AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1225230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225230 MtrunA17_Chr3g0130981 97.071 239 7 0 1 239 385 623 2.94e-167 475
Msa1225230 MtrunA17_Chr8g0385111 70.661 242 68 2 1 239 399 640 4.07e-102 309
Msa1225230 MtrunA17_Chr1g0200831 54.918 244 102 5 1 238 408 649 2.36e-77 245
Msa1225230 MtrunA17_Chr3g0142751 36.111 252 129 5 1 236 369 604 8.36e-41 147
Msa1225230 MtrunA17_Chr1g0156671 40.314 191 89 3 1 177 374 553 1.98e-36 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225230 AT1G34320.1 51.600 250 110 5 1 239 406 655 2.91e-75 240
Msa1225230 AT1G34320.2 51.600 250 110 5 1 239 406 655 2.91e-75 240
Msa1225230 AT5G08660.3 50.410 244 111 5 1 239 398 636 1.44e-70 228
Msa1225230 AT5G08660.1 50.410 244 111 5 1 239 398 636 1.44e-70 228
Msa1225230 AT5G08660.4 50.410 244 111 5 1 239 398 636 1.44e-70 228
Msa1225230 AT1G30755.5 49.007 151 61 2 1 147 381 519 4.58e-40 145
Msa1225230 AT1G30755.1 49.007 151 61 2 1 147 381 519 4.58e-40 145
Msa1225230 AT1G30755.6 49.007 151 61 2 1 147 381 519 4.58e-40 145
Msa1225230 AT5G08660.2 74.324 74 19 0 1 74 398 471 5.64e-35 130
Msa1225230 AT1G30755.3 61.644 73 28 0 1 73 381 453 3.77e-26 105
Msa1225230 AT1G30755.4 55.932 59 26 0 1 59 381 439 9.03e-15 73.2
Msa1225230 AT1G30755.2 55.932 59 26 0 1 59 381 439 9.03e-15 73.2

Find 100 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAATGTTTCCAATTAT+TGG 0.118201 8_1:-90567300 None:intergenic
ATTGTCCACATTATTGTATT+AGG 0.168999 8_1:+90569518 Msa1225230:three_prime_UTR
GAACGTAAGAGTGGGATTTC+TGG 0.216885 8_1:+90569367 Msa1225230:three_prime_UTR
TTCAGAGCACATCATGGTTT+TGG 0.272685 8_1:+90568648 Msa1225230:intron
CTGCTTATTTGTCTCTTCTA+AGG 0.275008 8_1:-90569004 None:intergenic
TTCATTAAGGTGCATTGTTT+TGG 0.275031 8_1:+90569470 Msa1225230:three_prime_UTR
ATATTCTTGAACTTCTCTTA+TGG 0.275314 8_1:+90568899 Msa1225230:CDS
GACCAGCAGGGCCCAATCTT+TGG 0.285927 8_1:-90567326 None:intergenic
TGTTTCCAATTATTGGCTTT+TGG 0.291648 8_1:-90567293 None:intergenic
TTGTCCACATTATTGTATTA+GGG 0.291715 8_1:+90569519 Msa1225230:three_prime_UTR
GATGGCCAAAAGCCAATAAT+TGG 0.293410 8_1:+90567288 Msa1225230:intron
AACATGAAAAGATGGCAATT+TGG 0.298938 8_1:-90567823 None:intergenic
GGATTCCATCGGCTTCATTA+AGG 0.320977 8_1:+90569457 Msa1225230:three_prime_UTR
AATGTATCCCTTGTATTTGC+AGG 0.329699 8_1:-90567759 None:intergenic
ATCACTCTGGTGTAGAAGTT+TGG 0.333610 8_1:+90566632 Msa1225230:five_prime_UTR
AATGTTATGTCATTGATTTA+TGG 0.334294 8_1:-90569714 None:intergenic
ATACTGCAAAGTCAGACTTA+TGG 0.344695 8_1:-90566461 None:intergenic
TACTGCAAAGTCAGACTTAT+GGG 0.350252 8_1:-90566460 None:intergenic
AATGTGACCCCACTCGTGTT+GGG 0.358258 8_1:+90569578 Msa1225230:three_prime_UTR
CTGCCATCAAGCCCTCTGTT+GGG 0.364695 8_1:+90568977 Msa1225230:CDS
GTTGGGGCATGGTGGAATTA+CGG 0.370864 8_1:+90569595 Msa1225230:three_prime_UTR
AACGTAAGAGTGGGATTTCT+GGG 0.384692 8_1:+90569368 Msa1225230:three_prime_UTR
TGGAGATAAGCAATAACTTT+GGG 0.384872 8_1:+90566926 Msa1225230:five_prime_UTR
TTGGATGTGTTTGTGGCAAT+TGG 0.385614 8_1:-90568504 None:intergenic
CGCCAAAGATTGGGCCCTGC+TGG 0.389746 8_1:+90567324 Msa1225230:five_prime_UTR
CTGGTTGGCTTTGAAATGAA+TGG 0.394663 8_1:+90569324 Msa1225230:three_prime_UTR
CATTGGCAATCGCCAAAGAT+TGG 0.403466 8_1:+90567314 Msa1225230:five_prime_UTR
TTGGAGATAAGCAATAACTT+TGG 0.406389 8_1:+90566925 Msa1225230:five_prime_UTR
TCTCTTATGGCTTCACCGCT+TGG 0.426438 8_1:+90568912 Msa1225230:CDS
AGTATCACTCTTCCCCTCAT+AGG 0.434328 8_1:+90566540 Msa1225230:five_prime_UTR
TTTAATGCTTTCAGGTAGCC+AGG 0.440062 8_1:+90567724 Msa1225230:intron
TAATGCAAAGCAAGACCAGC+AGG 0.444099 8_1:-90567339 None:intergenic
