AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0217470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217470 MtrunA17_Chr2g0319281 98.765 81 1 0 17 97 112 192 4.78e-53 165
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217470 AT3G01440.1 68.750 80 25 0 18 97 109 188 3.62e-35 119

Find 35 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTACTTGTCCTGTCTTAA+AGG 0.141076 2_1:+64301963 None:intergenic
TAGCTAAGGATAATGGATTT+TGG 0.177277 2_1:-64301813 Msa0217470:exon
TTTGTTTCTTTAGTTCTTAC+AGG 0.230480 2_1:-64301859 Msa0217470:five_prime_UTR
CAAGTAGTAGTTGGATTTCT+TGG 0.273988 2_1:-64301949 Msa0217470:five_prime_UTR
TTAAAGGTTCTTTGTGCATT+TGG 0.275452 2_1:+64301979 None:intergenic
GAGTAGCTTCTTCACCATTA+TGG 0.285901 2_1:-64302061 Msa0217470:five_prime_UTR
AAACTTTACATGGCGAATAT+TGG 0.290067 2_1:-64300935 Msa0217470:CDS
TTTGGAACAAGGCATGTAAT+TGG 0.297323 2_1:+64301997 None:intergenic
CTATTAGTGACAGTTGGTTC+TGG 0.310416 2_1:+64301775 None:intergenic
TGAAAGTTGACTTGAGTCTT+AGG 0.313305 2_1:+64300813 None:intergenic
GTGACAGTTGGTTCTGGAAT+AGG 0.317646 2_1:+64301781 None:intergenic
ATTTCACTAGCTAAGGATAA+TGG 0.333626 2_1:-64301820 Msa0217470:exon
TGTAATTGGAGTTAAAGTTT+TGG 0.348907 2_1:+64302011 None:intergenic
ACTCCATGCAAATTTGTTAG+AGG 0.418986 2_1:+64302036 None:intergenic
AAGACACACTCAACTATGTT+AGG 0.422431 2_1:-64300841 Msa0217470:CDS
ATGGCGAATATTGGAGCAAA+AGG 0.440394 2_1:-64300926 Msa0217470:CDS
ATTTGTTAGAGGAGCCATAA+TGG 0.451258 2_1:+64302047 None:intergenic
AATTGCTGCTTATCTTCAGC+AGG 0.453994 2_1:+64300755 None:intergenic
AAGACAGGACAAGTAGTAGT+TGG 0.458371 2_1:-64301958 Msa0217470:five_prime_UTR
ATCTTACTATTAGTGACAGT+TGG 0.488173 2_1:+64301769 None:intergenic
TTCTTTGTGCATTTGGAACA+AGG 0.495526 2_1:+64301986 None:intergenic
TAGCACTAGCACTTCCCAAA+TGG 0.498901 2_1:-64302157 Msa0217470:five_prime_UTR
TGCATTTGATCTTCTTGCAA+TGG 0.504301 2_1:-64300879 Msa0217470:CDS
TCCACTGAGTGGCACTAGAA+AGG 0.513605 2_1:+64302185 None:intergenic
TGAGAAGATTTCACTAGCTA+AGG 0.517657 2_1:-64301827 Msa0217470:five_prime_UTR
TATTAAGAAGAAACTTTACA+TGG 0.528232 2_1:-64300945 Msa0217470:intron
ATTTGATCTTCTTGCAATGG+CGG 0.551344 2_1:-64300876 Msa0217470:CDS
TTTGGGAAGTGCTAGTGCTA+TGG 0.552026 2_1:+64302160 None:intergenic
GCACAAAGAACCTTTAAGAC+AGG 0.560597 2_1:-64301973 Msa0217470:five_prime_UTR
GCTCCTCTAACAAATTTGCA+TGG 0.591211 2_1:-64302039 Msa0217470:five_prime_UTR
ACACACTCAACTATGTTAGG+AGG 0.598523 2_1:-64300838 Msa0217470:CDS
TAAGCAGCAATTGACTGATG+TGG 0.624593 2_1:-64300744 Msa0217470:CDS
