AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0244810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0244810 MtrunA17_Chr4g0020841 95.455 154 7 0 1 154 323 476 4.76e-104 305
Msa0244810 MtrunA17_Chr2g0308441 94.156 154 9 0 1 154 343 496 6.14e-104 306
Msa0244810 MtrunA17_Chr4g0020821 95.425 153 7 0 1 153 529 681 5.43e-99 305
Msa0244810 MtrunA17_Chr2g0316961 94.667 75 3 1 80 154 1 74 5.46e-46 144
Msa0244810 MtrunA17_Chr2g0316951 93.939 66 4 0 1 66 205 270 2.63e-38 131
Msa0244810 MtrunA17_Chr7g0252871 34.234 111 73 0 20 130 411 521 1.53e-21 89.7
Msa0244810 MtrunA17_Chr8g0369501 32.639 144 90 3 5 145 378 517 4.41e-16 74.3
Msa0244810 MtrunA17_Chr8g0369501 27.329 161 105 4 1 154 304 459 6.70e-11 59.3
Msa0244810 MtrunA17_Chr3g0079211 27.692 130 88 3 1 126 331 458 2.29e-14 69.3
Msa0244810 MtrunA17_Chr6g0480621 31.707 123 79 2 1 120 414 534 3.59e-14 68.9
Msa0244810 MtrunA17_Chr2g0284291 30.894 123 80 2 1 120 440 560 7.98e-13 64.7
Msa0244810 MtrunA17_Chr3g0136851 31.061 132 86 3 12 140 891 1020 4.50e-12 62.8
Msa0244810 MtrunA17_Chr2g0333551 30.435 115 79 1 13 127 361 474 5.51e-12 62.4
Msa0244810 MtrunA17_Chr8g0368871 28.358 134 89 3 20 148 78 209 3.17e-11 59.3
Msa0244810 MtrunA17_Chr8g0368891 29.104 134 88 3 20 148 519 650 6.17e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0244810 AT3G15200.1 52.713 129 61 0 2 130 369 497 2.00e-42 147
Msa0244810 AT5G15010.1 35.088 114 74 0 19 132 405 518 5.58e-20 85.9
Msa0244810 AT3G49730.1 33.071 127 80 2 1 124 358 482 8.76e-16 73.9
Msa0244810 AT3G62540.1 30.081 123 81 2 1 120 419 539 2.92e-13 66.6
Msa0244810 AT5G14820.1 30.081 123 81 2 1 120 418 538 3.01e-13 66.6
Msa0244810 AT3G62470.1 29.268 123 82 2 1 120 419 539 6.89e-13 65.5
Msa0244810 AT5G65820.1 27.972 143 98 2 1 140 373 513 6.43e-12 62.8
Msa0244810 AT1G80550.1 27.869 122 86 1 1 122 310 429 3.12e-11 60.5
Msa0244810 AT4G20090.2 29.371 143 94 3 1 140 203 341 7.22e-11 59.7
Msa0244810 AT4G20090.1 29.371 143 94 3 1 140 203 341 7.22e-11 59.7
Msa0244810 AT3G16010.1 26.619 139 98 2 2 137 214 351 9.41e-11 59.3

Find 48 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACTAGTTTCTCAGTTAT+TGG 0.185894 2_2:+23605296 None:intergenic
ATTCATGGACACTATGAAAA+TGG 0.201782 2_2:-23605380 Msa0244810:CDS
CTCCTTCAACTGACCGTTCA+TGG 0.241734 2_2:+23605271 None:intergenic
GGAAGAAGTTCCCACAGTTT+TGG 0.290985 2_2:-23605561 Msa0244810:CDS
AGGATATGGAGAGGAGGAGC+GGG 0.298048 2_2:-23605640 Msa0244810:CDS
TTATGAATTGGTAGAGGATA+TGG 0.306941 2_2:-23605654 Msa0244810:CDS
AATGGAGAAGGTTTATGAAT+TGG 0.342278 2_2:-23605666 Msa0244810:CDS
AGGATGCCATGCGTTACTTC+CGG 0.351196 2_2:-23605349 Msa0244810:CDS
