AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1359030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359030 MtrunA17_Chr6g0450151 79.787 94 19 0 23 116 329 422 1.06e-43 151
Msa1359030 MtrunA17_Chr6g0450071 72.340 94 26 0 23 116 269 362 9.39e-40 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCAAAATTATCCTTAAA+AGG 0.203644 tig0016634:+3768 Msa1359030:intergenic
TTCTGTTGGCGAATTTGTTT+GGG 0.261695 tig0016634:-3938 Msa1359030:CDS
TTTCTGTTGGCGAATTTGTT+TGG 0.262300 tig0016634:-3939 Msa1359030:CDS
ATGCTCTCTGTGCCACTATC+TGG 0.338217 tig0016634:+3567 None:intergenic
AATATTCGTTCTGTTTCTGC+TGG 0.350235 tig0016634:-2920 None:intergenic
AGTCGTTCGTTTGTTGTTGC+GGG 0.355271 tig0016634:-3657 Msa1359030:CDS
AAATGAGGTTGGAAGGGTTC+TGG 0.372760 tig0016634:-3691 Msa1359030:CDS
AACAGCATAGGTAAAAGCTT+TGG 0.424351 tig0016634:+3725 Msa1359030:intergenic
AAGAGTCATCCGTGGTGGCC+AGG 0.469996 tig0016634:-3907 Msa1359030:intron
TAGTGGCACAGAGAGCATTT+CGG 0.477803 tig0016634:-3562 Msa1359030:CDS
CAAGAAGCTGTAAATGAGGT+TGG 0.503519 tig0016634:-3702 Msa1359030:CDS
GAGTCGTTCGTTTGTTGTTG+CGG 0.521739 tig0016634:-3658 Msa1359030:CDS
GTCTGAATTCGCTCCAATGT+TGG 0.525621 tig0016634:-3625 Msa1359030:CDS
ATATTCGTTCTGTTTCTGCT+GGG 0.537547 tig0016634:-2919 None:intergenic
AAGCTGTAAATGAGGTTGGA+AGG 0.558828 tig0016634:-3698 Msa1359030:CDS
TTGACGATTTGATCGATGAA+AGG 0.570904 tig0016634:-3969 None:intergenic
GTCGTTCGTTTGTTGTTGCG+GGG 0.581074 tig0016634:-3656 Msa1359030:CDS
AGCTGTAAATGAGGTTGGAA+GGG 0.590294 tig0016634:-3697 Msa1359030:CDS
GAAAGGTGTGACATTTCTGT+TGG 0.595681 tig0016634:-3952 Msa1359030:CDS
TACAGCTTCTTGAACAGCAT+AGG 0.602664 tig0016634:+3713 Msa1359030:intergenic
TCGTTCGTTTGTTGTTGCGG+GGG 0.630767 tig0016634:-3655 Msa1359030:CDS
TAAACCCAACCTGGCCACCA+CGG 0.639794 tig0016634:+3898 Msa1359030:intergenic
TGTTCAAGAAGCTGTAAATG+AGG 0.656142 tig0016634:-3706 Msa1359030:CDS
TTTGTTGCAGATTTGCGATG+AGG 0.676368 tig0016634:-2960 Msa1359030:intron
TCTGTTGGCGAATTTGTTTG+GGG 0.678938 tig0016634:-3937 Msa1359030:CDS
TTTGATGATATGGTGAGACG+AGG 0.681159 tig0016634:-3753 Msa1359030:CDS
TGGCACAGAGAGCATTTCGG+CGG 0.682197 tig0016634:-3559 Msa1359030:intron
AGATTAAGAGTCATCCGTGG+TGG 0.700219 tig0016634:-3912 Msa1359030:CDS
GAAAGATTAAGAGTCATCCG+TGG 0.746341 tig0016634:-3915 Msa1359030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATCAAAATTATCCTTAAA+AGG + tig0016634:3111-3130 Msa1359030:intergenic 20.0%
!!! TAAGGATAATTTTGATGATA+TGG - tig0016634:3113-3132 Msa1359030:intron 20.0%
!! ATCAAAAGCCATAAATAATT+CGG + tig0016634:3429-3448 Msa1359030:intergenic 20.0%
