	1.65e-88	269
Msa0169410	MtrunA17_Chr1g0190321	39.130	299	156	9	12	298	10	294	2.57e-63	202
Msa0169410	MtrunA17_Chr5g0434531	29.553	291	185	5	9	297	4	276	3.49e-34	126
Msa0169410	MtrunA17_Chr3g0110311	30.822	292	172	6	6	293	5	270	1.07e-33	124
Msa0169410	MtrunA17_Chr7g0230381	28.826	281	166	8	5	275	11	267	1.51e-30	116
Msa0169410	MtrunA17_Chr8g0391511	28.881	277	182	5	26	299	25	289	1.79e-30	116
Msa0169410	MtrunA17_Chr7g0234231	22.835	254	158	4	8	241	4	239	1.17e-16	78.2
Msa0169410	MtrunA17_Chr1g0205771	25.769	260	166	8	47	290	54	302	1.37e-15	77.4
Msa0169410	MtrunA17_Chr1g0206291	25.101	247	160	9	9	237	23	262	5.99e-15	75.5
Msa0169410	MtrunA17_Chr6g0475001	24.149	323	188	11	1	281	1	308	9.77e-15	74.7
Msa0169410	MtrunA17_Chr5g0394001	26.168	214	143	4	38	237	35	247	3.29e-14	73.2
Msa0169410	MtrunA17_Chr3g0109411	27.679	224	149	5	21	234	18	238	4.61e-14	72.8
Msa0169410	MtrunA17_Chr6g0474901	23.462	260	159	5	44	270	33	285	9.32e-14	72.0
Msa0169410	MtrunA17_Chr6g0475201	23.108	251	175	6	1	237	1	247	2.08e-13	70.9
Msa0169410	MtrunA17_Chr3g0081001	22.785	237	170	5	8	234	7	240	2.02e-12	67.8
Msa0169410	MtrunA17_Chr5g0428531	25.103	243	160	9	9	236	9	244	2.26e-12	66.6
Msa0169410	MtrunA17_Chr5g0394021	23.045	243	154	6	68	292	68	295	2.32e-12	67.8
Msa0169410	MtrunA17_Chr1g0206351	25.541	231	135	7	32	237	33	251	2.77e-12	67.4
Msa0169410	MtrunA17_Chr6g0475091	26.050	238	143	7	23	237	30	257	4.08e-12	67.0
Msa0169410	MtrunA17_Chr6g0474871	25.581	301	172	12	6	270	5	289	1.33e-11	65.5
Msa0169410	MtrunA17_Chr6g0474861	25.000	240	161	7	11	236	6	240	3.15e-11	64.3
Msa0169410	MtrunA17_Chr6g0474971	24.809	262	153	9	44	270	33	285	3.40e-11	63.2
Msa0169410	MtrunA17_Chr3g0081031	23.792	269	167	9	12	252	22	280	4.70e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0169410	AT1G04520.1	67.230	296	89	2	8	297	10	303	1.27e-135	387
Msa0169410	AT2G33330.1	65.116	301	100	3	1	297	1	300	9.08e-134	382
Msa0169410	AT5G43980.1	44.330	291	136	5	25	300	20	299	7.48e-78	240
Msa0169410	AT3G04370.1	35.738	305	154	6	24	298	25	317	7.97e-59	192
Msa0169410	AT3G04370.2	35.197	304	156	5	24	298	25	316	9.01e-59	191
Msa0169410	AT5G37660.1	30.996	271	168	6	11	279	15	268	4.51e-34	126
Msa0169410	AT5G37660.2	30.996	271	168	6	11	279	15	268	5.23e-34	126
Msa0169410	AT3G60720.3	29.044	272	165	6	33	296	29	280	2.68e-31	119
Msa0169410	AT3G60720.1	28.889	270	164	6	33	294	29	278	2.83e-30	116
Msa0169410	AT2G01660.3	29.966	297	185	9	11	300	7	287	5.05e-29	113
Msa0169410	AT2G01660.1	30.201	298	184	10	11	300	7	288	5.54e-29	113
Msa0169410	AT2G01660.2	31.034	232	142	7	26	250	20	240	7.82e-26	103
Msa0169410	AT1G70690.1	28.571	301	183	8	10	296	9	291	2.05e-24	100
Msa0169410	AT3G60720.2	27.885	208	139	4	33	233	29	232	7.11e-24	98.2
Msa0169410	AT5G48540.1	23.077	221	156	5	34	241	24	243	1.88e-16	78.2
Msa0169410	AT5G40380.1	27.946	297	175	12	9	296	12	278	4.55e-15	76.3
Msa0169410	AT5G40380.3	27.946	297	175	12	9	296	12	278	5.43e-15	75.9
Msa0169410	AT5G40380.2	27.946	297	175	12	9	296	12	278	5.80e-15	75.9
Msa0169410	AT4G05200.1	23.125	320	221	10	12	309	11	327	8.97e-15	75.5
Msa0169410	AT4G05200.2	23.125	320	221	10	12	309	29	345	9.16e-15	75.5
Msa0169410	AT5G41290.1	24.468	282	192	6	6	272	5	280	7.87e-13	68.2

Find 88 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGCAAGAGTTCAGCTTTC+AGG	0.163538	1_4:-52647744	Msa0169410:CDS
CCCCAGATGAATGAATATTT+TGG	0.173830	1_4:-52646335	Msa0169410:three_prime_UTR
GCTCTCTCTTCTTCTGGTTT+TGG	0.207236	1_4:+52648334	None:intergenic
TGCATTGCTCTCTCTTCTTC+TGG	0.207247	1_4:+52648328	None:intergenic
TCTGGTTACTCTTCATCTTT+TGG	0.217123	1_4:-52647330	Msa0169410:CDS
AGATACCTGTGATGCTATTT+TGG	0.228242	1_4:+52647859	None:intergenic
ATCCAAAATATTCATTCATC+TGG	0.267270	1_4:+52646333	None:intergenic
GGAAAAGAATCAAATGGTTT+TGG	0.277426	1_4:+52648244	None:intergenic
GAATGTGGAAGTTCTATTTC+AGG	0.278336	1_4:-52647450	Msa0169410:CDS
GATTCTTGATATATATCTTT+TGG	0.281699	1_4:-52646541	Msa0169410:three_prime_UTR
GAATCAAAACATGGTTGTTT+TGG	0.289029	1_4:+52648140	None:intergenic
AAGACTGTGGCAATAATATT+AGG	0.324267	1_4:-52646822	Msa0169410:CDS
CCACCCTTTGGAACCCCATT+AGG	0.332103	1_4:+52647384	None:intergenic
AATCCACCTCAAGAAGAATT+TGG	0.332910	1_4:+52646564	None:intergenic
GGTGGTGGATCATCCTCTTC+TGG	0.358061	1_4:-52647348	Msa0169410:CDS
