AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82859.t1 MTR_6g005350 92.233 206 16 0 1 206 14 219 5.56e-141 392
MS.gene82859.t1 MTR_6g005340 78.261 207 44 1 1 206 14 220 1.10e-110 316
MS.gene82859.t1 MTR_6g005360 77.778 207 45 1 1 206 14 220 2.45e-110 315
MS.gene82859.t1 MTR_6g005330 77.778 207 45 1 1 206 14 220 4.94e-110 314
MS.gene82859.t1 MTR_6g005310 77.670 206 45 1 1 205 14 219 1.78e-109 313
MS.gene82859.t1 MTR_1g079490 68.750 208 62 3 2 206 15 222 1.07e-92 270
MS.gene82859.t1 MTR_6g005380 70.256 195 56 2 1 193 14 208 6.07e-92 268
MS.gene82859.t1 MTR_5g046410 68.116 207 64 2 2 206 15 221 6.76e-92 268
MS.gene82859.t1 MTR_5g046430 68.116 207 64 2 2 206 15 221 6.76e-92 268
MS.gene82859.t1 MTR_4g017030 68.116 207 64 2 2 206 15 221 6.76e-92 268
MS.gene82859.t1 MTR_4g017050 68.116 207 64 2 2 206 15 221 6.84e-92 268
MS.gene82859.t1 MTR_4g017040 68.116 207 64 2 2 206 15 221 8.51e-92 268
MS.gene82859.t1 MTR_2g019250 68.116 207 64 2 2 206 15 221 1.23e-91 268
MS.gene82859.t1 MTR_1g023770 55.500 200 78 4 5 201 18 209 1.06e-67 206
MS.gene82859.t1 MTR_2g030855 54.500 200 83 5 5 201 18 212 3.89e-63 195
MS.gene82859.t1 MTR_2g031270 55.729 192 77 5 13 201 27 213 3.58e-62 192
MS.gene82859.t1 MTR_2g030895 55.307 179 75 4 27 201 1 178 3.74e-57 179
MS.gene82859.t1 MTR_2g030865 54.190 179 77 4 27 201 1 178 6.20e-55 173
MS.gene82859.t1 MTR_1g085500 45.274 201 103 4 3 200 17 213 6.76e-52 166
MS.gene82859.t1 MTR_7g111240 45.918 196 99 4 9 201 20 211 6.47e-46 151
MS.gene82859.t1 MTR_1g085140 42.211 199 108 4 6 201 21 215 1.31e-44 148
MS.gene82859.t1 MTR_2g031300 50.296 169 60 4 27 192 1 148 1.60e-43 143
MS.gene82859.t1 MTR_7g111240 45.408 196 97 5 9 201 20 208 8.03e-43 143
MS.gene82859.t1 MTR_1g112200 39.706 204 101 6 5 201 19 207 1.58e-36 127
MS.gene82859.t1 MTR_5g036570 41.117 197 108 5 9 201 27 219 1.51e-35 125
MS.gene82859.t1 MTR_1g060120 36.181 199 118 4 9 201 26 221 4.20e-33 119
MS.gene82859.t1 MTR_8g020750 41.765 170 93 3 15 182 25 190 4.32e-33 118
MS.gene82859.t1 MTR_8g020590 36.508 189 113 4 13 199 19 202 1.99e-29 108
MS.gene82859.t1 MTR_2g041550 37.073 205 111 7 3 198 8 203 1.02e-28 107
MS.gene82859.t1 MTR_4g052770 41.606 137 76 3 66 201 72 205 1.29e-28 106
MS.gene82859.t1 MTR_2g041580 36.559 186 111 4 15 198 23 203 1.39e-28 106
MS.gene82859.t1 MTR_2g041570 36.364 187 112 4 15 199 23 204 1.40e-28 106
MS.gene82859.t1 MTR_4g052780 41.606 137 76 3 66 201 72 205 1.85e-28 106
MS.gene82859.t1 MTR_2g036120 36.139 202 122 4 1 201 10 205 3.08e-28 105
MS.gene82859.t1 MTR_8g007300 40.331 181 103 3 9 187 26 203 3.30e-28 105
MS.gene82859.t1 MTR_3g019680 36.098 205 116 6 4 201 8 204 2.73e-27 103
MS.gene82859.t1 MTR_2g086620 41.791 134 74 3 69 201 74 204 8.23e-27 102
MS.gene82859.t1 MTR_2g044040 34.653 202 125 4 1 201 10 205 9.03e-27 102
MS.gene82859.t1 MTR_2g086630 41.791 134 74 3 69 201 74 204 1.12e-26 101
MS.gene82859.t1 MTR_3g019650 35.610 205 117 6 4 201 8 204 1.71e-26 101
MS.gene82859.t1 MTR_2g086640 40.741 135 76 3 68 201 72 203 1.75e-26 101
MS.gene82859.t1 MTR_3g019710 35.149 202 124 4 1 201 10 205 2.07e-26 100
MS.gene82859.t1 MTR_8g020630 34.518 197 122 4 6 201 15 205 2.45e-26 100
MS.gene82859.t1 MTR_8g020650 35.638 188 114 4 15 201 24 205 6.81e-26 99.8
MS.gene82859.t1 MTR_8g020770 37.696 191 110 5 14 201 24 208 7.70e-26 99.8
MS.gene82859.t1 MTR_2g045100 35.821 201 118 5 4 201 13 205 2.41e-25 98.2
MS.gene82859.t1 MTR_8g020670 34.211 190 118 4 13 201 22 205 1.02e-23 94.0
MS.gene82859.t1 MTR_5g031460 31.658 199 129 4 4 201 13 205 2.00e-23 93.2
MS.gene82859.t1 MTR_8g020760 34.737 190 117 4 13 201 24 207 2.37e-23 93.2
MS.gene82859.t1 MTR_8g020763 33.908 174 109 3 30 201 17 186 6.18e-23 91.7
MS.gene82859.t1 MTR_0284s0010 34.171 199 120 5 6 201 15 205 1.26e-22 91.3
MS.gene82859.t1 MTR_7g011340 34.375 192 115 5 13 201 22 205 1.47e-22 90.9
MS.gene82859.t1 MTR_4g010340 34.682 173 107 4 30 201 34 201 1.14e-21 88.6
MS.gene82859.t1 MTR_8g020610 33.333 189 117 6 15 201 22 203 3.52e-21 87.4
MS.gene82859.t1 MTR_3g096330 34.857 175 98 3 2 162 557 729 5.37e-21 90.5
MS.gene82859.t1 MTR_2g019780 31.915 188 119 5 15 201 29 208 2.35e-19 82.8
MS.gene82859.t1 MTR_2g030915 52.000 75 22 1 80 154 1 61 2.11e-18 77.0
MS.gene82859.t1 MTR_3g073170 30.000 190 120 6 15 201 27 206 3.67e-16 73.9
MS.gene82859.t1 MTR_3g019810 30.500 200 124 7 1 198 116 302 9.15e-14 68.9
MS.gene82859.t1 MTR_4g027415 36.719 128 71 4 13 137 22 142 1.89e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82859.t1 AT5G38930 59.694 196 76 2 9 201 26 221 5.90e-71 215
MS.gene82859.t1 AT5G38940 58.163 196 79 2 9 201 26 221 1.56e-70 214
MS.gene82859.t1 AT5G38940 58.163 196 79 2 9 201 50 245 3.44e-70 214
MS.gene82859.t1 AT5G39160 56.650 203 83 4 3 201 18 219 8.46e-70 212
MS.gene82859.t1 AT5G39190 56.650 203 83 4 3 201 18 219 1.10e-69 212
MS.gene82859.t1 AT5G39130 55.665 203 85 4 3 201 18 219 2.72e-68 208
MS.gene82859.t1 AT3G05950 57.353 204 82 4 2 201 19 221 3.43e-68 208
MS.gene82859.t1 AT5G39110 54.680 203 88 3 3 201 18 220 6.71e-68 207
MS.gene82859.t1 AT5G39160 55.172 203 82 4 3 201 18 215 8.84e-67 204
MS.gene82859.t1 AT5G39150 55.276 199 85 4 7 201 21 219 9.31e-67 204
MS.gene82859.t1 AT5G39120 55.276 199 85 4 7 201 21 219 1.93e-66 204
MS.gene82859.t1 AT5G39180 55.276 199 85 4 7 201 21 219 3.02e-66 203
MS.gene82859.t1 AT5G38910 58.673 196 78 3 9 201 24 219 5.21e-66 202
MS.gene82859.t1 AT5G38960 54.455 202 88 2 4 201 21 222 1.09e-65 202
MS.gene82859.t1 AT4G14630 53.465 202 90 3 4 201 21 222 1.65e-64 199
MS.gene82859.t1 AT3G04200 55.392 204 86 4 2 201 20 222 4.98e-62 192
MS.gene82859.t1 AT5G39190 51.741 201 74 3 3 201 18 197 4.77e-60 187
MS.gene82859.t1 AT5G39160 51.244 201 75 3 3 201 18 197 1.19e-59 186
MS.gene82859.t1 AT3G04150 50.980 204 95 4 2 201 19 221 3.98e-58 183
MS.gene82859.t1 AT3G04150 49.074 216 94 4 2 201 19 234 6.35e-58 182
MS.gene82859.t1 AT3G04180 49.268 205 97 5 2 201 19 221 6.02e-55 174
MS.gene82859.t1 AT3G04170 50.000 204 94 5 2 201 19 218 1.13e-54 174
MS.gene82859.t1 AT3G04190 49.020 204 99 4 2 201 19 221 1.17e-54 174
MS.gene82859.t1 AT5G26700 44.059 202 101 5 4 201 16 209 7.83e-49 159
MS.gene82859.t1 AT1G02335 43.137 204 109 4 1 201 16 215 7.85e-49 159
MS.gene82859.t1 AT3G05930 44.670 197 101 4 9 201 23 215 4.70e-48 157
MS.gene82859.t1 AT1G09560 43.284 201 99 5 9 202 24 216 2.75e-47 155
MS.gene82859.t1 AT3G62020 43.284 201 106 5 5 201 19 215 4.73e-46 152
MS.gene82859.t1 AT1G18980 44.388 196 100 5 9 201 29 218 4.14e-43 144
MS.gene82859.t1 AT1G18970 43.367 196 102 5 9 201 29 218 4.56e-43 144
MS.gene82859.t1 AT5G38910 45.918 196 66 4 9 201 24 182 6.27e-41 137
MS.gene82859.t1 AT3G62020 43.575 179 95 4 27 201 10 186 2.42e-40 136
MS.gene82859.t1 AT3G10080 38.614 202 117 4 4 201 21 219 1.15e-36 128
MS.gene82859.t1 AT3G10080 38.614 202 117 4 4 201 92 290 5.62e-36 128
MS.gene82859.t1 AT1G10460 37.186 199 117 5 9 201 19 215 5.17e-34 120
MS.gene82859.t1 AT5G61750 40.884 181 102 3 9 187 19 196 9.93e-33 117
MS.gene82859.t1 AT5G20630 36.364 198 119 4 3 198 12 204 7.76e-29 107
MS.gene82859.t1 AT1G72610 36.170 188 113 4 13 198 19 201 8.67e-28 104
MS.gene82859.t1 AT1G74820 35.052 194 117 5 11 201 38 225 1.13e-27 104
MS.gene82859.t1 AT5G38950 55.738 61 26 1 131 190 2 62 7.48e-13 62.8

Find 46 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATTGTTAAGGAGGTATT+TGG 0.136261 6.1:+1303228 MS.gene82859:CDS
GGAAATGCAGCTGCCATTTC+CGG 0.176460 6.1:+1303177 MS.gene82859:CDS
TCTTCTGGTGTAACAAGTTT+TGG 0.236271 6.1:-1302838 None:intergenic
ACAATCTTCAGAAGCAATTC+TGG 0.265114 6.1:+1303313 MS.gene82859:CDS
TAGTGATAACTCTTGAATTA+TGG 0.314281 6.1:-1303209 None:intergenic
CCCACAGTTAGTGAGATATT+AGG 0.322926 6.1:+1302922 MS.gene82859:CDS
CTTCTGAAGATTGTCAACTA+TGG 0.352917 6.1:-1303304 None:intergenic
AAGAGTTATCACTATTGTTA+AGG 0.372185 6.1:+1303218 MS.gene82859:CDS
GAAATGCAGCTGCCATTTCC+GGG 0.374807 6.1:+1303178 MS.gene82859:CDS
GATGTCTTTGTGTTTCCAAT+TGG 0.380749 6.1:+1303123 MS.gene82859:CDS
CAAAAGTCTTGCAGAGGATT+GGG 0.395175 6.1:-1301869 None:intergenic
CTATTGTTAAGGAGGTATTT+GGG 0.412780 6.1:+1303229 MS.gene82859:CDS
TCGTATTGACTTTGCACCTA+AGG 0.416056 6.1:+1302972 MS.gene82859:CDS
ACAAAAGTCTTGCAGAGGAT+TGG 0.427807 6.1:-1301870 None:intergenic
TGGAAGTGAATAAGACCAAT+TGG 0.438371 6.1:-1303138 None:intergenic
TGAATTATGGCTGTTAAGCC+CGG 0.446254 6.1:-1303196 None:intergenic
ATATTAGGTTTAAATACTCT+TGG 0.452516 6.1:+1302937 MS.gene82859:CDS
ATGGCTGTTAAGCCCGGAAA+TGG 0.455636 6.1:-1303190 None:intergenic
TGTGGAGGATTTAGACCCTT+AGG 0.459506 6.1:-1302988 None:intergenic
TTGGAGGGTACTCTCAATGT+TGG 0.485489 6.1:+1303045 MS.gene82859:CDS
CGTATTGACTTTGCACCTAA+GGG 0.496446 6.1:+1302973 MS.gene82859:CDS
TGAGATACTTATAGTGTTGG+AGG 0.499047 6.1:+1303029 MS.gene82859:CDS
TATTCACTTCCAACATAATG+TGG 0.501651 6.1:+1303149 MS.gene82859:CDS
ATAAGTATCTCAGCGGCTCT+AGG 0.503745 6.1:-1303018 None:intergenic
TATTAACGACATCAAGACTT+GGG 0.520700 6.1:+1301898 MS.gene82859:CDS
CGCTGAGATACTTATAGTGT+TGG 0.521275 6.1:+1303026 MS.gene82859:CDS
ATTCACTTCCAACATAATGT+GGG 0.554540 6.1:+1303150 MS.gene82859:CDS
TCTTCAGAAGCAATTCTGGT+TGG 0.562500 6.1:+1303317 MS.gene82859:CDS
ACCTAATATCTCACTAACTG+TGG 0.565377 6.1:-1302923 None:intergenic
ATTGTCCACTTGAAGAGCCT+TGG 0.568947 6.1:-1303280 None:intergenic
TTCACTTCCAACATAATGTG+GGG 0.575295 6.1:+1303151 MS.gene82859:CDS
TCTCACCAAGGCTCTTCAAG+TGG 0.580497 6.1:+1303275 MS.gene82859:CDS
AGTTATCACTATTGTTAAGG+AGG 0.582820 6.1:+1303221 MS.gene82859:CDS
CCTAATATCTCACTAACTGT+GGG 0.596269 6.1:-1302922 None:intergenic
TTATTAACGACATCAAGACT+TGG 0.601714 6.1:+1301897 MS.gene82859:CDS
ATTACAAAAGTTCTCAACAA+AGG 0.621502 6.1:+1303099 MS.gene82859:CDS
TTCTTCAGAAGTTCTCACCA+AGG 0.626705 6.1:+1303263 MS.gene82859:CDS
ATCACACAAAAGTCTTGCAG+AGG 0.631911 6.1:-1301875 None:intergenic
GAAAACATATCAAGTCCACT+TGG 0.632399 6.1:+1302883 MS.gene82859:CDS
GAGATACTTATAGTGTTGGA+GGG 0.645513 6.1:+1303030 MS.gene82859:CDS
TCACTTCCAACATAATGTGG+GGG 0.647461 6.1:+1303152 MS.gene82859:CDS
ATTAACGACATCAAGACTTG+GGG 0.648171 6.1:+1301899 MS.gene82859:CDS
AGTGTCACTTGCGAACCAAG+TGG 0.679750 6.1:-1302898 None:intergenic
GCGGCTCTAGGATGAGCATG+TGG 0.681614 6.1:-1303006 None:intergenic
GCTCTAGGATGAGCATGTGG+AGG 0.684016 6.1:-1303003 None:intergenic
CAACACTATAAGTATCTCAG+CGG 0.743612 6.1:-1303025 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAATTTATCTAATTTA+AGG - chr6.1:1302527-1302546 None:intergenic 10.0%
!! TTGAAATTTATCTAATTTAA+GGG - chr6.1:1302526-1302545 None:intergenic 10.0%
!! AAATGTATTTGATCTAAATT+TGG - chr6.1:1302459-1302478 None:intergenic 15.0%
!! GTTATAATGTTATAATACAA+TGG - chr6.1:1302763-1302782 None:intergenic 15.0%
!! ATATTAGGTTTAAATACTCT+TGG + chr6.1:1302937-1302956 MS.gene82859:CDS 20.0%
!!! ATCAATTTAATTTTCAGTGT+TGG + chr6.1:1302792-1302811 MS.gene82859:intron 20.0%
! AAGAGTTATCACTATTGTTA+AGG + chr6.1:1303218-1303237 MS.gene82859:CDS 25.0%
! ATTACAAAAGTTCTCAACAA+AGG + chr6.1:1303099-1303118 MS.gene82859:CDS 25.0%
! ATTGACATTCTTTACTTTGA+GGG + chr6.1:1302647-1302666 MS.gene82859:intron 25.0%
! CTATAAAACATCATTTCGTA+AGG + chr6.1:1302141-1302160 MS.gene82859:intron 25.0%
! GATAAAATACATTTGACCTT+TGG - chr6.1:1302437-1302456 None:intergenic 25.0%
! TAGTGATAACTCTTGAATTA+TGG - chr6.1:1303212-1303231 None:intergenic 25.0%
! TGATCTTAGATATAACCAAA+AGG + chr6.1:1302000-1302019 MS.gene82859:intron 25.0%
! TTTAGTGTTTCATATAGTTG+AGG + chr6.1:1302675-1302694 MS.gene82859:intron 25.0%
!! AATTTAAAGAATGAGAGTAC+CGG + chr6.1:1302376-1302395 MS.gene82859:intron 25.0%
ACTGTGAAAAAGAAATCTTC+TGG - chr6.1:1302856-1302875 None:intergenic 30.0%
AGATATAACCAAAAGGTCAA+GGG + chr6.1:1302007-1302026 MS.gene82859:intron 30.0%
AGTTATCACTATTGTTAAGG+AGG + chr6.1:1303221-1303240 MS.gene82859:CDS 30.0%
ATTCACTTCCAACATAATGT+GGG + chr6.1:1303150-1303169 MS.gene82859:CDS 30.0%
GATTGACATTCTTTACTTTG+AGG + chr6.1:1302646-1302665 MS.gene82859:intron 30.0%
TAGATATAACCAAAAGGTCA+AGG + chr6.1:1302006-1302025 MS.gene82859:intron 30.0%
TATTAACGACATCAAGACTT+GGG + chr6.1:1301898-1301917 MS.gene82859:CDS 30.0%
TATTCACTTCCAACATAATG+TGG + chr6.1:1303149-1303168 MS.gene82859:CDS 30.0%
TTATTAACGACATCAAGACT+TGG + chr6.1:1301897-1301916 MS.gene82859:CDS 30.0%
! GGAGTATTTCGCAAATTTAA+AGG - chr6.1:1301977-1301996 None:intergenic 30.0%
! TGAGAACTTTTGTAATGAGA+CGG - chr6.1:1303095-1303114 None:intergenic 30.0%
! TGGTTATATCTAAGATCAGA+TGG - chr6.1:1301998-1302017 None:intergenic 30.0%
!! ACTATTGTTAAGGAGGTATT+TGG + chr6.1:1303228-1303247 MS.gene82859:CDS 30.0%
!! CTATTGTTAAGGAGGTATTT+GGG + chr6.1:1303229-1303248 MS.gene82859:CDS 30.0%
!!! TAATTTTCAGTGTTGGTGAA+CGG + chr6.1:1302799-1302818 MS.gene82859:intron 30.0%
!!! TTGTAATGAGACGGTTTTTT+GGG - chr6.1:1303086-1303105 None:intergenic 30.0%
!!! TTTGTAATGAGACGGTTTTT+TGG - chr6.1:1303087-1303106 None:intergenic 30.0%
ACAATCTTCAGAAGCAATTC+TGG + chr6.1:1303313-1303332 MS.gene82859:CDS 35.0%
ACAATGGATGTGAAGTGTAA+GGG - chr6.1:1302747-1302766 None:intergenic 35.0%
ACCGGTACACTCAAATATAT+TGG + chr6.1:1302394-1302413 MS.gene82859:intron 35.0%
ACCTAATATCTCACTAACTG+TGG - chr6.1:1302926-1302945 None:intergenic 35.0%
ATTAACGACATCAAGACTTG+GGG + chr6.1:1301899-1301918 MS.gene82859:CDS 35.0%
CAACACTATAAGTATCTCAG+CGG - chr6.1:1303028-1303047 None:intergenic 35.0%
CCCAATATATTTGAGTGTAC+CGG - chr6.1:1302398-1302417 None:intergenic 35.0%
CCGGTACACTCAAATATATT+GGG + chr6.1:1302395-1302414 MS.gene82859:intron 35.0%
CCTAATATCTCACTAACTGT+GGG - chr6.1:1302925-1302944 None:intergenic 35.0%
GAAAACATATCAAGTCCACT+TGG + chr6.1:1302883-1302902 MS.gene82859:CDS 35.0%
GATGTCTTTGTGTTTCCAAT+TGG + chr6.1:1303123-1303142 MS.gene82859:CDS 35.0%
GTAAGGGTGGATAATTGTAA+GGG - chr6.1:1302715-1302734 None:intergenic 35.0%
TACAATGGATGTGAAGTGTA+AGG - chr6.1:1302748-1302767 None:intergenic 35.0%
TGGAAGTGAATAAGACCAAT+TGG - chr6.1:1303141-1303160 None:intergenic 35.0%
TGTAAGGGTGGATAATTGTA+AGG - chr6.1:1302716-1302735 None:intergenic 35.0%
TGTACCATAGAATTTGCCAA+AGG + chr6.1:1302418-1302437 MS.gene82859:intron 35.0%
TTCACTTCCAACATAATGTG+GGG + chr6.1:1303151-1303170 MS.gene82859:CDS 35.0%
TTGACCTTTGGCAAATTCTA+TGG - chr6.1:1302425-1302444 None:intergenic 35.0%
! AAATTATGAAGCGAGAGTAC+CGG - chr6.1:1302066-1302085 None:intergenic 35.0%
! CTTCTGAAGATTGTCAACTA+TGG - chr6.1:1303307-1303326 None:intergenic 35.0%
! GAGATACTTATAGTGTTGGA+GGG + chr6.1:1303030-1303049 MS.gene82859:CDS 35.0%
! TCTTCTGGTGTAACAAGTTT+TGG - chr6.1:1302841-1302860 None:intergenic 35.0%
! TGAGATACTTATAGTGTTGG+AGG + chr6.1:1303029-1303048 MS.gene82859:CDS 35.0%
AAAGGTCAAGGGAACTTCTA+CGG + chr6.1:1302018-1302037 MS.gene82859:intron 40.0%
ACAAAAGTCTTGCAGAGGAT+TGG - chr6.1:1301873-1301892 None:intergenic 40.0%
ATCACACAAAAGTCTTGCAG+AGG - chr6.1:1301878-1301897 None:intergenic 40.0%
CAAAAGTCTTGCAGAGGATT+GGG - chr6.1:1301872-1301891 None:intergenic 40.0%
CCCACAGTTAGTGAGATATT+AGG + chr6.1:1302922-1302941 MS.gene82859:CDS 40.0%
CGCTGAGATACTTATAGTGT+TGG + chr6.1:1303026-1303045 MS.gene82859:CDS 40.0%
GTAAGGGTGGATAACTGTAA+GGG - chr6.1:1302731-1302750 None:intergenic 40.0%
TCACTTCCAACATAATGTGG+GGG + chr6.1:1303152-1303171 MS.gene82859:CDS 40.0%
TCTTCAGAAGCAATTCTGGT+TGG + chr6.1:1303317-1303336 MS.gene82859:CDS 40.0%
TGAATTATGGCTGTTAAGCC+CGG - chr6.1:1303199-1303218 None:intergenic 40.0%
TGTAAGGGTGGATAACTGTA+AGG - chr6.1:1302732-1302751 None:intergenic 40.0%
TTCTTCAGAAGTTCTCACCA+AGG + chr6.1:1303263-1303282 MS.gene82859:CDS 40.0%
! TAGAAGTTCCCTTGACCTTT+TGG - chr6.1:1302018-1302037 None:intergenic 40.0%
!! CGTATTGACTTTGCACCTAA+GGG + chr6.1:1302973-1302992 MS.gene82859:CDS 40.0%
!! TCGTATTGACTTTGCACCTA+AGG + chr6.1:1302972-1302991 MS.gene82859:CDS 40.0%
ACTCACAAAGTGAGGTGTAC+CGG + chr6.1:1302044-1302063 MS.gene82859:intron 45.0%
AGGGTGGATAATTGTAAGGG+TGG - chr6.1:1302712-1302731 None:intergenic 45.0%
ATAAGTATCTCAGCGGCTCT+AGG - chr6.1:1303021-1303040 None:intergenic 45.0%
ATGGATGTGAAGTGTAAGGG+TGG - chr6.1:1302744-1302763 None:intergenic 45.0%
ATTGTCCACTTGAAGAGCCT+TGG - chr6.1:1303283-1303302 None:intergenic 45.0%
TACGGTACACTCACAAAGTG+AGG + chr6.1:1302036-1302055 MS.gene82859:intron 45.0%
TGTGGAGGATTTAGACCCTT+AGG - chr6.1:1302991-1303010 None:intergenic 45.0%
TTCCAACATAATGTGGGGGA+TGG + chr6.1:1303156-1303175 MS.gene82859:CDS 45.0%
TTCCATCCCCCACATTATGT+TGG - chr6.1:1303161-1303180 None:intergenic 45.0%
!! TTGGAGGGTACTCTCAATGT+TGG + chr6.1:1303045-1303064 MS.gene82859:CDS 45.0%
AGGGTGGATAACTGTAAGGG+TGG - chr6.1:1302728-1302747 None:intergenic 50.0%
ATGGCTGTTAAGCCCGGAAA+TGG - chr6.1:1303193-1303212 None:intergenic 50.0%
TCTCACCAAGGCTCTTCAAG+TGG + chr6.1:1303275-1303294 MS.gene82859:CDS 50.0%
! GAAATGCAGCTGCCATTTCC+GGG + chr6.1:1303178-1303197 MS.gene82859:CDS 50.0%
! GGAAATGCAGCTGCCATTTC+CGG + chr6.1:1303177-1303196 MS.gene82859:CDS 50.0%
!! AGTGTCACTTGCGAACCAAG+TGG - chr6.1:1302901-1302920 None:intergenic 50.0%
!! GCTCTAGGATGAGCATGTGG+AGG - chr6.1:1303006-1303025 None:intergenic 55.0%
!! GCGGCTCTAGGATGAGCATG+TGG - chr6.1:1303009-1303028 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 1301842 1303350 1301842 ID=MS.gene82859
chr6.1 mRNA 1301842 1303350 1301842 ID=MS.gene82859.t1;Parent=MS.gene82859
chr6.1 exon 1301842 1301920 1301842 ID=MS.gene82859.t1.exon1;Parent=MS.gene82859.t1
chr6.1 CDS 1301842 1301920 1301842 ID=cds.MS.gene82859.t1;Parent=MS.gene82859.t1
chr6.1 exon 1302809 1303350 1302809 ID=MS.gene82859.t1.exon2;Parent=MS.gene82859.t1
chr6.1 CDS 1302809 1303350 1302809 ID=cds.MS.gene82859.t1;Parent=MS.gene82859.t1
Gene Sequence

>MS.gene82859

ATGACATCTTTTGTCTTTACATTTGATCCCAATCCTCTGCAAGACTTTTGTGTGATTATTAACGACATCAAGACTTGGGGTATGTATATATAATAATTTATTTTATTTTTATTAACAGTTGATCGATTTAGTCCTTTAAATTTGCGAAATACTCCATCTGATCTTAGATATAACCAAAAGGTCAAGGGAACTTCTACGGTACACTCACAAAGTGAGGTGTACCGGTACTCTCGCTTCATAATTTTATAAATTAACGATCATTTTTCATGTAAGAAATAGCTATTTTTCAATATTTATATCTATAAAACATCATTTCGTAAGGAAAAAACATCATTTCATTGTTTATAAGAATCATAGTATTTTTTTTTACATATTATCGTAAAAAATAATCAACGTTCTCAATCATGAGTGATAAAAATTCAAAAAAAAAAAATTTTATTTCGTTGACACTTTTGATGAATAAGATTTAGTTATTATAATTATAAGTGATAAAAAAACAATATTTTTCTTCAAAAAACAACTCATTTAACTATTAATTTAAAGAATGAGAGTACCGGTACACTCAAATATATTGGGTGTACCATAGAATTTGCCAAAGGTCAAATGTATTTTATCCAAATTTAGATCAAATACATTTGAATTTTTGCTTATATATAAGACAAAAGCGATTAATTACTTTGTCCCTTAAATTAGATAAATTTCAATCAAATTAGTCAGTTTTTCAAATTTTGATGCAATGAAATATTATTTTATTCGTTTTGATGAATATTTACTACAGAAATATTTTGATGTATGAACTAAATTGATTGACATTCTTTACTTTGAGGGACTAATTTAGTGTTTCATATAGTTGAGGAACTAAATTATCCACCCTTACAATTATCCACCCTTACAGTTATCCACCCTTACACTTCACATCCATTGTATTATAACATTATAACTTTTCTTTGATCAATTTAATTTTCAGTGTTGGTGAACGGAAAATGCTACAAAGATCCAAAACTTGTTACACCAGAAGATTTCTTTTTCACAGTAAAAGAAGAAAACATATCAAGTCCACTTGGTTCGCAAGTGACACTACCCACAGTTAGTGAGATATTAGGTTTAAATACTCTTGGTATCTCTGTTGCTCGTATTGACTTTGCACCTAAGGGTCTAAATCCTCCACATGCTCATCCTAGAGCCGCTGAGATACTTATAGTGTTGGAGGGTACTCTCAATGTTGGTTTTGTTACTTCTAACCCAAAAAACCGTCTCATTACAAAAGTTCTCAACAAAGGAGATGTCTTTGTGTTTCCAATTGGTCTTATTCACTTCCAACATAATGTGGGGGATGGAAATGCAGCTGCCATTTCCGGGCTTAACAGCCATAATTCAAGAGTTATCACTATTGTTAAGGAGGTATTTGGGTCTAATAAAATTTCTTCAGAAGTTCTCACCAAGGCTCTTCAAGTGGACAATACCATAGTTGACAATCTTCAGAAGCAATTCTGGTTGGACAACAATTAG
Protein sequence

>MS.gene82859.t1

MTSFVFTFDPNPLQDFCVIINDIKTWVLVNGKCYKDPKLVTPEDFFFTVKEENISSPLGSQVTLPTVSEILGLNTLGISVARIDFAPKGLNPPHAHPRAAEILIVLEGTLNVGFVTSNPKNRLITKVLNKGDVFVFPIGLIHFQHNVGDGNAAAISGLNSHNSRVITIVKEVFGSNKISSEVLTKALQVDNTIVDNLQKQFWLDNN