AlfalfaGEDB | Msa1164450

M. sativa cultivar zhongmu-4 / Msa1164450

KEGG: -
GO: -
PFAM: PF14510.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1164450	XP_003625399.2	26.538	260	153	6	160	414	881	1107	6.57e-15	90.9
Msa1164450	XP_003625399.2	94.209	449	26	0	1	449	1	449	0.0	880

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1164450	sp\|Q76CU2\|PDR1_TOBAC	76.854	445	102	1	5	449	20	463	0.0	718
Msa1164450	sp\|Q76CU2\|PDR1_TOBAC	27.308	260	151	6	159	413	861	1087	6.91e-18	90.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1164450	G7KYF6	26.538	260	153	6	160	414	881	1107	3.14e-15	90.9
Msa1164450	G7KYF6	94.209	449	26	0	1	449	1	449	0.0	880

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0013270	Msa1164450	0.828309	9.657602e-55	-8.615850e-47
Msa0025570	Msa1164450	0.809428	1.901307e-50	-8.615850e-47
Msa0044310	Msa1164450	0.804956	1.683680e-49	-8.615850e-47
Msa0062010	Msa1164450	0.812799	3.534018e-51	-8.615850e-47
Msa0073110	Msa1164450	0.808378	3.189836e-50	-8.615850e-47
Msa0148670	Msa1164450	0.807538	4.814676e-50	-8.615850e-47
Msa0160380	Msa1164450	0.802207	6.260839e-49	-8.615850e-47
Msa0260840	Msa1164450	0.803394	3.560517e-49	-8.615850e-47
Msa0261400	Msa1164450	0.813484	2.500850e-51	-8.615850e-47
Msa0279810	Msa1164450	0.822904	1.845221e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1164450	MtrunA17_Chr7g0262231	94.209	449	26	0	1	449	1	449	0.0	880
Msa1164450	MtrunA17_Chr7g0262231	26.538	260	153	6	160	414	881	1107	6.04e-19	90.9
Msa1164450	MtrunA17_Chr7g0261981	93.987	449	27	0	1	449	1	449	0.0	877
Msa1164450	MtrunA17_Chr7g0261981	25.979	281	168	7	160	433	859	1106	6.71e-19	90.5
Msa1164450	MtrunA17_Chr7g0261951	93.987	449	27	0	1	449	1	449	0.0	872
Msa1164450	MtrunA17_Chr7g0261951	26.335	281	167	7	160	433	877	1124	2.04e-18	89.0
Msa1164450	MtrunA17_Chr7g0261971	93.541	449	25	1	1	449	1	445	0.0	863
Msa1164450	MtrunA17_Chr7g0262241	93.318	449	30	0	1	449	1	449	0.0	850
Msa1164450	MtrunA17_Chr7g0262241	27.526	287	167	8	160	438	878	1131	8.29e-21	96.7
Msa1164450	MtrunA17_Chr7g0262221	88.419	449	34	1	1	449	2	432	0.0	816

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1164450	AT1G15520.1	68.707	441	136	1	11	449	16	456	0.0	661
Msa1164450	AT1G15520.1	27.170	265	155	6	155	414	845	1076	9.29e-19	90.5
Msa1164450	AT1G15520.2	68.707	441	136	1	11	449	16	456	0.0	660
Msa1164450	AT1G15520.2	27.170	265	155	6	155	414	845	1076	7.16e-19	90.9
Msa1164450	AT2G36380.1	62.181	431	159	2	22	448	44	474	0.0	569
Msa1164450	AT1G66950.1	61.298	447	166	3	9	448	30	476	0.0	569
Msa1164450	AT1G66950.1	26.437	261	154	6	159	414	877	1104	1.83e-20	95.9
Msa1164450	AT1G15210.1	58.963	463	176	3	1	449	9	471	0.0	557
Msa1164450	AT1G15210.1	28.322	286	165	7	156	434	861	1113	8.25e-23	103
Msa1164450	AT1G59870.1	56.962	474	182	3	32	497	48	507	0.0	554