TAGACCAAAGTTGTGTAATC+AGG	0.371990	1_4:+52648011	None:intergenic
AAGATGATGATTTAGAGAAA+TGG	0.375671	1_4:+52648036	None:intergenic
CAAGACTAATTCTTGTAGTT+AGG	0.381469	1_4:-52646450	Msa0169410:three_prime_UTR
GATTGTGAATATGCACCATT+TGG	0.394933	1_4:+52647957	None:intergenic
AGATGATGATTTAGAGAAAT+GGG	0.405156	1_4:+52648037	None:intergenic
AAGGGTGGTCACTCTTCATC+TGG	0.416746	1_4:-52647369	Msa0169410:CDS
TTCTCCTGATTACACAACTT+TGG	0.429549	1_4:-52648015	Msa0169410:CDS
TCCAAAATATTCATTCATCT+GGG	0.437790	1_4:+52646334	None:intergenic
TGGTATCATTACAAGGGAAA+AGG	0.439290	1_4:-52646365	Msa0169410:three_prime_UTR
GAAGAACAAAAGAAGAAAGA+GGG	0.456695	1_4:+52648073	None:intergenic
TTATCAAGCTTTGTATGTGA+TGG	0.456880	1_4:-52647547	Msa0169410:CDS
AGGTTCTTCAAAACAACAAC+TGG	0.461798	1_4:-52647888	Msa0169410:CDS
AATGGTGTTGTGAGTAATCA+TGG	0.467963	1_4:-52647585	Msa0169410:CDS
CATAGTTTGTCTGAGAGAAC+TGG	0.468018	1_4:+52647780	None:intergenic
ACAACAAATGCTGCTGGAAG+AGG	0.468669	1_4:-52647648	Msa0169410:CDS
TACATGCTGTACGAGGTTGC+TGG	0.468867	1_4:-52647717	Msa0169410:CDS
GGGAGATAGAGAAAGAATCT+AGG	0.469905	1_4:-52646490	Msa0169410:three_prime_UTR
GGTCAGTGTGAGGGTGATGT+TGG	0.474760	1_4:-52647525	Msa0169410:CDS
GATGAAGAGTGACCACCCTT+TGG	0.481028	1_4:+52647372	None:intergenic
AAGCTGAACTCTTGCAGAGA+TGG	0.484956	1_4:+52647749	None:intergenic
CAGATTCTCTTCAAGACTTG+TGG	0.485266	1_4:-52647672	Msa0169410:CDS
GGTCACTCTTCATCTGGTGG+TGG	0.489663	1_4:-52647363	Msa0169410:CDS
TGAAGAACAAAAGAAGAAAG+AGG	0.494984	1_4:+52648072	None:intergenic
TTCAAAACAACAACTGGTAA+TGG	0.498720	1_4:-52647882	Msa0169410:CDS
TTGTGTTGAGATTCAGAGAG+GGG	0.500297	1_4:+52648209	None:intergenic
GGTTAAGGAAAAGAATCAAA+TGG	0.504695	1_4:+52648238	None:intergenic
TGTGGAACAACAAATGCTGC+TGG	0.508236	1_4:-52647654	Msa0169410:CDS
AGATTTGTGGTAAATAGACT+TGG	0.510548	1_4:-52646511	Msa0169410:three_prime_UTR
TTGTGAGATCTCCTCTGCAT+TGG	0.514398	1_4:+52647835	None:intergenic
AATGGCCAAAATAGCATCAC+AGG	0.519556	1_4:-52647864	Msa0169410:CDS
AATCTAGGACTGTTGAATGT+GGG	0.519681	1_4:-52646475	Msa0169410:three_prime_UTR
CAAAATATTCATTCATCTGG+GGG	0.529568	1_4:+52646336	None:intergenic
GCTATGACAGGACAAAATAC+AGG	0.532912	1_4:-52646846	Msa0169410:intron
ATCTGCATCCCTGAAACTTG+TGG	0.533086	1_4:+52647690	None:intergenic
TTATCCTAATGGGGTTCCAA+AGG	0.535083	1_4:-52647388	Msa0169410:CDS
TATCAAGCTTTGTATGTGAT+GGG	0.536644	1_4:-52647546	Msa0169410:CDS
GATGTTGGTGATTCTGATTG+TGG	0.539277	1_4:-52647510	Msa0169410:CDS
TATCCTAATGGGGTTCCAAA+GGG	0.541401	1_4:-52647387	Msa0169410:CDS
GAAACATTCACAGATCCAAA+TGG	0.542666	1_4:-52647972	Msa0169410:CDS
TTATATGCTTGCTATTTGCA+AGG	0.544026	1_4:-52646773	Msa0169410:CDS
AGATGAAGAGTAACCAGAAG+AGG	0.557396	1_4:+52647335	None:intergenic
TTATTGACTGTAGAAGAAGA+TGG	0.558415	1_4:-52646600	Msa0169410:CDS
AGGCCAAATTCTTCTTGAGG+TGG	0.559342	1_4:-52646567	Msa0169410:exon
GAATCTAGGACTGTTGAATG+TGG	0.563094	1_4:-52646476	Msa0169410:three_prime_UTR
TACACAACTTTGGTCTACAA+AGG	0.569043	1_4:-52648005	Msa0169410:CDS
GGTGGTCACTCTTCATCTGG+TGG	0.573580	1_4:-52647366	Msa0169410:CDS
TGTGTTGAGATTCAGAGAGG+GGG	0.574643	1_4:+52648210	None:intergenic
GATTTGTGGTAAATAGACTT+GGG	0.575361	1_4:-52646510	Msa0169410:three_prime_UTR
CAACAAATGCTGCTGGAAGA+GGG	0.576463	1_4:-52647647	Msa0169410:CDS
TATGTGATGGGTCAGTGTGA+GGG	0.578918	1_4:-52647534	Msa0169410:CDS
TTTGTGTTGAGATTCAGAGA+GGG	0.582808	1_4:+52648208	None:intergenic
CCAAAATATTCATTCATCTG+GGG	0.584922	1_4:+52646335	None:intergenic
GTATGTGATGGGTCAGTGTG+AGG	0.592839	1_4:-52647535	Msa0169410:CDS
AAGAACAAAAGAAGAAAGAG+GGG	0.594004	1_4:+52648074	None:intergenic
CAAAGAGCTCAAGTTGAATG+TGG	0.597170	1_4:-52647465	Msa0169410:CDS
TGGAGGCCAAATTCTTCTTG+AGG	0.599401	1_4:-52646570	Msa0169410:exon
AAGAAGATGGTTTACTGTGG+AGG	0.604602	1_4:-52646587	Msa0169410:CDS
GTGTTGAGATTCAGAGAGGG+GGG	0.605267	1_4:+52648211	None:intergenic
ATCTAGGACTGTTGAATGTG+GGG	0.608364	1_4:-52646474	Msa0169410:three_prime_UTR
ATTAGGAGGTATAGCTGCTG+TGG	0.619016	1_4:-52646805	Msa0169410:CDS
CCTAATGGGGTTCCAAAGGG+TGG	0.628137	1_4:-52647384	Msa0169410:CDS
TGTTGAGATTCAGAGAGGGG+GGG	0.631211	1_4:+52648212	None:intergenic
GTTTGTGTTGAGATTCAGAG+AGG	0.635997	1_4:+52648207	None:intergenic
ACAGGTATCTTCCAATGCAG+AGG	0.638244	1_4:-52647846	Msa0169410:CDS
ACTGTGGCAATAATATTAGG+AGG	0.641949	1_4:-52646819	Msa0169410:CDS