TCCTTTCTAGTGCCACTCAG+TGG 0.641696 2_1:-64302186 Msa0217470:five_prime_UTR
GTGCTATGGTGTCCACTGAG+TGG 0.645765 2_1:+64302174 None:intergenic
ATTGCTGCTTATCTTCAGCA+GGG 0.656002 2_1:+64300756 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGATAATTCATTAATTTTT+TGG + chr2_1:64300790-64300809 None:intergenic 10.0%
!! TTAATAATAAACAAATGTAA+TGG + chr2_1:64301562-64301581 None:intergenic 10.0%
!! ATATTCTATTATTCAAATTT+AGG + chr2_1:64301760-64301779 None:intergenic 10.0%
!!! TAGATAATTCATTAATTTTT+TGG + chr2_1:64300790-64300809 None:intergenic 10.0%
!! TTAATAATAAACAAATGTAA+TGG + chr2_1:64301562-64301581 None:intergenic 10.0%
!! ATATTCTATTATTCAAATTT+AGG + chr2_1:64301760-64301779 None:intergenic 10.0%
!! AAATATTAATTGTACTATCA+TGG - chr2_1:64301360-64301379 Msa0217470:intron 15.0%
!! TTTGTTTATTATTAAATCGA+TGG + chr2_1:64301567-64301586 None:intergenic 15.0%
!! TTAATAATAAACAAATGCAA+TGG - chr2_1:64301473-64301492 Msa0217470:intron 15.0%
!! TTTGTTTATTATTAAATCGA+TGG - chr2_1:64301567-64301586 Msa0217470:intron 15.0%
!! AAATATTAATTGTACTATCA+TGG - chr2_1:64301360-64301379 Msa0217470:intron 15.0%
!! TTTGTTTATTATTAAATCGA+TGG + chr2_1:64301567-64301586 None:intergenic 15.0%
!! TTAATAATAAACAAATGCAA+TGG - chr2_1:64301473-64301492 Msa0217470:intron 15.0%
!! TTTGTTTATTATTAAATCGA+TGG - chr2_1:64301567-64301586 Msa0217470:intron 15.0%
!! TATAGCTAATGAATTTACAT+TGG - chr2_1:64301163-64301182 Msa0217470:intron 20.0%
!!! TTTTATGTTTCAATTGACAT+TGG - chr2_1:64301253-64301272 Msa0217470:intron 20.0%
!! TTTATGTTTCAATTGACATT+GGG - chr2_1:64301254-64301273 Msa0217470:intron 20.0%
!! TATAATTAACTTCATCATGA+TGG + chr2_1:64301591-64301610 None:intergenic 20.0%
!! TATAATTAACTTCATCATGA+TGG - chr2_1:64301591-64301610 Msa0217470:intron 20.0%
!! TATTAAGAAGAAACTTTACA+TGG - chr2_1:64301954-64301973 Msa0217470:five_prime_UTR 20.0%
!! TATAGCTAATGAATTTACAT+TGG - chr2_1:64301163-64301182 Msa0217470:intron 20.0%
!!! TTTTATGTTTCAATTGACAT+TGG - chr2_1:64301253-64301272 Msa0217470:intron 20.0%
!! TTTATGTTTCAATTGACATT+GGG - chr2_1:64301254-64301273 Msa0217470:intron 20.0%
!! TATAATTAACTTCATCATGA+TGG + chr2_1:64301591-64301610 None:intergenic 20.0%
!! TATAATTAACTTCATCATGA+TGG - chr2_1:64301591-64301610 Msa0217470:intron 20.0%
!! TATTAAGAAGAAACTTTACA+TGG - chr2_1:64301954-64301973 Msa0217470:five_prime_UTR 20.0%
!! TTTTTGTGACATATCCATTT+GGG + chr2_1:64300759-64300778 None:intergenic 25.0%
!!! TTTTTTGTGACATATCCATT+TGG + chr2_1:64300760-64300779 None:intergenic 25.0%
!! TGTAATTGGAGTTAAAGTTT+TGG + chr2_1:64300891-64300910 None:intergenic 25.0%
