Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0854980 | AES77573.1 | 61.074 | 149 | 40 | 6 | 1 | 131 | 1 | 149 | 5.99e-29 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0854980 | G7L456 | 61.074 | 149 | 40 | 6 | 1 | 131 | 1 | 149 | 2.86e-29 | 113 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa0854980 | 0.821350 | 4.230972e-53 | -8.615850e-47 |
| Msa0102070 | Msa0854980 | 0.808375 | 3.193807e-50 | -8.615850e-47 |
| Msa0636170 | Msa0854980 | 0.813007 | 3.183060e-51 | -8.615850e-47 |
| Msa0726140 | Msa0854980 | 0.825818 | 3.808465e-54 | -8.615850e-47 |
| Msa0735080 | Msa0854980 | 0.833084 | 6.531927e-56 | -8.615850e-47 |
| Msa0738490 | Msa0854980 | 0.802436 | 5.615715e-49 | -8.615850e-47 |
| Msa0743810 | Msa0854980 | 0.859837 | 3.137248e-63 | -8.615850e-47 |
| Msa0745060 | Msa0854980 | 0.847728 | 9.623623e-60 | -8.615850e-47 |
| Msa0783160 | Msa0854980 | 0.850083 | 2.135882e-60 | -8.615850e-47 |
| Msa0787600 | Msa0854980 | 0.803092 | 4.110394e-49 | -8.615850e-47 |
| Msa0825860 | Msa0854980 | 0.854748 | 1.000943e-61 | -8.615850e-47 |
| Msa0833290 | Msa0854980 | 0.821308 | 4.325275e-53 | -8.615850e-47 |
| Msa0123810 | Msa0854980 | 0.869553 | 2.840086e-66 | -8.615850e-47 |
| Msa0145750 | Msa0854980 | 0.805458 | 1.322299e-49 | -8.615850e-47 |
| Msa0153910 | Msa0854980 | 0.817930 | 2.554362e-52 | -8.615850e-47 |
| Msa0227480 | Msa0854980 | 0.806143 | 9.490689e-50 | -8.615850e-47 |
| Msa0231000 | Msa0854980 | 0.829493 | 4.990645e-55 | -8.615850e-47 |
| Msa0232850 | Msa0854980 | 0.840828 | 6.870972e-58 | -8.615850e-47 |
| Msa0366580 | Msa0854980 | 0.807944 | 3.946042e-50 | -8.615850e-47 |
| Msa0384800 | Msa0854980 | 0.824533 | 7.667244e-54 | -8.615850e-47 |
| Msa0414410 | Msa0854980 | 0.806646 | 7.433546e-50 | -8.615850e-47 |
| Msa0414420 | Msa0854980 | 0.835774 | 1.379952e-56 | -8.615850e-47 |
| Msa0436780 | Msa0854980 | 0.804706 | 1.899600e-49 | -8.615850e-47 |
| Msa0451730 | Msa0854980 | 0.802481 | 5.496666e-49 | -8.615850e-47 |
| Msa0452900 | Msa0854980 | 0.853663 | 2.059589e-61 | -8.615850e-47 |
| Msa0453120 | Msa0854980 | 0.819011 | 1.452633e-52 | -8.615850e-47 |
| Msa0454910 | Msa0854980 | 0.844595 | 6.859774e-59 | -8.615850e-47 |
| Msa0267000 | Msa0854980 | 0.854483 | 1.194612e-61 | -8.615850e-47 |
| Msa0267010 | Msa0854980 | 0.853043 | 3.101703e-61 | -8.615850e-47 |
| Msa0270520 | Msa0854980 | 0.822946 | 1.804835e-53 | -8.615850e-47 |
| Msa0309020 | Msa0854980 | 0.802417 | 5.666718e-49 | -8.615850e-47 |
| Msa0342240 | Msa0854980 | 0.836315 | 1.005593e-56 | -8.615850e-47 |