TGATGAAGAAACGTGATGGT+AGG	0.645756	1_4:-52646746	Msa0169410:intron
AGGTTGTTACATGCTGTACG+AGG	0.659964	1_4:-52647724	Msa0169410:CDS
AATTTGATGAAGAAACGTGA+TGG	0.662013	1_4:-52646750	Msa0169410:CDS
GTTCTCTCAGACAAACTATG+TGG	0.667348	1_4:-52647777	Msa0169410:CDS
ATGGAGAGAGAATCAAAACA+TGG	0.673903	1_4:+52648131	None:intergenic
TAGAAGAAGATGGTTTACTG+TGG	0.689143	1_4:-52646590	Msa0169410:CDS
AAAATATTCATTCATCTGGG+GGG	0.697623	1_4:+52646337	None:intergenic
ACAAAATACAGGAAAGACTG+TGG	0.709113	1_4:-52646835	Msa0169410:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTCTCAAAAACCAAAAAT+GGG	-	chr1_4:52646553-52646572	Msa0169410:exon	20.0%
!!!	AGTTTTAGTTATTATCCTAA+TGG	-	chr1_4:52647264-52647283	Msa0169410:intron	20.0%
!!!	GTTTTAGTTATTATCCTAAT+GGG	-	chr1_4:52647265-52647284	Msa0169410:intron	20.0%
!!!	TTTTAGTTATTATCCTAATG+GGG	-	chr1_4:52647266-52647285	Msa0169410:intron	20.0%
!!!	TTTTACTTTTATGTTGAATC+TGG	-	chr1_4:52647438-52647457	Msa0169410:CDS	20.0%
!!!	CAATTTTCTCAAATTATGAT+CGG	+	chr1_4:52647520-52647539	None:intergenic	20.0%
!!	TTATAACACATGAACAATTA+AGG	+	chr1_4:52647707-52647726	None:intergenic	20.0%
!!	GTTTCATACATATTAGTAAA+TGG	+	chr1_4:52647761-52647780	None:intergenic	20.0%
!!!	GATTCTTGATATATATCTTT+TGG	-	chr1_4:52648122-52648141	Msa0169410:five_prime_UTR	20.0%
!!	ATTTCTCAAAAACCAAAAAT+GGG	-	chr1_4:52646553-52646572	Msa0169410:exon	20.0%
!!!	AGTTTTAGTTATTATCCTAA+TGG	-	chr1_4:52647264-52647283	Msa0169410:intron	20.0%
!!!	GTTTTAGTTATTATCCTAAT+GGG	-	chr1_4:52647265-52647284	Msa0169410:intron	20.0%
!!!	TTTTAGTTATTATCCTAATG+GGG	-	chr1_4:52647266-52647285	Msa0169410:intron	20.0%
!!!	TTTTACTTTTATGTTGAATC+TGG	-	chr1_4:52647438-52647457	Msa0169410:CDS	20.0%
!!!	CAATTTTCTCAAATTATGAT+CGG	+	chr1_4:52647520-52647539	None:intergenic	20.0%
!!	TTATAACACATGAACAATTA+AGG	+	chr1_4:52647707-52647726	None:intergenic	20.0%
!!	GTTTCATACATATTAGTAAA+TGG	+	chr1_4:52647761-52647780	None:intergenic	20.0%
!!!	GATTCTTGATATATATCTTT+TGG	-	chr1_4:52648122-52648141	Msa0169410:five_prime_UTR	20.0%
!!!	AATCAAATGGTTTTGGTTTT+TGG	+	chr1_4:52646415-52646434	None:intergenic	25.0%
!!!	CATTTTTGGTTTTTGAGAAA+TGG	+	chr1_4:52646554-52646573	None:intergenic	25.0%
!	CATTTCTCAAAAACCAAAAA+TGG	-	chr1_4:52646552-52646571	Msa0169410:three_prime_UTR	25.0%
!!!	TTTTTTGGAAAACCCATTTT+TGG	+	chr1_4:52646568-52646587	None:intergenic	25.0%
!	AGATGATGATTTAGAGAAAT+GGG	+	chr1_4:52646629-52646648	None:intergenic	25.0%
!	AAGATGATGATTTAGAGAAA+TGG	+	chr1_4:52646630-52646649	None:intergenic	25.0%
!!!	TATCTATTGATTCTGAATGA+TGG	+	chr1_4:52647376-52647395	None:intergenic	25.0%
!	TCAGAATCAATAGATAAGAT+TGG	-	chr1_4:52647380-52647399	Msa0169410:CDS	25.0%
!!!	ATAAGATTGGTTTCAATTCT+GGG	-	chr1_4:52647393-52647412	Msa0169410:CDS	25.0%
!!!	TTGTACATGGTTTTACTATA+TGG	-	chr1_4:52647947-52647966	Msa0169410:CDS	25.0%
!!!	GATTTTGCTGTGAAAAAAAA+TGG	-	chr1_4:52648242-52648261	Msa0169410:five_prime_UTR	25.0%
!!!	TGAAAGTGAGAATTGTTTTT+TGG	-	chr1_4:52648278-52648297	Msa0169410:five_prime_UTR	25.0%
!!!	TGTTTTTTGGTATCATTACA+AGG	-	chr1_4:52648291-52648310	Msa0169410:five_prime_UTR	25.0%
!!!	GTTTTTTGGTATCATTACAA+GGG	-	chr1_4:52648292-52648311	Msa0169410:five_prime_UTR	25.0%
!	TCCAAAATATTCATTCATCT+GGG	+	chr1_4:52648332-52648351	None:intergenic	25.0%
!	ATCCAAAATATTCATTCATC+TGG	+	chr1_4:52648333-52648352	None:intergenic	25.0%
!!!	AATCAAATGGTTTTGGTTTT+TGG	+	chr1_4:52646415-52646434	None:intergenic	25.0%
!!!	CATTTTTGGTTTTTGAGAAA+TGG	+	chr1_4:52646554-52646573	None:intergenic	25.0%
!	CATTTCTCAAAAACCAAAAA+TGG	-	chr1_4:52646552-52646571	Msa0169410:three_prime_UTR	25.0%
!!!	TTTTTTGGAAAACCCATTTT+TGG	+	chr1_4:52646568-52646587	None:intergenic	25.0%
!	AGATGATGATTTAGAGAAAT+GGG	+	chr1_4:52646629-52646648	None:intergenic	25.0%
!	AAGATGATGATTTAGAGAAA+TGG	+	chr1_4:52646630-52646649	None:intergenic	25.0%
!!!	TATCTATTGATTCTGAATGA+TGG	+	chr1_4:52647376-52647395	None:intergenic	25.0%
!	TCAGAATCAATAGATAAGAT+TGG	-	chr1_4:52647380-52647399	Msa0169410:CDS	25.0%
!!!	ATAAGATTGGTTTCAATTCT+GGG	-	chr1_4:52647393-52647412	Msa0169410:CDS	25.0%
!!!	TTGTACATGGTTTTACTATA+TGG	-	chr1_4:52647947-52647966	Msa0169410:CDS	25.0%
!!!	GATTTTGCTGTGAAAAAAAA+TGG	-	chr1_4:52648242-52648261	Msa0169410:five_prime_UTR	25.0%
!!!	TGAAAGTGAGAATTGTTTTT+TGG	-	chr1_4:52648278-52648297	Msa0169410:five_prime_UTR	25.0%
