AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037517.t1 MTR_5g046410 98.361 183 2 1 22 204 40 221 2.60e-129 363
MS.gene037517.t1 MTR_5g046430 98.361 183 2 1 22 204 40 221 2.60e-129 363
MS.gene037517.t1 MTR_4g017030 98.361 183 2 1 22 204 40 221 2.60e-129 363
MS.gene037517.t1 MTR_4g017050 97.814 183 3 1 22 204 40 221 9.37e-129 361
MS.gene037517.t1 MTR_4g017040 97.268 183 4 1 22 204 40 221 6.46e-128 359
MS.gene037517.t1 MTR_2g019250 96.175 183 6 1 22 204 40 221 3.16e-127 357
MS.gene037517.t1 MTR_1g079490 80.978 184 33 2 22 204 40 222 1.99e-104 300
MS.gene037517.t1 MTR_6g005340 78.142 183 38 1 22 204 40 220 1.60e-99 287
MS.gene037517.t1 MTR_6g005310 78.022 182 38 1 22 203 40 219 7.34e-99 286
MS.gene037517.t1 MTR_6g005330 77.596 183 39 1 22 204 40 220 7.58e-99 286
MS.gene037517.t1 MTR_6g005360 77.596 183 39 1 22 204 40 220 9.86e-99 285
MS.gene037517.t1 MTR_6g005350 67.213 183 57 2 22 204 40 219 5.60e-78 233
MS.gene037517.t1 MTR_2g030855 64.571 175 56 3 27 198 40 211 1.37e-73 221
MS.gene037517.t1 MTR_1g023770 65.896 173 53 3 29 198 39 208 5.14e-73 220
MS.gene037517.t1 MTR_6g005380 62.941 170 62 1 22 191 40 208 9.27e-73 219
MS.gene037517.t1 MTR_2g030895 64.368 174 55 4 29 198 7 177 2.34e-71 214
MS.gene037517.t1 MTR_2g031270 64.000 175 57 3 27 198 41 212 3.88e-71 215
MS.gene037517.t1 MTR_2g030865 63.793 174 56 4 29 198 7 177 1.68e-69 210
MS.gene037517.t1 MTR_2g031300 54.651 172 52 4 29 197 7 155 9.53e-52 164
MS.gene037517.t1 MTR_1g085500 46.927 179 89 2 22 198 39 213 2.30e-51 165
MS.gene037517.t1 MTR_1g085140 46.369 179 90 2 22 198 40 214 1.02e-47 155
MS.gene037517.t1 MTR_7g111240 46.927 179 89 2 22 198 36 210 1.14e-47 155
MS.gene037517.t1 MTR_7g111240 47.458 177 87 2 24 198 35 207 3.17e-47 154
MS.gene037517.t1 MTR_5g036570 44.693 179 92 3 23 198 44 218 7.67e-42 141
MS.gene037517.t1 MTR_1g112200 43.860 171 89 3 29 198 42 206 9.47e-40 135
MS.gene037517.t1 MTR_2g041550 44.509 173 88 3 26 196 37 203 4.25e-39 133
MS.gene037517.t1 MTR_2g041570 40.230 174 96 3 26 197 37 204 2.44e-35 124
MS.gene037517.t1 MTR_2g041580 40.462 173 95 3 26 196 37 203 3.52e-35 123
MS.gene037517.t1 MTR_1g060120 38.596 171 98 3 29 198 56 220 8.40e-35 123
MS.gene037517.t1 MTR_8g020750 44.595 148 75 2 25 170 39 181 2.02e-33 119
MS.gene037517.t1 MTR_8g007300 40.437 183 95 4 22 202 44 214 6.30e-33 118
MS.gene037517.t1 MTR_8g020590 38.286 175 100 3 25 197 34 202 2.51e-30 111
MS.gene037517.t1 MTR_3g019710 42.038 157 84 3 25 180 37 187 3.02e-30 110
MS.gene037517.t1 MTR_8g020630 41.727 139 75 2 25 162 37 170 1.99e-29 108
MS.gene037517.t1 MTR_8g020650 36.000 175 104 3 25 198 37 204 4.53e-29 107
MS.gene037517.t1 MTR_2g086620 42.424 132 69 3 67 197 77 202 2.18e-28 105
MS.gene037517.t1 MTR_2g086630 41.667 132 70 3 67 197 77 202 7.06e-28 104
MS.gene037517.t1 MTR_8g020770 43.478 138 70 2 25 159 38 170 9.06e-28 104
MS.gene037517.t1 MTR_2g086640 41.667 132 70 3 67 197 76 201 1.00e-27 104
MS.gene037517.t1 MTR_3g019650 40.127 157 87 3 25 180 36 186 1.17e-27 104
MS.gene037517.t1 MTR_2g036120 42.336 137 73 2 25 160 37 168 1.44e-27 103
MS.gene037517.t1 MTR_2g045100 41.606 137 74 2 25 160 37 168 1.84e-27 103
MS.gene037517.t1 MTR_4g052770 43.182 132 68 3 67 197 78 203 2.17e-27 103
MS.gene037517.t1 MTR_4g052780 43.511 131 67 3 68 197 79 203 2.24e-27 103