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Find 99 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTGATCCAAAATTGA+AGG	0.512290	7_4:+56125657	Msa1164450:CDS
AAAAGACCAAGAGCAATACT+GGG	0.629897	7_4:+56127323	Msa1164450:CDS
AAACTTTGGCCTTCTCAGCA+AGG	0.565835	7_4:+56125928	Msa1164450:CDS
AAAGTTTCTGCTCAAACTTA+AGG	0.388000	7_4:+56125043	Msa1164450:CDS
AACCACACTCCTGTTGGCTT+TGG	0.354490	7_4:+56125630	Msa1164450:CDS
AACTTAAGGATCGAATGGAC+AGG	0.561378	7_4:+56125057	Msa1164450:CDS
AACTTTGGCCTTCTCAGCAA+GGG	0.549423	7_4:+56125929	Msa1164450:CDS
AAGTTTCTGGCGGCGGCTGA+AGG	0.494464	7_4:-56127065	None:intergenic
AATAGATTGTAAGTTTCTGG+CGG	0.663428	7_4:-56127075	None:intergenic
AATATTCTCAAGGATGTTAG+TGG	0.453666	7_4:+56125464	Msa1164450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGATGAAGAAGCTCTTAAA+TGG	+	chr7_4:56124862-56124881	Msa1164450:CDS	30.0%
TGATGAAGAAGCTCTTAAAT+GGG	+	chr7_4:56124863-56124882	Msa1164450:CDS	30.0%
AAAGTTTCTGCTCAAACTTA+AGG	+	chr7_4:56125043-56125062	Msa1164450:CDS	30.0%
AATATTCTCAAGGATGTTAG+TGG	+	chr7_4:56125464-56125483	Msa1164450:CDS	30.0%
GATCAAAATGATCTTCACAT+TGG	+	chr7_4:56125888-56125907	Msa1164450:CDS	30.0%
CTATTTCAGTAGCAAAAGAT+AGG	+	chr7_4:56126177-56126196	Msa1164450:intron	30.0%
AATGTCAACAATATTACACG+TGG	-	chr7_4:56126393-56126412	None:intergenic	30.0%
TGATAACAGATTATGTCCTA+AGG	+	chr7_4:56126634-56126653	Msa1164450:CDS	30.0%
GATAACAGATTATGTCCTAA+GGG	+	chr7_4:56126635-56126654	Msa1164450:CDS	30.0%
TTCATGGATGAAATATCTAC+GGG	+	chr7_4:56126958-56126977	Msa1164450:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	56124768	56128121	56124768	ID=Msa1164450;Name=Msa1164450
chr7_4	mRNA	56124768	56128121	56124768	ID=Msa1164450-mRNA-1;Parent=Msa1164450;Name=Msa1164450-mRNA-1;_AED=0.95;_eAED=0.95;_QI=0\|0\|0\|0.16\|1\|1\|12\|0\|497
chr7_4	exon	56124768	56125078	56124768	ID=Msa1164450-mRNA-1:exon:16699;Parent=Msa1164450-mRNA-1
chr7_4	exon	56125187	56125307	56125187	ID=Msa1164450-mRNA-1:exon:16700;Parent=Msa1164450-mRNA-1
chr7_4	exon	56125416	56125504	56125416	ID=Msa1164450-mRNA-1:exon:16701;Parent=Msa1164450-mRNA-1
chr7_4	exon	56125594	56125678	56125594	ID=Msa1164450-mRNA-1:exon:16702;Parent=Msa1164450-mRNA-1
chr7_4	exon	56125816	56125975	56125816	ID=Msa1164450-mRNA-1:exon:16703;Parent=Msa1164450-mRNA-1
chr7_4	exon	56126419	56126495	56126419	ID=Msa1164450-mRNA-1:exon:16704;Parent=Msa1164450-mRNA-1
chr7_4	exon	56126603	56126656	56126603	ID=Msa1164450-mRNA-1:exon:16705;Parent=Msa1164450-mRNA-1
chr7_4	exon	56126753	56126843	56126753	ID=Msa1164450-mRNA-1:exon:16706;Parent=Msa1164450-mRNA-1
chr7_4	exon	56126926	56127227	56126926	ID=Msa1164450-mRNA-1:exon:16707;Parent=Msa1164450-mRNA-1
chr7_4	exon	56127312	56127380	56127312	ID=Msa1164450-mRNA-1:exon:16708;Parent=Msa1164450-mRNA-1
chr7_4	exon	56127857	56127956	56127857	ID=Msa1164450-mRNA-1:exon:16709;Parent=Msa1164450-mRNA-1
chr7_4	exon	56128087	56128121	56128087	ID=Msa1164450-mRNA-1:exon:16710;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56124768	56125078	56124768	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56125187	56125307	56125187	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56125416	56125504	56125416	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56125594	56125678	56125594	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56125816	56125975	56125816	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56126419	56126495	56126419	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56126603	56126656	56126603	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56126753	56126843	56126753	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56126926	56127227	56126926	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56127312	56127380	56127312	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56127857	56127956	56127857	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1
chr7_4	CDS	56128087	56128121	56128087	ID=Msa1164450-mRNA-1:cds;Parent=Msa1164450-mRNA-1