GGAGAAAACATTACATTGGT+TGG 0.450537 8_1:+90568479 Msa1225230:CDS
AGCAAGGCTGGCAGTAATGC+TGG 0.457453 8_1:+90568943 Msa1225230:CDS
CTTTGTCTTTATCAGCATAG+TGG 0.465065 8_1:-90568867 None:intergenic
AGCAATAACTTTGGGAGTGC+AGG 0.470375 8_1:+90566934 Msa1225230:five_prime_UTR
ATTGGCAATCGCCAAAGATT+GGG 0.470486 8_1:+90567315 Msa1225230:five_prime_UTR
TCTGCCATCAAGCCCTCTGT+TGG 0.475672 8_1:+90568976 Msa1225230:CDS
ATCTTACTTGGATGTGTTTG+TGG 0.476717 8_1:-90568511 None:intergenic
TTGGCCATCAAAAGCAAGGC+TGG 0.477527 8_1:+90568931 Msa1225230:CDS
GAAAGGAATTAGTAAAAGCT+TGG 0.479183 8_1:+90569104 Msa1225230:CDS
TAATTGCAAGTTTGACTGGT+TGG 0.485904 8_1:+90569309 Msa1225230:exon
CAGATAATTGCAAGTTTGAC+TGG 0.487641 8_1:+90569305 Msa1225230:exon
ACCCCACTCGTGTTGGGGCA+TGG 0.488402 8_1:+90569584 Msa1225230:three_prime_UTR
AGCCCCAACAGAGGGCTTGA+TGG 0.488501 8_1:-90568980 None:intergenic
CAATGTGACCCCACTCGTGT+TGG 0.492349 8_1:+90569577 Msa1225230:three_prime_UTR
AATTGTTCTTGAAGCTCTGA+GGG 0.493262 8_1:+90568799 Msa1225230:intron
AAGCCAATAATTGGAAACAT+TGG 0.494783 8_1:+90567297 Msa1225230:five_prime_UTR
TTTCTATGCCTGCAAATACA+AGG 0.495725 8_1:+90567751 Msa1225230:exon
TCTTCTACAACATGAAAAGA+TGG 0.498353 8_1:-90567831 None:intergenic
GTAGGGGAGTGGGCGAACAC+AGG 0.498543 8_1:+90568675 Msa1225230:CDS
AGTAAGCATGAACGTAAGAG+TGG 0.502391 8_1:+90569358 Msa1225230:three_prime_UTR
ATGCAAGGCTATCACCTATG+AGG 0.511296 8_1:-90566554 None:intergenic
TGCCATCAAGCCCTCTGTTG+GGG 0.522975 8_1:+90568978 Msa1225230:CDS
GAGCAGAAATTAAAAGTCAA+AGG 0.524821 8_1:+90566583 Msa1225230:five_prime_UTR
CGATGAGCAAAACATGCTGC+AGG 0.529082 8_1:+90569059 Msa1225230:CDS
CTGCACTTCTCACTCAATGT+AGG 0.533270 8_1:-90569144 None:intergenic
TGCAAGGCTATCACCTATGA+GGG 0.536787 8_1:-90566553 None:intergenic
AAATACTAAATGAGTTAATG+AGG 0.542458 8_1:+90569639 Msa1225230:three_prime_UTR
CACCATGCCCCAACACGAGT+GGG 0.547474 8_1:-90569586 None:intergenic
TGATGAAAGACTGCTACTCT+TGG 0.549167 8_1:-90569172 None:intergenic
AATGAAGCCGATGGAATCCA+TGG 0.556462 8_1:-90569453 None:intergenic
TAGGGGAGTGGGCGAACACA+GGG 0.558056 8_1:+90568676 Msa1225230:CDS
ATGTGACCCCACTCGTGTTG+GGG 0.558808 8_1:+90569579 Msa1225230:three_prime_UTR
AGCGGATATCAAAGATGAGA+TGG 0.560104 8_1:+90568458 Msa1225230:CDS
TCAATATCAAAATCAATCAC+AGG 0.562537 8_1:-90569240 None:intergenic
TTGCAGCAACTACGGTGCCA+TGG 0.566967 8_1:+90569436 Msa1225230:three_prime_UTR
ACTCTGGTGTAGAAGTTTGG+AGG 0.567281 8_1:+90566635 Msa1225230:five_prime_UTR
TTGTATTAGGGAAATTGTTG+AGG 0.568322 8_1:+90569531 Msa1225230:three_prime_UTR
TTTCTGCTCTCAAGAATGCA+AGG 0.568733 8_1:-90566569 None:intergenic
AGAATGCAATTCTGAAAGGA+TGG 0.569881 8_1:+90566392 None:intergenic
AATCGGTTCAGAGCACATCA+TGG 0.579088 8_1:+90568642 Msa1225230:intron
TCTTTCATCAAGTAAAAGCA+AGG 0.583845 8_1:+90569185 Msa1225230:CDS
TAATTGTTCTTGAAGCTCTG+AGG 0.593353 8_1:+90568798 Msa1225230:intron
CAATCCGCATTGCACCACCT+TGG 0.598917 8_1:-90568837 None:intergenic
TACGGTGCCATGGATTCCAT+CGG 0.602752 8_1:+90569446 Msa1225230:three_prime_UTR
AGATGGAGAAAACATTACAT+TGG 0.603209 8_1:+90568475 Msa1225230:CDS
ATGCACCTTAATGAAGCCGA+TGG 0.604707 8_1:-90569462 None:intergenic
CTTTCATCAAGTAAAAGCAA+GGG 0.605567 8_1:+90569186 Msa1225230:CDS
CCGCTTGGCCATCAAAAGCA+AGG 0.607199 8_1:+90568927 Msa1225230:CDS
GAGGGGAAGAGTGATACTGC+AGG 0.607230 8_1:-90566535 None:intergenic
ATTTCCCTAATACAATAATG+TGG 0.609557 8_1:-90569523 None:intergenic
TTCTATGCCTGCAAATACAA+GGG 0.614906 8_1:+90567752 Msa1225230:exon
CCACCATGCCCCAACACGAG+TGG 0.615269 8_1:-90569587 None:intergenic
CTGGTGTAGAAGTTTGGAGG+AGG 0.624347 8_1:+90566638 Msa1225230:five_prime_UTR
GTAAGCATGAACGTAAGAGT+GGG 0.630327 8_1:+90569359 Msa1225230:three_prime_UTR