MS.gene037517.t1 MTR_3g019680 40.127 157 87 3 25 180 36 186 2.47e-27 103
MS.gene037517.t1 MTR_8g020670 34.857 175 106 3 25 198 37 204 2.02e-26 100
MS.gene037517.t1 MTR_2g044040 39.416 137 77 2 25 160 37 168 1.21e-25 99.0
MS.gene037517.t1 MTR_4g010340 37.143 175 101 4 25 198 34 200 1.93e-25 98.2
MS.gene037517.t1 MTR_3g096330 42.254 142 75 3 23 162 596 732 4.77e-25 102
MS.gene037517.t1 MTR_0284s0010 40.288 139 77 2 25 162 37 170 1.34e-24 96.3
MS.gene037517.t1 MTR_8g020763 35.227 176 105 3 25 198 17 185 1.86e-24 95.5
MS.gene037517.t1 MTR_5g031460 38.571 140 80 2 24 162 36 170 1.06e-23 94.0
MS.gene037517.t1 MTR_7g011340 37.278 169 97 3 25 189 37 200 2.75e-23 92.8
MS.gene037517.t1 MTR_8g020760 36.571 175 103 3 25 198 39 206 4.76e-23 92.4
MS.gene037517.t1 MTR_3g073170 34.857 175 103 4 25 198 41 205 5.23e-23 92.0
MS.gene037517.t1 MTR_2g019780 33.714 175 105 4 25 198 43 207 2.16e-22 90.5
MS.gene037517.t1 MTR_8g020610 34.483 174 104 5 25 196 35 200 1.83e-21 88.2
MS.gene037517.t1 MTR_2g030915 49.351 77 23 1 75 151 1 61 2.31e-18 77.0
MS.gene037517.t1 MTR_4g027415 38.182 110 62 2 26 134 38 142 8.81e-15 68.9
MS.gene037517.t1 MTR_3g019810 32.515 163 95 6 25 185 143 292 1.47e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037517.t1 AT3G05950 66.310 187 58 3 22 204 43 228 4.67e-81 241
MS.gene037517.t1 AT5G39160 64.804 179 60 2 22 198 41 218 3.29e-77 231
MS.gene037517.t1 AT5G39190 64.804 179 60 2 22 198 41 218 5.04e-77 231
MS.gene037517.t1 AT5G39130 63.687 179 62 2 22 198 41 218 1.66e-76 229
MS.gene037517.t1 AT5G39110 63.687 179 62 2 22 198 42 219 5.63e-76 228
MS.gene037517.t1 AT5G39150 63.687 179 62 2 22 198 41 218 7.49e-76 228
MS.gene037517.t1 AT5G39120 63.687 179 62 2 22 198 41 218 9.84e-76 227
MS.gene037517.t1 AT5G39180 63.687 179 62 2 22 198 41 218 1.35e-75 227
MS.gene037517.t1 AT5G39160 64.000 175 60 2 26 198 41 214 6.05e-74 223
MS.gene037517.t1 AT4G14630 62.570 179 64 2 22 198 44 221 5.08e-71 215
MS.gene037517.t1 AT3G04200 63.277 177 62 3 22 196 44 219 9.42e-71 215
MS.gene037517.t1 AT5G38940 61.453 179 65 2 22 198 44 220 4.17e-70 213
MS.gene037517.t1 AT5G38930 62.570 179 63 2 22 198 44 220 5.54e-70 213
MS.gene037517.t1 AT5G38940 61.453 179 65 2 22 198 68 244 5.82e-70 213
MS.gene037517.t1 AT5G39160 56.771 192 65 4 15 198 15 196 3.33e-68 207
MS.gene037517.t1 AT5G39190 56.771 192 65 4 15 198 15 196 4.06e-68 207
MS.gene037517.t1 AT5G38960 60.556 180 66 3 22 198 44 221 6.02e-67 205
MS.gene037517.t1 AT5G38910 61.111 180 64 3 22 198 42 218 1.29e-65 201
MS.gene037517.t1 AT3G04150 53.093 194 84 3 10 197 40 232 7.80e-63 195
MS.gene037517.t1 AT3G04150 55.866 179 76 2 21 197 42 219 1.85e-62 194
MS.gene037517.t1 AT3G04190 55.932 177 75 2 22 196 43 218 3.84e-62 192
MS.gene037517.t1 AT3G04170 54.494 178 75 3 22 197 43 216 1.81e-59 186
MS.gene037517.t1 AT3G04180 53.672 177 79 2 22 196 43 218 1.68e-58 183
MS.gene037517.t1 AT1G02335 46.328 177 89 3 24 198 42 214 5.59e-49 159
MS.gene037517.t1 AT1G09560 46.328 177 89 2 24 198 42 214 1.45e-48 158
MS.gene037517.t1 AT5G26700 50.000 170 72 4 22 187 37 197 4.29e-48 157
MS.gene037517.t1 AT3G62020 45.902 183 92 3 19 198 7 185 5.56e-48 155
MS.gene037517.t1 AT3G05930 49.697 165 76 2 24 185 41 201 1.16e-47 155
MS.gene037517.t1 AT3G62020 47.191 178 87 3 24 198 41 214 1.18e-46 153
MS.gene037517.t1 AT1G18970 45.198 177 89 3 24 198 47 217 5.23e-44 146
MS.gene037517.t1 AT1G18980 45.763 177 88 3 24 198 47 217 5.13e-43 144
MS.gene037517.t1 AT5G38910 47.222 180 52 3 22 198 42 181 2.12e-41 139
MS.gene037517.t1 AT1G10460 41.436 181 98 4 22 198 38 214 2.23e-40 137
MS.gene037517.t1 AT3G10080 40.351 171 95 3 29 198 54 218 3.95e-38 131
MS.gene037517.t1 AT5G61750 46.309 149 74 2 23 169 38 182 5.93e-38 130
MS.gene037517.t1 AT3G10080 40.351 171 95 3 29 198 125 289 1.88e-37 132
MS.gene037517.t1 AT5G20630 41.420 169 92 4 25 191 37 200 1.39e-33 119
MS.gene037517.t1 AT1G72610 39.884 173 96 5 26 196 35 201 2.44e-30 111
MS.gene037517.t1 AT1G74820 38.068 176 101 3 25 198 55 224 3.89e-29 108
MS.gene037517.t1 AT5G38950 54.167 72 33 0 128 199 2 73 6.56e-19 78.6

Find 43 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAACTGAATGTTGGATA+TGG 0.274546 4.3:+82528401 MS.gene037517:CDS
TAACACTCTTGGTATATCTT+TGG 0.286436 4.3:+82528187 MS.gene037517:CDS
ATGGTCTTTCAGTTTATTAA+TGG 0.311945 4.3:+82527100 MS.gene037517:CDS
TTGAGAGCCTAGTGCGTTAG+AGG 0.314204 4.3:-82528129 None:intergenic
CTTATCCACTTGAAAAGCTT+TGG 0.335788 4.3:-82528528 None:intergenic
TGAAAATGAATAAGACCAAT+TGG 0.356438 4.3:-82528383 None:intergenic
GAGTGCCTTCAAGTACTATA+AGG 0.386700 4.3:-82528274 None:intergenic
CTCTAGGGTGGATGTGAGGT+GGG 0.411548 4.3:-82528241 None:intergenic
TTCTTCAAACATATTGAAGC+TGG 0.411728 4.3:+82528101 MS.gene037517:CDS
GATGTGTTTGTGTTCCCAAT+TGG 0.424230 4.3:+82528368 MS.gene037517:CDS
TTGAAAGGGTACATCGGAAA+TGG 0.427539 4.3:+82527081 MS.gene037517:intron
GGCAACGCGGTTGCTATCGC+TGG 0.429748 4.3:+82528422 MS.gene037517:CDS
ATTATCTTCAAAAGCAGTCT+TGG 0.442184 4.3:+82528561 MS.gene037517:CDS
TCTTCAGCTTTAACAAGTGC+GGG 0.444792 4.3:-82528077 None:intergenic
GTCACTGTAGACCAACTATT+CGG 0.446134 4.3:+82528161 MS.gene037517:CDS
CTTGAAGGCACTCTTTATGT+TGG 0.454430 4.3:+82528284 MS.gene037517:CDS
TACTTTGCAGTATTTGTGAA+TGG 0.463825 4.3:+82528038 MS.gene037517:intron
ATAAGGATCTCTGTTCCTCT+AGG 0.468428 4.3:-82528257 None:intergenic
GAGATCCTTATAGTACTTGA+AGG 0.469573 4.3:+82528269 MS.gene037517:CDS
TTTGCAACGGTGATAACTCC+TGG 0.470922 4.3:-82528461 None:intergenic
CTATTCGGACTTAACACTCT+TGG 0.480493 4.3:+82528176 MS.gene037517:CDS
CCTCTAGGGTGGATGTGAGG+TGG 0.496940 4.3:-82528242 None:intergenic
GAAAATGAATAAGACCAATT+GGG 0.510497 4.3:-82528382 None:intergenic
AGAACTTCGTCGGAAATAAG+AGG 0.511108 4.3:-82528503 None:intergenic
TCTTCAAACATATTGAAGCT+GGG 0.520485 4.3:+82528102 MS.gene037517:CDS
GAGTGTTAAGTCCGAATAGT+TGG 0.535207 4.3:-82528172 None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG 0.537084 4.3:-82528227 None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG 0.543612 4.3:-82528078 None:intergenic
CTTTACCAAGGTGCTCAACA+AGG 0.544491 4.3:+82528343 MS.gene037517:CDS
AGCTTTGGTAAGAACTTCGT+CGG 0.555884 4.3:-82528513 None:intergenic
ATCACCCTTGTTGAGCACCT+TGG 0.567836 4.3:-82528348 None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG 0.571738 4.3:-82528256 None:intergenic
GGGAATGCCTCTAACGCACT+AGG 0.573570 4.3:+82528122 MS.gene037517:CDS
GGACTTAGCAGTCAAAATCC+AGG 0.578792 4.3:+82528443 MS.gene037517:CDS
GGATCTCTGTTCCTCTAGGG+TGG 0.590427 4.3:-82528253 None:intergenic
TTTAAACAAAGCATTTGCAA+CGG 0.590900 4.3:-82528474 None:intergenic
TATTTGTTGAAAGGGTACAT+CGG 0.640033 4.3:+82527075 MS.gene037517:intron
GTTCCTCTAGGGTGGATGTG+AGG 0.650118 4.3:-82528245 None:intergenic
TTTACCAAGGTGCTCAACAA+GGG 0.659466 4.3:+82528344 MS.gene037517:CDS
CCACCTCACATCCACCCTAG+AGG 0.660499 4.3:+82528242 MS.gene037517:CDS
CTTCAGCTTTAACAAGTGCG+GGG 0.662073 4.3:-82528076 None:intergenic
CAATAATCGTCTCTTTACCA+AGG 0.672342 4.3:+82528331 MS.gene037517:CDS
GAATGTTGGATATGGCAACG+CGG 0.840401 4.3:+82528409 MS.gene037517:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTATAATTTTATAA+TGG - chr4.3:82526993-82527012 None:intergenic 0.0%
!!! AAAAAAAAAAATTTTTTGCA+GGG - chr4.3:82527905-82527924 None:intergenic 10.0%
!!! ATTTTATAATGAAATTGTTT+TGG - chr4.3:82527413-82527432 None:intergenic 10.0%
!!! TAAAAAAAAAAATTTTTTGC+AGG - chr4.3:82527906-82527925 None:intergenic 10.0%
!!! TTTTATAATGAAATTGTTTT+GGG - chr4.3:82527412-82527431 None:intergenic 10.0%
!! AATAATTATGTATTAACTAG+AGG - chr4.3:82527059-82527078 None:intergenic 15.0%
!! AGATAATTGAAATTCATTTA+AGG + chr4.3:82527247-82527266 MS.gene037517:intron 15.0%
!! ATAATTATGTATTAACTAGA+GGG - chr4.3:82527058-82527077 None:intergenic 15.0%
!! GATAATTGAAATTCATTTAA+GGG + chr4.3:82527248-82527267 MS.gene037517:intron 15.0%
!!! AAATACTTTTATCTTTATGA+AGG + chr4.3:82526585-82526604 MS.gene037517:intron 15.0%
!!! AAATATAGAATCTTTTGTTT+TGG + chr4.3:82527939-82527958 MS.gene037517:intron 15.0%
!!! AATACTTTTATCTTTATGAA+GGG + chr4.3:82526586-82526605 MS.gene037517:intron 15.0%
!!! ATAAAAGTATTTCATTTCAT+AGG - chr4.3:82526577-82526596 None:intergenic 15.0%
!!! ATACATAATTATTTGTTGAA+AGG + chr4.3:82527066-82527085 MS.gene037517:intron 15.0%
!!! TACATAATTATTTGTTGAAA+GGG + chr4.3:82527067-82527086 MS.gene037517:intron 15.0%
!!! TTTGCAATTTTAATTAGTAT+TGG - chr4.3:82526113-82526132 None:intergenic 15.0%
!! AAGAAGAAGAAGATAAATTT+AGG - chr4.3:82527184-82527203 None:intergenic 20.0%
!! AATTGCAAATATAGTACTTT+AGG + chr4.3:82526124-82526143 MS.gene037517:intron 20.0%
!! GATCAAAATGAAAAAACAAA+CGG + chr4.3:82526042-82526061 MS.gene037517:CDS 20.0%
!!! ATCTTCTTCTTCTTTTTAAA+CGG + chr4.3:82527190-82527209 MS.gene037517:intron 20.0%
!!! ATTTTATAATGGAAAAACTC+TGG - chr4.3:82526982-82527001 None:intergenic 20.0%
!!! TCTGTTTTTATTTGAACTTT+TGG + chr4.3:82526694-82526713 MS.gene037517:intron 20.0%
!!! TTTTATAATGGAAAAACTCT+GGG - chr4.3:82526981-82527000 None:intergenic 20.0%
! AAGTACAATCTACATGAAAA+AGG - chr4.3:82526435-82526454 None:intergenic 25.0%
! AGTACAATCTACATGAAAAA+GGG - chr4.3:82526434-82526453 None:intergenic 25.0%
! ATGGTCTTTCAGTTTATTAA+TGG + chr4.3:82527100-82527119 MS.gene037517:CDS 25.0%
! CGTATAGCAAAAATATATGT+TGG - chr4.3:82527285-82527304 None:intergenic 25.0%
! GAAAATGAATAAGACCAATT+GGG - chr4.3:82528385-82528404 None:intergenic 25.0%
! GTAATCAAGACTTAAAGTTA+AGG - chr4.3:82527821-82527840 None:intergenic 25.0%
! GTACTAAACTGATCTTAATT+TGG + chr4.3:82527019-82527038 MS.gene037517:intron 25.0%
! TACATGTAGTTGTTAAAGAA+TGG + chr4.3:82527509-82527528 MS.gene037517:intron 25.0%
! TGAAAATGAATAAGACCAAT+TGG - chr4.3:82528386-82528405 None:intergenic 25.0%
! TTGGAAAATATTCGAGTAAT+TGG - chr4.3:82527136-82527155 None:intergenic 25.0%
!! ATTCATTTTCAACTGAATGT+TGG + chr4.3:82528395-82528414 MS.gene037517:CDS 25.0%
!! TAGGATTTTTCTTAGTAAGT+TGG - chr4.3:82527165-82527184 None:intergenic 25.0%
!! TCATTTAACCATTTTATCCA+GGG - chr4.3:82527328-82527347 None:intergenic 25.0%
!! TTTAAACAAAGCATTTGCAA+CGG - chr4.3:82528477-82528496 None:intergenic 25.0%
AAAATTACCATTACGAGAAC+AGG + chr4.3:82527428-82527447 MS.gene037517:intron 30.0%
AATAACATGCAGTCATCAAA+AGG + chr4.3:82526875-82526894 MS.gene037517:intron 30.0%
AGAAACATGTCTTGCTAATT+AGG - chr4.3:82526677-82526696 None:intergenic 30.0%
ATATACATACCACCATCTTT+GGG - chr4.3:82527795-82527814 None:intergenic 30.0%
ATCACTAATACATGTCACAA+AGG - chr4.3:82526776-82526795 None:intergenic 30.0%
ATCTACATGAAAAAGGGATA+TGG - chr4.3:82526428-82526447 None:intergenic 30.0%
ATTATCTTCAAAAGCAGTCT+TGG + chr4.3:82528561-82528580 MS.gene037517:CDS 30.0%
CCAAAAAGTCTAATATTTGC+AGG - chr4.3:82527875-82527894 None:intergenic 30.0%
GAAGTTGGCAATTGAAATTA+AGG + chr4.3:82526529-82526548 MS.gene037517:intron 30.0%
TAACACTCTTGGTATATCTT+TGG + chr4.3:82528187-82528206 MS.gene037517:CDS 30.0%
TACTTTGCAGTATTTGTGAA+TGG + chr4.3:82528038-82528057 MS.gene037517:intron 30.0%
TAGTAGTTGTGGAATTAATG+TGG - chr4.3:82526832-82526851 None:intergenic 30.0%
TATATACATACCACCATCTT+TGG - chr4.3:82527796-82527815 None:intergenic 30.0%
TCTTCAAACATATTGAAGCT+GGG + chr4.3:82528102-82528121 MS.gene037517:CDS 30.0%
TGACTAGGTTAATTCAGAAA+CGG + chr4.3:82526737-82526756 MS.gene037517:intron 30.0%
TGGCAATTTCTTGAGTAATT+TGG - chr4.3:82526812-82526831 None:intergenic 30.0%
TGGGATTTATGTCTTATCAT+GGG - chr4.3:82526161-82526180 None:intergenic 30.0%
TTCTTCAAACATATTGAAGC+TGG + chr4.3:82528101-82528120 MS.gene037517:CDS 30.0%
TTGGGATTTATGTCTTATCA+TGG - chr4.3:82526162-82526181 None:intergenic 30.0%
TTTCAACTGAATGTTGGATA+TGG + chr4.3:82528401-82528420 MS.gene037517:CDS 30.0%
TTTGTGACATGTATTAGTGA+TGG + chr4.3:82526775-82526794 MS.gene037517:intron 30.0%
! AATATTTGACTTATGCGTTG+CGG - chr4.3:82527218-82527237 None:intergenic 30.0%
! CCTGCAAATATTAGACTTTT+TGG + chr4.3:82527872-82527891 MS.gene037517:intron 30.0%
! GTCATTTAACCATTTTATCC+AGG - chr4.3:82527329-82527348 None:intergenic 30.0%
! TATTTGAACTTTTGGATCCT+CGG + chr4.3:82526702-82526721 MS.gene037517:intron 30.0%
! TATTTGTTGAAAGGGTACAT+CGG + chr4.3:82527075-82527094 MS.gene037517:intron 30.0%
!! GATTTTTCTTAGTAAGTTGG+TGG - chr4.3:82527162-82527181 None:intergenic 30.0%
!!! GAATCTTTTGTTTTGGTTCT+TGG + chr4.3:82527946-82527965 MS.gene037517:intron 30.0%
AAGATAAGTGTAGACCAACA+CGG - chr4.3:82527377-82527396 None:intergenic 35.0%
AAGTTGACCTTGCTTGATAA+AGG - chr4.3:82526337-82526356 None:intergenic 35.0%
AGTCAACAAGTAATTAACCG+AGG - chr4.3:82526722-82526741 None:intergenic 35.0%
AGTTGACCTTGCTTGATAAA+GGG - chr4.3:82526336-82526355 None:intergenic 35.0%
ATACTTCCTTGTTAGCATCT+TGG + chr4.3:82527697-82527716 MS.gene037517:intron 35.0%
ATCAAACTCCCTGGATAAAA+TGG + chr4.3:82527317-82527336 MS.gene037517:intron 35.0%
CAATAATCGTCTCTTTACCA+AGG + chr4.3:82528331-82528350 MS.gene037517:CDS 35.0%
CTTATCCACTTGAAAAGCTT+TGG - chr4.3:82528531-82528550 None:intergenic 35.0%
GACTGCATTCGTAAAAATTG+CGG + chr4.3:82526085-82526104 MS.gene037517:intron 35.0%
GAGATCCTTATAGTACTTGA+AGG + chr4.3:82528269-82528288 MS.gene037517:CDS 35.0%
GGCACAAGTGATATATGATT+CGG - chr4.3:82527351-82527370 None:intergenic 35.0%
TATACATACCACCATCTTTG+GGG - chr4.3:82527794-82527813 None:intergenic 35.0%
TATCAAGCAAGGTCAACTTA+AGG + chr4.3:82526338-82526357 MS.gene037517:intron 35.0%
TGTACAAAGTGTAAGATCAC+AGG - chr4.3:82526279-82526298 None:intergenic 35.0%
TTATCTACCTGTTCTCGTAA+TGG - chr4.3:82527438-82527457 None:intergenic 35.0%
TTTAGACTTTGTCTCAGTTC+TGG - chr4.3:82526938-82526957 None:intergenic 35.0%
! AGTACTGAGTTGCAAAATGA+AGG + chr4.3:82527670-82527689 MS.gene037517:intron 35.0%
! ATCTTCAGCTTTAACAAGTG+CGG - chr4.3:82528081-82528100 None:intergenic 35.0%
! CGGTTAATTACTTGTTGACT+AGG + chr4.3:82526722-82526741 MS.gene037517:intron 35.0%
!! CTTAGTAAGTTGGTGGAATT+TGG - chr4.3:82527155-82527174 None:intergenic 35.0%
!! GTTCTTGGAACATTAGCTTT+GGG - chr4.3:82526257-82526276 None:intergenic 35.0%
!! TCTTACCAAAGCTTTTCAAG+TGG + chr4.3:82528523-82528542 MS.gene037517:CDS 35.0%
AGAACTTCGTCGGAAATAAG+AGG - chr4.3:82528506-82528525 None:intergenic 40.0%
AGTAATGACCTACCTAAACC+AGG - chr4.3:82527977-82527996 None:intergenic 40.0%
AGTGTAAGATCACAGGTTCT+TGG - chr4.3:82526272-82526291 None:intergenic 40.0%
ATAAGGATCTCTGTTCCTCT+AGG - chr4.3:82528260-82528279 None:intergenic 40.0%
ATGTCTTATCATGGGACTAC+TGG - chr4.3:82526153-82526172 None:intergenic 40.0%
CAAACATCGTGTAGTAGTTG+TGG - chr4.3:82526843-82526862 None:intergenic 40.0%
CTATTCGGACTTAACACTCT+TGG + chr4.3:82528176-82528195 MS.gene037517:CDS 40.0%
GAAAAAGGGATATGGCTGAA+AGG - chr4.3:82526420-82526439 None:intergenic 40.0%
GAGTGCCTTCAAGTACTATA+AGG - chr4.3:82528277-82528296 None:intergenic 40.0%
GAGTGTTAAGTCCGAATAGT+TGG - chr4.3:82528175-82528194 None:intergenic 40.0%
GATCCTGAAGGAGGAATATT+TGG - chr4.3:82526475-82526494 None:intergenic 40.0%
GATGTGTTTGTGTTCCCAAT+TGG + chr4.3:82528368-82528387 MS.gene037517:CDS 40.0%
GTCACTGTAGACCAACTATT+CGG + chr4.3:82528161-82528180 MS.gene037517:CDS 40.0%
TAAGGATCTCTGTTCCTCTA+GGG - chr4.3:82528259-82528278 None:intergenic 40.0%
TATCCAGGGAGTTTGATCTT+CGG - chr4.3:82527314-82527333 None:intergenic 40.0%
TGATTGCAACGCAAAAGTCT+TGG - chr4.3:82527767-82527786 None:intergenic 40.0%
TTCAATGTCAAGTGAACACG+TGG + chr4.3:82527459-82527478 MS.gene037517:intron 40.0%
! AACAATCTAGAGCTCAGGTA+TGG - chr4.3:82526186-82526205 None:intergenic 40.0%
! ACAATCTAGAGCTCAGGTAT+GGG - chr4.3:82526185-82526204 None:intergenic 40.0%
! AGCTTTGGTAAGAACTTCGT+CGG - chr4.3:82528516-82528535 None:intergenic 40.0%
! GGTTCTTGGAACATTAGCTT+TGG - chr4.3:82526258-82526277 None:intergenic 40.0%
! GTTGACACACTTGTTGAAGT+TGG + chr4.3:82526514-82526533 MS.gene037517:intron 40.0%
! TCTTCAGCTTTAACAAGTGC+GGG - chr4.3:82528080-82528099 None:intergenic 40.0%
! TTGAAAGGGTACATCGGAAA+TGG + chr4.3:82527081-82527100 MS.gene037517:intron 40.0%
! TTTACCAAGGTGCTCAACAA+GGG + chr4.3:82528344-82528363 MS.gene037517:CDS 40.0%
!! ACGTGGTCTTGACTTTGTTT+TGG + chr4.3:82527476-82527495 MS.gene037517:intron 40.0%
!! CTTGAAGGCACTCTTTATGT+TGG + chr4.3:82528284-82528303 MS.gene037517:CDS 40.0%
!! TTCTTGGTGTTTCCTGGTTT+AGG + chr4.3:82527962-82527981 MS.gene037517:intron 40.0%
!!! TTTTGGTTCTTGGTGTTTCC+TGG + chr4.3:82527956-82527975 MS.gene037517:intron 40.0%
ACAGCACCCTTTATCAAGCA+AGG + chr4.3:82526327-82526346 MS.gene037517:intron 45.0%
ATCAAAGACCCCAAAGATGG+TGG + chr4.3:82527783-82527802 MS.gene037517:intron 45.0%
CAACGCAAAAGTCTTGGAGA+GGG - chr4.3:82527761-82527780 None:intergenic 45.0%
CAGCCAAATATTCCTCCTTC+AGG + chr4.3:82526469-82526488 MS.gene037517:intron 45.0%
CCAAAGCCAAGATGCTAACA+AGG - chr4.3:82527706-82527725 None:intergenic 45.0%
CCTTGTTAGCATCTTGGCTT+TGG + chr4.3:82527703-82527722 MS.gene037517:intron 45.0%
GAATGTTGGATATGGCAACG+CGG + chr4.3:82528409-82528428 MS.gene037517:CDS 45.0%
GCAATCAAAGACCCCAAAGA+TGG + chr4.3:82527780-82527799 MS.gene037517:intron 45.0%
GCGCGAAATTACAAGTGCTA+CGG - chr4.3:82526368-82526387 None:intergenic 45.0%
GGACTTAGCAGTCAAAATCC+AGG + chr4.3:82528443-82528462 MS.gene037517:CDS 45.0%
TAACCGAAGATCAAACTCCC+TGG + chr4.3:82527308-82527327 MS.gene037517:intron 45.0%
TATTGCCATGACGATCCTGA+AGG - chr4.3:82526487-82526506 None:intergenic 45.0%
TGGTGTTTCCTGGTTTAGGT+AGG + chr4.3:82527966-82527985 MS.gene037517:intron 45.0%
TTTGCAACGGTGATAACTCC+TGG - chr4.3:82528464-82528483 None:intergenic 45.0%
! CTTCAGCTTTAACAAGTGCG+GGG - chr4.3:82528079-82528098 None:intergenic 45.0%
! CTTTACCAAGGTGCTCAACA+AGG + chr4.3:82528343-82528362 MS.gene037517:CDS 45.0%
! GCAACAACAATCTAGAGCTC+AGG - chr4.3:82526191-82526210 None:intergenic 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG - chr4.3:82528230-82528249 None:intergenic 45.0%
!! ATTTAATAAAAAATTAATGA+CGG + chr4.3:82527538-82527557 MS.gene037517:intron 5.0%
AAAAGTCTTGGAGAGGGCTG+GGG - chr4.3:82527755-82527774 None:intergenic 50.0%
AAGTGTAGACCAACACGGTG+TGG - chr4.3:82527372-82527391 None:intergenic 50.0%
ATCACCCTTGTTGAGCACCT+TGG - chr4.3:82528351-82528370 None:intergenic 50.0%
CAAAAGTCTTGGAGAGGGCT+GGG - chr4.3:82527756-82527775 None:intergenic 50.0%
CACTTGTAATTTCGCGCGCA+AGG + chr4.3:82526371-82526390 MS.gene037517:intron 50.0%
CTAGAGCTCAGGTATGGGTT+GGG - chr4.3:82526180-82526199 None:intergenic 50.0%
GCAACGCAAAAGTCTTGGAG+AGG - chr4.3:82527762-82527781 None:intergenic 50.0%
GGGTCATAAGCAAAGGCAAC+AGG - chr4.3:82527734-82527753 None:intergenic 50.0%
TTCCTCCTTCAGGATCGTCA+TGG + chr4.3:82526479-82526498 MS.gene037517:intron 50.0%
TTGAGAGCCTAGTGCGTTAG+AGG - chr4.3:82528132-82528151 None:intergenic 50.0%
! TCTAGAGCTCAGGTATGGGT+TGG - chr4.3:82526181-82526200 None:intergenic 50.0%
!!! CGCGTTGATTTTGCGCCTAA+GGG + chr4.3:82528212-82528231 MS.gene037517:CDS 50.0%
!!! TCGCGTTGATTTTGCGCCTA+AGG + chr4.3:82528211-82528230 MS.gene037517:CDS 50.0%
AAAGTCTTGGAGAGGGCTGG+GGG - chr4.3:82527754-82527773 None:intergenic 55.0%
AGGTACACCACCTTCGTGTG+TGG - chr4.3:82526400-82526419 None:intergenic 55.0%
GCAAAAGTCTTGGAGAGGGC+TGG - chr4.3:82527757-82527776 None:intergenic 55.0%
GGGAATGCCTCTAACGCACT+AGG + chr4.3:82528122-82528141 MS.gene037517:CDS 55.0%
TCACTTGTGCCACACCGTGT+TGG + chr4.3:82527360-82527379 MS.gene037517:intron 55.0%
TGCCATGACGATCCTGAAGG+AGG - chr4.3:82526484-82526503 None:intergenic 55.0%
! AAGGCATCCACACACGAAGG+TGG + chr4.3:82526390-82526409 MS.gene037517:intron 55.0%
! GGATCTCTGTTCCTCTAGGG+TGG - chr4.3:82528256-82528275 None:intergenic 55.0%
!! CTCTAGGGTGGATGTGAGGT+GGG - chr4.3:82528244-82528263 None:intergenic 55.0%
!! GTTCCTCTAGGGTGGATGTG+AGG - chr4.3:82528248-82528267 None:intergenic 55.0%
CCACCTCACATCCACCCTAG+AGG + chr4.3:82528242-82528261 MS.gene037517:CDS 60.0%
GGGCTGGGGGTCATAAGCAA+AGG - chr4.3:82527741-82527760 None:intergenic 60.0%
! CGCAAGGCATCCACACACGA+AGG + chr4.3:82526387-82526406 MS.gene037517:intron 60.0%
!! CCTCTAGGGTGGATGTGAGG+TGG - chr4.3:82528245-82528264 None:intergenic 60.0%
GGCAACGCGGTTGCTATCGC+TGG + chr4.3:82528422-82528441 MS.gene037517:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82526034 82528598 82526034 ID=MS.gene037517
chr4.3 mRNA 82526034 82528598 82526034 ID=MS.gene037517.t1;Parent=MS.gene037517