Gene Sequence

>Msa1164450

ATGGAGGGTGGAGGTAGTTTCAGGATTGGTAGTTCATCAATTTGGAGGAACAGTGATGCTGCTGAGATTTTTTCAAATTCTTTCCACCAAGAAGATGATGAAGAAGCTCTTAAATGGGCTGCAATTCAGAAACTTCCTACTTTTGAACGTTTGAGGAAAGGTTTGCTCACTTCACTTCAAGGGGAAGCCATTGAGATCGATATTGAGAAGCTTGGACTGCAAGAAAGAAAAGATTTGCTTGAAAGACTTGTTAGACTTGCTGAAGAGGATAATGAAAAGTTTCTGCTCAAACTTAAGGATCGAATGGACAGAGTTGGAATTGATCTTCCTACAATAGAAGTTCGATTCGAGCACCTGAATATCGAAGCAGAAGCTCGTGTAGGAAGCAGATCTTTGCCTACCTTCACTAATTTCATGGTTAATATAGTAGAGGGCCTATTGAACTCTCTTCATCTACTTCCAAGTAGAAAACAACATCTAAATATTCTCAAGGATGTTAGTGGAATATTAAAGCCTTCCAGAATGACATTGCTTTTAGGTCCTCCAAGTTCTGGAAAAACCACACTCCTGTTGGCTTTGGCCGGAAAACTTGATCCAAAATTGAAGTTTTCTGGTAGGGTGACTTATAATGGTCATGAGATGAGTGAATTTGTCCCCCAAAGAACTGCTGCTTATGTGGATCAAAATGATCTTCACATTGGAGAGTTGACTGTTAGAGAAACTTTGGCCTTCTCAGCAAGGGTCCAAGGAGTTGGACCTCGTTATGAGTTGCTAGCAGAATTGTCCAGAAGAGAAAAACATGCAAATATCAAGCCTGATCCAGATCTTGATGTGTACATGAAAGCTGTAGCAAATGAAGGCCAGAAGGAAAATTTGATAACAGATTATGTCCTAAGGATTTTGGGACTAGAGATATGTGCTGATACTGTTGTGGGAAATGCAATGATAAGAGGTATCTCTGGCGGACAAAAGAAACGCCTTACAACAGGGGAGATGTTGGTTGGACCAACTAAAGCTTTATTCATGGATGAAATATCTACGGGTTTGGATAGCTCGACAACTTTTCAAATTGTGAATTCAATGAAGCAATATGTCCACATTCTCAAAGGAACCGCGGTGATCTCACTCCTTCAGCCGCCGCCAGAAACTTACAATCTATTTGACAACATTATTCTACTCTCTGATAGTCACACTATATACCAAGGTCCTCGGGAACACGTGCTTGAATTTTTCGAATCAATTGGTTTTAAATGTCCAAATAGGAAAGGAGTGGCAGATTTTCTGCAAGAAGTGACATCAGTAAAAGACCAAGAGCAATACTGGGAACACAAAGATCAGTCTTATAGATGCGATGATAAAATATACGAGTTATTATACGTCATGATGGTTTTTGATGAAGGAGCAGAAGTAGCTGGACCATATAAAGTACACGTCAGTGGTACTATTTTTGAGTTAACAGAGCAGGTCGATTCAGATCGTGCCTTGGGTCATTAG

Protein sequence

>Msa1164450

MEGGGSFRIGSSSIWRNSDAAEIFSNSFHQEDDEEALKWAAIQKLPTFERLRKGLLTSLQGEAIEIDIEKLGLQERKDLLERLVRLAEEDNEKFLLKLKDRMDRVGIDLPTIEVRFEHLNIEAEARVGSRSLPTFTNFMVNIVEGLLNSLHLLPSRKQHLNILKDVSGILKPSRMTLLLGPPSSGKTTLLLALAGKLDPKLKFSGRVTYNGHEMSEFVPQRTAAYVDQNDLHIGELTVRETLAFSARVQGVGPRYELLAELSRREKHANIKPDPDLDVYMKAVANEGQKENLITDYVLRILGLEICADTVVGNAMIRGISGGQKKRLTTGEMLVGPTKALFMDEISTGLDSSTTFQIVNSMKQYVHILKGTAVISLLQPPPETYNLFDNIILLSDSHTIYQGPREHVLEFFESIGFKCPNRKGVADFLQEVTSVKDQEQYWEHKDQSYRCDDKIYELLYVMMVFDEGAEVAGPYKVHVSGTIFELTEQVDSDRALGH*