!!! TTTGTTTCTTTAGTTCTTAC+AGG - chr2_1:64301040-64301059 Msa0217470:intron 25.0%
! GTTATGATTGAAATTTAGCA+TGG - chr2_1:64301328-64301347 Msa0217470:intron 25.0%
! CAATGTGAATGATGATTATA+TGG - chr2_1:64301893-64301912 Msa0217470:five_prime_UTR 25.0%
!! TTTTTGTGACATATCCATTT+GGG + chr2_1:64300759-64300778 None:intergenic 25.0%
!!! TTTTTTGTGACATATCCATT+TGG + chr2_1:64300760-64300779 None:intergenic 25.0%
!! TGTAATTGGAGTTAAAGTTT+TGG + chr2_1:64300891-64300910 None:intergenic 25.0%
!!! TTTGTTTCTTTAGTTCTTAC+AGG - chr2_1:64301040-64301059 Msa0217470:intron 25.0%
! GTTATGATTGAAATTTAGCA+TGG - chr2_1:64301328-64301347 Msa0217470:intron 25.0%
! CAATGTGAATGATGATTATA+TGG - chr2_1:64301893-64301912 Msa0217470:five_prime_UTR 25.0%
TTAAAGGTTCTTTGTGCATT+TGG + chr2_1:64300923-64300942 None:intergenic 30.0%
ATTTCACTAGCTAAGGATAA+TGG - chr2_1:64301079-64301098 Msa0217470:intron 30.0%
!!! TAGCTAAGGATAATGGATTT+TGG - chr2_1:64301086-64301105 Msa0217470:intron 30.0%
!!! GATAATGGATTTTGGATTGA+TGG - chr2_1:64301094-64301113 Msa0217470:intron 30.0%
ATCTTACTATTAGTGACAGT+TGG + chr2_1:64301133-64301152 None:intergenic 30.0%
CAAATCTTGTACATAAGCAA+TGG - chr2_1:64301399-64301418 Msa0217470:intron 30.0%
TACCTATTAGTGAAGTACAA+TGG - chr2_1:64301514-64301533 Msa0217470:intron 30.0%
TTAGGGGTAATCTTGTAAAT+TGG - chr2_1:64301700-64301719 Msa0217470:intron 30.0%
! CAGTATTTTATCACAGAGTT+CGG + chr2_1:64301813-64301832 None:intergenic 30.0%
!! TATCTGTTTACTTTTGAACC+AGG - chr2_1:64301857-64301876 Msa0217470:five_prime_UTR 30.0%
!! ATCTGTTTACTTTTGAACCA+GGG - chr2_1:64301858-64301877 Msa0217470:five_prime_UTR 30.0%
AGATATTGCGAATGAGAAAA+CGG - chr2_1:64301918-64301937 Msa0217470:five_prime_UTR 30.0%
AAACTTTACATGGCGAATAT+TGG - chr2_1:64301964-64301983 Msa0217470:five_prime_UTR 30.0%
!! CATGTACTTTGATTTTGACA+AGG - chr2_1:64302107-64302126 Msa0217470:five_prime_UTR 30.0%
TTAAAGGTTCTTTGTGCATT+TGG + chr2_1:64300923-64300942 None:intergenic 30.0%
ATTTCACTAGCTAAGGATAA+TGG - chr2_1:64301079-64301098 Msa0217470:intron 30.0%
!!! TAGCTAAGGATAATGGATTT+TGG - chr2_1:64301086-64301105 Msa0217470:intron 30.0%
!!! GATAATGGATTTTGGATTGA+TGG - chr2_1:64301094-64301113 Msa0217470:intron 30.0%
ATCTTACTATTAGTGACAGT+TGG + chr2_1:64301133-64301152 None:intergenic 30.0%
CAAATCTTGTACATAAGCAA+TGG - chr2_1:64301399-64301418 Msa0217470:intron 30.0%
TACCTATTAGTGAAGTACAA+TGG - chr2_1:64301514-64301533 Msa0217470:intron 30.0%
TTAGGGGTAATCTTGTAAAT+TGG - chr2_1:64301700-64301719 Msa0217470:intron 30.0%
! CAGTATTTTATCACAGAGTT+CGG + chr2_1:64301813-64301832 None:intergenic 30.0%