GGATGCCATGCGTTACTTCC+GGG 0.379263 2_2:-23605348 Msa0244810:CDS
GAGCGATGATATCTGCAATT+TGG 0.379693 2_2:-23605510 Msa0244810:CDS
TAACTATAAGTAACACCATT+AGG 0.400430 2_2:+23605608 None:intergenic
TCGTATACCATAATGATTCA+TGG 0.404759 2_2:-23605395 Msa0244810:CDS
ATAGTGTCCATGAATCATTA+TGG 0.413440 2_2:+23605388 None:intergenic
TTGGAGAGGATGGAGAGGAT+TGG 0.437579 2_2:-23605542 Msa0244810:CDS
TCTTGAGGTTGTATATGAAA+TGG 0.451675 2_2:-23605487 Msa0244810:CDS
AAAACTGTGGGAACTTCTTC+CGG 0.455644 2_2:+23605563 None:intergenic
TTCCGGGAGATGACGTCTAA+AGG 0.461988 2_2:-23605332 Msa0244810:CDS
TTACTTGCTACAGTCGTTGA+AGG 0.463748 2_2:-23605588 Msa0244810:CDS
CTTGAGGTTGTATATGAAAT+GGG 0.475247 2_2:-23605486 Msa0244810:CDS
GAGGATATGGAGAGGAGGAG+CGG 0.476452 2_2:-23605641 Msa0244810:CDS
TTCCATGAACGGTCAGTTGA+AGG 0.478957 2_2:-23605273 Msa0244810:CDS
ATGAAATGGGATGATCTTGA+TGG 0.486117 2_2:-23605473 Msa0244810:CDS
TCCGGGAGATGACGTCTAAA+GGG 0.496057 2_2:-23605331 Msa0244810:CDS
TTCATGGACACTATGAAAAT+GGG 0.499216 2_2:-23605379 Msa0244810:CDS
GAAATGGAGAGGAATGGATT+GGG 0.500443 2_2:-23605431 Msa0244810:CDS
ATGGTATACGATCGTCGGTC+CGG 0.505274 2_2:+23605407 None:intergenic
TGAAATGGAGAGGAATGGAT+TGG 0.507563 2_2:-23605432 Msa0244810:CDS
AGTTTCTCAGTTATTGGCTC+CGG 0.514899 2_2:+23605302 None:intergenic
TGGGATGAAATGGAGAGGAA+TGG 0.515458 2_2:-23605437 Msa0244810:CDS
TCCCTTTAGACGTCATCTCC+CGG 0.519194 2_2:+23605330 None:intergenic
GAAGGTTTATGAATTGGTAG+AGG 0.530704 2_2:-23605660 Msa0244810:CDS
GGATATGGAGAGGAGGAGCG+GGG 0.537257 2_2:-23605639 Msa0244810:CDS
ATATCTGCAATTTGGTCTTG+AGG 0.543547 2_2:-23605502 Msa0244810:CDS
GGAGAGGAATGGATTGGGAC+CGG 0.546978 2_2:-23605426 Msa0244810:CDS
TGGTAGAGGATATGGAGAGG+AGG 0.551913 2_2:-23605646 Msa0244810:CDS
GTAGAGGCATCTAATGTTTG+AGG 0.568426 2_2:-23605221 None:intergenic
GCTACAGTCGTTGAAGGCAC+CGG 0.578516 2_2:-23605582 Msa0244810:CDS
TCCATCCTCTCCAAAACTGT+GGG 0.580902 2_2:+23605551 None:intergenic
ACAAGAAGGAGTTGAAGTAG+AGG 0.581060 2_2:-23605237 Msa0244810:CDS
CTCCATCCTCTCCAAAACTG+TGG 0.590036 2_2:+23605550 None:intergenic
AATTGGTAGAGGATATGGAG+AGG 0.597366 2_2:-23605649 Msa0244810:CDS
GACGTCTAAAGGGATAGTGC+CGG 0.603758 2_2:-23605321 Msa0244810:CDS
TCATTATGGTATACGATCGT+CGG 0.613969 2_2:+23605402 None:intergenic
AAACATGGGATGAAATGGAG+AGG 0.633586 2_2:-23605442 Msa0244810:CDS
CTATGAAAATGGGAGGACAA+AGG 0.642415 2_2:-23605369 Msa0244810:CDS
CATCTCCCGGAAGTAACGCA+TGG 0.648521 2_2:+23605343 None:intergenic
AAACTAGTGATTTCCATGAA+CGG 0.662348 2_2:-23605284 Msa0244810:CDS
