AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1366000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366000 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366000 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366000 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366000 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366000 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366000 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1366000 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366000 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018442:+95861 Msa1366000:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018442:-95841 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018442:+95830 Msa1366000:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018442:+95530 Msa1366000:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018442:+95586 Msa1366000:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018442:+95889 Msa1366000:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018442:-95801 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018442:+95387 Msa1366000:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018442:-95710 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018442:-95836 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018442:-95655 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018442:+95862 Msa1366000:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018442:+95866 Msa1366000:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018442:-95410 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018442:-95815 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018442:+95551 Msa1366000:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018442:-95654 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018442:-95389 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018442:-95802 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018442:+95730 Msa1366000:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018442:-95728 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018442:-95570 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018442:+95398 Msa1366000:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018442:+95731 Msa1366000:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018442:-95647 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018442:-95820 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018442:-95798 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018442:-95800 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018442:+95330 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018442:+95329 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018442:+95542 Msa1366000:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018442:-95670 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018442:-95799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG + tig0018442:95861-95880 Msa1366000:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG + tig0018442:95862-95881 Msa1366000:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG + tig0018442:95889-95908 Msa1366000:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG + tig0018442:95488-95507 Msa1366000:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG - tig0018442:95573-95592 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG + tig0018442:95608-95627 Msa1366000:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG - tig0018442:95839-95858 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG - tig0018442:95844-95863 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG + tig0018442:95866-95885 Msa1366000:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG + tig0018442:95358-95377 Msa1366000:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG + tig0018442:95359-95378 Msa1366000:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG + tig0018442:95586-95605 Msa1366000:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG + tig0018442:95607-95626 Msa1366000:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG - tig0018442:95673-95692 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG + tig0018442:95671-95690 Msa1366000:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG - tig0018442:95731-95750 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG + tig0018442:95730-95749 Msa1366000:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG + tig0018442:95731-95750 Msa1366000:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG + tig0018442:95830-95849 Msa1366000:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG - tig0018442:95356-95375 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG + tig0018442:95360-95379 Msa1366000:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG + tig0018442:95387-95406 Msa1366000:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG + tig0018442:95613-95632 Msa1366000:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG - tig0018442:95657-95676 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG - tig0018442:95658-95677 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG + tig0018442:95361-95380 Msa1366000:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG - tig0018442:95439-95458 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG + tig0018442:95530-95549 Msa1366000:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG - tig0018442:95650-95669 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG - tig0018442:95713-95732 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG - tig0018442:95805-95824 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG - tig0018442:95818-95837 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG - tig0018442:95823-95842 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG + tig0018442:95398-95417 Msa1366000:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG - tig0018442:95413-95432 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG + tig0018442:95551-95570 Msa1366000:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG - tig0018442:95804-95823 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG - tig0018442:95392-95411 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG + tig0018442:95542-95561 Msa1366000:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG - tig0018442:95803-95822 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG - tig0018442:95801-95820 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG - tig0018442:95802-95821 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG + tig0018442:95371-95390 Msa1366000:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018442 gene 95335 95940 95335 ID=Msa1366000;Name=Msa1366000
tig0018442 mRNA 95335 95940 95335 ID=Msa1366000-mRNA-1;Parent=Msa1366000;Name=Msa1366000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018442 exon 95335 95940 95335 ID=Msa1366000-mRNA-1:exon:14915;Parent=Msa1366000-mRNA-1
tig0018442 CDS 95335 95940 95335 ID=Msa1366000-mRNA-1:cds;Parent=Msa1366000-mRNA-1
Gene Sequence

>Msa1366000

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1366000

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*