!! TATCTGTTTACTTTTGAACC+AGG - chr2_1:64301857-64301876 Msa0217470:five_prime_UTR 30.0%
!! ATCTGTTTACTTTTGAACCA+GGG - chr2_1:64301858-64301877 Msa0217470:five_prime_UTR 30.0%
AGATATTGCGAATGAGAAAA+CGG - chr2_1:64301918-64301937 Msa0217470:five_prime_UTR 30.0%
AAACTTTACATGGCGAATAT+TGG - chr2_1:64301964-64301983 Msa0217470:five_prime_UTR 30.0%
!! CATGTACTTTGATTTTGACA+AGG - chr2_1:64302107-64302126 Msa0217470:five_prime_UTR 30.0%
! ATTTGTTAGAGGAGCCATAA+TGG + chr2_1:64300855-64300874 None:intergenic 35.0%
!! ACTCCATGCAAATTTGTTAG+AGG + chr2_1:64300866-64300885 None:intergenic 35.0%
! TTTGGAACAAGGCATGTAAT+TGG + chr2_1:64300905-64300924 None:intergenic 35.0%
! TTCTTTGTGCATTTGGAACA+AGG + chr2_1:64300916-64300935 None:intergenic 35.0%
TACTACTTGTCCTGTCTTAA+AGG + chr2_1:64300939-64300958 None:intergenic 35.0%
CAAGTAGTAGTTGGATTTCT+TGG - chr2_1:64300950-64300969 Msa0217470:intron 35.0%
TGAGAAGATTTCACTAGCTA+AGG - chr2_1:64301072-64301091 Msa0217470:intron 35.0%
GACATTGGGACAATTATGAT+TGG - chr2_1:64301268-64301287 Msa0217470:intron 35.0%
TGCCATTGTACTTCACTAAT+AGG + chr2_1:64301519-64301538 None:intergenic 35.0%
GATATTGCGAATGAGAAAAC+GGG - chr2_1:64301919-64301938 Msa0217470:five_prime_UTR 35.0%
ATATTGCGAATGAGAAAACG+GGG - chr2_1:64301920-64301939 Msa0217470:five_prime_UTR 35.0%
!!! GAGCAAAAGGAAGTGTTTTA+AGG - chr2_1:64301986-64302005 Msa0217470:five_prime_UTR 35.0%
TGCATTTGATCTTCTTGCAA+TGG - chr2_1:64302020-64302039 Msa0217470:five_prime_UTR 35.0%
ATTTGATCTTCTTGCAATGG+CGG - chr2_1:64302023-64302042 Msa0217470:five_prime_UTR 35.0%
AAGACACACTCAACTATGTT+AGG - chr2_1:64302058-64302077 Msa0217470:five_prime_UTR 35.0%
!! TGAAAGTTGACTTGAGTCTT+AGG + chr2_1:64302089-64302108 None:intergenic 35.0%
! ATTTGTTAGAGGAGCCATAA+TGG + chr2_1:64300855-64300874 None:intergenic 35.0%
!! ACTCCATGCAAATTTGTTAG+AGG + chr2_1:64300866-64300885 None:intergenic 35.0%
! TTTGGAACAAGGCATGTAAT+TGG + chr2_1:64300905-64300924 None:intergenic 35.0%
! TTCTTTGTGCATTTGGAACA+AGG + chr2_1:64300916-64300935 None:intergenic 35.0%
TACTACTTGTCCTGTCTTAA+AGG + chr2_1:64300939-64300958 None:intergenic 35.0%
CAAGTAGTAGTTGGATTTCT+TGG - chr2_1:64300950-64300969 Msa0217470:intron 35.0%
TGAGAAGATTTCACTAGCTA+AGG - chr2_1:64301072-64301091 Msa0217470:intron 35.0%
GACATTGGGACAATTATGAT+TGG - chr2_1:64301268-64301287 Msa0217470:intron 35.0%
TGCCATTGTACTTCACTAAT+AGG + chr2_1:64301519-64301538 None:intergenic 35.0%
GATATTGCGAATGAGAAAAC+GGG - chr2_1:64301919-64301938 Msa0217470:five_prime_UTR 35.0%