ACGTAAGAGTGGGATTTCTG+GGG 0.631813 8_1:+90569369 Msa1225230:three_prime_UTR
CGTAAGAGTGGGATTTCTGG+GGG 0.632275 8_1:+90569370 Msa1225230:three_prime_UTR
TCTAAGGTAGCCCCAACAGA+GGG 0.637864 8_1:-90568988 None:intergenic
ACCATGCCCCAACACGAGTG+GGG 0.646354 8_1:-90569585 None:intergenic
CGTGATCGACAGACTAGATG+TGG 0.646686 8_1:+90569281 Msa1225230:CDS
TGCAGGCATAGAAATAGACC+TGG 0.654826 8_1:-90567742 None:intergenic
CCACTCGTGTTGGGGCATGG+TGG 0.659223 8_1:+90569587 Msa1225230:three_prime_UTR
GGTTACACAGCTCACTGTAG+CGG 0.667160 8_1:+90568440 Msa1225230:intron
AATGCAAAGCAAGACCAGCA+GGG 0.681812 8_1:-90567338 None:intergenic
ATTGTTCTTGAAGCTCTGAG+GGG 0.692125 8_1:+90568800 Msa1225230:intron
TTCTAAGGTAGCCCCAACAG+AGG 0.695918 8_1:-90568989 None:intergenic
ACAATGACATATCTACATTG+CGG 0.696432 8_1:-90569500 None:intergenic
GCAAGGCTATCACCTATGAG+GGG 0.722319 8_1:-90566552 None:intergenic
GTAAGAGTGGGATTTCTGGG+GGG 0.722382 8_1:+90569371 Msa1225230:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATCAATTACAAAATA+AGG - chr8_1:90567116-90567135 None:intergenic 10.0%
!!! TGATAAATTTTAAGTTTATA+AGG + chr8_1:90567127-90567146 Msa1225230:intron 10.0%
!! TAATAGAGAATTAAACAAAT+GGG - chr8_1:90566710-90566729 None:intergenic 15.0%
!! TTAATAGAGAATTAAACAAA+TGG - chr8_1:90566711-90566730 None:intergenic 15.0%
!!! TTAATTTTTATTCATACAGA+TGG + chr8_1:90566788-90566807 Msa1225230:intron 15.0%
!!! TAATTTTTATTCATACAGAT+GGG + chr8_1:90566789-90566808 Msa1225230:intron 15.0%
!!! TATTGTACATTTTTTACATT+TGG + chr8_1:90566906-90566925 Msa1225230:five_prime_UTR 15.0%
!!! TAAAGCTAATTTTATATTAG+GGG - chr8_1:90567799-90567818 None:intergenic 15.0%
!!! GTAAAGCTAATTTTATATTA+GGG - chr8_1:90567800-90567819 None:intergenic 15.0%
!! AAAAATCAGACTAAAAATTA+TGG + chr8_1:90567899-90567918 Msa1225230:intron 15.0%
!!! AAAATCTTAAATTTGTTTCT+TGG + chr8_1:90568385-90568404 Msa1225230:intron 15.0%
!! ATTTAAAGAAAAAATCACTC+TGG + chr8_1:90566619-90566638 Msa1225230:five_prime_UTR 20.0%
!! CTAATAGTTATTACATTAAG+CGG - chr8_1:90567020-90567039 None:intergenic 20.0%
!!! AATAAAAAGTGTCTACTTTT+TGG + chr8_1:90567150-90567169 Msa1225230:intron 20.0%
!!! ATAAAAAGTGTCTACTTTTT+GGG + chr8_1:90567151-90567170 Msa1225230:intron 20.0%
!!! AAAGCTAATTTTATATTAGG+GGG - chr8_1:90567798-90567817 None:intergenic 20.0%
!!! CGTAAAGCTAATTTTATATT+AGG - chr8_1:90567801-90567820 None:intergenic 20.0%
!!! AATTTAGAATAGATACAGAA+AGG + chr8_1:90567930-90567949 Msa1225230:intron 20.0%
!! TATTGAGTATTCATTATGAT+GGG - chr8_1:90568064-90568083 None:intergenic 20.0%
!! TTATTGAGTATTCATTATGA+TGG - chr8_1:90568065-90568084 None:intergenic 20.0%
!! TCATATAAGAGAATAAGTAT+TGG - chr8_1:90568234-90568253 None:intergenic 20.0%
!!! TGTTTTTGTTGTTTCAAAAT+CGG + chr8_1:90568625-90568644 Msa1225230:intron 20.0%
!! TAATTCAAAATAGAACAGTA+GGG - chr8_1:90568714-90568733 None:intergenic 20.0%
!! AGTAAGAAAACAAAAATGAA+AGG + chr8_1:90569087-90569106 Msa1225230:CDS 20.0%
!! AAATACTAAATGAGTTAATG+AGG + chr8_1:90569639-90569658 Msa1225230:three_prime_UTR 20.0%
!!! AATGTTATGTCATTGATTTA+TGG - chr8_1:90569717-90569736 None:intergenic 20.0%
!! AAAAAAAAAAGTAGCGATAT+AGG - chr8_1:90569740-90569759 None:intergenic 20.0%
!!! TATCGCTACTTTTTTTTTTT+TGG + chr8_1:90569741-90569760 Msa1225230:three_prime_UTR 20.0%
!!! ATCGCTACTTTTTTTTTTTT+GGG + chr8_1:90569742-90569761 Msa1225230:three_prime_UTR 20.0%
! GTAATACTGAAGATAGTATA+GGG + chr8_1:90566763-90566782 Msa1225230:intron 25.0%
!! TGAGTATACTCAAATTTTCT+CGG - chr8_1:90566848-90566867 None:intergenic 25.0%
! TGAGTATACTCATAATCTAA+TGG + chr8_1:90566856-90566875 Msa1225230:intron 25.0%
!! TGTTAATGTTGTTTATCCAT+GGG + chr8_1:90567257-90567276 Msa1225230:intron 25.0%
