AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70063

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70063.t1 MTR_4g017040 96.970 198 5 1 3 200 25 221 1.26e-137 384
MS.gene70063.t1 MTR_5g046410 96.970 198 5 1 3 200 25 221 1.30e-137 384
MS.gene70063.t1 MTR_5g046430 96.970 198 5 1 3 200 25 221 1.30e-137 384
MS.gene70063.t1 MTR_4g017030 96.970 198 5 1 3 200 25 221 1.30e-137 384
MS.gene70063.t1 MTR_4g017050 96.465 198 6 1 3 200 25 221 4.12e-137 382
MS.gene70063.t1 MTR_2g019250 95.960 198 7 1 3 200 25 221 4.70e-137 382
MS.gene70063.t1 MTR_1g079490 79.899 199 38 2 3 200 25 222 2.23e-110 315
MS.gene70063.t1 MTR_6g005340 78.283 198 41 1 3 200 25 220 3.39e-107 306
MS.gene70063.t1 MTR_6g005310 78.173 197 41 1 3 199 25 219 1.31e-106 305
MS.gene70063.t1 MTR_6g005360 77.778 198 42 1 3 200 25 220 2.28e-106 305
MS.gene70063.t1 MTR_6g005330 77.778 198 42 1 3 200 25 220 2.87e-106 304
MS.gene70063.t1 MTR_6g005350 66.667 198 63 2 3 200 25 219 6.53e-86 253
MS.gene70063.t1 MTR_6g005380 62.567 187 69 1 1 187 23 208 4.13e-78 233
MS.gene70063.t1 MTR_1g023770 60.622 193 64 3 4 194 26 208 1.54e-72 218
MS.gene70063.t1 MTR_2g030855 59.794 194 67 4 4 194 26 211 1.69e-72 218
MS.gene70063.t1 MTR_2g031270 59.278 194 68 4 4 194 27 212 6.57e-70 212
MS.gene70063.t1 MTR_2g030895 63.006 173 57 4 26 194 8 177 4.78e-68 206
MS.gene70063.t1 MTR_2g030865 62.428 173 58 4 26 194 8 177 2.97e-66 201
MS.gene70063.t1 MTR_1g085500 45.596 193 97 3 4 194 27 213 2.37e-52 167
MS.gene70063.t1 MTR_7g111240 46.632 193 95 3 4 194 24 210 3.06e-49 159
MS.gene70063.t1 MTR_2g031300 53.216 171 54 4 26 193 8 155 1.65e-48 155
MS.gene70063.t1 MTR_1g085140 45.078 193 98 3 4 194 28 214 2.47e-48 157
MS.gene70063.t1 MTR_7g111240 46.632 193 92 3 4 194 24 207 5.78e-48 156
MS.gene70063.t1 MTR_5g036570 44.103 195 100 4 3 194 30 218 6.97e-45 148
MS.gene70063.t1 MTR_2g041550 42.857 189 98 4 6 192 23 203 1.60e-38 132
MS.gene70063.t1 MTR_1g112200 39.901 203 89 7 3 194 26 206 2.79e-37 129
MS.gene70063.t1 MTR_1g060120 35.354 198 116 4 3 194 29 220 1.80e-34 122
MS.gene70063.t1 MTR_2g041570 38.421 190 107 4 6 193 23 204 1.40e-33 119
MS.gene70063.t1 MTR_2g041580 38.624 189 106 4 6 192 23 203 1.60e-33 119
MS.gene70063.t1 MTR_8g007300 39.086 197 106 4 4 198 30 214 8.19e-33 117
MS.gene70063.t1 MTR_8g020590 39.583 192 106 5 4 193 19 202 2.63e-32 116
MS.gene70063.t1 MTR_8g020750 38.860 193 100 5 6 192 25 205 1.82e-31 114
MS.gene70063.t1 MTR_3g019710 41.143 175 92 5 4 176 22 187 1.14e-29 109
MS.gene70063.t1 MTR_8g020630 39.286 168 92 4 4 169 22 181 8.96e-29 107