chr4.3 exon 82526034 82526063 82526034 ID=MS.gene037517.t1.exon1;Parent=MS.gene037517.t1
chr4.3 CDS 82526034 82526063 82526034 ID=cds.MS.gene037517.t1;Parent=MS.gene037517.t1
chr4.3 exon 82527088 82527121 82527088 ID=MS.gene037517.t1.exon2;Parent=MS.gene037517.t1
chr4.3 CDS 82527088 82527121 82527088 ID=cds.MS.gene037517.t1;Parent=MS.gene037517.t1
chr4.3 exon 82528048 82528598 82528048 ID=MS.gene037517.t1.exon3;Parent=MS.gene037517.t1
chr4.3 CDS 82528048 82528598 82528048 ID=cds.MS.gene037517.t1;Parent=MS.gene037517.t1
Gene Sequence

>MS.gene037517

ATGGACACGATCAAAATGAAAAAACAAACGGTAAAAAATTTACATGTTAGTGACTGCATTCGTAAAAATTGCGGTTCCAATACTAATTAAAATTGCAAATATAGTACTTTAGGTCTCCAGTAGTCCCATGATAAGACATAAATCCCAACCCATACCTGAGCTCTAGATTGTTGTTGCATGCTAGAGTTAAATCACAACTTCAAGCGAGCAATGTAATAAACCCAAAGCTAATGTTCCAAGAACCTGTGATCTTACACTTTGTACAACTTGTACAGAAGAGAAAGAGAACTCATACAGCACCCTTTATCAAGCAAGGTCAACTTAAGGATTTCCGTAGCACTTGTAATTTCGCGCGCAAGGCATCCACACACGAAGGTGGTGTACCTTTCAGCCATATCCCTTTTTCATGTAGATTGTACTTGCATGTCTCTATAACAGCCAAATATTCCTCCTTCAGGATCGTCATGGCAATATGAAGTTGTTGACACACTTGTTGAAGTTGGCAATTGAAATTAAGGTTTTACTTCATTTAAATTTTTACCTATGAAATGAAATACTTTTATCTTTATGAAGGGCATTTTAGAGAAATCTACTATATTATTAGAACAAAATATTTAAATAAAAAATTACTACTACTATTCCTAATTAGCAAGACATGTTTCTGTTTTTATTTGAACTTTTGGATCCTCGGTTAATTACTTGTTGACTAGGTTAATTCAGAAACGGTCATTCTATTTTGCCTTTGTGACATGTATTAGTGATGGCATGATTGCGACCAAATTACTCAAGAAATTGCCACATTAATTCCACAACTACTACACGATGTTTGAACTTTAATACTAATAACATGCAGTCATCAAAAGGTTAGTTTGCAAAATAGAGTGCAATTATAATTAAGTCTCCAGAACTGAGACAAAGTCTAAACTGATCTTATTTTGATTTAGCCCAGAGTTTTTCCATTATAAAATTATAATTAAAAGAACATGTACTAAACTGATCTTAATTTGGTTTAAAATCAGAGCCCTCTAGTTAATACATAATTATTTGTTGAAAGGGTACATCGGAAATGGTCTTTCAGTTTATTAATGGTACGCTTGTGCCAATTACTCGAATATTTTCCAAATTCCACCAACTTACTAAGAAAAATCCTAAATTTATCTTCTTCTTCTTTTTAAACGGTACCGCAACGCATAAGTCAAATATTAATCATTTGAGATAATTGAAATTCATTTAAGGGATTGATCTCTACCAACATATATTTTTGCTATACGCGTTAACCGAAGATCAAACTCCCTGGATAAAATGGTTAAATGACCGAATCATATATCACTTGTGCCACACCGTGTTGGTCTACACTTATCTTAATATCATTTAGCCCAAAACAATTTCATTATAAAATTACCATTACGAGAACAGGTAGATAAATTCAATGTCAAGTGAACACGTGGTCTTGACTTTGTTTTGGAGATCAAGTATACATGTAGTTGTTAAAGAATGGTTCATTATTTAATAAAAAATTAATGACGGTAAAGATCAAATGCAAACAACTATAATATTTCATTCTTATAAATAGCAAGCAAATACATGCAACAAAACTCAATTCTAACAAGTTATTTACATAAGAATATTAAAGAGTAGTACTGAGTTGCAAAATGAAGGTTTTATACTTCCTTGTTAGCATCTTGGCTTTGGCATCACCTGTTGCCTTTGCTTATGACCCCCAGCCCTCTCCAAGACTTTTGCGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTAAGTCTTGATTACTAAATTTTTATGCTTAAATATGCAAATGTTTCCTGCAAATATTAGACTTTTTGGTTTAAATCCCTGCAAAAAATTTTTTTTTTTATTTTAGCGCTTGTAAATATAGAATCTTTTGTTTTGGTTCTTGGTGTTTCCTGGTTTAGGTAGGTCATTACTAGACACTATAATTTCTTTGATATAACTAAACTATGATTTGATACTTTGCAGTATTTGTGAATGGAAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATATTGAAGCTGGGAATGCCTCTAACGCACTAGGCTCTCAAGTAACTCAAGTCACTGTAGACCAACTATTCGGACTTAACACTCTTGGTATATCTTTGGCTCGCGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTTGAAGGCACTCTTTATGTTGGATTTGTCACGTCAAATCAAGACAACAATAATCGTCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTTGGATATGGCAACGCGGTTGCTATCGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACCGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG
Protein sequence

>MS.gene037517.t1

MDTIKMKKQTGTSEMVFQFINVFVNGKFCKDPALVKAEDFFKHIEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVTSNQDNNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN