AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1451190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451190 MtrunA17_Chr3g0131781 78.102 137 8 2 1 115 1 137 1.09e-62 192
Msa1451190 MtrunA17_Chr8g0385941 41.916 167 40 9 1 116 1 161 6.96e-21 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCAAACCAAATACAATT+AGG 0.178192 tig0033260:-11945 None:intergenic
CCAATTCGATCTTGGTCTTT+TGG 0.187350 tig0033260:-11595 None:intergenic
TGAACACCCCTAATTGTATT+TGG 0.203872 tig0033260:+11937 Msa1451190:CDS
TGGTAGCTGGAAAGTGAAAA+AGG 0.224871 tig0033260:+11569 Msa1451190:CDS
TCGTTGCTCCGCCGTTTATT+TGG 0.233205 tig0033260:-11304 None:intergenic
GTTCAAACCAAATACAATTA+GGG 0.300309 tig0033260:-11944 None:intergenic
AACACCGACGGTGTAATTAT+TGG 0.331686 tig0033260:+11549 Msa1451190:CDS
CCTCTTTCTTGATGTGCATT+GGG 0.358724 tig0033260:-11985 None:intergenic
CAAAAGACCAAGATCGAATT+GGG 0.359194 tig0033260:+11596 Msa1451190:CDS
GCCTCTTTCTTGATGTGCAT+TGG 0.361842 tig0033260:-11986 None:intergenic
CCAAAAGACCAAGATCGAAT+TGG 0.364115 tig0033260:+11595 Msa1451190:CDS
AAATCATGGCGATTCGGCTT+CGG 0.384660 tig0033260:+11443 Msa1451190:CDS
CCGCCGTTTATTTGGACCTT+CGG 0.398024 tig0033260:-11296 None:intergenic
GGTCTCCGATGTCGCCTAAT+CGG 0.413080 tig0033260:-11388 None:intergenic
TTCGGCTTCGGCAACGTCTC+AGG 0.441191 tig0033260:+11455 Msa1451190:CDS
TTCATTGTTCTAAACTGCAT+TGG 0.445305 tig0033260:-11918 None:intergenic
CCACCGAAGGTCCAAATAAA+CGG 0.453209 tig0033260:+11293 Msa1451190:CDS
GAAGAAACCCAATTCGATCT+TGG 0.472216 tig0033260:-11603 None:intergenic
TTCGATCCGTGAGGTTCTGA+AGG 0.475990 tig0033260:-11344 None:intergenic
AGTTCAAAATCATGGCGATT+CGG 0.480998 tig0033260:+11437 Msa1451190:CDS
CGCCTAATCGGACGCTGGGT+CGG 0.497118 tig0033260:-11376 None:intergenic
GCGGCGAGGGTGGGAATCAG+AGG 0.498456 tig0033260:-11414 None:intergenic
CCCAATGCACATCAAGAAAG+AGG 0.499995 tig0033260:+11985 Msa1451190:CDS
CTCGCCGCAGTTCAAAATCA+TGG 0.500043 tig0033260:+11429 Msa1451190:CDS
AATAATTACACCGTCGGTGT+TGG 0.505458 tig0033260:-11547 None:intergenic
GTTTCTTCGCGAATTGAAGA+AGG 0.522515 tig0033260:+11618 Msa1451190:CDS
TTTATTTGGACCTTCGGTGG+CGG 0.523272 tig0033260:-11290 None:intergenic
GATGTCGCCTAATCGGACGC+TGG 0.529379 tig0033260:-11381 None:intergenic
ACGGTGTAATTATTGGTAGC+TGG 0.534282 tig0033260:+11556 Msa1451190:CDS
ATGTCGCCTAATCGGACGCT+GGG 0.537206 tig0033260:-11380 None:intergenic
CCGTTTATTTGGACCTTCGG+TGG 0.544420 tig0033260:-11293 None:intergenic
TCGATCCGTGAGGTTCTGAA+GGG 0.559702 tig0033260:-11343 None:intergenic
CACCGACCCAGCGTCCGATT+AGG 0.565703 tig0033260:+11374 Msa1451190:CDS
CGGCGAGGGTGGGAATCAGA+GGG 0.591797 tig0033260:-11413 None:intergenic
GGAAGAGGTTCCGCCACCGA+AGG 0.594303 tig0033260:+11280 Msa1451190:CDS
GAGGGTGGGAATCAGAGGGT+TGG 0.602586 tig0033260:-11409 None:intergenic
GAAACGGAAGAAAATGGAAG+AGG 0.606203 tig0033260:+11265 None:intergenic
GTGCATTGGGATGCAAAAGA+TGG 0.609464 tig0033260:-11972 None:intergenic
CGATCCGTGAGGTTCTGAAG+GGG 0.626502 tig0033260:-11342 None:intergenic
TTCAAACCAAATACAATTAG+GGG 0.626752 tig0033260:-11943 None:intergenic
AGCGTCCGATTAGGCGACAT+CGG 0.639876 tig0033260:+11383 Msa1451190:CDS
GATCCGTGAGGTTCTGAAGG+GGG 0.654909 tig0033260:-11341 None:intergenic
GCTACCAATAATTACACCGT+CGG 0.667969 tig0033260:-11553 None:intergenic
GACGTAGAAACCAACACCGA+CGG 0.675808 tig0033260:+11537 Msa1451190:intron
CAGAACCTCACGGATCGAAG+CGG 0.686667 tig0033260:+11348 Msa1451190:CDS
TGTTGCCGCTTCGATCCGTG+AGG 0.714492 tig0033260:-11353 None:intergenic
CCGAAGGTCCAAATAAACGG+CGG 0.735932 tig0033260:+11296 Msa1451190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAACCAGTTTTTTTTAAAG+TGG + tig0033260:11856-11875 Msa1451190:intron 20.0%
!!! AAAACCACTTTAAAAAAAAC+TGG - tig0033260:11863-11882 None:intergenic 20.0%
!!! AGATTTTGAAGGTGATAATT+CGG + tig0033260:11674-11693 Msa1451190:intron 25.0%
! AACCAGTTCAAAAAAAAATC+TGG + tig0033260:11706-11725 Msa1451190:intron 25.0%
!!! AACCAGATTTTTTTTTGAAC+TGG - tig0033260:11711-11730 None:intergenic 25.0%
! CTGGTTAAAAATAAGTAAAC+CGG + tig0033260:11725-11744 Msa1451190:intron 25.0%
! TTCAAACCAAATACAATTAG+GGG - tig0033260:11946-11965 None:intergenic 25.0%
! GTTCAAACCAAATACAATTA+GGG - tig0033260:11947-11966 None:intergenic 25.0%
! TCCTTTGACTAATTTGACTT+CGG + tig0033260:11496-11515 Msa1451190:intron 30.0%
! GATTTGTATCGAGATTTTGA+AGG + tig0033260:11663-11682 Msa1451190:CDS 30.0%
!! GATTTTGAAGGTGATAATTC+GGG + tig0033260:11675-11694 Msa1451190:intron 30.0%
! ACCGGTTTAAAACTATAAAC+CGG + tig0033260:11743-11762 Msa1451190:intron 30.0%
!!! ACCGGTTTATAGTTTTAAAC+CGG - tig0033260:11747-11766 None:intergenic 30.0%
!!! CCGGTTTATTTATTTTGAAC+CGG - tig0033260:11776-11795 None:intergenic 30.0%
CCGGTTCAAAATAAATAAAC+CGG + tig0033260:11773-11792 Msa1451190:intron 30.0%
! TTTTTAAACCAGTTCCAGTT+CGG - tig0033260:11835-11854 None:intergenic 30.0%
CTGAATTGTATCCATTAATG+TGG + tig0033260:11888-11907 Msa1451190:intron 30.0%
! TTCATTGTTCTAAACTGCAT+TGG - tig0033260:11921-11940 None:intergenic 30.0%
CGTTCAAACCAAATACAATT+AGG - tig0033260:11948-11967 None:intergenic 30.0%
AGTTCAAAATCATGGCGATT+CGG + tig0033260:11437-11456 Msa1451190:CDS 35.0%
ACCGAAGTCAAATTAGTCAA+AGG - tig0033260:11500-11519 None:intergenic 35.0%
CAAAAGACCAAGATCGAATT+GGG + tig0033260:11596-11615 Msa1451190:CDS 35.0%
TTGGAACTGAACCACATTAA+TGG - tig0033260:11902-11921 None:intergenic 35.0%
TGAACACCCCTAATTGTATT+TGG + tig0033260:11937-11956 Msa1451190:CDS 35.0%
! ATCGCCATGATTTTGAACTG+CGG - tig0033260:11436-11455 None:intergenic 40.0%
TCTACGTCTTCCATCGAAAT+CGG - tig0033260:11525-11544 None:intergenic 40.0%
AATAATTACACCGTCGGTGT+TGG - tig0033260:11550-11569 None:intergenic 40.0%
AACACCGACGGTGTAATTAT+TGG + tig0033260:11549-11568 Msa1451190:CDS 40.0%
GCTACCAATAATTACACCGT+CGG - tig0033260:11556-11575 None:intergenic 40.0%
! ACGGTGTAATTATTGGTAGC+TGG + tig0033260:11556-11575 Msa1451190:CDS 40.0%
TGGTAGCTGGAAAGTGAAAA+AGG + tig0033260:11569-11588 Msa1451190:CDS 40.0%
GGTAGCTGGAAAGTGAAAAA+GGG + tig0033260:11570-11589 Msa1451190:CDS 40.0%
!! CCAATTCGATCTTGGTCTTT+TGG - tig0033260:11598-11617 None:intergenic 40.0%
CCAAAAGACCAAGATCGAAT+TGG + tig0033260:11595-11614 Msa1451190:CDS 40.0%
GAAGAAACCCAATTCGATCT+TGG - tig0033260:11606-11625 None:intergenic 40.0%
GTTTCTTCGCGAATTGAAGA+AGG + tig0033260:11618-11637 Msa1451190:CDS 40.0%
!! ACTATAAACCGGTTCGAAAC+CGG + tig0033260:11754-11773 Msa1451190:intron 40.0%
!! ATTTTGAACCGGTTTCGAAC+CGG - tig0033260:11765-11784 None:intergenic 40.0%
! ACCGGTTCAAAACTGTAAAC+CGG + tig0033260:11791-11810 Msa1451190:intron 40.0%
!!! ACCGGTTTACAGTTTTGAAC+CGG - tig0033260:11795-11814 None:intergenic 40.0%
CCTCTTTCTTGATGTGCATT+GGG - tig0033260:11988-12007 None:intergenic 40.0%
TTTATTTGGACCTTCGGTGG+CGG - tig0033260:11293-11312 None:intergenic 45.0%
CCACCGAAGGTCCAAATAAA+CGG + tig0033260:11293-11312 Msa1451190:CDS 45.0%
! ATGATTTTGAACTGCGGCGA+GGG - tig0033260:11430-11449 None:intergenic 45.0%
! AAATCATGGCGATTCGGCTT+CGG + tig0033260:11443-11462 Msa1451190:CDS 45.0%
GTCAAAGGACGAGTCTTCAA+GGG - tig0033260:11485-11504 None:intergenic 45.0%
AGTCAAAGGACGAGTCTTCA+AGG - tig0033260:11486-11505 None:intergenic 45.0%
ACTTCGGTTTCCGATTTCGA+TGG + tig0033260:11512-11531 Msa1451190:intron 45.0%
!! ACTGTAAACCGGTTCTGAAC+CGG + tig0033260:11802-11821 Msa1451190:intron 45.0%
! GTTTAGAACCGGTTCAGAAC+CGG - tig0033260:11813-11832 None:intergenic 45.0%
! GTTCCAGTTCGGTTTAGAAC+CGG - tig0033260:11824-11843 None:intergenic 45.0%
! GTTCTAAACCGAACTGGAAC+TGG + tig0033260:11824-11843 Msa1451190:intron 45.0%
GTGCATTGGGATGCAAAAGA+TGG - tig0033260:11975-11994 None:intergenic 45.0%
CCCAATGCACATCAAGAAAG+AGG + tig0033260:11985-12004 Msa1451190:CDS 45.0%
GCCTCTTTCTTGATGTGCAT+TGG - tig0033260:11989-12008 None:intergenic 45.0%
CCGTTTATTTGGACCTTCGG+TGG - tig0033260:11296-11315 None:intergenic 50.0%
CCGCCGTTTATTTGGACCTT+CGG - tig0033260:11299-11318 None:intergenic 50.0%
CCGAAGGTCCAAATAAACGG+CGG + tig0033260:11296-11315 Msa1451190:CDS 50.0%
TCGTTGCTCCGCCGTTTATT+TGG - tig0033260:11307-11326 None:intergenic 50.0%
!! TCGATCCGTGAGGTTCTGAA+GGG - tig0033260:11346-11365 None:intergenic 50.0%
! TTCGATCCGTGAGGTTCTGA+AGG - tig0033260:11347-11366 None:intergenic 50.0%
! CATGATTTTGAACTGCGGCG+AGG - tig0033260:11431-11450 None:intergenic 50.0%
CTCGCCGCAGTTCAAAATCA+TGG + tig0033260:11429-11448 Msa1451190:CDS 50.0%
GACGTAGAAACCAACACCGA+CGG + tig0033260:11537-11556 Msa1451190:intron 50.0%
!! GAACCGGTTCTAAACCGAAC+TGG + tig0033260:11818-11837 Msa1451190:intron 50.0%
CTACCCCCTTCAGAACCTCA+CGG + tig0033260:11338-11357 Msa1451190:CDS 55.0%
!! GATCCGTGAGGTTCTGAAGG+GGG - tig0033260:11344-11363 None:intergenic 55.0%
!! CGATCCGTGAGGTTCTGAAG+GGG - tig0033260:11345-11364 None:intergenic 55.0%
CAGAACCTCACGGATCGAAG+CGG + tig0033260:11348-11367 Msa1451190:CDS 55.0%
ATGTCGCCTAATCGGACGCT+GGG - tig0033260:11383-11402 None:intergenic 55.0%
AGCGTCCGATTAGGCGACAT+CGG + tig0033260:11383-11402 Msa1451190:CDS 55.0%
GGTCTCCGATGTCGCCTAAT+CGG - tig0033260:11391-11410 None:intergenic 55.0%
! TTTTGAACTGCGGCGAGGGT+GGG - tig0033260:11426-11445 None:intergenic 55.0%
! ATTTTGAACTGCGGCGAGGG+TGG - tig0033260:11427-11446 None:intergenic 55.0%
! TGTTGCCGCTTCGATCCGTG+AGG - tig0033260:11356-11375 None:intergenic 60.0%
GATGTCGCCTAATCGGACGC+TGG - tig0033260:11384-11403 None:intergenic 60.0%
GAGGGTGGGAATCAGAGGGT+TGG - tig0033260:11412-11431 None:intergenic 60.0%
TTCGGCTTCGGCAACGTCTC+AGG + tig0033260:11455-11474 Msa1451190:CDS 60.0%
GGAAGAGGTTCCGCCACCGA+AGG + tig0033260:11280-11299 Msa1451190:CDS 65.0%
CACCGACCCAGCGTCCGATT+AGG + tig0033260:11374-11393 Msa1451190:CDS 65.0%
CGCCTAATCGGACGCTGGGT+CGG - tig0033260:11379-11398 None:intergenic 65.0%
CGGCGAGGGTGGGAATCAGA+GGG - tig0033260:11416-11435 None:intergenic 65.0%
GCGGCGAGGGTGGGAATCAG+AGG - tig0033260:11417-11436 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0033260 gene 11278 12016 11278 ID=Msa1451190;Name=Msa1451190
tig0033260 mRNA 11278 12016 11278 ID=Msa1451190-mRNA-1;Parent=Msa1451190;Name=Msa1451190-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0|0|0.66|1|1|3|0|148
tig0033260 exon 11278 11476 11278 ID=Msa1451190-mRNA-1:exon:17878;Parent=Msa1451190-mRNA-1
tig0033260 exon 11538 11684 11538 ID=Msa1451190-mRNA-1:exon:17879;Parent=Msa1451190-mRNA-1
tig0033260 exon 11916 12016 11916 ID=Msa1451190-mRNA-1:exon:17880;Parent=Msa1451190-mRNA-1
tig0033260 CDS 11278 11476 11278 ID=Msa1451190-mRNA-1:cds;Parent=Msa1451190-mRNA-1
tig0033260 CDS 11538 11684 11538 ID=Msa1451190-mRNA-1:cds;Parent=Msa1451190-mRNA-1
tig0033260 CDS 11916 12016 11916 ID=Msa1451190-mRNA-1:cds;Parent=Msa1451190-mRNA-1
Gene Sequence

>Msa1451190

ATGGAAGAGGTTCCGCCACCGAAGGTCCAAATAAACGGCGGAGCAACGACTGCAGCAACACTACCCCCTTCAGAACCTCACGGATCGAAGCGGCAACACCGACCCAGCGTCCGATTAGGCGACATCGGAGACCAACCCTCTGATTCCCACCCTCGCCGCAGTTCAAAATCATGGCGATTCGGCTTCGGCAACGTCTCAGACGTAGAAACCAACACCGACGGTGTAATTATTGGTAGCTGGAAAGTGAAAAAGGGTTCCAAAAGACCAAGATCGAATTGGGTTTCTTCGCGAATTGAAGAAGGTGAAGATGTTAATAATGATGATGATTTGTATCGAGATTTTGAAGTTCCAATGCAGTTTAGAACAATGAACACCCCTAATTGTATTTGGTTTGAACGTTTACCATCTTTTGCATCCCAATGCACATCAAGAAAGAGGCTACAATAG

Protein sequence

>Msa1451190

MEEVPPPKVQINGGATTAATLPPSEPHGSKRQHRPSVRLGDIGDQPSDSHPRRSSKSWRFGFGNVSDVETNTDGVIIGSWKVKKGSKRPRSNWVSSRIEEGEDVNNDDDLYRDFEVPMQFRTMNTPNCIWFERLPSFASQCTSRKRLQ*