!!!	TGTTTTTTGGTATCATTACA+AGG	-	chr1_4:52648291-52648310	Msa0169410:five_prime_UTR	25.0%
!!!	GTTTTTTGGTATCATTACAA+GGG	-	chr1_4:52648292-52648311	Msa0169410:five_prime_UTR	25.0%
!	TCCAAAATATTCATTCATCT+GGG	+	chr1_4:52648332-52648351	None:intergenic	25.0%
!	ATCCAAAATATTCATTCATC+TGG	+	chr1_4:52648333-52648352	None:intergenic	25.0%
!!!	ATGGTTTTGGTTTTTGGTTT+GGG	+	chr1_4:52646409-52646428	None:intergenic	30.0%
!!!	AATGGTTTTGGTTTTTGGTT+TGG	+	chr1_4:52646410-52646429	None:intergenic	30.0%
!	GGAAAAGAATCAAATGGTTT+TGG	+	chr1_4:52646422-52646441	None:intergenic	30.0%
	GGTTAAGGAAAAGAATCAAA+TGG	+	chr1_4:52646428-52646447	None:intergenic	30.0%
!!!	TTTGGTTTTTGGAGGAAAAA+AGG	+	chr1_4:52646508-52646527	None:intergenic	30.0%
!!!	AACATGGTTGTTTTGGTTTT+TGG	+	chr1_4:52646519-52646538	None:intergenic	30.0%
!	GAATCAAAACATGGTTGTTT+TGG	+	chr1_4:52646526-52646545	None:intergenic	30.0%
	AAGAACAAAAGAAGAAAGAG+GGG	+	chr1_4:52646592-52646611	None:intergenic	30.0%
	GAAGAACAAAAGAAGAAAGA+GGG	+	chr1_4:52646593-52646612	None:intergenic	30.0%
	TGAAGAACAAAAGAAGAAAG+AGG	+	chr1_4:52646594-52646613	None:intergenic	30.0%
!!	TTTTGTGGATTGTGAAACTA+AGG	+	chr1_4:52646752-52646771	None:intergenic	30.0%
	TTTCACAATCCACAAAAACA+AGG	-	chr1_4:52646755-52646774	Msa0169410:CDS	30.0%
!!!	TTTGAAGAACCTTGTTTTTG+TGG	+	chr1_4:52646767-52646786	None:intergenic	30.0%
	TTCAAAACAACAACTGGTAA+TGG	-	chr1_4:52646781-52646800	Msa0169410:CDS	30.0%
!	TTATCAAGCTTTGTATGTGA+TGG	-	chr1_4:52647116-52647135	Msa0169410:intron	30.0%
!	TATCAAGCTTTGTATGTGAT+GGG	-	chr1_4:52647117-52647136	Msa0169410:intron	30.0%
!!	GATAAGATTGGTTTCAATTC+TGG	-	chr1_4:52647392-52647411	Msa0169410:CDS	30.0%
!	ATTAATCATTGAGTTGTGTG+TGG	-	chr1_4:52647785-52647804	Msa0169410:CDS	30.0%
	AAGACTGTGGCAATAATATT+AGG	-	chr1_4:52647841-52647860	Msa0169410:CDS	30.0%
!!	TTATATGCTTGCTATTTGCA+AGG	-	chr1_4:52647890-52647909	Msa0169410:CDS	30.0%
	AATTTGATGAAGAAACGTGA+TGG	-	chr1_4:52647913-52647932	Msa0169410:CDS	30.0%
!	TTATTGACTGTAGAAGAAGA+TGG	-	chr1_4:52648063-52648082	Msa0169410:CDS	30.0%
	AGATTTGTGGTAAATAGACT+TGG	-	chr1_4:52648152-52648171	Msa0169410:five_prime_UTR	30.0%
	GATTTGTGGTAAATAGACTT+GGG	-	chr1_4:52648153-52648172	Msa0169410:five_prime_UTR	30.0%
	CAAGACTAATTCTTGTAGTT+AGG	-	chr1_4:52648213-52648232	Msa0169410:five_prime_UTR	30.0%
	AAAATATTCATTCATCTGGG+GGG	+	chr1_4:52648329-52648348	None:intergenic	30.0%
	CAAAATATTCATTCATCTGG+GGG	+	chr1_4:52648330-52648349	None:intergenic	30.0%
	CCAAAATATTCATTCATCTG+GGG	+	chr1_4:52648331-52648350	None:intergenic	30.0%
!!!	ATGGTTTTGGTTTTTGGTTT+GGG	+	chr1_4:52646409-52646428	None:intergenic	30.0%
!!!	AATGGTTTTGGTTTTTGGTT+TGG	+	chr1_4:52646410-52646429	None:intergenic	30.0%
!	GGAAAAGAATCAAATGGTTT+TGG	+	chr1_4:52646422-52646441	None:intergenic	30.0%
	GGTTAAGGAAAAGAATCAAA+TGG	+	chr1_4:52646428-52646447	None:intergenic	30.0%
!!!	TTTGGTTTTTGGAGGAAAAA+AGG	+	chr1_4:52646508-52646527	None:intergenic	30.0%
!!!	AACATGGTTGTTTTGGTTTT+TGG	+	chr1_4:52646519-52646538	None:intergenic	30.0%
!	GAATCAAAACATGGTTGTTT+TGG	+	chr1_4:52646526-52646545	None:intergenic	30.0%
	AAGAACAAAAGAAGAAAGAG+GGG	+	chr1_4:52646592-52646611	None:intergenic	30.0%
	GAAGAACAAAAGAAGAAAGA+GGG	+	chr1_4:52646593-52646612	None:intergenic	30.0%
	TGAAGAACAAAAGAAGAAAG+AGG	+	chr1_4:52646594-52646613	None:intergenic	30.0%
!!	TTTTGTGGATTGTGAAACTA+AGG	+	chr1_4:52646752-52646771	None:intergenic	30.0%
	TTTCACAATCCACAAAAACA+AGG	-	chr1_4:52646755-52646774	Msa0169410:CDS	30.0%
!!!	TTTGAAGAACCTTGTTTTTG+TGG	+	chr1_4:52646767-52646786	None:intergenic	30.0%
	TTCAAAACAACAACTGGTAA+TGG	-	chr1_4:52646781-52646800	Msa0169410:CDS	30.0%
!	TTATCAAGCTTTGTATGTGA+TGG	-	chr1_4:52647116-52647135	Msa0169410:intron	30.0%
!	TATCAAGCTTTGTATGTGAT+GGG	-	chr1_4:52647117-52647136	Msa0169410:intron	30.0%
!!	GATAAGATTGGTTTCAATTC+TGG	-	chr1_4:52647392-52647411	Msa0169410:CDS	30.0%
!	ATTAATCATTGAGTTGTGTG+TGG	-	chr1_4:52647785-52647804	Msa0169410:CDS	30.0%
	AAGACTGTGGCAATAATATT+AGG	-	chr1_4:52647841-52647860	Msa0169410:CDS	30.0%
!!	TTATATGCTTGCTATTTGCA+AGG	-	chr1_4:52647890-52647909	Msa0169410:CDS	30.0%
	AATTTGATGAAGAAACGTGA+TGG	-	chr1_4:52647913-52647932	Msa0169410:CDS	30.0%
!	TTATTGACTGTAGAAGAAGA+TGG	-	chr1_4:52648063-52648082	Msa0169410:CDS	30.0%
	AGATTTGTGGTAAATAGACT+TGG	-	chr1_4:52648152-52648171	Msa0169410:five_prime_UTR	30.0%