!! TTTAAGTATCTAGTTTCTTC+TGG + chr8_1:90567502-90567521 Msa1225230:intron 25.0%
! AATGCTAATCAATACAAAAC+AGG - chr8_1:90567671-90567690 None:intergenic 25.0%
! GGATTAAAAAATCAATAGCA+TGG - chr8_1:90567703-90567722 None:intergenic 25.0%
!! TTAATCCTTTTAATGCTTTC+AGG + chr8_1:90567716-90567735 Msa1225230:intron 25.0%
!! TAAGAATAGAAAGCATAAGT+TGG - chr8_1:90567868-90567887 None:intergenic 25.0%
!! CGATTTCTAAGAAAAATCAA+GGG - chr8_1:90568091-90568110 None:intergenic 25.0%
!! ACGATTTCTAAGAAAAATCA+AGG - chr8_1:90568092-90568111 None:intergenic 25.0%
!! TTGATTTTTCTTAGAAATCG+TGG + chr8_1:90568091-90568110 Msa1225230:intron 25.0%
! GGATAAATGTTTATAAAAGG+GGG - chr8_1:90568164-90568183 None:intergenic 25.0%
! CGGATAAATGTTTATAAAAG+GGG - chr8_1:90568165-90568184 None:intergenic 25.0%
! CCGGATAAATGTTTATAAAA+GGG - chr8_1:90568166-90568185 None:intergenic 25.0%
!! CCCTTTTATAAACATTTATC+CGG + chr8_1:90568163-90568182 Msa1225230:intron 25.0%
!! CCTTTTATAAACATTTATCC+GGG + chr8_1:90568164-90568183 Msa1225230:intron 25.0%
!!! AATTTAAGATTTTCTGCAGT+GGG - chr8_1:90568378-90568397 None:intergenic 25.0%
!!! AAATTTAAGATTTTCTGCAG+TGG - chr8_1:90568379-90568398 None:intergenic 25.0%
! CTAATTCAAAATAGAACAGT+AGG - chr8_1:90568715-90568734 None:intergenic 25.0%
! GAAGTACAGAAAAAACAAAA+GGG - chr8_1:90568754-90568773 None:intergenic 25.0%
!! AGAAGTACAGAAAAAACAAA+AGG - chr8_1:90568755-90568774 None:intergenic 25.0%
! ATATTCTTGAACTTCTCTTA+TGG + chr8_1:90568899-90568918 Msa1225230:CDS 25.0%
! TCGATTGTTAAAAATGATGA+CGG - chr8_1:90569042-90569061 None:intergenic 25.0%
! TCAATATCAAAATCAATCAC+AGG - chr8_1:90569243-90569262 None:intergenic 25.0%
! ATTGTCCACATTATTGTATT+AGG + chr8_1:90569518-90569537 Msa1225230:three_prime_UTR 25.0%
! TTGTCCACATTATTGTATTA+GGG + chr8_1:90569519-90569538 Msa1225230:three_prime_UTR 25.0%
! ATTTCCCTAATACAATAATG+TGG - chr8_1:90569526-90569545 None:intergenic 25.0%
GAGCAGAAATTAAAAGTCAA+AGG + chr8_1:90566583-90566602 Msa1225230:five_prime_UTR 30.0%
TAGAGAATTAAACAAATGGG+AGG - chr8_1:90566707-90566726 None:intergenic 30.0%
CAGTATTACCAATTTACATG+TGG - chr8_1:90566752-90566771 None:intergenic 30.0%
GGTAATACTGAAGATAGTAT+AGG + chr8_1:90566762-90566781 Msa1225230:intron 30.0%
TTGGAGATAAGCAATAACTT+TGG + chr8_1:90566925-90566944 Msa1225230:five_prime_UTR 30.0%
TGGAGATAAGCAATAACTTT+GGG + chr8_1:90566926-90566945 Msa1225230:five_prime_UTR 30.0%
! CTGTTAATGTTGTTTATCCA+TGG + chr8_1:90567256-90567275 Msa1225230:intron 30.0%
!!! TGTTTCCAATTATTGGCTTT+TGG - chr8_1:90567296-90567315 None:intergenic 30.0%
AAGCCAATAATTGGAAACAT+TGG + chr8_1:90567297-90567316 Msa1225230:five_prime_UTR 30.0%
TTGCCAATGTTTCCAATTAT+TGG - chr8_1:90567303-90567322 None:intergenic 30.0%
! ATCATATTTTCACCTGATTC+AGG - chr8_1:90567451-90567470 None:intergenic 30.0%
ACATTCAGATTTCATGTTGT+TGG + chr8_1:90567552-90567571 Msa1225230:intron 30.0%
ACTTTCAGATTTCATGTTGT+TGG + chr8_1:90567586-90567605 Msa1225230:intron 30.0%
CTTTCAGATTTCATGTTGTT+GGG + chr8_1:90567587-90567606 Msa1225230:intron 30.0%
ATCAATACAAAACAGGTTTG+TGG - chr8_1:90567664-90567683 None:intergenic 30.0%
AAAAATCAATAGCATGGACT+CGG - chr8_1:90567697-90567716 None:intergenic 30.0%
AACATGAAAAGATGGCAATT+TGG - chr8_1:90567826-90567845 None:intergenic 30.0%
TCTTCTACAACATGAAAAGA+TGG - chr8_1:90567834-90567853 None:intergenic 30.0%
! ATCTTTTCATGTTGTAGAAG+AGG + chr8_1:90567833-90567852 Msa1225230:CDS 30.0%
!! AGTCTGATTTTTCAACAAAG+CGG - chr8_1:90567891-90567910 None:intergenic 30.0%
CAGACTAAAAATTATGGATC+TGG + chr8_1:90567905-90567924 Msa1225230:intron 30.0%
AATAGATACAGAAAGGCTTT+AGG + chr8_1:90567937-90567956 Msa1225230:intron 30.0%
! TTTTTCTTAGAAATCGTGGT+TGG + chr8_1:90568095-90568114 Msa1225230:intron 30.0%
! TTTTCTTAGAAATCGTGGTT+GGG + chr8_1:90568096-90568115 Msa1225230:intron 30.0%
CCCGGATAAATGTTTATAAA+AGG - chr8_1:90568167-90568186 None:intergenic 30.0%
!!! GTTTTTTTGTTCTTTCTTCG+TGG + chr8_1:90568407-90568426 Msa1225230:intron 30.0%
AGATGGAGAAAACATTACAT+TGG + chr8_1:90568475-90568494 Msa1225230:CDS 30.0%
AAAAAGCCAGATATCTTACT+TGG - chr8_1:90568526-90568545 None:intergenic 30.0%
! AATAAGAAAGTTTTCCAAGG+TGG + chr8_1:90568823-90568842 Msa1225230:CDS 30.0%
! GATTGTTAAAAATGATGACG+GGG - chr8_1:90569040-90569059 None:intergenic 30.0%
CGATTGTTAAAAATGATGAC+GGG - chr8_1:90569041-90569060 None:intergenic 30.0%
GAAAGGAATTAGTAAAAGCT+TGG + chr8_1:90569104-90569123 Msa1225230:CDS 30.0%
TCTTTCATCAAGTAAAAGCA+AGG + chr8_1:90569185-90569204 Msa1225230:CDS 30.0%
CTTTCATCAAGTAAAAGCAA+GGG + chr8_1:90569186-90569205 Msa1225230:CDS 30.0%
!! CAATATTTTTGCAGCAACTA+CGG + chr8_1:90569428-90569447 Msa1225230:three_prime_UTR 30.0%
! TTCATTAAGGTGCATTGTTT+TGG + chr8_1:90569470-90569489 Msa1225230:three_prime_UTR 30.0%
ACAATGACATATCTACATTG+CGG - chr8_1:90569503-90569522 None:intergenic 30.0%
TTGTATTAGGGAAATTGTTG+AGG + chr8_1:90569531-90569550 Msa1225230:three_prime_UTR 30.0%
TACTGCAAAGTCAGACTTAT+GGG - chr8_1:90566463-90566482 None:intergenic 35.0%
ATACTGCAAAGTCAGACTTA+TGG - chr8_1:90566464-90566483 None:intergenic 35.0%
AGAATTAAACAAATGGGAGG+AGG - chr8_1:90566704-90566723 None:intergenic 35.0%
AGTCTGATCCACATGTAAAT+TGG + chr8_1:90566741-90566760 Msa1225230:intron 35.0%
!! CTCATAATCTAATGGATTGC+AGG + chr8_1:90566864-90566883 Msa1225230:intron 35.0%
TTATTACATTAAGCGGCACT+AGG - chr8_1:90567013-90567032 None:intergenic 35.0%
TGAGGAAAAAAGAACAGTCA+TGG - chr8_1:90567427-90567446 None:intergenic 35.0%
!!! TTTTTTCCTCATCCTGAATC+AGG + chr8_1:90567436-90567455 Msa1225230:intron 35.0%
TGAAATCTGAATGTAGCAGA+AGG - chr8_1:90567546-90567565 None:intergenic 35.0%
TTTCTATGCCTGCAAATACA+AGG + chr8_1:90567751-90567770 Msa1225230:exon 35.0%
TTCTATGCCTGCAAATACAA+GGG + chr8_1:90567752-90567771 Msa1225230:exon 35.0%
AATGTATCCCTTGTATTTGC+AGG - chr8_1:90567762-90567781 None:intergenic 35.0%
! TGAGTATTCATTATGATGGG+TGG - chr8_1:90568061-90568080 None:intergenic 35.0%
AACAGACTACTAAAGTACGA+AGG - chr8_1:90568261-90568280 None:intergenic 35.0%
GGAGAAAACATTACATTGGT+TGG + chr8_1:90568479-90568498 Msa1225230:CDS 35.0%
ATCTTACTTGGATGTGTTTG+TGG - chr8_1:90568514-90568533 None:intergenic 35.0%
ACACATCCAAGTAAGATATC+TGG + chr8_1:90568517-90568536 Msa1225230:intron 35.0%
!!! GATTAAAGTTGCTTTTCACG+TGG - chr8_1:90568569-90568588 None:intergenic 35.0%
TAATTGTTCTTGAAGCTCTG+AGG + chr8_1:90568798-90568817 Msa1225230:intron 35.0%
! AATTGTTCTTGAAGCTCTGA+GGG + chr8_1:90568799-90568818 Msa1225230:intron 35.0%
! GGGAATAAGAAAGTTTTCCA+AGG + chr8_1:90568820-90568839 Msa1225230:CDS 35.0%
CTTTGTCTTTATCAGCATAG+TGG - chr8_1:90568870-90568889 None:intergenic 35.0%
CTGCTTATTTGTCTCTTCTA+AGG - chr8_1:90569007-90569026 None:intergenic 35.0%
!! AAAAATGATGACGGGGAATT+TGG - chr8_1:90569033-90569052 None:intergenic 35.0%
! GCAAGGGATTGTCTTTTAAT+AGG + chr8_1:90569202-90569221 Msa1225230:CDS 35.0%
CAGATAATTGCAAGTTTGAC+TGG + chr8_1:90569305-90569324 Msa1225230:exon 35.0%
TAATTGCAAGTTTGACTGGT+TGG + chr8_1:90569309-90569328 Msa1225230:exon 35.0%
CGTAGTTGCTGCAAAAATAT+TGG - chr8_1:90569430-90569449 None:intergenic 35.0%
TTAAAGCTGTGCAAAAACGA+AGG - chr8_1:90569674-90569693 None:intergenic 35.0%
TTTCTGCTCTCAAGAATGCA+AGG - chr8_1:90566572-90566591 None:intergenic 40.0%
ATCACTCTGGTGTAGAAGTT+TGG + chr8_1:90566632-90566651 Msa1225230:five_prime_UTR 40.0%
AAACAAATGGGAGGAGGATA+TGG - chr8_1:90566698-90566717 None:intergenic 40.0%
GATGGCCAAAAGCCAATAAT+TGG + chr8_1:90567288-90567307 Msa1225230:intron 40.0%
ATTGGCAATCGCCAAAGATT+GGG + chr8_1:90567315-90567334 Msa1225230:five_prime_UTR 40.0%
! TTTTCACCTGATTCAGGATG+AGG - chr8_1:90567445-90567464 None:intergenic 40.0%
!! GGCTACCTGAAAGCATTAAA+AGG - chr8_1:90567724-90567743 None:intergenic 40.0%
! TTTAATGCTTTCAGGTAGCC+AGG + chr8_1:90567724-90567743 Msa1225230:intron 40.0%
AACTGAAGAGGGACTTTAGT+TGG + chr8_1:90567992-90568011 Msa1225230:intron 40.0%
GGCTACTAATGTTAACTAGG+TGG - chr8_1:90568040-90568059 None:intergenic 40.0%
GGTGGCTACTAATGTTAACT+AGG - chr8_1:90568043-90568062 None:intergenic 40.0%
TTAACACAACCCCACAAAAC+CGG + chr8_1:90568120-90568139 Msa1225230:intron 40.0%
AGTGGGATGTGTAAAGCTAA+GGG - chr8_1:90568361-90568380 None:intergenic 40.0%
AGCGGATATCAAAGATGAGA+TGG + chr8_1:90568458-90568477 Msa1225230:CDS 40.0%
TTGGATGTGTTTGTGGCAAT+TGG - chr8_1:90568507-90568526 None:intergenic 40.0%
TTTGTCAACAAGCTCCTTAG+TGG + chr8_1:90568543-90568562 Msa1225230:intron 40.0%
! TTCAGAGCACATCATGGTTT+TGG + chr8_1:90568648-90568667 Msa1225230:intron 40.0%
!! ATTGTTCTTGAAGCTCTGAG+GGG + chr8_1:90568800-90568819 Msa1225230:intron 40.0%
TGATGAAAGACTGCTACTCT+TGG - chr8_1:90569175-90569194 None:intergenic 40.0%
!! CTGGTTGGCTTTGAAATGAA+TGG + chr8_1:90569324-90569343 Msa1225230:three_prime_UTR 40.0%
AGTAAGCATGAACGTAAGAG+TGG + chr8_1:90569358-90569377 Msa1225230:three_prime_UTR 40.0%
GTAAGCATGAACGTAAGAGT+GGG + chr8_1:90569359-90569378 Msa1225230:three_prime_UTR 40.0%
AACGTAAGAGTGGGATTTCT+GGG + chr8_1:90569368-90569387 Msa1225230:three_prime_UTR 40.0%
! GTGGAATTACGGTTTTCAGT+CGG + chr8_1:90569606-90569625 Msa1225230:three_prime_UTR 40.0%
AGTATCACTCTTCCCCTCAT+AGG + chr8_1:90566540-90566559 Msa1225230:five_prime_UTR 45.0%
TGCAAGGCTATCACCTATGA+GGG - chr8_1:90566556-90566575 None:intergenic 45.0%
ATGCAAGGCTATCACCTATG+AGG - chr8_1:90566557-90566576 None:intergenic 45.0%
ACTCTGGTGTAGAAGTTTGG+AGG + chr8_1:90566635-90566654 Msa1225230:five_prime_UTR 45.0%
! AGCAATAACTTTGGGAGTGC+AGG + chr8_1:90566934-90566953 Msa1225230:five_prime_UTR 45.0%
GCAGGTCTGGATCTACTTAA+AGG + chr8_1:90566952-90566971 Msa1225230:intron 45.0%
TATCCATGGGTACTTGCAGA+TGG + chr8_1:90567270-90567289 Msa1225230:intron 45.0%
CATTGGCAATCGCCAAAGAT+TGG + chr8_1:90567314-90567333 Msa1225230:five_prime_UTR 45.0%
AATGCAAAGCAAGACCAGCA+GGG - chr8_1:90567341-90567360 None:intergenic 45.0%
TAATGCAAAGCAAGACCAGC+AGG - chr8_1:90567342-90567361 None:intergenic 45.0%
TGCAGGCATAGAAATAGACC+TGG - chr8_1:90567745-90567764 None:intergenic 45.0%
!! TCAGTTGTGTTGTGGACTAG+AGG - chr8_1:90567978-90567997 None:intergenic 45.0%
AGTCCACAACACAACTGAAG+AGG + chr8_1:90567980-90567999 Msa1225230:intron 45.0%
GTCCACAACACAACTGAAGA+GGG + chr8_1:90567981-90568000 Msa1225230:intron 45.0%
CAGTGGGATGTGTAAAGCTA+AGG - chr8_1:90568362-90568381 None:intergenic 45.0%
TTTCTTCGTGGTTGCTCTCT+GGG + chr8_1:90568419-90568438 Msa1225230:intron 45.0%
! AATCGGTTCAGAGCACATCA+TGG + chr8_1:90568642-90568661 Msa1225230:intron 45.0%
!!! AGCACATCATGGTTTTGGCT+GGG + chr8_1:90568653-90568672 Msa1225230:CDS 45.0%
!!! ATCATGGTTTTGGCTGGGTA+GGG + chr8_1:90568658-90568677 Msa1225230:CDS 45.0%
! TTTTCCAAGGTGGTGCAATG+CGG + chr8_1:90568833-90568852 Msa1225230:CDS 45.0%
CTGCACTTCTCACTCAATGT+AGG - chr8_1:90569147-90569166 None:intergenic 45.0%
GAACGTAAGAGTGGGATTTC+TGG + chr8_1:90569367-90569386 Msa1225230:three_prime_UTR 45.0%
ACGTAAGAGTGGGATTTCTG+GGG + chr8_1:90569369-90569388 Msa1225230:three_prime_UTR 45.0%
AATGAAGCCGATGGAATCCA+TGG - chr8_1:90569456-90569475 None:intergenic 45.0%
!! GGATTCCATCGGCTTCATTA+AGG + chr8_1:90569457-90569476 Msa1225230:three_prime_UTR 45.0%
