AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0804130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0804130 MtrunA17_Chr5g0412131 77.880 217 22 1 1 191 1 217 3.00e-115 339
Msa0804130 MtrunA17_Chr5g0412101 74.771 218 28 2 1 191 1 218 1.03e-106 318
Msa0804130 MtrunA17_Chr5g0412111 64.783 230 50 4 10 208 3 232 1.65e-91 278
Msa0804130 MtrunA17_Chr3g0118671 30.453 243 114 10 12 208 6 239 1.08e-17 82.4
Msa0804130 MtrunA17_Chr3g0118701 31.169 231 106 10 11 196 14 236 2.14e-16 79.0
Msa0804130 MtrunA17_Chr3g0118691 29.680 219 112 7 11 191 14 228 2.20e-16 78.6
Msa0804130 MtrunA17_Chr5g0426411 33.775 151 86 5 1 142 1 146 2.27e-15 72.4
Msa0804130 MtrunA17_Chr3g0118751 30.622 209 101 10 11 180 5 208 2.43e-15 75.5
Msa0804130 MtrunA17_Chr3g0118711 29.536 237 116 9 1 194 1 229 2.99e-15 75.5
Msa0804130 MtrunA17_Chr3g0118771 29.091 220 90 9 11 180 20 223 1.85e-14 73.2
Msa0804130 MtrunA17_Chr2g0278711 30.144 209 105 7 11 183 10 213 1.17e-13 70.5
Msa0804130 MtrunA17_Chr3g0118661 29.365 252 107 13 11 208 12 246 1.18e-13 70.5
Msa0804130 MtrunA17_Chr4g0050341 28.571 217 105 11 7 180 14 223 2.42e-13 69.7
Msa0804130 MtrunA17_Chr6g0454191 28.049 246 124 12 11 208 9 249 3.77e-13 69.3
Msa0804130 MtrunA17_Chr6g0454251 25.792 221 107 8 11 183 6 217 2.77e-12 66.2
Msa0804130 MtrunA17_Chr3g0092641 28.241 216 104 10 8 180 8 215 1.14e-11 64.7
Msa0804130 MtrunA17_Chr6g0454181 25.911 247 133 11 8 208 6 248 1.43e-11 64.3
Msa0804130 MtrunA17_Chr5g0426431 28.829 222 95 10 8 180 8 215 2.27e-11 63.9
Msa0804130 MtrunA17_Chr5g0423981 27.193 228 119 10 8 208 6 213 2.62e-11 63.5
Msa0804130 MtrunA17_Chr6g0453951 25.926 216 115 6 7 180 52 264 4.16e-11 63.2
Msa0804130 MtrunA17_Chr3g0118741 54.348 46 21 0 10 55 4 49 4.47e-11 57.8
Msa0804130 MtrunA17_Chr6g0454301 43.284 67 36 1 11 77 262 326 7.99e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0804130 AT1G22380.1 30.374 214 101 8 11 183 12 218 2.44e-18 84.7
Msa0804130 AT1G22340.1 29.524 210 99 10 11 179 12 213 4.18e-16 78.2
Msa0804130 AT1G22370.2 29.665 209 95 9 11 179 12 208 6.64e-16 77.8
Msa0804130 AT1G22360.1 27.700 213 97 7 12 179 10 210 1.01e-13 71.2
Msa0804130 AT1G22360.2 27.700 213 97 7 12 179 10 210 1.04e-13 71.2
Msa0804130 AT3G02100.1 33.784 148 81 5 6 142 7 148 6.93e-13 68.6
Msa0804130 AT1G22400.1 24.651 215 104 8 11 179 12 214 9.39e-13 68.6

Find 49 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATAAGTTGCATTATTT+TGG 0.129986 5_3:+19701923 Msa0804130:CDS
TCTTCCAAGTTTCCGTAAAA+TGG 0.190982 5_3:+19702699 Msa0804130:CDS
ACTGATTTGGCTGCTGAGTT+TGG 0.266869 5_3:+19701969 Msa0804130:CDS
GAGGAGAAGCATCCTGGATT+TGG 0.312050 5_3:+19701825 Msa0804130:CDS
CTGATTTGGCTGCTGAGTTT+GGG 0.334780 5_3:+19701970 Msa0804130:CDS
TTCTAACCCAACAATTAATA+AGG 0.343848 5_3:+19702755 Msa0804130:CDS
AAGTACAACCTTGTCTAATA+TGG 0.344130 5_3:-19702734 None:intergenic
TGGTGATCTTGCTACTGATT+TGG 0.352180 5_3:+19701956 Msa0804130:CDS
CACAGTGAGGAGAAGCATCC+TGG 0.364154 5_3:+19701819 Msa0804130:CDS
AGGGCCAATGGTGTAGAGTC+TGG 0.370603 5_3:-19702849 None:intergenic
TGCGTTGCCTTATTAATTGT+TGG 0.374072 5_3:-19702762 None:intergenic
CACTTCGAAGAAATTGCTCT+TGG 0.405842 5_3:-19702921 None:intergenic
GCGTTGCCTTATTAATTGTT+GGG 0.407863 5_3:-19702761 None:intergenic
AGACGAATTTGGGAGAGAAC+TGG 0.419705 5_3:-19702818 None:intergenic
ATGGTCTTGAATTGTAGCTT+AGG 0.425350 5_3:-19701786 None:intergenic
CTTCCAAGTTTCCGTAAAAT+GGG 0.426008 5_3:+19702700 Msa0804130:CDS
TTCGAAGAAATTGCTCTTGG+AGG 0.443260 5_3:-19702918 None:intergenic
AGCTCAGCTAGCTTTAGCAT+TGG 0.453685 5_3:-19701660 None:intergenic
TCAGTTGTATATGTCAGCTT+TGG 0.454013 5_3:+19702986 Msa0804130:CDS
GGGCCAATGGTGTAGAGTCT+GGG 0.463341 5_3:-19702848 None:intergenic
GACGAATTTGGGAGAGAACT+GGG 0.472716 5_3:-19702817 None:intergenic
AGCATCCTGGATTTGGAGAA+AGG 0.475413 5_3:+19701832 Msa0804130:CDS
GGATTTACATGGCCTTGTGC+TGG 0.486767 5_3:-19701639 None:intergenic
TGGTCTTGAATTGTAGCTTA+GGG 0.493157 5_3:-19701785 None:intergenic
GCTTTAGCATTGGATTTACA+TGG 0.505828 5_3:-19701650 None:intergenic
CAAGAGCAATTTCTTCGAAG+TGG 0.519005 5_3:+19702922 Msa0804130:CDS
GCTATCCTTTCTCCAAATCC+AGG 0.521331 5_3:-19701837 None:intergenic
CATAAGAAACGTGCCAGGCA+TGG 0.531082 5_3:+19702654 Msa0804130:CDS
ACATGGCCTTGTGCTGGACA+TGG 0.534043 5_3:-19701633 None:intergenic
TGAAACTAATGAGACGGTTG+TGG 0.551286 5_3:-19701731 None:intergenic
GTATTGAGGTGGTGGTGAAG+AGG 0.553325 5_3:-19702869 None:intergenic
CATGGGAAGATTAGCAAATG+TGG 0.557319 5_3:-19701615 None:intergenic
GGTTTAATTTATAGGAGATG+AGG 0.559641 5_3:+19702618 Msa0804130:intron
CGCATCATAAGAAACGTGCC+AGG 0.561151 5_3:+19702649 Msa0804130:CDS
TATTGAGGTGGTGGTGAAGA+GGG 0.568969 5_3:-19702868 None:intergenic
GTGGTGGTGAAGAGGGCCAA+TGG 0.570422 5_3:-19702861 None:intergenic
CTCACTGTGAAAATCAGAGA+TGG 0.579959 5_3:-19701805 None:intergenic
GGCCAATGGTGTAGAGTCTG+GGG 0.580965 5_3:-19702847 None:intergenic
CATGGCCTTGTGCTGGACAT+GGG 0.589394 5_3:-19701632 None:intergenic
ACAATAATATCTTTCAACAA+AGG 0.592002 5_3:-19701894 None:intergenic
CGAAGTGGACAGAACTTGCA+TGG 0.592196 5_3:+19702937 Msa0804130:CDS
TATCATTGAAACTAATGAGA+CGG 0.592738 5_3:-19701737 None:intergenic
ATCTTCCCATGTCCAGCACA+AGG 0.604045 5_3:+19701627 Msa0804130:CDS
ATTTATAGGAGATGAGGACA+TGG 0.611104 5_3:+19702624 Msa0804130:intron
ACGAATTTGGGAGAGAACTG+GGG 0.620956 5_3:-19702816 None:intergenic
TGGACAGAACTTGCATGGTG+TGG 0.671150 5_3:+19702942 Msa0804130:CDS
TGTGGATGTACTCAGTGTTG+AGG 0.692218 5_3:-19701713 None:intergenic
GCCAATGGTGTAGAGTCTGG+GGG 0.692520 5_3:-19702846 None:intergenic
TAATGATACCATATTAGACA+AGG 0.701143 5_3:+19702726 Msa0804130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAACTAACAAAAATA+CGG + chr5_3:19702148-19702167 Msa0804130:intron 10.0%
!!! TTTTTTTTTTTTGTAACTTT+TGG + chr5_3:19702366-19702385 Msa0804130:intron 10.0%
!! ATATCTAACTATTTAGTTAA+AGG - chr5_3:19702114-19702133 None:intergenic 15.0%
!!! GTATTTTTGTTAGTTTAATT+AGG - chr5_3:19702149-19702168 None:intergenic 15.0%
!! ACAATAATATCTTTCAACAA+AGG - chr5_3:19701897-19701916 None:intergenic 20.0%
!!! GAAAATAAGTTGCATTATTT+TGG + chr5_3:19701923-19701942 Msa0804130:CDS 20.0%
!!! ATTATTTTGGATGGAATATT+TGG + chr5_3:19701936-19701955 Msa0804130:CDS 20.0%
!! TGAATTATTTCATCTTATCA+TGG + chr5_3:19702055-19702074 Msa0804130:intron 20.0%
! TATCATTGAAACTAATGAGA+CGG - chr5_3:19701740-19701759 None:intergenic 25.0%
!! ATTTTATCACTGAGTTTGTA+TGG + chr5_3:19701867-19701886 Msa0804130:CDS 25.0%
!!! ATAAGTTGCATTATTTTGGA+TGG + chr5_3:19701927-19701946 Msa0804130:CDS 25.0%
! AGTTAAAGGAAAAAGTAAAG+AGG - chr5_3:19702100-19702119 None:intergenic 25.0%
! ACACATAAGTAGATAAGTTA+GGG - chr5_3:19702182-19702201 None:intergenic 25.0%
! ATGCAACATTTGAATTAACT+TGG + chr5_3:19702283-19702302 Msa0804130:intron 25.0%
!!! TAACTTGGTATGTTTGTTTT+AGG + chr5_3:19702298-19702317 Msa0804130:intron 25.0%
! TATGAAACACTTCTAATAGA+AGG + chr5_3:19702467-19702486 Msa0804130:intron 25.0%
!!! AGTGTTCTGGTTTAATTTAT+AGG + chr5_3:19702610-19702629 Msa0804130:intron 25.0%
!! TATCATTATCACCCATTTTA+CGG - chr5_3:19702714-19702733 None:intergenic 25.0%
! TAATGATACCATATTAGACA+AGG + chr5_3:19702726-19702745 Msa0804130:CDS 25.0%
! TTCTAACCCAACAATTAATA+AGG + chr5_3:19702755-19702774 Msa0804130:CDS 25.0%
!!! ATGTTTTTTTTGTGGTATTG+AGG - chr5_3:19702886-19702905 None:intergenic 25.0%
!!! TGATGATGATGTTTTTTTTG+TGG - chr5_3:19702894-19702913 None:intergenic 25.0%
!!! ACATATACAACTGATTTTGA+TGG - chr5_3:19702980-19702999 None:intergenic 25.0%
GAAAAAGTAAAGAGGTAAAG+AGG - chr5_3:19702092-19702111 None:intergenic 30.0%
CACACATAAGTAGATAAGTT+AGG - chr5_3:19702183-19702202 None:intergenic 30.0%
ATGAAAAACTTCTAACAGTC+AGG + chr5_3:19702401-19702420 Msa0804130:intron 30.0%
! AGATGAATTTTGCTACACAT+TGG + chr5_3:19702433-19702452 Msa0804130:intron 30.0%
TATGATCCATATAGAGTTGA+TGG + chr5_3:19702513-19702532 Msa0804130:intron 30.0%
AATGAGTTATATGAGTGTTC+TGG + chr5_3:19702597-19702616 Msa0804130:intron 30.0%
! GGTTTAATTTATAGGAGATG+AGG + chr5_3:19702618-19702637 Msa0804130:intron 30.0%
AAGTACAACCTTGTCTAATA+TGG - chr5_3:19702737-19702756 None:intergenic 30.0%
!!! TTTTTTTTGTGGTATTGAGG+TGG - chr5_3:19702883-19702902 None:intergenic 30.0%
! TGTGGAGAAGATTTTTCCTT+AGG - chr5_3:19701600-19701619 None:intergenic 35.0%
! GCTTTAGCATTGGATTTACA+TGG - chr5_3:19701653-19701672 None:intergenic 35.0%
! TGGTCTTGAATTGTAGCTTA+GGG - chr5_3:19701788-19701807 None:intergenic 35.0%
! ATGGTCTTGAATTGTAGCTT+AGG - chr5_3:19701789-19701808 None:intergenic 35.0%
TGACTATTGTCATCTAGTCT+AGG + chr5_3:19702210-19702229 Msa0804130:intron 35.0%
TTCTCACCATCAACTCTATA+TGG - chr5_3:19702522-19702541 None:intergenic 35.0%
ATTTATAGGAGATGAGGACA+TGG + chr5_3:19702624-19702643 Msa0804130:intron 35.0%
TCTTCCAAGTTTCCGTAAAA+TGG + chr5_3:19702699-19702718 Msa0804130:CDS 35.0%
CTTCCAAGTTTCCGTAAAAT+GGG + chr5_3:19702700-19702719 Msa0804130:CDS 35.0%
! GCGTTGCCTTATTAATTGTT+GGG - chr5_3:19702764-19702783 None:intergenic 35.0%
! TGCGTTGCCTTATTAATTGT+TGG - chr5_3:19702765-19702784 None:intergenic 35.0%
TCAGTTGTATATGTCAGCTT+TGG + chr5_3:19702986-19703005 Msa0804130:CDS 35.0%
CATGGGAAGATTAGCAAATG+TGG - chr5_3:19701618-19701637 None:intergenic 40.0%
TGAAACTAATGAGACGGTTG+TGG - chr5_3:19701734-19701753 None:intergenic 40.0%
CTCACTGTGAAAATCAGAGA+TGG - chr5_3:19701808-19701827 None:intergenic 40.0%
! CATCTCTGATTTTCACAGTG+AGG + chr5_3:19701806-19701825 Msa0804130:CDS 40.0%
! TGGTGATCTTGCTACTGATT+TGG + chr5_3:19701956-19701975 Msa0804130:CDS 40.0%
! TCACCCATTTTACGGAAACT+TGG - chr5_3:19702706-19702725 None:intergenic 40.0%
!! TTTTTGTGGTATTGAGGTGG+TGG - chr5_3:19702880-19702899 None:intergenic 40.0%
TTCGAAGAAATTGCTCTTGG+AGG - chr5_3:19702921-19702940 None:intergenic 40.0%
CACTTCGAAGAAATTGCTCT+TGG - chr5_3:19702924-19702943 None:intergenic 40.0%
CAAGAGCAATTTCTTCGAAG+TGG + chr5_3:19702922-19702941 Msa0804130:CDS 40.0%
!! AGCTCAGCTAGCTTTAGCAT+TGG - chr5_3:19701663-19701682 None:intergenic 45.0%
TGTGGATGTACTCAGTGTTG+AGG - chr5_3:19701716-19701735 None:intergenic 45.0%
AGCATCCTGGATTTGGAGAA+AGG + chr5_3:19701832-19701851 Msa0804130:CDS 45.0%
GCTATCCTTTCTCCAAATCC+AGG - chr5_3:19701840-19701859 None:intergenic 45.0%
!! ACTGATTTGGCTGCTGAGTT+TGG + chr5_3:19701969-19701988 Msa0804130:CDS 45.0%
!! CTGATTTGGCTGCTGAGTTT+GGG + chr5_3:19701970-19701989 Msa0804130:CDS 45.0%
ACGAATTTGGGAGAGAACTG+GGG - chr5_3:19702819-19702838 None:intergenic 45.0%
GACGAATTTGGGAGAGAACT+GGG - chr5_3:19702820-19702839 None:intergenic 45.0%
AGACGAATTTGGGAGAGAAC+TGG - chr5_3:19702821-19702840 None:intergenic 45.0%
! TATTGAGGTGGTGGTGAAGA+GGG - chr5_3:19702871-19702890 None:intergenic 45.0%
ATCTTCCCATGTCCAGCACA+AGG + chr5_3:19701627-19701646 Msa0804130:CDS 50.0%
!! GGATTTACATGGCCTTGTGC+TGG - chr5_3:19701642-19701661 None:intergenic 50.0%
!! GAGGAGAAGCATCCTGGATT+TGG + chr5_3:19701825-19701844 Msa0804130:CDS 50.0%
CGCATCATAAGAAACGTGCC+AGG + chr5_3:19702649-19702668 Msa0804130:CDS 50.0%
CATAAGAAACGTGCCAGGCA+TGG + chr5_3:19702654-19702673 Msa0804130:CDS 50.0%
! CGAAGCAAGTTTTCCATGCC+TGG - chr5_3:19702670-19702689 None:intergenic 50.0%
! GGGGGCATGAAGACGAATTT+GGG - chr5_3:19702831-19702850 None:intergenic 50.0%
! TGGGGGCATGAAGACGAATT+TGG - chr5_3:19702832-19702851 None:intergenic 50.0%
! GTATTGAGGTGGTGGTGAAG+AGG - chr5_3:19702872-19702891 None:intergenic 50.0%
CGAAGTGGACAGAACTTGCA+TGG + chr5_3:19702937-19702956 Msa0804130:CDS 50.0%
TGGACAGAACTTGCATGGTG+TGG + chr5_3:19702942-19702961 Msa0804130:CDS 50.0%
!! CATGGCCTTGTGCTGGACAT+GGG - chr5_3:19701635-19701654 None:intergenic 55.0%
!! ACATGGCCTTGTGCTGGACA+TGG - chr5_3:19701636-19701655 None:intergenic 55.0%
!! CACAGTGAGGAGAAGCATCC+TGG + chr5_3:19701819-19701838 Msa0804130:CDS 55.0%
GCCAATGGTGTAGAGTCTGG+GGG - chr5_3:19702849-19702868 None:intergenic 55.0%
GGCCAATGGTGTAGAGTCTG+GGG - chr5_3:19702850-19702869 None:intergenic 55.0%
GGGCCAATGGTGTAGAGTCT+GGG - chr5_3:19702851-19702870 None:intergenic 55.0%
AGGGCCAATGGTGTAGAGTC+TGG - chr5_3:19702852-19702871 None:intergenic 55.0%
GCCCCCAGACTCTACACCAT+TGG + chr5_3:19702845-19702864 Msa0804130:CDS 60.0%
! GTGGTGGTGAAGAGGGCCAA+TGG - chr5_3:19702864-19702883 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 19701585 19703044 19701585 ID=Msa0804130;Name=Msa0804130
chr5_3 mRNA 19701585 19703044 19701585 ID=Msa0804130-mRNA-1;Parent=Msa0804130;Name=Msa0804130-mRNA-1;_AED=0.42;_eAED=0.57;_QI=0|0|0|1|1|1|2|0|279
chr5_3 exon 19701585 19702011 19701585 ID=Msa0804130-mRNA-1:exon:8082;Parent=Msa0804130-mRNA-1
chr5_3 exon 19702632 19703044 19702632 ID=Msa0804130-mRNA-1:exon:8083;Parent=Msa0804130-mRNA-1
chr5_3 CDS 19701585 19702011 19701585 ID=Msa0804130-mRNA-1:cds;Parent=Msa0804130-mRNA-1
chr5_3 CDS 19702632 19703044 19702632 ID=Msa0804130-mRNA-1:cds;Parent=Msa0804130-mRNA-1
Gene Sequence

>Msa0804130

ATGGAAACACAGCCTAAGGAAAAATCTTCTCCACATTTGCTAATCTTCCCATGTCCAGCACAAGGCCATGTAAATCCAATGCTAAAGCTAGCTGAGCTTCTTGCAATTCAAAATCTCAACATAACTTTCCTCAACACTGAGTACATCCACAACCGTCTCATTAGTTTCAATGATAATATTCAATCTCTTTCAGAATGTTACCCTAAGCTACAATTCAAGACCATCTCTGATTTTCACAGTGAGGAGAAGCATCCTGGATTTGGAGAAAGGATAGCAGATGTAATTTTATCACTGAGTTTGTATGGTAAGCCTTTGTTGAAAGATATTATTGTGTCTGAGAAAATAAGTTGCATTATTTTGGATGGAATATTTGGTGATCTTGCTACTGATTTGGCTGCTGAGTTTGGGATACAATTGATTCATTTTCGAGATGAGGACATGGATCGCATCATAAGAAACGTGCCAGGCATGGAAAACTTGCTTCGATGTAGAGATCTTCCAAGTTTCCGTAAAATGGGTGATAATGATACCATATTAGACAAGGTTGTACTTCTAACCCAACAATTAATAAGGCAACGCATTAATACTCAACACGTTCGAGAATCTAGAATCCCCAGTTCTCTCCCAAATTCGTCTTCATGCCCCCAGACTCTACACCATTGGCCCTCTTCACCACCACCTCAATACCACAAAAAAAACATCATCATCATCATCCTCCAAGAGCAATTTCTTCGAAGTGGACAGAACTTGCATGGTGTGGCTCGATTCTCAACCATCAAAATCAGTTGTATATGTCAGCTTTGGCAGCATCACGACAATGACAAGAGAAGAAATACTTGA

Protein sequence

>Msa0804130

METQPKEKSSPHLLIFPCPAQGHVNPMLKLAELLAIQNLNITFLNTEYIHNRLISFNDNIQSLSECYPKLQFKTISDFHSEEKHPGFGERIADVILSLSLYGKPLLKDIIVSEKISCIILDGIFGDLATDLAAEFGIQLIHFRDEDMDRIIRNVPGMENLLRCRDLPSFRKMGDNDTILDKVVLLTQQLIRQRINTQHVRESRIPSSLPNSSSCPQTLHHWPSSPPPQYHKKNIIIIILQEQFLRSGQNLHGVARFSTIKISCICQLWQHHDNDKRRNT*