	GATTTGTGGTAAATAGACTT+GGG	-	chr1_4:52648153-52648172	Msa0169410:five_prime_UTR	30.0%
	CAAGACTAATTCTTGTAGTT+AGG	-	chr1_4:52648213-52648232	Msa0169410:five_prime_UTR	30.0%
	AAAATATTCATTCATCTGGG+GGG	+	chr1_4:52648329-52648348	None:intergenic	30.0%
	CAAAATATTCATTCATCTGG+GGG	+	chr1_4:52648330-52648349	None:intergenic	30.0%
	CCAAAATATTCATTCATCTG+GGG	+	chr1_4:52648331-52648350	None:intergenic	30.0%
!!!	TCTTCTGGTTTTGGTGTATT+GGG	+	chr1_4:52646323-52646342	None:intergenic	35.0%
!!!	TTCTTCTGGTTTTGGTGTAT+TGG	+	chr1_4:52646324-52646343	None:intergenic	35.0%
!!!	TTTTGGTTTGGGTTGATTGT+TGG	+	chr1_4:52646398-52646417	None:intergenic	35.0%
	TTTGTGTTGAGATTCAGAGA+GGG	+	chr1_4:52646458-52646477	None:intergenic	35.0%
!	TGGAGGAAAAAAGGTTTTCT+AGG	+	chr1_4:52646499-52646518	None:intergenic	35.0%
!!!	ATGGTTGTTTTGGTTTTTGG+AGG	+	chr1_4:52646516-52646535	None:intergenic	35.0%
	ATGGAGAGAGAATCAAAACA+TGG	+	chr1_4:52646535-52646554	None:intergenic	35.0%
!!!	AGAAGAAAGAGGGGTTTTTT+TGG	+	chr1_4:52646583-52646602	None:intergenic	35.0%
	TTCTCCTGATTACACAACTT+TGG	-	chr1_4:52646648-52646667	Msa0169410:intron	35.0%
	TAGACCAAAGTTGTGTAATC+AGG	+	chr1_4:52646655-52646674	None:intergenic	35.0%
	TACACAACTTTGGTCTACAA+AGG	-	chr1_4:52646658-52646677	Msa0169410:intron	35.0%
	GAAACATTCACAGATCCAAA+TGG	-	chr1_4:52646691-52646710	Msa0169410:intron	35.0%
	GATTGTGAATATGCACCATT+TGG	+	chr1_4:52646709-52646728	None:intergenic	35.0%
!	CAATCTCTTTCAGCACTTTT+TGG	-	chr1_4:52646724-52646743	Msa0169410:intron	35.0%
	AGGTTCTTCAAAACAACAAC+TGG	-	chr1_4:52646775-52646794	Msa0169410:CDS	35.0%
!!	AGATACCTGTGATGCTATTT+TGG	+	chr1_4:52646807-52646826	None:intergenic	35.0%
!!	GCTTTTTCTGTGATGGAAAA+TGG	-	chr1_4:52647060-52647079	Msa0169410:intron	35.0%
!!	AATGGTGTTGTGAGTAATCA+TGG	-	chr1_4:52647078-52647097	Msa0169410:intron	35.0%
	GAATGTGGAAGTTCTATTTC+AGG	-	chr1_4:52647213-52647232	Msa0169410:intron	35.0%
!	TCTGGTTACTCTTCATCTTT+TGG	-	chr1_4:52647333-52647352	Msa0169410:CDS	35.0%
!!	ATCGGTGTCAATGTTATGTT+AGG	+	chr1_4:52647502-52647521	None:intergenic	35.0%
!	ACCTTCAATCTGAAGTGTTA+AGG	-	chr1_4:52647629-52647648	Msa0169410:CDS	35.0%
	ACCTTAACACTTCAGATTGA+AGG	+	chr1_4:52647633-52647652	None:intergenic	35.0%
	GAAGTAGCTCAATTTAGCAA+TGG	-	chr1_4:52647671-52647690	Msa0169410:CDS	35.0%
	ACAAAATACAGGAAAGACTG+TGG	-	chr1_4:52647828-52647847	Msa0169410:CDS	35.0%
	ACTGTGGCAATAATATTAGG+AGG	-	chr1_4:52647844-52647863	Msa0169410:CDS	35.0%
	TAGAAGAAGATGGTTTACTG+TGG	-	chr1_4:52648073-52648092	Msa0169410:CDS	35.0%
	AATCCACCTCAAGAAGAATT+TGG	+	chr1_4:52648102-52648121	None:intergenic	35.0%
!!	TTTTGGCTGAAAGAGATTTG+TGG	-	chr1_4:52648139-52648158	Msa0169410:five_prime_UTR	35.0%
!	AATCTAGGACTGTTGAATGT+GGG	-	chr1_4:52648188-52648207	Msa0169410:five_prime_UTR	35.0%
	TGGTATCATTACAAGGGAAA+AGG	-	chr1_4:52648298-52648317	Msa0169410:five_prime_UTR	35.0%
!	CCCCAGATGAATGAATATTT+TGG	-	chr1_4:52648328-52648347	Msa0169410:five_prime_UTR	35.0%
!!!	TCTTCTGGTTTTGGTGTATT+GGG	+	chr1_4:52646323-52646342	None:intergenic	35.0%
!!!	TTCTTCTGGTTTTGGTGTAT+TGG	+	chr1_4:52646324-52646343	None:intergenic	35.0%
!!!	TTTTGGTTTGGGTTGATTGT+TGG	+	chr1_4:52646398-52646417	None:intergenic	35.0%
	TTTGTGTTGAGATTCAGAGA+GGG	+	chr1_4:52646458-52646477	None:intergenic	35.0%
!	TGGAGGAAAAAAGGTTTTCT+AGG	+	chr1_4:52646499-52646518	None:intergenic	35.0%
!!!	ATGGTTGTTTTGGTTTTTGG+AGG	+	chr1_4:52646516-52646535	None:intergenic	35.0%
	ATGGAGAGAGAATCAAAACA+TGG	+	chr1_4:52646535-52646554	None:intergenic	35.0%
!!!	AGAAGAAAGAGGGGTTTTTT+TGG	+	chr1_4:52646583-52646602	None:intergenic	35.0%
	TTCTCCTGATTACACAACTT+TGG	-	chr1_4:52646648-52646667	Msa0169410:intron	35.0%
	TAGACCAAAGTTGTGTAATC+AGG	+	chr1_4:52646655-52646674	None:intergenic	35.0%
	TACACAACTTTGGTCTACAA+AGG	-	chr1_4:52646658-52646677	Msa0169410:intron	35.0%
	GAAACATTCACAGATCCAAA+TGG	-	chr1_4:52646691-52646710	Msa0169410:intron	35.0%
	GATTGTGAATATGCACCATT+TGG	+	chr1_4:52646709-52646728	None:intergenic	35.0%
!	CAATCTCTTTCAGCACTTTT+TGG	-	chr1_4:52646724-52646743	Msa0169410:intron	35.0%
	AGGTTCTTCAAAACAACAAC+TGG	-	chr1_4:52646775-52646794	Msa0169410:CDS	35.0%
!!	AGATACCTGTGATGCTATTT+TGG	+	chr1_4:52646807-52646826	None:intergenic	35.0%
!!	GCTTTTTCTGTGATGGAAAA+TGG	-	chr1_4:52647060-52647079	Msa0169410:intron	35.0%