MS.gene70063.t1 MTR_2g086620 42.424 132 69 3 63 193 77 202 9.16e-29 107
MS.gene70063.t1 MTR_8g020650 35.751 193 112 5 4 194 22 204 1.58e-28 106
MS.gene70063.t1 MTR_2g086630 41.667 132 70 3 63 193 77 202 2.64e-28 105
MS.gene70063.t1 MTR_2g086640 41.667 132 70 3 63 193 76 201 3.75e-28 105
MS.gene70063.t1 MTR_4g052780 43.182 132 68 3 63 193 78 203 6.92e-28 104
MS.gene70063.t1 MTR_4g052770 43.182 132 68 3 63 193 78 203 9.14e-28 104
MS.gene70063.t1 MTR_2g036120 38.624 189 99 6 4 185 22 200 2.43e-27 103
MS.gene70063.t1 MTR_3g019650 39.429 175 95 5 4 176 21 186 2.66e-27 103
MS.gene70063.t1 MTR_8g020770 37.895 190 106 4 6 192 25 205 3.70e-27 102
MS.gene70063.t1 MTR_3g019680 39.429 175 95 5 4 176 21 186 5.99e-27 102
MS.gene70063.t1 MTR_8g020670 34.715 193 114 5 4 194 22 204 6.66e-26 99.4
MS.gene70063.t1 MTR_2g044040 37.566 189 101 7 4 185 22 200 9.87e-26 99.0
MS.gene70063.t1 MTR_2g045100 37.566 189 101 6 4 185 22 200 2.12e-25 98.2
MS.gene70063.t1 MTR_0284s0010 39.610 154 83 4 4 155 22 167 1.28e-24 96.3
MS.gene70063.t1 MTR_3g096330 38.854 157 89 3 4 158 581 732 8.64e-24 98.6
MS.gene70063.t1 MTR_5g031460 36.702 188 104 6 4 185 22 200 2.01e-23 93.2
MS.gene70063.t1 MTR_4g010340 37.576 165 94 4 31 194 44 200 6.03e-23 91.7
MS.gene70063.t1 MTR_8g020610 34.921 189 111 6 6 192 22 200 2.26e-22 90.1
MS.gene70063.t1 MTR_7g011340 36.364 187 106 5 4 185 22 200 2.39e-22 90.1
MS.gene70063.t1 MTR_3g073170 34.375 192 110 6 6 194 27 205 1.72e-21 88.2
MS.gene70063.t1 MTR_8g020763 36.250 160 92 3 31 185 27 181 4.18e-21 86.7
MS.gene70063.t1 MTR_8g020760 34.715 193 114 5 4 194 24 206 1.87e-20 85.5
MS.gene70063.t1 MTR_2g019780 33.333 192 112 6 6 194 29 207 5.34e-20 84.0
MS.gene70063.t1 MTR_2g030915 49.351 77 23 1 71 147 1 61 3.15e-18 76.6
MS.gene70063.t1 MTR_4g027415 37.209 129 71 4 4 130 22 142 6.56e-15 68.9
MS.gene70063.t1 MTR_3g019810 33.516 182 102 8 3 181 127 292 9.21e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70063.t1 AT5G39150 63.918 194 67 2 3 194 26 218 7.30e-84 248
MS.gene70063.t1 AT5G39120 63.918 194 67 2 3 194 26 218 1.11e-83 247
MS.gene70063.t1 AT5G39180 63.918 194 67 2 3 194 26 218 1.33e-83 247
MS.gene70063.t1 AT3G05950 64.356 202 66 4 3 200 29 228 5.59e-83 246
MS.gene70063.t1 AT5G39110 63.402 194 68 2 3 194 27 219 4.26e-82 243
MS.gene70063.t1 AT5G39160 64.948 194 64 3 3 194 27 218 5.60e-82 243
MS.gene70063.t1 AT5G39190 64.948 194 64 3 3 194 27 218 9.26e-82 242
MS.gene70063.t1 AT5G39130 63.918 194 66 3 3 194 27 218 3.48e-81 241
MS.gene70063.t1 AT4G14630 61.856 194 71 2 3 194 29 221 7.46e-78 233
MS.gene70063.t1 AT5G39160 62.371 194 65 3 3 194 27 214 3.23e-77 231
MS.gene70063.t1 AT5G38940 59.585 193 74 2 4 194 30 220 2.73e-72 218
MS.gene70063.t1 AT5G38940 59.585 193 74 2 4 194 54 244 3.92e-72 219
MS.gene70063.t1 AT5G38930 60.622 193 72 2 4 194 30 220 5.99e-72 218
MS.gene70063.t1 AT3G04200 61.458 192 70 4 3 192 30 219 6.74e-72 218
MS.gene70063.t1 AT5G39190 58.854 192 57 2 3 194 27 196 3.15e-71 215
MS.gene70063.t1 AT5G39160 58.333 192 58 2 3 194 27 196 8.33e-71 214
MS.gene70063.t1 AT5G38960 57.949 195 77 3 3 194 29 221 6.61e-70 212
MS.gene70063.t1 AT5G38910 60.309 194 71 3 4 194 28 218 8.89e-70 212
MS.gene70063.t1 AT3G04190 54.167 192 84 3 3 192 29 218 9.10e-64 197
MS.gene70063.t1 AT3G04170 53.886 193 82 4 3 193 29 216 2.09e-63 196
MS.gene70063.t1 AT3G04150 54.404 193 84 3 3 193 29 219 7.14e-63 194
MS.gene70063.t1 AT3G04150 51.220 205 85 3 3 193 29 232 5.29e-61 190
MS.gene70063.t1 AT3G04180 52.604 192 87 3 3 192 29 218 1.69e-60 188
MS.gene70063.t1 AT1G02335 45.078 193 98 3 4 194 28 214 3.08e-49 159
MS.gene70063.t1 AT3G05930 49.724 181 82 3 4 181 27 201 3.22e-49 159
MS.gene70063.t1 AT1G09560 45.078 193 98 3 4 194 28 214 4.76e-49 159
MS.gene70063.t1 AT3G62020 46.392 194 95 4 4 194 27 214 7.78e-48 156
MS.gene70063.t1 AT5G26700 48.370 184 80 5 4 183 25 197 1.19e-47 155
MS.gene70063.t1 AT1G18970 44.330 194 98 4 3 194 32 217 1.84e-46 152
MS.gene70063.t1 AT1G18980 45.361 194 96 4 3 194 32 217 6.84e-46 151
MS.gene70063.t1 AT5G38910 47.423 194 59 3 4 194 28 181 8.49e-46 150
MS.gene70063.t1 AT3G62020 45.856 181 91 3 17 194 9 185 2.09e-44 146
MS.gene70063.t1 AT1G10460 39.594 197 110 5 3 194 22 214 4.69e-40 136
MS.gene70063.t1 AT3G10080 38.776 196 110 5 3 194 29 218 3.42e-39 134
MS.gene70063.t1 AT3G10080 38.776 196 110 5 3 194 100 289 1.56e-38 134
MS.gene70063.t1 AT5G20630 41.935 186 99 5 4 187 22 200 5.34e-36 125
MS.gene70063.t1 AT5G61750 42.073 164 89 2 4 165 23 182 1.48e-35 124
MS.gene70063.t1 AT1G72610 39.791 191 105 6 4 192 19 201 9.35e-32 114
MS.gene70063.t1 AT1G74820 37.629 194 111 4 3 194 39 224 2.55e-30 111
MS.gene70063.t1 AT5G38950 54.167 72 33 0 124 195 2 73 6.76e-18 75.9

Find 48 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATACATACCACCGTCTT+TGG 0.267702 4.2:+84946102 None:intergenic
ACGATTATTGTTGTCTTGAT+TGG 0.312169 4.2:+84945565 None:intergenic
GGCAATGCTGTTGCTATTGC+TGG 0.316712 4.2:-84945461 MS.gene70063:CDS
CTTATCCACTTGAAAAGCTT+TGG 0.335788 4.2:+84945355 None:intergenic
TTTCAGCTGAATGTGGGATA+TGG 0.348760 4.2:-84945482 MS.gene70063:CDS
TGAAAATGAATAAGACCAAT+TGG 0.356028 4.2:+84945500 None:intergenic
TAACGCACTCGGGATATCTT+TGG 0.364921 4.2:-84945696 MS.gene70063:CDS