| Msa0360510 | Msa0854980 | 0.810414 | 1.166726e-50 | -8.615850e-47 |
| Msa0506270 | Msa0854980 | 0.822574 | 2.202086e-53 | -8.615850e-47 |
| Msa0564890 | Msa0854980 | 0.813781 | 2.151635e-51 | -8.615850e-47 |
| Msa0596150 | Msa0854980 | 0.802526 | 5.379633e-49 | -8.615850e-47 |
| Msa0846210 | Msa0854980 | 0.813746 | 2.189772e-51 | -8.615850e-47 |
| Msa0846330 | Msa0854980 | 0.812839 | 3.464010e-51 | -8.615850e-47 |
| Msa0846350 | Msa0854980 | 0.807073 | 6.040100e-50 | -8.615850e-47 |
| Msa0854980 | Msa0865320 | 0.837721 | 4.396816e-57 | -8.615850e-47 |
| Msa0854980 | Msa0872230 | 0.812594 | 3.920206e-51 | -8.615850e-47 |
| Msa0854980 | Msa0885290 | 0.825511 | 4.504917e-54 | -8.615850e-47 |
| Msa0854980 | Msa0888370 | 0.808244 | 3.405934e-50 | -8.615850e-47 |
| Msa0854980 | Msa0957860 | 0.834575 | 2.769097e-56 | -8.615850e-47 |
| Msa0854980 | Msa0957960 | 0.834597 | 2.733512e-56 | -8.615850e-47 |
| Msa0854980 | Msa0989060 | 0.841505 | 4.562099e-58 | -8.615850e-47 |
| Msa0854980 | Msa1016280 | 0.836101 | 1.139765e-56 | -8.615850e-47 |
| Msa0854980 | Msa1024240 | 0.803034 | 4.226933e-49 | -8.615850e-47 |
| Msa0854980 | Msa1025360 | 0.848122 | 7.492581e-60 | -8.615850e-47 |
| Msa0854980 | Msa1025370 | 0.845805 | 3.228991e-59 | -8.615850e-47 |
| Msa0854980 | Msa1044830 | 0.843137 | 1.685795e-58 | -8.615850e-47 |
| Msa0854980 | Msa1044840 | 0.846108 | 2.672304e-59 | -8.615850e-47 |
| Msa0854980 | Msa1060840 | 0.815972 | 7.027437e-52 | -8.615850e-47 |
| Msa0854980 | Msa1092990 | 0.850096 | 2.117564e-60 | -8.615850e-47 |
| Msa0854980 | Msa1100980 | 0.867796 | 1.050787e-65 | -8.615850e-47 |
| Msa0854980 | Msa1109440 | 0.825864 | 3.714654e-54 | -8.615850e-47 |
| Msa0854980 | Msa1121200 | 0.800097 | 1.690862e-48 | -8.615850e-47 |
| Msa0854980 | Msa1135740 | 0.847290 | 1.269979e-59 | -8.615850e-47 |
| Msa0854980 | Msa1136090 | 0.828567 | 8.367005e-55 | -8.615850e-47 |
| Msa0854980 | Msa1146110 | 0.825275 | 5.121926e-54 | -8.615850e-47 |
| Msa0854980 | Msa1150370 | 0.815688 | 8.132676e-52 | -8.615850e-47 |
| Msa0854980 | Msa1157890 | 0.813512 | 2.464852e-51 | -8.615850e-47 |
| Msa0854980 | Msa1160670 | 0.800326 | 1.518938e-48 | -8.615850e-47 |
| Msa0854980 | Msa1173920 | 0.813444 | 2.551973e-51 | -8.615850e-47 |
| Msa0854980 | Msa1178340 | 0.813194 | 2.896081e-51 | -8.615850e-47 |
| Msa0854980 | Msa1182090 | 0.816698 | 4.836579e-52 | -8.615850e-47 |
| Msa0854980 | Msa1187940 | 0.817096 | 3.936888e-52 | -8.615850e-47 |
| Msa0854980 | Msa1187950 | 0.845382 | 4.206251e-59 | -8.615850e-47 |
| Msa0854980 | Msa1192640 | 0.823796 | 1.142205e-53 | -8.615850e-47 |
| Msa0854980 | Msa1230430 | 0.818550 | 1.848805e-52 | -8.615850e-47 |
| Msa0854980 | Msa1246880 | 0.845233 | 4.613430e-59 | -8.615850e-47 |
| Msa0854980 | Msa1269670 | 0.839215 | 1.810666e-57 | -8.615850e-47 |
| Msa0854980 | Msa1270230 | 0.835362 | 1.753925e-56 | -8.615850e-47 |
| Msa0854980 | Msa1270240 | 0.842313 | 2.790696e-58 | -8.615850e-47 |
| Msa0854980 | Msa1270270 | 0.833282 | 5.833631e-56 | -8.615850e-47 |
| Msa0854980 | Msa1273230 | 0.825898 | 3.646547e-54 | -8.615850e-47 |
| Msa0854980 | Msa1283330 | 0.845632 | 3.598880e-59 | -8.615850e-47 |
| Msa0854980 | Msa1285930 | 0.844691 | 6.464493e-59 | -8.615850e-47 |
| Msa0854980 | Msa1287800 | 0.839834 | 1.249960e-57 | -8.615850e-47 |
| Msa0854980 | Msa1287810 | 0.852419 | 4.674806e-61 | -8.615850e-47 |
| Msa0854980 | Msa1297210 | 0.806411 | 8.335549e-50 | -8.615850e-47 |
| Msa0854980 | Msa1307890 | 0.800688 | 1.281746e-48 | -8.615850e-47 |
| Msa0854980 | Msa1369430 | 0.802049 | 6.746895e-49 | -8.615850e-47 |
| Msa0854980 | Msa1386330 | 0.826533 | 2.575204e-54 | -8.615850e-47 |
| Msa0854980 | Msa1402790 | 0.835881 | 1.296175e-56 | -8.615850e-47 |
| Msa0854980 | Msa1404000 | 0.825205 | 5.321517e-54 | -8.615850e-47 |
| Msa0854980 | Msa1415310 | 0.834404 | 3.055960e-56 | -8.615850e-47 |
| Msa0854980 | Msa1422180 | 0.819458 | 1.149361e-52 | -8.615850e-47 |
| Msa0854980 | Msa1433120 | 0.807472 | 4.970362e-50 | -8.615850e-47 |
| Msa0854980 | Msa1451190 | 0.821436 | 4.042693e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0854980 | MtrunA17_Chr2g0308161 | 77.027 | 74 | 12 | 1 | 63 | 131 | 25 | 98 | 1.93e-23 | 87.4 |
| Msa0854980 | MtrunA17_Chr3g0100521 | 68.116 | 69 | 22 | 0 | 63 | 131 | 68 | 136 | 1.13e-17 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCCATTTCTTGAAGTT+GGG | 0.317722 | 5_4:-47996927 | None:intergenic |
| TGAATTTGAAGTGGACGATT+TGG | 0.365773 | 5_4:+47997438 | Msa0854980:CDS |
| TTTCAGCCATTTCTTGAAGT+TGG | 0.374775 | 5_4:-47996928 | None:intergenic |
| AAGCAACTGGATGTAGAAAC+TGG | 0.396116 | 5_4:+47996794 | Msa0854980:CDS |
| CAACTCATTAAAGTTTGTCT+TGG | 0.406514 | 5_4:-47997471 | None:intergenic |
| CTAGTCCCAACTTCAAGAAA+TGG | 0.411146 | 5_4:+47996922 | Msa0854980:intron |
| CCTCTTGAATTTCATCATCT+AGG | 0.428108 | 5_4:-47997402 | None:intergenic |
| ACTTCAAGAAATGGCTGAAA+AGG | 0.436697 | 5_4:+47996931 | Msa0854980:CDS |
| AGGATGTAGAGGAAAGCAAC+TGG | 0.521301 | 5_4:+47996781 | Msa0854980:CDS |
| GAATTTCTTGAGGACAACAT+AGG | 0.538039 | 5_4:+47997331 | Msa0854980:CDS |
| CGCATATGATGAATTTCTTG+AGG | 0.551161 | 5_4:+47997321 | Msa0854980:CDS |
| GAAAGGAGCGTGTGCAAGGT+CGG | 0.551663 | 5_4:+47996990 | Msa0854980:CDS |
| TCAAGAAATGGCTGAAAAGG+AGG | 0.558678 | 5_4:+47996934 | Msa0854980:CDS |
| GAAACTCGAAAAGGATGTAG+AGG | 0.574679 | 5_4:+47996770 | Msa0854980:CDS |
| ATGGGAAAGGAGCGTGTGCA+AGG | 0.581557 | 5_4:+47996986 | Msa0854980:CDS |
| CATCGATGATGAATTTGAAG+TGG | 0.587100 | 5_4:+47997429 | Msa0854980:CDS |
| GATAGGTGAGGAATTTCAAG+AGG | 0.592117 | 5_4:+47997294 | Msa0854980:CDS |
| TTTCTTGAGGACAACATAGG+AGG | 0.595607 | 5_4:+47997334 | Msa0854980:CDS |
| CCTAGATGATGAAATTCAAG+AGG | 0.596370 | 5_4:+47997402 | Msa0854980:CDS |
| TATAGATGATGAATTTCAAG+AGG | 0.603702 | 5_4:+47997375 | Msa0854980:CDS |
| CATAGGAGGTGAATTTCAAG+AGG | 0.648875 | 5_4:+47997348 | Msa0854980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTAAAAATTTAAAGTTA+CGG | + | chr5_4:47996828-47996847 | Msa0854980:intron | 10.0% |
| !! | AAATTGTGTTTAACTAATAT+AGG | + | chr5_4:47996852-47996871 | Msa0854980:intron | 15.0% |
| !! | AGTTGAAGAAACTTAAAAAT+TGG | + | chr5_4:47997108-47997127 | Msa0854980:intron | 20.0% |
| !!! | ATGTTTTTTGAAACTCGAAA+AGG | + | chr5_4:47996761-47996780 | Msa0854980:CDS | 25.0% |
| ! | TTGTGTTTAACTAATATAGG+CGG | + | chr5_4:47996855-47996874 | Msa0854980:intron | 25.0% |
| ! | TTGTAATGATAGAATTAGGA+AGG | + | chr5_4:47997132-47997151 | Msa0854980:intron | 25.0% |
| ! | TGTAATGATAGAATTAGGAA+GGG | + | chr5_4:47997133-47997152 | Msa0854980:intron | 25.0% |
| ! | CAAAACAAAATTCCTATTTG+CGG | + | chr5_4:47997163-47997182 | Msa0854980:intron | 25.0% |
| !!! | TTTTATTTTCATATGCAGCA+AGG | + | chr5_4:47997247-47997266 | Msa0854980:intron | 25.0% |
| ! | TATAGATGATGAATTTCAAG+AGG | + | chr5_4:47997375-47997394 | Msa0854980:CDS | 25.0% |
| !!! | GAAGCTTTTAAAGCTGTTAT+GGG | + | chr5_4:47996968-47996987 | Msa0854980:CDS | 30.0% |
| !!! | TTTTAAAGCTGTTATGGGAA+AGG | + | chr5_4:47996973-47996992 | Msa0854980:CDS | 30.0% |
| ! | TGGCTTGTAATGATAGAATT+AGG | + | chr5_4:47997128-47997147 | Msa0854980:intron | 30.0% |
| GATGTTGAAAAAGATGAGAT+AGG | + | chr5_4:47997277-47997296 | Msa0854980:CDS | 30.0% | |
| TTCAGCCATTTCTTGAAGTT+GGG | - | chr5_4:47996930-47996949 | None:intergenic | 35.0% | |
| TTTCAGCCATTTCTTGAAGT+TGG | - | chr5_4:47996931-47996950 | None:intergenic | 35.0% | |
| ACTTCAAGAAATGGCTGAAA+AGG | + | chr5_4:47996931-47996950 | Msa0854980:CDS | 35.0% | |
| !!! | CGAAGCTTTTAAAGCTGTTA+TGG | + | chr5_4:47996967-47996986 | Msa0854980:CDS | 35.0% |
| !!! | GCGTGTTTTACTTGTTGTTT+TGG | + | chr5_4:47997197-47997216 | Msa0854980:intron | 35.0% |
| TGAAAAAGATGAGATAGGTG+AGG | + | chr5_4:47997282-47997301 | Msa0854980:CDS | 35.0% | |
| CGCATATGATGAATTTCTTG+AGG | + | chr5_4:47997321-47997340 | Msa0854980:CDS | 35.0% | |
| GAATTTCTTGAGGACAACAT+AGG | + | chr5_4:47997331-47997350 | Msa0854980:CDS | 35.0% | |
| CCTCTTGAATTTCATCATCT+AGG | - | chr5_4:47997405-47997424 | None:intergenic | 35.0% | |
| CCTAGATGATGAAATTCAAG+AGG | + | chr5_4:47997402-47997421 | Msa0854980:CDS | 35.0% | |
| CATCGATGATGAATTTGAAG+TGG | + | chr5_4:47997429-47997448 | Msa0854980:CDS | 35.0% | |
| ! | TGAATTTGAAGTGGACGATT+TGG | + | chr5_4:47997438-47997457 | Msa0854980:CDS | 35.0% |
| GAAACTCGAAAAGGATGTAG+AGG | + | chr5_4:47996770-47996789 | Msa0854980:CDS | 40.0% | |
| AAGCAACTGGATGTAGAAAC+TGG | + | chr5_4:47996794-47996813 | Msa0854980:CDS | 40.0% | |
| CTAGTCCCAACTTCAAGAAA+TGG | + | chr5_4:47996922-47996941 | Msa0854980:intron | 40.0% | |
| TCAAGAAATGGCTGAAAAGG+AGG | + | chr5_4:47996934-47996953 | Msa0854980:CDS | 40.0% | |
| CTCAAAACAACACCGCAAAT+AGG | - | chr5_4:47997178-47997197 | None:intergenic | 40.0% | |
| GATAGGTGAGGAATTTCAAG+AGG | + | chr5_4:47997294-47997313 | Msa0854980:CDS | 40.0% | |
| TTTCTTGAGGACAACATAGG+AGG | + | chr5_4:47997334-47997353 | Msa0854980:CDS | 40.0% | |
| CATAGGAGGTGAATTTCAAG+AGG | + | chr5_4:47997348-47997367 | Msa0854980:CDS | 40.0% | |
| AGGATGTAGAGGAAAGCAAC+TGG | + | chr5_4:47996781-47996800 | Msa0854980:CDS | 45.0% | |
| ! | ATGGGAAAGGAGCGTGTGCA+AGG | + | chr5_4:47996986-47997005 | Msa0854980:CDS | 55.0% |
| ! | GAAAGGAGCGTGTGCAAGGT+CGG | + | chr5_4:47996990-47997009 | Msa0854980:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 47996761 | 47997486 | 47996761 | ID=Msa0854980;Name=Msa0854980 |
| chr5_4 | mRNA | 47996761 | 47997486 | 47996761 | ID=Msa0854980-mRNA-1;Parent=Msa0854980;Name=Msa0854980-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|1|1|3|0|131 |
| chr5_4 | exon | 47996761 | 47996820 | 47996761 | ID=Msa0854980-mRNA-1:exon:15149;Parent=Msa0854980-mRNA-1 |
| chr5_4 | exon | 47996926 | 47997039 | 47996926 | ID=Msa0854980-mRNA-1:exon:15150;Parent=Msa0854980-mRNA-1 |
| chr5_4 | exon | 47997265 | 47997486 | 47997265 | ID=Msa0854980-mRNA-1:exon:15151;Parent=Msa0854980-mRNA-1 |
| chr5_4 | CDS | 47996761 | 47996820 | 47996761 | ID=Msa0854980-mRNA-1:cds;Parent=Msa0854980-mRNA-1 |
| chr5_4 | CDS | 47996926 | 47997039 | 47996926 | ID=Msa0854980-mRNA-1:cds;Parent=Msa0854980-mRNA-1 |
| chr5_4 | CDS | 47997265 | 47997486 | 47997265 | ID=Msa0854980-mRNA-1:cds;Parent=Msa0854980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