!!	AATGGTGTTGTGAGTAATCA+TGG	-	chr1_4:52647078-52647097	Msa0169410:intron	35.0%
	GAATGTGGAAGTTCTATTTC+AGG	-	chr1_4:52647213-52647232	Msa0169410:intron	35.0%
!	TCTGGTTACTCTTCATCTTT+TGG	-	chr1_4:52647333-52647352	Msa0169410:CDS	35.0%
!!	ATCGGTGTCAATGTTATGTT+AGG	+	chr1_4:52647502-52647521	None:intergenic	35.0%
!	ACCTTCAATCTGAAGTGTTA+AGG	-	chr1_4:52647629-52647648	Msa0169410:CDS	35.0%
	ACCTTAACACTTCAGATTGA+AGG	+	chr1_4:52647633-52647652	None:intergenic	35.0%
	GAAGTAGCTCAATTTAGCAA+TGG	-	chr1_4:52647671-52647690	Msa0169410:CDS	35.0%
	ACAAAATACAGGAAAGACTG+TGG	-	chr1_4:52647828-52647847	Msa0169410:CDS	35.0%
	ACTGTGGCAATAATATTAGG+AGG	-	chr1_4:52647844-52647863	Msa0169410:CDS	35.0%
	TAGAAGAAGATGGTTTACTG+TGG	-	chr1_4:52648073-52648092	Msa0169410:CDS	35.0%
	AATCCACCTCAAGAAGAATT+TGG	+	chr1_4:52648102-52648121	None:intergenic	35.0%
!!	TTTTGGCTGAAAGAGATTTG+TGG	-	chr1_4:52648139-52648158	Msa0169410:five_prime_UTR	35.0%
!	AATCTAGGACTGTTGAATGT+GGG	-	chr1_4:52648188-52648207	Msa0169410:five_prime_UTR	35.0%
	TGGTATCATTACAAGGGAAA+AGG	-	chr1_4:52648298-52648317	Msa0169410:five_prime_UTR	35.0%
!	CCCCAGATGAATGAATATTT+TGG	-	chr1_4:52648328-52648347	Msa0169410:five_prime_UTR	35.0%
	TTGTGTTGAGATTCAGAGAG+GGG	+	chr1_4:52646457-52646476	None:intergenic	40.0%
	GTTTGTGTTGAGATTCAGAG+AGG	+	chr1_4:52646459-52646478	None:intergenic	40.0%
	AATGGCCAAAATAGCATCAC+AGG	-	chr1_4:52646799-52646818	Msa0169410:CDS	40.0%
!	CATAGTTTGTCTGAGAGAAC+TGG	+	chr1_4:52646886-52646905	None:intergenic	40.0%
	GTTCTCTCAGACAAACTATG+TGG	-	chr1_4:52646886-52646905	Msa0169410:intron	40.0%
!!	CTGGTTTTCCACAAGTTTCA+GGG	-	chr1_4:52646965-52646984	Msa0169410:intron	40.0%
	CAGATTCTCTTCAAGACTTG+TGG	-	chr1_4:52646991-52647010	Msa0169410:intron	40.0%
!!!	AGACACTGCTTTTTCTGTGA+TGG	-	chr1_4:52647053-52647072	Msa0169410:intron	40.0%
!!	GATGTTGGTGATTCTGATTG+TGG	-	chr1_4:52647153-52647172	Msa0169410:intron	40.0%
!!	CAAAGAGCTCAAGTTGAATG+TGG	-	chr1_4:52647198-52647217	Msa0169410:intron	40.0%
	TTATCCTAATGGGGTTCCAA+AGG	-	chr1_4:52647275-52647294	Msa0169410:intron	40.0%
	TATCCTAATGGGGTTCCAAA+GGG	-	chr1_4:52647276-52647295	Msa0169410:intron	40.0%
	AGATGAAGAGTAACCAGAAG+AGG	+	chr1_4:52647331-52647350	None:intergenic	40.0%
!	GGTTACTCTTCATCTTTTGG+TGG	-	chr1_4:52647336-52647355	Msa0169410:CDS	40.0%
!	GTCAAGTTGTTGTGTTGTGT+AGG	-	chr1_4:52647566-52647585	Msa0169410:CDS	40.0%
!	CTTCAGATTGAAGGTGTGTT+CGG	+	chr1_4:52647624-52647643	None:intergenic	40.0%
	TGGCTAAATGATGCTATGAC+AGG	-	chr1_4:52647805-52647824	Msa0169410:CDS	40.0%
	GCTATGACAGGACAAAATAC+AGG	-	chr1_4:52647817-52647836	Msa0169410:CDS	40.0%
	TGATGAAGAAACGTGATGGT+AGG	-	chr1_4:52647917-52647936	Msa0169410:CDS	40.0%
	AAGAAGATGGTTTACTGTGG+AGG	-	chr1_4:52648076-52648095	Msa0169410:CDS	40.0%
	GGGAGATAGAGAAAGAATCT+AGG	-	chr1_4:52648173-52648192	Msa0169410:five_prime_UTR	40.0%
!	GAATCTAGGACTGTTGAATG+TGG	-	chr1_4:52648187-52648206	Msa0169410:five_prime_UTR	40.0%
!	ATCTAGGACTGTTGAATGTG+GGG	-	chr1_4:52648189-52648208	Msa0169410:five_prime_UTR	40.0%
	TTGTGTTGAGATTCAGAGAG+GGG	+	chr1_4:52646457-52646476	None:intergenic	40.0%
	GTTTGTGTTGAGATTCAGAG+AGG	+	chr1_4:52646459-52646478	None:intergenic	40.0%
	AATGGCCAAAATAGCATCAC+AGG	-	chr1_4:52646799-52646818	Msa0169410:CDS	40.0%
!	CATAGTTTGTCTGAGAGAAC+TGG	+	chr1_4:52646886-52646905	None:intergenic	40.0%
	GTTCTCTCAGACAAACTATG+TGG	-	chr1_4:52646886-52646905	Msa0169410:intron	40.0%
!!	CTGGTTTTCCACAAGTTTCA+GGG	-	chr1_4:52646965-52646984	Msa0169410:intron	40.0%
	CAGATTCTCTTCAAGACTTG+TGG	-	chr1_4:52646991-52647010	Msa0169410:intron	40.0%
!!!	AGACACTGCTTTTTCTGTGA+TGG	-	chr1_4:52647053-52647072	Msa0169410:intron	40.0%
!!	GATGTTGGTGATTCTGATTG+TGG	-	chr1_4:52647153-52647172	Msa0169410:intron	40.0%
!!	CAAAGAGCTCAAGTTGAATG+TGG	-	chr1_4:52647198-52647217	Msa0169410:intron	40.0%
	TTATCCTAATGGGGTTCCAA+AGG	-	chr1_4:52647275-52647294	Msa0169410:intron	40.0%
	TATCCTAATGGGGTTCCAAA+GGG	-	chr1_4:52647276-52647295	Msa0169410:intron	40.0%
	AGATGAAGAGTAACCAGAAG+AGG	+	chr1_4:52647331-52647350	None:intergenic	40.0%
!	GGTTACTCTTCATCTTTTGG+TGG	-	chr1_4:52647336-52647355	Msa0169410:CDS	40.0%
!	GTCAAGTTGTTGTGTTGTGT+AGG	-	chr1_4:52647566-52647585	Msa0169410:CDS	40.0%
!	CTTCAGATTGAAGGTGTGTT+CGG	+	chr1_4:52647624-52647643	None:intergenic	40.0%
	TGGCTAAATGATGCTATGAC+AGG	-	chr1_4:52647805-52647824	Msa0169410:CDS	40.0%
