AlfalfaGEDB | MS.gene032599

M. sativa cultivar XinJiangDaYe / MS.gene032599

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032599.t1	XP_003618164.1	95.9	220	9	0	1	220	1	220	3.10E-115	424.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032599.t1	Q9FMA8	63.9	219	75	3	1	215	3	221	4.0e-71	269.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032599.t1	G7KQ17	95.9	220	9	0	1	220	1	220	2.2e-115	424.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene032598	MS.gene032599	PPI
MS.gene032599	MS.gene82857	PPI
MS.gene032599	MS.gene037516	PPI
MS.gene032599	MS.gene032596	PPI
MS.gene032599	MS.gene037519	PPI
MS.gene032599	MS.gene70064	PPI
MS.gene032599	MS.gene047464	PPI
MS.gene032599	MS.gene82860	PPI
MS.gene032599	MS.gene005041	PPI
MS.gene032599	MS.gene82859	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032599.t1	MTR_6g005330	95.909	220	9	0	1	220	1	220	5.92e-155	428
MS.gene032599.t1	MTR_6g005360	94.091	220	13	0	1	220	1	220	8.35e-152	420
MS.gene032599.t1	MTR_6g005310	94.064	219	13	0	1	219	1	219	1.60e-150	417
MS.gene032599.t1	MTR_6g005340	93.636	220	14	0	1	220	1	220	2.12e-150	417
MS.gene032599.t1	MTR_1g079490	80.000	220	41	3	4	220	3	222	1.82e-124	351
MS.gene032599.t1	MTR_4g017040	75.566	221	53	1	1	220	1	221	2.04e-119	338
MS.gene032599.t1	MTR_2g019250	77.477	222	47	3	1	220	1	221	2.71e-119	338
MS.gene032599.t1	MTR_4g017050	77.027	222	48	3	1	220	1	221	3.52e-119	338
MS.gene032599.t1	MTR_5g046410	77.027	222	48	3	1	220	1	221	4.06e-119	338
MS.gene032599.t1	MTR_5g046430	77.027	222	48	3	1	220	1	221	4.06e-119	338

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032599.t1	AT5G39120	63.889	216	75	2	3	215	4	219	4.22e-93	272
MS.gene032599.t1	AT5G39150	63.426	216	76	2	3	215	4	219	3.23e-92	270
MS.gene032599.t1	AT5G39180	63.426	216	76	2	3	215	4	219	6.44e-92	269
MS.gene032599.t1	AT5G38940	66.837	196	63	1	22	215	26	221	2.73e-91	267
MS.gene032599.t1	AT5G38940	66.837	196	63	1	22	215	50	245	4.84e-91	268
MS.gene032599.t1	AT5G39110	64.055	217	74	3	2	215	5	220	7.38e-91	266
MS.gene032599.t1	AT5G38930	67.172	198	63	1	20	215	24	221	1.18e-90	266
MS.gene032599.t1	AT5G39160	66.834	199	62	3	20	215	22	219	6.65e-90	264
MS.gene032599.t1	AT5G39190	66.834	199	62	3	20	215	22	219	1.16e-89	263
MS.gene032599.t1	AT3G05950	63.964	222	72	5	1	215	1	221	2.62e-89	263

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Find 44 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAGATGCCAATGCTAAGA+AGG	0.503312	6.3:+1434638	None:intergenic
ACTATTGCTAATGCTGTATT+TGG	0.046653	6.3:-1433953	MS.gene032599:CDS
AGAACTTCTGAAGAGATCTT+TGG	0.268181	6.3:+1433923	None:intergenic
AGCAAATGATTTCTTCTACT+CGG	0.553402	6.3:-1434329	MS.gene032599:CDS
AGGGCTAGGATCAAATGCAA+AGG	0.662548	6.3:+1434614	None:intergenic
AGTATACATACCACCGGTCT+TGG	0.500629	6.3:+1434560	None:intergenic
ATAAGAATCTCAGTAGCTCT+AGG	0.469422	6.3:+1434163	None:intergenic
ATACTAGGACTAAACACTCT+AGG	0.572011	6.3:-1434244	MS.gene032599:CDS
ATCAATGACACCAAGACCGG+TGG	0.659585	6.3:-1434570	MS.gene032599:intron
ATTATCCACTTGAAAAGCTT+TGG	0.360291	6.3:+1433898	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTATTGCTAATGCTGTATT+TGG	-	chr6.3:1434563-1434582	MS.gene032599:intron	30.0%
AGCAAATGATTTCTTCTACT+CGG	-	chr6.3:1434187-1434206	MS.gene032599:CDS	30.0%
ATTATCCACTTGAAAAGCTT+TGG	+	chr6.3:1434621-1434640	None:intergenic	30.0%
ATTCACTTCCAATTGAATGT+GGG	-	chr6.3:1434485-1434504	MS.gene032599:intron	30.0%
CTATGATACAAAAGTTTCAC+GGG	-	chr6.3:1434040-1434059	MS.gene032599:CDS	30.0%
CTATTGCTAATGCTGTATTT+GGG	-	chr6.3:1434564-1434583	MS.gene032599:intron	30.0%
GTTACTGTTAATGAGATACT+AGG	-	chr6.3:1434257-1434276	MS.gene032599:CDS	30.0%
TACTGAGATTCTTATAGTGT+TGG	-	chr6.3:1434361-1434380	MS.gene032599:CDS	30.0%
TTAGCAATAGTAATAACACC+TGG	+	chr6.3:1434554-1434573	None:intergenic	30.0%
ATAAGAATCTCAGTAGCTCT+AGG	+	chr6.3:1434356-1434375	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	1433850	1434688	1433850	ID=MS.gene032599
chr6.3	mRNA	1433850	1434688	1433850	ID=MS.gene032599.t1;Parent=MS.gene032599
chr6.3	exon	1434571	1434688	1434571	ID=MS.gene032599.t1.exon1;Parent=MS.gene032599.t1
chr6.3	CDS	1434571	1434688	1434571	ID=cds.MS.gene032599.t1;Parent=MS.gene032599.t1
chr6.3	exon	1433850	1434394	1433850	ID=MS.gene032599.t1.exon2;Parent=MS.gene032599.t1
chr6.3	CDS	1433850	1434394	1433850	ID=cds.MS.gene032599.t1;Parent=MS.gene032599.t1

Gene Sequence

>MS.gene032599

ATGAAGAGTATTTACTTGTTTTTCACAGCCTTCTTAGCATTGGCATCTTGTGCCTTTGCATTTGATCCTAGCCCTCTACAAGACTTTTGTGTGGCAATCAATGACACCAAGACCGGTGGTATGTATACTTTTTAATACTTCTTCATTTATTTATTTTGACTCGTTTGGTCAACATGTTGAACCAATTGAACTATGATACAAAAGTTTCACGGGTGATTTCATGTCTAACAACGCTGATATATACATATATAAGTAGAGCATGTTATTTAATTACATGATGAATTTGTTTTGCAGTTTTTGTGAATGGCAAATTCTGCAAGGATCCAAAACTTGCTACAGCAAATGATTTCTTCTACTCGGTGAAAGAAGGAAATACATCAAATCCACTTGGTTCAAAAGTGACATCAGTTACTGTTAATGAGATACTAGGACTAAACACTCTAGGTATATCTCTTGCTCGTATTGACTTCGCTGCTAGGGGACTAAATCCTCCACATACTCACCCTAGAGCTACTGAGATTCTTATAGTGTTGGAGGGTACTCTTTATGTTGGGTTTGTTACTTCTAATCCAGAAAACCGTCTCATCACAAAAATACTCAACAAAGGAGATGTTTTTGTGTTTCCAATTGGTCTCATTCACTTCCAATTGAATGTGGGGTATGGAAATGCGGTTGCCATTGCTGGACTTAGCAGCCAAAATCCAGGTGTTATTACTATTGCTAATGCTGTATTTGGGTCTAATCCAAAGATCTCTTCAGAAGTTCTCACCAAAGCTTTTCAAGTGGATAATAAAATAGTGAACAATCTTCAAAAACAATTTTGGGTGGACAACAACTAG

Protein sequence

>MS.gene032599.t1

MKSIYLFFTAFLALASCAFAFDPSPLQDFCVAINDTKTGVFVNGKFCKDPKLATANDFFYSVKEGNTSNPLGSKVTSVTVNEILGLNTLGISLARIDFAARGLNPPHTHPRATEILIVLEGTLYVGFVTSNPENRLITKILNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANAVFGSNPKISSEVLTKAFQVDNKIVNNLQKQFWVDNN