GAGTGCCTTCAAGTACTATA+AGG 0.386700 4.2:+84945609 None:intergenic
ATATACATACCACCGTCTTT+GGG 0.386785 4.2:+84946103 None:intergenic
TTCTTCAAACATGTTGAAGC+TGG 0.402098 4.2:-84945782 MS.gene70063:CDS
GATGTGTTTGTGTTCCCAAT+TGG 0.424230 4.2:-84945515 MS.gene70063:CDS
TAATTTGCAGTATTCGTGAA+TGG 0.437869 4.2:-84945845 MS.gene70063:intron
ATTATCTTCAAAAGCAGTCT+TGG 0.442184 4.2:-84945322 MS.gene70063:CDS
CTTGAAGGCACTCTTTATGT+TGG 0.454430 4.2:-84945599 MS.gene70063:CDS
ATAAGGATCTCTGTTCCTCT+AGG 0.468428 4.2:+84945626 None:intergenic
GAGATCCTTATAGTACTTGA+AGG 0.469573 4.2:-84945614 MS.gene70063:CDS
GTCACTGTAGAACAACTATT+CGG 0.471205 4.2:-84945722 MS.gene70063:CDS
TGATTGCAACACAAAAGTCT+TGG 0.490396 4.2:+84946131 None:intergenic
ATCACCCTTGTTGAGCACTT+TGG 0.491510 4.2:+84945535 None:intergenic
AATTTGCAGTATTCGTGAAT+GGG 0.491761 4.2:-84945844 MS.gene70063:intron
TTGAGAGCCTAGTGCGTTGG+AGG 0.495388 4.2:+84945754 None:intergenic
CTCTAGGGTGAATGTGAGGT+GGG 0.498933 4.2:+84945642 None:intergenic
TCTTCAGCTTTAACAAGCGC+GGG 0.499389 4.2:+84945806 None:intergenic
TCTTCAAACATGTTGAAGCT+GGG 0.501315 4.2:-84945781 MS.gene70063:CDS
GGACTTAGCAGTCAAAATCT+AGG 0.508200 4.2:-84945440 MS.gene70063:CDS
GAAAATGAATAAGACCAATT+GGG 0.510087 4.2:+84945501 None:intergenic
AATAGTTGTTCTACAGTGAC+TGG 0.527501 4.2:+84945725 None:intergenic
TATTCGGACTTAACGCACTC+GGG 0.528628 4.2:-84945706 MS.gene70063:CDS
TGAGGTGGGTTTAAACCCTT+AGG 0.539104 4.2:+84945656 None:intergenic
AGCTTTGGTAAGAACTTCAT+CGG 0.541868 4.2:+84945370 None:intergenic
CACTTGAGAGCCTAGTGCGT+TGG 0.543021 4.2:+84945751 None:intergenic
ATCTTCAGCTTTAACAAGCG+CGG 0.545908 4.2:+84945805 None:intergenic
CTATTCGGACTTAACGCACT+CGG 0.553166 4.2:-84945707 MS.gene70063:CDS
GGGAATGCCTCCAACGCACT+AGG 0.554068 4.2:-84945761 MS.gene70063:CDS
TAAGGATCTCTGTTCCTCTA+GGG 0.579174 4.2:+84945627 None:intergenic
CCTCTAGGGTGAATGTGAGG+TGG 0.583739 4.2:+84945641 None:intergenic
TTTAAACAAAGCATTTGCAA+CGG 0.590900 4.2:+84945409 None:intergenic
CTTCACCAAAGTGCTCAACA+AGG 0.595909 4.2:-84945540 MS.gene70063:CDS
TATACATACCACCGTCTTTG+GGG 0.607682 4.2:+84946104 None:intergenic
GCAATCAAAGACCCCAAAGA+CGG 0.629437 4.2:-84946115 MS.gene70063:CDS
CAACACAAAAGTCTTGGAGA+GGG 0.634245 4.2:+84946137 None:intergenic
AGAACTTCATCGGAAATAAG+CGG 0.643482 4.2:+84945380 None:intergenic
TTCACCAAAGTGCTCAACAA+GGG 0.645837 4.2:-84945539 MS.gene70063:CDS
GTTCCTCTAGGGTGAATGTG+AGG 0.650118 4.2:+84945638 None:intergenic
GCAACACAAAAGTCTTGGAG+AGG 0.651085 4.2:+84946136 None:intergenic
CTTCAGCTTTAACAAGCGCG+GGG 0.662073 4.2:+84945807 None:intergenic
ATCAAAGACCCCAAAGACGG+TGG 0.676908 4.2:-84946112 MS.gene70063:intron
CCACCTCACATTCACCCTAG+AGG 0.685266 4.2:-84945641 MS.gene70063:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTCTATAAATATATTTTTA+TGG - chr4.2:84945712-84945731 MS.gene70063:CDS 10.0%
!!! ATATATAGAATCTTTTGTTT+TGG - chr4.2:84945748-84945767 MS.gene70063:CDS 15.0%
!!! TTAATTACTACTCAATTTTT+AGG - chr4.2:84945641-84945660 MS.gene70063:CDS 15.0%
!! TAATTAAGACTTAAGAGTTA+AGG + chr4.2:84945628-84945647 None:intergenic 20.0%
! CAAAACGTTTGATATTTGTA+AGG + chr4.2:84945685-84945704 None:intergenic 25.0%
! GAAAATGAATAAGACCAATT+GGG + chr4.2:84946204-84946223 None:intergenic 25.0%
! TGAAAATGAATAAGACCAAT+TGG + chr4.2:84946205-84946224 None:intergenic 25.0%
!! TTTAAACAAAGCATTTGCAA+CGG + chr4.2:84946296-84946315 None:intergenic 25.0%
AATTTGCAGTATTCGTGAAT+GGG - chr4.2:84945858-84945877 MS.gene70063:intron 30.0%
ACGATTATTGTTGTCTTGAT+TGG + chr4.2:84946140-84946159 None:intergenic 30.0%
ATTATCTTCAAAAGCAGTCT+TGG - chr4.2:84946380-84946399 MS.gene70063:intron 30.0%
! ATTCATTTTCAGCTGAATGT+GGG - chr4.2:84946214-84946233 MS.gene70063:intron 30.0%
! TAATTTGCAGTATTCGTGAA+TGG - chr4.2:84945857-84945876 MS.gene70063:intron 30.0%
! TATTCATTTTCAGCTGAATG+TGG - chr4.2:84946213-84946232 MS.gene70063:intron 30.0%
!! TTAATTAGCATCTTGGCTTT+TGG - chr4.2:84945511-84945530 MS.gene70063:CDS 30.0%
AATAGTTGTTCTACAGTGAC+TGG + chr4.2:84945980-84945999 None:intergenic 35.0%
AGAACTTCATCGGAAATAAG+CGG + chr4.2:84946325-84946344 None:intergenic 35.0%
AGCCAAGATGCTAATTAACA+AGG + chr4.2:84945509-84945528 None:intergenic 35.0%
ATATACATACCACCGTCTTT+GGG + chr4.2:84945602-84945621 None:intergenic 35.0%
CAAGATGCTAATTAACAAGG+AGG + chr4.2:84945506-84945525 None:intergenic 35.0%
CTCCTTGTTAATTAGCATCT+TGG - chr4.2:84945504-84945523 MS.gene70063:CDS 35.0%
CTTATCCACTTGAAAAGCTT+TGG + chr4.2:84946350-84946369 None:intergenic 35.0%
GAGATCCTTATAGTACTTGA+AGG - chr4.2:84946088-84946107 MS.gene70063:intron 35.0%
GTCACTGTAGAACAACTATT+CGG - chr4.2:84945980-84945999 MS.gene70063:intron 35.0%
TATATACATACCACCGTCTT+TGG + chr4.2:84945603-84945622 None:intergenic 35.0%
TCTTCAAACATGTTGAAGCT+GGG - chr4.2:84945921-84945940 MS.gene70063:intron 35.0%
TGATTGCAACACAAAAGTCT+TGG + chr4.2:84945574-84945593 None:intergenic 35.0%
TTCTTCAAACATGTTGAAGC+TGG - chr4.2:84945920-84945939 MS.gene70063:intron 35.0%
! AGCTTTGGTAAGAACTTCAT+CGG + chr4.2:84946335-84946354 None:intergenic 35.0%
!! TCTTACCAAAGCTTTTCAAG+TGG - chr4.2:84946342-84946361 MS.gene70063:intron 35.0%
ATAAGGATCTCTGTTCCTCT+AGG + chr4.2:84946079-84946098 None:intergenic 40.0%
CAACACAAAAGTCTTGGAGA+GGG + chr4.2:84945568-84945587 None:intergenic 40.0%
GAGTGCCTTCAAGTACTATA+AGG + chr4.2:84946096-84946115 None:intergenic 40.0%
GATGTGTTTGTGTTCCCAAT+TGG - chr4.2:84946187-84946206 MS.gene70063:intron 40.0%
GGACTTAGCAGTCAAAATCT+AGG - chr4.2:84946262-84946281 MS.gene70063:intron 40.0%
TAAGGATCTCTGTTCCTCTA+GGG + chr4.2:84946078-84946097 None:intergenic 40.0%
TATACATACCACCGTCTTTG+GGG + chr4.2:84945601-84945620 None:intergenic 40.0%
TTTCAGCTGAATGTGGGATA+TGG - chr4.2:84946220-84946239 MS.gene70063:intron 40.0%
! ATCTTCAGCTTTAACAAGCG+CGG + chr4.2:84945900-84945919 None:intergenic 40.0%
! TTCACCAAAGTGCTCAACAA+GGG - chr4.2:84946163-84946182 MS.gene70063:intron 40.0%
!! CTTGAAGGCACTCTTTATGT+TGG - chr4.2:84946103-84946122 MS.gene70063:intron 40.0%
AGCTAAACCAGCAATCACTG+AGG + chr4.2:84945775-84945794 None:intergenic 45.0%
ATCACCCTTGTTGAGCACTT+TGG + chr4.2:84946170-84946189 None:intergenic 45.0%
CTATTCGGACTTAACGCACT+CGG - chr4.2:84945995-84946014 MS.gene70063:intron 45.0%
GCAACACAAAAGTCTTGGAG+AGG + chr4.2:84945569-84945588 None:intergenic 45.0%
GCAATCAAAGACCCCAAAGA+CGG - chr4.2:84945587-84945606 MS.gene70063:CDS 45.0%
TAACGCACTCGGGATATCTT+TGG - chr4.2:84946006-84946025 MS.gene70063:intron 45.0%
TATTCGGACTTAACGCACTC+GGG - chr4.2:84945996-84946015 MS.gene70063:intron 45.0%
! CTTCACCAAAGTGCTCAACA+AGG - chr4.2:84946162-84946181 MS.gene70063:intron 45.0%
! TCTTCAGCTTTAACAAGCGC+GGG + chr4.2:84945899-84945918 None:intergenic 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG + chr4.2:84946049-84946068 None:intergenic 45.0%
! TTTTGGTCCTCAGTGATTGC+TGG - chr4.2:84945765-84945784 MS.gene70063:CDS 45.0%
!! CAGTGATTGCTGGTTTAGCT+AGG - chr4.2:84945775-84945794 MS.gene70063:CDS 45.0%
AAAAGTCTTGGAGAGGGCTG+GGG + chr4.2:84945562-84945581 None:intergenic 50.0%
ACAAAAGTCTTGGAGAGGGC+TGG + chr4.2:84945564-84945583 None:intergenic 50.0%
ATCAAAGACCCCAAAGACGG+TGG - chr4.2:84945590-84945609 MS.gene70063:CDS 50.0%
CAAAAGTCTTGGAGAGGGCT+GGG + chr4.2:84945563-84945582 None:intergenic 50.0%
GTCAGAAGCAAAGGCAAGAG+AGG + chr4.2:84945540-84945559 None:intergenic 50.0%
! CTTCAGCTTTAACAAGCGCG+GGG + chr4.2:84945898-84945917 None:intergenic 50.0%
! GGCAATGCTGTTGCTATTGC+TGG - chr4.2:84946241-84946260 MS.gene70063:intron 50.0%
!! CTCTAGGGTGAATGTGAGGT+GGG + chr4.2:84946063-84946082 None:intergenic 50.0%
!! GTTCCTCTAGGGTGAATGTG+AGG + chr4.2:84946067-84946086 None:intergenic 50.0%
!!! CGCGTTGATTTTGCGCCTAA+GGG - chr4.2:84946031-84946050 MS.gene70063:intron 50.0%
!!! TCGCGTTGATTTTGCGCCTA+AGG - chr4.2:84946030-84946049 MS.gene70063:intron 50.0%
CACTTGAGAGCCTAGTGCGT+TGG + chr4.2:84945954-84945973 None:intergenic 55.0%
CCACCTCACATTCACCCTAG+AGG - chr4.2:84946061-84946080 MS.gene70063:intron 55.0%
TTGAGAGCCTAGTGCGTTGG+AGG + chr4.2:84945951-84945970 None:intergenic 55.0%
!! CCTCTAGGGTGAATGTGAGG+TGG + chr4.2:84946064-84946083 None:intergenic 55.0%
AGGGCTGGGGTCAGAAGCAA+AGG + chr4.2:84945549-84945568 None:intergenic 60.0%
GGGAATGCCTCCAACGCACT+AGG - chr4.2:84945941-84945960 MS.gene70063:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 84945307 84946417 84945307 ID=MS.gene70063
chr4.2 mRNA 84945307 84946417 84945307 ID=MS.gene70063.t1;Parent=MS.gene70063
chr4.2 exon 84946413 84946417 84946413 ID=MS.gene70063.t1.exon1;Parent=MS.gene70063.t1
chr4.2 CDS 84946413 84946417 84946413 ID=cds.MS.gene70063.t1;Parent=MS.gene70063.t1
chr4.2 exon 84946113 84946159 84946113 ID=MS.gene70063.t1.exon2;Parent=MS.gene70063.t1
chr4.2 CDS 84946113 84946159 84946113 ID=cds.MS.gene70063.t1;Parent=MS.gene70063.t1
chr4.2 exon 84945307 84945857 84945307 ID=MS.gene70063.t1.exon3;Parent=MS.gene70063.t1
chr4.2 CDS 84945307 84945857 84945307 ID=cds.MS.gene70063.t1;Parent=MS.gene70063.t1
Gene Sequence

>MS.gene70063

ATGTAGTTGTTAAAGAATGGTTCATTATTTAATAAAAAATTAATGACGATAAAGATCAAATTAAATGCAAACAACTATAATATTTCATTCTTATAAATAGCAAGCAAATACATTCAAGAAAACTCAATTCTCACAAGTTATTTACATAAGAAGAATATTAAAGAGAAATACTGAGTTGCAAAATGAAGATTTCATACCTCCTTGTTAATTAGCATCTTGGCTTTTGGCATCCTCTCTTGCCTTTGCTTCTGACCCCAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGACGGTGGTATGTATATATTCCTTAACTCTTAAGTCTTAATTACTACTCAATTTTTAGGCTTAAATATACAAATGCTCCTTACAAATATCAAACGTTTTGATTTTAGTCTCTATAAATATATTTTTATGGTTTTAGTTCTTGTATATATAGAATCTTTTGTTTTGGTCCTCAGTGATTGCTGGTTTAGCTAGGTCATGACTAGAAGAATTATACTACTGAAATTTCTTTGATATAACAAAATTATGATTTGATAATTTGCAGTATTCGTGAATGGGAAATTCTATAAAGACCCCGCGCTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCCAACGCACTAGGCTCTCAAGTGACTCCAGTCACTGTAGAACAACTATTCGGACTTAACGCACTCGGGATATCTTTGGCTCGCGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATTCACCCTAGAGGAACAGAGATCCTTATAGTACTTGAAGGCACTCTTTATGTTGGATTTGTCACGTCCAATCAAGACAACAATAATCGTCTCTTCACCAAAGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAGCTGAATGTGGGATATGGCAATGCTGTTGCTATTGCTGGACTTAGCAGTCAAAATCTAGGAGTTATCACCGTTGCAAATGCTTTGTTTAAATCTAATCCGCTTATTTCCGATGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG
Protein sequence

>MS.gene70063.t1

MYPLQDFCVAIKDPKDGVFVNGKFYKDPALVKAEDFFKHVEAGNASNALGSQVTPVTVEQLFGLNALGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVTSNQDNNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNLGVITVANALFKSNPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN