Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983720 | YP_009141617.1 | 100.000 | 510 | 0 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1021 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983720 | sp|Q9BBS3|ATPA_LOTJA | 97.255 | 510 | 14 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1001 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983720 | A0A1J0I0I3 | 100.000 | 510 | 0 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1021 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002690 | Msa0983720 | 0.833632 | 4.769938e-56 | -8.615850e-47 |
| Msa0002700 | Msa0983720 | 0.832582 | 8.708425e-56 | -8.615850e-47 |
| Msa0016830 | Msa0983720 | 0.818337 | 2.066610e-52 | -8.615850e-47 |
| Msa0021570 | Msa0983720 | 0.832544 | 8.898353e-56 | -8.615850e-47 |
| Msa0028410 | Msa0983720 | 0.821215 | 4.546473e-53 | -8.615850e-47 |
| Msa0028450 | Msa0983720 | 0.829867 | 4.048351e-55 | -8.615850e-47 |
| Msa0028940 | Msa0983720 | 0.812250 | 4.659531e-51 | -8.615850e-47 |
| Msa0030710 | Msa0983720 | 0.823801 | 1.138854e-53 | -8.615850e-47 |
| Msa0035090 | Msa0983720 | 0.801088 | 1.061874e-48 | -8.615850e-47 |
| Msa0058970 | Msa0983720 | 0.813006 | 3.183423e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983720 | MtrunA17_Chr4g0015651 | 98.821 | 509 | 6 | 0 | 1 | 509 | 1 | 509 | 0.0 | 1004 |
| Msa0983720 | MtrunA17_Chr4g0016741 | 98.821 | 509 | 6 | 0 | 1 | 509 | 1 | 509 | 0.0 | 1004 |
| Msa0983720 | MtrunA17_CPg0492941 | 98.821 | 509 | 6 | 0 | 1 | 509 | 1 | 509 | 0.0 | 1004 |
| Msa0983720 | MtrunA17_MTg0490471 | 61.310 | 504 | 175 | 5 | 3 | 492 | 4 | 501 | 0.0 | 601 |
| Msa0983720 | MtrunA17_Chr2g0278821 | 91.538 | 260 | 22 | 0 | 221 | 480 | 2 | 261 | 4.68e-173 | 489 |
| Msa0983720 | MtrunA17_Chr2g0286451 | 91.803 | 183 | 15 | 0 | 79 | 261 | 7 | 189 | 4.91e-115 | 338 |
| Msa0983720 | MtrunA17_Chr4g0040631 | 96.748 | 123 | 4 | 0 | 1 | 123 | 1 | 123 | 1.09e-74 | 232 |
| Msa0983720 | MtrunA17_Chr4g0061471 | 89.333 | 75 | 8 | 0 | 100 | 174 | 1 | 75 | 1.92e-39 | 137 |
| Msa0983720 | MtrunA17_Chr1g0211291 | 30.363 | 303 | 191 | 5 | 87 | 371 | 84 | 384 | 5.22e-29 | 119 |
| Msa0983720 | MtrunA17_Chr7g0250051 | 30.132 | 302 | 192 | 5 | 87 | 371 | 83 | 382 | 1.82e-28 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983720 | ATCG00120.1 | 93.294 | 507 | 34 | 0 | 1 | 507 | 1 | 507 | 0.0 | 956 |
| Msa0983720 | AT2G07698.1 | 57.916 | 499 | 196 | 3 | 5 | 492 | 276 | 771 | 0.0 | 577 |
| Msa0983720 | ATMG01190.1 | 57.715 | 499 | 197 | 3 | 5 | 492 | 6 | 501 | 0.0 | 572 |
| Msa0983720 | AT4G38510.1 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0983720 | AT4G38510.3 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0983720 | AT4G38510.4 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0983720 | AT4G38510.2 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0983720 | AT4G38510.5 | 30.464 | 302 | 191 | 5 | 87 | 371 | 90 | 389 | 8.84e-30 | 122 |
| Msa0983720 | AT1G76030.1 | 30.464 | 302 | 191 | 5 | 87 | 371 | 82 | 381 | 7.41e-29 | 119 |
| Msa0983720 | AT1G20260.1 | 29.703 | 303 | 193 | 5 | 87 | 371 | 82 | 382 | 4.64e-28 | 117 |
Find 119 sgRNAs with CRISPR-Local
Find 260 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCATGAAACAGGTAGC+TGG | 0.558354 | 6_4:-26802287 | Msa0983720:CDS |
| AAATATTTGGCCATCTGTAA+TGG | 0.434228 | 6_4:+26802397 | None:intergenic |
| AAATGTTGGTGTTGTATTAA+TGG | 0.258988 | 6_4:-26803197 | Msa0983720:CDS |
| AAATTTCAGCTTCGGAATCT+CGG | 0.492871 | 6_4:-26803045 | Msa0983720:CDS |
| AACTACTTTACAAGAAAGAG+GGG | 0.635218 | 6_4:-26802777 | Msa0983720:CDS |
| AAGCTGAAACATCTCCTGAT+TGG | 0.409535 | 6_4:+26802441 | None:intergenic |
| AATCAGACCAGCTATTAATG+TGG | 0.625394 | 6_4:-26802348 | Msa0983720:CDS |
| AATGGAGTACACTATTATAG+TGG | 0.544433 | 6_4:-26802753 | Msa0983720:CDS |
| AATGTAATTTCCATTACAGA+TGG | 0.557336 | 6_4:-26802407 | Msa0983720:CDS |
| AATGTTGGTGTTGTATTAAT+GGG | 0.391664 | 6_4:-26803196 | Msa0983720:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATATTGCTCAATACGTTCA+CGG | + | chr6_4:26801937-26801956 | None:intergenic | 30.0% | |
| TGAATTAGTCGAATTTGAAG+AGG | - | chr6_4:26802048-26802067 | Msa0983720:CDS | 30.0% | |
| GAATTAGTCGAATTTGAAGA+GGG | - | chr6_4:26802049-26802068 | Msa0983720:CDS | 30.0% | |
| TTGATTCTATGATCCCTATA+GGG | - | chr6_4:26802356-26802375 | Msa0983720:CDS | 30.0% | |
| AGATACAATTCTCAATCAAC+AGG | - | chr6_4:26802438-26802457 | Msa0983720:CDS | 30.0% | |
| GATACAATTCTCAATCAACA+GGG | - | chr6_4:26802439-26802458 | Msa0983720:CDS | 30.0% | |
| GTAACTACTTTACAAGAAAG+AGG | - | chr6_4:26802523-26802542 | Msa0983720:CDS | 30.0% | |
| AACTACTTTACAAGAAAGAG+GGG | - | chr6_4:26802525-26802544 | Msa0983720:CDS | 30.0% | |
| AATGGAGTACACTATTATAG+TGG | - | chr6_4:26802549-26802568 | Msa0983720:CDS | 30.0% | |
| TTCGTAATAGAAGAGACATT+TGG | + | chr6_4:26802714-26802733 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 26801896 | 26803428 | 26801896 | ID=Msa0983720;Name=Msa0983720 |
| chr6_4 | mRNA | 26801896 | 26803428 | 26801896 | ID=Msa0983720-mRNA-1;Parent=Msa0983720;Name=Msa0983720-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|510 |
| chr6_4 | exon | 26801896 | 26803428 | 26801896 | ID=Msa0983720-mRNA-1:exon:7585;Parent=Msa0983720-mRNA-1 |
| chr6_4 | CDS | 26801896 | 26803428 | 26801896 | ID=Msa0983720-mRNA-1:cds;Parent=Msa0983720-mRNA-1 |
| Gene Sequence |
>Msa0983720 ATGGTAACCATTCGTGCAGATGAAATTAGTAAAATTATCCGTGAACGTATTGAGCAATATAATACCGAAGTAAAAATTGTAAATACAGGTACTGTACTTCAAGTAGGCGACGGCATTGCTCGTATTTATGGTCTTGATGAAGTAATGGCAGGTGAATTAGTCGAATTTGAAGAGGGTACTGTAGGCATTGCTTTGAATTTGGAATCCAAAAATGTTGGTGTTGTATTAATGGGTGATGGTTTGCTGATACAAGAGGGAAGTTCGGTAAAAGCAACAGGAAGAATTGCTCAGATACCAGTAAGTGAGGGTTATTTGGGTCGTGTTGTAAATGCCCTAGCTAAACCTATTGATGGTCGAGGAGAAATTTCAGCTTCGGAATCTCGGTTAATCGAATCTCCCGCTCCCGGTATTATTTCCAGACGTTCCGTGTATGAGCCTCTTCAAACGGGACTTATTGCTATTGATTCTATGATCCCTATAGGGCGTGGCCAGCGAGAATTAATTATTGGGGACAGACAAACAGGTAAAACAGCAGTAGCAACAGATACAATTCTCAATCAACAGGGACAAAATGTAATATGTGTTTATGTAGCTATTGGTCAAAAAGCATCTTCTGTGGCTCAAGTGGTAACTACTTTACAAGAAAGAGGGGCAATGGAGTACACTATTATAGTGGCTGAAACAGCAAATTCTCCAGCTACATTACAATACCTCGCTCCTTATACAGGTGCAGCTCTGGCTGAATATTTTATGTACCGTGAACGTCACACTTTAATCATTTATGACGATCCCTCCAAACAAGCACAGGCTTATCGCCAAATGTCTCTTCTATTACGAAGACCACCAGGTCGCGAAGCGTATCCCGGAGATGTTTTTTATTTACATTCTCGTCTTTTGGAAAGAGCCGCTAAATTAAGTTCTCAGTTAGGTGAAGGAAGTATGACTGCTTTACCAATAGTTGAGACCCAATCAGGAGATGTTTCAGCTTATATTCCTACTAATGTAATTTCCATTACAGATGGCCAAATATTTTTATCTGCCGATCTATTCAATGCTGGAATCAGACCAGCTATTAATGTGGGTATTTCCGTTTCCAGAGTTGGATCGGCGGCTCAAATTAAAGCCATGAAACAGGTAGCTGGTAAATTAAAATTGGAATTGGCACAATTCGCAGAATTAGAAGCTTTTGCACAATTCGCTTCTGATCTCGATAAAGCTACGCAAAATCAATTGGCAAGAGGTCAACGATTGCGCGAGTTGCTTAAACAATCCCAATCAGCTCCTCTCACTGTGGAAGAACAGATAATAACTATTTATACTGGAACGAATGGTTATCTTGATTCATTAGAAATTGATCAGGTACGTAAATTTCTCGTTGAGTTAAGGGCTTATTTAAAAACGAATAAACCTAAATTTAACGAAATCATATCTTCTACCAAGACATTCACTGGGGAAGCAGAAGCCCTTTTGAAGGAAGCTATTCAGGAACAGATGGAACTCTTTTTACTTCAGGAACAAGTAGAAAAAAATTGA |
| Protein sequence |
>Msa0983720 MVTIRADEISKIIRERIEQYNTEVKIVNTGTVLQVGDGIARIYGLDEVMAGELVEFEEGTVGIALNLESKNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEGYLGRVVNALAKPIDGRGEISASESRLIESPAPGIISRRSVYEPLQTGLIAIDSMIPIGRGQRELIIGDRQTGKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIIVAETANSPATLQYLAPYTGAALAEYFMYRERHTLIIYDDPSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSSQLGEGSMTALPIVETQSGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVGSAAQIKAMKQVAGKLKLELAQFAELEAFAQFASDLDKATQNQLARGQRLRELLKQSQSAPLTVEEQIITIYTGTNGYLDSLEIDQVRKFLVELRAYLKTNKPKFNEIISSTKTFTGEAEALLKEAIQEQMELFLLQEQVEKN* |