ATATTGCGAATGAGAAAACG+GGG - chr2_1:64301920-64301939 Msa0217470:five_prime_UTR 35.0%
!!! GAGCAAAAGGAAGTGTTTTA+AGG - chr2_1:64301986-64302005 Msa0217470:five_prime_UTR 35.0%
TGCATTTGATCTTCTTGCAA+TGG - chr2_1:64302020-64302039 Msa0217470:five_prime_UTR 35.0%
ATTTGATCTTCTTGCAATGG+CGG - chr2_1:64302023-64302042 Msa0217470:five_prime_UTR 35.0%
AAGACACACTCAACTATGTT+AGG - chr2_1:64302058-64302077 Msa0217470:five_prime_UTR 35.0%
!! TGAAAGTTGACTTGAGTCTT+AGG + chr2_1:64302089-64302108 None:intergenic 35.0%
GAGTAGCTTCTTCACCATTA+TGG - chr2_1:64300838-64300857 Msa0217470:CDS 40.0%
GCTCCTCTAACAAATTTGCA+TGG - chr2_1:64300860-64300879 Msa0217470:CDS 40.0%
!! GCACAAAGAACCTTTAAGAC+AGG - chr2_1:64300926-64300945 Msa0217470:CDS 40.0%
AAGACAGGACAAGTAGTAGT+TGG - chr2_1:64300941-64300960 Msa0217470:intron 40.0%
! CTATTAGTGACAGTTGGTTC+TGG + chr2_1:64301127-64301146 None:intergenic 40.0%
GACTTGTGCACTTATCAGTA+AGG + chr2_1:64301650-64301669 None:intergenic 40.0%
TAGACCATACTAAGTGGCTT+AGG - chr2_1:64301682-64301701 Msa0217470:intron 40.0%
AGACCATACTAAGTGGCTTA+GGG - chr2_1:64301683-64301702 Msa0217470:intron 40.0%
CATTGTTAGAACAAGAACCC+TGG + chr2_1:64301878-64301897 None:intergenic 40.0%
ATGGCGAATATTGGAGCAAA+AGG - chr2_1:64301973-64301992 Msa0217470:five_prime_UTR 40.0%
ACACACTCAACTATGTTAGG+AGG - chr2_1:64302061-64302080 Msa0217470:five_prime_UTR 40.0%
! ATTGCTGCTTATCTTCAGCA+GGG + chr2_1:64302146-64302165 None:intergenic 40.0%
! AATTGCTGCTTATCTTCAGC+AGG + chr2_1:64302147-64302166 None:intergenic 40.0%
TAAGCAGCAATTGACTGATG+TGG - chr2_1:64302155-64302174 Msa0217470:five_prime_UTR 40.0%
GAGTAGCTTCTTCACCATTA+TGG - chr2_1:64300838-64300857 Msa0217470:CDS 40.0%
GCTCCTCTAACAAATTTGCA+TGG - chr2_1:64300860-64300879 Msa0217470:CDS 40.0%
!! GCACAAAGAACCTTTAAGAC+AGG - chr2_1:64300926-64300945 Msa0217470:CDS 40.0%
AAGACAGGACAAGTAGTAGT+TGG - chr2_1:64300941-64300960 Msa0217470:intron 40.0%
! CTATTAGTGACAGTTGGTTC+TGG + chr2_1:64301127-64301146 None:intergenic 40.0%
GACTTGTGCACTTATCAGTA+AGG + chr2_1:64301650-64301669 None:intergenic 40.0%
TAGACCATACTAAGTGGCTT+AGG - chr2_1:64301682-64301701 Msa0217470:intron 40.0%
AGACCATACTAAGTGGCTTA+GGG - chr2_1:64301683-64301702 Msa0217470:intron 40.0%
CATTGTTAGAACAAGAACCC+TGG + chr2_1:64301878-64301897 None:intergenic 40.0%
ATGGCGAATATTGGAGCAAA+AGG - chr2_1:64301973-64301992 Msa0217470:five_prime_UTR 40.0%
ACACACTCAACTATGTTAGG+AGG - chr2_1:64302061-64302080 Msa0217470:five_prime_UTR 40.0%
! ATTGCTGCTTATCTTCAGCA+GGG + chr2_1:64302146-64302165 None:intergenic 40.0%
! AATTGCTGCTTATCTTCAGC+AGG + chr2_1:64302147-64302166 None:intergenic 40.0%
TAAGCAGCAATTGACTGATG+TGG - chr2_1:64302155-64302174 Msa0217470:five_prime_UTR 40.0%
!! TTTGGGAAGTGCTAGTGCTA+TGG + chr2_1:64300742-64300761 None:intergenic 45.0%
TAGCACTAGCACTTCCCAAA+TGG - chr2_1:64300742-64300761 Msa0217470:CDS 45.0%
!! GTGACAGTTGGTTCTGGAAT+AGG + chr2_1:64301121-64301140 None:intergenic 45.0%
GCTGACTAGACCATACTAAG+TGG - chr2_1:64301676-64301695 Msa0217470:intron 45.0%
GACCATACTAAGTGGCTTAG+GGG - chr2_1:64301684-64301703 Msa0217470:intron 45.0%
TACCCCTAAGCCACTTAGTA+TGG + chr2_1:64301689-64301708 None:intergenic 45.0%
GTCAGAGACGTAGACACAAT+TGG - chr2_1:64301787-64301806 Msa0217470:CDS 45.0%
!! TTTGGGAAGTGCTAGTGCTA+TGG + chr2_1:64300742-64300761 None:intergenic 45.0%
TAGCACTAGCACTTCCCAAA+TGG - chr2_1:64300742-64300761 Msa0217470:CDS 45.0%
!! GTGACAGTTGGTTCTGGAAT+AGG + chr2_1:64301121-64301140 None:intergenic 45.0%
GCTGACTAGACCATACTAAG+TGG - chr2_1:64301676-64301695 Msa0217470:intron 45.0%
GACCATACTAAGTGGCTTAG+GGG - chr2_1:64301684-64301703 Msa0217470:intron 45.0%
TACCCCTAAGCCACTTAGTA+TGG + chr2_1:64301689-64301708 None:intergenic 45.0%
GTCAGAGACGTAGACACAAT+TGG - chr2_1:64301787-64301806 Msa0217470:CDS 45.0%
GTGCTATGGTGTCCACTGAG+TGG + chr2_1:64300728-64300747 None:intergenic 55.0%
GTGCTATGGTGTCCACTGAG+TGG + chr2_1:64300728-64300747 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 64300715 64302206 64300715 ID=Msa0217470;Name=Msa0217470
chr2_1 mRNA 64300715 64302206 64300715 ID=Msa0217470-mRNA-1;Parent=Msa0217470;Name=Msa0217470-mRNA-1;_AED=0.17;_eAED=0.17;_QI=383|0|0.5|1|0|0.5|2|0|97
chr2_1 exon 64300715 64300957 64300715 ID=Msa0217470-mRNA-1:exon:17863;Parent=Msa0217470-mRNA-1
chr2_1 exon 64301776 64302206 64301776 ID=Msa0217470-mRNA-1:exon:17862;Parent=Msa0217470-mRNA-1
chr2_1 five_prime_UTR 64301824 64302206 64301824 ID=Msa0217470-mRNA-1:five_prime_utr;Parent=Msa0217470-mRNA-1
chr2_1 CDS 64301776 64301823 64301776 ID=Msa0217470-mRNA-1:cds;Parent=Msa0217470-mRNA-1
chr2_1 CDS 64300715 64300957 64300715 ID=Msa0217470-mRNA-1:cds;Parent=Msa0217470-mRNA-1
Gene Sequence

>Msa0217470

ATGGATTTTGGATTGATGGACCTATTCCAGAACCAACTGTCACTAATAAAACTTTACATGGCGAATATTGGAGCAAAAGGAAGTGTTTTAAGGATCAAGAAATATGCATTTGATCTTCTTGCAATGGCGGATTTGATTGCACAAGACACACTCAACTATGTTAGGAGGTACCTAAGACTCAAGTCAACTTTCATGTACTTTGATTTTGACAAGGTTATCTCTGCTGTCCCTGCTGAAGATAAGCAGCAATTGACTGATGTGGCAAACAAATTGTTTGACAATTTTGAAAAG

Protein sequence

>Msa0217470

MDFGLMDLFQNQLSLIKLYMANIGAKGSVLRIKKYAFDLLAMADLIAQDTLNYVRRYLRLKSTFMYFDFDKVISAVPAEDKQQLTDVANKLFDNFEK