!! AAAGAAATGAACAAACAATA+AGG - tig0016634:3678-3697 Msa1359030:CDS 20.0%
!!! TTTTTACAGTGTTTTACTTA+TGG + tig0016634:3745-3764 Msa1359030:intergenic 20.0%
!! TCATCAAAATTATCCTTAAA+AGG + tig0016634:3111-3130 Msa1359030:intergenic 20.0%
!!! TAAGGATAATTTTGATGATA+TGG - tig0016634:3113-3132 Msa1359030:intron 20.0%
!! ATCAAAAGCCATAAATAATT+CGG + tig0016634:3429-3448 Msa1359030:intergenic 20.0%
!! AAAGAAATGAACAAACAATA+AGG - tig0016634:3678-3697 Msa1359030:CDS 20.0%
!!! TTTTTACAGTGTTTTACTTA+TGG + tig0016634:3745-3764 Msa1359030:intergenic 20.0%
! CCAAATTCAAGAATTGTATT+TGG + tig0016634:3497-3516 Msa1359030:intergenic 25.0%
! CCAAATACAATTCTTGAATT+TGG - tig0016634:3494-3513 Msa1359030:intron 25.0%
! CTTGAATTTGGATAATTTCA+TGG - tig0016634:3506-3525 Msa1359030:intron 25.0%
! TGGATAATTTCATGGTTATT+TGG - tig0016634:3514-3533 Msa1359030:intron 25.0%
! TATTTCATTGCAATTCTGTT+TGG - tig0016634:3615-3634 Msa1359030:CDS 25.0%
! ATATATTTGAGCATGAGAAT+AGG - tig0016634:3878-3897 Msa1359030:intron 25.0%
! CCAAATTCAAGAATTGTATT+TGG + tig0016634:3497-3516 Msa1359030:intergenic 25.0%
! CCAAATACAATTCTTGAATT+TGG - tig0016634:3494-3513 Msa1359030:intron 25.0%
! CTTGAATTTGGATAATTTCA+TGG - tig0016634:3506-3525 Msa1359030:intron 25.0%
! TGGATAATTTCATGGTTATT+TGG - tig0016634:3514-3533 Msa1359030:intron 25.0%
! TATTTCATTGCAATTCTGTT+TGG - tig0016634:3615-3634 Msa1359030:CDS 25.0%
! ATATATTTGAGCATGAGAAT+AGG - tig0016634:3878-3897 Msa1359030:intron 25.0%
TTAAAAGGCTTCAATTGTGA+CGG + tig0016634:3096-3115 Msa1359030:intergenic 30.0%
AAAAATTCAGGAATCAAGGA+AGG - tig0016634:3276-3295 Msa1359030:intron 30.0%
CAGAATTGCAATGAAATACA+AGG + tig0016634:3613-3632 Msa1359030:intergenic 30.0%
GAAATCTCTATACATTCAGT+AGG - tig0016634:3835-3854 Msa1359030:intron 30.0%
TTAAAAGGCTTCAATTGTGA+CGG + tig0016634:3096-3115 Msa1359030:intergenic 30.0%
AAAAATTCAGGAATCAAGGA+AGG - tig0016634:3276-3295 Msa1359030:intron 30.0%
CAGAATTGCAATGAAATACA+AGG + tig0016634:3613-3632 Msa1359030:intergenic 30.0%
GAAATCTCTATACATTCAGT+AGG - tig0016634:3835-3854 Msa1359030:intron 30.0%
! TTTCTGTTGGCGAATTTGTT+TGG - tig0016634:2937-2956 Msa1359030:CDS 35.0%
! TTCTGTTGGCGAATTTGTTT+GGG - tig0016634:2938-2957 Msa1359030:CDS 35.0%
CTGAAACAAGAATAGACTCT+TGG - tig0016634:3035-3054 Msa1359030:intron 35.0%
ACTTTGATGGAACATTTGCT+TGG - tig0016634:3064-3083 Msa1359030:intron 35.0%
TAAAAGGCTTCAATTGTGAC+GGG + tig0016634:3095-3114 Msa1359030:intergenic 35.0%
! GTCACAATTGAAGCCTTTTA+AGG - tig0016634:3095-3114 Msa1359030:intron 35.0%
AACAGCATAGGTAAAAGCTT+TGG + tig0016634:3154-3173 Msa1359030:intergenic 35.0%
TGTTCAAGAAGCTGTAAATG+AGG - tig0016634:3170-3189 Msa1359030:intron 35.0%
TAGTTGCGCCGAATTATTTA+TGG - tig0016634:3418-3437 Msa1359030:intron 35.0%
ATTTGAGCATGAGAATAGGA+AGG - tig0016634:3882-3901 Msa1359030:intron 35.0%
! TTTCTGTTGGCGAATTTGTT+TGG - tig0016634:2937-2956 Msa1359030:CDS 35.0%
! TTCTGTTGGCGAATTTGTTT+GGG - tig0016634:2938-2957 Msa1359030:CDS 35.0%
CTGAAACAAGAATAGACTCT+TGG - tig0016634:3035-3054 Msa1359030:intron 35.0%
ACTTTGATGGAACATTTGCT+TGG - tig0016634:3064-3083 Msa1359030:intron 35.0%
TAAAAGGCTTCAATTGTGAC+GGG + tig0016634:3095-3114 Msa1359030:intergenic 35.0%
! GTCACAATTGAAGCCTTTTA+AGG - tig0016634:3095-3114 Msa1359030:intron 35.0%
AACAGCATAGGTAAAAGCTT+TGG + tig0016634:3154-3173 Msa1359030:intergenic 35.0%
TGTTCAAGAAGCTGTAAATG+AGG - tig0016634:3170-3189 Msa1359030:intron 35.0%
TAGTTGCGCCGAATTATTTA+TGG - tig0016634:3418-3437 Msa1359030:intron 35.0%
ATTTGAGCATGAGAATAGGA+AGG - tig0016634:3882-3901 Msa1359030:intron 35.0%
! GAAAGGTGTGACATTTCTGT+TGG - tig0016634:2924-2943 Msa1359030:CDS 40.0%
! TCTGTTGGCGAATTTGTTTG+GGG - tig0016634:2939-2958 Msa1359030:CDS 40.0%
GAAAGATTAAGAGTCATCCG+TGG - tig0016634:2961-2980 Msa1359030:intron 40.0%
! CTCTTGGTAGCTTACTTTGA+TGG - tig0016634:3051-3070 Msa1359030:intron 40.0%
! TTTGATGATATGGTGAGACG+AGG - tig0016634:3123-3142 Msa1359030:intron 40.0%
TACAGCTTCTTGAACAGCAT+AGG + tig0016634:3166-3185 Msa1359030:intergenic 40.0%
CAAGAAGCTGTAAATGAGGT+TGG - tig0016634:3174-3193 Msa1359030:intron 40.0%
AAGCTGTAAATGAGGTTGGA+AGG - tig0016634:3178-3197 Msa1359030:intron 40.0%
AGCTGTAAATGAGGTTGGAA+GGG - tig0016634:3179-3198 Msa1359030:intron 40.0%
! CCTGAATTTTTCGCCAACAT+TGG + tig0016634:3267-3286 Msa1359030:intergenic 40.0%
CCAATGTTGGCGAAAAATTC+AGG - tig0016634:3264-3283 Msa1359030:intron 40.0%
GGCGAAAAATTCAGGAATCA+AGG - tig0016634:3272-3291 Msa1359030:intron 40.0%
TACAACGTTGTAGCTGTTGT+AGG - tig0016634:3537-3556 Msa1359030:intron 40.0%
TTTGTTGCAGATTTGCGATG+AGG - tig0016634:3916-3935 Msa1359030:CDS 40.0%
! GAAAGGTGTGACATTTCTGT+TGG - tig0016634:2924-2943 Msa1359030:CDS 40.0%
! TCTGTTGGCGAATTTGTTTG+GGG - tig0016634:2939-2958 Msa1359030:CDS 40.0%
GAAAGATTAAGAGTCATCCG+TGG - tig0016634:2961-2980 Msa1359030:intron 40.0%
! CTCTTGGTAGCTTACTTTGA+TGG - tig0016634:3051-3070 Msa1359030:intron 40.0%
! TTTGATGATATGGTGAGACG+AGG - tig0016634:3123-3142 Msa1359030:intron 40.0%
TACAGCTTCTTGAACAGCAT+AGG + tig0016634:3166-3185 Msa1359030:intergenic 40.0%
CAAGAAGCTGTAAATGAGGT+TGG - tig0016634:3174-3193 Msa1359030:intron 40.0%
AAGCTGTAAATGAGGTTGGA+AGG - tig0016634:3178-3197 Msa1359030:intron 40.0%
AGCTGTAAATGAGGTTGGAA+GGG - tig0016634:3179-3198 Msa1359030:intron 40.0%
! CCTGAATTTTTCGCCAACAT+TGG + tig0016634:3267-3286 Msa1359030:intergenic 40.0%
CCAATGTTGGCGAAAAATTC+AGG - tig0016634:3264-3283 Msa1359030:intron 40.0%
GGCGAAAAATTCAGGAATCA+AGG - tig0016634:3272-3291 Msa1359030:intron 40.0%
TACAACGTTGTAGCTGTTGT+AGG - tig0016634:3537-3556 Msa1359030:intron 40.0%
TTTGTTGCAGATTTGCGATG+AGG - tig0016634:3916-3935 Msa1359030:CDS 40.0%
AGATTAAGAGTCATCCGTGG+TGG - tig0016634:2964-2983 Msa1359030:intron 45.0%
TTATGATCCGTCTGATGCGT+CGG - tig0016634:2998-3017 Msa1359030:intron 45.0%
AAATGAGGTTGGAAGGGTTC+TGG - tig0016634:3185-3204 Msa1359030:intron 45.0%
!! GAGTCGTTCGTTTGTTGTTG+CGG - tig0016634:3218-3237 Msa1359030:intron 45.0%
!! AGTCGTTCGTTTGTTGTTGC+GGG - tig0016634:3219-3238 Msa1359030:intron 45.0%
GTCTGAATTCGCTCCAATGT+TGG - tig0016634:3251-3270 Msa1359030:intron 45.0%
!! GGTGTTTTTGTGCCAGATAG+TGG - tig0016634:3297-3316 Msa1359030:intron 45.0%
!! TAGTGGCACAGAGAGCATTT+CGG - tig0016634:3314-3333 Msa1359030:intron 45.0%
CCTTGAGAAAGTAACTCAGC+CGG + tig0016634:3354-3373 Msa1359030:intergenic 45.0%
AGATTAAGAGTCATCCGTGG+TGG - tig0016634:2964-2983 Msa1359030:intron 45.0%
TTATGATCCGTCTGATGCGT+CGG - tig0016634:2998-3017 Msa1359030:intron 45.0%
AAATGAGGTTGGAAGGGTTC+TGG - tig0016634:3185-3204 Msa1359030:intron 45.0%
!! GAGTCGTTCGTTTGTTGTTG+CGG - tig0016634:3218-3237 Msa1359030:intron 45.0%
!! AGTCGTTCGTTTGTTGTTGC+GGG - tig0016634:3219-3238 Msa1359030:intron 45.0%
GTCTGAATTCGCTCCAATGT+TGG - tig0016634:3251-3270 Msa1359030:intron 45.0%
!! GGTGTTTTTGTGCCAGATAG+TGG - tig0016634:3297-3316 Msa1359030:intron 45.0%
!! TAGTGGCACAGAGAGCATTT+CGG - tig0016634:3314-3333 Msa1359030:intron 45.0%
CCTTGAGAAAGTAACTCAGC+CGG + tig0016634:3354-3373 Msa1359030:intergenic 45.0%
GACGGATCATAAACCCAACC+TGG + tig0016634:2990-3009 Msa1359030:intergenic 50.0%
! AAGGCTTCAATTGTGACGGG+TGG + tig0016634:3092-3111 Msa1359030:intergenic 50.0%
!! GTCGTTCGTTTGTTGTTGCG+GGG - tig0016634:3220-3239 Msa1359030:intron 50.0%
!! TCGTTCGTTTGTTGTTGCGG+GGG - tig0016634:3221-3240 Msa1359030:intron 50.0%
! ATGCTCTCTGTGCCACTATC+TGG + tig0016634:3312-3331 Msa1359030:intergenic 50.0%
TTCGGCGGTTACACTTGAAC+CGG - tig0016634:3332-3351 Msa1359030:intron 50.0%
! CCGGCTGAGTTACTTTCTCA+AGG - tig0016634:3351-3370 Msa1359030:intron 50.0%
GACGGATCATAAACCCAACC+TGG + tig0016634:2990-3009 Msa1359030:intergenic 50.0%
! AAGGCTTCAATTGTGACGGG+TGG + tig0016634:3092-3111 Msa1359030:intergenic 50.0%
!! GTCGTTCGTTTGTTGTTGCG+GGG - tig0016634:3220-3239 Msa1359030:intron 50.0%
!! TCGTTCGTTTGTTGTTGCGG+GGG - tig0016634:3221-3240 Msa1359030:intron 50.0%
! ATGCTCTCTGTGCCACTATC+TGG + tig0016634:3312-3331 Msa1359030:intergenic 50.0%
TTCGGCGGTTACACTTGAAC+CGG - tig0016634:3332-3351 Msa1359030:intron 50.0%
! CCGGCTGAGTTACTTTCTCA+AGG - tig0016634:3351-3370 Msa1359030:intron 50.0%
TAAACCCAACCTGGCCACCA+CGG + tig0016634:2981-3000 Msa1359030:intergenic 55.0%
GCAAGTTCCGACGCATCAGA+CGG + tig0016634:3008-3027 Msa1359030:intergenic 55.0%
!! TGGCACAGAGAGCATTTCGG+CGG - tig0016634:3317-3336 Msa1359030:intron 55.0%
TAAACCCAACCTGGCCACCA+CGG + tig0016634:2981-3000 Msa1359030:intergenic 55.0%
GCAAGTTCCGACGCATCAGA+CGG + tig0016634:3008-3027 Msa1359030:intergenic 55.0%
!! TGGCACAGAGAGCATTTCGG+CGG - tig0016634:3317-3336 Msa1359030:intron 55.0%
AAGAGTCATCCGTGGTGGCC+AGG - tig0016634:2969-2988 Msa1359030:intron 60.0%
TCATCCGTGGTGGCCAGGTT+GGG - tig0016634:2974-2993 Msa1359030:intron 60.0%
AAGAGTCATCCGTGGTGGCC+AGG - tig0016634:2969-2988 Msa1359030:intron 60.0%
TCATCCGTGGTGGCCAGGTT+GGG - tig0016634:2974-2993 Msa1359030:intron 60.0%
GTCATCCGTGGTGGCCAGGT+TGG - tig0016634:2973-2992 Msa1359030:intron 65.0%
GTCATCCGTGGTGGCCAGGT+TGG - tig0016634:2973-2992 Msa1359030:intron 65.0%
Chromosome Type Strat End Strand Name
tig0016634 gene 2922 3976 2922 ID=Msa1359030;Name=Msa1359030
tig0016634 mRNA 2922 3976 2922 ID=Msa1359030-mRNA-1;Parent=Msa1359030;Name=Msa1359030-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|0|0|0.66|1|1|3|0|117
tig0016634 exon 3908 3976 3908 ID=Msa1359030-mRNA-1:exon:11163;Parent=Msa1359030-mRNA-1
tig0016634 exon 3560 3790 3560 ID=Msa1359030-mRNA-1:exon:11162;Parent=Msa1359030-mRNA-1
tig0016634 exon 2922 2972 2922 ID=Msa1359030-mRNA-1:exon:11161;Parent=Msa1359030-mRNA-1
tig0016634 CDS 3908 3976 3908 ID=Msa1359030-mRNA-1:cds;Parent=Msa1359030-mRNA-1
tig0016634 CDS 3560 3790 3560 ID=Msa1359030-mRNA-1:cds;Parent=Msa1359030-mRNA-1
tig0016634 CDS 2922 2972 2922 ID=Msa1359030-mRNA-1:cds;Parent=Msa1359030-mRNA-1
Gene Sequence

>Msa1359030

ATGAAAGGTGTGACATTTCTGTTGGCGAATTTGTTTGGGGAAAGATTAAGAGTCATCCGTGGTGGCCAGCCTTTTAAGGATAATTTTGATGATATGGTGAGACGAGGTAGTTCCAAAGCTTTTACCTATGCTGTTCAAGAAGCTGTAAATGAGGTTGGAAGGGTTCTGGTTACGAAGATGAGTCGTTCGTTTGTTGTTGCGGGGGAGACTGAGTCTGAATTCGCTCCAATGTTGGCGAAAAATTCAGGAATCAAGGAAGGTGTTTTTGTGCCAGATAGTGGCACAGAGAGCATTTCGGCGATTTGCGATGAGGAATATGATAAGAATTCAAATATTCGTTCTGTTTCTGCT

Protein sequence

>Msa1359030

MKGVTFLLANLFGERLRVIRGGQPFKDNFDDMVRRGSSKAFTYAVQEAVNEVGRVLVTKMSRSFVVAGETESEFAPMLAKNSGIKEGVFVPDSGTESISAICDEEYDKNSNIRSVSA