	GCTATGACAGGACAAAATAC+AGG	-	chr1_4:52647817-52647836	Msa0169410:CDS	40.0%
	TGATGAAGAAACGTGATGGT+AGG	-	chr1_4:52647917-52647936	Msa0169410:CDS	40.0%
	AAGAAGATGGTTTACTGTGG+AGG	-	chr1_4:52648076-52648095	Msa0169410:CDS	40.0%
	GGGAGATAGAGAAAGAATCT+AGG	-	chr1_4:52648173-52648192	Msa0169410:five_prime_UTR	40.0%
!	GAATCTAGGACTGTTGAATG+TGG	-	chr1_4:52648187-52648206	Msa0169410:five_prime_UTR	40.0%
!	ATCTAGGACTGTTGAATGTG+GGG	-	chr1_4:52648189-52648208	Msa0169410:five_prime_UTR	40.0%
!!!	GCTCTCTCTTCTTCTGGTTT+TGG	+	chr1_4:52646332-52646351	None:intergenic	45.0%
	TGCATTGCTCTCTCTTCTTC+TGG	+	chr1_4:52646338-52646357	None:intergenic	45.0%
	TGTGTTGAGATTCAGAGAGG+GGG	+	chr1_4:52646456-52646475	None:intergenic	45.0%
	ACAGGTATCTTCCAATGCAG+AGG	-	chr1_4:52646817-52646836	Msa0169410:CDS	45.0%
	TTGTGAGATCTCCTCTGCAT+TGG	+	chr1_4:52646831-52646850	None:intergenic	45.0%
	AAGCTGAACTCTTGCAGAGA+TGG	+	chr1_4:52646917-52646936	None:intergenic	45.0%
	TCTGCAAGAGTTCAGCTTTC+AGG	-	chr1_4:52646919-52646938	Msa0169410:intron	45.0%
	AGGTTGTTACATGCTGTACG+AGG	-	chr1_4:52646939-52646958	Msa0169410:intron	45.0%
!!!	GCTGGTTTTCCACAAGTTTC+AGG	-	chr1_4:52646964-52646983	Msa0169410:intron	45.0%
	ATCTGCATCCCTGAAACTTG+TGG	+	chr1_4:52646976-52646995	None:intergenic	45.0%
	TGTGGAACAACAAATGCTGC+TGG	-	chr1_4:52647009-52647028	Msa0169410:intron	45.0%
	ACAACAAATGCTGCTGGAAG+AGG	-	chr1_4:52647015-52647034	Msa0169410:intron	45.0%
	CAACAAATGCTGCTGGAAGA+GGG	-	chr1_4:52647016-52647035	Msa0169410:intron	45.0%
!!	TATGTGATGGGTCAGTGTGA+GGG	-	chr1_4:52647129-52647148	Msa0169410:intron	45.0%
	CAACTTGACAGTGATGCATG+TGG	+	chr1_4:52647555-52647574	None:intergenic	45.0%
!	ATTAGGAGGTATAGCTGCTG+TGG	-	chr1_4:52647858-52647877	Msa0169410:CDS	45.0%
!	TATAGCTGCTGTGGCGTTTT+TGG	-	chr1_4:52647867-52647886	Msa0169410:CDS	45.0%
!	GGTAGGTGTTGTGTTGTACA+TGG	-	chr1_4:52647934-52647953	Msa0169410:CDS	45.0%
	TGGAGGCCAAATTCTTCTTG+AGG	-	chr1_4:52648093-52648112	Msa0169410:CDS	45.0%
	AGGCCAAATTCTTCTTGAGG+TGG	-	chr1_4:52648096-52648115	Msa0169410:exon	45.0%
!!!	TCTGGTTTTGGTGTATTGGG+AGG	+	chr1_4:52646320-52646339	None:intergenic	45.0%
!!!	GCTCTCTCTTCTTCTGGTTT+TGG	+	chr1_4:52646332-52646351	None:intergenic	45.0%
	TGCATTGCTCTCTCTTCTTC+TGG	+	chr1_4:52646338-52646357	None:intergenic	45.0%
	TGTGTTGAGATTCAGAGAGG+GGG	+	chr1_4:52646456-52646475	None:intergenic	45.0%
	ACAGGTATCTTCCAATGCAG+AGG	-	chr1_4:52646817-52646836	Msa0169410:CDS	45.0%
	TTGTGAGATCTCCTCTGCAT+TGG	+	chr1_4:52646831-52646850	None:intergenic	45.0%
	AAGCTGAACTCTTGCAGAGA+TGG	+	chr1_4:52646917-52646936	None:intergenic	45.0%
	TCTGCAAGAGTTCAGCTTTC+AGG	-	chr1_4:52646919-52646938	Msa0169410:intron	45.0%
	AGGTTGTTACATGCTGTACG+AGG	-	chr1_4:52646939-52646958	Msa0169410:intron	45.0%
!!!	GCTGGTTTTCCACAAGTTTC+AGG	-	chr1_4:52646964-52646983	Msa0169410:intron	45.0%
	ATCTGCATCCCTGAAACTTG+TGG	+	chr1_4:52646976-52646995	None:intergenic	45.0%
	TGTGGAACAACAAATGCTGC+TGG	-	chr1_4:52647009-52647028	Msa0169410:intron	45.0%
	ACAACAAATGCTGCTGGAAG+AGG	-	chr1_4:52647015-52647034	Msa0169410:intron	45.0%
	CAACAAATGCTGCTGGAAGA+GGG	-	chr1_4:52647016-52647035	Msa0169410:intron	45.0%
!!	TATGTGATGGGTCAGTGTGA+GGG	-	chr1_4:52647129-52647148	Msa0169410:intron	45.0%
	CAACTTGACAGTGATGCATG+TGG	+	chr1_4:52647555-52647574	None:intergenic	45.0%
!	ATTAGGAGGTATAGCTGCTG+TGG	-	chr1_4:52647858-52647877	Msa0169410:CDS	45.0%
!	TATAGCTGCTGTGGCGTTTT+TGG	-	chr1_4:52647867-52647886	Msa0169410:CDS	45.0%
!	GGTAGGTGTTGTGTTGTACA+TGG	-	chr1_4:52647934-52647953	Msa0169410:CDS	45.0%
	TGGAGGCCAAATTCTTCTTG+AGG	-	chr1_4:52648093-52648112	Msa0169410:CDS	45.0%
	AGGCCAAATTCTTCTTGAGG+TGG	-	chr1_4:52648096-52648115	Msa0169410:exon	45.0%
	TGTTGAGATTCAGAGAGGGG+GGG	+	chr1_4:52646454-52646473	None:intergenic	50.0%
	GTGTTGAGATTCAGAGAGGG+GGG	+	chr1_4:52646455-52646474	None:intergenic	50.0%
	TACATGCTGTACGAGGTTGC+TGG	-	chr1_4:52646946-52646965	Msa0169410:intron	50.0%
!!	GTATGTGATGGGTCAGTGTG+AGG	-	chr1_4:52647128-52647147	Msa0169410:intron	50.0%
	GATGAAGAGTGACCACCCTT+TGG	+	chr1_4:52647294-52647313	None:intergenic	50.0%
	AAGGGTGGTCACTCTTCATC+TGG	-	chr1_4:52647294-52647313	Msa0169410:intron	50.0%
	TGTCTGACACCAACGCATGT+CGG	-	chr1_4:52647589-52647608	Msa0169410:CDS	50.0%
	TGCATGTATCCGACATGCGT+TGG	+	chr1_4:52647601-52647620	None:intergenic	50.0%
	TGTTGAGATTCAGAGAGGGG+GGG	+	chr1_4:52646454-52646473	None:intergenic	50.0%
	GTGTTGAGATTCAGAGAGGG+GGG	+	chr1_4:52646455-52646474	None:intergenic	50.0%
	TACATGCTGTACGAGGTTGC+TGG	-	chr1_4:52646946-52646965	Msa0169410:intron	50.0%
!!	GTATGTGATGGGTCAGTGTG+AGG	-	chr1_4:52647128-52647147	Msa0169410:intron	50.0%
	GATGAAGAGTGACCACCCTT+TGG	+	chr1_4:52647294-52647313	None:intergenic	50.0%
	AAGGGTGGTCACTCTTCATC+TGG	-	chr1_4:52647294-52647313	Msa0169410:intron	50.0%
	TGTCTGACACCAACGCATGT+CGG	-	chr1_4:52647589-52647608	Msa0169410:CDS	50.0%
	TGCATGTATCCGACATGCGT+TGG	+	chr1_4:52647601-52647620	None:intergenic	50.0%
	GGTCAGTGTGAGGGTGATGT+TGG	-	chr1_4:52647138-52647157	Msa0169410:intron	55.0%
!!	CCACCCTTTGGAACCCCATT+AGG	+	chr1_4:52647282-52647301	None:intergenic	55.0%
	CCTAATGGGGTTCCAAAGGG+TGG	-	chr1_4:52647279-52647298	Msa0169410:intron	55.0%
	GGTGGTCACTCTTCATCTGG+TGG	-	chr1_4:52647297-52647316	Msa0169410:intron	55.0%
	GGTCACTCTTCATCTGGTGG+TGG	-	chr1_4:52647300-52647319	Msa0169410:intron	55.0%
!!	GGTGGTGGATCATCCTCTTC+TGG	-	chr1_4:52647315-52647334	Msa0169410:intron	55.0%
	GGTCAGTGTGAGGGTGATGT+TGG	-	chr1_4:52647138-52647157	Msa0169410:intron	55.0%
!!	CCACCCTTTGGAACCCCATT+AGG	+	chr1_4:52647282-52647301	None:intergenic	55.0%
	CCTAATGGGGTTCCAAAGGG+TGG	-	chr1_4:52647279-52647298	Msa0169410:intron	55.0%
	GGTGGTCACTCTTCATCTGG+TGG	-	chr1_4:52647297-52647316	Msa0169410:intron	55.0%
	GGTCACTCTTCATCTGGTGG+TGG	-	chr1_4:52647300-52647319	Msa0169410:intron	55.0%
!!	GGTGGTGGATCATCCTCTTC+TGG	-	chr1_4:52647315-52647334	Msa0169410:intron	55.0%
	AGAGAGGGGGGGACAGGTTA+AGG	+	chr1_4:52646443-52646462	None:intergenic	60.0%
	AGATTCAGAGAGGGGGGGAC+AGG	+	chr1_4:52646449-52646468	None:intergenic	60.0%
	AGAGAGGGGGGGACAGGTTA+AGG	+	chr1_4:52646443-52646462	None:intergenic	60.0%
	AGATTCAGAGAGGGGGGGAC+AGG	+	chr1_4:52646449-52646468	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	52646319	52648366	52646319	ID=Msa0169410;Name=Msa0169410
chr1_4	mRNA	52646319	52648366	52646319	ID=Msa0169410-mRNA-1;Parent=Msa0169410;Name=Msa0169410-mRNA-1;_AED=0.04;_eAED=0.04;_QI=252\|0.5\|0.66\|1\|1\|1\|3\|253\|316
chr1_4	exon	52646319	52646623	52646319	ID=Msa0169410-mRNA-1:exon:15655;Parent=Msa0169410-mRNA-1
chr1_4	exon	52646747	52646858	52646747	ID=Msa0169410-mRNA-1:exon:15654;Parent=Msa0169410-mRNA-1
chr1_4	exon	52647328	52648366	52647328	ID=Msa0169410-mRNA-1:exon:15653;Parent=Msa0169410-mRNA-1
chr1_4	five_prime_UTR	52648115	52648366	52648115	ID=Msa0169410-mRNA-1:five_prime_utr;Parent=Msa0169410-mRNA-1
chr1_4	CDS	52647328	52648114	52647328	ID=Msa0169410-mRNA-1:cds;Parent=Msa0169410-mRNA-1
chr1_4	CDS	52646747	52646858	52646747	ID=Msa0169410-mRNA-1:cds;Parent=Msa0169410-mRNA-1
chr1_4	CDS	52646572	52646623	52646572	ID=Msa0169410-mRNA-1:cds;Parent=Msa0169410-mRNA-1
chr1_4	three_prime_UTR	52646319	52646571	52646319	ID=Msa0169410-mRNA-1:three_prime_utr;Parent=Msa0169410-mRNA-1

Gene Sequence

>Msa0169410

ATGGGTTTTCCAAAAAAACCCCTCTTTCTTCTTTTGTTCTTCATTTTCTTCACAACCCATTTCTCTAAATCATCATCTTCTCCTGATTACACAACTTTGGTCTACAAAGGTTGTTCAAAAGAAACATTCACAGATCCAAATGGTGCATATTCACAATCTCTTTCAGCACTTTTTGGTTCCTTAGTTTCACAATCCACAAAAACAAGGTTCTTCAAAACAACAACTGGTAATGGCCAAAATAGCATCACAGGTATCTTCCAATGCAGAGGAGATCTCACAAACTCAGATTGCTACAGCTGTGTTAGCAAGCTTCCAGTTCTCTCAGACAAACTATGTGGCAAAACCATCTCTGCAAGAGTTCAGCTTTCAGGTTGTTACATGCTGTACGAGGTTGCTGGTTTTCCACAAGTTTCAGGGATGCAGATTCTCTTCAAGACTTGTGGAACAACAAATGCTGCTGGAAGAGGGTTTGAAGAGAGAAGAGACACTGCTTTTTCTGTGATGGAAAATGGTGTTGTGAGTAATCATGGTTTTTATGTTACTACTTATCAAGCTTTGTATGTGATGGGTCAGTGTGAGGGTGATGTTGGTGATTCTGATTGTGGTGAGTGTGTTAAATCTGCTGTTCAAAGAGCTCAAGTTGAATGTGGAAGTTCTATTTCAGGACAAATTTATTTGCATAAGTGCTTTATTAGTTTTAGTTATTATCCTAATGGGGTTCCAAAGGGTGGTCACTCTTCATCTGGTGGTGGATCATCCTCTTCTGGTTACTCTTCATCTTTTGGTGGACAAAATACAGGAAAGACTGTGGCAATAATATTAGGAGGTATAGCTGCTGTGGCGTTTTTGGTTATATGCTTGCTATTTGCAAGGAATTTGATGAAGAAACGTGATGGTAGATTATTGACTGTAGAAGAAGATGGTTTACTGTGGAGGCCAAATTCTTCTTGA

Protein sequence

>Msa0169410

MGFPKKPLFLLLFFIFFTTHFSKSSSSPDYTTLVYKGCSKETFTDPNGAYSQSLSALFGSLVSQSTKTRFFKTTTGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEVAGFPQVSGMQILFKTCGTTNAAGRGFEERRDTAFSVMENGVVSNHGFYVTTYQALYVMGQCEGDVGDSDCGECVKSAVQRAQVECGSSISGQIYLHKCFISFSYYPNGVPKGGHSSSGGGSSSSGYSSSFGGQNTGKTVAIILGGIAAVAFLVICLLFARNLMKKRDGRLLTVEEDGLLWRPNSS*