AlfalfaGEDB | MS.gene82858

M. sativa cultivar XinJiangDaYe / MS.gene82858

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82858.t1	XP_003618165.1	97.3	220	6	0	1	220	1	220	4.30E-117	430.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82858.t1	Q9FIC9	64.8	219	73	3	1	215	1	219	4.7e-72	272.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82858.t1	G7KQ15	97.7	219	5	0	1	219	1	219	3.1e-117	430.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene82858	0.804028	2.63E-49	-1.69E-46
MS.gene049549	MS.gene82858	0.840324	9.31E-58	-1.69E-46
MS.gene049611	MS.gene82858	0.805894	1.07E-49	-1.69E-46
MS.gene049805	MS.gene82858	-0.800596	1.34E-48	-1.69E-46
MS.gene050095	MS.gene82858	0.801064	1.07E-48	-1.69E-46
MS.gene050096	MS.gene82858	0.802316	5.94E-49	-1.69E-46
MS.gene050381	MS.gene82858	0.808064	3.72E-50	-1.69E-46
MS.gene050593	MS.gene82858	-0.802165	6.38E-49	-1.69E-46
MS.gene050647	MS.gene82858	0.854187	1.45E-61	-1.69E-46
MS.gene050670	MS.gene82858	-0.800462	1.42E-48	-1.69E-46
MS.gene050747	MS.gene82858	-0.805308	1.42E-49	-1.69E-46
MS.gene050809	MS.gene82858	0.802373	5.79E-49	-1.69E-46
MS.gene050950	MS.gene82858	-0.800713	1.27E-48	-1.69E-46
MS.gene05104	MS.gene82858	0.818192	2.23E-52	-1.69E-46
MS.gene051127	MS.gene82858	0.811821	5.78E-51	-1.69E-46
MS.gene051666	MS.gene82858	-0.802204	6.27E-49	-1.69E-46
MS.gene051694	MS.gene82858	-0.812169	4.85E-51	-1.69E-46
MS.gene051907	MS.gene82858	0.805994	1.02E-49	-1.69E-46
MS.gene052211	MS.gene82858	0.818485	1.91E-52	-1.69E-46
MS.gene05240	MS.gene82858	0.843962	1.01E-58	-1.69E-46
MS.gene05244	MS.gene82858	0.806277	8.90E-50	-1.69E-46
MS.gene052606	MS.gene82858	0.82338	1.43E-53	-1.69E-46
MS.gene052615	MS.gene82858	-0.801341	9.43E-49	-1.69E-46
MS.gene052805	MS.gene82858	0.809119	2.21E-50	-1.69E-46
MS.gene053030	MS.gene82858	0.820833	5.57E-53	-1.69E-46
MS.gene053031	MS.gene82858	0.859518	3.91E-63	-1.69E-46
MS.gene053033	MS.gene82858	0.878032	3.90E-69	-1.69E-46
MS.gene053406	MS.gene82858	0.825895	3.65E-54	-1.69E-46
MS.gene053420	MS.gene82858	0.800464	1.42E-48	-1.69E-46
MS.gene054146	MS.gene82858	0.836109	1.13E-56	-1.69E-46
MS.gene054147	MS.gene82858	0.814474	1.51E-51	-1.69E-46
MS.gene054290	MS.gene82858	0.848914	4.52E-60	-1.69E-46
MS.gene054421	MS.gene82858	0.808513	2.99E-50	-1.69E-46
MS.gene054742	MS.gene82858	0.842552	2.41E-58	-1.69E-46
MS.gene055124	MS.gene82858	0.82264	2.13E-53	-1.69E-46
MS.gene055601	MS.gene82858	0.824479	7.89E-54	-1.69E-46
MS.gene056330	MS.gene82858	0.812384	4.36E-51	-1.69E-46
MS.gene056590	MS.gene82858	0.807432	5.07E-50	-1.69E-46
MS.gene056591	MS.gene82858	0.807212	5.65E-50	-1.69E-46
MS.gene056748	MS.gene82858	0.809155	2.18E-50	-1.69E-46
MS.gene056856	MS.gene82858	0.801652	8.14E-49	-1.69E-46
MS.gene056899	MS.gene82858	0.804153	2.48E-49	-1.69E-46
MS.gene056920	MS.gene82858	0.823201	1.57E-53	-1.69E-46
MS.gene056922	MS.gene82858	0.807779	4.28E-50	-1.69E-46
MS.gene057146	MS.gene82858	-0.809362	1.96E-50	-1.69E-46
MS.gene057544	MS.gene82858	0.818135	2.30E-52	-1.69E-46
MS.gene05837	MS.gene82858	-0.812122	4.97E-51	-1.69E-46
MS.gene058603	MS.gene82858	0.810001	1.43E-50	-1.69E-46
MS.gene059037	MS.gene82858	0.811479	6.86E-51	-1.69E-46
MS.gene059402	MS.gene82858	0.803822	2.90E-49	-1.69E-46
MS.gene059536	MS.gene82858	-0.8093	2.03E-50	-1.69E-46
MS.gene059955	MS.gene82858	0.812105	5.01E-51	-1.69E-46
MS.gene060087	MS.gene82858	0.808323	3.28E-50	-1.69E-46
MS.gene060593	MS.gene82858	0.806753	7.06E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene82858	MS.gene50786	PPI
MS.gene82858	MS.gene053031	PPI
MS.gene82858	MS.gene50789	PPI
MS.gene82858	MS.gene70063	PPI
MS.gene032598	MS.gene82858	PPI
MS.gene89773	MS.gene82858	PPI
MS.gene82858	MS.gene50788	PPI
MS.gene053033	MS.gene82858	PPI
MS.gene82858	MS.gene037520	PPI
MS.gene82858	MS.gene053030	PPI
MS.gene053027	MS.gene82858	PPI
MS.gene74887	MS.gene82858	PPI
MS.gene032599	MS.gene82858	PPI
MS.gene82858	MS.gene82855	PPI
MS.gene82858	MS.gene70061	PPI
MS.gene82858	MS.gene032599	PPI
MS.gene82858	MS.gene002728	PPI
MS.gene82858	MS.gene032596	PPI
MS.gene053030	MS.gene82858	PPI
MS.gene82858	MS.gene037518	PPI
MS.gene82858	MS.gene50787	PPI
MS.gene82858	MS.gene037519	PPI
MS.gene82858	MS.gene053029	PPI
MS.gene82858	MS.gene70064	PPI
MS.gene82858	MS.gene047464	PPI
MS.gene82858	MS.gene89774	PPI
MS.gene82858	MS.gene82860	PPI
MS.gene89775	MS.gene82858	PPI
MS.gene82858	MS.gene053026	PPI
MS.gene82858	MS.gene032597	PPI
MS.gene82858	MS.gene82857	PPI
MS.gene82858	MS.gene037516	PPI
MS.gene032601	MS.gene82858	PPI
MS.gene82858	MS.gene005041	PPI
MS.gene82858	MS.gene82859	PPI
MS.gene82858	MS.gene89772	PPI
MS.gene82858	MS.gene037517	PPI
MS.gene82858	MS.gene07591	PPI
MS.gene037517	MS.gene82858	PPI
MS.gene82858	MS.gene70062	PPI
MS.gene70064	MS.gene82858	PPI
MS.gene82858	MS.gene50790	PPI
MS.gene82858	MS.gene96244	PPI
MS.gene82858	MS.gene004411	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82858.t1	MTR_6g005310	97.717	219	5	0	1	219	1	219	1.58e-156	432
MS.gene82858.t1	MTR_6g005340	97.273	220	6	0	1	220	1	220	1.81e-156	432
MS.gene82858.t1	MTR_6g005360	96.364	220	8	0	1	220	1	220	6.05e-155	428
MS.gene82858.t1	MTR_6g005330	95.000	220	11	0	1	220	1	220	2.19e-152	422
MS.gene82858.t1	MTR_1g079490	83.410	217	33	3	7	220	6	222	2.80e-127	358
MS.gene82858.t1	MTR_4g017050	79.358	218	43	2	5	220	4	221	6.75e-122	345
MS.gene82858.t1	MTR_5g046410	79.358	218	43	2	5	220	4	221	7.21e-122	345
MS.gene82858.t1	MTR_5g046430	79.358	218	43	2	5	220	4	221	7.21e-122	345
MS.gene82858.t1	MTR_4g017030	79.358	218	43	2	5	220	4	221	7.21e-122	345
MS.gene82858.t1	MTR_4g017040	77.828	221	48	1	1	220	1	221	7.53e-122	345
MS.gene82858.t1	MTR_2g019250	78.899	218	44	2	5	220	4	221	1.01e-121	344
MS.gene82858.t1	MTR_6g005350	74.091	220	56	1	1	220	1	219	2.33e-112	320
MS.gene82858.t1	MTR_6g005380	71.154	208	59	1	1	207	1	208	1.58e-102	295
MS.gene82858.t1	MTR_1g023770	64.322	199	61	2	19	215	19	209	4.23e-86	254
MS.gene82858.t1	MTR_2g030855	63.819	199	65	2	19	215	19	212	3.61e-85	251
MS.gene82858.t1	MTR_2g031270	63.500	200	65	3	19	215	19	213	1.48e-82	245
MS.gene82858.t1	MTR_2g030895	66.860	172	54	2	47	215	7	178	1.12e-77	231
MS.gene82858.t1	MTR_2g030865	66.860	172	54	2	47	215	7	178	2.96e-76	228
MS.gene82858.t1	MTR_2g031300	60.870	161	41	2	47	205	7	147	1.51e-60	187
MS.gene82858.t1	MTR_1g085500	46.190	210	103	4	11	214	8	213	1.45e-58	184
MS.gene82858.t1	MTR_7g111240	50.510	196	91	3	22	215	20	211	1.06e-55	177
MS.gene82858.t1	MTR_1g085140	47.500	200	99	3	18	215	20	215	8.77e-55	174
MS.gene82858.t1	MTR_5g036570	48.731	197	94	4	22	215	27	219	1.42e-53	171
MS.gene82858.t1	MTR_7g111240	50.000	196	89	3	22	215	20	208	2.86e-53	170
MS.gene82858.t1	MTR_1g112200	43.056	216	108	6	2	215	5	207	2.85e-47	155
MS.gene82858.t1	MTR_2g041550	42.056	214	109	5	1	212	3	203	3.61e-43	144
MS.gene82858.t1	MTR_1g060120	39.048	210	118	4	12	215	16	221	8.49e-43	144
MS.gene82858.t1	MTR_8g020750	44.103	195	90	5	1	189	3	184	2.67e-42	142
MS.gene82858.t1	MTR_2g041570	39.070	215	116	5	1	213	3	204	3.72e-39	134
MS.gene82858.t1	MTR_2g041580	39.252	214	115	5	1	212	3	203	4.61e-39	134
MS.gene82858.t1	MTR_8g020590	40.000	190	106	4	26	213	19	202	1.07e-35	125
MS.gene82858.t1	MTR_8g020630	37.736	212	119	5	5	215	6	205	3.47e-35	124
MS.gene82858.t1	MTR_3g019710	38.679	212	117	5	5	215	6	205	5.81e-35	123
MS.gene82858.t1	MTR_8g007300	41.071	168	96	2	22	188	26	191	1.02e-34	123
MS.gene82858.t1	MTR_8g020650	40.212	189	105	4	28	215	24	205	3.93e-34	121
MS.gene82858.t1	MTR_3g019680	38.208	212	117	5	5	215	6	204	5.34e-34	121
MS.gene82858.t1	MTR_3g019650	37.736	212	118	5	5	215	6	204	1.61e-33	119
MS.gene82858.t1	MTR_2g045100	38.208	212	118	5	5	215	6	205	3.11e-33	119
MS.gene82858.t1	MTR_2g036120	37.688	199	116	4	18	215	14	205	3.32e-32	116
MS.gene82858.t1	MTR_8g020670	36.449	214	123	6	3	215	4	205	2.16e-31	114
MS.gene82858.t1	MTR_8g020770	37.788	217	114	6	5	215	7	208	4.11e-31	114
MS.gene82858.t1	MTR_4g010340	39.655	174	98	4	43	215	34	201	5.87e-31	113
MS.gene82858.t1	MTR_2g044040	36.683	199	118	4	18	215	14	205	1.16e-30	112
MS.gene82858.t1	MTR_8g020610	40.000	190	104	6	28	215	22	203	1.33e-30	112
MS.gene82858.t1	MTR_8g020760	34.906	212	130	4	5	215	3	207	5.61e-29	108
MS.gene82858.t1	MTR_0284s0010	35.377	212	124	5	5	215	6	205	6.27e-29	108
MS.gene82858.t1	MTR_5g031460	34.673	199	122	4	18	215	14	205	2.23e-28	106
MS.gene82858.t1	MTR_2g019780	35.023	217	130	6	1	215	1	208	3.91e-28	105
MS.gene82858.t1	MTR_3g096330	33.488	215	136	4	1	211	553	764	5.54e-28	111
MS.gene82858.t1	MTR_7g011340	36.649	191	113	4	26	215	22	205	8.61e-28	105
MS.gene82858.t1	MTR_8g020763	36.000	175	105	3	43	215	17	186	3.71e-27	102
MS.gene82858.t1	MTR_4g052770	39.860	143	81	3	74	215	67	205	1.03e-26	102
MS.gene82858.t1	MTR_2g086620	34.842	221	113	8	2	215	8	204	1.34e-26	102
MS.gene82858.t1	MTR_4g052780	39.860	143	81	3	74	215	67	205	1.64e-26	101
MS.gene82858.t1	MTR_2g086630	35.023	217	110	8	6	215	12	204	1.79e-26	101
MS.gene82858.t1	MTR_3g073170	36.508	189	110	5	28	215	27	206	7.87e-26	100
MS.gene82858.t1	MTR_2g086640	34.419	215	122	7	2	215	7	203	1.52e-25	99.0
MS.gene82858.t1	MTR_2g030915	54.667	75	20	1	93	167	1	61	2.81e-20	82.4
MS.gene82858.t1	MTR_3g019810	30.986	213	129	8	2	212	106	302	2.45e-17	79.3
MS.gene82858.t1	MTR_4g027415	39.683	126	70	3	26	150	22	142	8.25e-17	74.3
MS.gene82858.t1	MTR_2g072560	40.385	104	59	2	18	120	14	115	2.73e-13	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82858.t1	AT5G39120	64.840	219	73	3	1	215	1	219	3.16e-94	275
MS.gene82858.t1	AT5G39150	64.384	219	74	3	1	215	1	219	2.50e-93	272
MS.gene82858.t1	AT5G39180	64.384	219	74	3	1	215	1	219	3.87e-93	272
MS.gene82858.t1	AT5G39110	64.651	215	73	2	4	215	6	220	1.30e-92	271
MS.gene82858.t1	AT5G38940	67.347	196	62	1	22	215	26	221	4.28e-91	267
MS.gene82858.t1	AT5G38940	67.347	196	62	1	22	215	50	245	7.58e-91	267
MS.gene82858.t1	AT5G38930	67.677	198	62	1	20	215	24	221	3.16e-90	265
MS.gene82858.t1	AT5G39130	66.332	199	63	3	20	215	22	219	2.67e-89	262
MS.gene82858.t1	AT3G05950	66.986	209	64	4	11	215	14	221	2.92e-89	262
MS.gene82858.t1	AT5G39160	66.834	199	62	3	20	215	22	219	2.97e-89	262
MS.gene82858.t1	AT5G39190	66.834	199	62	3	20	215	22	219	4.97e-89	261
MS.gene82858.t1	AT5G38910	63.470	219	76	4	1	215	1	219	8.92e-88	258
MS.gene82858.t1	AT5G38960	64.677	201	68	2	18	215	22	222	1.02e-87	258
MS.gene82858.t1	AT4G14630	64.824	199	67	2	20	215	24	222	5.04e-86	254
MS.gene82858.t1	AT5G39160	64.322	199	63	3	20	215	22	215	1.46e-84	250
MS.gene82858.t1	AT3G04200	62.500	216	74	4	3	215	11	222	2.62e-83	247
MS.gene82858.t1	AT3G04150	58.108	222	85	5	1	215	1	221	8.78e-77	231
MS.gene82858.t1	AT5G39190	59.898	197	57	2	20	215	22	197	3.81e-75	226
MS.gene82858.t1	AT5G39160	59.391	197	58	2	20	215	22	197	1.10e-74	224
MS.gene82858.t1	AT3G04150	54.701	234	87	5	1	215	1	234	1.71e-74	225
MS.gene82858.t1	AT3G04190	58.824	204	80	3	15	215	19	221	1.93e-74	224
MS.gene82858.t1	AT3G04170	57.416	209	81	5	11	215	14	218	3.28e-72	219
MS.gene82858.t1	AT3G04180	56.373	204	85	3	15	215	19	221	6.33e-71	216
MS.gene82858.t1	AT5G38910	51.598	219	65	5	1	215	1	182	3.74e-61	189
MS.gene82858.t1	AT1G09560	49.239	197	94	3	22	216	24	216	8.69e-57	179
MS.gene82858.t1	AT1G02335	46.970	198	99	3	20	215	22	215	1.72e-56	179
MS.gene82858.t1	AT3G05930	45.455	220	110	5	1	215	1	215	1.65e-55	176
MS.gene82858.t1	AT3G62020	46.698	212	106	4	7	215	8	215	1.90e-55	176
MS.gene82858.t1	AT1G18970	46.479	213	106	4	7	217	14	220	2.87e-55	176
MS.gene82858.t1	AT1G18980	48.990	198	93	4	22	217	29	220	3.30e-53	171
MS.gene82858.t1	AT5G26700	45.933	209	94	6	11	215	16	209	2.73e-52	168
MS.gene82858.t1	AT1G10460	42.714	199	107	5	22	215	19	215	3.34e-48	157
MS.gene82858.t1	AT3G62020	47.778	180	89	3	39	215	9	186	4.11e-48	156
MS.gene82858.t1	AT3G10080	40.796	201	111	4	19	215	23	219	9.42e-44	146
MS.gene82858.t1	AT3G10080	40.594	202	112	4	18	215	93	290	4.69e-43	147
MS.gene82858.t1	AT5G20630	41.361	191	100	4	1	189	3	183	1.07e-39	135
MS.gene82858.t1	AT5G61750	42.703	185	96	4	5	187	7	183	4.34e-39	134
MS.gene82858.t1	AT1G74820	38.073	218	119	5	8	215	14	225	6.59e-36	126
MS.gene82858.t1	AT1G72610	38.624	189	108	4	26	212	19	201	3.21e-33	119
MS.gene82858.t1	AT5G38950	65.625	64	22	0	144	207	2	65	3.01e-21	85.1
MS.gene82858.t1	AT5G39100	66.038	53	18	0	4	56	6	58	1.16e-17	74.3

Find 49 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGTTTGTGACAGCCTTCT+TGG	0.121595	6.1:+1298724	MS.gene82858:CDS
ACTATTGCTAATGCTGTATT+TGG	0.123951	6.1:+1299426	MS.gene82858:CDS
TAGTGATAACACCTGGATTT+TGG	0.245631	6.1:-1299407	None:intergenic
GGAAATGCGGTTGCCATTGC+TGG	0.324286	6.1:+1299375	MS.gene82858:CDS
AGTATACATACCACCATTCT+TGG	0.343654	6.1:-1298816	None:intergenic
CTTGTCCACTTGAAAAGCTT+TGG	0.353600	6.1:-1299481	None:intergenic
TCATTTGCTGTAGCAAGCTT+TGG	0.355521	6.1:-1299036	None:intergenic
CTCGTGTTGACTTTGCATCT+AGG	0.405287	6.1:+1299169	MS.gene82858:CDS
TTCCATACCCCACATTCAAT+TGG	0.409845	6.1:-1299356	None:intergenic
TGGAAGTGAATGAGACCAAT+TGG	0.411740	6.1:-1299336	None:intergenic
GATGTATTTGTGTTTCCAAT+TGG	0.425807	6.1:+1299321	MS.gene82858:CDS
TCGTGTTGACTTTGCATCTA+GGG	0.430505	6.1:+1299170	MS.gene82858:CDS
AGTATCTCATTAACAGTAAC+CGG	0.431864	6.1:-1299117	None:intergenic
TCAAGTGGACAAGAATATAG+TGG	0.453092	6.1:+1299491	MS.gene82858:CDS
ACAAAAGTCTTGTAGAGGGC+TGG	0.457530	6.1:-1298777	None:intergenic
TTCTTCTACTCGGTGAAAGA+AGG	0.467010	6.1:+1299060	MS.gene82858:CDS
ATAAGAATCTCAGTGGCTCT+AGG	0.470121	6.1:-1299216	None:intergenic
CAAAAGTCTTGTAGAGGGCT+GGG	0.476086	6.1:-1298776	None:intergenic
TGAGATTCTTATCGTGTTGG+AGG	0.479548	6.1:+1299227	MS.gene82858:CDS
TTCCAATTGAATGTGGGGTA+TGG	0.505417	6.1:+1299354	MS.gene82858:CDS
CATTCACTTCCAATTGAATG+TGG	0.512147	6.1:+1299347	MS.gene82858:CDS
TTGTAGAGGGCTGGGATCAA+AGG	0.523699	6.1:-1298768	None:intergenic
ATTCACTTCCAATTGAATGT+GGG	0.537723	6.1:+1299348	MS.gene82858:CDS
CACTGAGATTCTTATCGTGT+TGG	0.542092	6.1:+1299224	MS.gene82858:CDS
GTGGCTCTAGGGTGAGTATG+TGG	0.548107	6.1:-1299204	None:intergenic
TAAGAATCTCAGTGGCTCTA+GGG	0.548530	6.1:-1299215	None:intergenic
GGACTTAGCAGCCAAAATCC+AGG	0.549743	6.1:+1299396	MS.gene82858:CDS
TTCACTTCCAATTGAATGTG+GGG	0.551943	6.1:+1299349	MS.gene82858:CDS
AGCAAATGATTTCTTCTACT+CGG	0.553402	6.1:+1299050	MS.gene82858:CDS
ATACTAGGACTAAACACTCT+AGG	0.572011	6.1:+1299135	MS.gene82858:CDS
ACAAGATGCTAAAGCCAAGA+AGG	0.574992	6.1:-1298738	None:intergenic
GTTACTGTTAATGAGATACT+AGG	0.582511	6.1:+1299120	MS.gene82858:CDS
TGTGAATGGCAAATTCTGCA+AGG	0.584711	6.1:+1299011	MS.gene82858:CDS
CGTGTTGACTTTGCATCTAG+GGG	0.592403	6.1:+1299171	MS.gene82858:CDS
TTGGAGGGTACTCTATATGT+CGG	0.592575	6.1:+1299243	MS.gene82858:CDS
TTGGCTGCTAAGTCCAGCAA+TGG	0.600072	6.1:-1299388	None:intergenic
GCCACACAAAAGTCTTGTAG+AGG	0.603085	6.1:-1298782	None:intergenic
GAGATTCTTATCGTGTTGGA+GGG	0.612927	6.1:+1299228	MS.gene82858:CDS
ATCAATGACACCAAGAATGG+TGG	0.627435	6.1:+1298806	MS.gene82858:CDS
GCTATCAATGACACCAAGAA+TGG	0.633041	6.1:+1298803	MS.gene82858:CDS
GGAAATACATCAAATCCACT+TGG	0.640192	6.1:+1299081	MS.gene82858:CDS
AGGGCTGGGATCAAAGGCAA+AGG	0.641928	6.1:-1298762	None:intergenic
GAATGTGGGGTATGGAAATG+CGG	0.644018	6.1:+1299362	MS.gene82858:CDS
ATCACAAAAGTACTCAACAA+AGG	0.644517	6.1:+1299297	MS.gene82858:CDS
TTAGCAATAGTGATAACACC+TGG	0.654937	6.1:-1299414	None:intergenic
CCACACAAAAGTCTTGTAGA+GGG	0.655758	6.1:-1298781	None:intergenic
ACTTGGTTCAAAAGTGACAC+CGG	0.669513	6.1:+1299098	MS.gene82858:CDS
GCTCTAGGGTGAGTATGTGG+AGG	0.673911	6.1:-1299201	None:intergenic
CAACACGATAAGAATCTCAG+TGG	0.757588	6.1:-1299223	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGTATATATCAATGTTGT+CGG	-	chr6.1:1298940-1298959	None:intergenic	20.0%
!!!	ACAATCTTCAAAAACAATTT+TGG	+	chr6.1:1299514-1299533	MS.gene82858:CDS	20.0%
!!!	CAATCTTCAAAAACAATTTT+GGG	+	chr6.1:1299515-1299534	MS.gene82858:CDS	20.0%
!	TCATAGTTCAATCAGTTTAA+CGG	-	chr6.1:1298887-1298906	None:intergenic	25.0%
!	TTTATTCATCTTTACTCGTT+TGG	+	chr6.1:1298855-1298874	MS.gene82858:intron	25.0%
	ACTATGATACAAAAGTCTCA+CGG	+	chr6.1:1298900-1298919	MS.gene82858:intron	30.0%
	ACTATTGCTAATGCTGTATT+TGG	+	chr6.1:1299426-1299445	MS.gene82858:CDS	30.0%
	AGCAAATGATTTCTTCTACT+CGG	+	chr6.1:1299050-1299069	MS.gene82858:CDS	30.0%
	AGTATCTCATTAACAGTAAC+CGG	-	chr6.1:1299120-1299139	None:intergenic	30.0%
	ATTCACTTCCAATTGAATGT+GGG	+	chr6.1:1299348-1299367	MS.gene82858:CDS	30.0%
	CATAGTTCAATCAGTTTAAC+GGG	-	chr6.1:1298886-1298905	None:intergenic	30.0%
	GATGTATTTGTGTTTCCAAT+TGG	+	chr6.1:1299321-1299340	MS.gene82858:CDS	30.0%
	GTTACTGTTAATGAGATACT+AGG	+	chr6.1:1299120-1299139	MS.gene82858:CDS	30.0%
!	TCTTCAAAAACAATTTTGGG+TGG	+	chr6.1:1299518-1299537	MS.gene82858:CDS	30.0%
!	TGTTTTGCAGTTTTTGTGAA+TGG	+	chr6.1:1298997-1299016	MS.gene82858:intron	30.0%
!!	AGAACTTCTGAAGAGATTTT+TGG	-	chr6.1:1299459-1299478	None:intergenic	30.0%
!!	ATCACAAAAGTACTCAACAA+AGG	+	chr6.1:1299297-1299316	MS.gene82858:CDS	30.0%
	AGTATACATACCACCATTCT+TGG	-	chr6.1:1298819-1298838	None:intergenic	35.0%
	ATACTAGGACTAAACACTCT+AGG	+	chr6.1:1299135-1299154	MS.gene82858:CDS	35.0%
	CATTCACTTCCAATTGAATG+TGG	+	chr6.1:1299347-1299366	MS.gene82858:CDS	35.0%
	CTATGATACAAAAGTCTCAC+GGG	+	chr6.1:1298901-1298920	MS.gene82858:intron	35.0%
	GGAAATACATCAAATCCACT+TGG	+	chr6.1:1299081-1299100	MS.gene82858:CDS	35.0%
	TCAAGTGGACAAGAATATAG+TGG	+	chr6.1:1299491-1299510	MS.gene82858:CDS	35.0%
	TTAGCAATAGTGATAACACC+TGG	-	chr6.1:1299417-1299436	None:intergenic	35.0%
	TTCACTTCCAATTGAATGTG+GGG	+	chr6.1:1299349-1299368	MS.gene82858:CDS	35.0%
!	TAGTGATAACACCTGGATTT+TGG	-	chr6.1:1299410-1299429	None:intergenic	35.0%
!	TGAGTACTTTTGTGATGAGA+CGG	-	chr6.1:1299293-1299312	None:intergenic	35.0%
	ACAAGATGCTAAAGCCAAGA+AGG	-	chr6.1:1298741-1298760	None:intergenic	40.0%
	ACTTGGTTCAAAAGTGACAC+CGG	+	chr6.1:1299098-1299117	MS.gene82858:CDS	40.0%
	ATAAGAATCTCAGTGGCTCT+AGG	-	chr6.1:1299219-1299238	None:intergenic	40.0%
	ATCAATGACACCAAGAATGG+TGG	+	chr6.1:1298806-1298825	MS.gene82858:CDS	40.0%
	CAACACGATAAGAATCTCAG+TGG	-	chr6.1:1299226-1299245	None:intergenic	40.0%
	CACTGAGATTCTTATCGTGT+TGG	+	chr6.1:1299224-1299243	MS.gene82858:CDS	40.0%
	CTTGTCCACTTGAAAAGCTT+TGG	-	chr6.1:1299484-1299503	None:intergenic	40.0%
	GAGATTCTTATCGTGTTGGA+GGG	+	chr6.1:1299228-1299247	MS.gene82858:CDS	40.0%
	GCTATCAATGACACCAAGAA+TGG	+	chr6.1:1298803-1298822	MS.gene82858:CDS	40.0%
	TGAGATTCTTATCGTGTTGG+AGG	+	chr6.1:1299227-1299246	MS.gene82858:CDS	40.0%
	TGGAAGTGAATGAGACCAAT+TGG	-	chr6.1:1299339-1299358	None:intergenic	40.0%
	TGTGAATGGCAAATTCTGCA+AGG	+	chr6.1:1299011-1299030	MS.gene82858:CDS	40.0%
	TTCCAATTGAATGTGGGGTA+TGG	+	chr6.1:1299354-1299373	MS.gene82858:CDS	40.0%
	TTCCATACCCCACATTCAAT+TGG	-	chr6.1:1299359-1299378	None:intergenic	40.0%
!	CCACACAAAAGTCTTGTAGA+GGG	-	chr6.1:1298784-1298803	None:intergenic	40.0%
!	TAAGAATCTCAGTGGCTCTA+GGG	-	chr6.1:1299218-1299237	None:intergenic	40.0%
!	TTCTTCTACTCGGTGAAAGA+AGG	+	chr6.1:1299060-1299079	MS.gene82858:CDS	40.0%
!	TTTGTGATGAGACGGTTTTC+TGG	-	chr6.1:1299285-1299304	None:intergenic	40.0%
!!	TCATTTGCTGTAGCAAGCTT+TGG	-	chr6.1:1299039-1299058	None:intergenic	40.0%
!!	TCGTGTTGACTTTGCATCTA+GGG	+	chr6.1:1299170-1299189	MS.gene82858:CDS	40.0%
!!	TCTCACCAAAGCTTTTCAAG+TGG	+	chr6.1:1299476-1299495	MS.gene82858:CDS	40.0%
!!	TTGGAGGGTACTCTATATGT+CGG	+	chr6.1:1299243-1299262	MS.gene82858:CDS	40.0%
	CTTGTTTGTGACAGCCTTCT+TGG	+	chr6.1:1298724-1298743	MS.gene82858:CDS	45.0%
	GAATGTGGGGTATGGAAATG+CGG	+	chr6.1:1299362-1299381	MS.gene82858:CDS	45.0%
	TATCAATGTTGTCGGACGTG+AGG	-	chr6.1:1298932-1298951	None:intergenic	45.0%
!	ACAAAAGTCTTGTAGAGGGC+TGG	-	chr6.1:1298780-1298799	None:intergenic	45.0%
!	CAAAAGTCTTGTAGAGGGCT+GGG	-	chr6.1:1298779-1298798	None:intergenic	45.0%
!	CCCTCTACAAGACTTTTGTG+TGG	+	chr6.1:1298781-1298800	MS.gene82858:CDS	45.0%
!	GCCACACAAAAGTCTTGTAG+AGG	-	chr6.1:1298785-1298804	None:intergenic	45.0%
!!	CGTGTTGACTTTGCATCTAG+GGG	+	chr6.1:1299171-1299190	MS.gene82858:CDS	45.0%
!!	CTCGTGTTGACTTTGCATCT+AGG	+	chr6.1:1299169-1299188	MS.gene82858:CDS	45.0%
!!!	GGTGTCACTTTTGAACCAAG+TGG	-	chr6.1:1299099-1299118	None:intergenic	45.0%
	GGACTTAGCAGCCAAAATCC+AGG	+	chr6.1:1299396-1299415	MS.gene82858:CDS	50.0%
	TTGGCTGCTAAGTCCAGCAA+TGG	-	chr6.1:1299391-1299410	None:intergenic	50.0%
	TTGTAGAGGGCTGGGATCAA+AGG	-	chr6.1:1298771-1298790	None:intergenic	50.0%
	AGGGCTGGGATCAAAGGCAA+AGG	-	chr6.1:1298765-1298784	None:intergenic	55.0%
	GGAAATGCGGTTGCCATTGC+TGG	+	chr6.1:1299375-1299394	MS.gene82858:CDS	55.0%
!!	GCTCTAGGGTGAGTATGTGG+AGG	-	chr6.1:1299204-1299223	None:intergenic	55.0%
!!	GTGGCTCTAGGGTGAGTATG+TGG	-	chr6.1:1299207-1299226	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	1298710	1299551	1298710	ID=MS.gene82858
chr6.1	mRNA	1298710	1299551	1298710	ID=MS.gene82858.t1;Parent=MS.gene82858
chr6.1	exon	1298710	1298827	1298710	ID=MS.gene82858.t1.exon1;Parent=MS.gene82858.t1
chr6.1	CDS	1298710	1298827	1298710	ID=cds.MS.gene82858.t1;Parent=MS.gene82858.t1
chr6.1	exon	1299007	1299551	1299007	ID=MS.gene82858.t1.exon2;Parent=MS.gene82858.t1
chr6.1	CDS	1299007	1299551	1299007	ID=cds.MS.gene82858.t1;Parent=MS.gene82858.t1

Gene Sequence

>MS.gene82858

ATGAAGACTTCAATCTTGTTTGTGACAGCCTTCTTGGCTTTAGCATCTTGTGCCTTTGCCTTTGATCCCAGCCCTCTACAAGACTTTTGTGTGGCTATCAATGACACCAAGAATGGTGGTATGTATACTTTTTCATAATTATTTATTTATTCATCTTTACTCGTTTGGTTCAACCCGTTAAACTGATTGAACTATGATACAAAAGTCTCACGGGTTCAACCTCACGTCCGACAACATTGATATATACATATATAAGTAGAGCATGTTATTTAATTACATGATGAATTTGTTTTGCAGTTTTTGTGAATGGCAAATTCTGCAAGGATCCAAAGCTTGCTACAGCAAATGATTTCTTCTACTCGGTGAAAGAAGGAAATACATCAAATCCACTTGGTTCAAAAGTGACACCGGTTACTGTTAATGAGATACTAGGACTAAACACTCTAGGTATCTCTCTTGCTCGTGTTGACTTTGCATCTAGGGGACTAAATCCTCCACATACTCACCCTAGAGCCACTGAGATTCTTATCGTGTTGGAGGGTACTCTATATGTCGGATTTGTTACTTCTAATCCAGAAAACCGTCTCATCACAAAAGTACTCAACAAAGGAGATGTATTTGTGTTTCCAATTGGTCTCATTCACTTCCAATTGAATGTGGGGTATGGAAATGCGGTTGCCATTGCTGGACTTAGCAGCCAAAATCCAGGTGTTATCACTATTGCTAATGCTGTATTTGGATCTAATCCAAAAATCTCTTCAGAAGTTCTCACCAAAGCTTTTCAAGTGGACAAGAATATAGTGGACAATCTTCAAAAACAATTTTGGGTGGACAACAACTAG

Protein sequence

>MS.gene82858.t1

MKTSILFVTAFLALASCAFAFDPSPLQDFCVAINDTKNGVFVNGKFCKDPKLATANDFFYSVKEGNTSNPLGSKVTPVTVNEILGLNTLGISLARVDFASRGLNPPHTHPRATEILIVLEGTLYVGFVTSNPENRLITKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANAVFGSNPKISSEVLTKAFQVDKNIVDNLQKQFWVDNN