AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368280 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368280 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368280 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368280 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368280 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368280 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1368280 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368280 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018631:-66235 Msa1368280:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018631:+66255 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018631:-66266 Msa1368280:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018631:-66566 Msa1368280:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018631:-66510 Msa1368280:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018631:-66207 Msa1368280:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018631:+66295 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018631:-66709 Msa1368280:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018631:+66386 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018631:+66260 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018631:+66441 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018631:-66234 Msa1368280:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018631:-66230 Msa1368280:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018631:+66686 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018631:+66281 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018631:-66545 Msa1368280:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018631:+66442 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018631:+66707 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018631:+66294 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018631:-66366 Msa1368280:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018631:+66368 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018631:+66526 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018631:-66698 Msa1368280:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018631:-66365 Msa1368280:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018631:+66449 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018631:+66276 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018631:+66298 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018631:+66296 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018631:-66766 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018631:-66767 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018631:-66554 Msa1368280:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018631:+66426 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018631:+66297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0018631:66704-66723 Msa1368280:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0018631:66705-66724 Msa1368280:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0018631:66732-66751 Msa1368280:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0018631:66331-66350 Msa1368280:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0018631:66416-66435 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0018631:66451-66470 Msa1368280:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0018631:66682-66701 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0018631:66687-66706 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0018631:66709-66728 Msa1368280:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0018631:66201-66220 Msa1368280:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0018631:66202-66221 Msa1368280:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0018631:66429-66448 Msa1368280:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0018631:66450-66469 Msa1368280:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0018631:66516-66535 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0018631:66514-66533 Msa1368280:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0018631:66574-66593 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0018631:66573-66592 Msa1368280:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0018631:66574-66593 Msa1368280:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0018631:66673-66692 Msa1368280:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0018631:66199-66218 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0018631:66203-66222 Msa1368280:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0018631:66230-66249 Msa1368280:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0018631:66456-66475 Msa1368280:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0018631:66500-66519 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0018631:66501-66520 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0018631:66204-66223 Msa1368280:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0018631:66282-66301 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0018631:66373-66392 Msa1368280:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0018631:66493-66512 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0018631:66556-66575 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0018631:66648-66667 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0018631:66661-66680 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0018631:66666-66685 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0018631:66241-66260 Msa1368280:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0018631:66256-66275 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0018631:66394-66413 Msa1368280:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0018631:66647-66666 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0018631:66235-66254 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0018631:66385-66404 Msa1368280:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0018631:66646-66665 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0018631:66644-66663 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0018631:66645-66664 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0018631:66214-66233 Msa1368280:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018631 gene 66178 66783 66178 ID=Msa1368280;Name=Msa1368280
tig0018631 mRNA 66178 66783 66178 ID=Msa1368280-mRNA-1;Parent=Msa1368280;Name=Msa1368280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018631 exon 66178 66783 66178 ID=Msa1368280-mRNA-1:exon:384;Parent=Msa1368280-mRNA-1
tig0018631 CDS 66178 66783 66178 ID=Msa1368280-mRNA-1:cds;Parent=Msa1368280-mRNA-1
Gene Sequence

>Msa1368280

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1368280

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*