ATGGACACTATGAAAATGGG+AGG 0.675142 2_2:-23605376 Msa0244810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAACTATAAGTAACACCATT+AGG + chr2_2:23605283-23605302 None:intergenic 25.0%
!!! TTGATGGATTGAAAAAAACA+TGG - chr2_2:23605431-23605450 Msa0244810:CDS 25.0%
!!! TGATGGATTGAAAAAAACAT+GGG - chr2_2:23605432-23605451 Msa0244810:CDS 25.0%
TTATGAATTGGTAGAGGATA+TGG - chr2_2:23605234-23605253 Msa0244810:CDS 30.0%
TCTTGAGGTTGTATATGAAA+TGG - chr2_2:23605401-23605420 Msa0244810:CDS 30.0%
CTTGAGGTTGTATATGAAAT+GGG - chr2_2:23605402-23605421 Msa0244810:CDS 30.0%
GAAAAAAACATGGGATGAAA+TGG - chr2_2:23605441-23605460 Msa0244810:CDS 30.0%
TCGTATACCATAATGATTCA+TGG - chr2_2:23605493-23605512 Msa0244810:CDS 30.0%
ATAGTGTCCATGAATCATTA+TGG + chr2_2:23605503-23605522 None:intergenic 30.0%
ATTCATGGACACTATGAAAA+TGG - chr2_2:23605508-23605527 Msa0244810:CDS 30.0%
TTCATGGACACTATGAAAAT+GGG - chr2_2:23605509-23605528 Msa0244810:CDS 30.0%
! ATCACTAGTTTCTCAGTTAT+TGG + chr2_2:23605595-23605614 None:intergenic 30.0%
AAACTAGTGATTTCCATGAA+CGG - chr2_2:23605604-23605623 Msa0244810:CDS 30.0%
GAAGGTTTATGAATTGGTAG+AGG - chr2_2:23605228-23605247 Msa0244810:CDS 35.0%
!! ATATCTGCAATTTGGTCTTG+AGG - chr2_2:23605386-23605405 Msa0244810:CDS 35.0%
ATGAAATGGGATGATCTTGA+TGG - chr2_2:23605415-23605434 Msa0244810:CDS 35.0%
TCATTATGGTATACGATCGT+CGG + chr2_2:23605489-23605508 None:intergenic 35.0%
GAGAGAACAGAAAAACAAGA+AGG - chr2_2:23605637-23605656 Msa0244810:CDS 35.0%
AATTGGTAGAGGATATGGAG+AGG - chr2_2:23605239-23605258 Msa0244810:CDS 40.0%
! TTACTTGCTACAGTCGTTGA+AGG - chr2_2:23605300-23605319 Msa0244810:CDS 40.0%
AAAACTGTGGGAACTTCTTC+CGG + chr2_2:23605328-23605347 None:intergenic 40.0%
GAGCGATGATATCTGCAATT+TGG - chr2_2:23605378-23605397 Msa0244810:CDS 40.0%
AAACATGGGATGAAATGGAG+AGG - chr2_2:23605446-23605465 Msa0244810:CDS 40.0%
TGAAATGGAGAGGAATGGAT+TGG - chr2_2:23605456-23605475 Msa0244810:CDS 40.0%
! GAAATGGAGAGGAATGGATT+GGG - chr2_2:23605457-23605476 Msa0244810:CDS 40.0%
ATGGACACTATGAAAATGGG+AGG - chr2_2:23605512-23605531 Msa0244810:CDS 40.0%
CTATGAAAATGGGAGGACAA+AGG - chr2_2:23605519-23605538 Msa0244810:CDS 40.0%
!! AGTTTCTCAGTTATTGGCTC+CGG + chr2_2:23605589-23605608 None:intergenic 40.0%
ACAAGAAGGAGTTGAAGTAG+AGG - chr2_2:23605651-23605670 Msa0244810:CDS 40.0%
! AGCGGGGATTGTTTTCCTAA+TGG - chr2_2:23605265-23605284 Msa0244810:CDS 45.0%
! GGAAGAAGTTCCCACAGTTT+TGG - chr2_2:23605327-23605346 Msa0244810:CDS 45.0%
!!! AAGTTCCCACAGTTTTGGAG+AGG - chr2_2:23605332-23605351 Msa0244810:CDS 45.0%
TCCATCCTCTCCAAAACTGT+GGG + chr2_2:23605340-23605359 None:intergenic 45.0%
TGGGATGAAATGGAGAGGAA+TGG - chr2_2:23605451-23605470 Msa0244810:CDS 45.0%
TTCCATGAACGGTCAGTTGA+AGG - chr2_2:23605615-23605634 Msa0244810:CDS 45.0%
TGGTAGAGGATATGGAGAGG+AGG - chr2_2:23605242-23605261 Msa0244810:CDS 50.0%
!!! TCCCACAGTTTTGGAGAGGA+TGG - chr2_2:23605336-23605355 Msa0244810:CDS 50.0%
CTCCATCCTCTCCAAAACTG+TGG + chr2_2:23605341-23605360 None:intergenic 50.0%
!!! CAGTTTTGGAGAGGATGGAG+AGG - chr2_2:23605341-23605360 Msa0244810:CDS 50.0%
TTGGAGAGGATGGAGAGGAT+TGG - chr2_2:23605346-23605365 Msa0244810:CDS 50.0%
ATGGTATACGATCGTCGGTC+CGG + chr2_2:23605484-23605503 None:intergenic 50.0%
AGGATGCCATGCGTTACTTC+CGG - chr2_2:23605539-23605558 Msa0244810:CDS 50.0%
TTCCGGGAGATGACGTCTAA+AGG - chr2_2:23605556-23605575 Msa0244810:CDS 50.0%
TCCGGGAGATGACGTCTAAA+GGG - chr2_2:23605557-23605576 Msa0244810:CDS 50.0%
TCCCTTTAGACGTCATCTCC+CGG + chr2_2:23605561-23605580 None:intergenic 50.0%
GACGTCTAAAGGGATAGTGC+CGG - chr2_2:23605567-23605586 Msa0244810:CDS 50.0%
CTCCTTCAACTGACCGTTCA+TGG + chr2_2:23605620-23605639 None:intergenic 50.0%
GAGGATATGGAGAGGAGGAG+CGG - chr2_2:23605247-23605266 Msa0244810:CDS 55.0%
AGGATATGGAGAGGAGGAGC+GGG - chr2_2:23605248-23605267 Msa0244810:CDS 55.0%
! GCTACAGTCGTTGAAGGCAC+CGG - chr2_2:23605306-23605325 Msa0244810:CDS 55.0%
!! GGAGAGGAATGGATTGGGAC+CGG - chr2_2:23605462-23605481 Msa0244810:CDS 55.0%
GGATGCCATGCGTTACTTCC+GGG - chr2_2:23605540-23605559 Msa0244810:CDS 55.0%
CATCTCCCGGAAGTAACGCA+TGG + chr2_2:23605548-23605567 None:intergenic 55.0%
GGATATGGAGAGGAGGAGCG+GGG - chr2_2:23605249-23605268 Msa0244810:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 23605223 23605687 23605223 ID=Msa0244810;Name=Msa0244810
chr2_2 mRNA 23605223 23605687 23605223 ID=Msa0244810-mRNA-1;Parent=Msa0244810;Name=Msa0244810-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|154
chr2_2 exon 23605223 23605687 23605223 ID=Msa0244810-mRNA-1:exon:9053;Parent=Msa0244810-mRNA-1
chr2_2 CDS 23605223 23605687 23605223 ID=Msa0244810-mRNA-1:cds;Parent=Msa0244810-mRNA-1
Gene Sequence

>Msa0244810

ATGGAGAAGGTTTATGAATTGGTAGAGGATATGGAGAGGAGGAGCGGGGATTGTTTTCCTAATGGTGTTACTTATAGTTACTTGCTACAGTCGTTGAAGGCACCGGAAGAAGTTCCCACAGTTTTGGAGAGGATGGAGAGGATTGGATGTGCAATGAGCGATGATATCTGCAATTTGGTCTTGAGGTTGTATATGAAATGGGATGATCTTGATGGATTGAAAAAAACATGGGATGAAATGGAGAGGAATGGATTGGGACCGGACCGACGATCGTATACCATAATGATTCATGGACACTATGAAAATGGGAGGACAAAGGATGCCATGCGTTACTTCCGGGAGATGACGTCTAAAGGGATAGTGCCGGAGCCAATAACTGAGAAACTAGTGATTTCCATGAACGGTCAGTTGAAGGAGAGAACAGAAAAACAAGAAGGAGTTGAAGTAGAGGCATCTAATGTTTGA

Protein sequence

>Msa0244810

MEKVYELVEDMERRSGDCFPNGVTYSYLLQSLKAPEEVPTVLERMERIGCAMSDDICNLVLRLYMKWDDLDGLKKTWDEMERNGLGPDRRSYTIMIHGHYENGRTKDAMRYFREMTSKGIVPEPITEKLVISMNGQLKERTEKQEGVEVEASNV*