ATGCACCTTAATGAAGCCGA+TGG - chr8_1:90569465-90569484 None:intergenic 45.0%
!! TTAAGTATTTATAATAAAAA+AGG - chr8_1:90568781-90568800 None:intergenic 5.0%
GCAAGGCTATCACCTATGAG+GGG - chr8_1:90566555-90566574 None:intergenic 50.0%
CTGGTGTAGAAGTTTGGAGG+AGG + chr8_1:90566638-90566657 Msa1225230:five_prime_UTR 50.0%
! TAACTTTGGGAGTGCAGGTC+TGG + chr8_1:90566939-90566958 Msa1225230:intron 50.0%
GTCCCTCTTCAGTTGTGTTG+TGG - chr8_1:90567986-90568005 None:intergenic 50.0%
!! ATCGCACAAGCCGGTTTTGT+GGG - chr8_1:90568133-90568152 None:intergenic 50.0%
ACATCAGTTGAGAGATGGCC+CGG - chr8_1:90568185-90568204 None:intergenic 50.0%
GGCCATCTCTCAACTGATGT+GGG + chr8_1:90568185-90568204 Msa1225230:intron 50.0%
GTCCCACATCAGTTGAGAGA+TGG - chr8_1:90568190-90568209 None:intergenic 50.0%
CTTTCTTCGTGGTTGCTCTC+TGG + chr8_1:90568418-90568437 Msa1225230:intron 50.0%
GGTTACACAGCTCACTGTAG+CGG + chr8_1:90568440-90568459 Msa1225230:intron 50.0%
! TGCTTTTCACGTGGCCACTA+AGG - chr8_1:90568560-90568579 None:intergenic 50.0%
!!! GAGCACATCATGGTTTTGGC+TGG + chr8_1:90568652-90568671 Msa1225230:intron 50.0%
!!! CATCATGGTTTTGGCTGGGT+AGG + chr8_1:90568657-90568676 Msa1225230:CDS 50.0%
!!! TCATGGTTTTGGCTGGGTAG+GGG + chr8_1:90568659-90568678 Msa1225230:CDS 50.0%
TCTCTTATGGCTTCACCGCT+TGG + chr8_1:90568912-90568931 Msa1225230:CDS 50.0%
!! CCTTGCTTTTGATGGCCAAG+CGG - chr8_1:90568930-90568949 None:intergenic 50.0%
TTGGCCATCAAAAGCAAGGC+TGG + chr8_1:90568931-90568950 Msa1225230:CDS 50.0%
!! ACTGCCAGCCTTGCTTTTGA+TGG - chr8_1:90568938-90568957 None:intergenic 50.0%
! TCTAAGGTAGCCCCAACAGA+GGG - chr8_1:90568991-90569010 None:intergenic 50.0%
!! TTCTAAGGTAGCCCCAACAG+AGG - chr8_1:90568992-90569011 None:intergenic 50.0%
CGATGAGCAAAACATGCTGC+AGG + chr8_1:90569059-90569078 Msa1225230:CDS 50.0%
CGTGATCGACAGACTAGATG+TGG + chr8_1:90569281-90569300 Msa1225230:CDS 50.0%
! CGTAAGAGTGGGATTTCTGG+GGG + chr8_1:90569370-90569389 Msa1225230:three_prime_UTR 50.0%
! GTAAGAGTGGGATTTCTGGG+GGG + chr8_1:90569371-90569390 Msa1225230:three_prime_UTR 50.0%
!! TACGGTGCCATGGATTCCAT+CGG + chr8_1:90569446-90569465 Msa1225230:three_prime_UTR 50.0%
AATGTGACCCCACTCGTGTT+GGG + chr8_1:90569578-90569597 Msa1225230:three_prime_UTR 50.0%
! GTTGGGGCATGGTGGAATTA+CGG + chr8_1:90569595-90569614 Msa1225230:three_prime_UTR 50.0%
GAGGGGAAGAGTGATACTGC+AGG - chr8_1:90566538-90566557 None:intergenic 55.0%
TGGCCATCTGCAAGTACCCA+TGG - chr8_1:90567276-90567295 None:intergenic 55.0%
!! TCGCACAAGCCGGTTTTGTG+GGG - chr8_1:90568132-90568151 None:intergenic 55.0%
!! CATCGCACAAGCCGGTTTTG+TGG - chr8_1:90568134-90568153 None:intergenic 55.0%
GCAATCAGCATCGCACAAGC+CGG - chr8_1:90568142-90568161 None:intergenic 55.0%
GGGCCATCTCTCAACTGATG+TGG + chr8_1:90568184-90568203 Msa1225230:intron 55.0%
!! TTTTGGCTGGGTAGGGGAGT+GGG + chr8_1:90568665-90568684 Msa1225230:CDS 55.0%
CAATCCGCATTGCACCACCT+TGG - chr8_1:90568840-90568859 None:intergenic 55.0%
CCGCTTGGCCATCAAAAGCA+AGG + chr8_1:90568927-90568946 Msa1225230:CDS 55.0%
AGCAAGGCTGGCAGTAATGC+TGG + chr8_1:90568943-90568962 Msa1225230:CDS 55.0%
TCTGCCATCAAGCCCTCTGT+TGG + chr8_1:90568976-90568995 Msa1225230:CDS 55.0%
CTGCCATCAAGCCCTCTGTT+GGG + chr8_1:90568977-90568996 Msa1225230:CDS 55.0%
TGCCATCAAGCCCTCTGTTG+GGG + chr8_1:90568978-90568997 Msa1225230:CDS 55.0%
! TTGCAGCAACTACGGTGCCA+TGG + chr8_1:90569436-90569455 Msa1225230:three_prime_UTR 55.0%
CAATGTGACCCCACTCGTGT+TGG + chr8_1:90569577-90569596 Msa1225230:three_prime_UTR 55.0%
ATGTGACCCCACTCGTGTTG+GGG + chr8_1:90569579-90569598 Msa1225230:three_prime_UTR 55.0%
GACCAGCAGGGCCCAATCTT+TGG - chr8_1:90567329-90567348 None:intergenic 60.0%
!!! GTTTTGGCTGGGTAGGGGAG+TGG + chr8_1:90568664-90568683 Msa1225230:CDS 60.0%
TAGGGGAGTGGGCGAACACA+GGG + chr8_1:90568676-90568695 Msa1225230:CDS 60.0%
AGCCCCAACAGAGGGCTTGA+TGG - chr8_1:90568983-90569002 None:intergenic 60.0%
ACCATGCCCCAACACGAGTG+GGG - chr8_1:90569588-90569607 None:intergenic 60.0%
CACCATGCCCCAACACGAGT+GGG - chr8_1:90569589-90569608 None:intergenic 60.0%
CGCCAAAGATTGGGCCCTGC+TGG + chr8_1:90567324-90567343 Msa1225230:five_prime_UTR 65.0%
GTAGGGGAGTGGGCGAACAC+AGG + chr8_1:90568675-90568694 Msa1225230:CDS 65.0%
ACCCCACTCGTGTTGGGGCA+TGG + chr8_1:90569584-90569603 Msa1225230:three_prime_UTR 65.0%
CCACCATGCCCCAACACGAG+TGG - chr8_1:90569590-90569609 None:intergenic 65.0%
CCACTCGTGTTGGGGCATGG+TGG + chr8_1:90569587-90569606 Msa1225230:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 90566404 90569776 90566404 ID=Msa1225230;Name=Msa1225230
chr8_1 mRNA 90566404 90569776 90566404 ID=Msa1225230-mRNA-1;Parent=Msa1225230;Name=Msa1225230-mRNA-1;_AED=0.06;_eAED=0.06;_QI=448|1|1|1|1|1|7|465|239
chr8_1 exon 90566404 90566659 90566404 ID=Msa1225230-mRNA-1:exon:32902;Parent=Msa1225230-mRNA-1
chr8_1 exon 90566886 90566955 90566886 ID=Msa1225230-mRNA-1:exon:32903;Parent=Msa1225230-mRNA-1
chr8_1 exon 90567289 90567392 90567289 ID=Msa1225230-mRNA-1:exon:32904;Parent=Msa1225230-mRNA-1
chr8_1 exon 90567738 90567854 90567738 ID=Msa1225230-mRNA-1:exon:32905;Parent=Msa1225230-mRNA-1
chr8_1 exon 90568450 90568526 90568450 ID=Msa1225230-mRNA-1:exon:32906;Parent=Msa1225230-mRNA-1
chr8_1 exon 90568653 90568697 90568653 ID=Msa1225230-mRNA-1:exon:32907;Parent=Msa1225230-mRNA-1
chr8_1 exon 90568813 90569776 90568813 ID=Msa1225230-mRNA-1:exon:32908;Parent=Msa1225230-mRNA-1
chr8_1 five_prime_UTR 90566404 90566659 90566404 ID=Msa1225230-mRNA-1:five_prime_utr;Parent=Msa1225230-mRNA-1
chr8_1 five_prime_UTR 90566886 90566955 90566886 ID=Msa1225230-mRNA-1:five_prime_utr;Parent=Msa1225230-mRNA-1
chr8_1 five_prime_UTR 90567289 90567392 90567289 ID=Msa1225230-mRNA-1:five_prime_utr;Parent=Msa1225230-mRNA-1
chr8_1 five_prime_UTR 90567738 90567755 90567738 ID=Msa1225230-mRNA-1:five_prime_utr;Parent=Msa1225230-mRNA-1
chr8_1 CDS 90567756 90567854 90567756 ID=Msa1225230-mRNA-1:cds;Parent=Msa1225230-mRNA-1
chr8_1 CDS 90568450 90568526 90568450 ID=Msa1225230-mRNA-1:cds;Parent=Msa1225230-mRNA-1
chr8_1 CDS 90568653 90568697 90568653 ID=Msa1225230-mRNA-1:cds;Parent=Msa1225230-mRNA-1
chr8_1 CDS 90568813 90569311 90568813 ID=Msa1225230-mRNA-1:cds;Parent=Msa1225230-mRNA-1
chr8_1 three_prime_UTR 90569312 90569776 90569312 ID=Msa1225230-mRNA-1:three_prime_utr;Parent=Msa1225230-mRNA-1
Gene Sequence

>Msa1225230

ATGCCTGCAAATACAAGGGATACATTGTATCAAAACTTGCCCCCTAATATAAAATTAGCTTTACGTTCCAAATTGCCATCTTTTCATGTTGTAGAAGAGCTCACTGTAGCGGATATCAAAGATGAGATGGAGAAAACATTACATTGGTTGGTTCCAATTGCCACAAACACATCCAAAGCACATCATGGTTTTGGCTGGGTAGGGGAGTGGGCGAACACAGGCTCTGAGGGGAATAAGAAAGTTTTCCAAGGTGGTGCAATGCGGATTGAGACATTCCACTATGCTGATAAAGACAAAGTAGAACATTATATTCTTGAACTTCTCTTATGGCTTCACCGCTTGGCCATCAAAAGCAAGGCTGGCAGTAATGCTGGCAAAGTGAAGTCTGCCATCAAGCCCTCTGTTGGGGCTACCTTAGAAGAGACAAATAAGCAGTCTCCAAATTCCCCGTCATCATTTTTAACAATCGATGAGCAAAACATGCTGCAGGATGTAAGTAAGAAAACAAAAATGAAAGGAATTAGTAAAAGCTTGGACTTTGACAGTCTGAAGCCTACATTGAGTGAGAAGTGCAGACTGACCAAGAGTAGCAGTCTTTCATCAAGTAAAAGCAAGGGATTGTCTTTTAATAGGATTTTGTCAAAGCTTCCTGTGATTGATTTTGATATTGACAAAGAACGAGCATTGAACGTGATCGACAGACTAGATGTGGTCAGATAA
Protein sequence

>Msa1225230

MPANTRDTLYQNLPPNIKLALRSKLPSFHVVEELTVADIKDEMEKTLHWLVPIATNTSKAHHGFGWVGEWANTGSEGNKKVFQGGAMRIETFHYADKDKVEHYILELLLWLHRLAIKSKAGSNAGKVKSAIKPSVGATLEETNKQSPNSPSSFLTIDEQNMLQDVSKKTKMKGISKSLDFDSLKPTLSEKCRLTKSSSLSSSKSKGLSFNRILSKLPVIDFDIDKERALNVIDRLDVVR*